KR20230049911A - Cosmetic composition comprising probiotics culture - Google Patents

Abstract

The present invention relates to a cosmetic composition containing, as active ingredients, a culture containing a medium and lactic acid bacteria cells after culturing lactic acid bacteria, and a plant extract comprising one or more extracts selected from new green leaves, red mustard leaves, broccoli leaves, green mustard leaves, and broccoli flower stalks/buds, wherein the cosmetic composition of the present invention inhibits the production of an MMP-1 protein, promotes collagen production, and has the ability to inhibit tyrosinase and melanin production, thereby having excellent skin whitening and wrinkle alleviating effects.

Description

Cosmetic composition containing probiotics culture {COSMETIC COMPOSITION COMPRISING PROBIOTICS CULTURE}

The present invention relates to a cosmetic composition containing a probiotics culture, and more particularly, to a cosmetic composition for whitening or wrinkle improvement containing a probiotics culture and a plant extract as active ingredients.

The skin is the largest organ in the body and always directly in contact with the external environment, playing the role of a protective film to protect the living body from various stimuli or dry environments. In addition, it protects the body from physical abrasions, prevents loss of moisture inside the body, protects from ultraviolet rays generated by the sun, and plays a very important role in regulating the body temperature.

Such skin causes problems such as skin contamination, dryness, trouble, skin immune system abnormality, etc. due to various physical factors, external environmental factors, infections, and the like. In particular, with the rapid industrial development of modern times, pollution problems such as air pollution and water pollution have become serious, and the strong cooling and heating of the living space due to the improvement of the residential environment causes the skin to become rough, dry, and aging. are greatly influenced by Human skin gradually becomes thinner due to intrinsic aging and photoaging, wrinkles increase, elasticity decreases, and spots and freckles increase due to exposure to ultraviolet rays. In particular, endogenous aging occurs when the secretion of hormones decreases or the function and activity of immune cells decrease, resulting in a decrease in the biosynthesis of biocomponent proteins, which progresses rapidly with an increase in active oxygen.

It is known that humans constantly generate reactive oxygen species in the metabolic process, which act as direct or indirect causes of aging. In order to prevent skin aging, various antioxidants are used in cosmetics, and typical synthetic antioxidants include butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), and tertiary butylhydroquinone (TBHQ). In general, synthetic antioxidants are widely used due to their excellent effects and low prices, but their use is limited because they are known to cause liver enlargement, fatty mutations, and cancer as well as self-toxicity when in oral or skin contact.

Niacinamide and arbutin, which are used as raw materials for whitening functional cosmetics, and retinol, which are used as raw materials for wrinkle-improving functional cosmetics, have a problem in that the whitening effect is reduced due to the destruction of ingredients by oxidation and the restriction of the mixing amount due to skin irritation. Recently, as various cosmetic compositions using natural materials have appeared on the market, consumers have become interested in products using natural materials rather than products made of existing chemical materials, and accordingly, cosmetic compositions using various natural materials are being manufactured.

In addition, the anti-wrinkle effect of cosmetics is based on the effect of improving the dermis layer by various active ingredients in addition to the temporary effect of moisturizing by moisturizing factors, and the growth of retinol, vitamin C, and EGF, which are representative raw materials of wrinkle-improving cosmetics, Factor components are well known to exhibit anti-wrinkle effects by increasing collagen generation from human dermal fibroblasts. In order to prevent this phenomenon and maintain a healthier and more beautiful skin, physiologically active substances obtained from various known animals, plants, microorganisms, etc. are added to cosmetics and used to maintain the unique function of the skin and activate skin cells to Efforts have been made to effectively inhibit aging.

In the field of cosmetics, research and development on functional cosmetics having whitening and wrinkle-improving effects are being actively conducted, and in particular, studies using natural substances are continuing to avoid toxicity or irritation to the skin.

Korean Patent Registration No. 10-2036530 Korean Patent Publication No. 10-2021-0103951

An object of the present invention is to provide a cosmetic composition containing a probiotics culture and a plant extract as an active ingredient, which has a skin whitening or wrinkle improvement effect.

According to one aspect of the invention,

A medium after culturing lactic acid bacteria and a culture containing lactic acid bacteria cells; and

A cosmetic composition containing as an active ingredient a plant extract containing at least one extract selected from new green leaf, red mustard leaf, broccoli leaf, green mustard leaf, and broccoli peduncle/bud is provided.

The lactic acid bacteria may be strains of the genus Lactobacillus .

The strains of the genus Lactobacillus include Lactobacillus plantarum , Lactobacillus reuteri , Lactobacillus harbinensis , Lactobacillus casei , Lactobacillus sake ( Among Lactobacillus sakei ), Lactobacillus brevis , Lactobacillus pentosus , Lactobacillus fermentum , Lactobacillus acidophilus and Lactobacillus rhamnosus can be any one selected.

The plant extract may include extracts of new green leaves, red mustard leaves, and broccoli leaves.

The plant extract may be extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The medium may contain 1 to 10% (v/v) of deep sea water.

The cosmetic composition may include the culture and plant extract in a weight ratio of 10:1 to 10:5.

The cosmetic composition may be used for skin whitening or wrinkle improvement.

The cosmetic composition containing the probiotics culture and plant extract of the present invention inhibits the production of MMP-1 protein, promotes collagen production, and has an ability to inhibit tyrosinase and melanin production, so it has excellent skin whitening and wrinkle improvement effects.

Since the present invention can apply various transformations and have various embodiments, specific embodiments will be illustrated in the drawings and described in detail in the detailed description. However, it should be understood that this is not intended to limit the present invention to specific embodiments, and includes all transformations, equivalents, and substitutes included in the spirit and scope of the present invention. In describing the present invention, if it is determined that a detailed description of related known technologies may obscure the gist of the present invention, the detailed description will be omitted.

