KR101008954B1 - a cosmetic composition, a pharmaceutical composition comprising fermented product from acai berry and a method for fermentating acai berry - Google Patents

Abstract

A cosmetic composition, a pharmaceutical composition, and a method of fermenting an asyra are provided.

Cosmetic composition according to the present invention, the pharmaceutical composition is a fermented product of Ascai berry (Acai berry) as an active ingredient, because it has excellent UV protection, whitening, anti-aging and antioxidant effects, in particular as a functional ingredient of cosmetics for improving skin function It is very suitable and the effect is also excellent. Furthermore, since the composition containing the fermented product of assay berry has an excellent effect in treating inflammation caused by ultraviolet rays, it is also excellent as a therapeutic agent for skin inflammation caused by excessive ultraviolet exposure.

Description

Cosmetic composition, pharmaceutical composition and fermented product from acai berry and a method for fermentating acai berry}

The present invention relates to a cosmetic composition, a pharmaceutical composition, and a fermentation method of assay berry comprising a fermentation product of Acai berry, and more particularly, to an assay berry having excellent sunscreen, whitening, anti-aging and antioxidant effects. The present invention relates to a fermentation method of a cosmetic composition, a pharmaceutical composition and an assay berry containing a fermentation product.

With the recent advent of an aging society and the spread of well-being trends, there is an increasing demand for maintaining health and beauty for a long time. Interest in materials is increasing.

Natural plant materials have been used for a long time in the cosmetics industry because they are safer and more environmentally friendly than synthetic materials. Recently, herbal cosmetics using herbal medicine with strong physiological activity has become the mainstream of cosmetic products. Herbal cosmetics have been used for hundreds of years and have been proven in terms of safety and effectiveness, so they can be easily recognized and used by consumers, and the effect is inferior to synthetic materials. In addition, natural plant materials or herbal medicines contain various biologically active substances and antioxidants and have various effects such as anti-aging, anticancer, anti-inflammatory, and immune function improvement.

There are many substances that have excellent physiological activity on the skin and have anti-aging and whitening effects. Retinol, arbutin and vitamin C are known to be effective for wrinkle improvement and whitening as a representative functional material. However, most of these attempts to find a material that is less stable, ineffective or cause side effects in the formulation and replace it are an essential part of cosmetic material research. Recently, attempts have been made to search for substances with excellent physiological activity in natural plant materials, not synthetic ones, and plants and herbal medicines that have useful effects on skin, such as anti-aging, anti-inflammatory, and whitening, are found one after another. .

In particular, the Applicant paid attention to Acai berry, which is a fruit of Euterpe oleracea, a kind of palm tree, which grows in low wetlands and is called the tree of life.

Assay trees have been used by people in the region for hundreds of years, especially the Anomabi Indians, who ate a lot before hunting, and are known to be high-energy foods that will last three days after eating asyberry. Asyra berry is also prescribed to help mothers recover quickly. Assay also contains eight essential amino acids, which are rich in calcium, vitamin E, iron and magnesium compared to milk. For this reason, it is estimated to be close to complete food.

However, the research and prior art of the conventional assay is limited to the study on the material extracted from the asyberry. Korean Patent Laid-Open Publication No. 10-2008-0001075 discloses an anti-wrinkle effect of an extract extracted from Assy, but various effects of Assyberry and various methods of improving the same are not disclosed at all.

Therefore, the first problem to be solved by the present invention is to provide a cosmetic composition as an active ingredient fermented product of assay berry.

The second problem to be solved by the present invention is to provide a pharmaceutical composition comprising the fermented product of assay berry as an active ingredient.

The third problem to be solved by the present invention is to provide a fermentation method that can produce a fermented product of assay berry showing an excellent effect as an active ingredient of a cosmetic composition or a pharmaceutical composition.

In order to solve the first problem, the present invention provides a cosmetic composition comprising the fermented product of Acai berry as an active ingredient. The fermented product is fermented by the lactic acid bacteria or yeast, or a combination thereof, the extract of the asy berry, the lactic acid bacteria is Lactobacillus sakei B2-16 ), Lactobacillus brevis ), Lactobacillus plantarum P23 ), Lactococcus lactis A164 , Bacillus coagulum coagulance ) may be one or more strains selected from the group consisting of. In addition, the active ingredient is 0.05% to 95% by weight of the total cosmetic composition, the cosmetic composition may be one formulation selected from the group consisting of soaps, lotions, creams, packs and cosmetic liquids.

