KR100797366B1 - Cosmetic composition containing Liquidambar taiwaniana Hance extracts and Cynanchum atratum Bunge extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition containing, as an active ingredient, at least one selected from roroton extract and white rice extract excellent in melanin production inhibitory effect.

Cosmetic composition containing at least one selected from Roroton, white rice extract of the present invention as an active ingredient shows a whitening effect and pigmentation inhibitory effect and does not show any side effects in the skin and is safe and excellent in improving freckles and freckles. .

Roroton, white rice, whitening effect, blemish, freckles

Description

Cosmetic composition containing at least one selected from Roroton extract, white rice extract as an active ingredient {Cosmetic composition containing Liquidambar taiwaniana Hance extracts and Cynanchum atratum Bunge extracts}

The present invention relates to a cosmetic composition containing at least one selected from roroton extract, white rice extract as an active ingredient, and more particularly, the present invention is selected from roroton extract, white rice extract excellent in melanin production inhibitory effect. It relates to a cosmetic composition containing at least a species as an active ingredient.

It is everyone's constant desire to have white, fair skin. Human skin color is genetically determined by the concentration and distribution of melanin in the skin, but is also influenced by environmental or physiological conditions such as solar ultraviolet rays, fatigue and stress. Melanin is a type of amino acid tyrosine (tyrosine) enzymes called tyrosinase (tyrosinase) acts to convert to dopa (DOPA), dopaquinone (dopaquinone) is produced through a non-enzymatic oxidation reaction. However, although the pathway by which melanin is made is known, it is still unknown in detail the mechanism by which tyrosinase acts, the mechanism that induces melanin synthesis. On the other hand, by inhibiting the synthesis of melanin pigments can not only realize skin whitening by brightening the skin tone, but also improve the hyperpigmentation of skin such as ultraviolet rays, hormones, blemishes caused by heredity, ultraviolet rays, freckles due to heredity. Therefore, conventionally, substances having inhibitory activity against tyrosinase, such as hydroquinone, ascorbic acid, kojic acid, and glutathione, are incorporated into ointments or cosmetics, and thus whitening, blemishes, and freckles of skin. Although it has been used for the purpose of improvement, the whitening effect of hydroquinone is recognized, but the amount of use is extremely limited due to severe skin irritation. In addition, ascorbic acid is easily oxidized, and the cosmetics containing the same cause problems such as discoloration and deodorization, and thiol-based compounds such as glutathione and cysteine have a characteristic unpleasant odor and have problems with transdermal absorption. In addition, these glycosides and derivatives have a high polarity, so there is a problem to use them as a component of a cosmetic. In addition, placenta extract has a problem that the whitening effect is insufficient.

Roroton is the fruit of the wind berry, Liquidambar taiwaniana Hance, which is a medicinal herb. Roroton has the effect of swelling and dehumidifying and has been used to treat hand and foot cramps, stomach pain, edema, amenorrhea, swelling, scabies and eczema (Chinese medicine dictionary). (JP08268837) It is irrelevant to the present invention.

White rice is a perennial herb belonging to the family Ju-Ki, with Latin name Radix Cynanchi Atrati and scientific name Cynanchum atratum Bunge. The taste is salty, the nature is limited and nontoxic. In addition, white rice has the effect of lowering heat, cooling blood, and treating antipyretic, diuretic, and small-scale customary pains (Chinese medicine dictionary, primary drug drug dictionary). , 14 (3), 138-42), and Glaucogenin A, C, H and Cynatratoside F (I) have been reported separately (Chem. Pharm. Bull. (1985), 33 (10), 4188-92)

Therefore, the present inventors have attempted to develop a new whitening agent for native animals and plants using B16 mouse melanoma cells that can screen for substances that inhibit melanin synthesis induction itself. As a result, Loroton extract, white rice extract was found to have a very strong melanin production inhibitory effect and whitening effect to complete the present invention.

In the present invention, the Rorotong, white rice, which can be purchased as a medicine, is first extracted with an organic solvent such as anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms or ethyl acetate or acetone, and then concentrated under reduced pressure, followed by freeze-drying or spray-drying. Was prepared. Roroton, white rice extract prepared by this method has a very potent melanin synthesis inhibitory effect in B-16 mouse melanoma cells. In addition, when the dermal ointment or cosmetics with dried Roroton, white rice extract is applied to human skin, the skin whitening effect is very excellent.

The present invention will be described in detail as follows.

Roroton, white rice, commercially available, is crushed and ground in an extractor equipped with a reflux condenser with 5-20 volumes of water or 1-4 anhydrous or hydrous lower alcohols, ethyl acetate or acetone, by weight of the dry matter. Extract by heating to 50-100 ^o C for 1-5 hours. After filtering with filter cloth, the residue is extracted one more time in the same manner. The extracts were combined and concentrated under reduced pressure, followed by freeze drying or spray drying to obtain a dry extract.

