KR100898311B1 - Cosmetic composition containing extract of mixed fermentation with soy bean milk - Google Patents

Abstract

The present invention relates to a cosmetic containing an extract prepared from a fermentation broth obtained by complex culture of lactic acid bacteria and yeast, using soy milk as a substrate, and has excellent skin improvement effects such as cell regeneration, anti-wrinkle effect, whitening, and anti-inflammatory.

Combined fermentation, lactic acid bacteria, yeast, soy milk, cosmetics, external skin preparations

Description

Cosmetic composition containing soy milk complex fermented extract as an active ingredient {Cosmetic composition containing extract of mixed fermentation with soy bean milk}

The present invention relates to a cosmetic composition containing a complex culture extract of lactic acid bacteria and yeast as an active ingredient, more specifically, Leuconostoc mesenteroides , Lactobacillus kefiri , Cleveromyces The present invention relates to a cosmetic composition containing an extract obtained by filtering a fermentation product obtained by adding a complex strain consisting of ( Kluyveromyces ) to soy milk and culturing.

Soy milk contains active ingredients such as isoflavin and has been used for a long time since it is known to have skin improvement and lightening effects (Patent 10-0505322, Patent Application 10-2006-7009159).

Recently, as the development of foods and cosmetics using fermentation is increasing, interest in natural fermentation materials is increasing. Fermentation is a process in which organic matter is decomposed or synthesized by microorganisms and reproduced as essential nutrients for life. Techniques for using soymilk fermentation products in cosmetics include those using microorganisms of the genus Rhizopus (Japanese Patent Laid-Open No. Hei 1-10211) or those using lactic acid bacteria (Japanese Patent Laid-Open Hei 3-127715). In addition, an invention is disclosed for a soybean fermentation extract that is effective in improving rough skin and wrinkles by extracting a fermentation product obtained by applying a microorganism of Bifidobacterium to soybean extract with an organic solvent (Korea Patent 10- 10). 0577914). In addition, Korean Patent Laid-Open Publication No. 2003-61447 discloses an external preparation for skin containing soymilk fermentation product and vitamin A or a derivative thereof to prevent and improve roughness and dead skin of the skin.

The use of yeast fermented broth as cosmetics is very limited, and there are reports that Kombucha culture has an effect of delaying skin regeneration and skin aging (Patent 10-0466623). In addition, there is a report inventing a beverage having high physiological activity by culturing bifidus and yeast in glutinous rice flour and lactose (Patent 10-0561765).

In general, lactic acid bacteria are known to produce bioactive substances such as antimicrobial and lactic acid, and yeast is known to produce various vitamins as metabolites. In particular, strains of the genus Kluyveromyces in yeast have been reported to have the characteristics of helping to propagate lactic acid bacteria (Rada V. Folia Microbiol. 42, 145-148, 1997).

However, there has not yet been a case in which the complex culture solution obtained by using lactic acid bacteria and yeast and soymilk as a substrate or its extract was used as a cosmetic which has anti-wrinkle, whitening effect, cell regeneration, and anti-inflammatory effect.

An object of the present invention is to provide a cosmetic material containing a variety of physiologically active substances in addition to the ingredients in the soymilk itself in using the vegetable material and soy milk effective for skin improvement.

In order to achieve the above object, the present inventors have obtained a fermentation broth that exhibits better anti-aging, anti-inflammatory and whitening functions than the fermentation broth obtained by fermenting soymilk with yeast and lactic acid bacteria as a single microorganism.

The present invention provides a cosmetic composition containing an extract of the complex fermentation product obtained by adding lactic acid bacteria and yeast to culture soy milk as an active ingredient.

In addition, the present invention is one or more of the lactic acid bacteria is selected from the Leuconostoc mesenteroides ( Leuconostoc mesenteroides ) and Lactobacillus kefiri , yeast is a soy milk complex, characterized in that the genus Kluyveromyces ( Kluyveromyces ) It provides a cosmetic composition containing the extract of the fermented product as an active ingredient.

The present invention also provides a cosmetic composition containing the extract of the soymilk complex fermentation as an active ingredient, characterized in that the extract of the complex fermentation contains a polysaccharide.

In addition, the present invention provides a cosmetic composition containing the extract of the soymilk complex fermentation as an active ingredient, characterized in that the polysaccharide is contained in a concentration of 0.01% to 0.5%.

