KR20010027448A - Cosmetic compositions - Google Patents

Abstract

PURPOSE: Notoginseng Radix extracts, Cucumis melo extract, Dendrobii Herba extracts, and a cosmetic composition containing more than one of extracts selected therefrom are provided, which show very strong melanogenic inhibiting effect, and gives skin-whitening effect and freckles-improvement effect without side effects when applied on human skin. CONSTITUTION: A process for the preparation of Notoginseng Radix, Cucumis melo, Dendrobii Herba extracts is comprised of: chopping Notoginseng Radix, Cucumis melo, Dendrobii Herba as fine as possible, and extracting in an extractor at 50-100deg.C for 1-5hrs with primary alcohol, ethyl acetate or acetone; filtering and extracting the residue with same process for more than one time; concentrating the extract under decompression, and freeze-drying or spray-drying to get dried extract. The cosmetic composition contains Notoginseng Radix, Cucumis melo and Dendrobii Herba extract 0.0001-10.0wt., desirably 0.0005-10.0wt., which can be prepared in the form of ointments for skin, or cosmetics such as lotions.

Description

Cosmetic composition containing Samchil extract, berry extract, Seokgok extract and at least one selected from them {Cosmetic compositions}

The present invention relates to a cosmetic composition containing three or more extracts selected from samchil extract, nectar extract, and Seokgok extract. More specifically, the present invention relates to a cosmetic composition containing three or more kinds of samil extracts, nectar extracts, stone grain extracts excellent in melanin production inhibitory effect.

It is everyone's constant desire to have white, fair skin. Human skin color is genetically determined by the concentration and distribution of melanin in the skin, but is also influenced by environmental or physiological conditions such as solar ultraviolet rays, fatigue and stress. Melanin is a type of amino acid tyrosine (tyrosinase) acts as an enzyme called tyrosinase (tyrosinase) is converted to dopa (DOPA), dopaquinone (dopaquinone) is produced through a non-enzymatic oxidation reaction. However, although the pathway by which melanin is made is known, it is still not clear what is the mechanism that induces melanin synthesis, the previous step in which tyrosinase works. On the other hand, by inhibiting the synthesis of melanin pigment, not only can the skin tone be brightened to realize the skin whitening, but also the improvement of skin hyperpigmentation such as blemishes caused by ultraviolet rays, hormones, and heredity, ultraviolet rays, and freckles caused by heredity. . Therefore, conventionally, a substance having an inhibitory activity against tyrosinase such as hydroquinone, ascorbic acid, kojic acid, glutathione, and the like is combined with an ointment or a cosmetic, and the skin is whitened or stained. Although it has been used for the purpose of improving freckles, the whitening effect of hydroquinone is recognized, but the amount of use is extremely limited due to severe skin irritation. In addition, ascorbic acid is easily oxidized, and cosmetics containing the same cause problems such as discoloration and discoloration. Thiol compounds such as glutathione and cysteine have a characteristic unpleasant odor and have problems with transdermal absorption. In addition, glycosides and derivatives thereof have a high polarity and thus have problems in being used as a component of cosmetics. In addition, placenta extract has a problem that the whitening effect is insufficient.

The present invention has been made to solve the above problems, the present inventors can use the natural melanoma cells (B16 mouse melanoma cells) that can screen (g) screening to the substance that inhibits the melanin synthesis induction itself In order to develop a new whitening agent for native plants and plants, the results of repeated studies, it was found to have a very powerful melanin production inhibitory effect and whitening effect in the three-six extract, nectar extract, Seokgok extract to complete the present invention. Accordingly, it is an object of the present invention to provide a cosmetic composition containing three or more extracts selected from three kinds of extracts, nectar extracts, grains and extracts thereof.

Hereinafter, the configuration and operation of the present invention will be described in detail so that those skilled in the art can easily perform.

Samchil is a perennial herb and has been used for the treatment of various blood disorders, vaginal pain, and cardiac traffic as the roots of the panax pseudoginseng and P. notoginseng. In addition, ginsenoside Rg1 has been reported to have anti-cancer effects in the ginseng component (Food Factors Cancer Prev., (Int. Conf.) (1997), 235-239). Cucurbitaceae belong to Cucurbitaceae, scientific name is Cucumis melo, fruit, stem, leaf, root, seed, flower, etc. are used for fish blood and menstrual excess (Medicinal Chinese Dictionary). In the past, there have been reports of compounds such as β-carotene, cry ptoxanthine, meloside A, and L. Seokgok is a perennial herb and includes the orchid family Dendrobium nobile, D. lina wianum, D. officinale, D. moniliforme, D. hercoglossum, and has been used as a medicinal herb to produce essences, lower heat, and nourish yin. (Chinese medicine dictionary).