When the dried Roroton and white rice extracts were treated with rat melanoma cells (B-16 melanoma cells), they showed very potent melanin synthesis inhibitory effects.

Various cosmetics, such as a skin external ointment or lotion containing Roroton and white rice extract obtained as described above are prepared. Skin cosmetics such as roroton and white rice extracts, such as external application ointments and lotions were prepared and applied to human skin, and the skin whitening effect was very excellent. In addition, it does not cause any side effects, so Roroton, white rice extract is a safe and very effective blemishes and freckles improvement and skin whitening agent.

The content of Roroton and white rice extracts included in the preparation of external skin ointments or cosmetics is 0.0001-10.0% by weight, preferably 0.0005-10.0% by weight in dry weight. A clear effect could not be expected at concentrations below 0.0001% by weight, and no increase in effect was seen at concentrations above 10% by weight.

Next, the present invention will be described in detail with reference to Examples, and the present invention is not limited only to Examples.

Example 1-12

Purchasing commercially available Roroton and white rice, 100 g of pulverized powder is placed in 3 L of solvent such as water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate or acetone, and boiled for 3 hours in a reflux extractor with a cooling capacitor. After extraction, the resultant was filtered with 100 mesh filter cloth and the residue was extracted once more in the same manner. Each extract was combined and filtered using Whatman No. 2 filter paper at room temperature to remove insoluble materials, concentrated under reduced pressure at 60 ^o C in a distillation apparatus equipped with a cooling condenser, and lyophilized to dry weight as shown in Table 1 below. Got.

Dry weight of extractant and extract Roroton White rice 80% Methanol 7.5 g (Example 1) 9.8 g (Example 7) Purified water 6.8 g (Example 2) 8.2g (Example 8) 85% Ethanol 8.0g (Example 3) 9.3 g (Example 9) 70% Acetone 6.9 g (Example 4) 7.3 g (Example 10) Ethyl Acetate 6.3 g (Example 5) 7.2 g (Example 11) Saturated normal butanol 7.7g (Example 6) 8.6 g (Example 12)

Experimental Example 1

Each of the extracts obtained according to Examples 1-12 was added to the culture medium of B-16 mouse melanoma cells to test the whitening effect at the cellular level. (Lotan R., Lotan D. Cancer Res 40: 3345-3350, 1980). Each extract obtained according to Example 1-12 was added to a culture medium of B-16 melanoma cells with an aqueous hydroquinone solution and then cultured for 3 days with a final concentration of 0.005%, followed by trypsin treatment. Melanin was extracted from the culture vessel by centrifugation. To this was added sodium hydroxide solution (1N concentration) 1ml hydroxide rusted melanin boiled 10 minutes using a spectrophotometer at 400 nanometers (nm) The amount of the unit cell allowance (10 ⁶ cell) of melanogenesis by measuring the absorbance at It was carried out by the method represented by the absorbance, the relative melanin production relative to the control was calculated as the inhibition rate (%) and the results are summarized in Table 2.

As can be seen from the results in Table 2, considering that each sample obtained according to Examples 1-12 is an extract that is not purified at all, compared to the hydroquinone which is a known whitening substance, it is very good for the melanoma cells of cultured mice. It can be seen that there is a strong melanin production inhibitory ability.

Inhibitory Effects of Roroton and White Rice Extracts on the Cell Level of Melanin Production sample Melanin production % Inhibition Control group (no addition) 0.048 ± 0.003 - Example 1 (final concentration 0.005%) 0.030 ± 0.003 38% Example 2 (final concentration 0.005%) 0.033 ± 0.003 31% Example 3 (final concentration 0.005%) 0.028 ± 0.002 42% Example 4 (final concentration 0.005%) 0.026 ± 0.002 46% Example 5 (final concentration 0.005%) 0.023 ± 0.003 53% Example 6 (final concentration 0.005%) 0.030 ± 0.003 38% Example 7 (final concentration 0.005%) 0.034 ± 0.004 29% Example 8 (final concentration 0.005%) 0.036 ± 0.002 25% Example 9 (final concentration 0.005%) 0.033 ± 0.002 31% Example 10 (final concentration 0.005%) 0.027 ± 0.002 44% Example 11 (final concentration 0.005%) 0.025 ± 0.002 48% Example 12 (final concentration 0.005%) 0.022 ± 0.002 54%      Hydroquinone (final concentration 0.0001%) 0.025 ± 0.002 48%

                                                           * Number of iterations = 3

Experimental Example 2

Melamine production of rats cultured in the same manner as in Experimental Example 1 was investigated for the melanin production inhibitory ability according to the concentration of the extract of Examples 1, 7 and the results are shown in Table 3.

