KR20160045289A - Cosmetic Composition for Whitening of the Skin Comprising the Extract of Fermented Zea Mays Silk - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising fermented liquid of corn silk obtained by culturing corn silk with Saccharomyces cerevisiae or lactobacillus for skin whitening, and to a method of applying makeup. The fermented liquid of corn silk according to the present invention has an excellent melanogenesis controlling effect and a tyrosinase activity inhibiting effect, and the skin whitening cosmetic composition comprising the fermented liquid of corn silk of the present invention has an excellent skin whitening effect.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for skin whitening comprising a fermentation liquid of corn steep liquor (Cosmetic Composition for Whitening of the Skin Comprising the Extract of Fermented Zea Mays Silk)

The present invention relates to a cosmetic composition for skin whitening comprising a corn steep liquor. More particularly, the present invention relates to a cosmetic composition for skin whitening comprising a corn steep liquor obtained by culturing corn beard with yeast or lactic acid bacteria.

Factors that determine skin color are basically skin color, such as tingling caused by spots, freckles and ultraviolet rays, or overall pigmentation, as well as differences in the distribution of acne and scarring and keratin Blood circulation, stress, and health. Among the above factors, the most important factor is the pigmentation. Melanin, melasoids, carotene, hemoglobin, carotenoids and the like are the coloring matters that affect the color of the skin. Various colors of skin, hair and eyes are determined by these pigments. The most important factor determining the color of the skin is melanin. The most important factors affecting the biosynthesis of melanin are ultraviolet light and hormone secretion in the body. Melanin absorbs or scatters ultraviolet light and plays a major role in preventing skin damage from ultraviolet rays. It does not have a specific maximum absorption wavelength and absorbs light in all areas. In addition, it has excellent ability to remove reactive oxygen species, sometimes melanin itself generates active oxygen, and other substances are reduced or oxidized by catechol or quinone in the melanin structure, and melanin itself shows free radical properties Pray.

The biosynthesis of melanin begins with the oxidation of tyrosine, a type of amino acid, into melanosomes of melanocytes and is converted to dihydroxyphenylalanine by tyrosinase, followed by a series of oxidation steps, starting with pheoelanin, It is formed of a polymer of eumelanin. This biosynthesis process is carried out in a special form of melanocyte in the brown intracellular organelle. The melanomas, including melanin granules, migrate from the nucleus to the tip of the dendritic process, and migrate into the cytoplasm by phagocytosis of keratinocytes. It accumulates around the nucleus.

The synthesis of melanin, the number of melanosomes, and the migration to keratinocytes in the periphery are partially affected by hormones and ultraviolet rays, and are primarily affected by genetics. In addition, it is known that tyrosinase expression, melanin synthesis, and intracellular regulatory factors such as cytokine, metal ions such as copper, zinc and iron, and interferon, prostaglandin, and histamine are involved.

Meanwhile, in recent years, many cosmetics companies are making efforts to develop skin whitening ingredients, and the active ingredients obtained through fermentation are also applied to products.

For example, there is a case where a cosmetic composition is provided using a fermentation broth produced using a fermented product of Bacillus subtilis used in a typical fermented food such as Chungkookjang and natto. These fermented liquids have advantages of degradation of skin irritation due to decomposition of stimulating substances by microorganisms or conversion into other substances, and fermentation products by microorganisms are also widely used because they have a good effect on skin.

The present inventors have also developed a product using natural materials for this purpose and have studied the effect of corn bean extract, which is known to help skin whitening, to be more effective than conventional corn musth extract.

As a result of screening various skin physiological activities of natural plant extracts in order to improve skin pigmentation, the present inventors have found that a corn steep liquor obtained by culturing a corn bean in a natural plant extract with yeast or lactic acid bacteria has a tyrosinase inhibitory effect and melanin Production inhibitory effect, skin pigmentation, and the like, thereby effectively improving skin whitening, thereby completing the present invention.

It is an object of the present invention to provide a cosmetic composition for skin whitening comprising a corn steep liquor.

Another object of the present invention is to provide a makeup method characterized by coating a skin whitening cosmetic composition comprising a corn steep liquor fermentation broth to inhibit tyrosinase activity and exhibit melanin production inhibition and whitening effect .

The above object is achieved by a cosmetic composition for skin whitening comprising a corn steep liquor obtained by culturing corn beard with yeast or lactic acid bacteria.

Preferably, the corn steep liquor is a fermentation broth obtained by cultivating corn steat using Saccharomyces cerevisiae as a yeast or Bifidobacterium as a lactic acid bacterium.

Preferably, the corn steep liquor fermentation broth is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition.

Preferably, the corn steep liquor fermentation liquid is characterized by inhibiting melanin production and inhibiting tyrosinase activity.

