KR20160007954A - composition for preventing and alleviating laser-induced skin damage and cosmetic comprising the same - Google Patents

Abstract

The present invention relates to a composition for preventing and alleviating laser-induced skin hypersensitive reaction operated for skin care or treatment of skin lesion. Provided is a composition for preventing and alleviating laser-induced skin hypersensitive reaction operated for skin care or treatment of skin lesion, for inhibiting HSP 70 expression in skin cells, inhibiting proliferation of microvascular cells, inhibiting movement of microvascular cells, and inhibiting vasodilation and/or vascular formation, so as to prevent and/or alleviate skin hypersensitive reaction and/or skin damage such as facial erythema, skin pigmentation, skin pain, pruritus, hypertrophic cicatrix, etc. which can be generated by the laser used for skin care and/or treatment of skin lesion.

Description

[0001] The present invention relates to a composition for preventing or ameliorating a skin irritation caused by a laser, which is used for the treatment of skin lesions or for cosmetic purposes, and a cosmetic composition using the composition for preventing and alleviating laser-

The present invention relates to a composition for preventing or ameliorating a skin hypersensitivity reaction by a laser, which is used for the treatment of skin lesions or for cosmetic purposes. The present invention relates to a composition for preventing skin irritation Prevention of skin irritation and / or skin damage, such as skin irritation and / or skin damage, such as skin irritation and / or skin irritation And compositions for improvement.

The rapid development of the IT industry and sports culture, the development of new forms of collective housing lifestyle and lifestyle tools, and the quality of life associated with health and beauty are prioritizing the social atmosphere, which greatly increases skin burns in a variety of causes and forms. For example, there are skin irritations caused by burns such as disasters, fires, chemical contact, etc., as well as skin lesions caused by laser and high frequency medical devices such as light therapy for skin lesions and cosmetic purposes .

As soon as the skin is exposed to heat, the protein is denatured, coagulated, damaged, and tissue necrosis is induced. Even at a low temperature of 44 ° C or lower, long-term contact destroys the tissue. At 44 ° C to 55 ° C, the cell's destruction rate doubles with every 1 ° C rise.

When skin burns, inflammation is naturally induced in the body. The clinical symptoms of inflammation itself disappear within 24 to 48 hours, but metabolism and immune response persist until skin regeneration, and various The continuous immune response by the mediator occurs, which causes various side effects for a long time. In other words, even if the site of burning is complete, the scars that remain after the treatment will have a prolonged inflammation, over proliferation of fibroblasts and over production of collagen fibers ). For example, hypertrophic scars (scars), skin deformations due to overgrowth of fibroblasts and collagen fibers, skin irritation, skin pruritus (pruritus), skin rash and pain resulting from neovascularization and overgrowth of inflammatory cells, And construction.

Korean Patent Laid-Open No. 10-2009-0030995 discloses a composition for preventing or treating hypertrophic scarring and keloid, which contains sialyl oligosaccharide as an active ingredient.

Korean Patent No. 10-1099550 of the prior art discloses a composition for burn treatment characterized by containing bee venom as an active ingredient.

In the medical field, the laser was first used for the purpose of treatment for ophthalmology, hemostasis of the retinal vascularization due to diabetes and the like. Since then, development and development of lasers has been repeatedly used in almost all medical fields such as dermatology, surgery, and otolaryngology.

Of these, laser treatment for skin lesion treatment and cosmetic purpose has been rapidly expanding the scope of treatment, in line with basic desire of human beings to live healthy and beautifully according to the recent appearance and aging.

Furthermore, recently, at-home skin care devices that manage skin directly at home using a medical aesthetic device have been greatly expanded in the United States and Europe, and skin management using laser has been continuously growing .

According to a 2011 report released by Klein & Company, an international market research organization, the home skin care market has grown to a market volume close to $ 1 billion in 2011, followed by a remarkable growth over the next five years And it is predicted that equipment using laser light used to remove or reduce skin spots and wrinkles among the skin care management apparatuses will be able to grow by 50% per year.

The laser differs in the nature (wavelength) of the light absorbed by the color. Using this principle, diseases with different color from normal skin can selectively destroy the desired tissue by using a laser light that is well absorbed in the color of these lesions.

Specifically, by destroying vascular lesions using laser light of a specific wavelength, it is possible to treat flaky nevi, capillary vasculature, hemangioma, facial flushing, and red acne scarring, and by destroying skin pigment lesions such as hemoglobin and melanocytes Freckles, dullness, black spots, and tattoos.

In addition, different laser mediums and wavelengths can be used to treat various skin lesions such as hair follicles, hair follicles, scarring, scleroderma, osteoporosis through skin destruction, skin wrinkles, Treatment and skin care purposes can be achieved.

Since the laser shows a good therapeutic effect, it is utilized in various skin lesion treatment and skin care fields.

But not just good effects, there are various skin side effects that accompany the skin laser irradiation.

Basically, when light is absorbed into the skin, heat is generated. The cells or tissues constituting living organisms are damaged by heat and damaged when the temperature rises. That is, various side effects are caused while affecting the tissue to be treated and the surrounding normal skin tissue.

In addition, after laser treatment, the treated tissue should be supplied with nutrients necessary for regeneration and recovery. In this process, a lot of new blood vessels are formed, resulting in various skin irritation reactions accompanied with erythema do. In some cases, scabs may develop and if proper management is not performed, pigmentation, itching, skin pain, hypertrophic scarring may occur with prolonged periods, or worse with keloid skin.

Therefore, there is a need for a product and / or method that can reduce the recovery period after laser surgery or prevent and improve various skin irritation reactions that may occur after the procedure.

DISCLOSURE OF THE INVENTION The present invention has been made in order to solve the above problems, and the first problem to be solved by the present invention is to provide a method for treating skin lesions and / Inhibition of HSP70 expression, inhibition of microvascular cell proliferation, inhibition of microvascular cell migration, vasodilatation and / or proliferation of skin cells in order to prevent and / or improve skin hypersensitivity reaction and / or skin damage, Or a composition for preventing or ameliorating a skin hypersensitivity reaction by a laser which is performed for the treatment or cosmetic purposes of a skin lesion inhibiting angiogenesis.

The second problem to be solved by the present invention is to provide a cosmetic preparation for preventing or ameliorating a skin hypersensitivity reaction, which is performed for treatment of a skin lesion having excellent efficacy or safety by using an extract extracted from a natural product or for cosmetic purposes.

The present invention for solving the above-mentioned first problem relates to a composition for preventing or ameliorating a skin hypersensitive reaction by a laser, which is performed for the treatment of skin lesions or for cosmetic purposes, and is characterized by including an onion fermented extract.

According to a preferred embodiment of the present invention, a composition for preventing or ameliorating a skin hypersensitivity reaction by a laser, which is performed for the treatment of skin lesions or for cosmetic purposes, is a composition for preventing redness, skin pigmentation, , Pruritus, and hypertrophic scars can be prevented and improved.

According to another preferred embodiment of the present invention, the laser for the prevention and improvement of skin sensitization by the laser treatment as a therapeutic or cosmetic purpose of skin lesions, the composition is used as a skin disease therapeutic or cosmetic purposes, CO ₂ laser , Nd-YAG laser, Er-YAG laser, Alexandrite laser, He-Ne laser, Intense Pulsed Light (IPL), non-invasive laser or high frequency laser.

According to another preferred embodiment of the present invention, the composition for preventing or ameliorating the skin hypersensitivity reaction by the laser, which is performed for treatment of skin lesions or for cosmetic purposes, may be used for cosmetics, medicines and quasi-drugs.

According to another preferred embodiment of the present invention, the concentration of the onion fermentation extract of the composition for preventing or ameliorating the skin irritation caused by laser treatment for treatment of skin lesions or for cosmetic purposes may be 50 to 10,000 ppm.

According to another preferred embodiment of the present invention, a composition for preventing or ameliorating the skin hypersensitivity reaction by a laser, which is performed for the treatment of skin lesions or for cosmetic purposes, comprises the expression of Heat Shock Protein (HSP) Can be suppressed.

According to another preferred embodiment of the present invention, a composition for preventing or ameliorating a skin hypersensitivity reaction by a laser, which is performed for treatment of skin lesions or for cosmetic purposes, can inhibit proliferation or migration of skin microvascular cells.

According to another preferred embodiment of the present invention, a composition for preventing or ameliorating a skin hypersensitivity reaction performed by a laser for treatment of skin lesions or for cosmetic purposes can inhibit vasodilation and angiogenesis of skin cells.

