KR20150030090A - Composition for skin whitening containing Panax ginseng extract and Green tea extract - Google Patents

Abstract

The present invention relates to a skin whitening composition containing a panax ginseng extract and a green tea extract, and more specifically, to a composition which provides a synergy effect by mixing a panax ginseng extract and a green tea extract, shows excellent skin whitening effect than when using the components respectively, alleviates pigmentation such as freckle or the like or darkness of skin, and makes skin color uniform and bright.

Description

Technical Field [0001] The present invention relates to a skin whitening composition containing a ginseng extract and a green tea extract. [0002] Composition for skin whitening containing Panax ginseng extract and Green tea extract [

The present invention relates to a whitening composition containing a ginseng extract and a green tea extract. More specifically, the present invention can provide a synergistic effect by mixing a ginseng extract and a green tea extract. Thus, The present invention relates to a composition which improves pigmentation symptoms such as spots and freckles or dullness of the skin and makes the skin color uniform and bright.

Human skin color is determined by various factors such as the activity of melanocyte making melanocyte, the distribution of blood vessels, the thickness of skin, and the presence or absence of pigment inside and outside the body, such as carotenoids and bilirubin. The most important factor is melanin, a melanin produced by various enzymes such as tyrosinase in melanocytes. The formation of melanin pigment is affected by environmental factors such as genetic factors, hormonal secretion, physiological factors such as stress, and ultraviolet irradiation. Melanin is present in the skin and protects the body from ultraviolet rays, but it is known that melanin is overproduced, thereby promoting pigmentation and aging of the skin and leading to skin cancer induction. (Ascorbic acid, kojic adcid, arbutin, hudroquinone) have been previously used to treat or relieve excessive melanin pigmentation caused by skin pigmentation abnormalities and ultraviolet exposure. Glutathione or a derivative thereof and a substance having inhibitory activity against tyrosinase have been used in cosmetics or medicines in combination with insufficient whitening effect, safety problems to the skin, Its use is restricted due to stability problems and so on.

Accordingly, the inventors of the present invention found that, when the ginseng extract and the green tea extract are simultaneously used, they provide a superior skin whitening effect as compared with the case of using each of these substances by a synergistic effect, .

Accordingly, it is an object of the present invention to provide a composition showing excellent skin whitening effect by using ginseng extract and green tea extract simultaneously.

In order to achieve the above object, the present invention provides a whitening skin external composition or pharmaceutical composition containing ginseng extract and green tea extract as an active ingredient.

The composition of the present invention provides an excellent skin whitening effect by containing ginseng extract and green tea extract, thereby reducing pigmentation symptoms such as spots and freckles, improving skin dullness, and making skin color uniform and bright.

The composition of the present invention contains an extract of ginseng and an extract of green tea as an active ingredient, thereby providing an excellent skin whitening effect.

The ginseng (Panax ginseng C.A. Meyer) used in the present invention is a plant belonging to the genus Omega and Ginseng. In the present invention, ginseng can be used regardless of its kind and year, and both dried and not dried ginseng can be used. In the present invention, ginseng can be used without limitation in the site, and specifically, roots of ginseng can be used.

The green tea used in the present invention is not limited to the type and number of days of cultivation, and both dried and non-dried green tea can be used. In the present invention, the green tea can be used without limitation in the area, and specifically, the leaves of green tea can be used.

The ginseng extract and green tea extract used in the present invention can be prepared by a method known in the art, and the method is not particularly limited. Specifically, ginseng extract or green tea extract can be prepared with water or an organic solvent. The organic solvent used in the present invention may be selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate, chloroform and a mixed solvent of these organic solvents and water, preferably 80% ethanol. At this time, the extraction temperature is preferably 10 to 80 ° C, and the extraction can be performed for 3 to 24 hours. If the extraction temperature and the extraction time are exceeded, the extraction efficiency may be deteriorated or the component may be changed.

The composition of the present invention may contain the ginseng extract and the green tea extract in an amount of 0.001 to 10 wt%, preferably 0.005 to 9.5 wt%, based on the total weight of the composition, 0.09 to 7.5 wt.%, 0.1 to 6.5 wt.%, 0.3 to 6 wt.%, 0.5 to 5.5 wt.%, Or 0.7 By weight to 5% by weight.

