KR101451981B1 - Compositions comprising fermentative extract of Orchidaceae for improving functions of skin - Google Patents
Compositions comprising fermentative extract of Orchidaceae for improving functions of skin Download PDFInfo
- Publication number
- KR101451981B1 KR101451981B1 KR1020080009033A KR20080009033A KR101451981B1 KR 101451981 B1 KR101451981 B1 KR 101451981B1 KR 1020080009033 A KR1020080009033 A KR 1020080009033A KR 20080009033 A KR20080009033 A KR 20080009033A KR 101451981 B1 KR101451981 B1 KR 101451981B1
- Authority
- KR
- South Korea
- Prior art keywords
- orchid
- skin
- mushroom
- extract
- fermented extract
- Prior art date
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
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- A—HUMAN NECESSITIES
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
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- General Health & Medical Sciences (AREA)
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- Cosmetics (AREA)
Abstract
본 발명은 난초를 버섯균으로 발효시킨 후 추출하는 단계를 포함하는 피부 보호 및 개선, 피부 미백, 피부 노화 및 주름의 예방 또는 개선, 피부 보호 및 피부의 염증 반응의 완화, 및/또는 피부 장벽 기능 개선을 위한 난초의 버섯균 발효 추출물의 제조 방법, 그에 의하여 제조된 난초의 버섯균 발효 추출물, 및 그를 포함하는 화장료 조성물 및 기능성 식품에 관한 것이다.The present invention relates to a method for preventing or improving skin protecting and improving, skin whitening, prevention or improvement of skin aging and wrinkles, fermenting an orchid as mushroom bacteria and extracting it, skin protection and alleviation of skin inflammation reaction, and / The present invention relates to a method for producing a fermented extract of mushroom mycorrhiza for orchid improvement, a fermented extract of mushroom mycorrhizae produced thereby and a cosmetic composition and a functional food containing the same.
화장료, 발효 추출물, 미백, 멜라닌, 노화, 주름개선, 콜라겐, 염증완화, 피부장벽, 난초, 풍란, 죽백란, 제주 한란, 석곡(장생란) Collagen, inflammation relief, skin barrier, orchid, windsurfing, bamboo blanket, Jeollanam, ganggok,
Description
본 발명은 난초를 버섯균으로 발효시킨 후 추출하는 단계를 포함하는 피부 보호 및 개선, 피부 미백, 피부 노화 및 주름의 예방 또는 개선, 피부 보호 및 피부의 염증 반응의 완화, 및/또는 피부 장벽 기능 개선을 위한 난초의 버섯균 발효 추출물의 제조 방법, 그 방법에 의하여 제조된 난초의 버섯균 발효 추출물, 및 그를 포함하는 화장료 조성물 및 기능성 식품에 관한 것이다.The present invention relates to a method for preventing or improving skin protecting and improving, skin whitening, prevention or improvement of skin aging and wrinkles, fermenting an orchid as mushroom bacteria and extracting it, skin protection and alleviation of skin inflammation reaction, and / The present invention relates to a method for producing a fermented extract of mushroom mycorrhiza of orchids, an extract of fermented mushroom mycorrhizae prepared by the method, and cosmetic compositions and functional foods containing the same.
난초는 외떡잎식물 중에서 가장 진화된 식물군으로, 아름답고 향기로운 꽃이 피는 종으로 세계에 약 700속 2만 5000종이 알려져 있고, 한국 자생종은 39속 84종이다. 난초는 양극지방을 제외한 전 세계에서 자라며, 특히 열대지방의 운무림(雲霧林)에서 많이 자라는 여러해살이 식물이다. 예로부터 난초는 식용뿐만 아니라 약용으로 이용되어 왔을 만큼 다양한 유효 성분을 함유하고 있다. 특히, 수분 보 유능이 우수하고 미백 효과가 있어 화장품 원료로도 사용되고 있다. 예를 들어, 국제공개 특허 WO 2004/093839는 백란, 특히 팔레놉시스 아마빌리스 난초의 지상부로부터의 추출물을 포함하는 피부미백 특성을 갖는 화장품을 개시하고 있다.The orchid is the most evolved plant group among the monocotyledonous plants. It is a beautiful and fragrant flower bloom. There are about 25,000 species in the world and about 25,000 species in the world. The Korean native species is 39 genera and 84 species. An orchid grows all over the world except for anodic fat, and is a perennial plant that grows especially in the cloud forest of tropical regions. From the past, orchids contain as many active ingredients as they have been used for edible as well as medicinal purposes. In particular, it has excellent moisture-retaining ability and has a whitening effect and is used as a cosmetic raw material. For example, WO 2004/093839 discloses cosmetics having skin whitening properties comprising extracts from white rye, especially from above ground of Palenopsis amarbis orchids.
그러나 난초 추출물을 피부 적용 시 피부 자극 등의 안전성 문제로 사용량의 제한이 있거나, 효과가 미미하여 실질적으로 피부 기능 개선 효과를 기대할 수 없었다. 따라서, 기존의 피부 외용제 조성물보다 생체에 안전하고 효과가 높은 새로운 피부 외용제 조성물의 개발이 절실히 요망되고 있다.However, when applying the orchid extract to the skin, there is a limitation in the use amount due to a safety problem such as skin irritation or the effect is insignificant, so that the effect of improving the skin function substantially can not be expected. Therefore, it is urgently required to develop a novel composition for external skin application which is safer and more effective in living body than existing skin external composition compositions.
한편, 미생물이 가지고 있는 효소를 이용하여 유기물을 분해시키는 과정을 발효라고 한다. 발효와 부패는 비슷한 과정에 의해 진행되지만 분해 결과 우리의 생활에 유용하게 사용되는 물질이 만들어지면 발효라 하고, 악취가 나거나 유해한 물질이 만들어지면 부패라고 한다. On the other hand, fermentation is a process of decomposing organic matter using enzymes possessed by microorganisms. Fermentation and corruption proceed by a similar process, but decomposition is called fermentation when substances that are useful for our lives are made, and when they make odorous or harmful substances, it is called corruption.
이에 본 발명자들은 상기한 문제점들을 해결하고자 노력하였는바, 난초 추출물을 발효시키는 경우 난초의 독성이 감소되고, 저분자화되어 소화가 용이하며, 피부에 도포시 경피흡수 등이 용이하게 되어 생체 이용률이 향상됨을 발견하였다. 또한 난초추출물은 그 자체로도 효능 효과를 나타낼 수 있지만 발효를 통해 효능 효과가 더욱 강화되고 미생물의 대사에 의해 이로운 성분들이 만들어져 발효전의 추출물에 없던 새로운 효능을 발휘할 수 있음을 발견하고, 본 발명을 완성하게 되었다.Accordingly, the present inventors have endeavored to solve the above problems. When the orchid extract is fermented, the toxicity of the orchid is reduced, the molecular weight is lowered, the digestion is easy, and the bioavailability is improved by facilitating transdermal absorption upon application to the skin . In addition, it has been found that the orchid extract itself can exhibit the efficacy by itself, but it has been found that fermentation enhances the efficacy of the fermentation and that components beneficial to the metabolism of the microorganism are produced, It was completed.
본 발명의 하나의 목적은 난초의 효능 및 효과를 더욱 강화시키고자 난초를 버섯균으로 발효시킨 후 추출하는 단계를 포함하는 피부 보호 및 개선, 피부 미백, 피부 노화 및 주름의 예방 또는 개선, 피부 보호 및 피부의 염증 반응의 완화, 또는 피부 장벽 기능 개선을 위한 난초의 버섯균 발효 추출물의 제조 방법 및 그 방법에 의하여 제조된 난초의 버섯균 발효 추출물을 제공하는 것이다.It is an object of the present invention to provide a method for protecting or improving skin, comprising the step of fermenting orchid as mushroom germ and further extracting the orchid to further enhance the efficacy and effectiveness of the orchid, the prevention or improvement of skin whitening, skin aging and wrinkles, And to provide a method for producing an extract of fermented mushroom mycorrhiza of an orchid for alleviating inflammation reaction of the skin or for improving skin barrier function and a fermented extract of mushroom mycelia produced by the method.
본 발명의 다른 하나의 목적은 상기 난초의 버섯균 발효 추출물을 포함하는, 피부에 안전하면서도 멜라닌합성을 저해하고 프로콜라겐 합성 촉진 효과가 우수하며 자외선에 대한 피부 손상 방지 및 염증반응을 억제하고 피부 장벽 기능 회복을 강화하는 효능을 갖는 화장료 조성물 및 기능성 식품을 제공하는 것이다. Another object of the present invention is to provide a method for inhibiting melanin synthesis and promoting pro-collagen synthesis, which is safe to the skin, including fermented extract of mushroom mycobacteria of the above-mentioned orchid, inhibits skin damage to ultraviolet rays and suppresses inflammation reaction, And to provide a cosmetic composition and a functional food having an effect of enhancing functional recovery.
하나의 양태로서, 본 발명은 난초를 버섯균으로 발효시킨 후 추출하는 단계를 포함하는 피부 보호 및 개선, 피부 미백, 피부 노화 및 주름의 예방 또는 개선, 피부 보호 및 피부의 염증 반응의 완화, 및/또는 피부 장벽 기능 개선을 위한 난초의 버섯균 발효 추출물을 제조하는 방법에 관한 것이다.In one aspect, the present invention relates to a method for preventing or improving skin protection and improvement, skin whitening, preventing or improving skin aging and wrinkles, fermenting orchid as mushroom bacteria and extracting the skin, / RTI ID = 0.0 > mushroom < / RTI > fermentation extract of orchid for improving skin barrier function.