In the present invention, Samchil, Chuk, Seokgok, which can be purchased as a medicine, is first extracted with an organic solvent such as anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms or ethyl acetate or acetone, and then concentrated under reduced pressure, and then lyophilized or spray dried. Tea extract was prepared. Samchil, Chuk, and Seokgok extracts prepared in this way show very potent melanin synthesis inhibitory effects in B-16 mou se melanoma cells. In addition, the skin whitening ointment or cosmetics containing dried samchil, cherries, and granule extract are applied to the skin of a person, the skin whitening effect is very excellent.

The present invention will be described in detail as follows.

Shamil, cucurbits, and grains, available as medicinal herbs, are pulverized and refluxed with 5-20 volumes of water or 1-4 anhydrous or hydrous lower alcohols, ethyl acetate or acetone, based on the dry weight of the crushed powder. Extract by heating 1-5 hours at 50-100 ° C. in the extractor. After filtering with a filter cloth, the residue is extracted one more time in the same manner. The extracts were combined and concentrated under reduced pressure, followed by freeze drying or spray drying to obtain a dry extract. When dried Samchil, Chuk, and Seokgok extracts were treated with B-16 mouse melanoma cells, they showed very potent melanin synthesis inhibitory effects. Various cosmetic preparations such as skin external ointments and lotions containing Samchil, Chuk, and Seokgok extracts obtained as described above are prepared. The skin whitening ointment or lotion containing Samchil, Chukwa, and Seokgok extracts was prepared and applied to human skin. In addition, it can be seen that no side effects occur Shamilchi, icicles, grains extract is safe and very effective blemishes and freckles and skin whitening agent.

The amount of Samchil, Chuk, and Grain extracts included in the preparation of external skin ointments or cosmetics is appropriately 0.0001-10.0 weight, preferably 0.0005-10.0 weight in dry weight. A clear effect could not be expected at concentrations of less than 0.0001 weight, and no apparent effect increased with increasing content at concentrations above 10 weight.

Next, the present invention will be described in detail with reference to Examples, and the present invention is not limited to the following Examples.

Example 1-18

100 g of crushed pulverized powder obtained by purchasing commercially available samchil, cherries, and grains is placed in 3L of a solvent such as water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, or acetone, and 3 at a reflux extractor with a cooling capacitor. The mixture was boiled for time, extracted with 100 mesh filter cloth, and the residue was extracted once more in the same manner. Each extract was combined and filtered using Whatman No. 2 filter paper at room temperature to remove insoluble materials, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser and then lyophilized to dry weight as shown in Table 1 below. Got.

Dry weight of extractant and extract Three Cucurbit Seokgok 80 Methanol 26 g (Example 1) 14 g (Example 7) 11 g (Example 13) Purified water 24 g (Example 2) 15 g (Example 8) 13 g (Example 14) 85 Ethanol 27 g (Example 3) 14 g (Example 9) 13 g (Example 15) 70 acetone 16 g (Example 4) 18 g (Example 10) 9 g (Example 16) Ethyl Acetate 15 g (Example 5) 13 g (Example 11) 10 g (Example 17) Saturated normal butanol 20 g (Example 6) 12 g (Example 12) 12 g (Example 18)

Experimental Example 1. Whitening effect at the cellular level

Each of the extracts obtained according to Examples 1-18 was added to the culture solution of mouse B-16 mouse melanoma cells to test the whitening effect at the cellular level (Lotan R., Lotan D. Cancer Res. 40: 3345-3350, 1980). Each extract obtained according to Example 1-18 was added to a culture medium of B-16 melanoma cells with a hydroquinone aqueous solution in a final concentration of 0.005 and then cultured for 3 days, followed by trypsin treatment. Melanin was extracted after centrifugation off the vessel. To this, 1 ml of sodium hydroxide solution (1N concentration) was added, boiled for 10 minutes to dissolve the melanin, and the absorbance was measured at 400 nanometers (nm) using a spectrophotometer to determine the amount of melanin produced per unit cell number (10 ⁶ cells). It was performed by the method shown as, the relative melanin production relative to the control was calculated as the inhibition rate (), the results are summarized in Table 2.