In the present invention, the ginseng extract and the green tea extract are mixed in a weight ratio of 0.1 to 10: 1. Preferably, ginseng extract and green tea extract are mixed in a weight ratio of 0.5 to 9.5: 1, 1 to 9: 1, or 1.5 to 8.5: 1, in order to maximize the synergistic effect of the two substances in combination .

The composition of the present invention may be formulated as an external preparation for skin, in particular as a cosmetic composition, and may be formulated containing a cosmetically or dermatologically acceptable medium or base. In addition, the composition of the present invention may be provided in any form suitable for topical application, for example, as a solution, an emulsion obtained by dispersing an oil phase in water phase, an emulsion obtained by dispersing water phase in water phase, a suspension, a solid, a gel, Pastes, foams, or aerosol compositions. Compositions of such formulations may be prepared according to conventional methods in the art.

In addition, the composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, to the extent that the main effect is not impaired. The composition according to the present invention may further comprise a humectant, an emollient, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic or inorganic pigment, a perfume, a cold agent or a limiting agent. The compounding amount of the above components can be easily selected by a person skilled in the art within the range not impairing the object and effect of the present invention, and the amount thereof may be from 0.01 to 5% by weight, specifically from 0.01 to 3% by weight based on the total weight of the composition .

In addition, the composition of the present invention can be formulated as a pharmaceutical composition. When the composition according to the present invention is applied to medicines, it may be formulated into oral, parenteral or parenteral dosage forms in solid, semi-solid or liquid form by adding an inorganic or organic carrier compatible with the active ingredient used in the present invention , The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like.

Examples of the formulations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. In addition, the formulations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. The composition according to the present invention can be easily formulated into an active ingredient by carrying out the composition according to a conventional method. In this case, a surfactant, excipient, coloring agent, spice, preservative, stabilizer, buffer, suspending agent, have.

The dosage of the active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, sex, body weight, pathological condition and severity of the subject to be treated, route of administration, or judgment of the prescriber. Determination of the appropriate dose based on these factors is well within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / / Day, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[Reference Example 1] Preparation of ginseng extract

1 kg of dried white ginseng was pulverized with a blender, and 5 liters of purified water was added thereto, followed by stirring at 38 to 42 ° C in hot water for 24 hours, followed by immersion for one day at 15 ° C. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 270 g of the extract.

[Reference Example 2] Preparation of green tea extract

1 kg of first processed green tea leaves were pulverized with a blender, 5 liters of purified water was added thereto, and the mixture was stirred for 24 hours at 38 to 42 ° C in hot water, and then immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 290 g of the extract.

[Example 1] Preparation of a mixture of ginseng extract and green tea extract

The ginseng extract prepared in Reference Example 1 and the green tea extract prepared in Reference Example 2 were mixed at a ratio of 1: 3.

[Example 2] Preparation of a mixture of ginseng extract and green tea extract

The ginseng extract prepared in Reference Example 1 and the green tea extract prepared in Reference Example 2 were mixed at a ratio of 1: 1.

[Example 3] Preparation of a mixture of ginseng extract and green tea extract

The ginseng extract prepared in Reference Example 1 and the green tea extract prepared in Reference Example 2 were mixed at a ratio of 3: 1.

Test Example 1: Effect of tyrosinase inhibition

The tyrosinase enzyme was extracted from Mushroom and was obtained from Sigma (SIGMA). First, the substrate, tyrosine, is dissolved in distilled water to make a 0.3 mg / ml solution. The solution is put into a test tube in an amount of 1.0 ml. Then, 1.0 ml of potassium phosphate buffer solution (0.1 mol concentration, pH 6.8) and 0.7 ml of distilled water .

0.2 ml of the sample solution prepared by mixing the ginseng extract and the green tea extract of Examples 1 to 3 or the ginseng extract and the green tea extract of Reference Examples 1 and 2 at appropriate concentrations in the respective ethanol solutions was added to the reaction solution, The reaction was carried out in a thermostat for 10 minutes. As a negative control, 0.2 ml of a solvent was used instead of each sample solution, and ascorbic acid was used as a positive control. 0.1 ml of tyrosinase solution of 2500 units / ml was added to the reaction solution, followed by reaction at 37 ° C for 10 minutes. The reaction tube was immersed in ice water to quench the reaction, and the absorbance at 475 nm was measured with a photoelectron spectrometer. The results are shown in Table 1 below. Each tyrosinase inhibitory effect was calculated by the following equation (1).