Hereinafter, the cosmetic composition of the present invention will be described.

The cosmetic composition of the present invention is a culture containing a culture medium and lactic acid bacteria cells after culturing lactic acid bacteria; and a plant extract including at least one extract selected from new green leaf, red mustard leaf, broccoli leaf, green mustard leaf, and broccoli peduncle/bud.

The culture is characterized in that the cultured lactic acid bacteria strain is used as an active ingredient of cosmetics without removing it.

The lactic acid bacteria are preferably strains of the genus Lactobacillus .

The strains of the genus Lactobacillus include Lactobacillus plantarum , Lactobacillus reuteri , Lactobacillus harbinensis, Lactobacillus casei , Lactobacillus sake ( Among Lactobacillus sakei ), Lactobacillus brevis , Lactobacillus pentosus , Lactobacillus fermentum , Lactobacillus acidophilus and Lactobacillus rhamnosus It is preferably any selected one, more preferably Lactobacillus plantarum ( Lactobacillus plantarum ).

The Lactobacillus plantarum is a bacterium that is safe enough to produce useful metabolites for the human body and to be used as a food raw material. The Lactobacillus plantarum culture of the present invention is obtained by culturing the Lactobacillus plantarum and disrupting the cells with a homogenizer, and contains metabolic components, cytoplasm, cell wall components, polysaccharides, etc. of Lactobacillus plantarum It is a fermentation lysate in

Newgreen (brassica oleracea var. italica) is one of the types of broccoli belonging to the mustard family and is also called Toscano. The difference is that broccoli eats the flower buds and peduncles, but Newgreen eats the leaves. The leaves are long oval, dark green, and are characterized by convex convex concavities on the front side.

Red mustard is a type of mustard greens and is characterized by its pungent spicy taste and aroma. In Korea, it is mainly eaten as a ssam vegetable or salad, and is also used to make kimchi. It is also called Gochae (高菜) or Jeokchae (赤高菜). The leaves are wide and round, and the leaf surface is crooked, and it looks very similar to the cap. The color of the leaves is magenta-tinged blue, blue-red, etc. Green mustard is called green mustard. It has strong cold tolerance and grows well in any soil. The phenomenon of promotion, in which the flower stalk rises and lowers the value of the product, also appears late. Harvesting can be done when the original height is about 40 cm and the main leaves grow about 8 to 10 pieces. It is known for its bright red color, vitality in veins, and freshness with luster, and rich in nutrients such as vitamins A and C, carotene, calcium, and iron.

Cheong mustard is also called Cheonggochae (靑高菜). The mustard leaf is a leaf that grows before the mustard fruit opens, and the edge of the leaf is crispy. It has strong cold tolerance and grows well in any soil. It is characterized by a pungent taste and fragrance. Its strong aroma removes the fishy taste and goes well with meat or fish dishes. The general characteristics are similar to red mustard.

Broccoli is a dark green vegetable belonging to the mustard family and is also called 'green cauliflower'. Broccoli is rich in antioxidants such as vitamin C and beta-carotene. Beta-carotene is a micronutrient that has antioxidant activity as a substance prior to the production of vitamin A. Antioxidants are known to prevent adult diseases such as aging, cancer, and heart disease by eliminating harmful oxygen accumulated in our body.

The plant extract preferably includes extracts of new green leaf, red mustard leaf and broccoli leaf. When these three types of extracts are combined, the wrinkle improvement or whitening effect is the best.

The plant extract may be extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, and preferably may be a hot water extract.

The medium for culturing the lactic acid bacteria preferably contains 1 to 10% (v/v) of the deep sea water, more preferably 2 to 8% (v/v), and more preferably 4 to 6% (v/v). v) may contain. If it is less than 1% (v / v), wrinkle improvement or skin whitening effect may be reduced, and if it exceeds 10% (v / v), it may be difficult to culture lactic acid bacteria as the salt concentration increases.

The cosmetic composition preferably includes the culture and plant extracts in a weight ratio of 10:2 to 10:8, and more preferably, a weight ratio of 10:4 to 10:6. Wrinkle improvement or skin whitening effect may be the highest in the above range.

The cosmetic composition of the present invention is characterized in that it is for skin whitening or wrinkle improvement.

The term "extract" used herein includes not only a crude extract obtained by treating an extraction solvent, but also a processed product of the extract. For example, the extract can be prepared in a powder state by additional processes such as vacuum distillation and freeze drying or spray drying.

In addition, the plant extract comprising at least one extract selected from new green leaf, red mustard leaf, broccoli leaf, green mustard leaf and broccoli peduncle/bud of the present invention includes a formulated processed product, such as plant powder, in a broad sense has the meaning of Although the experiment was conducted with plant extracts in the present invention, it will be expected by those skilled in the art that the desired effect can be achieved even in the form of processed plant extracts.

On the other hand, in the present specification, the term "comprising as an active ingredient" means including a sufficient amount to achieve the efficacy or activity of ingredients including probiotics culture and plant extracts. For example, it is used at a concentration of 10 to 1500 μg/ml, preferably 100 to 1000 μg/ml, including the probiotics culture and plant extract. Since the probiotics culture is a natural product and does not have side effects on the human body even when applied to the skin in excess, the quantitative upper limit of the probiotics culture and plant extract included in the composition of the present invention can be selected and implemented by those skilled in the art within an appropriate range.

