KR100796014B1 - Hemostatic Extraction Method Using Samchil - Google Patents

Abstract

The present invention is effective to effectively stop various bleedings such as acute bleeding after childbirth, bruises caused by traffic accidents and bleeding after surgery such as surgery, plastic surgery, otolaryngology, etc. An excellent novel method for extracting from Sansuke and Samchil-Keun, hemostatic extracts are effectively collected and converted into distilled water, methanol, ethanol, or a mixed solvent thereof to form a cooling condenser, centrifugation method, and fockle. Granules, tablets or capsules can be taken anywhere by various methods such as rate, co-extrusion method, and effective extract ingredients that can be conveniently used in hemostatic areas are applied directly to the bleeding area by extracting, powdering and banding them. Or extracts the effective substances against hemorrhage of Samchilization and Samchil muscle, which can be used by bonding. The invention characterized by the method.

Description

Hemostatic Extraction Method Using Samchil {omitted}

The present invention relates to a method for effectively extracting a component effective for hemostasis from Sanchil, ie, Sanchilhwa or Samchil-geun.
In general, various methods and chemical agents are used for hemostasis. For example, hemostatic lead is used to mechanically stop bleeding. Orthopedic and otorhinolaryngology may stop the bleeding by applying it to the exposed bone. In addition, chemical substances such as thrombin, vitamin C, vitamin K, and calcium chloride may be used to coagulate blood. In small wounds, iodine tincture may be used to disinfect and hemostatically. In addition, folk remedies, such as falling from the mountain and wounds caused by the flow of blood, crush the clean blades of grass and apply the juice, and in oriental medicine, it is also used to take Huang (賣 連), Sanchija, loess (黄土).
On the other hand, Samchil to be dealt with in the present invention includes ethine phosphate, ethyl acetate, frfral alcohol, heptanol, octanal, benzeneacetaldehyde, camphor, brain, α copaethylene, α ethylene, β beta It contains high amounts of human active substances such as ethylene and α-monoalcohol, which can help higher secondary metabolic pathway (Sangseohan's Wang, 1989, 5 (2). P 42 and Lee Hyanggo's 'Herbal Private Medicine', 1986, 1 P 10).
In addition, Heo Joon's consent is written, "Shamil is used to stop the blood flowing", as well as the non-toxicity of three-three, that is, harmless to the human body and safe.

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It is an object of the present invention to provide a method for effectively extracting substances having a great effect on hemostasis from ginseng, which contains a large amount of ingredients effective in the human body, which is harmless to the human body. That is, the purpose of the present invention is to propose a new method of extracting hemostatic agents from samchil.
In addition, the present invention provides an effective method for extracting a substance excellent in hemostasis from ginseng, by showing an example that can be beneficially applied to the human mechanism of ginseng, biotechnology and biotechnology (21) BIO) Another purpose is to contribute to the development of the industry.
As described above, the present invention aims to provide a novel method for effectively extracting the components excellent in hemostasis from samchil, the extract of Samchil extracted according to the method of the present invention is liquid or concentrated as well as the stock solution itself. It may be used by any method such as spray-dried granules, capsules or tablets. That is, it can be used in various forms for the purpose of hemostasis in any form, such as liquid, powder, granules, tablets, and also within the scope of not impairing the object of the present invention, for example, sodium benzoate or sulfuric acid which is a conventional additive ( Coptis chinensis), hawthorn, Aya (艾葉), loess (黄土), licorice (Glycyrrhiza uralensis), royal jelly, vitamins C and K can also be used.

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In the following, distilled water, methanol, ethanol, or mixed solvents may be extracted from each of the three-sylated and three-muscle roots using various methods such as a cooling condenser, centrifugal separation, folate, and reduced pressure extrusion. Bar, among the various methods, some embodiments that can increase the extraction efficiency of the active ingredient.

Example 1 Extraction Method from Saccharification

First, after pickling and cutting, select 20 ~ 30% by weight ratio and put it in a suitable container. Acid concentration such as lemon juice, citric acid or amino acid or pH 4.5 ~ 6 in order to suppress microbial contamination and effectively extract the unique active ingredient 70% to 80% of the water adjusted in the mixture was mixed again and then wetted in a closed state at room temperature for 24 to 48 hours. Appears in combination with water mixed in.

