KR20090113723A - A cosmetic composition, a pharmaceutical composition comprising extract or fermented product from labisia pumila - Google Patents

Abstract

PURPOSE: A cosmetic composition and a pharmaceutical composition containing extract or fermentation product of Labisia pumila are provided to ensure high activity of UV protection, whitening, anti-aging and anti-oxidation and improve skin function. CONSTITUTION: A cosmetic composition contains Labisia pumila extract, fermentation of Labisia pumila extract or their mixture as an active ingredient. The Labisia pumila extract is isolated using an organic solvent of ethanol, hexane, or acetone. The cosmetic composition is a cosmetic composition for skin moisturization, anti-aging, whitening, anti-inflammtion or UV protection. The cosmetic composition is used in the form of soap, lotion, cream, pack and cosmetic liquid.

Description

Cosmetic composition, a pharmaceutical composition comprising extract or fermented product from labisia pumila}

The present invention relates to a cosmetic composition, pharmaceutical composition comprising an extract or fermentation of Labisia pumila, and more particularly to a cosmetic composition and ultraviolet light having excellent UV blocking, whitening, anti-aging and antioxidant effects. It relates to a pharmaceutical composition excellent in the treatment effect of skin inflammation caused by.

With the recent advent of an aging society and the spread of well-being trends, there is an increasing demand for maintaining health and beauty for a long time. Interest in materials is increasing.

Natural plant materials have been used for a long time in the cosmetics industry because they are safer and more environmentally friendly than synthetic materials. Recently, herbal cosmetics using herbal medicine with strong physiological activity has become the mainstream of cosmetic products. Herbal cosmetics have been used for hundreds of years and have been proven in terms of safety and effectiveness, so they can be easily recognized and used by consumers, and the effect is inferior to synthetic materials.

Natural plant material or herbal medicine contains various biologically active substances and antioxidants and has various effects such as anti-aging, anti-cancer, anti-inflammatory, and immune function improvement.

There are many substances that have excellent physiological activity on the skin and have anti-aging and whitening effects. Retinol, arbutin and vitamin C are known to be effective for wrinkle improvement and whitening as a representative functional material. However, most of these attempts to find a substitute material by instability, ineffectiveness or side effects in the formulation are an essential part of cosmetic material research. Recently, attempts have been made to search for substances with excellent physiological activity in natural plant materials, not synthetic ones, and plants and herbal medicines that have useful effects on skin, such as anti-aging, anti-inflammatory, and whitening, are found one after another. .

In particular, the Applicant noticed a plant called Labisia pumila, which belongs to the Myrsinaceae family, which is also called Kacip fatimah, which grows only in Malaysia and Kalimantan Peninsula. . Laviia pumila has been traditionally used in Malaysia to be called the Queen of Herbs, and is known for its high biological activity.

In particular, research from the Malaysian Herbal Research Center has shown that labia increases free testosterone levels and does not increase estrogen. The study reported that Ravizia could act as an estrogen receptor modulator (SERMS), such as tamoxyfan or raloxifene, and showed activity in certain tissue cells. Further, a 1992 study demonstrated that Ravizia exhibited estrogen properties, and in experiments with endometrial adenocarcinoma cells, a mild but peculiar estrogen effect was found to enhance alkaline organophosphate secretion in living cells from ethanol extracts of Ravigia. In a 2003 study, Lavizia water soluble extracts reportedly enabled estridiol binding transfer to enhance antibodies against estradiol, producing something similar to other estrogen substances such as estrone and estradiol.

However, the above-described techniques and studies using Ravizi fumila are all related to estrogen, a female hormone, and studies on other effects and application thereof are insufficient.

Therefore, the first problem to be solved by the present invention is to provide a cosmetic composition comprising the extract of Raviggia fumila or fermented product as an active ingredient.

The second problem to be solved by the present invention is to provide a pharmaceutical composition comprising the extract of Raviggia fumila or fermented product as an active ingredient.