본 발명의 난초는 난과(Orchidaceae)에 속하는 식물로서, 구체적으로는 아칸토피피움 블룸속(Acanthophippium Blume), 아시안터스속(Acianthus), 아이리데스 속(Aerides), 앙그라이쿰속(Angraecum), 아미토스티그마속(Amitostigma), 아노엑토실러스속(Anoectochilus), 안셀리아속(Ansellia), 아플렉트럼속(Aplectrum), 아레테사속(Arethusa), 아룬디나 블룸속(Arundina Blume), 아스코켄트룸속(Ascocentrum), 브라시아속(Brassia), 벨티라속(Beltilla), 불보필럼속(Bulbophyllum), 칼란테속(Calanthe), 칼립소속(Calypso), 케틀레야속(Cattleya), 세파란테라속(Cephalanthera), 코엘로지네속(Coelogyne), 코에로그로숨속(Coeloglossum), 코렐로이자속(Corallorhiza), 크레마스트라속(Cremastra), 심비디움속(Cymbidium), 시프리페디움속(Cypripedium), 크리토포디움속(Crytopodium), 덴드로비움속(Dendrobium), 엘레오르키스속(Eleorchis), 에피덴드럼속(Epidendrum), 에피포기움속(Epipogium), 에리아속(Eria), 유로피아속(Eulophia), 갈레올라속(Galeola), 구우디에라속(Goodyera), 게오도룸속(Geodorum), 짐나데니아속(Gymnadenia), 하베나리아속(Habenaria), 한코키아속(Hancockia), 허미니움속(Herminium), 키티고르키스속(Kitigorchis), 라일리아속(Linaria), 리파리스속(Liparis), 리스테라속(Listera), 리카스테속(Lycaste), 마코드스속(Macodes), 말라시스 솔란드속(Malaxis Soland.), 마이크로스타일리스속(Microstylis), 네오피네티아속(Neofinetia), 네오띠아속(Neottia), 온시디움속(Oncidium), 오레오르키스속(Oreorchis), 팔라에높시스속(Phalaenopsis), 프라그모페딜룸속(Phagmopedium), 폴리도타속(Pholidota), 플라탄테라속(Platanthera), 포고니아속(Pogonia), 린코스타일리스속(Rhynchostylis), 사르칸투스속(Sarcanthus), 스피란터스속(Spiranthes), 스파쏘글로티스 블룸속(Spathoglottis Blume), 티푸라리아속(Tipularia), 타이니아속(Tainia), 반다속(Vanda), 벡실라비움속(Vexillabium), 지고페탈룸속(Zygopetalum) 등의 속(genus)에 속하는 난초과 식물을 말한다. 본 발명의 구체적 실시에서는 죽백란(Cymbidium lancifolium), 제주 한란(Cymbidium orchids), 석곡(장생란) (Dendrobium) 및 풍란(Neofinetia falcata)의 난초과 식물을 사용하였다. The orchid of the present invention belongs to the genus Orchidaceae and is specifically a plant belonging to the genus Acanthopipium Blume, Acianthus, Aerides, Angraecum, Amitostigma, Anoectochilus, Ansellia, Aplectrum, Arethusa, Arundina Blume, Asco-Kent, (Ascocentrum), Brassia, Beltilla, Bulbophyllum, Calanthe, Calypso, Cattleya, Cephalanthera, ), Coelogyne, Coeloglossum, Corallorhiza, Cremastra, Cymbidium, Cypripedium, Cryptobacillus, Such as Crytopodium, Dendrobium, Eleorchis, Epidendrum, Epipogium, Eria, Eulophia, Galeola, Goodyera, Geodorum, Gymnadenia, and so on. Habenaria, Hancockia, Herminium, Kitigorchis, Linaria, Liparis, Listera, Rica, and the like. Lycaste, Macodes, Malaxis Soland., Microstylis, Neofinetia, Neottia, Oncidae, and so on. Oncidium, Oreorchis, Phalaenopsis, Phagmopedium, Pholidota, Platanthera, Pogonia, Such as Rhynchostylis, Sarcanthus, Spiranthes, Spathoglottis Blume, T. spp. refers to an orchid plant belonging to the genus, such as the spider, ipularia, Tainia, Vanda, Vexillabium, Zygopetalum. In a specific embodiment of the present invention, Cymbidium lancifolium ), Cymbidium orchids , Dendrobium , and Neofinetia falcata ) orchid plants were used.
상기 죽백란(Cymbidium lancifolium)은 난초과에 속하는 여러해살이풀로서 돈란이라고도 한다. 산 중턱 숲속에서 자라며, 마디 부분과 잎이 대나무와 비슷하여 죽백이라는 이름이 붙었다. 죽백란은 햇볕에 강하고 꽃이 아름다워 관상적 가치가 크며, 주로 한국(제주도 한라산)에 분포한다. 상기 제주 한란(Cymbidium orchids)은 천연기념물 제191호로 상록 여러해살이풀로서, 난초 중에서 가장 귀중한 종으로 제주 남쪽 산허리 일대에서 자생한다. 상기 석곡(Dendrobium)은 난과의 2번째로 큰 속으로 다년생 초본식물로 바위나 죽은 나무 줄기에 붙어서 자란다. 한방에서는 뿌리를 제외한 식물체 전체를 약재로 쓰는데, 해열 및 진통 작용이 있으며, 백내장에 효과가 있고, 건위제 및 강장제로 사용한다. 석곡은 주로 한국(제주, 전남, 경북), 일본 및 중국 등지에 분포한다. 상기 풍란(Neofinetia falcata)은 조란(弔蘭)이라고도 하며, 나무줄기와 바위 겉에 붙어서 자라는 착생란으로 높이 3∼15cm의 짧은 줄기 몇 개가 모여서 함께 자라며, 잎은 좌우 2줄로 빽빽이 나고 넓은 줄 모양이다. 잎은 딱딱하고 2개로 접히며 윗부분은 뒤로 젖혀지고 밑에 환절이 있다. 순백색의 꽃이 7월에 피고, 한국(남쪽 섬) 및 일본에 주로 분포한다. The Cymbidium lancifolium ) is a perennial herb that belongs to the orchidaceae family. It grows in the mid-mountain forests, and its nodule and leaves are similar to bamboo, so it is named Bamboo. The bamboo leaves are strong in the sun and have beautiful flowers and are of great value. They are mainly distributed in Korea (Halla Mountain, Cheju Island). The Cymbidium orchids is a natural monument No. 191, an evergreen perennial herb that is the most precious species of orchids and is native to the southern hillsides of Jeju. Dendrobium is the second largest genus of the egg and is a perennial herbaceous plant that grows on a rock or dead tree trunk. In herbal medicine, the whole plant except root is used as a medicinal material. It has antipyretic and analgesic effect, and is effective for cataract, and is used as a raw material and a tonic. Gokgok is mainly distributed in Korea (Jeju, Chonnam, Gyeongbuk), Japan and China. The above- falcata ) is also called as 蘭 蘭 (蘭 蘭). It grows on tree stem and rock surface. It grows together with several short stems of 3 ~ 15cm in height. Leaves are hard and folded in two, with the upper part bent backward and the lower part bent. Pale white flowers bloom in July, and are distributed mainly in Korea (southern islands) and Japan.
본 발명의 상기 난초는 야생종 또는 인간에 의하여 재배(예를 들면 하우스 재배, 이식 재배)된 것의 어느 것이라도 사용할 수 있다. 또한, 본 발명의 상기 난초는 난초의 다양한 기관(예로, 꽃, 줄기 또는 잎, 뿌리 등)을 사용하거나 또는 난초 전체를 사용할 수 있다. The orchid of the present invention can be used either as a wild type or as a plant grown by humans (for example, house cultivation, transplant cultivation). In addition, the orchid of the present invention may use various organs of orchids (e.g., flowers, stems or leaves, roots, etc.) or whole orchids.
본 발명의 상기 버섯균은 식용으로 가능한 버섯균이면 어느 것이나 가능하나, 바람직하게는 차가버섯(Inonnotus - obliquus), 동충하초균류(Cordyceps sinensis , Paecilomyces japonica), 송이버섯(Tricholoma matsutake), 상황버섯(Phellinus linteus) 또는 영지버섯(Ganoderma lucidum)을 말한다.The fungus of the present invention the fungus is available for human consumption can be one which would, preferably, Chaga (Inonnotus - obliquus), the fungus Cordyceps sinensis (Cordyceps sinensis , Paecilomyces japonica ), pine mushroom ( Tricholoma matsutake ), mushroom ( Phellinus linteus or Ganoderma lucidum .