According to another preferred embodiment of the present invention, the onion fermentation extract of a composition for preventing or ameliorating a skin hypersensitivity reaction by a laser, which is performed for the treatment of skin lesions or for cosmetic purposes, comprises (1) (2) fermenting the onion broth by inoculating the medium with lactic acid bacteria, (3) culturing the medium containing the fermented onion broth with a C ₁ -C ₄ alcohol solvent to obtain an alcohol solvent extract, And (4) filtering the resulting alcohol solvent extract, followed by drying to obtain an onion fermentation extract.

According to another preferred embodiment of the present invention, when preparing the onion fermented extract contained in the composition for preventing or ameliorating the skin irritation caused by laser treatment, The medium of step may contain from 0.1 to 10% by weight of onion.

According to another preferred embodiment of the present invention, when preparing the onion fermented extract contained in the composition for preventing or ameliorating the skin irritation caused by the laser, which is performed for the treatment of skin lesions or for cosmetic purposes, The medium of the present invention includes at least one selected from a carbon source, a nitrogen source and trace elements, and the carbon source includes glucose, sucrose, maltose, fructose, Wherein the nitrogen source comprises at least one selected from the group consisting of yeast extract, lactose, xylose, galactose and arabinose, yeast extract, Peptone, beef extract, soybean protein degradation product, and the like.

The trace element may be selected from the group consisting of sodium acetate, potassium phosphate, diammonium citrate, magnesium sulfate (MgSO ₄ ), manganese sulfate (MnSO ₄ ), zinc sulfate , ZnSO ₄ ) and copper sulfate (CuSO ₄ ).

According to another preferred embodiment of the present invention, when preparing the onion fermented extract contained in the composition for preventing or ameliorating the skin irritation caused by the laser, which is performed for the treatment of skin lesions or for cosmetic purposes, The medium of onion is 0.01-10% by weight, glucose is 0.1-5% by weight, yeast extract is 0.1-2% by weight, potassium phosphate is 0.01-0.2% by weight, sodium acetate 0.01 to 0.5 wt% of diammonium citrate, 0.001 to 0.01 wt% of magnesium sulfate (MgSO ₄ ), 0.001 to 0.05 wt% of manganese sulfate (MnSO ₄ ) 0.0001 to 0.01% by weight of zinc sulfate (ZnSO ₄ ), 0.0001 to 0.01% by weight of copper sulfate (CuSO ₄ ), and the balance of purified water.

According to another preferred embodiment of the present invention, when preparing the onion fermented extract contained in the composition for preventing or ameliorating the skin irritation caused by laser treatment, Step may further include sterilizing the medium at 90 to 121 DEG C for 15 to 30 minutes.

According to another preferred embodiment of the present invention, when preparing an onion fermented extract contained in a composition for preventing or ameliorating a skin hypersensitivity reaction by a laser, which is performed for the treatment of skin lesions or for cosmetic purposes, It may be a strain of at least one of lactobacillus rhamnosus, lactobacillus casei, lactobacillus plantarum, lactobacillus brevis and lactobacillus acidophilus. have.

According to another preferred embodiment of the present invention, when preparing the onion fermented extract contained in the composition for preventing or ameliorating the skin irritation caused by the laser, The inoculation amount of the lactic acid bacteria may be 10 ⁵ to 10 ⁶ cfu / ml at the initial stage of inoculation.

According to another preferred embodiment of the present invention, when preparing the onion fermented extract contained in the composition for preventing or ameliorating the skin irritation caused by the laser, Is an aqueous solution of ethanol in an amount of 20 to 90% by weight, and the extraction in the step (3) can be performed for 12 to 72 hours using the aqueous ethanol solution.

According to another preferred embodiment of the present invention, when preparing the onion fermented extract contained in the composition for preventing or ameliorating the skin irritation caused by laser treatment, , The alcohol solvent extract which has been filtered may be concentrated in vacuo at a temperature of 40 to 80 ° C and then dried by vacuum drying, spray drying or freeze drying.

Next, the present invention for solving the above-mentioned second problem relates to cosmetic preparation for prevention and improvement of skin hypersensitivity reaction performed by laser for treatment of skin lesions or for cosmetic purposes, and includes onion fermented extract.

According to a preferred embodiment of the present invention, cosmetic formulations for preventing or ameliorating the skin irritation caused by laser treatment for the treatment of skin lesions or for cosmetic purposes are formulated into emulsions, lotions, creams, lotions, essences, packs, .

According to another preferred embodiment of the present invention, the cosmetic preparation for prevention and improvement of the skin irritation caused by laser treatment for the treatment of skin lesions or cosmetic purposes is glycerin, butylene glycol, But are not limited to, dipropylene glycol, carboxyvinylpolymer, polysorbate 80, hydrogenated caster oil, glyceryl stearate, cetearyl alcohol, And may further include at least one selected from cetyl ethylhexanoate, glycyrrhiza inflate extract and lecithin.

According to another preferred embodiment of the present invention, a cosmetic preparation for prevention and improvement of skin irritation caused by laser treatment for skin lesion treatment or cosmetic purposes comprises glycerin 500 , 700 to 1100 parts by weight of butylene glycol, 1100 to 1300 parts by weight of dipropylene glycol, 50 to 70 parts by weight of carboxyvinylpolymer, ), 90 to 110 parts by weight of cetyl alcohol, 700 to 900 parts by weight of hydrogenated caster oil, 90 to 110 parts by weight of glyceryl stearate, 180 to 220 parts by weight of cetearyl alcohol, 500 to 700 parts by weight of cetyl ethylhexanoate, 10 to 30 parts by weight of glycyrrhiza inflate extract and 10 to 30 parts by weight of lecithin.

The compositions and cosmetic formulations for preventing or ameliorating skin irritation caused by laser treatment for the treatment or cosmetic use of the skin lesions of the present invention can be used for treatment of skin lesions and / Inhibition of HSP70 expression, inhibition of microvascular cell proliferation, inhibition of microvascular cell migration, vasodilatation and / or proliferation of skin cells in order to prevent and / or improve skin hypersensitivity reaction and / or skin damage such as skin pain, pruritus and hypertrophic scarring, Or inhibit angiogenesis.

Figure 1 is a graph showing the quercetin content of a typical onion.
FIG. 2 is a result of measuring cytotoxicity on a medium containing onion fermented extract according to a preferred embodiment of the present invention.
FIG. 3 shows the results of measuring the inhibitory activity of HSP70 on a medium containing onion fermented extract according to a preferred embodiment of the present invention.
FIG. 4 is a graph showing the cell proliferation inhibitory activity of a medium containing onion fermented extract according to a preferred embodiment of the present invention.
FIG. 5 is a microscopic observation of cell migration inhibitory activity on a medium containing an onion fermentation extract according to a preferred embodiment of the present invention.
FIG. 6 shows microscopic observation of vasodilation and angiogenesis inhibitory activity on a medium containing onion fermented extract according to a preferred embodiment of the present invention.
FIGS. 7 to 10 are cross-sectional views illustrating a method of treating a skin lesion according to a preferred embodiment of the present invention or a cosmetic preparation for improving and preventing skin irritation caused by a laser, This is the result of clinical experiment.

Hereinafter, the present invention will be described in more detail.

As described above, the treatment and cosmetic treatment of skin lesions using a laser is primarily intended to destroy skin tissue or skin pigment other than normal skin color. In this process, skin tissue is destroyed and destroyed due to heat energy applied to the skin tissue Cells cause nonbacterial inflammatory response, which can cause side effects such as flushing, swelling, pain, bleeding, and the like. In addition, side effects from the laser cause mild to moderate skin damage such as redness of the skin, skin pigmentation, skin pain, pruritus (itching) and hypertrophic scars (scarring). Accordingly, there is a need for a product and / or method that can reduce the recovery period after laser surgery, and products and / or methods that minimize such side effects are needed.

Accordingly, the present invention has been made to solve the above-mentioned problems through a composition for preventing or ameliorating the skin hypersensitivity reaction by the laser, which is performed for the treatment of skin lesions or for cosmetic purposes. As a result, it is possible to provide a skin sensitizing agent for treating skin lesions having excellent effect of inhibiting HSP 70 expression, inhibiting microvascular cell proliferation, inhibiting microvascular cell migration, vasodilation and / or angiogenesis in skin cells, A composition for prevention and improvement of the reaction and a formulation thereof.

In addition, the composition for prevention and improvement of skin irritation caused by laser treatment for cosmetic purposes or treatment of skin lesions of the present invention and its cosmetic formulation can be used as an extract derived from a natural product, that is, an extract obtained by fermenting onion blood, Is excellent.