In order to solve the first problem, the present invention is an active ingredient of at least one selected from the group consisting of the extract of Labia pumila (Labisia pumila), the fermentation of the extract of Lavizi pumila and mixtures thereof It provides a cosmetic composition. In one embodiment of the present invention, the Lavizi fumila extract is a water-soluble extract, in another embodiment the Lavizi fumila extract is an oil-soluble extract.

In addition, in one embodiment of the present invention, the active ingredient is 0.001 to 95% by weight based on the total weight of the composition, the cosmetic composition comprising the Ravigia fumila extract or fermented product as an active ingredient moisturizing skin, anti-aging, Cosmetic composition for whitening, anti-inflammatory or sun protection. In addition, the cosmetic composition may take one formulation selected from the group consisting of soaps, lotions, creams, packs and essences.

In order to solve the second problem, the present invention is an active ingredient of at least one selected from the group consisting of extracts of Labisia pumila, fermented products of the extract of Lavizia pumila and mixtures thereof It provides a pharmaceutical composition for skin treatment.

The cosmetic composition of the present invention using the extract or fermented product of Ravigia pumila or a mixture thereof as an active ingredient has excellent UV blocking, whitening, anti-aging and antioxidant effects, and thus is particularly a functional ingredient of cosmetics for improving skin function. It is suitable, and the effect is also excellent. Furthermore, since the composition of the extract or fermented product of Ravigia fumila or a mixture thereof as an active ingredient shows an excellent effect in treating inflammation caused by ultraviolet rays, it is also excellent as a therapeutic agent for skin inflammation caused by excessive UV exposure. Do.

Hereinafter, the present invention will be described in more detail with reference to the drawings and examples. However, the present invention is not limited by the following drawings and examples.

As described above, the present invention provides a cosmetic composition comprising as an active ingredient at least one selected from the group consisting of extracts of Ravizi fumila, fermented products of the extracts, and mixtures thereof.

Herein, the extract may be a water-soluble extract extracted with a water-soluble solvent such as water or an oil-soluble extract extracted from an organic solvent such as ethanol, hexane, acetone, or any method as long as an effective extract can be obtained from Raviggia pumila. It can be used in the scope of the present invention. Therefore, it is possible to freely select the extract form of Lavizi fumila according to the characteristics of the formulation to be used. Furthermore, the present invention provides a cosmetic composition comprising the fermented product obtained by fermenting the extract obtained from the Ravigia pumila as an active ingredient. In general, fermentation is a process of preparing a substance useful for human life by decomposing organic matter using an enzyme possessed by microorganisms. Particularly, fermentation product of Ravizi fumila, which fermented the extract of Ravizi fumila with lactic acid bacteria or yeast, It shows a very good effect on reducing the toxicity of skin cells, which will be described in more detail in the following evaluation example.

In one embodiment of the present invention, the Lavizi fumila extract or fermented product or mixtures thereof is preferably 0.001 to 95% by weight relative to the total weight of the composition. If less than the above range Ravizi fumila extract or fermentation is ineffective, in many cases uneconomical, it is difficult to expect a variety of effects depending on the other composition ingredients added to the composition. That is, the cosmetic composition according to the present invention may contain other ingredients and the like which can give a synergistic effect to the main effect within the range that does not impair the main effect by the active ingredient, for example, fragrances, In addition to additives such as pigments, fungicides, antioxidants, preservatives, humectants, thickeners, inorganic salts, emulsifiers, synthetic polymers, and the like, as well as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides and seaweed extracts It may further comprise an auxiliary component. The components may be appropriately selected and blended by those skilled in the art according to the formulation or purpose of use, and the addition amount may be selected within a range that does not impair the object and effect of the present invention, but 0.01 to 5 weight based on the total weight of the composition. %, Preferably 0.01 to 3% by weight. Therefore, if the content of the active ingredient is more than 95% by weight, it is difficult to add such a functional ingredient.

