KR102326641B1 - Composition comprising extract of buckwheat sprout for strengthening hair root, activating hair growth or preventing alopecia - Google Patents

Abstract

The present invention relates to a composition for strengthening hair roots, promoting hair growth or preventing hair loss, comprising a buckwheat sprout extract containing a homoorientin compound as an active ingredient. The buckwheat sprout of the present invention is an excellent and safe sprout plant with maximized physiologically active ingredients. The buckwheat sprout and the homo orientin compound isolated therefrom are known as VEGF and HGF genes, which are known as factors related to hair root strengthening, as factors related to hair growth. By increasing the expression of the known KFG and IFG-1 genes, male pattern hair loss induced by DHT (Dihydrotestosterone), stress hair loss induced by Substance P, and hair loss symptoms caused by environmental harmful factors such as fine dust are alleviated, and conventional hair loss Compared to the treatment minoxidil or caffeine, which is a hair root strengthening substance, its effect is superior. For this reason, the buckwheat sprout extract containing homo-orientin of the present invention as an active ingredient can be used in various products such as pharmaceutical compositions, cosmetic compositions, or health functional foods for preventing hair growth or hair loss.

Description

A composition comprising extract of buckwheat sprout for strengthening hair root, activating hair growth or preventing alopecia, containing buckwheat sprout extract.

The present invention relates to a composition for strengthening hair roots, promoting hair growth, or preventing hair loss, containing buckwheat sprout extract.

Human hair is very important because it plays an intrinsic role in social and sexual communication, as well as its primary role in protecting the skin and scalp.

The hair cycle consists of an anagen phase, a catagen phase, and a telogen phase, and the hair is maintained by repeating hair growth and hair loss while repeating the cycle. Hair growth period is 3 to 5 years for men, 4 to 6 years for women, 30 to 45 days for hair regression, and 3 to 4 months for resting period. When the final stage of the telogen phase is reached, the generator naturally begins to generate new hair and the hair is maintained. Since hair loss is a natural phenomenon, alopecia, which is recognized as a hair loss disease, refers to a state in which there are relatively many hairs in a telogen state and there are few hairs.

The cause of hair loss is not yet known precisely, but it is a major factor that affects hair growth. It inhibits or decreases the function of dermal papilla, which is related to the regulation of the hair cycle, abnormal hair cycle due to male hormone action, and scalp. Abnormal changes in the hair cycle due to a decrease in blood flow to the hair follicles, antihypertensive drugs, mental stress, physical stimulation, and environmental pollution are being discussed.

In the past, hair loss mainly occurred in middle-aged and elderly people, but recently, hair and scalp damage due to mousse, perm, dyeing, and dry heat, increase in social and environmental stress, and changes in food due to westernization of diet, etc. Hair loss is remarkably occurring in young people in their 30s, and not only male pattern hair loss but also female pattern hair loss is on the rise.

For this reason, although many therapeutic agents for preventing hair loss are on the market worldwide, there are still few drugs or technologies that can satisfy the therapeutic effect. Currently, it is known that the progress of hair loss can be slowed down to some extent by using the topical 'minoxidil' or the oral drug 'Propecia (finasteroid)' in the early stages of hair loss. There are difficulties, and body hair grows in unwanted areas, sexual dysfunction such as erectile dysfunction and loss of libido, and side effects such as dizziness, headache, edema and skin rash may occur. There is a problem that cannot be.

Among them, 'Minoxidil' is a drug approved by the US FDA, and although the exact treatment mechanism is not yet clear, dermal papilla cells (DPCs) that are reported to affect the hair cycle through the secretion of proteins such as various growth factors. ) is known to have the effect of proliferating the growth of dermal papilla cells (DPCs) by lowering apoptosis. In addition, drugs such as 'Propecia', a 5-alpha reductase inhibitor, inhibit the production of dihydrotestosterone (DHT), a male hormone that causes hair loss, and prevent the progression of male pattern hair loss. It is known that measuring the activity inhibitory effect of 5-alphareductase or the growth and proliferation effect of dermal papilla cells (DPCs) for a certain substance shows how effectively the ingredient prevents hair loss and contributes to hair growth. It can be used as an indicator to know.

Hair is made up of keratin protein and grows from hair follicles in the dermis. Scalp hair follicles are composed of dermal papilla cells, keratinocytes, inner and outer hair root sheath cells, and melanocytes. In addition, hair follicle dermal papilla cells are known to be closely related to hair growth.

Recently, studies on many regulatory factors involved in hair growth and hair loss mechanisms are being actively conducted at various research institutes. continuously reported. Growth factors that affect hair growth include EGF, EGFF, VEGF, EGFR, HGF/SF, c-MET, FGF, FGFR, IGF, IGF-IR, TGF-β and TGF-βR. It is known to affect the hair cycle by regulating its activity. In addition, it is known that the Wnt pathway and Bmp signaling play a decisive role in the proliferation or differentiation of hair follicle stem cells in the bulge region.

On the other hand, recently, the number of hair loss population continues to increase due to environmental stress, etc. along with side effects due to environmental pollution and misuse of drugs. The development of new agents for preventing hair loss is emerging.

In addition, among the currently developed anti-hair loss agents, especially synthetic compound formulations, there is a problem in causing various side effects on the skin.

Looking at these prior arts, Republic of Korea Patent No. 10-0800359 (composition showing hair loss prevention and hair growth promoting effect) discloses the use of natural plants such as silk grass, ginseng, jujube, goji berry and cornus oil, and a Korean registered patent No. 10-0773457 (composition having a hair growth promoting effect and a method of using the same) discloses a composition effective for preventing hair loss and promoting hair growth using ginseng, white sorghum, angelica, astragalus, safflower jasmine, hyssop, and headworm as main raw materials.

As such, there are many developments as a mixed extract using various kinds of herbs to promote hair growth, but the discovery of substances having the effects of hair growth, hair root strengthening, and hair loss prevention from a single component of buckwheat sprout extract is still pending. Research is lacking.

Buckwheat ( Fagopyrum esculentum ) is widely distributed around the world, including Australia. In Korea, it is cultivated all over the country and is widely used as a raw material for food. In particular, buckwheat buds grown as bean sprouts contain more Rutin than buckwheat seeds, which is effective in preventing and treating various vascular diseases. In addition, aspartic acid, glutamic acid, and lysine are 30% higher in sprouts than seeds, and the content of minerals, fiber, calcium, and magnesium is high, so it is a high-alkaline food. Buckwheat sprouts have a shape similar to that of bean sprouts and bean sprouts, and their length (30 mm to 50 mm) is smaller and curled like a flower bud, so the shape is unique and has a pale yellow color. Also, in the case of bean sprouts and bean sprouts, it is difficult to eat them raw because of their unique odor and requires cooking, whereas buckwheat sprouts have only their own flavor and no other odors, so it is an important feature that they can be eaten raw.