In addition, the present invention is the soymilk complex fermentation, characterized in that the cosmetic composition is one selected from a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type of formulation. Provided is a cosmetic composition containing an extract of water as an active ingredient.

According to the present invention, the complex fermentation extract of soy milk may be included as an active ingredient of a cosmetic composition by exhibiting excellent anti-aging, anti-inflammatory and whitening effect, and may be widely applied to various fields such as beauty industry, food industry, and pharmaceutical industry.

Soy milk used to achieve the object of the present invention (soy bean milk) comprises a soy milk whey solution or soy milk powder suspended in a constant concentration of more than 5% of the remaining solid after dipping and crushing soybeans.

The complex fermentation product defined in the present invention is added to the soy milk of at least one species of lactic acid bacteria consisting of Leuconostoc mesenteroides and Lactobacillus kefiri and yeast of the genus Kluyveromyces It means what is obtained by culturing.

In addition, the complex fermentation extract of the present invention is a composite fermentation product obtained by adding and culturing lactic acid bacteria and yeast in soy milk as described above or extracted by various extraction methods widely known to those skilled in the art to which the present invention belongs. it means.

The present invention provides a cosmetic composition containing a soy milk complex fermentation extract.

The present invention is characterized in that the content of the soymilk complex fermentation extract is 0.001 to 30.0% by weight based on the dry weight of the total cosmetic composition. When the content of the extract is less than 0.001% by weight, there is almost no skin improvement effect, and when the content of the extract is greater than 30.0% by weight, the effect of increasing the content is not economical.

The present invention provides a cosmetic composition containing the extract of the soymilk complex fermentation as an active ingredient, characterized in that the extract of the complex fermentation contains a polysaccharide.

The present invention provides a cosmetic composition containing the extract of the soymilk complex fermentation as an active ingredient, characterized in that the polysaccharide is contained at a concentration of 0.01% to 0.5% in the extract of the complex fermentation. When the polysaccharide content is less than 0.01% of the extract of the complex fermentation, the skin improvement effect is not remarkable. When the polysaccharide content exceeds 0.5% of the extract of the complex fermentation, the effect of increasing the content is not large.

In addition, the present invention is the soymilk complex fermentation extract, characterized in that the cosmetic composition is selected from a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type formulation It provides a cosmetic composition containing the main active ingredient.

The coating cosmetic containing the composition of the present invention as an active ingredient can be easily prepared in any form according to a conventional manufacturing method. As an example, in the preparation of a cream coating agent, the natural base composition of the present invention is contained in a general oil-in-water (O / W) or water-in-oil (W / O) cream base, and includes a fragrance, a chelating agent, a pigment, an antioxidant, and a preservative. Etc. may be used as necessary, and synthetic or natural materials such as proteins, minerals, vitamins, etc. may be used in combination for the purpose of improving the properties.

In addition, the cosmetic composition of the present invention can be formulated into a lotion, gel, water-soluble powder, fat-soluble powder, water-soluble liquid, cream or essence by adding a pH adjuster, flavoring, emulsifier, preservatives, etc. as needed, in a conventional cosmetic preparation method. Can be.

The configuration of the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the scope of the following examples.

Example 1 Preparation of Lactic Acid Bacteria and Yeast Composite Fermentation Products

Soybean powder 10% (w / v) was suspended in purified water and then sterilized for 30 minutes at 120 ℃. Lactobacillus kefiri R-1 (KCTC10725BP) consisting of Leuconostoc mesenteroides E-1 (KCTC10724BP) and Lactobacillus kefiri R-1 (KCTC10725BP) in sterile medium containing yeast of the genus Kluyveromyces The powder was inoculated with the seed. Each of the lactic acid bacteria and yeast was used at a concentration of 0.1% (W / V). The culture temperature was 25 ° C. and incubated for about 20 to 24 hours until the pH dropped to 4.5.

Table 1 shows changes in the media and culture components before and after fermentation. When soymilk was combined with the above method, the vitamin component was greatly increased and polysaccharides were produced compared to before the culture.

ingredient Pre-culture Content (mg / 100g) Content after cultivation (mg / 100g) Vitamin B1 0.33 0.88 Vitamin B6 0.02 0.05 Niacin 0.006 0.04 Polysaccharide 0.0 13.4

[Comparative Examples 1 and 2]

In the same conditions as in Example 1, except that the lactic acid bacteria were inoculated with the two kinds of lactic acid bacteria and yeast without complex culture, Comparative Example 1, and inoculated with the yeast was carried out as Comparative Example 2.