본 발명의 난초 버섯균 발효 추출물은 난초를 버섯균으로 발효하여 추출하는 단계, 구체적으로는 난초를 버섯균으로 발효하여 발효물을 수득하는 단계 및 상기 발효물을 추출 용매를 이용하여 추출하는 단계를 포함하는 방법에 의하여 제조할 수 있다. 상기 발효 및 추출 방법은 당해 분야에서 잘 알려진 방법에 의하여 이루어질 수 있다. 예를 들면, 난초를 통상의 방법에 따라 잘게 분쇄하고 멸균한 후, 이에 버섯균을 접종하여 적정한 온도 및 습도에서 적정한 시간 동안 발효시킨 다음, 이들 발효물로부터 당해 분야에 알려진 방법에 의하여 추출을 함으로써 제조할 수 있다. 상기 난초의 버섯균에 의한 적정 온도는 18 내지 25℃이며, 적정 습도는 60 내지 80%이며, 적정 시간은 1일 내지 100일이다. 상기 추출의 구체적 방법은 예를 들면, 물이나 탄소수 1 내지 4개의 무수 또는 함수 저급 알코올(예로, 메탄 올, 에탄올, 프로판올 및 부탄올 등), 상기 저급 알코올과 물의 혼합 용매, 아세톤, 에틸아세테이트, 클로로포름, 1,3-부틸렌글리콜, 헥산 및 디에틸에테르로 구성된 군으로부터 선택되는 어느 하나의 용매, 바람직하게는 물 또는 탄소수 1 내지 4개의 무수 또는 함수 저급 알코올로 환류냉각기가 달린 추출기에 50 내지 100℃, 1 내지 5시간 동안 가열하여 감압 농축하는 방법이다. 상기 추출 용매의 양은 이용되는 추출 용매의 종류에 따라 다양할 수 있으나, 전형적으로 발효물의 건조 중량에 대하여 1 내지 20배이며, 바람직하게는 5 내지 20배이다. 또한, 상기 추출은 1회 이상 실시될 수 있으며, 또한 1회 이상 추출한 추출액을 혼합하고, 이를 감압 농축할 수도 있다. The fermentation extract of orchid mushroom of the present invention comprises the steps of fermenting orchid and extracting orchid as mushroom bacteria, specifically extracting a fermented product by fermenting orchid as mushroom germ, and extracting the fermented product with an extraction solvent And the like. The fermentation and extraction methods may be performed by methods well known in the art. For example, the orchid is finely pulverized and sterilized according to a usual method, the mushroom is inoculated thereto, fermented at an appropriate temperature and humidity for a suitable time, and then extracted from the fermented product by a method known in the art Can be manufactured. The optimum temperature by the mushroom of the orchid is 18 to 25 캜, the optimum humidity is 60 to 80%, and the optimum time is 1 to 100 days. Specific examples of the extraction include water or anhydrous or lower alcohol having 1 to 4 carbon atoms (for example, methanol, ethanol, propanol and butanol), a mixed solvent of the lower alcohol and water, acetone, ethyl acetate, chloroform , 1,3-butylene glycol, hexane, and diethyl ether, preferably water or an anhydrous or hydro-lower alcohol having 1 to 4 carbon atoms in an extractor equipped with a reflux condenser, at 50 to 100 Deg.] C for 1 to 5 hours to concentrate under reduced pressure. The amount of the extraction solvent may vary depending on the kind of the extraction solvent used, but is typically 1 to 20 times, and preferably 5 to 20 times, the dry weight of the fermentation product. The extraction may be carried out at least once, and the extract obtained by one or more extractions may be mixed and concentrated under reduced pressure.
상기한 발효 및 추출 과정에서 난초를 버섯균으로 발효하기 이전에 난초에 접종된 버섯균이 잘 배양되도록 난초의 수분 함량을 40 내지 60 중량%로 조절할 수 있다. 또한, 버섯균을 접종하기 이전에 수분 함량이 적절하게 조절된 난초를 멸균하는 것이 바람직하다. 상기 멸균 방법은 당해 분야에서 통상적으로 실시되는 어떠한 멸균 방법을 이용할 수 있으며, 예를 들어, 고압 멸균 방법, 고온 멸균 방법 및 저온 멸균 방법을 통하여 실시할 수 있다. 구체적인 실시예로서, 121℃에서 15분간 습윤 멸균한다.The water content of the orchid can be adjusted to 40 to 60% by weight so that the mushroom bacteria inoculated in the orchid is cultured well before the fermentation of the orchid into the mushroom germ in the fermentation and extraction process. In addition, it is preferable to sterilize the orchid with an appropriate moisture content before inoculation of the mushroom. The sterilization method may be carried out by any sterilization method commonly practiced in the art, for example, a high pressure sterilization method, a high temperature sterilization method, and a low temperature sterilization method. As a specific example, it is wet sterilized at 121 DEG C for 15 minutes.
본 발명의 추출물은 상술한 추출 용매에 의한 추출물 뿐만 아니라, 통상적인 정제 과정을 거친 추출물도 포함한다. 예를 들어, 일정한 분자량 컷-오프 값을 갖 는 한외여과막을 이용한 분리, 다양한 크로마토그래피 (크기, 전하, 소수성 또는 친화성에 따른 분리를 위해 제작된 것)에 의한 분리 등, 추가적으로 실시된 다양한 정제 방법을 통해 얻어진 분획도 본 발명의 난초 버섯균 발효 추출물에 포함된다. 또한, 본 발명의 난초 버섯균 발효 추출물은 감압 증류 및 동결건조 또는 분무 건조 등과 같은 추가적인 과정에 의해 분말 상태로 제조할 수 있다.The extract of the present invention includes not only the extract obtained by the above-mentioned extraction solvent, but also the extract obtained through ordinary purification process. For example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, various purification methods such as separation by various chromatography (made for separating by size, charge, hydrophobicity or affinity) Are also included in the fermentation extract of the orchid mushroom strain of the present invention. In addition, the fermented extract of orchid mushroom mycorrhizae of the present invention can be prepared in powder form by an additional process such as vacuum distillation, freeze drying, spray drying and the like.
다른 하나의 양태로서, 본 발명은 상기 방법에 의하여 제조된 난초 버섯균 발효 추출물에 관한 것이다.In another aspect, the present invention relates to a fermented extract of an orchid mushroom bacterium produced by the above method.
본 발명의 난초 버섯균 발효 추출물은 발효에 의하지 않은 난초 추출물에 비하여 세포 수준의 독성 농도가 높아 피부 자극이 작았으며(실험예 1), 멜라닌 종양세포에서의 멜라닌 생성 저해 효과가 현저하게 증가하였으며(실험예 2), 인간유래의 섬유아세포에서 콜라겐 생성량이 현저히 증가하였으며(실험예 3), 피부각질세포에서 자외선 조사에 의한 피부각질세포의 생존율이 현저하게 높았으며(실험예 4), 피부각질세포에서 자외선 조사에 의한 염증 반응이 현저하게 감소하였으며(실험예 5), 무모쥐에서 피부 장벽 기능의 회복 효과가 현저히 우수하였다(실험예 6). 따라서, 본 발명의 난초 버섯균 발효 추출물은 인간을 포함한 포유동물의 피부 보호 및 개선 효과, 피부 미백 효과, 피부 노화 및 주름의 예방 및 개선 효과, 자외선에 의한 피부 보호 및 피부의 염증 반응의 완화 효과, 및 피부 장벽 기능의 회복 효과가 우수하다. 상기 피부 보호 및 개선 효과는 외부로부터의 유해한 자극이나 대기 오염, 스트레스, 질병 등의 유해환경으로부터 피부를 보호하고, 피부의 정상적인 기능을 회복하는 것을 말하며, 특히 자외선에 의한 피부의 손상을 보호하고 피부의 염증 반응을 완화하는 것을 포함한다. 상기 피부 미백 효과는 피부 내의 멜라닌 합성을 저해함으로써 피부 내의 과도한 멜라닌 합성으로 인한 피부색이 어두워지고, 기미 또는 주근깨 등의 발생을 방지하는 것을 말한다. 상기 피부 노화 및 주름의 예방 및 개선 효과는 생체 내외의 유해환경에 의한 산화적 스트레스 및 이로 인한 피부내 세포의 기능 상실 등으로 인한 피부의 일정 부위 침하 현상(노화 및 주름)을 예방 및 개선하는 것을 말한다. 상기 피부 장벽 기능의 회복 효과는 손상된 지질 장벽의 회복을 자극시켜 정상적인 건강한 지질 장벽을 유지하도록 하는 것을 말한다. The fermented extract of orchid mushroom bacterium according to the present invention exhibited a small amount of skin irritation due to high toxic concentration at the cellular level as compared with the non-fermented orchid extract (Example 1), and the melanin formation inhibitory effect on melanin tumor cells was remarkably increased (Experimental Example 2), the amount of collagen production significantly increased in human-derived fibroblasts (Experimental Example 3), the survival rate of dermal keratinocytes by ultraviolet irradiation was remarkably high in dermal keratinocytes (Experimental Example 4) (Experimental Example 5), and the effect of restoring skin barrier function was remarkably excellent in hairless mouse (Experimental Example 6). Accordingly, the fermented extract of orchid mushroom mycorrhaphy of the present invention is effective for skin protection and improvement of mammals including humans, skin whitening effect, prevention and improvement of skin aging and wrinkles, skin protection by ultraviolet rays, , And the skin barrier function. The skin protection and improvement effect is to protect the skin from harmful environments such as harmful stimuli from the outside, air pollution, stress and diseases, and restore the normal function of the skin. In particular, it protects skin damage caused by ultraviolet rays, Lt; RTI ID = 0.0 > inflammatory < / RTI > The skin whitening effect refers to inhibiting the synthesis of melanin in the skin, thereby darkening the skin color due to excessive melanin synthesis in the skin, and preventing the occurrence of spots or freckles. The effect of preventing and improving skin aging and wrinkles is to prevent and improve certain settlement phenomena (aging and wrinkles) of the skin due to oxidative stress caused by harmful environment in and outside the living body, It says. The recovery effect of the skin barrier function is to stimulate the recovery of the damaged lipid barrier to maintain a normal healthy lipid barrier.
상기 본 발명의 난초 버섯균 발효 추출물에 사용된 난초 및 버섯균은 오래 전부터 사용해오던 재료로서 자체의 독성 및 부작용이 거의 없다. 따라서, 본 발명에 따른 난초 버섯균 발효 추출물은 독성에 대한 염려 없이 화장품 및 기능성 식품 등에 안전하게 사용할 수 있다.The orchid and mushroom bacteria used in the fermentation extract of the orchid mushroom bacteria of the present invention have been used for a long time and have little toxicity and side effects themselves. Therefore, the fermented extract of orchid mushroom bacteria according to the present invention can be safely used in cosmetics and functional foods without fear of toxicity.
하나의 구체화된 양태로서, 본 발명은 상기 난초 버섯균 발효 추출물을 포함하는 화장료 조성물을 제공한다.In one embodiment, the present invention provides a cosmetic composition comprising the fermented extract of orchid mushroom bacterium.