The composition for preventing or ameliorating the skin hypersensitivity reaction by the laser, which is performed for the treatment or cosmetic purposes of the skin lesions of the present invention, includes an onion fermented extract. Accordingly, the composition for preventing or ameliorating the skin hypersensitivity reaction by the laser, which is performed for the treatment or cosmetic purpose of the skin lesion of the present invention, can be used for skin sensitization and / or skin damage , Redness, skin pigmentation, skin pain, pruritus (itching), and hypertrophic scarring (scarring). Such skin hypersensitivity reaction and / or skin damage may be remnants or damage that may occur after treatment, even if the site of burning of the skin has been completed. For example, hypertrophic scars (scarring) can be caused by prolonged inflammation, over proliferation of fibroblasts, and over production of collagen fibers , Redness, skin pigmentation, pruritus (pruritus), skin rash, and pain can be caused by neovascularization and overexpression of inflammatory cells.

The laser is used for skin lesion treatment or cosmetic purposes and can be used for CO ₂ laser, Nd-YAG laser, Er-YAG laser, Alexandrite laser, He-Ne laser, Intense Pulsed Light (IPL), non- have. CO ₂ lasers belong to far infrared rays with a wavelength of 10,600 nm and are mainly absorbed by tissue moisture. The Er-YAG laser has a wavelength of 2,940 nm and is absorbed about 10 times as much moisture as the CO ₂ laser, which is less heat-transferring to the surrounding tissues and is not well-tolerated. Noninvasive lasers are lasers that exhibit skin peeling effects by transferring heat energy to only the collagenous fibers in the dermis, without damaging the epidermis. A high frequency laser is a laser that can transmit heat to the inside of the skin, that is, the skin supporting ligaments, fatty tissue and deep skin layers. However, the laser is not limited thereto, and may include all the lasers irradiated in a medical device used for skin lesion treatment and / or cosmetic purposes in a dermatological hospital or home.

The composition for preventing or ameliorating the skin hypersensitivity reaction by the laser, which is used for the treatment of skin lesions or for cosmetic purposes of the present invention, may be any one of cosmetics, medicines and quasi-drugs. In other words, the composition of the present invention can be used for cosmetic purposes, pharmaceutical use, or quasi-drug use.

As mentioned above, the composition for preventing or improving the skin hypersensitivity reaction by the laser, which is performed for the treatment or cosmetic purpose of the skin lesion of the present invention, includes the onion fermentation extract, and the expression of HSP (Heat Shock Protein) Inhibition of microvascular cell proliferation and / or migration of microvascular cells, as well as an effect of inhibiting vasodilatation and / or angiogenesis of skin microvascular cells.

FIG. 3 is a graph showing the results of experiments in which the concentration of onion fermented extract was diluted 10 times as much as the concentration of onion fermented extract, which can be contained in cosmetics, medicines or quasi-drugs. 3, it can be seen that the onion fermentation extract of the present invention has an effect of inhibiting HSP70 expression in skin cells.

HSP is a protein synthesized when cells, tissues or individuals are exposed to temperatures higher than the physiological temperature. TSP-b1 is activated by overexpression of HSP, inducing vasodilation and neovascularization, inducing inflammatory reaction, promoting fibroblast synthesis, Stimulation of collagen fiber synthesis and the like are continuously activated. In other words, the heat shock response increases the activity of TGF-b1 in hypertrophic scars (scars) accompanied by inflammatory reactions, resulting in a variety of side effects. 3, it can be seen that the onion fermentation extract of the present invention has an effect of inhibiting HSP70 expression in skin cells.

4 to 6 show results obtained by diluting the onion fermentation extract at a concentration 10 times higher than that of the onion fermentation extract, which may be contained in cosmetics, medicines or quasi-drugs. The inhibition of cell proliferation FIG. 4 shows that the onion fermentation extract of the present invention shows cell proliferation inhibitory effect, and the inhibitory activity of the skin cell migration on the medium containing the onion fermented extract of the present invention is confirmed by a microscope 5, it can be seen that the onion fermentation extract of the present invention has an effect of inhibiting cell migration. 6, which shows microscopic observation of vasodilation and angiogenesis inhibitory activity of skin cells in a medium containing the onion fermentation extract of the present invention, the onion fermentation extract of the present invention inhibits vasodilation and angiogenesis inhibition The effect can be confirmed.

In particular, when the concentration of the onion fermentation extract of the present invention is in the range of 50 to 10,000 ppm, preferably 300 to 5,000 ppm, excellent suppression of HSP 70 expression, inhibition of microvascular cell proliferation, inhibition of microvascular cell migration, Respectively.

Accordingly, when the onion fermented extract of the present invention is present at a concentration of 50 to 10,000 ppm, it may be very advantageous to maximize the inhibition of HSP70 expression, inhibition of microvascular cell proliferation, inhibition of microvascular cell migration, vasodilation and angiogenesis inhibition.

 If the concentration of the onion fermentation extract of the present invention is less than 50 ppm, the effect of suppressing HSP 70 expression, inhibition of microvascular cell proliferation, inhibition of microvascular cell migration, vasodilatation and angiogenesis is insufficient, There is a possibility of toxicity.

The onion fermentation extract contained in the composition for preventing or ameliorating the skin irritation caused by the laser treatment of the skin lesion of the present invention or the cosmetic purpose of the present invention is prepared by the following method.

The step of producing the onion fermented extract comprises the steps of (1) preparing a medium containing dry pulverized onion broth, (2) fermenting the onion broth by inoculating the medium with the lactic acid bacteria, (3) filtering the step of extracting the culture medium containing a C ₁ ~ C ₄ alcohol solvent, to give the alcohol solvent extract, and (4) the resulting alcohol solvent extracts were then dried and a step of obtaining a blood fermentation onion extract. Thus, the produced onion fermented extract has excellent effect in suppressing the expression of HSP (Heat Shock Protein) 70, inhibiting microvascular cell proliferation, inhibiting microvascular cell migration, and proliferation and / or angiogenesis in skin cells see.

First, as step (1), a medium containing onion blood is prepared.

The onion contains quercetin 4-glucoside, quercetin 4,7-diglycoside, quercetin 3,7-diglycoside, quercetin agglomerate, Flavonoids such as quercetin aglycone, isorhamnetin monoglycoside and kaempferol monoglycoside are contained. Of these, 80% are quercetin aglycoside, quercetin aglycoside, Monoglycoside, and quercetin glycone have been reported.

Quercetin is a type of flavonoid, and it has been reported that these flavonoids inhibit neovascularization of skin as well as protect blood vessels and capillaries, inhibit lipid peroxidation, and inhibit allergies and skin diseases. Furthermore, there is a report that the expression of HSP is inhibited at the time when epithelization is completed.

The onion used in step (1) of the present invention uses the onion blood among the onions. The term onion, which is the term used in the present invention, refers to the red crust of the onion. In other words, when manufacturing the medium containing the onion poultice ground in step (1), the onion poultice contained in the medium can be used only on the outermost skin of the onion.

As mentioned above, the onion contains a substance called quercetin, and the quercetin content of the onion is as shown in Fig. 1 is a graph showing the quercetin content of onion. Referring to FIG. 1, the outer skin of the onion, rather than the inner skin, contains an increasing amount of quercetin. Specifically, the quercetin content of the onion crust was 322.0 mg / 100 g, the inside of the crust was 27.2 mg / 100 g in the first ply, 14.0 mg / 100 g in the second ply, 11.5 mg / 100 g in the third ply, It contains 100g. As a result, the onion fermentation extract of the present invention using the onion blood, which is the outer shell of the onion without using the inner skin of the onion, contains a high concentration of quercetin. In other words, the use of the onion blood of the present invention is excellent in inhibiting the expression of HSP (Heat Shock Protein) 70, inhibiting microvascular cell proliferation, inhibiting microvascular cell migration, vasodilation and / or angiogenesis in skin cells Inhibition of HSP (Heat Shock Protein) 70 expression inhibition, inhibition of microvascular cell proliferation, inhibition of microvascular cell migration, vasodilatation and / or angiogenesis in the skin cells for the purpose of the present invention if the inner skin of the onion is used instead of onion It may be difficult to achieve the excellence of the effect.

The onion used in the present invention may be used after being washed 8 to 12 times in a washing machine, followed by drying and roasting at 60 to 100 ° C for 8 to 12 hours, followed by pulverization.

The medium of step (1) may contain 0.1-10% by weight, preferably 2-8% by weight of onion broth.

The culture medium may include at least one selected from a carbon source, a nitrogen source, and trace elements.

The carbon source may be selected from the group consisting of glucose, sucrose, maltose, fructose, lactose, xylose, galactose and arabinose. Or more, and may preferably include glucose.