Test substance Inhibition rate of tyrosinase (%) Control group (no addition) 0 Ascorbic acid 52 Example 1 48 Example 2 48 Example 3 46 Reference Example 1 41 Reference Example 2 39

In the results of Table 1 above, Reference Examples 1 and 2 using green tea extracts of ginseng extract showed inhibitory effects of tyrosinase, but in the case of Examples 1 to 3 in which these extracts were mixed, 1 < / RTI > and < RTI ID = 0.0 > 2, < / RTI >

[Test Example 2] Inhibitory effect on melanin formation by B16 / F10 melanoma cells

Using a mixture of the ginseng extract and the green tea extract of Examples 1 to 3, or each of the ginseng extract and green tea extract of Reference Examples 1 and 2 in an amount of 0.1% by weight as a test substance, B16 / F10 melanoma cells (Korean Cell Line Bank), cultured for 3 days, the culture solution was removed, washed with PBS, and the cells were dissolved with 1N NaOH and absorbance was measured at 405 nm. Cells to which the test substance was not added were used as a control group, and the melanin formation inhibition degree of each test substance was measured by comparing with the melanin content in the control group. The inhibition rate of melanin production was calculated according to Equation (2), and the results are shown in Table 2.

Test substance Melanin inhibition rate (%) Control group (no addition) 0 Kojic acid 53 Example 1 51 Example 2 53 Example 3 54 Reference Example 1 44 Reference Example 2 45

In the results of Table 2 above, Reference Examples 1 and 2 using green tea extracts of ginseng extract showed inhibitory effects on melanogenesis, but in the case of Examples 1 to 3 in which these extracts were mixed, It can be seen that a remarkably high melanin production inhibitory effect is exhibited as compared with Examples 1 and 2, and an excellent whitening effect can be provided.

[Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3]

Nutritive creams were prepared in a conventional manner according to the composition of Table 3 below (unit: wt%).

Compounding ingredient Formulation Example 1 Formulation Example 2 Formulation Example 3 compare
Formulation Example 1 compare
Formulation Example 2 compare
Formulation Example 3 Purified water To 100 To 100 To 100 To 100 To 100 To 100 Example 1 0.1 - - - - - Example 2 - 0.1 - - - - Example 3 - - 0.1 - - - Reference Example 1 - - - 0.1 - - Reference Example 2 - - - - 0.1 - Vegetable hydrogenated oil 1.50 1.50 1.50 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 0.60 0.60 0.60 Glycerol stearate 1.00 1.00 1.00 1.00 1.00 1.00 Stearyl alcohol 2.00 2.00 2.00 2.00 2.00 2.00 Polyglyceryl-10pentastearate and behenyl alcohol and sodium stearoyl lactylate 1.00 1.00 1.00 1.00 1.00 1.00 Arachidyl behenyl alcohol and arachidyl glucoside 1.00 1.00 1.00 1.00 1.00 1.00 Cetylaryl Alcohol and Cetearyl Glucoside 2.00 2.00 2.00 2.00 2.00 2.00 PEG-100 Stearate & Glycerololate & Propylene Glycol 1.50 1.50 1.50 1.50 1.50 1.50 Caprylic / carboxy triglyceride 11.00 11.00 11.00 11.00 11.00 11.00 Cyclomedicone 6.00 6.00 6.00 6.00 6.00 6.00 Preservative, incense Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Triethanolamine 0.1 0.1 0.1 0.1 0.1 0.1

[Test Example 3] Skin tone improvement effect

To examine the effect of green tea extract of Ginseng Extract on the improvement of skin tone, 60 subjects were divided into 6 groups of 10 persons, and subjects of each group used Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 3 (evening / Day), and the degree of skin tone improvement was evaluated using Facial Stage DM-3 (Moritex, Japan). The improvement rate of the skin tone was determined by the brightness and the color change value of the skin, and the result was shown in Table 4 below. The larger the value of brightness and color change, the better the skin tone.

Test substance Skin tone improvement rate (%) Brightness (mean ± standard deviation) Color (mean ± standard deviation) Formulation Example 1 12 ± 2.34 10 ± 2.92 Formulation Example 2 12 ± 2.51 11 ± 2.09 Formulation Example 3 13 ± 2.25 12 ± 2.47 Comparative Formulation Example 1 10 ± 2.43 9 ± 2.62 Comparative Formulation Example 2 9 ± 2.70 8 ± 2.38 Comparative Formulation Example 3 5 ± 2.34 5 ± 2.05

The results of Table 4 show that Comparative Formulation Example 3 containing no ginseng extract and green tea extract showed no significant skin tone improvement effect while Comparative Examples 1 and 2 containing each of green tea extract of ginseng extract , Skin tone was improved after use compared to before use. In Forms 1 to 3 containing both ginseng extract and green tea extract, remarkably higher skin tone improvement effect than Comparative Formulation Examples 1 and 2 .