In addition, the cosmetic composition comprising a labia pumila extract or fermented product or a mixture thereof as an active ingredient is a cosmetic composition that can be used for the purpose of moisturizing skin, anti-aging, whitening, anti-inflammatory or sun protection. That is, the present invention relates to a cosmetic composition using a novel skin effect improvement effect of Ravigian pumila, not the effect of improving the female hormone of the known Ravigia pumila, and the effect of improving the skin effect of Ravigia pumila is as follows. It will be described in more detail through an evaluation example.

The cosmetic composition according to the present invention may be one formulation selected from the group consisting of soaps, lotions, creams, packs, and cosmetic liquids. That is, the composition according to the present invention may be included in soap, lotion, cream, pack and cosmetic liquid, which is preferably in the form of an external skin preparation, and may be applied to the skin. However, this is only one example, and cosmetics of any formulation including the cosmetic composition according to the present invention are within the scope of the present invention, and the present invention is not limited to the types of the formulations listed above. For example, it may be administered in various forms such as a solution, an emulsion, a viscous mixture, a tablet, a powder, or the like, in a variety of ways such as simple drinking, injection, spray or squeeze.

In order to solve the second problem, the present invention provides a composition for the treatment of skin as an active ingredient extract or fermentation of Lavizi fumila or a mixture thereof. Since the composition for treating skin according to the present invention is particularly excellent for treating skin inflammation caused by excessive irradiation of ultraviolet rays, it can be usefully used to treat various skin diseases and skin inflammation caused by excessive tanning.

Hereinafter, the present invention will be described in more detail with reference to Examples and Evaluation Examples. However, these examples and evaluation examples are intended to explain the present invention in more detail, and the scope of the present invention is not limited by these examples and evaluation examples according to the gist of the present invention is common knowledge in the art. It will be self-evident for those who have.

Example  One

According to the invention Ravigia  Preparation of Water Soluble Extract

Wash 100g Lavizi fumila with cold water below 30 ℃ for about 30 minutes, grind it with a grinder, and add water so that the powder is 10% by weight based on solids, and then, in the range of 25 ℃ -100 ℃, To extract for 5 hours, filtered and dried.

Example  2

According to the invention Ravigia  Preparation of Oil-Soluble Extracts

30 g of Ravigia fumila was washed with cold water of 30 ° C. or less for about 30 minutes and pulverized with a pulverizer. The ratio of the pulverized product and the solvent was 1: 4. . Nucleic acid, ethanol, acetone were used as the solvent used for the oil-soluble extract.

Example  3

According to the invention Ravigia Fermented product  Produce

The water-soluble extract obtained in Example 1 was dissolved in water so as to be 3% based on solid content, and then sterilized by filtering with a 0.2 mm filter. Inoculated with lactic acid bacteria and yeast in the sterilized water-soluble extract so that the initial number of bacteria was 10 ⁵ to 10 ⁸ cfu / g, and then cultured for 3 days at 30 ℃ and centrifuged to obtain a fermentation supernatant.

Evaluation example  One

By ultraviolet Skin cytotoxicity  Reduction effect

First, normal human fibroblasts were charged with DMEM (Dulbecco's Modified Eagle's Medium) medium containing penicillin (100 IU / mL), streptomycin (100 g / mL), and 10% FBS (fetal bovine serum). Culture was maintained in an incubator containing 5% carbon dioxide while maintaining the ℃. The obtained fibroblasts were dispensed into 6-well plates at 3 × 10 ⁵ cells per well and incubated for 24 hours under cell culture conditions. After culturing, the cells were irradiated with 25mJ / Cm ² of ultraviolet rays, and then the labia water-soluble extract, oil-soluble extract, and fermented product were added at 0.00005%, 0.0001%, 0.0005%, 0.001%, and 0.005% of the total weight of the medium, respectively. After incubation for a time, ultraviolet (312nm, 25, J / cm 2) was irradiated to the medium and then measured the number of cells to measure the degree of cell death is shown in FIG.