The nitrogen source may comprise at least one selected from yeast extract, peptone, beef extract, and soybean protein degradation product, and may preferably comprise a yeast extract.

Trace elements are sodium acetate, potassium phosphate, diammonium citrate, magnesium sulfate (MgSO ₄ ), manganese sulfate (MnSO ₄ ), zinc sulfate (zinc sulfate) ZnSO ₄ , and copper sulfate (CuSO ₄ ).

The medium of step (1) may be selected from the group consisting of onion extract, yeast extract, sodium acetate, potassium phosphate, diammonium citrate, magnesium sulfate (MgSO ₄ ) Manganese sulfate (MnSO ₄ ), zinc sulfate (ZnSO ₄ ), copper sulfate (CuSO ₄ ), and balance purified water.

As mentioned above, the medium of step (1) may contain 0.1-10% by weight, preferably 2-8% by weight of onion.

Next, the medium of step (1) may contain 0.1 to 5% by weight, preferably 0.5 to 2% by weight of glucose, and 0.1 to 2% by weight, preferably 0.3 to 2% by weight, % Of sodium acetate, 0.01 to 0.5% by weight, preferably 0.03 to 0.1% by weight of sodium acetate, and 0.01 to 0.2% by weight, preferably 0.05 to 0.2% by weight, of potassium phosphate And may contain 0.01 to 0.5% by weight, preferably 0.03 to 0.1% by weight, of ammonium citrate (diammonium citrate).

The medium of step (1) may contain 0.001 to 0.1% by weight, preferably 0.003 to 0.01% by weight of magnesium sulfate and 0.001 to 0.05% by weight, preferably 0.003 to 0.01% by weight, of manganese sulfate. And may contain 0.001 to 0.01% by weight, preferably 0.002 to 0.005% by weight of zinc sulfate, and 0.001 to 0.01% by weight, preferably 0.002 to 0.005% by weight, of copper sulfate.

Finally, the medium of step (1) may contain purified water. The purified water is added to the medium in an amount of 0.1-10 wt% onion, 0.1-5 wt% glucose, 0.1-2 wt% yeast extract, potassium phosphate 0.01 to 0.2% by weight of sodium acetate, 0.01 to 0.5% by weight of sodium acetate, 0.01 to 0.5% by weight of diammonium citrate, 0.001 to 0.01% by weight of magnesium sulfate (MgSO ₄ ) _{sulfate, MnSO 4) 0.001 ~ 0.05} % by weight zinc sulfate (zinc sulfate, ZnSO ₄₎ containing the exception of 0.0001 ~ 0.01%, copper sulfate _{(copper sulfate, CuSO 4) 0.0001} ~ 0.01 weight%, that comprise a residual amount .

Preparing a medium containing the pulverized onion broth in the step (1), and sterilizing the medium. At this time, the sterilization treatment may be carried out at a temperature of 90 to 121 ° C for 15 to 30 minutes, preferably at a temperature of 100 to 110 ° C for 20 to 25 minutes.

Next, in step (2), lactic acid bacteria are inoculated on the medium prepared in step (1) to ferment onion pods. The lactic acid bacteria inoculated in step (2) are cultured at 25 to 45 ° C. for 12 to 72 hours, preferably 30 to 40 ° C. for 36 to 60 hours to ferment a culture medium containing onion blood, If the temperature is lower than 25 ° C, fermentation will not occur easily. If the temperature is higher than 45 ° C, lactic acid bacteria that are weak against heat may die.

The lactic acid bacteria may be selected from the group consisting of Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus brevis (Lactobacillus sp. lactobacillus brevis, and lactobacillus acidophilus.

In step (2), the inoculation amount of the lactic acid bacteria may be 10 ⁵ to 10 ⁶ cfu / ml at the initial stage of inoculation. If the initial number of lactic acid bacteria inoculated in step (2) is less than 10 ⁵ cfu / ml, the culture time of the lactic acid bacteria becomes longer, and 10 ⁶ cfu / ml If it is exceeded, it may be an economical burden to the production of seed bacterium.

Next, as the step (3), the medium containing the onion blood fermented in the step (2) is extracted with a C ₁ -C ₄ alcohol solvent to obtain an alcohol solvent extract. The alcohol solvent used as the extraction solvent of the medium containing onion blood is C ₁ -C ₄ And lower alcohols may be used, but ethanol may be preferably used. The alcohol solvent of the present invention means alcohol, and may be 100% alcohol or mixed with other solvent. In this case, water, methyl alcohol, ethyl acetate, chloroform, hexane and the like can be preferably used as other solvents. When water is used as a solvent for the alcohol solvent, 20 to 90% by weight, preferably 40 to 80% It is very advantageous to maximize the extraction efficiency. In other words, less than 20% by weight of C ₁ -C ₄ If an aqueous alcohol solution is used, onion components contained in the alcohol can be reduced, and if it exceeds 90 wt%, the onion components contained in the alcohol can be economically burdened without increasing the amount of onion components.

In extracting the culture medium containing the onion broth fermented in step (2) of the present invention with a C ₁ -C ₄ alcohol solvent, it is possible to extract the medium at room temperature, and those having ordinary skill in the art Various conventional extraction methods that are self-explanatory may be used. In addition, in the extraction of the medium containing the onion blood fermented in step (2) with a C ₁ -C ₄ alcohol solvent, the extraction temperature is 10 to 40 ° C, preferably 20 to 30 ° C, At 12 to 72 hours, preferably 24 to 48 hours, and the number of times of extraction may be 1 to 5 times, preferably 2 to 3 times.

Next, as the step (4), the alcohol solvent extract obtained in the step (3) is filtered and dried to obtain an onion fermented extract.

The filtration in step (4) is performed to remove the cell component or the insoluble component contained in the alcohol solvent extract obtained in the step (3). The filtration is carried out using a filter press or a membrane filter, the filtration method using a centrifuge can be carried out and various conventional filtration methods obvious to those skilled in the art can be carried out.

The alcohol solvent extract filtered in step (4) may be concentrated in vacuo at a temperature of 40 to 80 ° C, preferably 50 to 70 ° C.

The vacuum concentrated alcohol solvent extract is subjected to a drying step and may be dried by various conventional drying methods apparent to those skilled in the art and preferably by vacuum drying, They may be dried by spray drying or freeze drying.

The onion fermented extract according to the present invention produced by the above-described method can inhibit the expression of HSP (Heat Shock Protein) 70, inhibit microvascular cell proliferation, inhibit microvascular cell migration, vasodilatation and / or angiogenesis in skin cells The effect of suppressing the effect is excellent, as described above.

Furthermore, the present invention provides a cosmetic formulation for preventing or ameliorating a skin hypersensitivity reaction by a laser, which is performed for the treatment of skin lesions or for cosmetic purposes.

The cosmetic preparation for preventing or ameliorating the skin irritation caused by the laser, which is used for the treatment of skin lesions or for cosmetic purposes of the present invention, is a cosmetic preparation for skin adhesion type such as lotion, lotion, cream, lotion, essence, pack, gel or mist It can have a formulation.

In addition to the onion fermented extract, other ingredients may be selected and mixed according to the purpose of use. For example, it is possible to use, as a blending component, a preservative component, a moisturizer, an emollient agent, a surfactant, an organic and inorganic pigment, an organic powder, an antiseptic, a bactericide, an antioxidant, a plant extract, a pH adjuster, . However, the blending components are not limited thereto, and other blending components can be blended to the extent that the objects and effects of the present invention are not impaired.

The cosmetic preparation for prevention and improvement of skin hypersensitivity reaction by the laser, which is performed for the treatment of skin lesion of the present invention or for cosmetic purpose, is glycerin, butylene glycol, dipropylene glycol, But are not limited to, polymers such as carboxyvinylpolymer, polysorbate 80, hydrogenated caster oil, glyceryl stearate, cetearyl alcohol, cetyl ethylhexanoate, , Glycyrrhiza inflate extract, and lecithin. By including such components, cosmetic formulations for preventing or ameliorating the skin irritation caused by the laser, which is performed for the treatment or cosmetic purposes of the skin lesions of the present invention, can be used for skin sensitization and / Can be used as a cream product for prevention and improvement of damage. In other words, the treatment of skin lesions of the present invention, which can be used as a cream product for preventing or improving skin irritation caused by a laser, which is performed for the treatment of skin lesions or for cosmetic purposes, Can prevent and / or improve skin irritation and / or skin damage such as redness, skin pigmentation, skin pain, pruritus (pruritus) and hypertrophic scars (scarring), moisturizing and / Whitening and other ingredients can be added to the composition to provide a skin cosmetic benefit.