본 발명의 상기 화장료 조성물은 상기 난초 버섯균 발효 추출물을 전체 조성 물의 중량에서 0.0001 내지 50 중량%, 바람직하게는 0.01 내지 20 중량%로 포함한다. 난초 버섯균 발효 추출물의 함량이 0.0001 중량% 미만일 경우에는 뚜렷한 효과를 기대할 수 없다.The cosmetic composition of the present invention contains 0.0001 to 50% by weight, preferably 0.01 to 20% by weight of the fermented extract of orchid mushroom bacterium in terms of the weight of the whole composition. If the content of the fermented extract of orchid mushroom bacterium is less than 0.0001% by weight, a remarkable effect can not be expected.
본 발명의 화장료 조성물의 제형은 특별히 제한되지 않으며, 목적하는 바에 따라 적절히 선택할 수 있다. 예를 들어, 본 발명의 화장료 조성물은 유액, 크림, 화장수, 에센스, 팩, 젤, 파우더, 메이컵 베이스, 파운데이션, 로션, 연고, 패취, 미용액, 클렌징 폼, 클렌징 크림, 클렌징 워터, 바디 로션, 바디 크림, 바디 오일, 바디 젤, 바디 에센스, 샴푸, 린스, 바디 세정제, 비누, 염모제, 분무제, 오일젤 등의 제형으로 제조될 수 있다. The formulation of the cosmetic composition of the present invention is not particularly limited and can be appropriately selected according to the purpose. For example, the cosmetic composition of the present invention can be used as a cosmetic composition for oral use such as an emulsion, cream, lotion, essence, pack, gel, powder, makeup base, foundation, lotion, ointment, patch, essence, cleansing foam, cleansing cream, Body cream, body oil, body gel, body essence, shampoo, rinse, body cleanser, soap, hair dye, spray, oil gel and the like.
또한, 본 발명의 화장료 조성물은 각각의 제형에 상기 기재한 성분들 이외에 일반 피부화장료에 배합되는 유분, 물, 계면활성제, 보습제, 저급알코올, 증점제, 킬레이트제, 색소, 방부제, 또는 향료 등을 필요에 따라 적절히 배합하여 사용할 수 있다.In addition, the cosmetic composition of the present invention may contain other ingredients such as oil, water, a surfactant, a moisturizer, a lower alcohol, a thickener, a chelating agent, a coloring agent, an antiseptic, May be appropriately used in combination.
본 발명의 화장료 조성물에 포함된 난초 버섯균 발효 추출물은 상기에서 기재한 바와 같이, 인체에 무해할 뿐만 아니라 종래의 난초 추출물에 비하여 피부 보호 및 개선 효과, 피부 미백 효과, 피부 노화 및 주름의 예방 및 개선 효과, 피부 보호 및 피부의 염증 반응의 완화 효과, 및 피부 장벽 기능의 회복 효과 등이 우수하다. As described above, the fermented extract of orchid mushroom mycorrhiza contained in the cosmetic composition of the present invention is not only harmless to the human body, but also provides skin protection and improvement effects, skin whitening effect, prevention of skin aging and wrinkles, An effect of improvement, a skin protection and an effect of alleviating inflammation of the skin, and a recovery effect of skin barrier function.
본 발명의 난초 버섯균 발효 추출물 자체는 독성 및 부작용이 거의 없으므로 안전하게 화장료에 사용할 수 있다.Since the fermented extract of the mushroom mycelium of the present invention has little toxicity and side effects, it can be safely used in cosmetics.
하나의 구체화된 양태로서, 본 발명은 상기의 난초 버섯균 발효 추출물을 포함하는 기능성 식품에 관한 것이다.In one embodiment, the present invention relates to a functional food comprising the above fermented extract of orchid mushroom.
상기 식품으로 곡류, 감자류, 두류, 채소류, 과일류, 어패류, 육류, 우유류, 해조류 등의 천연식품; 또는 빵류, 과자류, 라면, 아이스크림 등의 가공식품; 또는 음료 등을 이용하여 제조할 수 있다.Such foods include natural foods such as cereals, potatoes, beans, vegetables, fruits, fish and shellfish, meat, milk, and seaweed; Processed foods such as bread, confectionery, ramen, and ice cream; Or beverages.
본 발명의 상기 난초 버섯균 발효 추출물은 피부 보호 및 개선 효과, 피부 미백 효과, 피부 노화 및 주름의 예방 및 개선 효과, 피부 보호 및 피부의 염증 반응의 완화 효과, 또는 피부 장벽 기능의 회복 효과를 목적으로 식품 또는 음료에 첨가될 수 있다. 이때, 식품 또는 음료 중의 상기 난초 버섯균 발효 추출물의 양은 일반적으로 식품 전체 중량의 0.01 내지 5 중량%로 가할 수 있으며, 음료는 100㎖를 기준으로 0.01 내지 2g, 바람직하게는 0.3 내지 1g의 비율로 가할 수 있다. The fermented extract of the orchid mushroom of the present invention is useful for skin protection and improvement effect, skin whitening effect, prevention and improvement of skin aging and wrinkles, skin protection and relieving skin inflammation reaction, or restoring skin barrier function To food or beverage. At this time, the amount of the fermentation extract of the orchid mushroom mycobacteria in the food or beverage may be generally 0.01 to 5% by weight of the total weight of the food, and the beverage may be added in a proportion of 0.01 to 2 g, preferably 0.3 to 1 g, Can be added.
본 발명의 난초 버섯균 발효 추출물이 첨가된 음료는 지시된 비율로 필수 성분으로서 상기 난초 버섯균 발효 추출물을 포함하는 것 외에는 액체 성분에는 특별한 제한점은 없으며 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다. 상기의 천연 탄수화물의 예는 포도당, 과당 등의 모노사카라이드; 말토스, 슈크로스 등의 디사카라이드; 덱스트린, 시클로덱스 트린 등과 같은 통상적인 당; 및 자일리톨, 소르비톨, 에리트리톨 등의 당알콜 등이 있다. 상기의 향미제로서 타우마틴, 스테비아 추출물(예를 들어 레바우디오시드 A, 글리시르히진 등) 등의 천연 향미제와 사카린, 아스파르탐 등의 합성 향미제를 유리하게 사용할 수 있다. 상기 천연 탄수화물의 비율은 본 발명의 천연 식물 추출물을 포함하는 피부보호 조성물 100㎖ 당 일반적으로 약 1 내지 20g, 바람직하게는 약 5 내지 12g이다.The beverage to which the fermented extract of the orchid mushroom mycorrhaphy of the present invention is added has no particular limitation on the liquid ingredient except that the fermented extract of the orchid mushroom bacterium is included as an essential ingredient in the indicated ratio. It is also possible to use various flavors or natural carbohydrates Or the like as an additional component. Examples of such natural carbohydrates include monosaccharides such as glucose and fructose; Disaccharides such as maltose, sucrose and the like; Conventional sugars such as dextrin, cyclodextrin and the like; And sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors such as tautatin and stevia extract (for example, rebaudioside A and glycyrrhizin) and synthetic flavors such as saccharin and aspartame may be advantageously used as the flavor agents. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the skin protection composition containing the natural plant extract of the present invention.
상기 외에 식품 및 음료는 여러 가지 영양제, 비타민, 광물(전해질), 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 증진제(치즈, 초콜릿 등), 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알콜, 탄산음료에 사용되는 탄산화제 등을 함유할 수 있다. 그 밖에 천연 과일 쥬스 및 과일 쥬스 음료 및 야채 음료의 제조를 위한 과육을 함유할 수 있다. 이러한 성분은 독립적으로 사용할 수 있다. 이러한 첨가제의 비율은 본 발명의 기능성 식품 100 중량% 당 0 내지 약 20 중량%의 범위에서 선택되는 것이 일반적이다.In addition to the above, foods and beverages may contain flavors such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate etc.), pectic acid and its salts, alginic acid and its salts, Organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. In addition, it may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components can be used independently. The proportion of such additives is generally selected in the range of 0 to about 20 wt% per 100 wt% of the functional food of the present invention.
본 발명의 난초 버섯균 발효 추출물은 식품에 직접 첨가하여 사용할 수도 있으나, 대상 식품을 직접 난초 버섯균 발효 추출물에 침지하거나 식품에 난초 버섯균 발효 추출물을 분무하는 방법으로 피부 보호 및 개선 효과, 피부 미백 효과, 피부 노화 및 주름의 예방 및 개선 효과, 피부 보호 및 피부의 염증 반응의 완화 효과, 및 피부 장벽 기능의 회복 효과를 얻을 수 있다.The fermented extract of orchid mushroom mycorrhizae of the present invention can be directly added to food, but it can be used by directly immersing the target food in fermented extract of orchid mushroom bacteria or spraying fermented extract of orchid mushroom bacteria with food, Effects of prevention and improvement of skin aging and wrinkles, skin protection and alleviation of skin inflammation reaction, and recovery of skin barrier function can be obtained.
본 발명의 난초 버섯균 발효 추출물 자체는 독성 및 부작용이 거의 없으므로 안전하게 식품에 사용할 수 있다.The fermented extract of orchid mushroom mycorrhiza of the present invention has little toxicity and side effects, and thus can be safely used for food.
