KR102645621B1 - Composition for Regenerating Skin and Enhancing Hair Growth Comprising Apigenin - Google Patents

Abstract

The present invention relates to a composition for promoting skin regeneration and hair growth containing apigenin. More specifically, the composition for promoting skin regeneration and hair growth promotes cell growth through Wnt/beta-catenin signaling. By promoting differentiation, it can exhibit skin regeneration and hair growth promotion effects.

Description

Composition for Regenerating Skin and Enhancing Hair Growth Comprising Apigenin}

The present invention relates to a composition for promoting skin regeneration and hair growth containing apigenin.

Skin exists on the outermost layer of the human body and is in direct contact with the external environment, protecting the human body from external damage and preventing excessive evaporation of body moisture and heat. The skin is composed of three layers: epidermis, dermis, and subcutaneous tissue, and most of the outermost epidermis is composed of keratinocytes. Keratinocytes of the epidermis protect the skin by forming keratin through proliferation, migration, and differentiation from the basal layer, and participate in inflammatory and immune responses by producing various cytokines. In addition, the proliferation and migration of keratinocytes not only provide wound healing and skin regeneration, but also induce collagen synthesis to improve skin elasticity and basement membrane formation, thereby controlling the adhesion and proliferation of skin cells and harmful harmful substances. It improves the barrier function of selectively penetrating substances and improves the interaction between the epidermis and dermis. Because of this, the movement and proliferation of skin epidermal keratinocytes not only promotes skin regeneration and wound healing, but also improves skin elasticity and helps maintain healthy skin.

As interest in skin care increases, in order to maintain healthier and more beautiful skin, various highly safe natural bioactive substances derived from various animals, plants, and microorganisms have been used to maintain the skin's unique functions. Various attempts have been widely carried out to effectively inhibit or alleviate skin aging by promoting and activating skin cells.

Apigenin is a nutrient well known as an antioxidant contained in vegetables, and is used as an ingredient for skin whitening, skin regeneration, and hair growth promotion.

Korean Patent Publication No. 2017-0119757 is a cosmetic composition using apigenin, germanium, and amino acids, and discloses that it can exhibit skin regeneration and stabilization effects through a synergistic effect in which the effects of each ingredient are expressed in a complex manner.

Chinese Patent No. 106880693 discloses that it is a composition containing apigenin and ginseng extract and has hair loss prevention effects.

In this way, technology development has been steadily taking place for products related to skin regeneration or hair loss using apigenin, but consumer satisfaction with its efficacy is still low.

Therefore, there is a continuous need for research and development on products that use apigenin, which is effective in promoting skin regeneration and hair growth, and can maximize its efficacy by acting synergistically with apigenin.

China Registered Patent No. 106880693

In order to solve the above problem, the present inventors prepared a composition containing at least one of caffeine, ginseng extract, and green tea extract and apigenin, and confirmed that the composition was excellent in promoting skin regeneration and hair growth growth.

Therefore, the purpose of the present invention is to provide a composition for promoting skin regeneration and hair growth containing apigenin.

In order to achieve the above object, the present invention includes apigenin; and at least one selected from the group consisting of caffeine, ginseng extract, and green tea extract. It provides a composition for promoting skin regeneration and hair growth, including a composition.

The apigenin may be included in an amount of 40 to 60% by weight based on the total weight of the composition.

At least one selected from the group consisting of the ginseng extract and the green tea extract may be included in an amount of 40 to 60% by weight based on the total weight of the composition for skin regeneration.

The composition may activate Wnt/beta-catenin signal.

The concentration of the composition may be 10 to 40 ppm based on solid content concentration.

The composition may contain apigenin and caffeine.

The composition may include apigenin and ginseng extract.

The composition may include apigenin and green tea extract.

The composition may be one or more selected from the group consisting of cosmetic compositions, food compositions, and pharmaceutical compositions.

The composition for promoting skin regeneration and hair growth of the present invention contains apigenin and at least one of caffeine, ginseng extract, and green tea extract, thereby providing a skin regeneration effect, specifically excellent skin, due to the synergistic effect of the effects of each ingredient. It can have wound healing and scar reduction effects.

In addition, the composition for promoting skin regeneration and hair growth has the effect of preventing or improving hair loss by promoting hair growth.

In addition, the composition for promoting skin regeneration and hair growth promotes cell differentiation through Wnt/beta-catenin signaling, and thus can further improve the effects of promoting skin regeneration and hair growth.