In order to solve the second problem, the present invention provides a pharmaceutical composition for treating the skin using the fermented product of Ascai berry (Acai berry) as an active ingredient.

In order to solve the third problem, the present invention comprises the steps of obtaining an extract from an asy berry; Inoculating the obtained extract of Assybery berry or yeast or a mixed strain thereof; And it provides a fermentation method of the assay berry (Acai berry) comprising the step of culturing the extract of the inoculated assay berry to produce a fermentation product. The inoculation step of the inoculation step of the lactic acid bacteria or yeast or mixed strains of the inoculation amount may be 10 ⁵ to 10 ⁸ cfu / g or 10 ⁵ to 10 ⁸ cfu / ㎖ initial inoculation, the production step of the fermentation product is 15 to 40 It may be carried out for 18 to 120 hours at a temperature of ℃.

The cosmetic composition of the present invention using the fermented product of assay berry has excellent UV blocking, whitening, anti-aging and antioxidant effects, and is particularly suitable as a functional ingredient of cosmetics for improving skin function, and its effect is also excellent. . Furthermore, since the composition containing the fermented product of assay berry has an excellent effect in treating inflammation caused by ultraviolet rays, it is also excellent as a therapeutic agent for skin inflammation caused by excessive ultraviolet exposure.

Hereinafter, the present invention will be described in more detail with reference to the drawings and examples. However, the present invention is not limited by the following drawings and examples.

As described above, the present invention provides a cosmetic composition comprising the fermented product of asyra berry as an active ingredient. Herein, the fermentation product of the assay berry is a fermentation product obtained by fermenting the extract obtained from the assay berry. In general, fermentation is a process of preparing a substance useful for human life by decomposing organic matter using an enzyme possessed by a microorganism. The fermented product obtained by fermenting the extract from asyra with lactic acid bacteria or yeast or mixed strains thereof may exhibit a very good effect on reducing the toxicity of skin cells, and especially when fermenting the asyberry extract with the mixed strain has better efficacy. This is illustrated in more detail through the following evaluation examples.

The lactic acid bacteria is Lactobacillus sakei B2-16 ), Lactobacillus brevis ), Lactobacillus plantarum P23 ), Lactococcus lactis A164 , Bacillus coagulum coagulance ) may be one or more strains selected from the group consisting of. The type of the strain is selected in consideration of not only fermentation power but also human stability since the composition according to the present invention is directly applied to the human body in the form of an external preparation for skin, and the like, which is used in the art as long as human stability and fermentation power are recognized. Lactic acid bacteria may also be used, and the present invention is not limited to the type of lactic acid bacteria.

 In addition, the assay berry fermentation may be 0.05% to 95% by weight of the total cosmetic composition, if the asyberry ferment is less than the above range, the skin function improvement effect is insignificant, on the contrary, if more than the above range is uneconomical, It is difficult to expect various effects depending on the other composition components added to the composition. That is, the cosmetic composition according to the present invention may contain other ingredients and the like that can give a synergistic effect to the main effect within the range that does not impair the main effect by the active ingredient, for example, fragrance, In addition to additives such as pigments, fungicides, antioxidants, preservatives, humectants, thickeners, inorganic salts, emulsifiers, synthetic polymers, and the like, as well as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides and seaweed extracts It may further comprise an auxiliary component. The components may be appropriately selected and blended by those skilled in the art according to the formulation or purpose of use, and the addition amount may be selected within a range that does not impair the object and effect of the present invention, but 0.01 to 5 weight based on the total weight of the composition. %, Preferably 0.01 to 3% by weight. Therefore, if the content of the active ingredient is more than 95% by weight, such a functional ingredient is difficult to add.

In addition, the cosmetic composition comprising an assay berry fermentation as an active ingredient is a cosmetic composition that can be used for the purpose of skin moisturizing, anti-aging, whitening, anti-inflammatory or sun protection. That is, the present invention is based on the remarkable effect of improving skin efficacy obtained from asyberry berry fermentation, which will be described in more detail through the following evaluation example.