The term "skin whitening" used in the present invention is to whiten the skin, and the skin color of a person is determined by the concentration and distribution of melanin inside the skin. In addition to genetic factors, solar ultraviolet radiation, fatigue, stress, etc. are also affected by environmental or physiological conditions. Melanin is synthesized in melanocytes in the epidermal layer of the skin. An enzyme called tyrosinase acts on tyrosine, a kind of amino acid, in melanosomes, organelles in melanocytes, to produce DOPA and dopaquinone. ) and then produced through a non-enzymatic oxidation reaction. When such melanin synthesis occurs excessively in the skin, the skin tone is darkened, and spots, freckles, and the like are generated. Therefore, if the synthesis of melanin pigment is inhibited by inhibiting the activity of tyrosinase in the skin, skin whitening can be realized by brightening the skin tone, as well as skin hyperpigmentation such as spots and freckles caused by ultraviolet rays, hormones, and genetic causes. It can improve depression.

In addition, the term "wrinkle" in the present invention refers to fine lines caused by skin deterioration, which may be caused by genes, reduction of collagen present in the skin dermis, external environment, and the like. In the present invention, "wrinkle improvement" refers to inhibiting or inhibiting the formation of wrinkles on the skin, or alleviating wrinkles already formed.

The main function of skin fibroblasts is to regenerate damaged skin tissue through extracellular matrix synthesis and proliferation. Extrinsic aging factors such as photoaging or endogenous aging factors such as accumulation of free oxygen damage and cell aging due to telomere disruption They lower the physiological activity of dermal fibroblasts in the dermal layer. Type I collagen, which accounts for more than 95% of skin extracellular matrix and plays a very important part in the physiological activity of fibroblasts through interaction and signal exchange with adhesion molecules and cytoskeleton of cells When the synthesis of collagen is reduced, the amount of collagen in the skin tissue is reduced, and as a result, the surface tension is reduced, resulting in a decrease in skin elasticity and formation of skin wrinkles, as well as a decrease in physiologically active functions including cell proliferation and regeneration. cause a vicious cycle of

Therefore, collagen of dermal fibroblasts can be directly related to skin regeneration, skin elasticity, skin wrinkle formation, and tissue repair or regeneration upon skin damage. That is, when the synthesis of collagen or procollagen in skin fibroblasts is promoted or the synthesis of matrix metallo-proteinase (hereinafter referred to as 'MMP') that degrades collagen is inhibited, skin elasticity is improved, skin regeneration, skin wrinkle improvement, wound healing, repair and regeneration of damaged skin tissue; And effects such as skin aging prevention can be obtained.

The cosmetic composition of the present invention is characterized by increasing the expression of type I procollagen protein and suppressing the expression of MMP-1 protein. Specifically, the cosmetic composition of the present invention suppresses the expression of matrix metallo-proteinase, which promotes collagen degradation, while increasing the production of type I procollagen, thereby increasing skin elasticity and regenerating skin. , skin wrinkle improvement, wound healing, repair and regeneration of damaged skin tissue; And it may have effects such as skin aging prevention. That is, it may have an effect of preventing or improving skin wrinkles.

In addition, the cosmetic composition of the present invention may further include water-soluble vitamins, fat-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extracts.

Any water-soluble vitamin may be used as long as it can be formulated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H and the like, and their salts (thiamin hydrochloride, ascorbic acid sodium salt, etc.) or derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) can also be used in the present invention. Included in water-soluble vitamins. The water-soluble vitamin can be obtained by conventional methods such as microbial transformation, microbial culture purification, enzymatic or chemical synthesis.

The fat-soluble vitamin may be any substance as long as it can be formulated into cosmetics, but preferably includes vitamin A, carotene, vitamin D2, vitamin D3, vitamin E, etc., and their derivatives (ascorbic acid palmitate, ascorbic acid stearate). Bean, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinate, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) are also included in the fat-soluble vitamins used in the present invention. do.

The polymer peptide may be anything as long as it can be incorporated into cosmetics, but preferably includes collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like. The polymeric peptide can be purified and obtained by a conventional method such as a microbial culture solution purification method, an enzyme method, or a chemical synthesis method, or can be used after being purified from a natural product such as pig or cow dermis or silkworm silk fiber.

The polymeric polysaccharide may be anything as long as it can be formulated into cosmetics, but preferably includes hydroxyethyl cellulose, xanthan gum, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be used after being purified from mammals or fish.

The sphingolipid may be any material as long as it can be formulated into cosmetics, but preferably includes ceramide, phytosphingosine, sphingosaccharide lipid, and the like. The sphingolipids can be purified by conventional methods or obtained by chemical synthesis from mammals, fish, shellfish, yeast or plants.

The seaweed extract may be anything as long as it can be formulated into cosmetics, but preferably brown algae extract, red algae extract, green algae extract, etc., and also calrageenan, arginic acid, and arginic acid purified from these seaweed extracts Sodium, potassium alginate, etc. are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purifying from seaweed by a conventional method.

In the cosmetic composition of the present invention, in addition to the above essential components, other ingredients commonly used in cosmetics may be mixed as necessary.

Other ingredients that may be added include fats and oils, humectants, emollients, ultraviolet absorbers, pH adjusters, fragrances, blood circulation promoters, cooling agents, antiperspirants, and purified water.

Examples of the oil and fat component include ester oil, hydrocarbon oil, silicone oil, fluorine oil, animal fat and vegetable oil.

Examples of the ester-based oils include tri-2-ethylhexanoate glyceryl, 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearate isocetyl, Butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, Diisopropyl adipate, isoalkyl neopentanoate, tri(capryl, capric acid) glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, tetraethyl 2-ethylhexanoate pentaelli Slitol, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, lauric acid sostearyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, 2-ethylhexanoate Tostearyl, 2-ethylstearyl hexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl dioctanoate Glycol, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyl neodecanoate Decyl, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleate, polyglycerin isostearate, triisocetyl citrate, triisoalkyl citrate, Triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, hydroxystearate 2- Ethylhexyl, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholenate oleate Steryl, Dihydrocholesteryl oleate, Physteryl isostearate, Physteryl oleate, Isocetyl 12-stealoylhydroxystearate, Stearyl 12-stealoylhydroxystearate, 12-stealo Ester systems, such as isostearyl monohydroxystearate, etc. are mentioned.