In addition, the composition for promoting skin regeneration and hair growth can be widely applied to cosmetic compositions, food compositions, and pharmaceutical compositions that have skin regeneration and hair growth promoting effects.

Figure 1 is a graph showing the Wnt signaling activity of compositions prepared in Examples and Comparative Examples.

Hereinafter, the present invention will be described in detail so that those skilled in the art can easily implement it. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein.

Composition for promoting skin regeneration and hair growth

The present invention relates to a composition for promoting skin regeneration and hair growth, wherein the composition for promoting skin regeneration and hair growth includes apigenin; and at least one selected from the group consisting of caffeine, ginseng extract, and green tea extract.

At least one selected from the group consisting of apigenin, caffeine, ginseng extract, and green tea extract each has the effect of promoting skin regeneration or hair growth, but when combined, the synergistic effect of these ingredients promotes skin regeneration or hair growth. The promotion efficacy can be significantly improved.

Preferably, the composition for promoting skin regeneration and hair growth may contain apigenin and caffeine, or apigenin and ginseng extract, or a combination of apigenin and green tea extract. When the composition for promoting skin regeneration and hair growth includes such a combination of ingredients, the synergistic effect for promoting skin regeneration and hair growth may be the best.

In the present invention, the apigenin is a type of flavonoid component contained in fruits, vegetables, or herbs, and may be extracted from these fruits, vegetables, or herbs.

The apigenin may be included in an amount of 40 to 60% by weight, preferably 42 to 58% by weight, and more preferably 45 to 55% by weight, based on the total weight of the composition for promoting skin regeneration or hair growth. If it is less than or exceeds the above range, the synergistic effect with one or more selected from the group consisting of caffeine, ginseng extract, and green tea extract may be reduced.

In the present invention, one or more selected from the group consisting of caffeine, ginseng extract, and green tea extract may exhibit a synergistic effect for promoting skin regeneration and hair growth by combining with the apigenon.

At least one selected from the group consisting of caffeine, ginseng extract, and green tea extract is present in an amount of 40 to 60% by weight, preferably 42 to 58% by weight, more preferably, based on the total weight of the composition for promoting skin regeneration or hair growth. It may be included in 45 to 55% by weight. If it is less than or exceeds the above range, the synergistic effect with apigenon may be reduced.

The caffeine is not particularly limited, but includes synthetic caffeine; Alternatively, the form of one or more natural caffeine extracts selected from the group consisting of guarana extract, coffee extract, and tea extract can be used.

The concentration of the caffeine may be 5 to 20 ppm, preferably 5 to 15 ppm, and preferably 7 to 12 ppm, based on solid concentration. If it is less than the above range, the effect of promoting skin regeneration and hair growth may be minimal, and if it is exceeding the above range, the optimal concentration for synergy with apigenon may be exceeded, and the effect of promoting skin regeneration and hair growth may be reduced. At this time, the concentration of caffeine refers to the concentration of an aqueous caffeine solution in the case of synthetic caffeine, and the solid concentration in the case of natural caffeine extract.

The ginseng extract may be one or more selected from the group consisting of ginseng root extract, ginseng leaf extract, ginseng fruit extract, and ginseng seed extract, and is preferably ginseng root extract.

The concentration of the ginseng extract may be 5 to 20 ppm, preferably 5 to 15 ppm, and preferably 7 to 12 ppm, based on solid concentration. If it is less than the above range, the effect of promoting skin regeneration and hair growth may be minimal, and if it is exceeding the above range, the optimal concentration for synergy with apigenon may be exceeded, and the effect of promoting skin regeneration and hair growth may be reduced.

Additionally, the ginseng extract may be extracted using an extraction solvent selected from the group consisting of water, an organic solvent, and a mixed solvent of water and an organic solvent. The organic solvent may be one or more selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate, and chloroform. Preferably, the extraction solvent may be ethanol.

In addition, the method of preparing the ginseng extract is not particularly limited as long as it is a typical extraction method used in the industry, but, for example, it can be prepared by extraction at 10 to 80 ° C. for 3 to 24 hours using the extraction solvent. there is.

The green tea extract may be one or more selected from the group consisting of green tea leaf extract, green tea flower extract, and green tea stem extract, and is preferably green tea leaf extract.

The green tea extract may have a concentration of 5 to 20 ppm, preferably 5 to 15 ppm, and preferably 7 to 12 ppm, based on solid content concentration. If it is less than the above range, the effect of promoting skin regeneration and hair growth may be minimal, and if it is exceeding the above range, the optimal concentration for synergy with apigenon may be exceeded, and the effect of promoting skin regeneration and hair growth may be reduced.