The glycerin which moisturizes the skin may contain 500 to 700 parts by weight, preferably 550 to 650 parts by weight, based on 100 parts by weight of the onion fermented extract, and may be used as a solvent, a viscosity reducing agent, a flavoring agent and a skin conditioner The butylene glycol may contain 900 to 1100 parts by weight, preferably 950 to 1050 parts by weight, based on 100 parts by weight of the onion fermented extract.

The dipropylene glycol used as a humectant, antimicrobial agent and fermentation inhibitor may contain 1,100 to 1,300 parts by weight, preferably 1,150 to 1,250 parts by weight, based on 100 parts by weight of the onion fermented extract, and the carboxyvinyl The polymer may include 50 to 70 parts by weight, preferably 55 to 65 parts by weight, based on 100 parts by weight of the onion fermented extract.

The polysorbate 80 used as an emulsifier may contain 90 to 110 parts by weight, preferably 95 to 105 parts by weight, based on 100 parts by weight of the onion fermented extract, and the hardened castor oil used as a softener may be onion fermented And 700 to 900 parts by weight, preferably 750 to 850 parts by weight, based on 100 parts by weight of the extract.

The glyceryl stearate used as a detergent or a solubilizing aid may contain 90 to 110 parts by weight, preferably 95 to 105 parts by weight, based on 100 parts by weight of the onion fermented extract, and may contain emulsifying stabilizers, opacifiers, The amount of cetearyl alcohol used as a thickening agent may be in the range of 180 to 220 parts by weight, preferably 190 to 210 parts by weight, based on 100 parts by weight of the onion fermented extract.

The cetyl ethylhexanoate used as a skin softening agent may contain 500 to 700 parts by weight, preferably 550 to 650 parts by weight, based on 100 parts by weight of the onion fermented extract, and may be used for whitening, The licorice extract having excellent detoxifying action, antibacterial action and anti-inflammatory action may contain 10 to 30 parts by weight, preferably 15 to 25 parts by weight, based on 100 parts by weight of the onion fermented extract, and lecithin, which is used as an emulsifier, 10 to 30 parts by weight, preferably 15 to 25 parts by weight, based on 100 parts by weight of the onion fermented extract.

In addition to the above-mentioned materials, cosmetic preparations for preventing or improving skin irritation caused by laser treatment for skin lesions or cosmetic purposes of the present invention may further include purified water, preservatives, and / or flavoring agents.

As mentioned above, the cosmetic preparation for prevention and improvement of the skin irritation caused by laser treatment for the treatment of skin lesions or for cosmetic purposes of the present invention inhibits the expression of HSP (Heat Shock Protein) 70 in skin cells, inhibits microvascular cell proliferation , Skin irritation such as redness of skin, skin pigmentation, skin pain, pruritus (itching) and hypertrophic scars (scar), and / or skin damage Is effective in preventing and / or ameliorating the following diseases.

Referring to FIG. 7 to FIG. 10, which is a clinical test of skin redness after applying a cosmetic preparation for preventing or improving skin irritation caused by a laser to treat skin lesions of the present invention or for cosmetic purposes, When a cosmetic preparation for preventing or ameliorating the skin irritation caused by laser treatment is applied to skin for treatment of skin lesion or cosmetic purpose after laser treatment for lesion treatment and / or cosmetic purpose, It is possible to prevent and / or ameliorate skin redness, which is one of reaction and / or skin damage. In other words, when the cosmetic preparation for preventing or ameliorating the skin irritation caused by the laser is applied to the treated skin, the skin lesion of the present invention can be used for the treatment or cosmetic purposes of the present invention The prevention of skin irritation caused by the laser to be performed, and the prevention of skin irritation are prevented when the cosmetic formulation for improvement is not applied to the skin and / or when a general cream product not containing the onion fermented extract of the present invention is applied to the skin And / or can be improved.

Hereinafter, the present invention will be described in detail with reference to examples. However, these examples are intended to further illustrate the present invention, and the scope of the present invention is not limited to these examples.

[ Example ]

Example 1. Preparation of onion fermented extract

(1) Preparation of a medium containing onion blood

Remove the surface of the onion purchased from the corporation of the Samnet Food and Agriculture Company, Inc. and obtain only the onion's surface (hereinafter referred to as "onion pie") and wash it in the washing machine about 10 times. Then, it is dried and roasted at 80 DEG C for 10 hours and then pulverized.

The medium containing the pulverized onion broth was prepared as shown in Table 1 below.

Ingredients Weight% (w / w) Ingredients Weight% (w / w) Crushed onion p 8 Magnesium sulfate 0.012 Glucose 0.5 Manganese sulfate 0.003 Yeast extract 0.5 Zinc sulfate 0.001 Potassium phosphate 0.25 Copper sulfate 0.001 Sodium acetate 0.12 Purified water 90.493 Ammonium citrate 0.12

The medium prepared as shown in Table 1 is subjected to sterilization treatment at a temperature of 121 캜 for 20 minutes.

(2) Onion pine  Fermentation

Lactobacillus rhamnosus strain, which is a lactic acid bacterium, was inoculated into the medium prepared in Example 1-1, and the lactic acid bacteria were cultured at a temperature of 38 캜 for 48 hours to ferment onion pods. At this time, the inoculation amount of lactic acid bacteria is 10 ⁶ cfu / ml at the initial stage of inoculation.

(3) Extraction with an alcohol solvent to obtain an alcohol solvent extract

5.34 L of 95% by weight ethanol was added to 1 L of the medium containing the onion broth fermented in Example 1-2, and the final alcohol concentration was adjusted to 80% by weight. The resulting mixture was extracted at room temperature for 48 hours to obtain 6.34 L of an alcohol solvent extract ≪ / RTI >

(4) The alcohol solvent extract was filtered and dried to obtain Onion p  Obtain fermented extract

6.34 L of the alcohol solvent extract obtained in Example 1-3 was subjected to filter press to remove the cell component and the insoluble component. Thereafter, the mixture was concentrated in vacuo at a temperature of 60 DEG C and then dried by freeze drying to obtain about 25 g of onion fermentation extract.

Example  2. Manufacture of cream products for the prevention and improvement of skin irritation caused by lasers treated for skin lesions or cosmetic purposes

(1) Add 0.3 g of carboxyvinylpolymer to 75 g of purified water and mix. 3 g of glycerin, 5 g of butylene glycol, 6 g of dipropylene glycol and 0.1 g of lecithin were added and the mixture was heated to 80 ° C to prepare an aqueous phase.

(2) 0.5 g of polysorbate 80, 4 g of hydrogenated caster oil, 0.5 g of glyceryl stearate, 1 g of cetearyl alcohol, 3 g of cetyl ethylhexanoate was added, and the mixture was heated to 80 ° C to prepare an oil phase.

(3) To the oil phase prepared in (2), the water phase prepared in (1) is slowly added slowly to the vessel while stirring and mixing.

(4) After the temperature is lowered to 40 캜, 0.5 g of preservative, 0.5 g of flavor, 0.5 g of onion fermented extract and 0.1 g of licorice extract are added to the container and stirred to prepare a cream product for prevention and improvement of skin hypersensitivity reaction.

Comparative Example 1

Was prepared in the same manner as in Example 1 except for step (2) of Example 1 (fermentation of onion pod).

Comparative Example  2

In the same manner as in Example 1 except that the onion inner skin stripped of onion was used in step (1) of Example 1, and the step (2) of Example 1 (fermentation of onion sauce) was used.

Comparative Example 3

Was prepared in the same manner as in Example 1, except that 0% by weight ethanol, that is, 100% by weight of purified water was used as an extraction solvent instead of the 80% by weight ethanol aqueous solution.

Comparative Example 4

The same ingredients as in Example 2 except for the onion fermented extract included in Example 2 were mixed to prepare a cream product for preventing or ameliorating the skin irritation caused by laser treatment for skin lesions or cosmetic purposes.

Preparation Example 1. Cell culture

For cell culture, human dermal microvascular endothelial cells (HDMECs) derived from human dermal tissue were purchased from Innoprot, Spain.

Microvascular cells were plated in 75T flasks and cultured at 37 ° C under 5% CO ₂ . The culture medium used was 5% FBS (fetal bovine serum), 1 volume% antibiotic, 1% bFGF (basic fibroblast grouth factor) and ECM medium (endothelial cell medium-innoprot) containing EGF , And the culture medium was changed every 3 days. When 85% by volume of the cells were cultured in the flask, the cells were incubated. After one or more passages, microvessel cells in a stable state were used for the experiment.

