KR20170092293A

KR20170092293A - Cosmetic Composition Containing Fermented Extract from Lycium Chinense and Manufacturing Method Thereof

Info

Publication number: KR20170092293A
Application number: KR1020160013453A
Authority: KR
Inventors: 구태규; 박안나; 박홍순
Original assignee: 주식회사 에코바이오의학연구소
Priority date: 2016-02-03
Filing date: 2016-02-03
Publication date: 2017-08-11

Abstract

The present invention relates to a cosmetic composition having a whitening and antibacterial effect by using a fermented extract of Gugija leaves, fruits and roots, and a method of producing the same. The cosmetic composition of the present invention comprises a gugija powder obtained by drying Gugija leaves, The fermented gugija powder is inoculated with the yeast culture solution at a concentration of 1 to 20% by weight and the fermented gugija powder is fermented at 25 to 35 ° C for 72 to 96 hours. Deg.] C, for 12 to 24 hours at a low temperature extraction method.
According to the above-described constitution, the present invention can provide a cosmetic composition which can improve not only the whitening effect but also the antibacterial effect and the skin troubles such as atopic dermatitis by the useful components of the ginger.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition comprising a fermented extract of Gujija and a method for producing the same,

The present invention relates to a cosmetic composition comprising a fermented extract of Gujuga extract and a method for producing the same, and more particularly to a cosmetic composition containing a fermented extract obtained by fermenting Gujuga leaf, fruit and root with yeast, To provide a cosmetic composition for improving atopic dermatitis and the like by having antimicrobial properties against Staphylococcus aureus, which produces a toxic substance acting as a superantigen on the skin surface of a pest of a human body, in particular, &Lt; / RTI >

In recent years, functional materials using natural materials have been actively developed, and functional materials using herbal medicines known to contain useful components have been actively developed.

Lycium Chinensis Miller (Lycium Chinensis Miller) is a deciduous shrub belonging to the genus Chrysanthemum. It is originated from Asia Minor.

Gugija refers to the fruit of Lycii fructus. The bark of the root is called the fusiformis, the leaf is the fusiform leaf, and the lips are called the puschos. According to Donguibogam, Gojija is effective for Nutrition, Tonic, Blood, and Zigal. According to Gangbuk, Gugija has no toxicity, it does not burn in heat and cold, it does not burn in heat and cold, it is diabetic with chest inflammation and thirst It is said that the nerve is good for the paralyzed disease, and the lungs and the kidney function are promoted to improve the vision.

Gugija contains various functional ingredients such as betaine, cholin, physalien, rutin, β-sitosterol and zeazanthin. It has various physiological activities such as antioxidant effect, antibacterial and anticancer effect, immunity enhancement effect, liver function improvement effect, blood cholesterol lowering effect .

The skin color of the human body is generally determined by melanin, redox hemoglobin, carotene and melanoid, and melanin is the biggest influence. Melanin is made from melanocytes and protects the skin from harmful free radicals generated from sunlight, especially ultraviolet light. After melanin is formed, the original skin color is restored by skin metabolism in healthy skin, but in aged skin, melanin pigment does not fall off from skin with dead keratin, and it is deposited on skin and becomes stain. Therefore, in order to maintain skin health, excessive synthesis of melanin should be suppressed.

Melanin is formed by tyrosine as a substrate of tyrosine in skin cells. It is formed by enzymes through intermediary metabolites of dopa and dopachrome. As a method for inhibiting the synthesis of melanin, a cosmetic containing a suitable ultraviolet screening agent may be used to prevent or reduce exposure to ultraviolet rays, to inhibit the activity of tyrosinase to inhibit melanin synthesis, or to inhibit the melanin synthesis, And the like. However, currently used substances such as albutin, 5-hydroxy-2-hydromethyl-v-pyrone and hydroquinone show low inhibitory activity, instability of the substance itself and toxicity to skin I have a problem.

Therefore, a natural inhibitor having no toxicity to skin and stable and having a high inhibitory effect on melanin production is required, and in recent years, interest in the usefulness of natural materials has increased, and herbal medicine herbal medicine for skin care has been widely used.

The oxygen that the creature breathe has the lowest energy level and is the most stable form when there are two electrons in the outermost orbital. This type of oxygen is called triplet oxygen. When the triplet oxygen absorbs a large amount of energy by heat energy, light energy, chemical energy, etc., the orbital of the outermost orbitals is removed by the reduction reaction, so that the outermost orbitals are converted into singly oxygenated singlet oxygen, Of oxygen is referred to as active oxygen.

The active oxygen requires electrons to fill the electron, because the outermost orbital is not removed, and it reacts easily with the electron present and accelerates the oxidation reaction very rapidly.