Inhibitory Effects of Samchil Extract, Peak Extract, and Seokgok Extract at Cell Level sample Melanin production Inhibition rate () Control group (no addition) 0.045 ± 0.004 - Example 1 (final concentration 0.005) 0.030 ± 0.002 33 Example 2 (final concentration 0.005) 0.030 ± 0.003 33 Example 3 (final concentration 0.005) 0.027 ± 0.002 40 Example 4 (final concentration 0.005) 0.025 ± 0.002 44 Example 5 (final concentration 0.005) 0.024 ± 0.003 47 Example 6 (final concentration 0.005) 0.026 ± 0.003 42 Example 7 (final concentration 0.005) 0.036 ± 0.004 20 Example 8 (final concentration 0.005) 0.037 ± 0.002 18 Example 9 (final concentration 0.005) 0.032 ± 0.002 29 Example 10 (final concentration 0.005) 0.030 ± 0.002 33 Example 11 (final concentration 0.005) 0.028 ± 0.002 38 Example 12 (final concentration 0.005) 0.024 ± 0.002 47 Example 13 (final concentration 0.005) 0.027 ± 0.003 40 Example 14 (final concentration 0.005) 0.030 ± 0.004 33 Example 15 (final concentration 0.005) 0.028 ± 0.002 38 Example 16 (final concentration 0.005) 0.023 ± 0.002 49 Example 17 (final concentration 0.005) 0.021 ± 0.002 53 Example 18 (final concentration 0.005) 0.022 ± 0.002 51 Hydroquinone (final concentration 0.0001) 0.025 ± 0.002 44 * Repeat = 3

As can be seen from the results of Table 2, considering that each sample obtained according to Examples 1-18 is an extract that is not purified at all, compared to the hydroquinone which is a known whitening substance, it is very good for the melanoma cells of cultured mice. It can be seen that there is a strong melanin production inhibitory ability.

Experimental Example 2. Investigation of melanin production inhibitory ability

The melanoma production inhibitory ability of the rat melanoma cells cultured in the same manner as in Experimental Example 1 was examined according to the concentration of the extracts of Examples 1, 7, and 13, and the results are shown in Table 3 below.

Inhibitory effect of melanogenesis at the cellular level sample Application concentration () Melanin production Inhibition rate () Control group (no addition) - 0.045 ± 0.004 - Example 1 Final concentration (0.0005) 0.037 ± 0.002 18 Final concentration (0.005) 0.030 ± 0.002 33 Final concentration (0.05) 0.025 ± 0.002 44 Concentration (0.5) 0.019 ± 0.001 58 Example 7 Final concentration (0.0005) 0.040 ± 0.002 11 Final concentration (0.005) 0.036 ± 0.004 20 Final concentration (0.05) 0.025 ± 0.002 44 Concentration (0.5) 0.020 ± 0.001 56 Example 13 Final concentration (0.0005) 0.035 ± 0.002 22 Final concentration (0.005) 0.027 ± 0.003 40 Final concentration (0.05) 0.016 ± 0.002 64 Concentration (0.5) 0.015 ± 0.001 67 Hydroquinone (0.0001) 0.025 ± 0.002 44 Repeat = 3

As can be seen in the results of Table 3, Samchil extract, the peak extract, and Seokgok extract can be seen that it has a very strong melanin production inhibitory ability against the cultured melanoma cells. Hydroquinone has a strong melanin production inhibitory ability at low concentrations, but the cytotoxicity can not be tested at more than 0.0001, whereas the extracts of the present invention is more than the maximum amount of hydroquinone available without showing cytotoxicity even at 0.05 concentration or more It has a high melanin production inhibitory effect. In addition, Shamil extract, fructose extract, and Seokgok extract have very high melanin production inhibitory ability, considering that it is not a single substance but a mixture.

It can be very useful for improvement or skin whitening.

Hereinafter, the formulation example of the cosmetics containing Samchil, Chuk, and Seokgok extract is shown by the manufacture example.

Preparation Example 1-3 and Comparative Example 1

Examples of prescription external skin ointments containing Samchil, Chuk, and Seokgok Extract are as follows. Samchil, Chuk, Seokgok extract refers to those of Examples 5, 11 and 17, respectively.