Experimental Example  1: Cytotoxicity confirmation test

96-well cell culture in the microvessels prepared in Example 1 to a culture vessel under 1 × 10 ⁴ cell / ㎖ each well (well) 37 ℃ dispensing, 5 vol% CO ₂ condition for stabilization and to ensure a 24-by 200㎕ Time. The onion blood fermentation extracts prepared after 24 hours in Example 1 to each well (well) of the culture vessel 50ppm, 100ppm, 200ppm, 300ppm, was added by 500ppm and 800ppm concentration following 37 ℃, under a 5 vol.% CO ₂ conditions, 24 20 μl of an MTS sample (CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay kit, Cat no. G5421, Promega) was added thereto, and the mixture was incubated for 2 hours at 37 ° C. in a 5 volume% CO ₂ incubator After the reaction, absorbance was measured at 492 nm. The viability ratio of microvascular cells was calculated based on the control group to which the onion fermentation extract prepared in Example 1 was not added in the culture container, and the results are shown in FIG.

As can be seen in FIG. 2, the onion fermented extract added at a concentration of 800 ppm showed slight toxicity to microvascular cells, but it was not at a significant level. The onion paprika extract added at concentrations of 50 ppm, 100 ppm, 200 ppm, 300 ppm and 500 ppm It was confirmed that the fermented extract did not show toxicity to microvascular cells.

FIG. 2 is a cytotoxicity experiment conducted at a concentration 10 times thinner than the concentration of the onion fermentation extract which can be contained in cosmetics, medicines or quasi-drugs. When preparing cosmetics, medicines or quasi-drugs including onion fermented extract, Considering toxicity, it can be deduced that it is advantageous to use less than 5000ppm.

Experimental Example  2 : HSP ( Heat Shock Protein ) Expression Inhibitory Effect Identification Test

Fibroblast and HaCat cells were cultured in the same manner as Preparation Example 1, respectively. Subsequently, the cultured Fibroblast and HaCat cells were seeded in 200-μl aliquots into each well of a 96-well culture container to have a concentration of 5 × 10 ⁴ cells / ml, and then each well Was added. Thereafter, the cells were cultured for 24 hours at 37 ° C under 5% CO _2, and the effect of inhibiting the expression of HSP 70 was confirmed by RNA extraction.

Each well was incubated with a well in which the fermented extract of Onion fermentation prepared in Example 1 of the present invention was added as a sample at concentrations of 100 ppm and 500 ppm, respectively, and the onion prepared in Comparative Example 1 The extracts were wells added at concentrations of 100 ppm and 500 ppm, respectively.

The results of inhibition of HSP70 expression in Fibroblast and HaCat cells in each well are shown in FIG.

As shown in FIG. 3, in the well-treated Fibroblast and HaCat cells treated with the concentrations of 100 ppm and 500 ppm, respectively, of the fermented onion extract prepared in Comparative Example 1, HSP 70 was expressed in almost the same manner as the control group, It was confirmed that the expression of HSP 70 was significantly inhibited in the wells of Fibroblast and HaCat cells treated with the fermented extract of onion of Example 1 at concentrations of 100 ppm and 500 ppm.

Based on this, it can be seen that the onion fermented extract of the present invention can remarkably suppress the expression of HSP70 in skin cells.

FIG. 3 shows HSP expression inhibition experiments at a concentration 10 times diluted with the concentration of onion fermented extract, which can be contained in cosmetics, medicines or quasi-drugs. In the case of producing cosmetic products containing onion fermented extract, It can be deduced that it is advantageous to use it at 5000 ppm or less in consideration of the expression inhibition surface and the like.

Experimental Example  3: Microvascular cell  Proliferation inhibition confirmation test

96-well under each well (well) 37 ℃, 5 vol% CO ₂ condition in order to stabilize the frequency division by 200㎕ in the microvascular cell culture prepared in Example 1 in the culture vessel so that the 1 × 10 ⁴ cell / ㎖ 24 Time. After culturing for 24 hours, discard the medium from the upper layer and wash the lower layer medium containing microvascular cells with 1 × PBS (Phosphate buffer saline). After washing, 180 .mu.l of a medium containing 5% FBS (fetal bovine serum) was added to the well of the negative control, and 5% FBS and 2 ng / ml bFGF (basic fibroblast grouth factor) , And 2 ng / ml of EGF (epidermal growth factor).

Add 20 μl of sample to each well except the negative control wells of the 96-well culture vessel. After the under, a 96-well culture plate 37 ℃, 5% CO ₂ condition were cultured for 48 hours. After 48 hours, the upper layer of the medium was discarded, the lower layer containing microvessel cells was washed with 5% by volume of PBS, and the medium was replaced with DMEM (Dulbecco's modified eagle's medium) containing 5% FBS. 20 μl of a sample (CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay kit, Cat no. G5421, Promega) was added to each well and reacted for 2 hours at 37 ° C in a 5 volume% CO ₂ incubator. Respectively. The survival ratio of cells in each well was then calculated based on the negative control wells.

Each well was a well in which the fermented extract of onion fermentation prepared in Example 1 of the present invention was added as a sample at concentrations of 50 ppm, 100 ppm, 200 ppm, 300 ppm, 400 ppm, and 500 ppm, respectively, as a positive control well A well in which the fermented onion fermentation extract prepared in Example 1 was not added as a sample, a well in which the extract of the inner shell of the onion prepared in Comparative Example 2 was added as a sample at concentrations of 100 ppm and 200 ppm, 3 was added to the wells at concentrations of 100 ppm and 200 ppm, respectively.

The results of measuring cell proliferation inhibitory activity of microvascular cells in each well are shown in FIG.

As can be seen from FIG. 4, when the fermented extract of fermented onion of Example 1 was added at a concentration of 50 to 500 ppm, the wells significantly inhibited the excessive proliferation of microvascular cells when compared with the positive control group And showed a cell proliferation inhibitory effect higher than that of the well to which Comparative Example 2 or Comparative Example 3 was added.

On the basis of this, it can be seen that the onion fermentation extract of the present invention can inhibit cell proliferation of skin microvascular cells. In particular, when the onion fermentation extract of the present invention is added at a concentration of 100 ppm to 500 ppm, Can be greatly suppressed.

By inhibiting the proliferation of microvascular cells overexpressed by stimulation, it can reduce the redness of skin caused by this.

4 is a graph showing cell proliferation inhibition experiments at a concentration 10-fold diluted from the concentration of the onion fermentation extract which can be contained in cosmetics, medicines or quasi-drugs. In the production of cosmetics, medicines or quasi-drugs containing onion fermented extract, Considering the cell activation surface and the like, it can be deduced that it is advantageous to use it at 5000 ppm or less.

Experimental Example  4 : Microvascular cell  Cell migration inhibition confirmation test

96-well cell culture in the microvessels prepared in Example 1 in the culture vessel under 1 × 10 ⁵ cell / ㎖ the each well (well) 37 ℃, 5 vol% CO ₂ condition for stabilization divides by 15 so that the 200㎕ Time. After incubation for 15 hours, the cells were scratched with 200 μl tip and washed with PBS. Negative control 180 μl of a medium containing 5% FBS (fetal bovine serum) was added to the wells, and 5% FBS, bFGF (basic fibroblast grouth factor) 2 ng / ml and EGF epidermal growth factor) 2 ng / ml.

Add 20 μl of sample to each well except the negative control wells of the 96-well culture vessel. Thereafter, the 96-well culture vessel was incubated at 37 DEG C under 5 vol% CO ₂ for 9 hours. Then, the degree of cell migration was observed through a microscope (Olympus IX53-100 magnification).

In each well, the onion fermentation extract prepared in Example 1 of the present invention was added as a sample at concentrations of 0, 100 ppm, 200 ppm, 300 ppm, and 500 ppm.

The results of measuring the cell migration inhibitory activity of microvascular cells in each well are shown in FIG.

As can be seen from FIG. 5, when the fermented extract of Onion Fermentation Extract of Example 1 was added at a concentration of 100 to 500 ppm, the movement of microvascular cells was significantly inhibited when compared with the positive control, there was.

Based on this, it can be seen that the onion fermentation extract of the present invention can inhibit the cell migration of the microvascular cells of the skin, and if the movement of the blood vessel cells which are pushed around the damaged skin is suppressed, the skin redness around the damaged skin can be reduced have.

5 is a graph showing cell migration inhibition experiments at a concentration 10 times lower than that of onion fermented extract, which may be contained in cosmetics, medicines or quasi-drugs. When preparing cosmetics, medicines or quasi-drugs containing onion fermented extract, Considering the cell activation surface and the like, it can be deduced that it is advantageous to use it at 5000 ppm or less.