As a common example, in skin cells exposed to ultraviolet rays, strong light energy converts triplet oxygen into harmful oxygen singlet oxygen, which rapidly oxidizes the cells, resulting in rapid aging of the skin.

Superoxide dismutase (SOD), catalase, glutathione peroxydase, vitamin E and the like exist as a catalyst for the mechanism of preventing the oxidation of cell bodies from free radicals in the body. Since antioxidants gradually decrease their ability to defend against active oxygen as they get older, various diseases come and rapidly aging. Therefore, it is required to develop a natural antioxidant having a strong antioxidant ability without any harm to human body.

In addition to skin aging caused by active oxygen, skin troubles that appear in various forms are fatal to the members of modern society seeking external beauty. Among the causes of such skin troubles, It is often a parasitic microorganism. Among the various microorganisms, the toxin secreted by Staphylococcus aureus acts as a superantigen, which causes a problem of atopic dermatitis on the skin surface.

Since the chemical antimicrobials applied on the skin surface cause strong skin irritation irrespective of the effect, it is necessary to develop a natural antimicrobial agent having no skin irritation because it becomes burden to use in a state having various troublesome skin diseases, Development of natural antimicrobial agents against Staphylococcus aureus, which is related to diseases, is required.

On the other hand, the fermented extract of Gujusima contains 18.1% by weight of crude protein, 11.2% by weight of crude fiber, 12.39% by weight of free amino acid, 6.2% by weight of free sugar, 633.1% by weight of tannin and 1877.5% by weight of organic acid, These components show antibacterial activity and are used as a main component of natural antimicrobial agents.

In addition, when these natural materials are fermented, antioxidant activity tends to increase not only in antimicrobial activity but also in polyphenol content as a whole. Experimental results showing that antioxidative activity of garlic is increased in case of garlic fermented.

KR 10-2013-0019186 A

SUMMARY OF THE INVENTION Accordingly, the present invention has been made in order to solve the problems of the conventional cosmetic composition as described above. The present invention provides an antimicrobial effect And thus has a whitening and antibacterial effect, and a method for producing the same.

It is an object of the present invention to provide a cosmetic composition comprising the above-described fermented extract of Gujuga, wherein the cosmetic composition is prepared by pulverizing the gugija leaf, the fruit and the root by pulverizing the gugija powder, %, Fermented at 25 to 35 ° C for 72 to 96 hours, and then subjected to low-temperature extraction for 12 to 24 hours at an extraction temperature of 20 to 60 ° C with water having a weight of 1 to 10 times the weight of the fermented ginger powder And the fermented extract obtained by repeatedly obtaining the fermented sea mustard.

Another object of the present invention is to provide a method for producing a cosmetic composition comprising a Ganoderma lucidum extract, comprising the steps of: drying and pulverizing gypsum raw material comprising leaves, fruits and roots of Gugija; A yeast culture step of inoculating and cultivating gypsum powder obtained by the step of drying and pulverizing a yeast culture solution of 1 to 20 wt% based on the gypsum powder; A fermentation step of fermenting the koji powder containing the yeast cultured by the yeast culture step at 25 to 35 DEG C for 72 to 96 hours; Extracting the fermented extract by repeating a low temperature extraction method at a temperature of 20 to 60 ° C for 12 to 24 hours using 1 to 10 times the weight of water as a solvent in the ginger powder having been fermented by the fermentation step And; And a step of preparing a cosmetic composition comprising 0.01 to 10% by weight of a fermented extract obtained by extracting the fermented extract.

According to the present invention, the fermentation extract of Gujuga fermentation extracted by the fermentation extract step is removed by using a centrifugal separator, and the yeast remaining in the extract is completely removed by passing through a fine filter having an eye size of 2 μm, The fermented extract is obtained or lyophilized to obtain a fermented ginger extract in powder form.

The present invention can prevent or improve skin troubles such as atopic dermatitis and the like while having an excellent antibacterial effect as well as a whitening effect by forming a cosmetic composition by using a fermented sea mustard extract extracted from the leaves, fruits and roots of Gugija.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a process chart showing a method of manufacturing a cosmetic composition comprising a fermented extract of Gujuga gigas according to the present invention,
FIG. 2 is a graph showing the inhibitory effect of tyrosinase activity in the fermented Gujuga extract according to the present invention.

Hereinafter, the structure and operation of the present invention will be described in more detail with reference to the accompanying drawings, which show preferred embodiments.