The cosmetic composition according to the present invention may be one formulation selected from the group consisting of soaps, lotions, creams, packs, and cosmetic liquids. That is, the composition according to the present invention may be included in soap, lotion, cream, pack and cosmetic liquid, which is preferably in the form of external skin, and may be applied to the skin. However, this is only one example, and cosmetics of any formulation including the cosmetic composition according to the present invention are within the scope of the present invention, and the present invention is not limited to the types of the formulations listed above. For example, it may be administered in various forms such as a solution, an emulsion, a viscous mixture, a tablet, a powder, or the like, in a variety of ways such as simple drinking, injection, spray or squeeze.

In order to solve the second problem, it provides a composition for treating the skin as an active ingredient assay berry fermentation. Since the composition for treating skin according to the present invention is particularly excellent for treating skin inflammation caused by excessive irradiation of ultraviolet rays, it can be usefully used to treat various skin diseases and skin inflammation caused by excessive tanning.

The cosmetic composition according to the present invention, the fermented product of asyra berry as an active ingredient of the pharmaceutical composition is to obtain an extract from asyra berry; Inoculating the obtained extract of Assybery berry or yeast or a mixed strain thereof; And it provides a fermentation method of the assay berry (Acai berry) comprising the step of culturing the extract of the inoculated assay berry to produce a fermentation product. The method for obtaining the extract may be used in any method commonly used in the art, and the form of the extract may be water soluble or useful.

The inoculation step is a step of injecting the strain required for fermentation to the obtained extract, in one embodiment of the present invention uses the lactic acid bacteria or yeast or a mixed strain thereof as the strain. In particular, as can be seen through the following experimental example, the fermented product obtained using the mixed strain has a unique effect having a very good skin function improvement effect. The inoculation amount of the strain may be 10 ⁵ to 10 ⁸ cfu / g or 10 ⁵ to 10 ⁸ cfu / ㎖ initial inoculation, the production step of the fermentation may be performed for 18 to 120 hours at a temperature of 15 to 40 ℃ Can be. If the number of bacteria is less than the above range is required a long fermentation process, if it exceeds the above range may be difficult to control the fermentation process due to the excessively short fermentation process. In addition, the temperature range and time range is a numerical range that can achieve a stable fermentation process control and economical efficiency, if out of the numerical range may be uneconomical or difficult to control the fermentation process.

Hereinafter, the present invention will be described in more detail with reference to Examples and Evaluation Examples. However, these examples and evaluation examples are intended to explain the present invention in more detail, and the scope of the present invention is not limited by these examples and evaluation examples according to the gist of the present invention is common knowledge in the art. It will be self-evident for those who have.

Example  One

Example  1-1

Assy Berry's  Extract obtained

Assy berry dried was mixed with water to 10% by dry weight, and then extracted for 3 to 5 hours in the range of 25 ℃ ~ 100 ℃ filtered and dried.

Example  1-2

Assy Berry's Fermented products  Produce

Assy berry extract of Example 1-1 was filtered by 0.2 mm filter. Lactobacillus Lactobacillus in the Water-Soluble Extract plantarum P23 ) was inoculated so that the initial inoculation was 10 ⁵ to 10 ⁸ cfu / g, and then cultured for 3 days at 30 ℃ and centrifuged to obtain a fermentation supernatant.

Example  2

The fermented product of asyraberry was prepared in the same manner as in Example 1, except that the inoculated strain was inoculated so as to have a mixed strain (1: 1) of lactic acid bacteria and yeast so that the initial inoculation number was 10 ⁵ to 10 ⁸ cfu / g. Was prepared .

Comparative example

In order to compare the effect of the assay berry on the fermented product of the present invention, the assay berry extract of Example 1-1, which did not undergo the fermentation step, was prepared as a comparative example.