Hereinafter, a formulation example of the composition according to the present invention will be described. However, the pharmaceutical composition and the cosmetic composition can be applied to various formulations, which are not intended to limit the present invention but merely to illustrate the present invention.

[Formulation Example 1] Soft capsule

A soft capsule was prepared by mixing 150 mg of the ginseng extract of Reference Example 1, 150 mg of the green tea extract of Reference Example 2, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow radish and 6 mg of lecithin and filling each 400 mg per capsule according to a conventional method.

[Formulation Example 2] Tablets

150 mg of the ginseng extract of Reference Example 1, 150 mg of the green tea extract of Reference Example 2, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate were mixed and 40 mg of 30% ethanol was added to form granules. And the tablet was tableted using a tablet machine.

[Formulation Example 3] Granules

150 mg of the ginseng extract of Reference Example 1, 150 mg of the green tea extract of Reference Example 2, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch were mixed and 100 mg of 30% ethanol was added to form granules, followed by drying at 60 ° C to form granules And filled in the next bubble. The final weight of the contents was 1 g.

[Formulation Example 4] Softening lotion (skin lotion)

The softening longevity was prepared by a conventional method according to the composition shown in Table 5 below.

Compounding ingredient Content (% by weight) The ginseng extract of Reference Example 1 0.1 The green tea extract of Reference Example 2 0.1 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 5] Nutritional lotion (milk lotion)

Nutrition lotion was prepared according to a conventional method according to the composition shown in Table 6 below.

Compounding ingredient Content (% by weight) The ginseng extract of Reference Example 1 0.5 The green tea extract of Reference Example 2 0.5 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capric triglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Liquid paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 6] Nourishing cream

Nutritive creams were prepared according to the compositions listed in Table 7 below in a conventional manner.

Compounding ingredient Content (% by weight) The ginseng extract of Reference Example 1 1.0 The green tea extract of Reference Example 2 1.0 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 7] Massage cream

Massage creams were prepared in a conventional manner according to the composition shown in Table 8 below.

Compounding ingredient Content (% by weight) The ginseng extract of Reference Example 1 1.5 The green tea extract of Reference Example 2 1.5 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 8] Pack

Packs were prepared in a conventional manner according to the composition shown in Table 9 below.

Compounding ingredient Content (% by weight) The ginseng extract of Reference Example 1 0.1 The green tea extract of Reference Example 2 0.1 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 Good Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 9] ointment

Ointments were prepared in a conventional manner according to the composition shown in Table 10 below.

Compounding ingredient Content (% by weight) The ginseng extract of Reference Example 1 0.1 The green tea extract of Reference Example 2 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservative, coloring, fragrance Suitable amount Purified water Balance

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (8)

A composition for external skin application for skin whitening comprising ginseng extract and green tea extract as active ingredients. The composition for external application for skin according to claim 1, wherein the ginseng extract and the green tea extract are contained in an amount of 0.001 to 10 wt% based on the total weight of the composition. The composition for external application for skin according to claim 1, wherein the ginseng extract and green tea extract are mixed at a ratio of 0.1 to 10: 1 by weight. The method according to claim 1, wherein the ginseng extract and green tea extract are water; At least one organic solvent selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform; And a mixed solvent of the organic solvent and water; At a temperature of 10 to 80 DEG C for 3 to 24 hours. A pharmaceutical composition comprising ginseng extract and green tea extract as an active ingredient. [Claim 6] The pharmaceutical composition according to claim 5, wherein the ginseng extract and green tea extract are contained in an amount of 0.001 to 10 wt% based on the total weight of the composition. [Claim 6] The pharmaceutical composition according to claim 5, wherein the ginseng extract and green tea extract are mixed at a weight ratio of 0.1 to 10: 1. 6. The method of claim 5, wherein the ginseng extract and green tea extract are water; At least one organic solvent selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform; And a mixed solvent of the organic solvent and water; , At a temperature of 10 to 80 DEG C for 3 to 24 hours.