Regarding the manufacture of a cosmetic composition for whitening and antioxidant in which rutin glycosides are converted to quercetin by treating the bitter buckwheat sprout extract with a sugar-binding enzyme in relation to buckwheat sprouts, Republic of Korea Patent No. 10-1201916 (Bitter buckwheat by enzyme reaction) A method for producing a sprout extract and a cosmetic composition for whitening and antioxidant containing the extract as an active ingredient) have been disclosed, and comprising buckwheat sprouts grown by treatment with jasmonic acid or methyl jasmonate as an active ingredient Regarding a pharmaceutical composition for improving obesity or reducing body fat, Korean Patent Registration No. 10-1282708 (Anti-obesity or body fat reduction composition containing MJA-treated buckwheat sprouts) has been disclosed.

On the other hand, the present inventors studied the various physiological activities of the buckwheat sprout extract, and the buckwheat sprout extract has the effect of strengthening hair roots, promoting hair growth or preventing hair loss, so a pharmaceutical composition for preventing hair growth or hair loss, cosmetic composition or health The present invention was completed by confirming that it can be used as a functional food.

Republic of Korea Patent Registration No. 10-0800359 (Title of the invention: composition showing hair loss prevention and hair growth promoting effect) Republic of Korea Patent Registration No. 10-0773457 (composition having the effect of promoting hair growth and method of using the same) Republic of Korea Patent No. 10-1201916 (Method for producing bitter buckwheat sprout extract by enzyme reaction and cosmetic composition for whitening and antioxidant containing the extract as an active ingredient) Republic of Korea Patent No. 10-1282708 (Anti-obesity or body fat reduction composition containing MJA-treated buckwheat sprouts)

It is an object of the present invention to provide a composition for strengthening hair roots, promoting hair growth or preventing hair loss, containing a buckwheat sprout extract containing a homoorientin compound as an active ingredient. The buckwheat sprout extract or homo orientin compound is characterized in that it promotes the expression of growth factors in dermal papilla cells (DPCs) or hair cells, and the growth factors include hepatocyte growth factor (HGF), keratinocyte growth factor At least one selected from (Keratinocyte growth factor; KGF), insulin-like growth factor (IGF), or vascular endothelial growth factor (VEGF) may be included.

The present invention relates to a cosmetic composition for strengthening hair roots, promoting hair growth, or preventing hair loss, comprising a buckwheat sprout extract comprising Homoorientin of the following formula (1) as an active ingredient.

[Formula 1]

The buckwheat sprout extract may be extracted from buckwheat sprout using an aqueous solution of C1-C4 alcohol as a solvent.

In another aspect, the present invention relates to a cosmetic composition for strengthening hair roots, promoting hair growth or preventing hair loss, containing homo orientin of Formula 1 above.

The buckwheat sprout extract or homo orientin increases HGF (Hepatocyte growth factor) or VEGF (Vascullar Endotherial Growth Factor) gene expression in dermal papilla cells, or KGF (Keratinocyte growth factor), IGF-1 (Insulin-like) in mother cells. It is characterized by increasing gene expression of growth factor 1).

In addition, the buckwheat sprout extract or homoorientin (Homoorientin) inhibits the gene expression of androgen receptor or TGF-β2 (Transforming growth factor beta 2) in dermal papilla cells, characterized in that it has an inhibitory effect on male pattern hair loss. .

In addition, the buckwheat sprout extract or homo orientin has the effect of inhibiting stress-related hair loss by inhibiting gene expression of DKK-1 (Dickkopf-related protein 1) or IL-6 (Interleukin-6) in hair cells, or, It is characterized in that it inhibits the gene expression of TGF-β2 (Transforming growth factor beta 2) and IL-6 (Interleukin-6) in hair cells to inhibit hair loss caused by fine dust, an environmental harmful factor.

In addition, the present invention provides a health functional food for strengthening hair roots, promoting hair growth or preventing hair loss, containing a buckwheat sprout extract containing homo orientin as an active ingredient.

In another aspect, the present invention relates to a pharmaceutical composition for strengthening hair roots, promoting hair growth or preventing hair loss, containing a buckwheat sprout extract containing homo orientin as an active ingredient. Accordingly, the present invention also relates to a pharmaceutical composition for strengthening hair roots, promoting hair growth, or preventing hair loss containing homo orientin.

Hereinafter, the present invention will be described in detail.

The buckwheat sprout extract may be extracted from buckwheat sprouts using an aqueous solution of C1-C4 alcohol as a solvent, and the C1-C4 alcohol may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol. C1-C4 alcohol in the aqueous solution is contained in 50-80% (w/w).

The aqueous solution of C1-C4 alcohol used in the preparation of the buckwheat sprout extract may be used in an amount of 1 to 40 times the weight based on the weight of buckwheat sprout, preferably 5 to 40 times the weight. Extraction conditions of the buckwheat sprout extract may be 0.5 to 48 hours at 20 ~ 100 ℃. The above process can be repeated 1 to 4 times.

The buckwheat sprouts may be sprouts 5 to 9 days after germination from buckwheat seeds, and preferably sprouts 7 to 8 days after germination from buckwheat seeds.

The buckwheat sprout extract can be prepared as a fraction by dissolving the extract in water as a conventional method in the art and then additionally fractionating it using one or more solvents selected from the group consisting of n-hexane, chloroform and ethyl acetate.

The buckwheat sprout extract of the present invention may contain about 0.5 to 3% by weight of homo-orientin, and the fraction of buckwheat sprout may contain about 10-30% by weight of homo-orientin.

The preparation of the fraction is a more detailed method, and after extracting and concentrating buckwheat sprouts with water, C1-C4 alcohol or a mixed solvent thereof, water is added to the buckwheat sprouts, and then suspended, preferably 1 by weight of the buckwheat sprout extract -1000 times, more preferably 1~500 times, most preferably 1~50 times by adding water to the suspension, and then adding a solvent selected from hexane, chloroform or ethyl acetate to the suspension to obtain a buckwheat sprout fraction. can be manufactured. Other fractionation conditions are not limited, but after preparing a suspension by adding 1 to 50 times the weight of the buckwheat sprout extract to the buckwheat sprout extract, a solvent of hexane, chloroform or ethyl acetate in the same amount as the water is added. can be added and fractionated. In addition, these solvent fractions may be fractionated by single selection after crude extraction, and may be fractionated sequentially by selecting the order.

The preparation temperature of the extract or a fraction thereof may be 20 to 100 ℃, but is not limited thereto. Extraction or fractionation time is not particularly limited, but it is preferable to extract within 10 minutes to 2 days, and as an extraction device, a conventional extraction device, an ultrasonic crushing extractor or a fractionator may be used. The buckwheat sprout extract or fraction thus prepared may be dried with hot air, dried under reduced pressure or freeze-dried to remove the solvent. In addition, the buckwheat sprout extract or fraction may be purified and used using column chromatography.

The chromatography is silica gel column chromatography (silica gel column chromatography), LH-20 column chromatography (LH-20 column chromatography), ion exchange resin chromatography (ion exchange resin chromatography), medium pressure liquid chromatography (medium pressure liquid chromatography) chromatography), thin layer chromatography (TLC), silica gel vacuum liquid chromatography, and high performance liquid chromatography may be selected.