본 발명의 난초의 버섯균 발효 추출물은 멜라닌 합성을 저해함으로써 피부 미백효과를 나타내고, 콜라겐 합성을 효과적으로 촉진하여 피부 노화를 방지하며, 주름 개선 효과 및 상처 치유 효과를 나타내며, 자외선에 대한 피부 세포 손상 방지 또는 염증반응을 억제함으로써 자외선에 대한 피부 보호 및 염증반응 완화 효과를 나타내며, 피부 장벽 기능 회복 강화 효과를 나타낸다. 따라서, 본 발명의 난초 버섯균 발효 추출물은 피부 보호 또는 개선용, 피부 미백용, 피부 노화 및 주름 예방 또는 개선용, 자외선에 대한 피부 보호 및 염증완화용, 피부 장벽 기능 회복 강화용으로 화장료 조성물 및 건강 기능성 식품에 유용하게 사용할 수 있다.The fermented extract of mushroom mycorrhiza of the present invention inhibits melanin synthesis, thereby exhibiting skin whitening effect, effectively promoting collagen synthesis, preventing skin aging, exhibiting wrinkle-improving effect and wound healing effect, preventing skin cell damage to ultraviolet rays Or by suppressing the inflammatory reaction, thereby exhibiting an effect of alleviating skin protection and inflammatory response against ultraviolet rays, and exhibiting an effect of enhancing restoration of skin barrier function. Therefore, the fermented extract of orchid mushroom mycorrhaphy of the present invention is useful as a cosmetic composition for skin protection or improvement, for skin whitening, for skin aging and wrinkle prevention or improvement, for protecting skin against ultraviolet rays and for relieving inflammation, It can be useful for health functional food.
이하에서는, 본 발명의 이해를 돕기 위하여 바람직한 실시예를 제시하나, 하기 실시예는 본 발명을 예시하는 것일 뿐 본 발명의 범위가 하기 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.
실시예Example 1: 난초 추출물의 제조 1: Preparation of orchid extract
시판되는 죽백란, 제주 한란, 석곡(장생란), 풍란을 구입(대한민국 대전시 유성구에 소재하는 대성화원)한 후, 이들을 각각 분쇄하고, 이들 각각의 분쇄물 200g을하정제수나 80% 메탄올 3L에 각각 넣고, 냉각콘덴서가 달린 환류추출기에서 3시간 동안 끓여 추출하였다. 그 다음, 100메쉬(mesh) 여과포로 여과한 후 잔사를 같은 방법으로 1회 더 추출하였다. 각각의 추출물을 합하여 상온에서 와트만 2번 여과지로 여과하여 불용성 물질을 제거하고 냉각콘덴서가 달린 증류장치에서 60℃로 감압농축한 후 동결 건조하여 하기 표 1과 같은 건조 중량을 얻었다. 하기 표 1은 추출용매에 따른 난초 100g당 추출물의 건조 중량을 나타낸 것이다.After that, 200 g of each of these crushed products was put into purified water or 3 L of 80% methanol, respectively. After that, And then boiled for 3 hours in a reflux extractor equipped with a condenser. After filtration through a 100-mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a filter paper of No. 2 Watt at room temperature to remove insoluble matter. The extract was concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, and then lyophilized to obtain a dry weight as shown in Table 1 below. Table 1 below shows the dry weight of the extract per 100 g of orchid according to the extraction solvent.
실시예Example 2: 난초의 2: of the orchid 버섯균Mushroom fungus 발효 추출물의 제조 Preparation of fermented extract
시판되는 죽백란, 제주 한란, 석곡(장생란), 풍란을 에서 구입(대한민국 대전시 유성구에 소재하는 대성화원)한 후, 이를 분쇄하고 121℃에서 15분간 습윤 멸균하였다. 이들 각각의 분쇄물 200g에 버섯균인 파에실로미세스 야포니카(Paecilomyces japonica)를 5 중량%로 첨가하고, 18 내지 25℃, 습도 60 내지 70%에서 60일간 발효시킨 후, 건조시킨 다음, 이를 분쇄하여 얻어진 분쇄물 100g씩을 80% 메탄올 3L에 각각 넣고 냉각콘덴서가 달린 환류추출기에서 3시간 끊여 추출하였다. 그 다음, 100메쉬 여과포로 여과한 후 잔사를 같은 방법으로 1회 더 추출하였다. 각각의 추출액을 합하여 상온에서 와트만 2번 여과지로 여과하여 불용성 물질을 제거한 후, 냉각콘덴서가 달린 증류장치에서 60℃로 감압농축한 후 동결건조하여 최종 난초 버섯균 발효 추출물을 수득하였다. 난초 버섯균 발효 추출물의 건조 중량을 하기 표 2에 나타내었다. It was crushed and moistly sterilized at 121 ° C for 15 minutes after it was purchased (commercially available bamboo grass, Jeju Hansan, Jagokgang, and wind chimes) (Daesung Garden, Yuseong-gu, Daejeon, Republic of Korea). 5% by weight of Paecilomyces japonica , a mushroom strain, was added to 200 g of each of these pulverized products, followed by fermentation at 18 to 25 DEG C and 60 to 70% of humidity for 60 days, followed by drying, 100 g of each of the obtained pulverized products was placed in 3 L of 80% methanol, and the mixture was extracted with a reflux condenser equipped with a condenser for 3 hours. After filtration with a 100 mesh filter cloth, the residue was extracted one more time by the same method. Each of the extracts was combined and filtered through a Watt filter paper No. 2 at room temperature to remove insoluble matter. The extract was concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a cooling condenser, and then lyophilized to obtain a final fermented extract of orchid mushroom bacteria. The dry weight of the orchid mushroom fermented extract is shown in Table 2 below.
실험예Experimental Example 1: 피부 자극 감소 효과 1: Reduces skin irritation
상기 실시예 1 및 2에서 제조한 난초 추출물 및 난초 버섯균 발효 추출물을 인간유래의 섬유아세포의 배양액에 첨가하여 세포 수준에서 독성을 비교 실험하여 피부 자극에 대한 실험을 유추하고자 하였다. 먼저 난초 추출물 및 난초 버섯균 발효 추출물들을 최종 농도가 0.1% 내지 0.0001%가 되도록 각각 섬유아세포 배지에 첨가하여 2일간 배양한 후, 배지를 제거하고 MTT Solution을 처리한 다음, 37℃에서 3시간 동안 배양하였다. 그 후, 12,000rpm에서 10분간 원심분리한 후, MTT 시약을 버리고 이소프로판올(Isopropanol)을 가하여 30분간 배양하여 세포를 깨뜨리고 분광광도계를 이용하여 570nm에서 흡광도를 측정하였다. 이들 측정한 결과에 따른 세포 독성 농도를 하기 표 3에 나타내었다. To evaluate the skin irritation by comparing the toxicity of the orchid extract and the orchid mushroom fermentation extract prepared in Examples 1 and 2 to the culture medium of human-derived fibroblasts at the cell level. First, the orchid extract and the orchid mushroom fermentation extracts were added to the fibroblast culture medium to a final concentration of 0.1% to 0.0001%, respectively. After culturing for 2 days, the medium was removed and the MTT solution was treated. Lt; / RTI > Then, after centrifugation at 12,000 rpm for 10 minutes, the MTT reagent was discarded, isopropanol was added, and the cells were incubated for 30 minutes, and the absorbance at 570 nm was measured using a spectrophotometer. The cytotoxic concentrations according to these measured results are shown in Table 3 below.
상기 표 3에 나타난 바와 같이, 본 발명의 난초 버섯균 발효 추출물(실시예 2)은 난초 추출물(실시예 1)과 비교하여 배양된 인간유래의 섬유아세포에 처리하였을 때 세포 독성이 낮아짐을 확인하였으며, 이는 일반적으로 세포 독성이 높은 물질이 피부자극도 높게 나타나는 것으로 보아 발효가 되면 피부 자극도 낮게 나올 것으로 유추할 수 있다.As shown in Table 3, it was confirmed that the fermented extract of the orchid mushroom strain of the present invention (Example 2) had lower cytotoxicity when treated with human-derived fibroblasts cultured in comparison with the orchid extract (Example 1) , It is generally considered that substances with high cytotoxicity show high skin irritation, so that it can be deduced that the skin irritation will be low when fermented.
실험예Experimental Example 2: 멜라닌 생성 저해효과 2: Melanin formation inhibitory effect
상기 실시예 2에서 수득한 난초 버섯균 발효 추출물을 마우스 유래 악성 멜라닌 종양 세포(B-16 mouse melanoma cell)의 배양액에 첨가하여 멜라닌 합성저해 효과를 실험하였다(Lotan R., Lotan D. Cancer Res. 40:3345-3350, 1980).The fermented extract of orchid mushroom bacteria obtained in Example 2 was added to a culture medium of mouse-derived malignant melanoma tumor cells (B-16 mouse melanoma cell) to examine the inhibitory effect on melanin synthesis (Lotan R., Lotan D. Cancer Res. 40: 3345-3350, 1980).
먼저, 상기 실시예 2에서 수득한 난초 버섯균 발효 추출물을 최종농도가 0.05%가 되도록 하여 하이드로퀴논 수용액과 함께 각각 악성 멜라닌 종양 세포(B-16 mouse melanoma cell)의 배지에 첨가하여 3일간 배양한 후, 트립신을 처리하여 세포들을 배양 용기로부터 떼어내 원심분리한 후 멜라닌을 추출하였다. 여기에 수산화나트륨 용액(1N농도) 1ml을 가하여 10분간 끓여 멜라닌을 녹이고 분광광도계를 이용하여 400nm에서의 흡광도를 측정하여 생성된 멜라닌의 양을 단위 세포수당 흡광도로 나타내는 방법으로 수행하였다. 상기 실험을 3회 이상 반복하여 측정하였으며, 대조군에 대한 상대적인 멜라닌 생성량을 저해율(%)로 계산하였고, 그 결과를 하기 표 4에 나타내었다. 또한 비교군으로 상기 실시예 1의 발효 과정을 거치지 않은 난초 추출물도 상기와 동일한 방법으로 측정하였다.First, the fermented extract of orchid mushroom bacteria obtained in Example 2 was added to a medium of B-16 mouse melanoma cells together with a hydroquinone aqueous solution to a final concentration of 0.05%, and cultured for 3 days After the cells were treated with trypsin, the cells were detached from the culture vessel and centrifuged to extract melanin. 1 ml of sodium hydroxide solution (1N concentration) was added thereto, and the mixture was boiled for 10 minutes to dissolve melanin. The absorbance at 400 nm was measured using a spectrophotometer, and the resulting amount of melanin was measured by absorbance per unit cell count. The above experiment was repeated three times or more and the amount of melanin produced relative to the control group was calculated as the inhibition rate (%). The results are shown in Table 4 below. As a control group, the orchid extract without the fermentation process of Example 1 was also measured in the same manner as described above.