In addition, ascorbic acid (vitamin C) was added at 12.5 μM and 25 μM instead of the fibroblast medium (control group 1) without addition of the extract or the fermentation product according to the present invention as a control and the extract or the fermentation product according to the present invention. One fibroblast medium (control group 2) was prepared to measure the degree of bacterial death according to ultraviolet irradiation, which is shown in FIG.

Referring to FIG. 1, in the case of the control group to which no active ingredient is added, about 50% of the cells are killed when irradiated with ultraviolet rays. In FIG. 1, the number of cells in the state not irradiated with ultraviolet rays is 100% and the state in which cells are killed about 50% by irradiation with ultraviolet rays is marked as 0% to determine how much cell death is prevented by adding an example material. .

On the other hand, in the case of the cell medium to which the extract or fermented product according to the present invention is added, the cell activity is maintained at a certain level even after UV irradiation, and in particular, the fermented product is superior to the control group 2 to which vitamin C is added according to the prior art. It can be seen that the cell activity degree (90% or more). As a result, it can be seen that the Ravizi fumila extract or fermented product according to the present invention is very excellent in protecting the skin from ultraviolet rays.

Evaluation example  2

Inhibition of inflammatory reactions caused by UV rays

First, human keratinocytes were added with DMEM (Dulbecco's Modified Eagle's Medium) medium containing penicillin (100 IU / mL), streptomycin (100 g / mL), and 10% FBS (fetal bovine serum). Culture was maintained in an incubator containing 5% carbon dioxide while maintaining the ℃. The obtained keratinocytes were dispensed into 6-well plates at 3 × 10 ⁵ cells per well and incubated for 24 hours under cell culture conditions. After culturing, the cells were irradiated with 25mJ / Cm ² of ultraviolet ray, and then the labia water-soluble extract, oil-soluble extract, and fermented product were added to the medium at 0.001%, 0.004%, 0.007%, and 0.01% of the total weight of the medium, respectively. Time incubated further. After culturing, the culture supernatant was recovered, and the secretion amount of inflammatory cytokines TNF-α and IL-1a was measured and shown in FIGS. 2A and 2B, respectively.

In addition, ascorbic acid (vitamin C) was added at 12.5 μM and 25 μM instead of the fibroblast medium (control group 1) without addition of the extract or the fermentation product according to the present invention as a control and the extract or the fermentation product according to the present invention. One fibroblast medium (Control 2) was prepared to measure the secretion amount of inflammatory cytokines TNF-α and IL-1a in response to ultraviolet irradiation, which is shown together in Figures 2a and 2b.

2A and 2B, the amount of secretion of the inflammatory cytokines TNF-α and IL-1α after UV irradiation in the control group 1 was significantly increased to 16pg / ml for TNF-α and 13pg for IL-1α. Although close to / ml, it can be seen that the amount of secretion decreases when vitamin C is added according to the conventional technique.

On the other hand, when the extract or fermented product of Ravizi pumila according to the present invention, the secretion amount of inflammatory cytokines TNF-α and IL-1α is significantly reduced, especially in the case of water-soluble extract or fermented product, the amount of inflammatory cytokine secretion is reduced. It can be seen from the above experimental results that is very large. Therefore, it can be judged that the extract or fermented product of Ravizi pumila according to the present invention has an excellent effect on prevention, improvement and treatment of cellular inflammation caused by UV irradiation.

Evaluation example  3

Inhibitory Effects of Melanin Synthesis on Melanin Tumor Cells

In order to determine the melanin synthesis inhibitory effect in the melanin tumor cells of the Ravigia extract and fermentation according to Examples 1 to 3 was performed as follows.