In addition, the present invention provides a pharmaceutical composition for strengthening hair roots, promoting hair growth or preventing hair loss, containing buckwheat sprout extract, fraction or homo orientin isolated therefrom. The buckwheat sprout extract, fraction or homo orientin separated therefrom may be added to the pharmaceutical composition of the present invention in an amount of 0.0001 to 30% by weight.

The pharmaceutical composition may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc., oral dosage forms, external preparations, suppositories, and sterile injection solutions according to conventional methods, respectively. Carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, it is prepared using diluents or excipients, such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and such solid preparations include at least one excipient in the extract of the present invention, for example, starch, calcium carbonate, sucrose or lactose, It is prepared by mixing gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid formulations for oral use include suspensions, solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients, for example, wetting agents, sweeteners, fragrances, preservatives, etc. may be included. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, and the like can be used.

The dosage of the pharmaceutical composition of the present invention will vary depending on the age, sex, and weight of the subject to be treated, the specific disease or pathological condition to be treated, the severity of the disease or pathological condition, the route of administration, and the judgment of the prescriber. Dosage determination based on these factors is within the level of the skilled artisan, and dosages generally range from 0.01 mg/kg/day to approximately 2000 mg/kg/day. A more preferred dosage is 1 mg/kg/day to 500 mg/kg/day. Administration may be administered once a day, or may be administered in several divided doses. The above dosage does not limit the scope of the present invention in any way.

The pharmaceutical composition of the present invention may be administered to mammals such as mice, livestock, and humans by various routes. Any mode of administration can be envisaged, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebrovascular injection. Since the extract of the present invention has almost no toxicity and side effects, it is a drug that can be safely used even when taken for a long period of time for the purpose of prevention.

The cosmetic composition of the present invention is not particularly limited, and if it is a cosmetic composition for hair, for example, shampoo, conditioner, treatment, hair oil, emulsion, scrape, mousse, hair lotion, hair gel, hair pack, hair ampoule, soap and the like, and the dosage form is not particularly limited, and in more detail, hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream , Hair Moisture Cream, Hair Massage Cream, Hair Wax, Hair Aerosol, Hair Pack, Hair Nutrition Pack, Hair Soap, Hair Cleansing Foam, Hair Oil, Hair Drying Agent, Hair Preservative, Hair Dyeing Agent, Hair Wave Agent, Hair Decoloring Agent, Hair Gel, It may be selected from a hair glaze, a hair dresser, a hair lacquer, a hair moisturizer, a hair mousse, or a hair spray.

The present invention may also provide a cosmetic composition containing a buckwheat sprout extract, a fraction thereof, or a homo orien. In particular, the cosmetic composition may have an excellent skin moisturizing function. The cosmetic composition may include all commonly used ingredients. For example, it may include general auxiliary ingredients such as emulsifiers, thickeners, emulsifiers, surfactants, lubricants, alcohols, water-soluble polymers, gelling agents, stabilizers, vitamins, inorganic salts, emulsifiers, and fragrances. More specifically, when the formulation of the cosmetic composition of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica as a carrier component , talc or zinc oxide may be used. When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydro It may contain a propellant such as carbon, propane-butane or dimethyl ether. When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene fatty acid esters of glycol, 1,3-butylglycol oil, glycerol fatty esters, polyethylene glycol or sorbitan. When the formulation of the cosmetic composition of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracanth may be used. When the formulation of the cosmetic composition of the present invention is surfactant-containing cleansing, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, acetionate, imidazolinium derivative, methyl taurate, sarcosinate as carrier components. , fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol fatty acid ester, etc. may be used. The cosmetic composition of the present invention may further contain excipients including fluorescent substances, fungicides, hydrophobicity-inducing substances, moisturizers, fragrances, fragrance carriers, proteins, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, and the like. . According to the formulation or purpose of use, the amount of the ingredients may be selected within a range that does not impair the intrinsic effect of the cosmetic composition. The amount of the components added may be, for example, 0.1 to 10% by weight, preferably 0.1 to 6% by weight, based on the total weight of the composition, but is not limited thereto.

In addition, the present invention provides a health functional food for strengthening hair roots, promoting hair growth, or preventing hair loss, comprising a nutritively acceptable food supplement additive together with a buckwheat sprout extract, a fraction thereof, or homo orientin. The buckwheat sprout extract may be added to the health functional food of the present invention in an amount of 0.001 to 100% by weight. The health functional food of the present invention includes the form of tablets, capsules, pills, or liquids, and the food to which the extract of the present invention can be added includes, for example, various drinks, meat, sausage, bread, candy, Snacks, noodles, ice cream, dairy products, soups, ionized beverages, beverages, alcoholic beverages, gum, tea, and vitamin complexes.

The present invention relates to a composition for strengthening hair roots, promoting hair growth or preventing hair loss, comprising a buckwheat sprout extract containing a homo orientin compound as an active ingredient. The buckwheat sprout of the present invention is an excellent and safe sprout plant with maximized physiologically active ingredients. The buckwheat sprout and the homo orientin compound isolated therefrom are known as VEGF and HGF genes, which are known as factors related to hair root strengthening, as factors related to hair growth. By increasing the expression of the known KFG and IFG-1 genes, male pattern hair loss induced by DHT (Dihydrotestosterone), stress hair loss induced by Substance P, and hair loss symptoms caused by environmental harmful factors such as fine dust are alleviated, and conventional hair loss Compared to the treatment minoxidil or caffeine, which is a hair root strengthening substance, its effect is superior.

For this reason, the buckwheat sprout extract containing homo-orientin of the present invention as an active ingredient can be used in various products such as pharmaceutical compositions, cosmetic compositions, or health functional foods for preventing hair growth or hair loss.