Experimental Example  5: Verification test for vasodilation and angiogenesis inhibition

100 쨉 l of a cell adhesion protein (Growth factor reduced matrigel) was dispensed into a 96-well culture container and coated at 37 째 C for 30 minutes.

96-well negative control wells (well) of the culturing vessel 450㎕ to each well (well) so that 3.6 × 10 ⁴ cell / ㎖ diluted microvascular cells in a medium containing 5 vol% FBS (fetal bovine serum) by frequency division, and a positive control with 5 vol% FBS and bFGF (basic fibroblast grouth factor) 2 ng / ㎖, EGF (epidermal growth factor) diluted microvascular cells with containing the 2 ng / ㎖ medium 3.6 × 10 ⁴ cell / Ml in each well. Then add 50 μl of each sample. Thereafter, the 96-well culture vessel was incubated at 37 DEG C under 5 volume% CO ₂ for 24 hours, and the degree of vasodilation and angiogenesis was observed through a microscope (Olympus IX53-100 magnification).

 The wells are the wells in which the fermented extract onion fermentation prepared in Example 1 of the present invention was added as a sample at concentrations of 0, 100 ppm, 200 ppm, 300 ppm, and 500 ppm.

The results of measuring vascular dilation and angiogenesis inhibitory activity of microvascular cells in each well are shown in FIG.

As shown in FIG. 6, microscopic observation was made on inhibition of blood vessel dilation and angiogenesis of microvascular cells in wells added with concentration of 100 ppm to 500 ppm of the fermented extract of onion fermentation of Example 1.

Based on this, it can be seen that the onion fermented extract of the present invention can inhibit vascular expansion and angiogenesis of skin cells.

FIG. 6 is a graph showing blood vessel dilation and blood vessel formation inhibition at a concentration 10 times more diluted than the concentration of onion fermented extract, which can be contained in cosmetics, medicines or quasi-drugs, and is a cosmetic composition containing a fermented onion extract, It can be deduced that it is advantageous to use it at 5000 ppm or less in consideration of the cell activity and the like at the time of production.

As described above, the onion fermentation extract of the present invention inhibits the expression of HSP (Heat Shock Protein) 70, inhibits microvascular cell proliferation, inhibits microvascular cell migration and / or angiogenesis in skin cells, It is obvious that it is a material highly likely to be used as a composite material capable of preventing and / or improving skin irritation and / or skin damage such as hypertrophic scars, pruritus, redness, skin pigmentation,

Experimental Example  6: Laser-induced skin Glowing  Occurrence inhibition confirmation clinical trial

(1) Subjects were selected for healthy subjects who did not have severe or chronic physical diseases including skin diseases.

(2) The entire face of the subject was irradiated with a laser (Platon factional CO ₂ laser, Dr. Med) for 8 minutes with a minimum dose (10.6 μm wavelength, 2.5 J energy) capable of causing skin fever and redness .

(3) Immediately after the laser irradiation on the entire face of the subject, half of the entire face of the subject was treated with the skin lesion prepared in Example 2 or the prevention of skin irritation caused by the laser And half of all the faces of the other subjects were coated with a skin irritant caused by laser treatment for the treatment of skin lesions not containing the onion fermented extract prepared in Comparative Example 4 or for cosmetic purposes A cream product for preventing and improving the reaction was applied.

(4) After 60 minutes, the degree of redness and fever of the subject's skin was measured using an infrared camera (ULIR vision TI 120, Sine Calibration).

(5) The skin temperature of the face of the subject confirmed by the measurement and the visual acuity were examined for improvement of redness.

The results of measurement of the facial skin condition of the subject as described above are shown in FIGS. 7 to 10. FIG.

As shown in Figs. 7 to 10, Experiments 1 to 4 show that the cream product for prevention and improvement of the skin hypersensitivity reaction performed by the laser for the treatment or the cosmetic purpose of the skin lesion of Example 2 is applied to the laser irradiated skin When applied, the skin is treated with a creamy product for prevention or improvement of skin irritation caused by laser treatment, which is performed for the treatment of skin lesions not containing the onion fermentation extract of Comparative Example 4 or for cosmetic purpose, Respectively. In other words, after all the subjects (subjects 1 to 4) have increased the facial skin temperature by the laser treatment, they can prevent the skin irritation caused by the laser treatment for the treatment of the skin lesion of Example 2 or for the cosmetic purpose, The area to which the improvement cream product is applied is the skin skin lesion which does not include the onion fermentation extract of Comparative Example 4 among the face skin, or the cosmetic product is applied for the cosmetic purpose. The skin temperature was measured lower than the area.

As described above, the onion fermentation extract of the present invention inhibits the expression of HSP70 in skin cells, inhibits microvascular cell proliferation, microvascular cell migration, vasodilation and / or angiogenesis in skin cells. Therefore, A formulation capable of preventing and / or improving skin hypersensitivity and / or skin damage, typically redness of skin, by applying a cosmetic formulation for prevention and improvement of skin hypersensitivity reaction by a laser to be performed for cosmetic purpose It is obvious that it is a material which is likely to be utilized.

[ Manufacturing example ]

Preparation Example 1: Preparation for the prevention and improvement of skin irritation caused by laser treatment for treatment of skin lesions or for cosmetic purposes Manufacturing example

The present invention provides a method of preventing or ameliorating a skin irritation caused by a laser, which is performed for the treatment of skin lesions including the onion fermentation extract or for cosmetic purposes, but the present invention is not limited thereto It is.

Manufacturing example  1-1. cream

Onion Fermented Extract 0.5 g

Glycerin 3 g

5 g of butylene glycol

Dipropylene glycol 6 g < RTI ID = 0.0 >

0.3 g of carboxyvinylpolymer

Polysorbate 80 (0.5 g)

Hydrogenated caster oil 4 g

0.5 g of glyceryl stearate

1 g of cetearyl alcohol

3 g of cetyl ethylhexanoate < RTI ID = 0.0 >

0.1 g of glycyrrhiza inflate extract

0.1 g of lecithin

0.5 g of preservative

Spices 0.5 g

Purified water 75 g

After mixing the ingredients, the ingredients were mixed according to a conventional cream-form product manufacturing process. In addition, the mixing ratio of the purified water and the preservative and / or fragrance may be arbitrarily changed.

Manufacturing example  1-2. ample

Onion Fermented Extract 0.5 g

Dipropylene glycol (propylene glycol) 7 g

5 g of 1,3-butylene glycol (1,3-butylene glycol)

Polysorbate 80 (3 g)

Dipotassium glycyrrhizate 0.5 g < RTI ID = 0.0 >

Allantoin 0.5 g

0.5 g of panthenol

Glycerin 3 g

0.5 g of preservative

Spices 0.5 g

Purified water 79 g

After mixing the ingredients, the ingredients were mixed according to conventional ampoule product manufacturing methods. Furthermore, the mixing ratio of the purified water and the preservative and / or fragrance may be arbitrarily changed.

Manufacturing example  1-3. skin

Onion Fermented Extract 0.5 g

5 g of 1,3-butylene glycol (1,3-butylene glycol)

Glycerin 4 g

Polysorbate 80 (2 g)

0.05 g of tocopherol acetate acetate

0.5 g of nicotinic acid amide

Dipotassium glycyrrhizate 0.5 g < RTI ID = 0.0 >

Allantoin 0.5 g

0.5 g of pyridoxine hydrochloride hydrochloride

0.5 g of preservative

Spices 0.5 g

Purified water 85.45 g

After mixing the ingredients, the ingredients were mixed according to the usual skin-type product manufacturing method. Furthermore, the mixing ratio of the purified water and the preservative and / or fragrance may be arbitrarily changed.

Manufacturing example  1-4. pack

Onion Fermented Extract 0.5 g

5 g of glycerin

2 g of butylene glycol

Cellulose gum 1 g

0.2 g of carboxyvinylpolymer

1 g of polysorbate 80 (polysorbate 80)

Allantoin 0.3 g

0.5 g of panthenol

Arginine 0.3 g

40 g hydrogenated caster oil (PEG-40 hydrogenated caster oil)

Licorice extract 0.1 g

0.1 g of lecithin

0.5 g of preservative

Spices 0.5 g

Purified water 87.7 g

After mixing the components, the components were mixed according to a conventional pack-type product manufacturing method. Furthermore, the mixing ratio of the purified water and the preservative and / or fragrance may be arbitrarily changed.