The present invention relates to a cosmetic composition comprising a fermented extract of Ganoderma lucidum having an antibacterial effect against Staphylococcus aureus which secretes toxins which function as a superantigen, which is responsible for reducing melanin and causing skin troubles, and a process for producing the same. The constitution of the cosmetic composition and the manufacturing method will be explained together by explaining the method of producing the cosmetic composition.

The method of manufacturing a cosmetic composition according to the present invention comprises the steps of drying and pulverizing (S100), yeast culture (S200), fermentation (S300), fermentation extract extraction (S400) ).

(1) drying and pulverization step (S100)

This step is a step of drying and crushing a raw material of a ginger comprising leaves, fruits and roots of a ginger, and in the present invention, the prepared ginger raw material is first cleaned with water to remove foreign substances and then sterilized by high pressure to remove germs , Germinating raw material free from germs is allowed to dry naturally in the shade for a certain period of time, and then the dried raw germination material is put into a grinder and finely pulverized to obtain a ginger powder.

(2) Yeast culture step (S200)

This step is a step for culturing the yeast (Yarrowia lipolytica) culture solution by adding the yeast (Yarrowia lipolytica) to the yeast powder after the yeast powder is obtained in the above drying and pulverization step (S100). In this step, 1 to 20 wt% yeast culture Lt; / RTI >

(3) Fermentation step (S300)

This step is a step of fermenting the gugija powder containing the cultured yeast when the yeast is cultured in the gugija powder by the above yeast culture step (S200), wherein the fermentation is carried out at a temperature of about 25-35 < 0 & Time.

(4) fermentation extract extraction step (S400)

This step is a step of obtaining a Gujuza fermentation extract from the ginger powder which has been fermented by the above fermentation step (S300) through a low temperature extraction method. For this purpose, in the present invention, the ginger powder is fermented at 1 to 10 times The fermented extract is extracted by low temperature extraction method by repeating 3 times for 12 to 24 hours at a temperature of 20 ~ 60 캜 using a weight of water as a solvent. When the fermented extract is extracted by the low temperature extraction method, the fermented extract of Gujuga fermentation is centrifuged And then the remaining suspension is removed. Then, the remaining yeast in the extract is completely removed by passing through a fine filter having an eye size of 2 탆 to prepare a liquid fermentation extract or lyophilized to obtain a powdery goji fermentation An extract is obtained.

(5) Cosmetic composition preparing step (S500)

This step is a step of preparing a cosmetic composition composed of a formulation such as softened longevity, nutritional cream, emulsion, essence and the like using the fermented extract of Gujuga extract obtained in the fermentation extract extraction step (S400) When the composition is prepared, the cosmetic material contains about 0.01 to 10% by weight of the fermented extract of Gujuga.

In order to confirm whether the cosmetic composition prepared according to the method of manufacturing a cosmetic composition of the present invention has a function of improving the skin whitening function and skin disease by effectively expressing the above-mentioned composition, , A fruit and root were used to obtain a roasted fermented extract, and then several kinds of cosmetic formulations containing the fermented extract were prepared, and their performance was tested using them. Explain.

end. Example 1 (Preparation of softening agent)

In Example 1, as shown in Table 1 below, a softening water containing 10% by weight of the fermented sea urchin extract was prepared based on the total weight of the cosmetic composition.

Ingredients Content (% by weight) Fermented extract 10.0 ethanol 5.0 glycerin 2.0 1,3-butylene glycol 5.0 Sodium hyaluronate 2.0 Disodium edetate 0.01 Preservative, coloring, fragrance 0.01 to 1.0 Purified water Balance

I. Example 2 (Preparation of nutritional cream)

In Example 2, as shown in Table 2 below, a nutritional cream containing 5% by weight of fermented sea urchin extract was prepared based on the total weight of the cosmetic composition.

Ingredients Content (% by weight) Fermented extract 5.0 glycerin 3.0 1,3-butylene glycol 5.0 Cetyl alcohol 3.0 Sorbitan stearate 1.0 Stearic acid 1.0 Polysorbate 60 2.0 Triethanolamine 0.3 Grape seed oil 3.0 Preservative, coloring, fragrance 0.01 to 1.0 Purified water Balance

All. Example 3 (Preparation of emulsion)

In Example 3, as shown in Table 3 below, an emulsion containing 5% by weight of a fermented extract of Gujuga extract was prepared based on the total weight of the cosmetic composition.

Ingredients Content (% by weight) Fermented extract 5.0 glycerin 3.0 1,3-butylene glycol 5.0 Cetyl alcohol 2.0 Sorbitan stearate 1.0 Stearic acid 1.0 Polysorbate 60 2.0 Triethanolamine 0.3 Olive oil 2.0 Preservative, coloring, fragrance 0.01 to 1.0 Purified water Balance

la. Example 4 (Preparation of essence)

In Example 4, as shown in Table 4 below, an essence containing 7% by weight of the fermented extract of Gujuguaca relative to the total weight of the cosmetic composition was prepared.