1 shows the HGF mRNA expression increasing activity of 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts and homoorientin.
Figure 2 shows the VEGF mRNA expression increasing activity of 70% (w / w) ethanol aqueous solution extract of buckwheat sprouts and homo orientin.
Figure 3 shows the KGF mRNA expression increase activity of 70% (w / w) ethanol aqueous solution extract of buckwheat sprouts and homo orientin.
Figure 4 shows the activity of increasing the expression of IGF-1 mRNA of 70% (w / w) ethanol aqueous solution extract of buckwheat sprouts and homo orientin.
Figure 5 shows the 70% (w / w) ethanol aqueous solution extract of buckwheat sprouts and the expression inhibitory activity of homo orientin in the male pattern hair loss model.
Figure 6 shows the expression inhibitory activity of TGF-β2 mRNA of 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts and homoorientin in male pattern hair loss model.
Figure 7 shows the expression inhibitory activity of DKK-1 mRNA of 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts and homo orientin in a stress hair loss model.
Figure 8 shows the expression inhibitory activity of TGF-β2 mRNA in a 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts and homo orientin in a stress hair loss model in a stress hair loss model.
Figure 9 shows the IL-1α mRNA expression inhibitory activity of 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts and homo orientin in a stress hair loss model.
10 shows the expression inhibitory activity of DKK-1 mRNA of 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts and homo orientin in the fine dust-induced hair loss model.
11 shows the IL-6 mRNA expression inhibitory activity of 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts and homo orientin in the fine dust-induced hair loss model.
12 is an HPLC spectrum result showing the active ingredient peak in 70% (w/w) ethanol extract of buckwheat seeds before germination. The E peak in the graph represents Rutin.
13 is an HPLC spectrum result showing active ingredient peaks in 70% (w/w) ethanol extract when buckwheat seeds germinate and sprout on the 7th to 8th days. In the graph, the A peak is Homoorientin, the B peak is Orientin, the C peak is Unknown (unidentified compound), the D peak is Vitexin, the E peak is Rutin, F The peak represents Isovitexin.
Figure 14 shows the HGF mRNA expression increasing activity of 70% (w / w) ethanol aqueous solution extract and homoorientin of each sprout.
15 shows the VEGF mRNA expression increasing activity of 70% (w/w) ethanol aqueous solution extract and homo orientin of each sprout.
16 shows the results ^{of 1} H NMR and ¹³ C NRM of the homoorientin compound.

Hereinafter, preferred embodiments of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, it is provided so that this disclosure will be thorough and complete, and will fully convey the spirit of the invention to those skilled in the art.

<Preparation Example 1. Preparation of 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts>

The washed buckwheat seeds (bitter buckwheat) germinated for 7-8 days to obtain buckwheat sprouts, sterilized and dried to prevent microbial contamination, and dried so that the moisture content was 15% by weight or less (each extract thereafter) When manufacturing, buckwheat sprouts refer to dried and sterilized products).

After adding 6 kg of 70 (w/w)% ethanol to 0.6 kg of dried buckwheat sprouts, extraction was repeated three times for 2 days at 60°C, and the filtrate obtained by filtration was concentrated under reduced pressure using a vacuum concentrator to prepare 0.09 kg of buckwheat sprout extract. .

<Preparation Example 2. Preparation of ethyl acetate fraction of buckwheat sprouts>

50 g of the buckwheat sprout extract prepared in Preparation Example 1 was suspended in 500 g purified water, the same amount of ethyl acetate was added, extracted three times, fractionated in a separatory funnel, the ethyl acetate layer was recovered, and then concentrated under reduced pressure to produce buckwheat sprouts. 12.5 g of an ethyl acetate fraction were obtained.

<Preparation Example 3. Extract of 50% (w/w) ethanol aqueous solution of buckwheat sprouts>

A buckwheat sprout extract was prepared under the same conditions as in Preparation Example 1, but a 50% (w/w) aqueous ethanol solution was used as an extraction solvent.

<Preparation Example 4. Extract of 80% (w/w) ethanol aqueous solution of buckwheat sprouts>

A buckwheat sprout extract was prepared under the same conditions as in Preparation Example 1, but an 80% (w/w) aqueous ethanol solution was used as an extraction solvent.

<Preparation Example 5. Homoorientin purified product>

The extract of Preparation Example 1 was purified by HPLC to obtain a fraction showing the same peak as each standard compound. In particular, for homo orientin, the fraction was obtained and distilled under reduced pressure to obtain a compound having a purity of 99% or more (HPLC conditions) is the condition described in Example 1).

Sigma-Aldrich products were used as a standard for confirming the content of homo-orientin. Thereafter, homo orientin compounds isolated from buckwheat sprouts were used in each experiment. The NMR analysis result of the homo-orientin compound separated from the peak at the same position as the standard was confirmed to be consistent with the existing paper, which is also shown in FIG. 16 .

<Comparative Preparation Example 1. Water Extract of Buckwheat Sprout>

After adding 6 kg of water to 0.6 kg of dried buckwheat sprouts, extraction was repeated three times for 2 days at 60°C, and the filtered filtrate was concentrated with a vacuum concentrator to obtain a water extract of buckwheat sprouts.

<Comparative Preparation Example 2. 1,3-butylene glycol extract of buckwheat sprout>

After adding 6 kg of 1,3-butylene glycol to 0.6 kg of dried buckwheat sprouts, extraction was repeated three times for 2 days at 40°C, and the filtered filtrate was freeze-dried to obtain a water extract of buckwheat sprouts.

<Comparative Preparation Example 3. Extract of 30% (w/w) ethanol aqueous solution>

A buckwheat sprout extract was prepared under the same conditions as in Preparation Example 1, but a 30% (w/w) aqueous ethanol solution was used as an extraction solvent.

<Comparative Preparation Example 4. Ethanol Extract>

A buckwheat sprout extract was prepared under the same conditions as in Preparation Example 1, but ethanol (purity of 99% or more) was used as an extraction solvent.

<Comparative Preparation Example 5. Water layer fraction of buckwheat sprouts>

The water layer remaining after ethyl acetate fractionation in Preparation Example 2 was freeze-dried to obtain a water layer fraction.

<Comparative Preparation Example 6. 70% (w/w) ethanol aqueous solution extract of buckwheat seeds>

An extract was prepared as in Preparation Example 1, but the extraction target was a dry general buckwheat seed.

<Comparative Preparation Example 7. Buckwheat Seed Water Extract>

An extract was prepared as in Comparative Preparation Example 1, but the extraction target was dried general buckwheat seeds.

<Comparative Preparation Example 8. Ethanol Extract of Buckwheat Seed>

An extract was prepared as in Comparative Preparation Example 4, but the extraction target was dried general buckwheat seeds.

<Comparative Preparation Example 9. Preparation of 70% (w/w) ethanol aqueous solution extract of germinated buckwheat>

An extract was prepared as in Preparation Example 1, but the extraction target was buckwheat seeds 1 day after germination.

<Comparative Preparation Example 10. Preparation of water extract of germinated buckwheat>

An extract was prepared as in Comparative Preparation Example 1, but the extraction target was buckwheat seeds 1 day after germination.

<Comparative Preparation Example 11. Preparation of Ethanol Extract of Germinated Buckwheat>

An extract was prepared as in Comparative Preparation Example 4, but the extraction target was buckwheat seeds 1 day after germination.

<Comparative Preparation Example 12. 70% (w/w) ethanol aqueous solution extract of sprout wheat>

An extract was prepared as in Preparation Example 1, but the extraction target was wheat sprout grown for 7-8 days.

<Comparative Preparation Example 13. 70% (w/w) ethanol aqueous solution extract of germinated brown rice>

An extract was prepared as in Preparation Example 1, but the extraction target was brown rice 1 day after germination.

<Comparative Preparation Example 14. 70% (w/w) ethanol aqueous solution extract of sprouted oats>

An extract was prepared as in Preparation Example 1, but the extraction target was sprouted oats grown for 7-8 days.