Examples of the hydrocarbon-based fats and oils include hydrocarbon-based fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybuden, microcrystalline wax, and vaseline.

Examples of the silicone-based oil include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane methylcetyloxysiloxane copolymer, dimethylsiloxane methylstealoxysiloxane copolymer, alkyl Modified silicone oil, amino modified silicone oil, etc. are mentioned.

Examples of the fluorine-based fats and oils include perfluoropolyether and the like.

The animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, cottonseed oil, palm oil, Kukui nut oil, wheat germ oil, rice germ oil, shea butter, moonshine colostrum, marker daemia nut oil, egg yolk oil, beef tallow, horse oil, mink oil, orange raffia oil, jojoba oil, candelilla wax, carnaba wax, liquid lanol , animal or vegetable fats and oils such as hydrogenated castor oil.

Examples of the moisturizer include water-soluble low-molecular moisturizers, fat-soluble molecular moisturizers, water-soluble polymers, and oil-soluble polymers.

The water-soluble low-molecular moisturizers include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), Polypropylene glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactate, etc. are mentioned.

Examples of the fat-soluble low-molecular moisturizers include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water-soluble chitin, chitosan, dextrin, etc. can be heard

Examples of the fat-soluble polymer include polyvinylpyrrolidone eicosene copolymer, polyvinylpyrrolidone hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, polymeric silicone, and the like.

Examples of the emollient agent include long-chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

The ultraviolet absorber includes para-aminobenzoic acid, para-aminobenzoic acid ethyl, para-aminobenzoic acid amyl, para-aminobenzoic acid octyl, salicylic ethylene glycol, salicylic acid phenyl, salicylic acid octyl, salicylic acid benzyl, salicylic acid butylphenyl, salicylic acid homomentyl, Benzyl cinnamate, paramethoxycinnamic acid-2-ethoxyethyl, paramethoxycinnamic acid octyl, diparamethoxycinnamic acid mono-2-ethylhexane glyceryl, paramethoxycinnamic acid isopropyl, diisopropyl-diisopropylcinnamic acid ester mixture , urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxy Benzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy) -1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl) benzotriazole, etc. are mentioned.

Examples of the pH adjusting agent include citric acid, sodium citrate, malic acid, sodium malate, fumalic acid, sodium fumalate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate, and the like.

Examples of the pH adjusting agent include citric acid, sodium citrate, malic acid, sodium malate, fumalic acid, sodium fumalate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate, and the like.

Cosmetics prepared by containing the cosmetic composition of the present invention may take the form of solutions, emulsions, viscous mixtures, and the like.

In addition, the ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the above ingredients as active ingredients, for example, conventional adjuvants such as stabilizers, pigments and natural flavors, and A carrier may be further included.

The cosmetic composition of the present invention can be used in various ways such as cosmetics or facial cleansers having skin whitening or wrinkle improvement effects.

Products to which the cosmetic composition of the present invention can be added include, for example, BB cream, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, and ultraviolet rays. There are cosmetics such as blocking cream, moisture cream, hand cream, foundation, essence, nutrient essence, and mask pack, as well as soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane , butane or a propellant such as dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

Hereinafter, preferred embodiments are presented to aid understanding of the present invention, but the following examples are merely illustrative of the present invention, and various changes and modifications are possible within the scope and spirit of the present invention. It is obvious to those skilled in the art, It goes without saying that these variations and modifications fall within the scope of the appended claims.

[Example]

Preparation Example 1: Preparation of New Green Leaf Extract

After cutting the dried New Green leaves finely and extracting with water at a ratio of 1:10 (w/v) at 95℃ for 3 hours, impurities were removed by filtering with a non-woven fabric, and concentrated under reduced pressure so that the solid content was 15% or more. A leaf extract was prepared.

Preparation Example 2: Preparation of red mustard leaf extract

A red mustard leaf extract was prepared in the same conditions as in Preparation Example 1, except that dried red mustard leaves were used instead of the dried new green leaves.

Preparation Example 3: Preparation of broccoli leaf extract

A broccoli leaf extract was prepared under the same conditions as in Preparation Example 1, except that dried broccoli leaves were used instead of dried new green leaves.

Preparation Example 4: Preparation of green mustard leaf extract

A green mustard leaf extract was prepared under the same conditions as in Preparation Example 1, except that dried green mustard leaves were used instead of the dried new green leaves.

Preparation Example 5: Preparation of broccoli peduncle/bud extract

Broccoli peduncle/bud extract was prepared under the same conditions as in Preparation Example 1, except that dried broccoli peduncle/bud was used instead of dried new green leaves.

Preparation Example A: Preparation of Lactobacillus culture using deep sea water

Lactobacillus plantarum strain was inoculated with 1 ml of Lactobacillus plantarum 10 ⁸ CFU/ml) in a mixed medium mixed with 95 ml of MRS Broth liquid medium and 5 ml of deep sea water, and incubated at 37 ° C for 30 hours. After culturing, the culture was disrupted with a homogenizer to obtain a Lactobacillus plantarum culture.

Preparation Example B: Preparation of Lactobacillus culture filtrate using deep sea water

After culturing, instead of disrupting the cells with a homogenizer, the culture was cultured under the same conditions as in Preparation Example A except that the filtrate obtained by removing the cells was obtained by centrifuging the culture.

Preparation Example C: Preparation of Lactobacillus Culture

A Lactobacillus plantarum culture was obtained under the same conditions as in Preparation Example A, except that 100 ml of MRS Broth liquid medium was used instead of the mixed medium mixed with 95 ml of MRS Broth liquid medium and 5 ml of deep sea water.