This was again disinfected by heating at 70-80 ° C. for 40-45 minutes, sterilized by distilling it on a filter cloth, and then decomposing pectin or hemicellulose, the main component of the turbidity, as a component of the cell membrane in the three-saturated extract undiluted solution. 0.01 ~ 0.03% of soluble enzyme Pectinase (to% by weight of the three-saturated stock solution) was added, stirred again, and left for 2-3 days to precipitate out and then precipitated as a clear solution. 30% by weight of the residue remaining on the leachate and the filter cloth was put in a suitable container (glass, plastic, glass container) and stirred by reacting 40% ethanol (Ethannol) with 30% thickened water as a solvent. The second leachate is collected by using hot water heated at 60 to 70 ° C. for 1 to 2 hours using a known device such as a press machine or a centrifuge.

At this time, the first leachate is 80%, and the second leachate is heated to 100% by adding 20% by concentrating while evaporating ethanol. Another method is to trim the 20% of the three-methylation in weight ratio, and 30% of acid, that is, adjusted to pH 4.5 to 4.6, is wetted for 24 to 48 hours at room temperature and then wetted with 40% of ethanol as in the former method. Mix 10% reacted.

This is put into the extractor equipped with a cooling condenser again and heated to 70 to 80 degrees for 8 to 30 hours to obtain a clear extract peculiar to samchilization.

Heat is added to the extract thus obtained to evaporate the ethanol to give a mysterious extract containing trembling, ginseng-flavor-like taste and an active ingredient that can help the physiological activity of vivid tricolor of pale yellow.

The extract thus obtained is finally sterilized and then obtained in the form of a concentrate while concentrated under reduced pressure at 70 to 80 ° C., or the viscous concentrate (about 60% of water) is sprayed at 120 ° C. to 160 ° C. Spray drying with a dryer forms granules. Samchilhwa's unique extract is concentrated in such a pharmacological component is a substance that can be very effectively used as a hemostatic agent by taking the human body or apply to the affected area.

Example 2. Extraction Method from Machimus

The difference is that the three roots are dried in a shade in a blower or in oils such as hot cooking oil and then dried at a weak heat of 30-40 ° C. It is the same as the extraction method from samilization which was demonstrated in Example 1. That is, from the three muscles, distilled water, methanol, ethanol or a mixed solvent in the form of a cooling condenser, centrifugal separation, folate, reduced pressure extrusion method using the same method as described in Example 1 to extract the three thousand extract do.

- : 효과가 약함 - 7 분내 지혈
+ : 효과가 조금 나타난 경우 - 5 분내 지혈
++ : 효과가 많이 나타난 경우 - 3 분내 지혈
+++ : 효과가 아주 많이 나타난 경우 - 1 분내 지혈
A 군 : 일반적으로 사용되는 염화칼슘제 적용
B 군 : 혈관수축제 아드레날린 적용
C 군 : 요오드 팅크 적용
D 군 : 알미늄염 적용
E 군 : 본 발명의 방법에 따라 추출된 삼칠 추출물 적용
F 군 : 본 발명의 방법에 따라 추출된 삼칠 추출물 적용
G 군 : 본 발명의 방법에 따라 추출된 삼칠 추출물 적용
상기 표 1로부터, 본 발명의 방법에 따라 추출된 삼칠 추출물이 기존의 지혈 방법과 비교할 때, 지혈에 탁월한 효과가 있음을 분명하게 확인할 수 있다.
2. 임상 실험
동물 실험에 이어, 앞서 설명한 실시예 1 및 실시예 2의 방법을 사용하여 추출한 삼칠 추출물을 사용하여 임상 실험을 행하였다. 즉, A 내지 G의 손 등에 찰과상을 입어 출혈이 진행 중인 7명의 환자에 대해, A, B, C, D군은 기존의 화학 약제 등을 사용하여 비교군으로 하고 E, F, G군은 본 발명의 방법에 따라 추출된 삼칠 추출물을 사용하여 지혈함으로써, 지혈 효과를 비교하는 실험을 하였다. 삼칠 추출물은 그 진액을 정제 또는 캡슐화시켜서 복용시키거나 또는 출혈 환부에 직접 도포하는 방식으로 사용하였다. 표 2는 임상 실험 결과를 나타내는 표이다.