Additionally, the green tea extract may be a hot water extract or a C ₁ to C ₅ lower alcohol extract. Preferably, the green tea extract may be an ethanol extract.

Additionally, the method for producing the green tea extract is not particularly limited as long as it is a typical extraction method used in the art. For example, if the green tea extract is a hot water extract, it can be obtained through the process of adding green tea, hot water extraction, filtration, concentration under reduced pressure, and spray drying. Alternatively, if the green tea extract is an ethanol extract, it can be prepared by extraction using ethanol at 10 to 80 ° C. for 3 to 24 hours.

In the present invention, the concentration of the composition for promoting skin regeneration and hair growth may be 10 to 40 ppm, preferably 10 to 30 ppm, and more preferably 15 to 25 ppm, based on solid content concentration. If it is less than or exceeds the above range, the effects of promoting skin regeneration and hair growth may be reduced. In addition, when the composition is in the above concentration range, it can enable activation of Wint/beta-catenin signal as described later.

In addition, the composition for promoting skin regeneration and hair growth can promote skin regeneration and hair growth by activating Wnt/beta-catenin signal.

Wnt/beta-catenin signaling is a signal transduction system related to the development of living organisms, and is known to play a role in the development of skin and hair follicles. Furthermore, it is known that when a wound occurs on the skin, Wnt signaling is essentially expressed during the skin tissue recovery process to promote differentiation of epithelial cells (Fathke et al ., "Wnt signaling induces epithelial differentiation during cutaneous wound healing", BMC Cell Biology 2006, 7:4). Therefore, when Wint signaling is promoted, the differentiation of epithelial cells can be promoted, which can promote skin regeneration and further promote the healing of skin wounds. Gardenia extract promotes Wnt/beta-catenin signaling in stem cells. Therefore, agents containing it can promote skin regeneration, and specifically promote healing of skin wounds (Kateri A. Moore et al ., "Stem Cells and Their Niches", Science, VOL 311, p1880-1885 ).

In the present invention, the composition for promoting skin regeneration and hair growth may be at least one selected from the group consisting of cosmetic compositions, food compositions, and pharmaceutical compositions.

When the composition for promoting skin regeneration and hair growth is a cosmetic composition, it may be formulated containing a cosmetically or dermatologically acceptable medium or base. In addition, the cosmetic composition for promoting skin regeneration and hair growth may be provided in any formulation suitable for topical application, for example, a solution, an emulsion obtained by dispersing an oil phase in an oil phase, an emulsion obtained by dispersing an oil phase in an oil phase, a suspension, It may be provided in the form of a solid, gel, powder, paste, foam or aerosol composition. Compositions of this dosage form can be prepared according to conventional methods in the art.

In addition, the cosmetic composition for promoting skin regeneration and hair growth may contain, in addition to the above-mentioned substances, other ingredients that can preferably have a synergistic effect on the main effect, within a range that does not impair the main effect. In addition, the cosmetic composition for promoting skin regeneration and hair growth may further include moisturizers, emollients, ultraviolet absorbers, preservatives, disinfectants, antioxidants, pH adjusters, organic and inorganic pigments, fragrances, cooling agents, or antiperspirants. The mixing amount of the above ingredients can be easily selected by a person skilled in the art within the range that does not impair the purpose and effect of the present invention, and the mixing amount may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight, based on the total weight of the composition. .

When the composition for promoting skin regeneration and hair growth is a food composition, it can be applied to various types of food additives or functional foods. The food composition for promoting skin regeneration and hair growth can be processed into fermented milk, cheese, yogurt, juice, probiotics, health supplements, etc., and can be used in the form of various other food additives.

In addition, the food composition for promoting skin regeneration and hair growth may contain other ingredients that can have a synergistic effect on the main effect within a range that does not impair the main effect aimed at by the present invention. For example, to improve physical properties, additives such as fragrances, pigments, disinfectants, antioxidants, preservatives, moisturizers, thickeners, inorganic salts, emulsifiers, and synthetic polymers may be further included. In addition, it may further contain auxiliary ingredients such as water-soluble vitamins, oil-soluble vitamins, high molecular weight peptides, high molecular weight polysaccharides, and seaweed extract. The above ingredients can be appropriately selected and mixed by a person skilled in the art according to the formulation or purpose of use without difficulty, and the amount added can be selected within a range that does not impair the purpose and effect of the present invention. For example, the amount of the ingredients added may be in the range of 0.01 to 5% by weight, more specifically 0.01 to 3% by weight, based on the total weight of the composition.