Example 1: Lactobacillus plantarum culture + plant extract complex

A probiotics + plant complex was prepared by mixing the new green leaf extract prepared according to Preparation Example 1 and the Lactobacillus culture using deep sea water prepared according to Preparation Example A at a weight ratio of 3:7.

Example 2: Lactobacillus plantarum culture + plant extract complex

A probiotics + plant complex was prepared by mixing the red mustard leaf extract prepared in Preparation Example 2 and the Lactobacillus culture using deep sea water prepared in Preparation Example A at a weight ratio of 3:7.

Example 3: Lactobacillus plantarum culture + plant extract complex

A probiotics + plant complex was prepared by mixing the broccoli leaf extract prepared in Preparation Example 3 and the Lactobacillus culture using deep sea water prepared in Preparation Example A at a weight ratio of 3:7.

Example 4: Lactobacillus plantarum culture + plant extract complex

A probiotics + plant complex was prepared by mixing the mustard leaf extract prepared in Preparation Example 4 and the Lactobacillus culture using deep sea water prepared in Preparation Example A at a weight ratio of 3:7.

Example 5: Lactobacillus plantarum culture + plant extract complex

A probiotics + plant complex was prepared by mixing the broccoli flower stalk/bud extract prepared in Preparation Example 5 and the Lactobacillus culture using deep sea water prepared in Preparation Example A at a weight ratio of 3:7.

Example 6: Lactobacillus plantarum culture + plant extract complex

The new green leaf extract of Preparation Example 1, the red mustard leaf extract of Preparation Example 2, and the Lactobacillus culture using the deep sea water prepared according to Preparation Example A were mixed at a weight ratio of 1.5: 1.5: 7 to prepare a probiotics + plant complex. .

Example 7: Lactobacillus plantarum culture + plant extract complex

The new green leaf extract of Preparation Example 1, the broccoli leaf extract of Preparation Example 3, and the Lactobacillus culture using the deep sea water of Preparation Example A were mixed at a weight ratio of 1.5: 1.5: 7 to prepare a probiotics + plant complex.

Example 8: Lactobacillus plantarum culture + plant extract complex

The new green leaf extract of Preparation Example 1, the red mustard extract of Preparation Example 2, the broccoli leaf extract of Preparation Example 3, and the Lactobacillus culture using the deep sea water of Preparation Example A were mixed at a weight ratio of 1: 1: 1: 7 to obtain probiotics. +Plant complexes were prepared.

Example 9: Lactobacillus plantarum culture + plant extract complex

The new green leaf extract of Preparation Example 1, the green mustard extract of Preparation Example 4, the broccoli leaf extract of Preparation Example 3, and the Lactobacillus culture using the deep sea water of Preparation Example A were mixed at a weight ratio of 1:1:1:7 to obtain probiotics. +Plant complexes were prepared.

Example 10: Lactobacillus plantarum culture + plant extract complex

New green leaf extract of Preparation Example 1, red mustard extract of Preparation Example 2, broccoli peduncle/bud extract of Preparation Example 5, Lactobacillus culture using deep sea water of Preparation Example A at a weight ratio of 1: 1: 1: 7 By mixing, a probiotics+plant complex was prepared.

Example 11: Lactobacillus plantarum culture + plant extract complex

Lactobacillus culture using the new green leaf extract of Preparation Example 1, the mustard extract of Preparation Example 4, the broccoli peduncle/bud extract of Preparation Example 5, and the deep sea water of Preparation Example A at a weight ratio of 1: 1: 1: 7 By mixing, a probiotics+plant complex was prepared.

Example 12: Lactobacillus plantarum culture + plant extract complex

The new green leaf extract of Preparation Example 1, the red mustard extract of Preparation Example 2, the broccoli leaf extract of Preparation Example 3, and the Lactobacillus culture of Preparation Example C without deep sea water were mixed at a weight ratio of 1: 1: 1: 7 Thus, a probiotics + plant complex was prepared.

Comparative Example 1: Lactobacillus plantarum culture using deep sea water

Except for the plant extract, the Lactobacillus culture using the deep sea water of Preparation Example A was used alone.

Comparative Example 2: Plant Extract

Except for the Lactobacillus culture, a plant extract obtained by mixing the new green leaf extract of Preparation Example 1, the red mustard extract of Preparation Example 2, and the broccoli leaf extract of Preparation Example 3 at a weight ratio of 1:1:1 was used alone.

Comparative Example 3: Lactobacillus plantarum culture using deep sea water + plant extract medium

Instead of a mixed medium in which 95 ml of MRS Broth liquid medium and 5 ml of deep sea water were mixed, 90 ml of MRS Broth liquid medium, 5 ml of deep sea water, and mixed plant extracts (new green leaf of Preparation Example 1, red mustard leaf of Preparation Example 2, preparation Broccoli leaves of Example 3) A culture medium of Lactobacillus plantachum cultured under the same conditions as in Preparation Example A was prepared, except for using a mixed medium in which 5 ml was mixed. That is, the culture was prepared by including the plant extract in the Lactobacillus culture medium.

Comparative Example 4: Lactobacillus plantarum culture filtrate + plant extract complex

A probiotics + plant composite was prepared under the same conditions as in Example 8, except that the Lactobacillus culture filtrate prepared according to Preparation Example B was used instead of the Lactobacillus culture of Preparation Example A.