First, the mouse-derived malignant melanin tumor cell line B16F1 (KCLB 8007) was added to DMEM (Dulbecco's Modified Eagle's Medium) medium containing penicillin (100 IU / mL), streptomycin (100 g / mL), and 10% FBS (fetal bovine serum). Incubated in an incubator containing 5% carbon dioxide while maintaining 37 ° C. The obtained melanin tumor cells were seeded in 1 × 10 ⁴ cells / well in a 24-well plate and cultured for 1 day. After incubation, the medium was removed, and then, in order to induce melanin synthesis, alpha-MSH (melanocyte stimulating hormone; melanocyte stimulating hormone) was added to the labiasia water-soluble extract, oil-soluble extract, and fermentation of Examples 1 to 3, followed by 3 days of culture. At this time, the weight percentage of each Ravigia water-soluble extract, oil-soluble extract, fermentation was 0.0001%, 0.001%, 0.005%, 0.01%. In addition, as a control, melanin tumor cells (UV (-)) which were not irradiated with ultraviolet rays, and melanin tumor cells to which MSH was added at 1 nM and 5 nM were prepared, and DMEM medium to which 100 μM and 800 μM of kojic acid (KA), which was added as a positive control group, was added, In addition, the melanin tumor cells were cultured for 3 days. Then, the amount of synthesized melanin secreted by melanin tumor cells by each treatment group was visually checked and the change was photographed with a digital camera. The cells of each treatment group were collected to collect 10% DMSO (dimethyl sulfoxide) and 1N NaOH 100. After adding μl to dissolve melanin, the absorbance at 405 nm was measured, and the amount of melanin was measured. The results are shown in FIG. 3.

Referring to Figure 3, it can be seen that the extract or fermented product of Examples 1 to 3 of the present invention reduces melanin production by UV irradiation in a concentration-dependent manner, whereby the extract or fermented product according to the present invention is a cosmetic for whitening. It turns out that it is very effective also as a functional component of.

Evaluation example  4. Collagen synthesis promoting effect (anti-aging effect)

In order to measure the anti-aging effect of the Ravigia extract and fermented products according to Examples 1 to 3 was examined the effect of promoting collagen synthesis in human fibroblasts.

First, normal human fibroblasts were charged with DMEM (Dulbecco's Modified Eagle's Medium) medium containing penicillin (100 IU / mL), streptomycin (100 g / mL), and 10% FBS (fetal bovine serum). Culture was maintained in an incubator containing 5% carbon dioxide while maintaining the ℃. The obtained fibroblasts were dispensed into 6-well plates at 3 × 10 ⁵ cells per well and incubated for 24 hours under cell culture conditions. After culturing, the cells were irradiated with 25 mJ / Cm ² of ultraviolet rays, and then added with Ravizia water-soluble extract, oil-soluble extract, and fermented products at 0.00005%, 0.0001%, 0.0005%, 0.001%, and 0.005% by weight, respectively, for 24 hours. Incubated. After the cells were recovered and crushed, the collagen production was measured after using a collagen collage kit (Sircol assay kit), which is shown in FIG. 4.

Also, 12.5 μM and 25 μM of ascorbic acid (vitamin C) were added instead of the fibroblast medium (control group 1) without addition of the extract or the fermentation product according to the present invention as a control and the extract or the fermentation product according to the present invention. Collagen production amount for one fibroblast medium (Control 2) was measured, which is shown in FIG.

Referring to Figure 4, the cell medium added with the extract or fermentation of the Lazivia pumila according to the present invention shows an excellent collagen production effect, especially compared to vitamin C known to show excellent collagen production effect in the prior art It can be seen that it is excellent, furthermore, the concentration-dependent collagen production effect on the Lazivia pumila extract or fermentation shows that the Lazivia pumila extract or fermentation according to the present invention is excellent as an anti-aging component.

Evaluation example  5

Antioxidative effect

Antioxidant effect of the Ravigia extract and fermentation according to Examples 1 to 3 was measured in this evaluation example.

To this end, first dissolve diphenylpicrylhydrazyl (Diphenylpicrylhydrazyl, DPPH) in ethanol to a concentration of 250μM, and then dilute the DPPH solution to prepare an absorbance at the wavelength of 517nm in the range of 0.8 ~ 0.9 and then water soluble in labia Mix with extract, oil-soluble extract, fermented 1: 1 and react in the dark for 20 minutes. After the reaction, the absorbance was measured at a wavelength of 517 nm to measure the antioxidant activity (Free radical scavenging activity) of the Ravigia extract sample, which is shown in Table 1 below.