상기 표 4에 나타난 바와 같이, 본 발명의 난초 버섯균 발효 추출물(실시예 2)은 일반 추출물인 실시예 1의 난초 추출물과 비교하여 배양된 악성 멜라닌 종양 세포에서 처리하였을 때 멜라닌 생성 저해가 현저하게 증가되었음을 확인할 수 있었다. As shown in Table 4, the fermented extract of Orchid mushroom mycobacterium of the present invention (Example 2) significantly inhibited melanin formation when treated with malignant melanoma tumor cells cultured in comparison with the ordinary extract of Example 1, , Respectively.
실험예Experimental Example 3: 콜라겐 합성 촉진효과 3: Promoting collagen synthesis
상기 실시예 1의 난초 추출물 및 실시예 2의 난초 버섯균 발효 추출물을 인간유래의 섬유아세포의 배양액에 첨가하여 세포수준에서 콜라겐 합성 효과를 실험하였다. 합성된 콜라겐의 측정은 PICP EIA kit(Procollagen Type Ⅰ C-Peptide Enzyme ImmunoAssay KIT)를 이용하여 정량하였다.The collagen synthesis effect at the cell level was examined by adding the orchid extract of Example 1 and the fermented extract of orchid mushroom bacteria of Example 2 to a culture solution of human fibroblasts. The synthesized collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay KIT).
상기 실시예 1의 난초 추출물 및 실시예 2의 난초 버섯균 발효 추출물을 최종농도가 0.05 %가 되도록 각각 인간유래의 섬유아세포(human fibroblast 세포)의 배양배지에 첨가하여 1 일간 배양한 후, 배양액을 취하여 PICP EIA Kit로 각 농도에서 콜라겐 합성 정도를 분광광도계를 이용하여 450 ㎚에서 측정하였다. 이때, 효과의 비교를 위하여 아무것도 첨가하지 않은 섬유아세포의 배양배지(대조군), 비타민 C를 최종농도 0.005 %가 되도록 첨가한 시료에 대하여 동일한 방법으로 콜라겐 합성 정도를 측정하였다.The orchid extract of Example 1 and the fermented extract of orchid mushroom bacteria of Example 2 were added to the culture medium of human fibroblast cells to a final concentration of 0.05% and cultured for 1 day. Then, The degree of collagen synthesis at each concentration was measured with a PICP EIA Kit at 450 nm using a spectrophotometer. At this time, for the comparison of the effects, the degree of collagen synthesis was measured by the same method for the culture medium of fibroblasts to which nothing was added (control group) and the sample to which vitamin C was added so as to have a final concentration of 0.005%.
콜라겐 생성량은 UV 흡광도로 측정하고, 콜라겐 생성 증가율은 대조군에 대한 상대적인 콜라겐 생성량의 비율로 계산하고 결과를 하기 표 5에 정리하였다.The amount of collagen production was measured by UV absorbance, and the rate of increase of collagen production was calculated as a ratio of the amount of collagen production relative to the control. The results are summarized in Table 5 below.
상기 표 5에 나타낸 바와 같이, 본 발명의 난초 버섯균 발효 추출물(실시예 2)은 대조군 및 실시예 1의 난초 추출물을 사용한 것 보다 인간유래의 섬유아세포에서 콜라겐 생성량이 현저히 증가되었으며, 전혀 정제되지 않은 상태임을 감안하면 기존에 알려진 콜라겐 합성 촉진물질인 비타민 C와 비교할 때 섬유아세포에서 매우 강력한 콜라겐 합성 촉진능을 가지고 있으므로, 피부주름 개선제나 상처치유제로 매우 유용하게 사용할 수 있음을 알 수 있었다.As shown in Table 5, the amount of collagen produced in human-derived fibroblasts was significantly higher than that of the control and the orchid extract of Example 1 in the fermented extract of the orchid mushroom strain of the present invention (Example 2) It can be said that it is very useful as a skin wrinkle remedy or a wound healing agent since it has a strong ability of stimulating collagen synthesis in fibroblasts as compared with vitamin C which is a known promoter of collagen synthesis.
실험예Experimental Example 4: 4: 지외선Earth Line 손상에 대한 피부 각질세포 보호효과 Protection of skin keratinocytes against damage
상기 실시예 1의 난초 추출물 및 실시예 2의 난초 버섯균 발효 추출물을 피부 각질세포주의 배양액에 첨가하여 세포수준에서 자외선에 대한 피부 각질세포 보호효과를 실험하였다. 25mJ/cm2의 UVB를 조사한 다음, 상기 실시예 1의 난초 추출물 및 실시예 2의 난초 버섯균 발효 추출물을 최종농도가 0.05 % 되도록 각각 피부각질세포의 배양배지에 첨가하여 1 일간 배양한 후, MTT분석법으로 세포 생존율을 측정하였다. 이때, 효과의 비교를 위하여 아무것도 첨가하지 않은 피부각질세포의 배양배지(대조군), WY14643 처리군을 최종농도 200uM가 되도록 첨가한 시료에 대하여 동일한 방법으로 세포 생존 정도를 측정하였다.The herbal extract of Example 1 and the fermented extract of orchid mushroom bacterium of Example 2 were added to the culture of keratinocyte cell line to examine the protective effect of keratinocytes against ultraviolet light at the cell level. After UVB irradiation at 25 mJ / cm 2 , the orchid extract of Example 1 and the fermentation extract of orchid mushroom bacteria of Example 2 were added to the culture medium of dermal keratinocytes to a final concentration of 0.05%, and the mixture was cultured for 1 day, Cell viability was measured by MTT assay. In order to compare the effects, the cell viability was measured in the same manner as in the culture medium (control group) in which keratinocytes were not added at all, and the WY14643-treated group was added to a final concentration of 200 uM.
세포 생존율은 UV 흡광도로 측정하고, 콜라겐 생성 증가율은 대조군에 대한 상대적인 생존률로 계산하고 결과를 하기 표 6에 나타내었다.Cell viability was measured by UV absorbance, and the rate of increase of collagen production was calculated as the relative survival rate for the control group. The results are shown in Table 6 below.
상기 표 6에 나타낸 바와 같이, 본 발명의 난초 버섯균 발효 추출물(실시예 2)은 대조군 및 난초 추출물을 사용한 것 보다 피부각질세포에서 자외선에 의한 피부각질세포의 생존율이 더 높음을 확인할 수 있었다.As shown in Table 6, it was confirmed that the extract of fermented orchid mushroom mycorrhizae of the present invention (Example 2) had a higher survival rate of dermal keratinocytes in the keratinocytes than the control and the orchid extract.
실험예Experimental Example 5: 피부 각질세포에서 5: In skin keratinocytes 지외선Earth Line 조사로 인한 염증반응 감소 효과 Reduction of inflammation response by irradiation
상기 실시예 1의 난초 추출물 및 실시예 2의 난초 버섯균 발효 추출물을 피부 각질세포주의 배양액에 첨가하여 세포수준에서 자외선에 대한 피부 각질세포의 염증반응 감소 효과를 실험하였다. 25mJ/cm2의 UVB를 조사하고, 상기 실시예 1 의 난초 추출물 및 실시예 2의 난초 버섯균 발효 추출물을 최종농도가 0.05% 되도록 각각 피부각질세포의 배양배지에 첨가하여 1 일간 배양한 후, 배양액을 수거하여 염증성 사이토카인으로 알려진 TNF-a의 양을 ELISA로 측정하였다. 이때, 효과의 비교를 위하여 아무것도 첨가하지 않은 피부각질세포의 배양배지(대조군), WY14643처리군 을 최종농도 200uM가 되도록 첨가한 시료에 대하여 동일한 방법으로 TNF-a 분비량을 측정하였다. TNF-a 분비량 증가율은 대조군에 대한 상대적인 분비량으로 계산하고 결과를 하기 표 7에 정리하였다.The effect of decreasing the inflammatory response of keratinocytes to ultraviolet light at the cell level was examined by adding the fermented extract of the orchid extract of Example 1 and the extract of orchid mushroom bacteria of Example 2 to the culture of keratinocyte cell line. UVB of 25 mJ / cm 2 was irradiated, and the orchid extract of Example 1 and the fermentation extract of orchid mushroom bacteria of Example 2 were added to a culture medium of dermal keratinocyte to a final concentration of 0.05% and cultured for 1 day, The culture was collected and the amount of TNF-a, known as inflammatory cytokine, was measured by ELISA. At this time, for the comparison of the effects, the amount of TNF-α secretion was measured in the same manner as in the culture medium of the keratinocytes without any addition (control group) and the sample to which the WY14643 treatment group was added to a final concentration of 200 μM. The increase rate of TNF-a secretion amount was calculated as a relative secretion amount to the control group, and the results are summarized in Table 7 below.
상기 표 7에 나타낸 바와 같이, 본 발명의 난초 버섯균 발효 추출물(실시예 2)은 대조군 및 난초 추출물을 사용한 것 보다 피부각질세포에서 자외선에 의한 TNF-a 분비량은 더 낮아지는 것을 확인할 수 있었다. As shown in Table 7, it was confirmed that the extract of fermented orchid mushroom mycorrhiza of the present invention (Example 2) had lower amount of TNF-a secreted by ultraviolet light in keratinocytes than the control and the orchid extract.