<Comparative Preparation Example 15. 70% (w/w) ethanol aqueous solution extract of sprouted barley>

An extract was prepared as in Preparation Example 1, but the extraction target was sprouted barley grown for 7-8 days.

<Experimental Example 1. Analysis of the content of homo-orientin compounds in buckwheat sprout extracts and fractions>

The content analysis of the buckwheat sprout extract of Preparation Example 1, the buckwheat sprout fraction of Preparation Example 2, and the purified homo orientin compound was analyzed for purity using high performance liquid chromatography (HPLC) under the conditions described in Table 1 below, The results are shown in Table 2 below.

In addition, this HPLC result was again shown in Figs. 12 and 13 by confirming the peaks for each compound in the state of buckwheat seeds and 7 to 8-day-old buckwheat sprouts for the extract extracted with 70% (w/w) aqueous ethanol solution.

Column Capsulepack C18 4.6 x 250 mm Shiseido Column temperature 30℃ Flowrate 1.0 mL/min Detector UV 350nm Injection volume 20 μl Mobile phase 15% Acetonitrile

test substance Homoorientin content (%) Orientin content (%) Rutin content (%) Preparation Example 1 1.21% 0.68 0.60 Preparation 2 24.1% 13.7 12.2 Comparative Preparation Example 1 0.24% 0.14 0.12

As a result of the analysis, the buckwheat sprout extract prepared using a 70% (w/w) aqueous ethanol solution as a solvent and its fractions had a very high content of homo-orientin, especially compared to the 70% (w/w) ethanol aqueous solution extract of seeds. It was confirmed that a large amount of active ingredients were produced in the sprouting state of 7-8 days. However, it can be seen that the extract prepared using water as a solvent contains very little of these components.

<Experimental Example 2. Cell culture, synthesis of primers>

DPCs (Human Dermal Papilla cells: dermal papilla cells) and HHGMC (Human Hair Germinal Matrix Cells: hair cells) cells were used, and the DPCs cell line was 10% Fetal Bovine Serum (FBS), 1% antibiotics (Anti). DMEM (Dulbecco Modified Eagle Medium) medium containing P), using MSCM (Mesenchymal Stem Cell Medium) medium containing 1% mesenchymal stem cell growth supplement (MSCGS), 37 ℃, 5% CO ₂ Conditions were cultured.

In addition, the primer sequence to be used in the gene amplification experiment in the present invention was synthesized by Cosmogene Tech (Korea) as follows.

Primer Sense Antisense HGF 5'-ATG TCA GCC CTG GAG TTC CAT GAT-3' 5'-AGC GTA CCT CTG GAT TGC TTG TGA-3' VEGF 5'-TGC AGA TTA TGC GGA TCA AAC C-3' 5'-TGC ATT CAC ATT TGT TGT GCT GTA G-3' KGF 5'-AAG GGA CCC AGG AGA TGA AGA-3' 5'-TGC CAC AAT TCC AAC TGC C-3' IGF-1 5'-AGC CTG TCC ACC CTT GAG AA-3' 5'-CCC TGG AGC CAC AGA GCA T-3' Androgen receptor 5'-CCT GGC TTC CGC AAC TTA CAC-3' 5'-GGA CTT GTG CAT GCG GTA CTC A-3' TGF-β2 5'-CAC CAT AAA GAC AGG AAC CTG-3' 5'-GGA GGT GCC ATC AAT ACC TGC-3' DKK-1 5'-CAT CAG ACT GTG CCT CAG GA-3' 5'-CCA CAG TAA CAA CGC TGG A-3' IL-6 5'-CCA GGA GCC CAG CTA TGA AC-3' 5'-CCC AGG GAG AAG GCA ACT G-3' β-actin 5'-GGC ACC CAG CAC AAT GAA G-3' 5'-CCG ATC CAC ACG GAG TAC TTG-3'

<Experimental Example 3. Conditions for RNA extraction, cDNA synthesis and real time PCR>

DMEM medium containing bovine serum and dermal papilla cells (DPCs, Cell Bio) or MSCM medium containing 5% bovine serum and hair cells (Human Hair Germinal Matrix Cell, HHGMC, ScienCell) were placed in a 6 well multi plate (corning), respectively. After culturing for 24 hours, cells for experiments were prepared by replacing each well with a serum-free medium.

The non-irradiated group was treated with purified water used as a solvent for each raw material sample.

Each hair loss cell model for the experimental group was prepared as follows.

For male pattern hair loss-inducing cells, 5α-Dihydrotestosterone (DHT) solution was treated in dermal papilla cells, and the sample prepared in the present invention was added and cultured for 48 hours. For hair loss due to stress, the hair cells were treated with Substance P, Acetate salt hydrate (Sigma aldrich, S6883) secreted when stress occurred, and the sample prepared in the present invention was added and cultured for 24 hours. For fine dust-induced hair loss conditions, particular matter 10 (PM10-like, PAH's, ERMCZ100, Sigma-Aldrich, Germany), which is fine dust, and the sample prepared in the present invention were added to hair cells, and the sample was treated and then cultured for 24 hours. .

As a positive control group of the samples of the present invention, minoxidil or caffeine, known as a substance that induces hair root strengthening and hair growth, and inhibits hair loss, was used.

After each treatment time, each cell treated with each sample, a positive control material, and a hair loss inducer was washed with PBS (Phosphate buffered saline), and then the cultured cells were treated with QIAzol ^® (QIAGENL ^® ,USA) for cell lysis After proceeding, RNA is isolated using the protocol provided by the manufacturer (QIAGENL ^{® ).} After quantifying the isolated RNA using Qubit ^® fluoremeter with RNABRAssaykit, cDNA was synthesized and real-time PCR was performed. For cDNA synthesis, a cDNA synthesis kit (qPCRBIO cDNA Synthesis Kit, PCRBIOSYSTEMS, London, UK) was used, and the experiment was performed according to the kit method. Real-time PCR amplified the gene using Real-time PCR Kit (2x qPCRBIO SyGreen Blue mix Lo-ROX, PCRBIOSYSTEMS, London, UK) and then quantitatively analyzed the amplified product. Real-time PCR conditions are 5 seconds at 95°C, 25 seconds at 60°C, and 40 cycles of each primer.