Example 2 Preparation of Extract from Composite Fermentation

The combined fermentation product obtained in Example 1 was separated by supernatant at 8000 rpm in a continuous centrifuge. A membrane filtrator equipped with a 0.2 μm filter was used to remove the cells from the supernatant. After the addition of the appropriate preservatives were stored for 1 day at 4 ~ 10 ℃. The solid was repeatedly removed using a membrane filter to obtain a clear composite fermentation extract.

Evaluation Example 1 Effect of alleviating skin cytotoxicity and growth promoting effect by ultraviolet ray

Normal human fibroblasts were placed in Dulbecco's Modified Eagle's Medium (DMEM) medium containing penicillin (100 IU / ml), streptomycin (100 g / ml), and 10% fetal bovine serum (FBS) and maintained at 37 ° C. And cultured in an incubator containing 5% carbon dioxide. Fibroblasts obtained after the culture were dispensed into 3 × 10 ⁵ cells per well in 6-well plates, and then cultured for 24 hours under cell culture conditions. After culturing, the cells were irradiated with 25 mJ / cm 2 of ultraviolet rays, and further cultured for 24 hours after the addition of the complex fermentation extract obtained in Example 2 and each control. Cell number was measured to determine the degree of cell death and growth promotion.

As shown in Figure 1 it can be seen that the use of the complex fermentation extract is superior to the control and soy milk supernatant, and the cell growth promoting effect compared to the group using yeast or lactic acid bacteria alone.

Evaluation Example 2 Inhibition of Inflammatory Response Induced by UV Light

Human keratinocytes (ATCC CRL-2404) were placed in DMEM (Dulbecco's Modified Eagle's Medium) medium containing penicillin (100 IU / mL), streptomycin (100 g / mL) and 10% FBS (fetal bovine serum). Culture was maintained in an incubator containing 5% carbon dioxide while maintaining 37 ℃. The obtained keratinocytes were dispensed into 6-well plates at 3 X 10 ⁵ per well and incubated for 24 hours under cell culture conditions. After culturing, the cells were irradiated with 25mJ / cm 2 of ultraviolet rays, and further cultured for 24 hours after the addition of the combined fermentation extract. The culture supernatant was recovered and the amount of inflammatory cytokine TNF-α was measured and shown in FIG. 2.

In the case of the combined fermentation extract, it can be seen that an excellent anti-inflammatory effect is suppressed by suppressing the increase of TNF-α secretion by stimulation of ultraviolet rays compared to the group using the soymilk supernatant or yeast or lactic acid bacteria alone. In addition, it can be seen that it shows similar efficacy to dexamethasone (dexamethasone) which is widely used as an anti-inflammatory active ingredient.

Evaluation Example 3 Collagen Synthesis Promoting Effect (Anti-Aging Effect)

In order to measure the anti-aging effect of the combined fermentation extract obtained in Example 2, the effect of promoting collagen synthesis in human fibroblasts was examined.

Normal human fibroblasts (ATCC CCL110) were placed in DMEM (Dulbecco's Modified Eagle's Medium) medium containing penicillin (100 IU / mL), streptomycin (100 g / mL) and 10% FBS (fetal bovine serum). Were incubated in an incubator containing 5% carbon dioxide. The obtained fibroblasts were dispensed into 6-well plates at 3 X 10 ⁵ per well and incubated for 24 hours under cell culture conditions.

After culturing, the cells were irradiated with 25 mJ / cm 2 of ultraviolet rays, and the cultures were incubated for 24 hours after the addition of the composite fermentation extract of Example 2. After the cells were recovered and crushed, the amount of collagen was measured using a Circol collagen assay kit.

As shown in FIG. 3, the soymilk complex fermentation extract showed an excellent effect of inhibiting the breakdown of collagen by stimulation of ultraviolet rays and promoting the synthesis of collagen compared to the group treated with soymilk or yeast fermentation extract and lactic acid bacteria fermentation extract alone. Can be.

Evaluation Example 4 Inhibitory Effect of Melanin Synthesis in Melanin Tumor Cells

In order to determine the melanin synthesis inhibitory effect of the composite fermentation extract obtained in Example 2, the following experiment was performed.