Manufacturing example  1-5. Lotion

Onion Fermented Extract 0.5 g

Glycerin 3 g

5 g of butylene glycol

Erythritol 1 g

0.3 g of carboxyvinylpolymer

0.3 g of xanthan gum

0.5 g of hydrogenated caster oil

0.5 g of glyceryl stearate

1 g of cetearyl alcohol

3 g of cetyl ethylhexanoate < RTI ID = 0.0 >

0.8 g of PEG-100 stearate (PEG-100 stearate)

0.2 g of arginine

Licorice extract 0.1 g

0.1 g of lecithin

0.5 g of preservative

Spices 0.5 g

Purified water 82.7 g

After mixing the ingredients, the ingredients were mixed according to conventional lotion-type product manufacturing methods. Furthermore, the mixing ratio of the purified water and the preservative and / or fragrance may be arbitrarily changed.

Manufacturing example  1-6. essence

Onion Fermented Extract 0.5 g

Glycerin 3 g

5 g of butylene glycol

Erythritol 1 g

0.15 g of carboxyvinylpolymer

0.2 g of xanthan gum

0.5 g of glyceryl stearate

1 g of cetearyl alcohol

0.15 g of arginine

Licorice extract 0.1 g

0.1 g of lecithin

0.5 g of preservative

Spices 0.5 g

Purified water 87.3 g

After mixing the ingredients, the ingredients were mixed according to a conventional method of producing an essence-type product. Furthermore, the mixing ratio of the purified water and the preservative and / or fragrance may be arbitrarily changed.

Claims (21)

A composition for preventing or ameliorating a skin hypersensitivity reaction by a laser, which is carried out for the treatment or cosmetic purposes of a skin lesion, which comprises an onion fermented extract. The method according to claim 1,
Characterized in that at least one selected from the group consisting of redness, skin pigmentation, skin pain, pruritus, and hypertrophic scars occurring in the skin by the laser treatment is prevented and ameliorated. A composition for preventing and improving hypersensitivity reactions. The method according to claim 1,
The laser is used for treating skin lesions or for cosmetic purposes and is a CO ₂ laser, an Nd-YAG laser, an Er-YAG laser, an Alexandrite laser, a He-Ne laser, an IPL (Intense Pulsed Light) Characterized by the treatment of skin lesions or prevention of skin irritation caused by laser treatment for cosmetic purposes, and / RTI > The method according to claim 1,
A composition for preventing or ameliorating a skin hypersensitivity reaction by a laser, which is used for the treatment of skin lesions or for cosmetic purposes, characterized in that the composition is used for any one of a cosmetic, a medicine and a quasi-drug. The method according to claim 1,
Wherein the concentration of the onion fermentation extract is 50 to 10,000 ppm. The composition for preventing or ameliorating a skin hypersensitivity reaction by laser treatment for the treatment of skin lesions or for cosmetic purposes. 6. The method of claim 5,
A composition for preventing or ameliorating a skin hypersensitivity reaction by a laser for the treatment of skin lesions or for cosmetic purposes, characterized by suppressing the expression of Heat Shock Protein (HSP) 70 in skin cells. 6. The method of claim 5,
The prevention of skin irritation caused by the laser treatment for the treatment of skin lesions or for cosmetic purposes, characterized by inhibiting proliferation or migration of skin cells, / RTI > 6. The method of claim 5,
A composition for preventing or ameliorating a skin hypersensitivity reaction by laser treatment for the treatment of skin lesions or for cosmetic purposes, characterized by inhibiting vascular expansion and angiogenesis of skin cells. The method according to claim 1,
The onion fermentation extract
(1) preparing a medium containing dry pulverized onion pod;
(2) inoculating the medium with lactic acid bacteria to ferment the onions;
(3) extracting a culture medium containing fermented onion blood with a C ₁ -C ₄ alcohol solvent to obtain an alcohol solvent extract; And
(4) filtering the obtained alcohol solvent extract and drying to obtain an onion fermented extract;
The composition for preventing or ameliorating a skin hypersensitivity reaction by a laser for treatment of skin lesions or for cosmetic purposes. 10. The method of claim 9,
Wherein the medium of step (1) comprises 0.1 to 10% by weight of onion blood. The composition for preventing or ameliorating a skin hypersensitivity reaction by laser treatment for cosmetic purposes. 10. The method of claim 9,
The medium of step (1) comprises at least one selected from a carbon source, a nitrogen source, and trace elements,
The carbon source may be selected from the group consisting of glucose, sucrose, maltose, fructose, lactose, xylose, galactose and arabinose. Comprising at least one species,
Wherein the nitrogen source comprises at least one selected from the group consisting of yeast extract, peptone, beef extract,
The trace element may be selected from the group consisting of sodium acetate, potassium phosphate, diammonium citrate, magnesium sulfate (MgSO ₄ ), manganese sulfate (MnSO ₄ ), zinc sulfate , ZnSO ₄ ), and copper sulfate (CuSO ₄ ). The composition for preventing or ameliorating a skin hypersensitivity reaction by a laser for treatment of skin lesions or for cosmetic purposes. 12. The method of claim 11,
The medium of step (1) comprises 0.01 to 10% by weight of onion, 0.1 to 5% by weight of glucose, 0.1 to 2% by weight of yeast extract, 0.01 to 0.2% by weight of potassium phosphate, 0.01 to 0.5% by weight of sodium acetate, 0.01 to 0.5% by weight of diammonium citrate, 0.001 to 0.01% by weight of magnesium sulfate (MgSO ₄ ), 0.001 to ₅ % by weight of manganese sulfate (MnSO ₄ ) 0.05% by weight of zinc sulfate, 0.0001 to 0.01% by weight of zinc sulfate (ZnSO ₄ ), 0.0001 to 0.01% by weight of copper sulfate (CuSO ₄ ) and the balance of purified water. For preventing or ameliorating a skin hypersensitivity reaction. 10. The method of claim 9,
Wherein the step (1) further comprises a step of sterilizing the medium at 90 to 121 ° C for 15 to 30 minutes to treat skin lesions or prevent skin irritation caused by laser irradiation / RTI > 10. The method of claim 9,
The lactic acid bacteria of step (2) may be selected from the group consisting of Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus brevis and Lactobacillus acidophilus and lactobacillus acidophilus. The composition for preventing or ameliorating a skin hypersensitivity reaction by a laser is used for the treatment or cosmetic purposes of a skin lesion. 14. The method of claim 13,
Wherein the inoculation amount of the lactic acid bacterium in step (2) is 10 ⁵ to 10 ⁶ cfu / ml at the initial stage of inoculation, or a composition for preventing or ameliorating the skin hypersensitivity reaction by the laser for the cosmetic purpose. 10. The method of claim 9,
Wherein the alcohol solvent in step (3) is 20 to 90 wt% ethanol aqueous solution, and the extraction in step (3) is performed using the aqueous ethanol solution for 12 to 72 hours. For preventing or ameliorating a skin hypersensitivity reaction. 10. The method of claim 9,
The alcohol solvent extract filtered in the step (4) is concentrated in vacuo at a temperature of 40 to 80 ° C and then dried by vacuum drying, spray drying or freeze drying. A composition for preventing or ameliorating a skin hypersensitivity reaction by a laser, which is performed for the treatment of skin lesions or for cosmetic purposes. A cosmetic formulation for preventing or ameliorating skin irritation caused by laser treatment for the treatment of skin lesions or for cosmetic purposes, characterized by containing an onion fermented extract. 19. The method of claim 18,
Wherein the cosmetic composition is at least one selected from the group consisting of a lotion, a lotion, a cream, a lotion, an essence, a pack, a gel or a mist. 20. The method of claim 19,
But are not limited to, glycerin, butylene glycol, dipropylene glycol, carboxyvinylpolymer, polysorbate 80, hydrogenated caster oil, glyceryl stearate glyceryl stearate, glyceryl stearate, cetearyl alcohol, cetyl ethylhexanoate, glycyrrhiza inflate extract, and lecithin. A cosmetic formulation for preventing or ameliorating skin irritation caused by laser treatment for lesion treatment or for cosmetic purposes. 21. The method of claim 20,
To 100 parts by weight of an onion fermented extract, 500 to 700 parts by weight of glycerin; 900 to 1100 parts by weight of butylene glycol; 1100 to 1300 parts by weight of dipropylene glycol; 50 to 70 parts by weight of a carboxyvinylpolymer; 90 to 110 parts by weight of polysorbate 80; 700 to 900 parts by weight hydrogenated caster oil; 90 to 110 parts by weight of glyceryl stearate; 180-220 parts by weight of cetearyl alcohol; 500 to 700 parts by weight of cetyl ethylhexanoate; 10 to 30 parts by weight of glycyrrhiza inflate extract; And 10 to 30 parts by weight of lecithin. The cosmetic preparation for preventing or ameliorating the skin irritation caused by laser treatment for the treatment of skin lesions or for cosmetic purposes.

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