Ingredients Content (% by weight) Fermented extract 7.0 glycerin 5.0 1,3-butylene glycol 7.0 ethanol 4.0 Polyoxyethylene alcohol ether 1.0 Sodium hyaluronate 5.0 Grape seed oil 2.0 Camellia oil 1.0 Preservative, coloring, fragrance 0.01 to 1.0 Purified water Balance

a. EXPERIMENTAL EXAMPLE 1 (Experiment of Whitening Effect of Fermented Extract of Gujuga)

In Experimental Example 1, a mouse derived B-16 melanoma (B16 BL6) cell line (KCLB 80006) was purchased and a 10% FCS fetal calf serum (CO 2 concentration: 5%) at 37 ° C for 24 hours, and 28.8 μl of the fermented extract of Gujusma was treated in the cultured melanoma cell culture medium. After culturing for 5 days, the cultured cultures were washed with PBS (phosphate buffered in saline), treated with 0.05% trypsin and 0.53 mM EDTA solution to separate adsorbed cells, and then centrifuged for 5 minutes to obtain cells.

Then, the number of cells was measured, the number of cells in each experimental group was kept constant, and then centrifuged again to obtain precipitated melanin. Melanin was dissolved by treating with 1N NaOH and absorbance was measured at 405 nm. At this time, as a control, the cells not treated with the extract were compared with the amount of melanin in the control, and the inhibitory effect of the extract on melanin synthesis was measured. The amount of melanin was determined by using Hosoi's method, and cells were seeded at 6 × 10 5 cells / well in 6 well plates. The samples were treated for 48 hours at 37 ° C. in a CO2 incubator Respectively.

Cells were collected by centrifugation at 1,200 rpm for 5 min and then resuspended in 1 ml homogenization buffer (50 mM sodium phosphate pH 6.5, 1% Triton X-100, 2 mM PMSF) .

To the pellet obtained above, 200 μl of 1 N NaOH (10% DMSO) was added and stirred. The OD 405 value was measured at 405 nm. Using a standard calibration curve obtained with a melanin standard (Sigma, USA) well) was calculated. Melanin was compared with the amount of melanin produced in the unit cells (104 cells). As a result, the amount of melanin produced was inhibited. As a result, it was found that the Gugija extract according to the present invention had a whitening effect.

As a result, it was confirmed that melanin synthesis was inhibited by the cell culture medium containing the fermented extract of Gujuga extract. Therefore, when the cosmetic composition containing the fermented extract of Gujuga extract is used for a long time, it has no skin toxicity and is safe and has a high inhibitory effect on melanin production. Effect can be expected.

b. Experimental Example 2 (Measurement of intracellular tyrosinase activity of Gujuga fermentation extract)

Experimental example 2 was an experiment for measuring the activity of intracellular tyrosinase in the fermented extract of Gujugi, using the method of Pawelk and Pomerantz for the measurement of tyrosinase activity in cells. Cells were seeded at 5 × 10 5 cells / well in a 6-well plate And the cells were collected and dissolved in the sample. Then, 0.1 M sodium phosphate buffer (pH 6.8) supplemented with 0.2% L-DOPA was added thereto and incubated at 37 ° C for 2 hours and absorbance at 490 nm Were measured

To examine the inhibitory effect of tyrosinase activity of the compositions, 0.4 mL of 10 mM L-DOPA (L-dihydroxyphenylalanine) dissolved in 0.1 M sodium phosphate buffer (pH 6.8) as a substrate of tyrosinase was added to 0.2 mL After incubation at 25 ° C for 15 minutes, the reaction was stopped in the freezing condition, and the absorbance was measured at 475 nm. After the addition, 0.4 mL of tyrosinase (100 units / mL) was added to the mixed solution at room temperature.

In the control group, distilled water was used instead of the extract, and the inhibitory effect of tyrosinase was calculated using the following equation (1).

here,

(%) Of tyrosinase activity inhibition,

The absorbance of the extract,

Is the absorbance of the control group.

FIG. 2 is a graph showing the results of the above experiment. As a result, it was found that the tyrosinase inhibition rate was greatly increased by including the fermented extract of Gujuga fulbifolia. In FIG. 2, a control group which is generally known to have high tyrosinase activity inhibition rate Arbutin), and Gugija fermented extracts were prepared in accordance with the concentrations of 25, 50, and 100, and compared with the control (control) in which both the control and gugija fermented extracts were not used From the results, it can be seen that the fermented extract having a concentration of 100 gigajoules had an efficacy comparable to the inhibitory activity of the tyrosinase activity of the control group.

c. Experimental Example 3 (Antimicrobial effect test of Gujuga fermented extract)

In Experimental Example 3, a minimal inhibition concentration (MIC) assay was conducted to examine the antimicrobial activity of Staphylococcus aureus extracts against Staphylococcus aureus.