<Experimental Example 4. Investigation of expression levels of HGF and VEGF related genes for hair root strengthening of 70% (w/w) ethanol aqueous solution extract and indicator homo orientin of buckwheat sprouts>

To evaluate the efficacy of hair root strengthening, HGF (Hepatocyte growth factor) and VEGF (Vascullar Endotherial Growth Factor) gene expression experiments were performed using dermal papilla cells (DPCs, Cell Bio). Minoxidil was used as a positive control. The mRNA expression levels of each gene are shown in Table 4 and FIG. 1 , and Table 5 and FIG. 2 .

test group experimental concentration
(ppm) HGF mRNA expression rate (%) Stdev. Non-irradiated group (Control) 0 100.00 0.00 Minoxidil One 114.74 9.20 Preparation Example 1:
70% (w/w) ethanol aqueous solution extract of buckwheat sprouts 100 126.40 6.98 Preparation Example 5:
Homoorientin 10 121.81 1.37

test group experimental concentration
(ppm) VEGF mRNA expression rate (%) Stdev. Non-irradiated group (Control) 0 100.00 0.00 Minoxidil One 114.97 4.27 Preparation Example 1:
70% (w/w) ethanol aqueous solution extract of buckwheat sprouts 100 171.68 1.38 Preparation Example 5:
Homoorientin 10 134.14 1.61

Compared to Control, 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts induces the formation of blood vessels in hair follicles and increases HGF gene expression expressed in the early growth stage of DPCs by 26.4% It was confirmed that it has the effect of strengthening the hair root by increasing the VEGF gene expression that induces cell proliferation and migration by 71.68% by increasing the supply of nutrients during the growth phase of the hair through the formation of blood vessels around the hair follicle. . In addition, homo-orientin used as an indicator also increased HGF gene expression by 21.8% and VEGF gene expression by 34.2% at a concentration 10 times lower than the extract, so that both the 70% (w/w) ethanol aqueous solution extract of buckwheat sprout and the indicator It has the effect of strengthening the hair root by increasing gene expression.

<Experimental Example 5. Investigation of expression levels of KGF and IGF-1 hair growth-related genes of 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts and indicator homo orientin>

To evaluate hair growth efficacy, gene expression experiments of KGF (Keratinocyte growth factor, FGF-7) and IGF-1 (Insulin-like growth factor 1) were performed through real time PCR. As experimental cells, mother cells were used, and as a positive control, Caffeine was used. Each mRNA expression level is shown in Table 6 and Figure 3, Table 7 and Figure 4.

test group experimental concentration
(ppm) KGF mRNA expression rate (%) Stdev. Non-irradiated group (Control) 0 100.00 0.00 Caffeine One 119.63 6.79 Preparation Example 1:
70% (w/w) ethanol aqueous solution extract of buckwheat sprouts One 124.27 4.76 Preparation Example 5:
Homoorientin 10 120.30 2.40

test group experimental concentration
(ppm) IGF-1 mRNA expression rate (%) Stdev. Non-irradiated group (Control) 0 100.00 0.00 Caffeine One 131.20 5.67 Preparation Example 1:
70% (w/w) of buckwheat sprouts
Ethanol aqueous solution extract One 133.66 3.06 Preparation Example 5:
Homoorientin 10 113.63 4.51

Compared to Control, the 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts increased the expression of KGF gene involved in cell proliferation and division in hair follicles by 24.3%, and the gene of IGF-1, a hair growth promoting factor. By increasing the expression by 33.7%, it was confirmed that there was an effect on hair growth, and homoorientin, an index, also increased the expression of KGF gene by 20.3% and the expression of IGF-1 gene by 13.6% at a concentration 10 times lower than the extract. It was confirmed that both the 70% (w/w) ethanol aqueous solution extract and indicator of buckwheat sprouts had the effect of promoting hair growth.

<Experimental Example 6. Investigation of androgen receptor, TGF-β2 expression levels of male pattern hair loss-inducing genes of 70% (w/w) ethanol aqueous solution extract and indicator homoorientin of buckwheat sprouts>

To evaluate the male-pattern hair loss mitigation effect, gene expression experiments of AR (Androgen receptor) and TGF-β2 (Transforming growth factor beta 2) were performed through real time PCR. As experimental cells, dermal papilla cells (DPCs, Cell Bio) were used, and male pattern hair loss was induced with 5α-Dihydrotestosterone (DHT) solution. Minoxidil was used as a positive control. Each mRNA expression level is shown in Table 8 and Figure 5, Table 9 and Figure 5.

test group AR mRNA expression rate (%) Stdev. Non-irradiated group (Control) 67.25 8.97 DHT (50 nM) 100.00 0.00 DHT (50 nM) + Minoxidil (1 ppm) 59.28 8.08 DHT (50 nM) +
Preparation Example 1: 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts
(100 ppm) 54.67 5.67 DHT (50 nM) + Preparation Example 5: Homoorientin (10 ppm) 67.69 4.25

test group TGF-β2 mRNA expression rate (%) Stdev. Non-irradiated group (Control) 28.05 5.91 DHT (50 nM) 100.00 0.00 DHT (50 nM) + Minoxidil (1 ppm) 70.75 4.93 DHT (50 nM) +
Preparation Example 1: 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts
(100 ppm) 42.54 8.01 DHT (50 nM) + Preparation Example 5: Homoorientin (10 ppm) 34.68 3.42

In order to induce male pattern hair loss, the 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts inhibited the expression of Androgen receptor, a DHT transporter, by 45.3% compared to the condition treated with DHT, and the expression of TGF-β2, which is involved in hair regression, was inhibited. It has the effect of alleviating male pattern hair loss by inhibiting 57.5%, and homoorientin, an index, inhibited AR expression by 32.3% at a concentration 10 times lower than the extract, and inhibited TGF-β2 gene expression by 65.3%, thereby inhibiting 70% of buckwheat sprouts ( w/w) It was confirmed that both the ethanol aqueous solution extract and the index had the effect of alleviating male pattern hair loss.

<Experimental Example 7. Investigation of DKK-1 and IL-6 expression levels of buckwheat sprout 70% (w/w) ethanol aqueous solution extract and indicator homoorientin stress-induced hair loss>

To evaluate the stress-related hair loss mitigation effect, hair cells were used, and Substance P, Acetate salt hydrate (Sigma aldrich, S6883) was used as a hair loss inducer, and Caffeine was used as a positive control. Each mRNA expression level is shown in Table 10 and Figure 7, Table 11 and Figure 8.

test group DKK-1 mRNA expression rate (%) Stdev. Non-irradiated group (Control) 51.18 4.45 Substnace P (0.1 nM) 100.00 0.00 Substnace P (0.1 nM) + Caffeine (1 ppm) 44.06 1.60 Substnace P (0.1 nM) +
Preparation Example 1: Buckwheat sprout 70% (w / w) ethanol aqueous solution extract
(100 ppm) 19.37 1.07 Substnace P (0.1 nM) +
Preparation Example 5: Homoorientin (10 ppm) 43.76 1.68

test group IL-6 mRNA expression rate (%) Stdev. Non-irradiated group (Control) 36.09 4.60 Substnace P (nM) 100.00 0.00 Substnace P (0.1 nM) + Caffeine (1 ppm) 77.64 8.05 Substnace P (0.1 nM) +
Preparation Example 1: Buckwheat sprout 70% (w / w) ethanol aqueous solution extract
(100 ppm) 38.37 9.50 Substnace P (0.1 nM) +
Preparation Example 5: Homoorientin (10 ppm) 76.96 5.99

Compared to the condition treated with Substnace P under stress-related hair loss conditions, the 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts inhibited the expression of DKK-1, which causes hair loss by suppressing Wnt protein function, by 80.6%, and inhibited the expression of hair roots by inducing inflammation. It has the effect of alleviating hair loss caused by stress by inhibiting 61.6% of the gene expression of IL-6 that induces hair loss by killing was inhibited by 56.2%, and the gene expression of IL-6 was also inhibited by 23%, confirming that there was an effect of alleviating hair loss due to stress.