The food composition for promoting skin regeneration and hair growth according to the present invention can be formulated in various forms such as solutions, emulsions, viscous mixtures, tablets, and powders, and can be used for simple drinking, injection, spraying, or squeezing. It can be administered in a variety of ways.

If the composition for promoting skin regeneration and hair growth is a pharmaceutical composition, it can be applied to pharmaceuticals, and can be administered as an oral administration agent or in the form of a solid, semi-solid or liquid by adding an inorganic or organic carrier commonly used in the active ingredient used in the present invention. It can be formulated as a parenteral administration, and the pharmaceutical composition according to the present invention can be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, etc.

Formulations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups, pellets, etc. In addition, dosage forms for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, etc. The composition according to the present invention can be easily formulated with active ingredients by carrying out conventional methods, and at this time, surfactants, excipients, colorants, spices, preservatives, stabilizers, buffers, suspending agents, and other commonly used auxiliaries can be appropriately used. there is.

In addition, the administered dose of the active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, gender, weight, pathological condition and severity of the subject, administration route, or the judgment of the prescriber. Determination of an appropriate dosage based on these factors is within the level of one skilled in the art, and the daily dosage may be, for example, 0.1 mg/kg/day to 100 mg/kg/day, more specifically 5 mg/kg/day to 50 mg/kg. /It may be work, but is not limited to this.

The composition for promoting skin regeneration and hair growth as described above contains at least one of caffeine, ginseng extract, and green tea extract along with apigenin, thereby providing a skin regeneration effect, specifically an excellent skin regeneration effect, due to the synergistic effect of the effects of each ingredient. It has the effect of healing skin wounds and reducing scars, and has the effect of promoting hair growth, so it can be widely applied in the fields of cosmetics, food, and pharmaceuticals with these effects.

Preferred examples are presented below to aid understanding of the present invention. However, the following examples are merely illustrative of the present invention, and it is obvious to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention. , it is natural that such changes and modifications fall within the scope of the attached patent claims.

Preparation Example 1: Preparation of Apigenin

Thistle (Circium japonicum) was collected, dried, and used in a mountainous area in North Chungcheong Province. The product purchased from Gyeongdong Market (Seoul, Korea) was dried and used. Dried thistle (Circium japonicum) was extracted by adding 95% alcohol and concentrated using a reduced pressure concentrator to prepare a crude alcohol extract.

The crude alcohol extract was dissolved in distilled water and ethyl acetate and mixed at a ratio (v/v) of 1:1. Distribution was repeated three times, and the ethyl acetate extract was reduced in pressure, concentrated, and dried.

The dried product was subjected to silica gel flash chromatography (solvent system: chloroform/ethyl acetate = 2:1), and the active fraction was dried again under reduced pressure, then dissolved in a small amount of methanol to saturation and reduced to millipore (0.45 nm). After filtration, the active substance, apigenin, was fractionated using a high-performance analysis device (HPLC, column: ODS 20×250 nm; 80% methanol).

Preparation Example 2: Preparation of caffeine

Caffeine was purchased from Sigma Chemicals, Inc. (St. Louis, MO, USA).

Preparation Example 3: Preparation of ginseng extract

1 kg of dried white ginseng was ground in a blender, 5 liters of purified water was added thereto, extracted by stirring for 24 hours in hot water at 38-42°C, and then soaked at 15°C for 1 day. Afterwards, the residue and the filtrate were separated through filter cloth filtration and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 270 g of the extract.

Preparation Example 4: Preparation of green tea extract

1 kg of green tea leaves that were first processed (grinding process) were crushed in a blender, then 5 liters of purified water was added, stirred and extracted with hot water at 38-42°C for 24 hours, and then soaked at 15°C for 1 day. Afterwards, the residue and the filtrate were separated through filter cloth filtration and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 290 g of the extract.

Example 1

50% by weight of apigenin and 50% by weight of caffeine prepared in Preparation Example 1 and Preparation Example 2, respectively, were mixed to prepare a composition of 20 ppm.

Example 2

50% by weight of apigenin and 50% by weight of ginseng extract prepared in Preparation Example 1 and Preparation Example 3, respectively, were mixed to prepare a composition of 20 ppm.

Example 3

50% by weight of apigenin and 50% by weight of green tea extract prepared in Preparation Example 1 and Preparation Example 3, respectively, were mixed to prepare a composition of 20 ppm.