The manufacturing conditions of Examples 1 to 12 and Comparative Examples 1 to 4 are summarized in Table 1 below.

division Lactobacillus culture medium conditions Inclusion of culture cells plant extract deep sea water mixed plant extract Example 1 include not included include new green leaves Example 2 include not included include red mustard leaves Example 3 include not included include broccoli leaves Example 4 include not included include green mustard leaves Example 5 include not included include Broccoli peduncle/bud Example 6 include not included include New green leaf + red mustard leaf Example 7 include not included include New green leaves + broccoli leaves Example 8 include not included include New green leaf + red mustard leaf + broccoli leaf Example 9 include not included include New green leaf + green mustard leaf + broccoli leaf Example 10 include not included include New green leaf + red mustard leaf + broccoli peduncle/bud Example 11 include not included include New green leaf + green mustard leaf + broccoli peduncle/bud Example 12 not included not included include New green leaf + red mustard leaf + broccoli leaf Comparative Example 1 include not included include not included Comparative Example 2 - - - New green leaf + red mustard leaf + broccoli leaf Comparative Example 3 include include include not included Comparative Example 4 include not included not included New green leaf + red mustard leaf + broccoli leaf

[Experimental example]

All statistical analyzes were performed using SPSS version 18.0 (IBM, Chicago, IL, USA). A one-way ANOVA with Duncan's post-hoc test was used to determine the difference (p <0.05) in mean values between the experimental groups. Data from each experiment were expressed as mean ± standard deviation (SD).

Experimental Example 1: Cytotoxicity assay

Cytotoxicity was measured using the MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction method. The cultured HaCaT cells were mixed well in DMEM medium, adjusted to 1x10 ⁵ cells/mL, and then 1 mL of each prepared cell was added to a 24-well culture plate and cultured for 3 days. When the cells grew to about 80%, the samples (0, 25, 50, 100, 300, 400, 500, 1000 μg/ml) of Examples 1 to 4 were treated in a medium without the addition of fetal bovine serum and antibiotics by concentration After that, the culture was further cultured for 24 hours, and the viability of the cultured cells was measured using the MTT reduction method.

After adding about 1/10 of the volume of the growth medium to each well, the MTT solution (5 mg/ml) was further incubated at 37 °C for 4 hours. Thereafter, the medium was removed while being careful not to lose the formazan produced by reducing MTT, and after leaving the medium at room temperature for 30 minutes to completely remove the remaining medium, the sample was dissolved using DMSO and then 570 nm The absorbance was measured at. Cell viability was calculated according to Equation 1 below, and the results are shown in Table 4.

[Equation 1]

Cell viability (%) = [absorbance of sample treatment group/absorbance of control group] x 100

division Concentration (μg/ml) 10 100 untreated 100±0.8 Example 1 98.2±1.3 94.8±1.3 Example 2 97.7±1.2 91.5±0.9 Example 3 96.2±1.0 93.4±0.8 Example 4 97.0±1.4 92.3±1.1

According to this, it was confirmed that the probiotics + plant extract complex prepared according to Examples 1 to 4 of the present invention can be safely used on human skin. Since the components of Examples 5 to 12 and Comparative Examples 1 to 4 were included in any one of the components of Examples 1 to 4, a separate cytotoxicity test was not conducted.

Experimental Example 2: MMP-1 synthesis inhibitory effect analysis

CCD-986sk was cultured in a 35 mm dish at a concentration of 1.5X10 ⁵ cells/ml until reaching about 80% confluency. After removing the original medium, washing with PBS to remove the serum component in the medium, UVA (6.3J/cm2) was irradiated for 24 hours using a UV light source (Coralife, 35W, UV) equipped with a filter. . After UVA irradiation, the samples of Examples or Comparative Examples of the present invention were administered at concentrations of 10 and 100 μg/ml in DMEM medium without FBS, respectively, and cultured for 24 hours. The cultured medium was dispensed into a 96 well plate and reacted overnight at 4 °C. MMP-1 expression level induced by UVA irradiation was measured using the method used by Dunsmore et al.

First, the medium was washed three times with TBS (phosphate bufferedsaline + 0.05% Tween20), and then blocked with 3% PBS at 37 °C for 1 hour. Thereafter, a solution diluted with the primary antibody (monoclonal anti-MMP-1 antibody) in a blocking buffer at a volume ratio of 1:3000 was added and reacted at 37° C. for 90 minutes. Thereafter, after washing with PBS, a solution in which a secondary antibody (alkaline phosphatase conjugated Goat anti-mouse IgG) was diluted with a blocking buffer at a volume ratio of 1:3,000 was added and reacted at 37° C. for 90 minutes. Thereafter, after washing with PBS, alkaline phosphatase substrate solution (1 mg/ml, p-mitrophenylphosphate in diethanolamine buffer) was added, followed by reaction at room temperature for 30 minutes, and then the reaction was stopped with 3N NaOH. Next, absorbance was measured at 405 nm using a microplate reader, and the results are shown in Table 5 below.

division Concentration (μg/ml) 10 100 untreated 8812±12 Example 1 5966±82 4830±92 Example 2 6502±102 5331±46 Example 3 6430±47 5520±72 Example 4 6493±57 5299±89 Example 5 5864±88 4931±80 Example 6 5090±101 3828±95 Example 7 5989±46 4430±122 Example 8 4730±42 3202±34 Example 9 5600±48 4791±94 Example 10 5384±102 4028±94 Example 11 5764±74 5003±79 Example 12 5866±87 4831±90 Comparative Example 1 7862±53 7202±48 Comparative Example 2 7963±55 7421±39 Comparative Example 3 7063±98 6701±76 Comparative Example 4 7263±112 6223±106

According to this, the sample containing the probiotics culture using the three kinds of plant extracts of new green leaf + red mustard leaf + broccoli leaf and deep sea water of Example 8 showed the highest synthesis inhibitory effect of MMP-1 protein. The sample of Example 12 containing the same composition as but the probiotics culture cultured without using deep sea water was measured to have a relatively low inhibitory effect on the synthesis of MMP-1 protein compared to Example 8. In addition, Comparative Example 1 including a probiotics culture cultured using deep sea water but excluding plant extracts and Comparative Example 2 having three plant extracts including new green leaf + red mustard leaf + broccoli leaf but excluding the probiotics culture The synthesis inhibitory effect of MMP-1 protein was relatively the lowest. In addition, a high level of MMP-1 protein synthesis inhibitory effect was shown in samples containing probiotics cultures and plant extracts together.