Evaluation example  One

By ultraviolet Skin cytotoxicity  Reduction effect

First, normal human fibroblasts were charged with DMEM (Dulbecco's Modified Eagle's Medium) medium containing penicillin (100 IU / mL), streptomycin (100 g / mL), and 10% FBS (fetal bovine serum). Culture was maintained in an incubator containing 5% carbon dioxide while maintaining the ℃. The obtained fibroblasts were dispensed into 6-well plates at 3 × 10 ⁵ cells per well and incubated for 24 hours under cell culture conditions. After irradiating the cells with 25mJ / Cm ² amount of ultraviolet light after the culture, the fermentation or extract of Examples 1, 2 and Comparative Example 1 was added to 0.004%, 0.008% of the total weight of the medium, and then cultured for 24 hours, After irradiating ultraviolet light (312nm, 25, J / cm 2) to the medium, the number of cells was measured by measuring the number of cells is shown in Figure 1 to measure the degree of apoptosis.

In addition, ascorbic acid (vitamin C) was added to 12.5 μM and 25 μM as a control, instead of adding the fermented product of the fibroblast medium (control group 1) and the assay berry according to the present invention. Fibroblast medium (control 2) was prepared to measure the degree of bacterial death according to ultraviolet irradiation, which is shown in FIG.

Referring to FIG. 1, in the case of the control group to which no active ingredient is added first, it can be seen that the cells rapidly die after the irradiation of UV (+), as compared to before (-) irradiation of the ultraviolet ray. It can be seen that the killing of cells is prevented by C. In addition, in the case of Comparative Example 1 it can be seen that the results similar to the control 2.

On the other hand, in the case of the cell medium to which the fermented product according to the present invention is added, the cell activity is maintained at a certain level even after UV irradiation. It can be seen that the excellent degree of cellular activity is excellent. Thus, it can be seen that the fermentation of asyra according to the present invention is very excellent in protecting the skin from ultraviolet rays.

Evaluation example  2

Inhibition of inflammatory reactions caused by UV rays

First, human keratinocytes were added with DMEM (Dulbecco's Modified Eagle's Medium) medium containing penicillin (100 IU / mL), streptomycin (100 g / mL), and 10% FBS (fetal bovine serum). Culture was maintained in an incubator containing 5% carbon dioxide while maintaining the ℃. The obtained keratinocytes were dispensed into 6-well plates at 3 × 10 ⁵ cells per well, and then cultured for 24 hours under cell culture conditions. After culturing, the cells were irradiated with 25 mJ / Cm ² of ultraviolet rays, and the fermented products and extracts of Examples 1, 2 and Comparative Example were added to the medium at 0.004% and 0.008% of the total weight of the medium, respectively, and further cultured for 24 hours. . After culturing, the culture supernatant was recovered and the amount of inflammatory cytokine TNF-α was measured and shown in FIG. 2.

In addition, instead of adding the fermentation product according to the present invention, the fibroblast medium (control group 1) and the fermentation product according to the present invention, as a control, dexamethasone and WY-14643, which are generally used, are 1 μM and 200 μM, respectively. Prepared medium (control 2) was added to measure the secretion amount of inflammatory cytokine TNF-α according to the ultraviolet irradiation, shown in Figure 2 together.

Referring to FIG. 2, in the case of the control group 1, the secretion of TNF-α, which is an inflammatory cytokine after UV irradiation, is significantly increased, but in the case of the control group 2 to which the anti-inflammatory improving substance is added according to the conventional technique, the secretion amount is slightly decreased. Assay extract of Comparative Example also produces a similar level of reduction effect as in the prior art.

On the other hand, when the fermented product of Assy Berry is added according to the present invention, the secretion amount of TNF-α, an inflammatory cytokine, is significantly reduced, especially in Example 2 where fermented Assy Berry is fermented by a mixed strain. It is very large that it can be seen that an excellent improvement effect occurs compared to the conventionally known anti-inflammatory material. Therefore, it can be determined that the fermented ash berry according to the present invention has an excellent effect on prevention, improvement and treatment of cellular inflammation caused by UV irradiation.

Evaluation example  3

Inhibitory Effects of Melanin Synthesis on Melanin Tumor Cells

In order to determine the melanin synthesis inhibitory effect on the fermentation of assay berry of the present invention was carried out the following experiment.