First, the mouse-derived malignant melanocyte tumor cell line B16F1 (KCLB 8007) was put into DMEM (Dulbecco's Modified Eagle's Medium) medium containing penicillin (100 IU / mL), streptomycin (100 g / mL), and 10% FBS (fetal bovine serum). Culture was maintained in an incubator containing 5% carbon dioxide while maintaining the ℃. The obtained melanin tumor cells were inoculated in 1 × 10 ⁴ cells / well in 24 well plates and cultured for one day. After incubation, the medium was removed, and in order to induce melanin synthesis, melanin synthesis was induced by adding 5 nM of α-MSH (melanocyte stimulating hormone) except for the non-treated group. (Positive control) DMEM medium to which 800 μM was added was added and cultured for 3 days. The amount of synthesized melanin secreted by melanin tumor cells of each treatment group was visually checked and the changes were photographed with a digital camera. The cells of each treatment group were collected, and 100 μl of 10% DMSO (dimethyl sulfoxide) and 1N NaOH, respectively. Melanin was dissolved by addition, and the melanin amount was measured by measuring the absorbance at 405 nm.

As shown in Figure 4 it can be seen that the composite fermentation extract of Example 2 reduces melanin production by UV irradiation in a concentration-dependent manner.

Soap-type, lotion-type, cream-type, pack-type, and essence-type preparations were prepared according to the following compositions using the composite fermentation extract prepared in Example 2.

Example 3 Soap Formulation

Combined Fermentation Extract of Example 2 5.00

Upkeep 75

Sodium hydroxide 5

Spices 10

Purified water level

(Unit: weight%)

Example 4 Lotion Formulation

Extract 3.00 of Example 2

L-ascorbic acid-2-magnesium phosphate salt 1.00

Water Soluble Collagen (1% Aqueous Solution) 1.00

Sodium Citrate 0.10

Citric Acid 0.05

Licorice Extract 0.20

1,3-butylene glycol 3.00

Purified water level

(Unit: weight%)

Example 5 Cream Formulation

Extract 1.00 of Example 2

Polyethylene Glycol Monostearate 2.00

Self-emulsifying glycerin monostearate 5.00

Cetyl Alcohol 4.00

Squalene 6.00

Tri2-ethylhexaneglyceryl 6.00

Sphingolipids 1.00

1.3-butylene glycol 7.00

Purified water level

(Unit: weight%)

Example 6 Packed Formulations

Extract of Example 2 5.00

Polyvinyl Alcohol 13.00

L-ascorbic acid-2-magnesium phosphate salt 1.00

Lauroylhydroxyproline 1.00

Water Soluble Collagen (1% Aqueous Solution) 2.00

1,3-butylene glycol 3.00

Ethanol 5.00

Purified water level

(Unit: weight%)

Example 7 Cosmetic Liquid Formulation

Extract 2.00 of Example 2

Hydroxyethylene Cellulose (2% Aqueous Solution) 12.00

Xanthan gum (2% aqueous solution) 2.00

1,3-butylene glycol 6.00

Dark Glycerin 4.00

Sodium Hyaluronate (1% aqueous solution) 5.00

Purified water level

(Unit: weight%)

Figure 1 is a graph showing the results of comparing the effects of soy milk and soy milk complex fermentation extract on the growth and toxicity of cells.

Figure 2 is a graph showing the results of comparing the effects of inhibiting the production of TNF-α anti-inflammatory factors soy milk and soy milk complex fermentation extract.

Figure 3 is a graph showing the results of measuring the effect of the soy milk and soy milk complex fermentation extract on the change in collagen amount after irradiation with ultraviolet rays. On the left is the collagen content (%) compared to the control.

Figure 4 is a graph showing the results of measuring the amount of melanin biosynthesis according to the concentration of the soymilk complex fermentation extract. On the left is the 405 nm absorbance (%) compared to the control. KA stands for Kojiic Acid.

Claims (5)

Compound fermentation obtained by adding and cultivating soymilk to at least one lactic acid bacterium selected from Leuconostoc mesenteroides E-1 and Lactobacillus kefiri R-1 and yeast of Kluyveromyces A whitening functional cosmetic composition containing an extract of water as an active ingredient, and containing polysaccharide at a concentration of 0.01% to 0.5%. delete delete delete delete

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