Referring to Table 1, it can be seen that the Ravigia extract and the fermented product according to the present invention have excellent antioxidant power.

Hereinafter, according to the following composition, formulations of soap, lotion, cream, pack and cosmetic liquid forms were prepared, respectively.

Production Example  One

Soap

Extract of Example 1 5.00

Upkeep 75

Sodium hydroxide 5

Spices 10

Purified water level

(Unit: weight%)

Production Example  2

Lotion type

Extract of Example 1 3.00

L-ascorbic acid-2-magnesium phosphate salt 1.00

Water Soluble Collagen (1% Aqueous Solution) 1.00

Sodium Citrate 0.10

Citric Acid 0.05

Licorice Extract 0.20

1,3-butylene glycol 3.00

Purified water level

(Unit: weight%)

Production Example  3

Cream

Extract of Example 1 1.00

Polyethylene Glycol Monostearate 2.00

Self-emulsifying glycerin monostearate 5.00

Cetyl Alcohol 4.00

Squalene 6.00

Tri2-ethylhexaneglyceryl 6.00

Sphingolipids 1.00

1.3-butylene glycol 7.00

Purified water level

(Unit: weight%)

Production Example  4

Packed

Extract of Example 1 5.00

Polyvinyl Alcohol 13.00

L-ascorbic acid-2-magnesium phosphate salt 1.00

Lauroylhydroxyproline 1.00

Water Soluble Collagen (1% Aqueous Solution) 2.00

1,3-butylene glycol 3.00

Ethanol 5.00

Purified water level

(Unit: weight%)

Production Example  5

Cosmetic liquid type

Extract 2.00 of Example 1

Hydroxyethylene Cellulose (2% Aqueous Solution) 12.00

Xanthan gum (2% aqueous solution) 2.00

1,3-butylene glycol 6.00

Dark Glycerin 4.00

Sodium Hyaluronate (1% aqueous solution) 5.00

Purified water level

The above formulations represent one form using the Ravizi fumila extract or fermentation product according to the present invention, the Ravizi fumila extract or fermentation product according to the present invention can be used in various formulations used in the art, and It belongs to the scope of the present invention.

Labisia pumila (Kacip fatimah) extract and fermented product prepared according to the present invention has excellent anti-aging, anti-cancer, anti-inflammatory, immunity improvement, etc., so that various fields such as pharmaceutical industry, beauty industry and food industry Widely applicable to

1 is a graph showing the experimental results of Evaluation Example 1.

2A and 2B are graphs showing experimental results of Evaluation Example 2. FIG.

3 is a graph showing the experimental results of Evaluation Example 3.

4 is a graph showing the experimental results of Evaluation Example 4.

Claims (7)

A cosmetic composition comprising at least one selected from the group consisting of extracts of labia pumila, fermented products of the extract of labia pumila, and mixtures thereof. The method of claim 1, The Lavizi pumila extract is a cosmetic composition, characterized in that the water-soluble extract. The method of claim 1, The Lavizi pumila extract is a cosmetic composition, characterized in that the oil-soluble extract. The method of claim 1, The active ingredient is a cosmetic composition, characterized in that 0.001 to 95% by weight based on the total composition. The method of claim 1, The cosmetic composition is a cosmetic composition, characterized in that the skin moisturizing, anti-aging, whitening, anti-inflammatory or sunscreen cosmetic composition. The method according to claim 1, wherein The cosmetic composition is a cosmetic composition, characterized in that one formulation selected from the group consisting of soap, lotion, cream, pack and essence. A pharmaceutical composition for treating skin, comprising at least one selected from the group consisting of extracts of Lavizi pumila, fermented products of the extract of Lavizi pumila, and mixtures thereof.

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