Experimental Example 3: Collagen synthesis promotion effect analysis

In order to confirm the skin wrinkle improvement effect of the cosmetic compositions of Examples and Comparative Examples, the amount of protein collagen produced to maintain skin elasticity was measured. Fibroblasts were dispensed at a density of 1X10 ⁴ cells/well in a 48 well plate, cultured for 24 hours in DMEM medium containing 10% FBS, and samples of Examples or Comparative Examples were added at 50 and 100 μg/ml, and no Incubated for an additional 24 hours in serum medium. After incubation, the supernatant of each well was collected and the amount of procollagen type I C-peptide (PICP) was measured using the procollagen type I C-peptide EIA kit (MK101, Takara, Japan) and converted into ng/ml. , The amount of collagen synthesized thereby was measured, and the results are shown in Table 4.

division Concentration (μg/ml) 50 100 untreated 39.2±0.3 Example 1 70.4±0.5 79.3±0.5 Example 2 64.1±0.3 74.1±0.9 Example 3 63.4±0.7 73.2±0.3 Example 4 62.3±0.4 71.7±0.8 Example 5 60.1±0.3 73.1±0.9 Example 6 77.2±0.4 86.2±0.5 Example 7 68.1±0.9 78.2±1.2 Example 8 80.2±1.3 91.2±0.6 Example 9 67.2±0.4 76.2±0.2 Example 10 75.8±0.8 85.0±0.8 Example 11 65.6±0.6 77.2±1.1 Example 12 68.5±0.4 74.9±0.2 Comparative Example 1 49.2±0.6 54.2±0.5 Comparative Example 2 48.9±1.8 50.5±0.9 Comparative Example 3 51.4±0.4 56.2±0.7 Comparative Example 4 53.0±1.2 58.5±0.2

According to this, the sample containing the probiotics culture using the three kinds of plant extracts of new green leaf + red mustard leaf + broccoli leaf and deep sea water of Example 8 of the present invention was found to have the best collagen production promoting effect. The sample of Example 12, which had the same composition as Example 8 but contained a probiotics culture cultured without using deep sea water, showed a relatively low collagen production promoting effect compared to Example 8. On the other hand, in the samples of the examples containing the probiotics culture and plant extracts, the collagen production promoting effect was generally higher than that of the comparative examples.

Experimental Example 4: Analysis of tyrosinase inhibitory activity

The tyrosinase inhibitory ability of the cultures of Examples and Comparative Examples was measured by the method of Yagi et al. (1986). Melanin exists in the basal layer of the epidermis, and melanin synthesis is regulated by tyrosinase in melanocyte intracellular melanocytes. Therefore, inhibiting tyrosinase activity can reduce melanin synthesis and pigmentation. 0.2 ml of 110 Unit/ml mushroom tyrosinase was added to a mixture containing 0.2 ml of a substrate solution in which 10 mM L-DOPA was dissolved in 0.5 ml of 1.15M sodium phosphate buffer at pH 6.8 and 0.1 ml of a sample solution in a test tube. After reacting at 25 ° C. for 2 minutes, the absorbance of DOPA chrome generated in the reaction solution was measured at 490 nm using a microplate reader. The tyrosinase inhibitory activity was expressed as the rate of decrease in absorbance of the sample solution added group and non-added group, and the results are shown in Table 5 below.

division Treatment concentration (% (w/v)) 50 100 Example 1 5.8±0.3 9.2±0.6 Example 2 5.1±0.6 8.8±0.6 Example 3 4.8±0.5 8.1±0.9 Example 4 5.2±0.5 8.4±0.4 Example 5 5.4±0.7 9.0±0.6 Example 6 6.9±0.3 10.2±0.8 Example 7 5.9±0.4 9.2±0.4 Example 8 7.2±0.5 11.6±1.1 Example 9 6.2±0.4 9.8±0.3 Example 10 7.0±0.9 10.0±0.4 Example 11 6.3±0.5 9.3±0.4 Example 12 5.4±0.8 8.8±0.4 Comparative Example 1 3.2±0.5 6.3±0.8 Comparative Example 2 2.8±0.9 6.0±0.4 Comparative Example 3 3.5±0.2 6.8±0.7 Comparative Example 4 3.8±1.2 7.3±0.4

According to this, the sample containing the probiotics culture using the three kinds of plant extracts of new green leaf + red mustard leaf + broccoli leaf of Example 8 of the present invention and deep sea water was found to have the best tyrosinase inhibitory effect, The sample of Example 12 containing the same composition as Example 8 but the probiotics culture cultured without using deep sea water showed a relatively low tyrosinase inhibitory effect compared to Example 8. On the other hand, in the samples of Examples containing both the probiotics culture and the plant extract, the tyrosinase inhibitory activity was generally higher than that of Comparative Examples.

Experimental Example 5: Analysis of melanin production inhibitory ability

Mouse-derived melanocyte B16F1 cells produce melanin, and melanin is biosynthesized in the melanosome, an organelle within the melanocyte present in the basal layer of the epidermis, and the amount of melanin produced can be compared by measuring the absorbance in the visible light range. After inoculation of B16F1 cells, the sample was treated for 3 days, the medium was removed, the cells were washed with PBS, 1 ml of 1 N NaOH was added to each well, and the melanin component was dissolved by stirring to melt the cell membrane, and the visible light range The amount of melanin produced was measured by measuring the absorbance at 400 nm, which is the shortest wavelength in the blue visible ray region.