First, the mouse-derived malignant melanin tumor cell line B16F1 (KCLB 8007) was added to DMEM (Dulbecco's Modified Eagle's Medium) medium containing penicillin (100 IU / mL), streptomycin (100 g / mL), and 10% FBS (fetal bovine serum). Incubated in an incubator containing 5% carbon dioxide while maintaining 37 ° C. The obtained melanin tumor cells were seeded in 1 × 10 ⁴ cells / well in a 24-well plate and cultured for 1 day. After incubation, the medium was removed, and in order to induce melanin synthesis, alpha-MSH (melanocyte stimulating hormone; melanocyte stimulating hormone) was added to the fermentations and extracts of Examples 1, 2 and Comparative Example, and incubated for 3 days. The weight percentages of the extracts and fermentations were 0.0004%, 0.004%, 0.02%, 0.04%. In addition, as a control, melanin tumor cells (UV (-)) which were not irradiated with ultraviolet rays, and melanin tumor cells to which MSH was added at 1 nM and 5 nM were prepared. In addition, the melanin tumor cells were cultured for 3 days. Then, the amount of synthesized melanin secreted by melanin tumor cells by each treatment group was visually checked and the change was photographed with a digital camera. The cells of each treatment group were collected to collect 10% DMSO (dimethyl sulfoxide) and 1N NaOH 100. After adding μl to dissolve melanin, the absorbance at 405 nm was measured, and the amount of melanin was measured. The results are shown in FIG. 3.

Referring to Figure 3, it can be seen that the assay fermented products of Examples 1 and 2 of the present invention reduces melanin production by UV irradiation in a concentration-dependent manner, whereby the assay fermented products according to the present invention are functional of a cosmetic for whitening. It turns out that it is very effective also as a component.

Evaluation example  4. Collagen synthesis promoting effect (anti-aging effect)

In order to measure the anti-aging effect of the assay fermentation of Examples 1 and 2, the effect of promoting collagen synthesis in human fibroblasts was examined.

First, normal human fibroblasts were charged with DMEM (Dulbecco's Modified Eagle's Medium) medium containing penicillin (100 IU / mL), streptomycin (100 g / mL), and 10% FBS (fetal bovine serum). Culture was maintained in an incubator containing 5% carbon dioxide while maintaining the ℃. The obtained fibroblasts were dispensed into 6-well plates at 3 × 10 ⁵ cells per well and incubated for 24 hours under cell culture conditions. After culturing, the cells were irradiated with 25mJ / Cm ² of ultraviolet rays, and the fermented products and extracts of Examples 1, 2 and Comparative Example were added at 0.0008%, 0.004%, 0.02%, and 0.04% by weight, respectively, and then cultured for 24 hours. . After the cells were recovered and crushed, the collagen production was measured after using a collagen collage kit (Sircol assay kit), which is shown in FIG. 4.

In addition, as a control, fibroblast medium without control of the assay fermentation according to the present invention (control 1) and fibroblast medium with addition of 25 μM ascorbic acid (vitamin C) instead of the assay fermentation according to the present invention (control 2). The amount of collagen produced for) was measured and shown in FIG. 4.

Referring to Figure 4, the cell medium added assay fermentation according to the present invention shows an excellent collagen production effect, in particular it can be seen that it is very superior to the vitamin C known to show excellent collagen production in the prior art. Furthermore, the concentration-dependent collagen production effect on assay fermentation shows that the assay fermentation product according to the present invention is excellent as an anti-aging component.

Evaluation example  5

Antioxidative effect

The antioxidant effect of the ash fermentation of Examples 1 and 2 was measured in this evaluation example.

To this end, first, diphenylpicrylhydrazyl (Diphenylpicrylhydrazyl, DPPH) is dissolved in ethanol to a concentration of 250 μM, and then the DPPH solution is diluted to prepare an absorbance at a wavelength of 517 nm in the range of 0.8 to 0.9. And 1: 1 and fermented with 2 and reacted for 20 minutes in the dark. After the reaction, the absorbance was measured at a wavelength of 517 nm to measure the antioxidant activity (Free radical scavenging activity) of the ash fermentation, which is shown in Table 1 and FIG. 5.

Referring to Table 1 and Figure 5, it can be seen that the fermented product of assay berry according to the present invention has a very excellent antioxidant power, showing an antioxidant activity increase of about 10% compared to the case without fermentation have.