실험예Experimental Example 6: 피부장벽 기능 회복 강화 효과 6: Enhancement of skin barrier function recovery
상기 실시예 1의 난초 추출물 및 실시예 2의 난초 버섯균 발효 추출물을 피부 장벽 기능 회복 강화 효과를 알아보기 위하여 무모쥐에서 다음과 같이 실험하였다.To investigate the effect of the extract of the orchid extract of Example 1 and the extract of fermentation of orchid mushroom bacterium of Example 2 on skin barrier function recovery, the following experiments were carried out in hairless mice.
먼저, 생후 8-12주령의 무모유도생쥐의 등 양쪽의 수분 증발량을 TEWL 장치로 측정한 후, 동일한 위치에 스카치 테이프를 붙였다 떼어 붙이는 것을 15-20회 정도 반복하면서 수분 증발량이 40-50g/hm2정도에 이를 때까지 반복하였다. 이후, 2%의 상기 실시예 1 및 2에서 수득된 각 난초 추출물 및 난초 버섯균 발효 추출물을 각각 손상직후, 6시간 후, 12시간 후에 도포하였고, 다른 한쪽 등에는 아무것도 도포하지 않은 것을 대조군으로 하여 시간의 경과에 따라 수분증발량을 측정하여 피부 장벽의 복원 정도를 확인하였다. 그 결과를 하기 표 8에 나타내었다.First, the water evaporation of both sides of 8-12 weeks old uninflamed mice was measured with a TEWL apparatus, and a Scotch tape was attached and peeled at the same position repeated 15-20 times, and the moisture evaporation was 40-50 g / hm 2 . Then, 2% of each of the orchid extracts obtained in Examples 1 and 2 and the fermentation extract of orchid mushroom bacteria were applied immediately after the injury, 6 hours, and 12 hours later, and the other one was not applied to the other The amount of moisture evaporation was measured over time to confirm the degree of restoration of the skin barrier. The results are shown in Table 8 below.
상기 표 8에 나타낸 바와 같이, 본 발명의 난초 버섯균 발효 추출물(실시예 2)은 대조군 및 난초 추출물을 사용한 것 보다 피부 장벽의 복원에 따른 수분증발량이 낮아지는 것을 확인할 수 있었다. As shown in Table 8, it was confirmed that the extract of fermented orchid mushroom mycorrhizae of the present invention (Example 2) had lower moisture evaporation amount due to the restoration of the skin barrier than the control or the orchid extract.
제조예Manufacturing example 1: 에센스 제조 1: Essence manufacturing
자기 유화형 모노스테아린산
밀납
스쿠알란
이소세틸옥타노에이트
디메틸실록산
모노스테아린산소르비탄
모노스테아린산폴리에틸렌글리콜
글리세린
프로필렌글리콜
카르복시폴리머
트리에탄올아민
향료
색소
난초 발효추출물
메칠 파라벤
프로필 파라벤
정제수Cetostearyl alcohol
Self emulsifying monostearic acid
Wax
Squalane
Isocetyl octanoate
Dimethylsiloxane
Sorbitan monostearate
Polyethylene glycol monostearate
glycerin
Propylene glycol
Carboxy polymer
Triethanolamine
Spices
Pigment
Orchid fermented extract
Methylparaben
Propylparaben
Purified water
1.0
0.5
6.0
3.0
0.3
0.5
8.0
5.0
0.2
0.2
0.22
적량
적량
10.0
2
1
to 1001.0
1.0
0.5
6.0
3.0
0.3
0.5
8.0
5.0
0.2
0.2
0.22
Suitable amount
Suitable amount
10.0
2
One
to 100
상기 실시예 2의 난초 버섯균 발효 추출물을 이용하여 상기 표 9의 처방으로 에센스를 제조하였다(단위는 중량%이다).Using the fermented extract of orchid mushroom bacteria of Example 2, the essence was prepared by the prescription of the above Table 9 (unit is weight%).
제조예Manufacturing example 2: 로션 제조 2: Lotion manufacturing
자기 유화형 모노스테아린산
밀납
스테아린산
유동파라핀
스쿠알란
미카데미아오일
이소세틸옥타노에이트
디메틸실록산
모노스테아린산소르비탄
모노스테아린산폴리에틸렌글리콜
글리세린
프로필렌글리콜
베타민
카르복시폴리머
트리에탄올아민
향료
색소
난초 발효추출물
메칠 파라벤
프로필 파라벤
정제수Cetostearyl alcohol
Self emulsifying monostearic acid
Wax
Stearic acid
Liquid paraffin
Squalane
Mikademia oil
Isocetyl octanoate
Dimethylsiloxane
Sorbitan monostearate
Polyethylene glycol monostearate
glycerin
Propylene glycol
Betamin
Carboxy polymer
Triethanolamine
Spices
Pigment
Orchid fermented extract
Methylparaben
Propylparaben
Purified water
1.0
0.5
0.5
8.0
4.0
2.0
2.0
0.3
0.5
1.2
4.0
4.0
4.0
0.12
0.15
적량
적량
5
0.2
0.1
to 1000.8
1.0
0.5
0.5
8.0
4.0
2.0
2.0
0.3
0.5
1.2
4.0
4.0
4.0
0.12
0.15
Suitable amount
Suitable amount
5
0.2
0.1
to 100
상기 실시예 2의 난초 버섯균 발효 추출물을 이용하여 상기 표 10의 처방으로 로션을 제조하였다(단위는 중량%이다).Using the fermented extract of orchid mushroom bacterium of Example 2, a lotion was prepared according to the prescription of Table 10 (unit: wt%).
제조예Manufacturing example 3: 크림 제조 3: Cream production
자기 유화형 모노스테아린산
친유형 모노스테아린산
밀납
유동파라핀
스쿠알란
이소세틸옥타노에이트
정제 호호바유
디메틸실록산
모노스테아린산소르비탄
모노스테아린산폴리에틸렌글리콜
글리세린
프로필렌글리콜
베타인
산탄검
트리에탄올아민
향료
색소
난초 발효추출물
메칠 파라벤
프로필 파라벤
정제수Cetostearyl alcohol
Self emulsifying monostearic acid
Chin type monostearate
Wax
Liquid paraffin
Squalane
Isocetyl octanoate
Refined jojoba oil
Dimethylsiloxane
Sorbitan monostearate
Polyethylene glycol monostearate
glycerin
Propylene glycol
Betaine
Xanthan gum
Triethanolamine
Spices
Pigment
Orchid fermented extract
Methylparaben
Propylparaben
Purified water
1.5
1.5
0.5
6.0
8.0
4.0
4.0
0.3
1.0
1.2
8.0
4.0
4.0
0.06
0.10
적량
적량
5
0.25
0.1
to 1003.0
1.5
1.5
0.5
6.0
8.0
4.0
4.0
0.3
1.0
1.2
8.0
4.0
4.0
0.06
0.10
Suitable amount
Suitable amount
5
0.25
0.1
to 100
상기 실시예 2의 난초 버섯균 발효 추출물을 이용하여 상기 표 11의 처방으로 크림을 제조하였다(단위는 중량%이다).Using the fermented extract of orchid mushroom bacterium of Example 2, the cream was prepared in the prescription of Table 11 (unit is weight%).
제조예Manufacturing example 4: 젤 제조 4: Gel manufacturing
프로필렌글리콜
에탄올
폴리옥시에틸렌경화 피마자유
카르복시폴리머
트리에탄올아민
향료
색소
난초 발효추출물
메칠 파라벤
프로필 파라벤
정제수glycerin
Propylene glycol
ethanol
Polyoxyethylene hardened castor oil
Carboxy polymer
Triethanolamine
Spices
Pigment
Orchid fermented extract
Methylparaben
Propylparaben
Purified water
5.0
10
0.2
0.30
0.30
적량
적량
10
0.25
0.1
to 1005.0
5.0
10
0.2
0.30
0.30
Suitable amount
Suitable amount
10
0.25
0.1
to 100
상기 실시예 2의 난초 버섯균 발효 추출물을 이용하여 상기 표 12의 처방으로 젤을 제조하였다(단위는 중량%이다).Using the fermented extract of orchid mushroom bacterium of Example 2, the gel was prepared in the prescription of Table 12 (unit: wt%).
제조예Manufacturing example 5: 연고 제조 5: Ointment manufacturing
자기 유화형 모노스테아린산
스테아린산
밀납
스쿠알란
모노스테아린글리세린
모노스테아린산소르비탄
폴리솔베이트 80
글리세린
프로필렌 글리콜
향료
색소
난초 발효 추출물
메칠 파라벤
프로필 파라벤
정제수Cetostearyl alcohol
Self emulsifying monostearic acid
Stearic acid
Wax
Squalane
Monostearin glycerin
Sorbitan monostearate
Polysorbate 80
glycerin
Propylene glycol
Spices
Pigment
Orchid fermented extract
Methylparaben
Propylparaben
Purified water
2.0
1.0
4.0
8.0
4.0
2.0
3.0
5.0
4.0
적량
적량
10
0.25
0.1
to 1002.0
2.0
1.0
4.0
8.0
4.0
2.0
3.0
5.0
4.0
Suitable amount
Suitable amount
10
0.25
0.1
to 100
상기 실시예 2의 난초 버섯균 발효 추출물을 이용하여 상기 표 13의 처방으로 연고를 제조하였다(단위는 중량%이다).The oyster mushroom fermented extract of Example 2 was used to prepare an ointment by the prescription of Table 13 (unit: wt%).
제조예Manufacturing example 6. 식품의 제조 6. Manufacturing of food
본 발명의 조성물을 포함하는 식품들을 다음과 같이 제조하였다.Foods comprising the composition of the present invention were prepared as follows.