The amount of melanin was measured by the method of expressing the absorbance per unit cell number (10 ⁶ cell), and the relative amount of melanin production with respect to the control group (untreated group) was calculated as an inhibition rate (%), and the results are summarized in Table 6.

Classification (unit: %) Concentration (μg/ml) 50 100 untreated 41.2±0.5 Example 1 55.6±0.2 62.6±0.5 Example 2 50.1±0.5 58.6±0.9 Example 3 52.3±0.8 57.2±0.3 Example 4 50.1±0.8 60.8±0.9 Example 5 51.8±0.4 58.9±0.6 Example 6 59.4±0.3 69.0±0.8 Example 7 55.9±0.7 62.4±0.4 Example 8 63.2±0.8 72.5±0.5 Example 9 56.5±0.4 62.4±1.2 Example 10 57.8±0.9 67.5±0.7 Example 11 58.9±0.4 63.9±0.3 Example 12 55.9±0.7 61.9±0.4 Comparative Example 1 40.8±0.3 49.1±0.6 Comparative Example 2 38.8±0.4 46.2±0.5 Comparative Example 3 41.6±1.2 50.2±0.4 Comparative Example 4 39.8±0.4 48.2±0.5

According to this, the sample containing the probiotics culture using the three kinds of plant extracts of new green leaf + red mustard leaf + broccoli leaf and deep sea water of Example 8 of the present invention was found to have the best melanin production inhibitory ability, and Example 8 The sample of Example 12 containing the same configuration as but the probiotics culture cultured without using deep sea water showed a relatively low melanin production inhibitory ability compared to Example 8. On the other hand, in the samples of Examples containing both the probiotics culture and the plant extract, melanin production inhibitory activity was measured to be generally higher than those of the Comparative Examples.

Formulation examples of cosmetic compositions suitable for application of the present invention are presented.

Formulation Example 1: Lotion

Formulation examples of lotion using the cosmetic composition of Example 8 are shown in Table 7 below.

ingredient Content (% by weight) Composition of Example 8 5.0 glycerin 6.0 1,3-butylene glycol 3.0 PEG 1500 1.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 sodium hyaluronate 5.0 ethanol 10.0 Polysorbate 20 0.2 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 2: Lotion

Formulation examples of lotions using the cosmetic composition of Example 8 are shown in Table 8 below.

ingredient Content (% by weight) Composition of Example 8 3.0 propylene glycol 6.0 glycerin 4.0 triethanolamine 1.2 Tocopheryl Acetate 3.0 liquid paraffin 5.0 squalane 3.0 macadamia nut oil 2.0 polysorbate 60 1.5 Sorbitan sesquioleate 1.0 carboxyvinyl polymer 1.0 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 3: BB Cream

Formulation examples of BB cream using the cosmetic composition of Example 8 are shown in Table 9 below.

ingredient Content (% by weight) Composition of Example 8 1.0 Pro-type glycerin monostearate 1.5 cetearyl alcohol 1.5 stearic acid 1.0 polysorbate 60 1.5 Sorbitan Stearate 0.6 isostearyl isostearate 5.0 squalane 5.0 mineral oil 35.0 dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 triethanolamine 0.7 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 4: Essence

Formulation examples of essence using the cosmetic composition of Example 8 are shown in Table 10 below.

ingredient Content (% by weight) Composition of Example 8 1.5 glycerin 10.0 betaine 5.0 PEG 1500 2.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl Cellulose 0.1 sodium hyaluronate 8.0 carboxyvinyl polymer 0.2 triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeces-16 0.4 ethanol 6.0 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 5: Emulsion for mask pack

Formulation examples of emulsions for mask packs using the cosmetic composition of Example 8 are shown in Table 11 below.

ingredient Content (% by weight) Composition of Example 8 1.0 polyvinyl alcohol 15.0 cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 cyclodextrin 0.15 DL-panthenol 0.4 allantoin 0.1 Monoammonium glycyrrhizinate 0.3 nicotinamide 0.5 ethanol 6.0 PEG 40 hydrogenated castor oil 0.3 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Although the embodiments of the present invention have been described above, those skilled in the art can add, change, delete, or add components within the scope not departing from the spirit of the present invention described in the claims. The present invention can be variously modified and changed by the like, and this will also be said to be included within the scope of the present invention.

Claims (8)

A medium after culturing lactic acid bacteria and a culture containing lactic acid bacteria cells; and
A cosmetic composition comprising, as an active ingredient, a plant extract comprising at least one extract selected from new green leaf, red mustard leaf, broccoli leaf, green mustard leaf, and broccoli peduncle/bud. According to claim 1,
The lactic acid bacteria are Lactobacillus ( Lactobacillus ) Cosmetic composition, characterized in that the genus strain. According to claim 1,
The strains of the genus Lactobacillus include Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus harbinensis, Lactobacillus casei, Lactobacillus sake ( Among Lactobacillus sakei), Lactobacillus brevis, Lactobacillus pentosus, Lactobacillus fermentum, Lactobacillus acidophilus and Lactobacillus rhamnosus A cosmetic composition, characterized in that the selected one. According to claim 1,
The plant extract is a cosmetic composition, characterized in that it comprises extracts of new green leaves, red mustard leaves and broccoli leaves. According to claim 4,
The plant extract is a cosmetic composition, characterized in that extracted by water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. According to claim 1,
The medium is a cosmetic composition, characterized in that it contains 1 to 10% (v / v) of deep sea water. According to claim 1,
The cosmetic composition is a cosmetic composition, characterized in that the culture and plant extracts are included in a weight ratio of 10: 1 to 10: 5. According to claim 1,
The cosmetic composition is a cosmetic composition, characterized in that for skin whitening or wrinkle improvement.