Comparative Examples 1 to 8

For Comparative Examples 1 to 8, compositions as shown in Table 1 below were prepared at 20 ppm, respectively.

composition Example 1 Apigenin + Caffeine Example 2 Apigenin + Ginseng Extract Example 3 Apigenin + green tea extract Comparative Example 1 Control Comparative example 2 LiCl Comparative Example 3 Apigenin Comparative Example 4 Caffeine Comparative Example 5 Ginseng Extract Comparative Example 6 green tea extract Comparative Example 7 Madecassoside Comparative example 8 Apigenin + Madecassoside

Experimental Example 1

When a Wnt signal is given to epidermal stem cells, the protein complex (GSK3, APC, Axin) that suppresses intracellular beta-catenin is inhibited, causing beta-catenin to move into the cell nucleus and participate in development and hair growth. Using the principle that various genes are expressed, experiments were conducted on the degree of Wnt signal transduction by the compositions of Examples and Comparative Examples. The specific experimental method is as follows.

[Wnt signaling experiment]

(1)Cell culture

After purchasing the Wnt reporter cell line (#ENZ-61002-0001), the Wnt reporter cell line was seeded at 1x10 ⁴ cells/well in a white/clear flat bottom 96-well plate coated with Collagen type I.

(2) Material handling

100 ㎕ of each concentration of the compositions of Examples and Comparative Examples was treated for 24 hours using serum free medium.

As a positive control, 10 mM LiCl was used (depending on the cell state, 10 mM shows an activity of 150-300% compared to the control, and 50mM shows an activity of 1000-4000% compared to the control).

(3) Luciferase activity measurement

100 ㎕ of ONE-Glo™ Luciferase Assay reagent was added to each well and reacted at room temperature for 3 minutes with light blocked.

Activity was measured using a Wallac Victor2 multilabel counter.

(4) Data processing method

Based on the absorbance of the control group that was not treated with the material, the luminescence intensity of the group treated with the material compared to the control group was converted into a % value and the value obtained was taken as the response ratio (%). The higher the response ratio value, the better the Wnt signaling activity.

Figure 1 is a graph showing the Wnt signaling activity of compositions prepared in Examples and Comparative Examples.

Referring to Figure 1, the composition containing the combination of apigenin and caffeine in Example 1, the combination of apigenin and ginseng extract in Example 2, and the combination of apigenin and green tea extract in Example 3 have Wnt signaling activity. You can tell it's excellent.

On the other hand, a composition containing apigenin, caffeine, ginseng extract, green tea extract, and madecassoside alone, as in Comparative Examples 3 to 7, and a combination of apigenin and madecassoside, as in Comparative Example 8. It can be seen that the Wnt signaling activity of the composition is significantly reduced compared to Examples 1, 2, and 3.

From this, the composition containing the combination of apigenin and caffeine in Example 1, the combination of apigenin and ginseng extract in Example 2, and the combination of apigenin and green tea extract in Example 3 have a synergistic effect due to the combination of each component. As a result, it was confirmed that Wnt signaling efficacy was significantly improved.

Although the present invention has been described above with limited examples and drawings, the present invention is not limited thereto, and the technical idea of the present invention and the following description will be provided by those skilled in the art in the technical field to which the present invention pertains. Of course, various modifications and variations are possible within the scope of equivalence of the patent claims.

Claims (9)

apigenin; and at least one selected from the group consisting of caffeine, ginseng extract, and green tea extract. A composition for skin regeneration comprising a. According to paragraph 1,
A composition for skin regeneration, wherein the apigenin is contained in an amount of 40 to 60% by weight based on the total weight of the composition. According to paragraph 1,
A composition for skin regeneration, wherein at least one selected from the group consisting of caffeine, ginseng extract, and green tea extract is contained in an amount of 40 to 60% by weight based on the total weight of the composition for skin regeneration. According to paragraph 1,
The composition is a composition for skin regeneration that activates Wnt/beta-catenin signal. According to paragraph 1,
A composition for skin regeneration, wherein the concentration of the composition is 10 to 40 ppm based on solid content concentration. According to paragraph 1,
A composition for skin regeneration comprising apigenin and caffeine. According to paragraph 1,
A composition for skin regeneration comprising apigenin and ginseng extract. According to paragraph 1,
A composition for skin regeneration comprising apigenin and green tea extract. According to paragraph 1,
The composition is a composition for skin regeneration, which is at least one selected from the group consisting of cosmetic compositions, food compositions, and pharmaceutical compositions.