1. 조리용 양념의 제조1. Preparation of cooking seasoning
본 발명의 상기 실시예 2의 난초 버섯균 발효 추출물을 1~15 중량%로 통상적인 조리용 양념에 첨가하여 제조하였다.The fermented extract of orchid mushroom bacterium of Example 2 of the present invention was prepared by adding 1 to 15% by weight of the extract to a conventional cooking seasoning.
2. 토마토 케찹 및 소스의 제조2. Manufacture of tomato ketchup and sauce
본 발명의 상기 실시예 2의 난초 버섯균 발효 추출물을 0.2~1.0 중량%로 토마토 케찹 또는 소스에 첨가하여 제조하였다.The fermented extract of orchid mushroom bacterium of Example 2 of the present invention was prepared by adding 0.2-1.0 wt% of the extract to tomato ketchup or sauce.
3. 밀가루 식품의 제조3. Manufacture of flour food
본 발명의 상기 실시예 2의 난초 버섯균 발효 추출물을 0.5~5.0 중량%로 밀가루에 첨가하고, 이 혼합물을 이용하여 빵, 케이크, 쿠키, 크래커 및 면류를 제조하였다.The fermented extract of orchid mushroom bacterium of Example 2 of the present invention was added to wheat flour at 0.5-5.0 wt%, and bread, cake, cookies, crackers and noodles were prepared using this mixture.
4. 스프 및 육즙(gravies)의 제조4. Manufacture of soups and gravies
본 발명의 상기 실시예 2의 난초 버섯균 발효 추출물을 0.1~5.0 중량%로 스프 및 육즙에 첨가하여 육가공 제품, 면류의 수프 및 육즙을 제조하였다.The fermented extract of orchid mushroom bacterium of Example 2 of the present invention was added to soups and juices at 0.1 to 5.0 wt% to prepare meat products, noodle soups and juices.
5. 그라운드 비프(ground beef)의 제조5. Manufacture of ground beef
본 발명의 상기 실시예 2의 난초 버섯균 발효 추출물을 10 중량%로 그라운드 비프에 첨가하여 제조하였다.The fermented extract of the mushroom mycelium of Example 2 of the present invention was prepared by adding 10% by weight of the extract to the ground beef.
6. 유제품(dairy products)의 제조6. Manufacture of dairy products
본 발명의 상기 실시예 2의 난초 버섯균 발효 추출물을 5~10 중량%로 우유에 첨가하고, 상기 우유를 이용하여 버터 및 아이스크림과 같은 다양한 유제품을 제조하였다.The fermented extract of orchid mushroom bacterium of Example 2 of the present invention was added to milk in an amount of 5 to 10% by weight and various dairy products such as butter and ice cream were prepared using the milk.
7. 선식의 제조7. Manufacturing of wires
현미, 보리, 찹쌀, 율무를 공지의 방법으로 알파화시켜 건조시킨 것을 배전한 후 분쇄기로 입도 60메쉬의 분말로 제조하였다. Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.
검정콩, 검정깨, 들깨도 공지의 방법으로 쪄서 건조시킨 것을 배전한 후 분쇄기로 입도 60메쉬의 분말로 제조하였다.Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a conventional method, and then they were prepared into powder having a particle size of 60 mesh by a pulverizer.
본 발명의 상기 실시예 2의 난초 버섯균 발효 추출물을 진공 농축기에서 감압 및 농축하고, 분무 또는 열풍건조기로 건조하여 얻은 건조물을 분쇄기로 입도 60메쉬로 분쇄하여 건조분말을 얻었다.The fermented extract of orchid mushroom bacteria of Example 2 of the present invention was reduced in pressure and concentrated in a vacuum concentrator, dried with a spray or a hot-air drier, and pulverized to a size of 60 mesh with a pulverizer to obtain a dry powder.
상기에서 제조한 곡물류, 종실류 및 난초 발효 추출물의 건조분말을 다음의 비율로 배합하여 제조하였다.The above-prepared cereal grains, seeds and dried powder of the orchid fermentation extract were blended in the following proportions.
곡물류(현미 30중량%, 율무 15중량%, 보리 20중량%)Cereals (30% by weight of brown rice, 15% by weight of yulmu, 20% by weight of barley)
종실류(검정콩 8중량%, 들깨 7중량%, 검정깨 7중량%)Seeds (black bean 8 wt%, perilla 7 wt%, black sesame 7 wt%)
실시예 2의 난초 버섯균 발효 추출물의 건조분말(3 중량%)The dried powder (3% by weight) of the fermented extract of orchid mushroom bacterium of Example 2
영지(0.5중량%)Ganoderma lucidum (0.5% by weight)
지황(0.5중량%)(0.5% by weight)
제조예Manufacturing example 7. 음료의 제조 7. Manufacturing of beverages
1. 탄산음료의 제조1. Manufacture of carbonated beverages
설탕 5~10%, 구연산 0.05~0.3%, 카라멜 0.005~0.2%, 비타민 C 0.1~1%의 첨가물을 혼합하고, 여기에 79~94%의 정제수를 섞어서 시럽을 만들고, 상기 시럽을 85~98℃에서 20~180초간 살균하여 냉각수와 1: 4의 비율로 혼합한 다음 탄산가스를 0.5~0.82%를 주입하여 본 발명의 상기 실시예 2의 난초 버섯균 발효 추출물을 함유하는 탄산음료를 제조하였다.The syrup is prepared by mixing additives of 5 to 10% of sugar, 0.05 to 0.3% of citric acid, 0.005 to 0.2% of caramel and 0.1 to 1% of vitamin C and mixing 79 to 94% of purified water thereto, ° C for 20 to 180 seconds and mixed with cooling water at a ratio of 1: 4, followed by the addition of 0.5 to 0.82% carbon dioxide gas to prepare a carbonated beverage containing the fermented extract of orchid mushroom bacteria of Example 2 of the present invention .
2. 건강음료의 제조2. Manufacture of health drinks
액상과당(0.5%), 올리고당(2%), 설탕(2%), 식염(0.5%), 물(75%)과 같은 부재료와 본 발명의 상기 실시예 2의 난초 버섯균 발효 추출물을 균질하게 배합하여 순간 살균을 한 후, 이를 유리병, 패트병 등 소포장 용기에 포장하여 건강음료를 제조하였다.The fermented extract of the orchid mushroom strain of Example 2 of the present invention was uniformly mixed with a mixture of liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5% And then sterilized in an instant, and then packaged in small containers such as glass bottles and plastic bottles to produce health drinks.
3. 야채쥬스의 제조3. Manufacture of vegetable juice
본 발명의 상기 실시예 2의 난초 버섯균 발효 추출물 5g을 토마토 또는 당근 쥬스 1,000㎖에 가하여 야채쥬스를 제조하였다.5 g of the fermented extract of orchid mushroom bacteria of Example 2 of the present invention was added to 1,000 ml of tomato or carrot juice to prepare a vegetable juice.
4. 과일쥬스의 제조4. Manufacture of fruit juice
본 발명의 상기 실시예 2의 난초 버섯균 발효 추출물 1g을 사과 또는 포도 쥬스 1,000㎖에 가하여 과일쥬스를 제조하였다.1 g of the fermented extract of orchid mushroom bacteria of Example 2 of the present invention was added to 1,000 ml of apple or grape juice to prepare fruit juice.
상기 조성비는 조제예에 의해 한정된 것이 아니고 본 발명의 기술적인 면에서 벗어나지 않는 범위 내에서 여러 가지 치환, 변형 및 변경이 가능하다.The composition ratio is not limited to the preparation examples, and various substitutions, modifications, and changes may be made without departing from the technical scope of the present invention.
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KR101033921B1 (en) * | 2009-06-04 | 2011-05-11 | 웅진코웨이주식회사 | Composition for reducing irritation and inflammation in skin |
KR101100678B1 (en) * | 2009-06-04 | 2012-01-03 | 웅진코웨이주식회사 | Cosmetic Composition comprising plant extracts |
CN103957885A (en) | 2011-09-14 | 2014-07-30 | 奈夫利公司 | Herbal skin care composition |
CA2859838A1 (en) | 2011-12-23 | 2013-06-27 | Nefeli Corp. | Astragalus seed extract solution and its use for treating skin disorders and conditions |
CN103431127A (en) * | 2013-09-25 | 2013-12-11 | 福建古农堂生物科技有限公司 | Dendrobe tea and preparation method thereof |
CN104188038A (en) * | 2014-07-29 | 2014-12-10 | 汤兴华 | Dendrobe electuary with function of beautifying |
CN104305175A (en) * | 2014-09-26 | 2015-01-28 | 安徽新津铁皮石斛开发有限公司 | Desiccated coconut-haw flavored dendrobium candidum superfine granule and preparation method thereof |
CN104305174A (en) * | 2014-09-26 | 2015-01-28 | 安徽新津铁皮石斛开发有限公司 | Lotus-mung bean flavored dendrobium candidum superfine granule and preparation method thereof |
CN104207131A (en) * | 2014-09-26 | 2014-12-17 | 安徽新津铁皮石斛开发有限公司 | Crab milk flavor dendrobium officinale ultrafine powder beverage and preparation method thereof |
CN105077143A (en) * | 2015-09-17 | 2015-11-25 | 合肥微信片农业科技有限公司 | Condiment containing dendrobe |
CN105919897A (en) * | 2016-06-21 | 2016-09-07 | 陆锦满 | Body cleaning liquid prepared from dendrobium officinale extracting solution |
CN105900695B (en) * | 2016-06-28 | 2019-03-01 | 广西壮仁堂生物科技有限公司 | Cordyceps mycelia is prepared by culture medium of dendrobium candidum |
KR101945868B1 (en) * | 2016-11-14 | 2019-02-11 | 대한민국 | Neofinetia falcata extracts, preparing method of the same, and cosmetic composition and pharmaceutical composition including the same |
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