Hereinafter, according to the following composition, formulations of soap, lotion, cream, pack and cosmetic liquid forms were prepared, respectively.

Production Example  One. Soap

Fermentation of Example 1 5.00

Upkeep 75

Sodium hydroxide 5

Spices 10

Purified water level

(Unit: wt%)

Production Example  2. Lotion Type

Fermentation of Example 1 3.00

L-ascorbic acid-2-magnesium phosphate salt 1.00

Water Soluble Collagen (1% Aqueous Solution) 1.00

Sodium Citrate 0.10

Citric Acid 0.05

Licorice Extract 0.20

1,3-butylene glycol 3.00

Purified water level

(Unit: wt%)

Production Example  3. Cream

Fermented product of Example 1 1.00

Polyethylene Glycol Monostearate 2.00

Self-emulsifying glycerin monostearate 5.00

Cetyl Alcohol 4.00

Squalene 6.00

Tri2-ethylhexaneglyceryl 6.00

Sphingolipids 1.00

1.3-butylene glycol 7.00

Purified water level

(Unit: wt%)

Production Example  4. Packed

Fermentation of Example 1 5.00

Polyvinyl Alcohol 13.00

L-ascorbic acid-2-magnesium phosphate salt 1.00

Lauroylhydroxyproline 1.00

Water Soluble Collagen (1% Aqueous Solution) 2.00

1,3-butylene glycol 3.00

Ethanol 5.00

Purified water level

(Unit: wt%)

Production Example  5. Cosmetic liquid type

Fermentation Example 2 2.00

Hydroxyethylene Cellulose (2% Aqueous Solution) 12.00

Xanthan gum (2% aqueous solution) 2.00

1,3-butylene glycol 6.00

Dark Glycerin 4.00

Sodium Hyaluronate (1% aqueous solution) 5.00

Purified water level

The above formulations represent one form using the Assy berry fermentation according to the present invention. The Assy berry fermentation according to the present invention may be used in various formulations used in the art, which is also within the scope of the present invention.

Ascai fermented product prepared according to the present invention has excellent efficacy of anti-aging, anticancer, anti-inflammatory, immunity, etc., and thus can be widely applied to various fields such as pharmaceutical industry, beauty industry and food industry.

1 is a graph showing the experimental results of Evaluation Example 1.

2 is a graph showing experimental results of Evaluation Example 2. FIG.

3 is a graph showing the experimental results of Evaluation Example 3.

4 is a graph showing the experimental results of Evaluation Example 4.

5 is a graph showing the experimental results of Evaluation Example 5.

Claims (9)

A cosmetic composition comprising a fermented product obtained by fermenting an extract of Ascai berry with a mixed strain of lactic acid bacteria and yeast as an active ingredient. delete The method of claim 1, Lactobacillus sakei ( Lactobacillus sakei B2-16 ), Lactobacillus brevis ( Lactobacillus brevis), Lactobacillus plantarum P23 , Lactococcus lactis ( Lactococcus lactis A164 ), Bacillus coagulance ( Bacillus coagulance ) Cosmetic composition, characterized in that at least one strain selected from the group consisting of. The method of claim 1, The cosmetic composition is a cosmetic composition, characterized in that the active ingredient is 0.05% to 95% by weight. The method according to any one of claims 1, 3 and 4, The cosmetic composition is a cosmetic composition, characterized in that one formulation selected from the group consisting of soap, lotion, cream, pack and essence. delete Obtaining an extract from asyra berry; Inoculating the mixed strains of lactic acid bacteria and yeast in the extract of the assay berry; And Fermentation method of the assay berry (Acai berry) comprising the step of culturing the inoculated assay berry extract to produce a fermentation product. The method of claim 7, wherein The inoculation step of the inoculation step is the inoculation amount of the inoculation berry fermentation method characterized in that the initial inoculation number of 10 ⁵ to 10 ⁸ cfu / g or 10 ⁵ to 10 ⁸ cfu / ㎖. The method of claim 7, wherein The fermentation method of the assay berry is characterized in that the step of producing the fermentation is carried out for 18 to 120 hours at a temperature of 15 to 40 ℃.

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