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- : 효과가 약함 - 5 분내 지혈
+ : 효과가 조금 나타난 경우 - 3 분내 지혈
++ : 효과가 많이 나타난 경우 - 2 분내 지혈
+++ : 효과가 아주 많이 나타난 경우 - 30초 내 지혈
A 군 : 일반적으로 사용되는 염화칼슘제 적용
B 군 : 혈관수축제 아드레날린 적용
C 군 : 요오드 팅크 적용
D 군 : 알미늄염 적용
E 군 : 본 발명의 방법에 따라 추출된 삼칠 추출물 적용
F 군 : 본 발명의 방법에 따라 추출된 삼칠 추출물 적용
G 군 : 본 발명의 방법에 따라 추출된 삼칠 추출물 적용
상기 표 2로부터, 본 발명의 방법에 따라 추출된 삼칠 추출물이 기존의 지혈 방법과 비교할 때, 동물 실험에서뿐만 아니라 임상 실험에 있어서도 지혈에 탁월한 효과가 있음을 분명하게 확인할 수 있다.1. Animal Experiment
Animal experiments were performed as indicated in Table 1 below, using the Samchil extract extracted using the methods of Example 1 and Example 2 described above. That is, as shown in Table 1, after wounding 2 mm in diameter and 1 cm in depth on the back of the seven (1 year old) rats of A to G, A, B, C, D group as a comparison group And E, F, G group was experimented to compare the hemostatic effect by hemostasis using the extract of the present invention.

-: Weak effect-hemostasis within 7 minutes
+: Slight effect-hemostasis within 5 minutes
++: If the effect appeared a lot-hemostasis in 3 minutes
+++: If the effect appeared very much-hemostasis in 1 minute
Group A: Application of commonly used calcium chloride
Group B: Applying Vasoconstrictor Adrenaline
Group C: Iodine tincture applied
Group D: Aluminum Salt Application
E group: Application of the three-month extract extracted according to the method of the present invention
Group F: Apply the three-month extract extracted according to the method of the present invention
Group G: Samchil extract applied according to the method of the present invention
From Table 1, it can be clearly seen that Samchil extract extracted according to the method of the present invention has an excellent effect on hemostasis when compared with the conventional hemostatic method.
2. Clinical trial
Following animal experiments, clinical trials were conducted using three-million extracts extracted using the methods of Example 1 and Example 2 described above. That is, for seven patients whose bleeding is progressing due to abrasions on the hands of A to G, groups A, B, C, and D are used as a comparison group using conventional chemical agents, and E, F, and G groups Hemostasis was performed using the Samchil extract extracted according to the method of the invention, and experiments were performed to compare the hemostatic effects. Shamil extract was used in the form of tablets or encapsulation of the essence or applied directly to the bleeding affected area. Table 2 shows the results of clinical trials.

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-: Weak effect-hemostasis within 5 minutes
+: If the effect appeared a little-hemostasis in 3 minutes
++: If the effect appeared a lot-hemostasis in 2 minutes
+++: If the effect appeared very much-hemostasis within 30 seconds
Group A: Application of commonly used calcium chloride
Group B: Applying Vasoconstrictor Adrenaline
Group C: Iodine tincture applied
Group D: Aluminum Salt Application
E group: Application of the three-month extract extracted according to the method of the present invention
Group F: Apply the three-month extract extracted according to the method of the present invention
Group G: Samchil extract applied according to the method of the present invention
From Table 2, it can be clearly seen that Samchil extract extracted according to the method of the present invention has an excellent effect on hemostasis not only in animal experiments but also in clinical trials when compared to conventional hemostatic methods.

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Claims (1)

As a method of extracting a substance effective for hemostasis from trichophytopea or trichophyte, Fermentation aging of the three-sylated or three-skilled muscle or fermented with acid or ethanol (Ethanol) or water adjusted to a pH 4.5 ~ 6.5 concentration as a solvent, after heating and dissolving, reduced pressure at 70 ~ 80 ℃ Concentrate to extract the extract (about 60% moisture) or spray-dried at 120 ~ 160 ℃ to extract a powder or granular phase.