<Experimental Example 8. Investigation of DKK-1 and IL-6 expression levels of buckwheat sprout 70% (w/w) ethanol aqueous solution extract and indicator homo orientin caused by fine dust>

Particular matter 10 (PM10-like, PAH's, ERMCZ100, Sigma-Aldrich, Germany) was treated as a fine dust sample to hair hair cells to evaluate the effect of mitigating hair loss caused by fine dust, and TGF-β2, IL-6, β- Actin gene expression experiments were performed through real time PCR. Caffeine was used as a positive control. The mRNA expression levels of each gene are shown in Table 12 and Table 13.

test group DKK-1 mRNA expression rate (%) Stdev. Non-irradiated group (Control) 48.13 4.05 PM10 (100 ppm) 100.00 0.00 PM10 (100 ppm) + Caffeine (1 ppm) 79.90 3.88 PM10 (100ppm) +
Preparation Example 1: Buckwheat sprout 70% (w / w) ethanol aqueous solution extract (100 ppm) 83.36 9.29 PM10 (100ppm) + Preparation Example 5: Homoorientin (10ppm) 90.66 5.67

test group IL-6 mRNA expression rate (%) Stdev. Non-irradiated group (Control) 42.68 0.62 PM10 (100 ppm) 100.00 0.00 PM10 (100 ppm) + Caffeine (1 ppm) 83.56 5.60 PM10 (100ppm) +
Preparation Example 1: Buckwheat sprout 70% (w / w) ethanol aqueous solution extract (100 ppm) 59.30 4.73 PM10 (100ppm) + Preparation Example 5: Homoorientin (10ppm) 86.73 2.57

In order to alleviate the symptoms of hair loss due to fine dust, the 70% (w/w) ethanol aqueous solution extract of buckwheat sprouts under the condition that particular matter 10 (PM10) was treated in the hair cell line showed the expression of DKK-1, which causes hair loss by suppressing Wnt protein function. Inhibits 16.6% of hair loss and kills hair roots through induction of inflammation, thereby inhibiting 40.7% of the gene expression of IL-6 that induces hair loss, thereby alleviating hair loss caused by fine dust. At a concentration 10 times lower than the extract, it was confirmed that there was an effect in alleviating hair loss due to fine dust by inhibiting the expression of DKK-1 by 9.3% as well as the expression of IL-6 by 13.3%.

<Experimental Example 9. Confirmation of hair root strengthening-related gene HGF and hair growth-related gene KGF in dermal papilla cells or hair cells>

Each test sample was treated with a concentration of 100 ppm (10 ppm for homo orientin, Preparation Example 5) to dermal papilla cells or hair cells, and the growth degree of hair cells was compared with that of the non-irradiated group. However, minoxidil and caffeine were tested at 1 ppm as in the previous experiment, and the group not performing the experiment was marked with '-'.

sample HGF mRNA expression rate in dermal papilla cells (%) KGF mRNA expression rate in mother cells (%) non-irradiation group 100.00 100.00 minoxidil 114.71 - Caffeine - 119.23 Preparation Example 1 128.21 128.25 Preparation 2 232.31 210.42 Preparation 3 131.22 125.23 Preparation 4 135.33 123.42 Preparation 5 121.81 132.22 Comparative Preparation Example 1 105.46 104.52 Comparative Preparation Example 2 106.13 102.32 Comparative Preparation Example 3 103.64 103.45 Comparative Preparation Example 4 104.32 106.62 Comparative Preparation Example 5 101.40 103.36 Comparative Preparation Example 6 104.53 102.52 Comparative Preparation Example 7 102.64 103.23 Comparative Preparation Example 8 103.20 105.14 Comparative Preparation Example 9 105.35 110.21 Comparative Preparation Example 10 102.12 104.57 Comparative Preparation Example 11 103.23 105.69 Comparative Preparation Example 12 103.32 106.12 Comparative Preparation Example 13 105.43 102.21

<Experimental Example 10. Androgen receptor in male pattern hair loss model Check the expression level>

As in the conditions of Experimental Example 6, each test sample was treated in dermal papilla cells at a concentration of 100 ppm (homo-orientin, Preparation Example 5, 10 ppm), and the cell Androgen receptor expression level was compared with 5α-Dihydrotestosterone (DHT) 50 nM treatment group. . Minoxidil was tested at 1ppm as in the previous experiment, and the group not performing the experiment was marked with '-'.

sample in dermal papilla cells
Androgen receptor mRNA expression rate (%) non-irradiation group 65.32 DHT 100.00 DHT + Minoxidil 58.21 DHT + Preparation Example 1 44.05 DHT + Preparation Example 2 32.22 DHT + Preparation Example 3 56.09 DHT + Preparation Example 4 61.77 DHT + Preparation Example 5 34.40 DHT + Comparative Preparation Example 1 89.33 DHT + Comparative Preparation Example 2 79.24 DHT + Comparative Preparation Example 3 78.56 DHT + Comparative Preparation Example 4 87.62 DHT + Comparative Preparation Example 5 92.30 DHT + Comparative Preparation Example 6 84.04 DHT + Comparative Preparation Example 7 82.84 DHT + Comparative Preparation Example 8 79.62 DHT + Comparative Preparation Example 9 83.45 DHT + Comparative Preparation Example 10 78.34 DHT + Comparative Preparation Example 11 89.26 DHT + Comparative Preparation Example 12 93.50 DHT + Comparative Preparation Example 13 91.23

<Experimental Example 11. Confirmation of DKK-1 expression level in stress hair loss model>

As in the conditions of Experimental Example 7, each test sample was treated in mother cells at a concentration of 100 ppm (homo-orientin, Preparation Example 5, 10 ppm), and the DKK-1 expression level of the cells was compared with that of the Substnace P 0.1nM treatment group. Caffeine was tested at 1ppm as in the previous experiment, and the group not performing the experiment was marked with '-'.

sample DKK-1 mRNA expression rate (%) in parental cells non-irradiation group 54.21 Substnace P 100.00 Substnace P + Caffeine 45.21 Substnace P + Preparation Example 1 13.23 Substnace P + Preparation Example 2 11.43 Substnace P + Preparation Example 3 18.23 Substnace P + Preparation Example 4 19.13 Substnace P + Preparation Example 5 15.23 Substnace P + Comparative Preparation Example 1 87.14 Substnace P + Comparative Preparation Example 2 83.25 Substnace P + Comparative Preparation Example 3 75.56 Substnace P + Comparative Preparation Example 4 92.08 Substnace P + Comparative Preparation Example 5 74.50 Substnace P + Comparative Preparation Example 6 88.35 Substnace P + Comparative Preparation Example 7 72.28 Substnace P + Comparative Preparation Example 8 84.83 Substnace P + Comparative Preparation Example 9 96.45 Substnace P + Comparative Preparation Example 10 89.21 Substnace P + Comparative Preparation Example 11 82.97 Substnace P + Comparative Preparation Example 12 81.51 Substnace P + Comparative Preparation Example 13 83.34