For this purpose, a fermented extract of Gujia mugwort was added at a constant concentration interval from 0.01 to 0.5% by weight in a BHI medium, which is a culture medium of Staphylococcus aureus, and solidified. Then, a predetermined amount of the pre-cultured Staphylococcus aureus was added to the solid medium After inoculation, the cells were cultured in an incubator fixed at 37 ° C for 24 hours to observe the growth of the bacteria.

In order to compare the degree of actual antibacterial activity, triclosan and salicylic acid, which are widely used in antimicrobial-related cosmetics due to their wide range of antimicrobial activity, were added to the medium at the same concentration, The results are shown in Table 5.

Sample concentration (% by weight) 0.01 0.05 0.10 0.15 0.20 0.25 0.30 0.35 0.40 0.50 Fermented extract + + + + - - - - - - Triclosan + + - - - - - - - - Salicylic acid + + + - - - - - - -

Here, + indicates no antimicrobial activity, and - indicates antimicrobial activity.

As shown in Table 5, the fermented extract of Gujugi japonica showed the antimicrobial activity against Staphylococcus aureus at 0.20% by weight and showed a similar level to that of triclosan or salicylic acid showing antibacterial activity at 0.10% by weight and 0.15% by weight .

d. Experimental Example 4 (Antimicrobial Effect Test of Cosmetic Composition Containing Fermented Extract of Gujuga)

In Experimental Example 4, the antimicrobial activity test was carried out in the same manner as in Experimental Example 3, using the cosmetic compositions of Formulations 1 to 4 prepared using the fermented extract of Gujuga falciparum of the present invention as a sample.

Sample concentration (% by weight) 0.01 0.05 0.10 0.15 0.20 0.25 0.30 0.35 0.40 0.50 Example 1 + + + + + + - - - - Example 2 + + + + + - - - - - Example 3 + + + + + + - - - - Example 4 + + + + + - - - - -

Experimental Results As shown in Table 6, it was confirmed that the cosmetic compositions of Examples 1 to 4 exhibited antibacterial activity at 0.30 wt% or more.

INDUSTRIAL APPLICABILITY As described above, the present invention relates to a cosmetic composition which can prevent or treat skin troubles such as atopic dermatitis and the like while having an excellent antibacterial effect as well as a whitening effect by using a fermented extract of Gujuga leaf, fruit and roots extracted from roots, Composition can be provided.

Claims

The gugija powder pulverized by drying the gugija leaves, fruits and roots was inoculated with 1 ~ 20% yeast culture liquid to the weight of the gugija powder, fermented at 25 ~ 35 ℃ for 72 ~ 96 hours, Which comprises extracting a fermented sea mustard extract obtained by repeating low temperature extraction for 12 to 24 hours at an extraction temperature of 20 to 60 DEG C with water having a weight of ~ 10 times as a solvent. .

A drying and grinding step (S100) for drying and pulverizing the raw material comprising the leaves, fruits and roots of the gargoyle;
A yeast culture step (S200) in which a yeast culture solution is inoculated and cultured in a gypsum powder obtained by the drying and pulverization step (S100) in an amount of 1 to 20% by weight based on the gypsum powder;
A fermentation step (S300) of fermenting the gugija powder containing the yeast cultured by the yeast cultivation step (S200) at 25 to 35 DEG C for 72 to 96 hours;
The fermentation extract is repeatedly obtained by low temperature extraction at a temperature of 20 to 60 ° C for 12 to 24 hours using 1 to 10 times the weight of water as a solvent in the ginger powder having been fermented by the fermentation step (S300) Fermentation extract extraction step (S400);
And a cosmetic composition preparation step (S500) of producing a cosmetic composition containing 0.01 to 10% by weight of the fermented extract of Gujusma extract obtained by extracting the fermentation extract (S400) (S500). Gt;

The method of claim 2,
The fermented extract of Gujuga extract extracted by the fermentation extract extraction step (S400) is removed by using a centrifugal separator, and the yeast remaining in the extract is completely removed by passing through a fine filter having an eye size of 2 μm, A method for producing a cosmetic composition comprising a fermented extract of Gujuga fermentation, which comprises obtaining a fermented extract or a lyophilized powder in a powder state by lyophilization.