Composition Production example (weight) Comparative Example 1 One 2 3 -extract Ginseng extract 10 - - - Icicle extract - 10 - - Seokgok Extract - - 10 - Diethyl Sebacate 8 8 8 8 -Payment 5 5 5 5 Polyoxyethylene oleyl ether phosphate 6 6 6 6 Sodium benzoate Quantity Quantity Quantity Quantity -vaseline to 100 to 100 to 100 to 100

Preparation Example 4-6 and Comparative Example 2

A prescription example of cream in cosmetics containing Samchil, Chuk, and Seokgok extract is as follows. Samchil, Chuk, Seokgok extract refers to those of Examples 5, 11 and 17, respectively.

Composition Production example (weight) Comparative Example 2 4 5 6 -extract Ginseng extract 1.0 - - - Icicle extract - 1.0 - - Seokgok Extract - - 1.0 - Stearic acid 15.0 15.0 15.0 15.0 Cetanol 1.0 1.0 1.0 1.0 Potassium hydroxide 0.7 0.7 0.7 0.7 -glycerin 5.0 5.0 5.0 5.0 Propylene glycol 3.0 3.0 3.0 3.0 -antiseptic Quantity Quantity Quantity Quantity -incense Quantity Quantity Quantity Quantity -Purified water to 100 to 100 to 100 to 100

Preparation Example 7-9 and Comparative Example 3

Prescription examples of supple cosmetics in cosmetics containing Samchil, Chuk, and Seokgok extract are as follows. Samchil, Chuk, Seokgok extract refers to those of Examples 5, 11 and 17, respectively.

Composition Production example (weight) Comparative Example 3 7 8 9 -extract Ginseng extract 0.2 - - - Icicle extract - 0.2 - - Seokgok Extract - - 0.2 - -ethanol 10.0 10.0 10.0 10.0 Polylauric acid polyoxyethylene sorbitan 1.0 1.0 1.0 1.0 Paraoxybenzoic acid methyl 0.2 0.2 0.2 0.2 -glycerin 5.0 5.0 5.0 5.0 -1,3-butylene glycol 6.0 6.0 6.0 6.0 -incense Quantity Quantity Quantity Quantity Pigment Quantity Quantity Quantity Quantity -Purified water to 100 to 100 to 100 to 100

Preparation Example 10-12 and Comparative Example 4

Prescription examples of nutrient cosmetics in cosmetics containing Samchil, Chuk, and Seokgok extract are as follows. Samchil, Chuk, Seokgok extract refers to those of Examples 5, 11 and 17, respectively.

Composition Production example (weight) Comparative Example 4 10 11 12 -extract Ginseng extract 0.1 - - - Icicle extract - 0.1 - - Seokgok Extract - - 0.1 - Waselin 2.0 2.0 2.0 2.0 Sesquioleate sorbitan 0.8 0.8 0.8 0.8 Polyoxyethylene oleylethyl 1.2 1.2 1.2 1.2 Paraoxybenzoic acid methyl Quantity Quantity Quantity Quantity Propylene glycol 5.0 5.0 5.0 5.0 -ethanol 3.2 3.2 3.2 3.2 Carboxy vinyl polymer 18.0 18.0 18.0 18.0 Potassium Hydroxide 0.1 0.1 0.1 0.1 Pigment Quantity Quantity Quantity Quantity -incense Quantity Quantity Quantity Quantity -Purified water to 100 to 100 to 100 to 100

Preparation Example 13-15 and Comparative Example 5

Prescription examples of packs of cosmetics containing Samchil, Chuk, and Seokgok Extract are as follows. Samchil, Chuk, Seokgok extract refers to those of Examples 5, 11 and 17, respectively.

Composition Production example (weight) Comparative Example 5 13 14 15 -extract Ginseng extract 0.5 - - - Icicle extract - 0.5 - - Seokgok Extract - - 0.5 - -glycerin 5.0 5.0 5.0 5.0 Propylene glycol 4.0 4.0 4.0 4.0 Polyvinyl alcohol 15.0 15.0 15.0 15.0 -ethanol 8.0 8.0 8.0 8.0 -Polyoxyethylene oleyl ethyl 1.0 1.0 1.0 1.0 Paraoxybenzoic acid methyl 0.2 0.2 0.2 0.2 -incense Quantity Quantity Quantity Quantity Pigment Quantity Quantity Quantity Quantity -Purified water to 100 to 100 to 100 to 100

Preparation Example 16-18 and Comparative Example 6

Prescription examples of essences in cosmetics containing Samchil, Chuk, Seokgok extract are as follows. Samchil, Chuk, Seokgok extract refers to those of Examples 5, 11 and 17, respectively.