<Experimental Example 12. Confirmation of expression level in fine dust-induced hair loss model>

As in the conditions of Experimental Example 8, each test sample was treated in mother cells at a concentration of 100 ppm (homo-orientin, Preparation Example 5, 10 ppm), and the DKK-1 expression level of the cells was compared with the PM10 (particular matter 10) 100 ppm group. Caffeine was tested at 1ppm as in the previous experiment, and the group not performing the experiment was marked with '-'.

sample in mother cells
DKK-1 mRNA expression rate (%) non-irradiation group 51.12 PM10 100.00 PM10 + Caffeine 74.32 PM10 + Preparation Example 1 72.15 PM10 + Preparation Example 2 66.52 PM10 + Preparation Example 3 74.06 PM10 + Preparation Example 4 75.93 PM10 + Production Example 5 76.81 PM10 + Comparative Preparation Example 1 93.49 PM10 + Comparative Preparation Example 2 93.33 PM10 + Comparative Preparation Example 3 94.24 PM10 + Comparative Preparation Example 4 92.52 PM10 + Comparative Preparation Example 5 97.65 PM10 + Comparative Preparation Example 6 94.06 PM10 + Comparative Preparation Example 7 93.39 PM10 + Comparative Preparation Example 8 94.48 PM10 + Comparative Preparation Example 9 89.32 PM10 + Comparative Preparation Example 10 90.83 PM10 + Comparative Preparation Example 11 93.54 PM10 + Comparative Preparation Example 12 93.25 PM10 + Comparative Preparation Example 13 95.32

<Experimental Example 13. Evaluation of hair root strengthening efficacy with other sprout extracts>

According to the conditions of Tables 18 and 19 below, the hair root strengthening effect of 70% (w / w) ethanol aqueous solution extract of buckwheat sprout and homo orientin compound isolated therefrom was 70% (w / w) for oat sprouts or barley sprouts. ) In comparison with the aqueous ethanol solution, each result was confirmed in the dermal papilla cells, which are also shown in FIGS. 14 and 15 .

hair bulb strength HGF Sample Name Average Stdev. p-value Control 100.00 0.00 1.000 Minoxidil (1ppm) 114.74 9.20 0.050 Preparation Example 1: 70% ethanol aqueous solution extract of buckwheat sprout (100ppm) 126.40 6.98 0.003 Preparation Example 5: Homoorientin (10ppm) 121.81 1.37 0.000 Comparative Preparation Example 14: 70% ethanol aqueous solution extract of sprouted oats (100ppm) 78.47 3.42 0.000 Comparative Preparation Example 15: 70% ethanol aqueous solution extract of sprouted barley (100ppm) 65.67 3.21 0.000

hair bulb strength VEGF Sample Name Average Stdev. p-value Control 100.00 0.00 1.000 Minoxidil (1ppm) 114.97 4.27 0.004 Preparation Example 1: 70% ethanol aqueous solution extract of buckwheat sprout (100ppm) 171.68 1.38 0.000 Preparation Example 5: Homoorientin (10ppm) 134.14 1.61 0.000 Comparative Preparation Example 14: 70% ethanol aqueous solution extract of sprouted oats (100ppm) 129.51 4.44 0.000 Comparative Preparation Example 15: 70% ethanol aqueous solution extract of sprouted barley (100ppm) 125.48 1.84 0.000

As a result, even with the same sprout extract, it can be confirmed that the hair growth function is significantly better in buckwheat sprouts.

<Cosmetic Composition Formulation Example 1. Shampoo>

A shampoo for preventing hair loss was prepared by mixing each raw material with the content shown in Table 20 below.

material name Added amount (g) Preparation Example 1 Extract 0.005 polyquaternium 2.0 vegetable glycerin 9.5 glucamate 9.5 keratin 2.0 betaine 2.0 allantoin 1.0 panthenol 1.5 EDTA 0.5 silk amino acid 1.0 citric acid 1.0 1,2-Hexanediol 5.0 olive oil peg 5.0 Disodium Laureth Sulfosuccinate 40.0 carbomer 20.0 Diethanolamide lauric acid 20.0 Ammonium Lauryl Sulfate 20.0 isostearic acid 20.0 cetanol 20.0 Ethylene glycol monostearate 20.0 horse oil 1.0 Lauramidopropyl Betaine 30.0 Decylglucoside 30.0 camellia oil 1.0 pomegranate seed oil 1.0 shea butter 1.0 milk thistle oil 1.0 jojoba oil 1.0 cypress essential oil 1.0 tea tree essential oil 1.0 eucalyptus essential oil 1.0 Citronella essential oil 1.0 Purified water remaining amount total amount 500.0

Claims (9)

A cosmetic composition for strengthening hair roots, promoting hair growth or preventing hair loss, characterized in that it contains a buckwheat sprout extract comprising Homoorientin of the following formula (1) as an active ingredient.
[Formula 1]

According to claim 1,
The buckwheat sprout extract is a cosmetic composition for strengthening hair roots, promoting hair growth or preventing hair loss, characterized in that the buckwheat sprouts are extracted using an aqueous solution of C1-C4 alcohol as a solvent. According to claim 1,
The buckwheat sprout extract is a cosmetic composition for strengthening hair roots, promoting hair growth or preventing hair loss, characterized in that it has an inhibitory function of male pattern hair loss, stress hair loss, or hair loss caused by fine dust. A cosmetic composition for strengthening hair roots, promoting hair growth or preventing hair loss, characterized in that it contains homoorientin of the following formula (1).
[Formula 1]

5. The method of claim 4,
The homoorientin (Homoorientin) is a cosmetic composition for strengthening hair roots, promoting hair growth or preventing hair loss, characterized in that the buckwheat sprouts are extracted using an aqueous solution of C1-C4 alcohol as a solvent. 5. The method of claim 4,
The homoorientin (Homoorientin) is a cosmetic composition for strengthening hair roots, promoting hair growth or preventing hair loss, characterized in that it has an inhibitory function of male pattern hair loss, stress hair loss or hair loss caused by fine dust. A pharmaceutical composition for strengthening hair roots, promoting hair growth or preventing hair loss, characterized in that it contains a buckwheat sprout extract comprising Homoorientin of Formula 1 as an active ingredient.
[Formula 1]

A pharmaceutical composition for strengthening hair roots, promoting hair growth or preventing hair loss, characterized in that it contains homo orientin of the following formula (1).
[Formula 1]

A health functional food for strengthening hair roots, promoting hair growth or preventing hair loss, characterized in that it contains a buckwheat sprout extract containing Homoorientin of Formula 1 as an active ingredient.
[Formula 1]

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