Inhibitory effect of melanin production at the cellular level sample Application concentration (%) Melanin production % Inhibition Control group (no addition) - 0.048 ± 0.004 - Example 1 Final concentration (0.0005%) 0.040 ± 0.002 17% Final concentration (0.005%) 0.030 ± 0.003 38% Final concentration (0.05%) 0.026 ± 0.002 46% Final concentration (0.5%) 0.022 ± 0.001 54% Example 7 Final concentration (0.0005%) 0.041 ± 0.002 7% Final concentration (0.005%) 0.034 ± 0.004 29% Final concentration (0.05%) 0.028 ± 0.002 42% Final concentration (0.5%) 0.022 ± 0.001 54% Hydroquinone (0.0001%) 0.025 ± 0.002 48%

Repeat number = 3

As can be seen in the results of Table 3, Roroton, white rice extract can be seen that it has a very strong melanin production inhibitory ability against cultured melanoma cells. Hydroquinone has a strong melanin production inhibitory ability at low concentrations, but the cytotoxicity is severe and can not be experimented at 0.0001% or more, whereas the extract of the present invention is the maximum amount of hydro that can be used without showing cytotoxicity at 0.05% or more concentration It has a higher melanin production inhibitory effect than quinone. In addition, Roroton, white rice extract has a very high melanin production inhibitory ability, considering that it is not a single substance but a mixture, it can be very useful for improving skin or freckles or skin whitening.

Hereinafter, the formulation example of the cosmetics containing Roroton and white rice extract was shown by the manufacture example.                     

Preparation Example 1-2 and Comparative Example 1

An example of external skin ointment containing Roroton and white rice extract is as follows.

Here, Roroton and white rice extracts refer to those of Examples 5 and 11, respectively.

Composition Production example (% by weight) Comparative example One 2 One -Extract Roroton Extract White Rice Extract                                               -Diethyl Sebacate-Brazing-Polyoxyethylene Oleyl Ether Phosphate-Sodium Benzoate-Vaseline  10-8 5 6 Suitable to 100  -10 8 5 6 Suitable to 100  --8 5 6 Suitable to 100

Preparation Example 3-4 and Comparative Example 2

A prescription example of a cream in cosmetics containing Roroton and white rice extract is as follows. Roroton and white rice extracts here refer to those of Examples 5 and 11.

Composition Production example (% by weight) Comparative example 3 4 2 -Extract Roroton Extract White Rice Extract                                              -Stearic acid-Cetanol-Potassium hydroxide-Glycerine-Propylene glycol-Preservative-Fragrance-Purified water  1.0-15.0 1.0 0.7 5.0 3.0 Proper quantity to 100   -1.0 15.0 1.0 0.7 5.0 3.0 Proper amount to 100  --15.0 1.0 0.7 5.0 3.0 Proper quantity to 100

Preparation Example 5-6 and Comparative Example 3

Roroton, a prescription example of the softening of the cosmetics containing white rice extract is as follows. Roroton and white rice extracts here refer to those of Examples 5 and 11.

Composition Production example (% by weight) Comparative example 5 6 2 -Extract Roroton Extract White Rice Extract                                              -Ethanol-Polylauric acid Polyoxyethylene sorbent Non-Paraoxybenzoic acid methyl-Glycerin-1,3-butylene glycol-Flavor-Pigment-Purified water  0.2-10.0 1.0 0.2 5.0 6.0 Proper volume to 100  -0.2 10.0 1.0 0.2 5.0 6.0 Proper amount to 100  --10.0 1.0 0.2 5.0 6.0 Proper amount to 100

Preparation Example 7-8 and Comparative Example 4

Roroton, a white rice extract containing a cosmetic example of nutritional longevity is as follows. Roroton and white rice extracts here refer to those of Examples 5 and 11.

Composition Production example (% by weight) Comparative example 7 8 4 -Extract Roroton Extract White Rice Extract                                              -Vaseline-Sesquioleate sorbitan-Polyoxyethylene oleyl ethyl-Paraoxybenzoic acid methyl-Propylene glycol-Ethanol-Carboxy vinyl polymer-Potassium oxalate-Pigment-Flavor-Purified water  0.1-2.0 0.8 1.2 appropriate 5.0 3.2 18.0 0.1 appropriate volume to 100  -0.1 2.0 0.8 1.2 Accurate 5.0 3.2 18.0 0.1 Accurate to 100  --2.0 0.8 1.2 Suitable 5.0 3.2 18.0 0.1 Suitable to 100

Preparation Example 9-10 and Comparative Example 5

Prescription of the pack of cosmetics containing Roroton, white rice extract is as follows. Here Rorotong, white rice extract refers to those of Examples 5 and 11, respectively.