Composition Production example (weight) Comparative Example 6 16 17 18 -extract Ginseng extract 5 - - - Icicle extract - 5 - - Seokgok Extract - - 5 - Propylene glycol 10.0 10.0 10.0 10.0 -glycerin 10.0 10.0 10.0 10.0 Sodium hyaluronate aqueous solution (1) 5.0 5.0 5.0 5.0 -ethanol 5.0 5.0 5.0 5.0 Polyoxyethylene Cured Castor Oil 1.0 1.0 1.0 1.0 Paraoxybenzoic acid methyl 0.1 0.1 0.1 0.1 -incense Quantity Quantity Quantity Quantity -Purified water to 100 to 100 to 100 to 100

Experimental Example 3. Measurement of pigmentation inhibitory effect

Twenty healthy men and women were selected to attach aluminum foil with two rows of 6 holes with a diameter of 7 mm in the lower part of both arms, and 60mJ / cm ² light was irradiated using ORIEL solar simulator 1000W at a distance of 10cm from the arm. . The irradiation site was washed well with 70 ethanol aqueous solution before irradiation. Samples prepared according to Preparation Example 1-18 and Comparative Example 1-6 and Samchil, Chuk, and Seokgok Extracts, which were prepared according to Preparation Example 1-18 and Comparative Example 1-6, from 3 days before irradiation to 3 weeks after irradiation, were prepared. It was applied to the same row in pairs (for the pack formulations of Production Examples 13, 14, 15 and Comparative Example 5, they were removed 15 minutes after application). For each, the pigmentation degree of the production example and the comparative example was visually determined, and the degree of suppression of pigmentation was evaluated by the three steps of the distinctive effect, effect, and no difference compared to the comparative example, and the results are shown in the following table. Same as 4.

Inhibitory Effect of Pigmentation on Ointments and Cosmetics Containing Samchil, Cheong and Seokgok Extracts Experimental substance Distinct Effects (persons) Effective (persons) No difference (persons) Preparation Example 1 3 12 5 Preparation Example 2 2 11 7 Preparation Example 3 4 10 6 Preparation Example 4 2 9 9 Preparation Example 5 2 10 8 Preparation Example 6 2 9 9 Preparation Example 7 One 9 10 Preparation Example 8 One 9 10 Preparation Example 9 2 9 9 Preparation Example 10 0 9 11 Preparation Example 11 One 10 9 Preparation Example 12 One 9 10 Preparation Example 13 One 9 10 Preparation Example 14 One 10 9 Preparation Example 15 One 11 8 Preparation Example 16 2 7 11 Preparation Example 17 2 8 10 Preparation Example 18 3 8 9

As can be seen from the results in Table 4, the whitening ointment and cosmetics containing Samchil, Chuk, Seokok extract prepared according to Preparation Examples 1-18 showed a whitening effect on at least 9 or more of 20 subjects. Comparative experiments of Preparation Examples 1, 2, 3 and Comparative Example 1, in which a large amount of Samchil, Chuk, Seokgok extract were applied, showed 65-valent pigmentation inhibitory effect, and did not show any side effects in the skin. It can be seen that Seokgok extract is a safe and effective cosmetics for improving freckle and freckles and skin whitening.

As described above and as can be seen through the Examples and Comparative Examples, the present invention exhibits a very strong melanin synthesis inhibitory effect, and manufactures an external skin ointment or cosmetic containing dried samchil, cherries, and grains extracts for human skin. When applied to the blemishes, freckles and skin whitening effect is excellent and do not cause any side effects can provide a safe cosmetic composition.

Claims (6)

A cosmetic composition exhibiting blemishes, freckles, and skin whitening effects, comprising samchil, cherries, stone extract and at least one selected from them. The cosmetic composition according to claim 1, which contains 0.0001 -10.0 weight by weight of Samchil, Chuk, Seokgok Extract and at least one selected from them. The extract of claim 1, wherein the extract of Samchil, Chukwa, Seokgok is extracted one or more times with water, a lower alcohol having 1-4 carbon atoms, ethyl acetate or acetone, and the extract is concentrated under reduced pressure and freeze-dried or spray-dried. Cosmetic composition characterized in that. The cosmetic composition according to claim 2, wherein the cosmetic composition comprises 0.0005 to 10.0 wt.% Of dry weight, at least one selected from ginseng extract, spinach extract, and grains. The cosmetic composition according to claim 3, wherein the extraction solvent is 80 methanol. 4. The cosmetic composition according to claim 3, wherein the extraction solvent is ethyl acetate.