Composition Production example (% by weight) Comparative example 9 10 5 -Extract Roroton Extract White Rice Extract                                              -Glycerine-Propylene glycol-Polyvinyl alcohol-Ethanol-Polyoxyethylene oleyl ethyl-Paraoxybenzoic acid methyl-Flavor-Pigment-Purified water  0.5-5.0 4.0 15.0 8.0 1.0 0.2 Proper volume to 100  -0.5 5.0 4.0 15.0 8.0 1.0 0.2 Correct Volume to 100  --5.0 4.0 15.0 8.0 1.0 0.2 Proper titration to 100

Preparation Example 11-12 and Comparative Example 6

Roroton, a prescription example of the assets in the cosmetics containing white rice extract is as follows. Roroton and white rice extracts here refer to those of Examples 5 and 11.

Composition Production example (% by weight) Comparative example 11 12 6 -Extract Roroton Extract White Rice Extract                                              -Propylene glycol-Glycerine-Sodium hyaluronate aqueous solution (1%)-Ethanol-Polyoxyethylene hardened castor oil-Paraoxybenzoic acid methyl-Flavor-Purified water  5-10.0 10.0 5.0 5.0 1.0 0.1 dose to 100  -5 10.0 10.0 5.0 5.0 1.0 0.1 dose to 100  --10.0 10.0 5.0 5.0 1.0 0.1 dose to 100

Experimental Example 3

Select 20 healthy males and females, and attach the aluminum foil with 6 holes of 7mm diameter in two rows to the lower forearm of each arm and irradiate 60mJ / cm ² light using ORIEL solar simulator 1000W at a distance of 10cm from the arm. It was. The irradiation site was washed well with 70% ethanol aqueous solution before irradiation. A pair of Roroton, a sample containing white rice extract prepared in accordance with Preparation Examples 1-12 and Comparative Examples 1-6, and a base not containing each extract, twice a day from 3 days before irradiation to 3 weeks after irradiation. (In the case of the pack formulations of Preparation Examples 9, 10, and Comparative Example 5, the coating was removed 15 minutes after application.) For each, the pigmentation degree of Preparation Example and Comparative Example was visually determined. Preparation Example evaluated the degree of inhibiting the pigmentation compared to the comparative example in three steps of distinct effects, effects, no difference, the results are shown in Table 4.

Inhibition of Pigmentation of Ointments and Cosmetics Containing Roroton and White Rice Extracts  Experimental substance Distinct Effects (persons) Effective (persons) No difference (persons) Preparation Example 1 4 8 8 Preparation Example 2 4 9 7 Preparation Example 3 3 8 9 Preparation Example 4 3 7 10 Preparation Example 5 2 9 8 Preparation Example 6 2 8 10 Preparation Example 7 2 8 10 Preparation Example 8 2 9 9 Preparation Example 9 2 10 8 Preparation Example 10 3 8 9 Preparation Example 11 3 8 9 Preparation Example 12 3 9 8

As can be seen from the results of Table 4, the Roroton, the whitening ointment and the cosmetic containing the white rice extract prepared according to Preparation Example 1-12 showed a whitening effect on at least 10 or more of 20 subjects, in particular In the comparative experiments of Preparation Examples 1 and 2 and Comparative Example 1 to which a large amount of Roroton and White rice extract were applied, 60% showed pigmentation inhibitory effect, and no side effects appeared in the skin. It can be seen that it is very effective in improving the appearance of freckles or skin whitening.

As described above and can be seen through the Examples and Comparative Examples, the cosmetic composition containing at least one selected from Roroton, white rice extract of the present invention as an active ingredient shows a whitening effect and pigmentation inhibitory effect in the skin Does not show any side effects and is safe and improves freckles and freckles.

Claims (3)

A cosmetic composition exhibiting blemishes, freckles, and skin whitening effects, comprising at least one selected from Roroton extract and Cynanchum atratum Bunge. Extract as an active ingredient. The cosmetic composition according to claim 1, which contains 0.0001 to 10.0% by weight of at least one selected from Roroton extract and Cynanchum atratum Bunge. Extract, respectively, in dry weight. According to claim 1, Rorotong extract and Cynanchum atratum Bunge. Extract is extracted one or more times with water, lower alcohol having 1 to 4 carbon atoms, ethyl acetate or acetone, respectively, the extract is distilled under reduced pressure and freeze-dried Cosmetic composition, characterized in that the extract obtained by spray drying.