Preferably, the cosmetic composition is in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray ≪ / RTI >

The cosmetic composition of the present invention contains a fermentation broth obtained by culturing corn steat with yeast or lactic acid bacterium as an effective ingredient, thereby exhibiting an excellent whitening effect because it has excellent melanin production inhibitory effect and tyrosinase activity inhibiting effect. In addition, the cosmetic composition containing the fermented corn steep liquor of the present invention is superior in whitening effect to the conventional cosmetic composition containing the corn musth extract.

The present invention provides a cosmetic composition for skin whitening comprising a fermented corn steep liquor.

The corn beard used as an active ingredient in the composition of the present invention is corn ( Zea mays Linn ) and the pistil head (head: stigma).

It is a collection of beards of corn which is native to tropical America. This medicine has a peculiar odor, taste is sweet and cool, and the quality is flat without any bias. It is used for urination, edema caused by pyelonephritis, urinary incontinence, jaundiced hepatitis, cholecystitis, gallstones, and hypertension. The pharmacological effects of diuretic, bile secretion promotion, blood pressure and blood glucose lowering, has been reported.

The shape of the corn beard is a thin thread or a hair-like tangled mass, which is light green to yellowish green or yellowish brown. The vagina is soft, very light and glossy. Tinnitus, jade beans, jade beans, burdock beans, oak bean jam, 玉米 등, and 玉 头 are some of the tinnitus. . Compound leaves come out.

Until recently, corn beard has been used in the form of dry powder or in water or honey to be used for facial massage, or as a corn barrel that has been boiled with a mixture of corn beard and lemon juice.

As used herein, the term " corn beard " preferably refers to the corolla and stigma.

The corn steep liquor used in the present invention is characterized by being a corn steep liquor obtained by culturing corn steat using yeast or lactic acid bacteria.

The yeast cells used for fermenting corn steep livers of the present invention belong to eukaryotic microorganisms (organisms divided into nuclear and cytoplasmic nuclear membranes) and have cell organelles such as cell walls, cell membranes, nuclei, mitochondria, and granules. Those who have no distinction of sex mainly reproduce by budding. Among many separated yeasts, there is no effective ineffect, proliferation by fission or bud-fission method instead of the method of propagation, injection which is not ascospore There are also diverse groups of yeasts, including those that form spores (ballistospores) or that do not exhibit spore formation. The size is usually 5-10 × 5-12 μm, which is 5 to 10 times larger than the bacterium, and the optimum pH is 4.5 to 5.5, but it survives even at the pH 1 to pH 10. Yeast surviving at pH 1 for 12 hours is stronger on acid than bacteria, so adjusting the pH of the medium to about 3.5 ~ 3.8 can inhibit bacterial contamination to some extent.

Yeast is slightly different depending on the species, but most of them grow well at 20 ~ 25 ℃. Normally, it is said that it will die at above 50 ℃, but it can be overcome to some extent by adding directly the growth factor which can not be synthesized above the temperature. In the case of Saccharomyces cerevisiae, it can be prevented by adding oleic acid and ergosterol to the medium at 40 ° C or higher. Yeast cells themselves are unicellular plants and contain large amounts of proteins and trace minerals. A preferred yeast for use in fermenting corn beans of the invention is Saccharomyces cerevisiae.

The lactic acid bacterium used for fermenting the corn salad of the present invention is a kind of microorganism useful for the human body and is a bacterium that decomposes carbohydrates such as glucose or lactose to produce an organic acid such as lactic acid or acetic acid. Lactic acid bacteria make lactic acid from sugar is called fermentation, and through this fermentation process, fermented food is produced. In the present invention, Bifidobacterium was used in representative lactic acid bacteria (Lactobacillus, Streptococcus, Bifidobacterium, Leuconostock, Pediococcus, Lactococcus).

In the present invention, a fermented corn steep liquor is prepared as follows:

a) making the corn barb powder fine using 100-500 mesh;

b) adding 1 to 50 g / L of the corn steep liquor prepared in step a) to a conventional microorganism culture solution, adding yeast strain or lactic acid bacteria in an amount of 10,000 to 100,000 cfu / L;

c) culturing the culture of step b) at 30-37 ° C in aerobic or anaerobic conditions at pH 5-7 for about 5-10 days; And

d) filtering the culture of step c) and aging at low temperature for 5 to 10 days.

According to a preferred embodiment of the present invention, the corn steep liquor fermentation broth used in the present invention was prepared as follows:

Corn starch powder was pulverized to 300 mesh and added to the culture solution at 10 g / L. Yeast strain or lactic acid bacteria were added at 50,000 cfu / L, and the mixture was aerobically or tuber- And cultured in a fermenter for about 7 days under the condition. After culturing, a culture solution is obtained, and then the culture solution is filtered using 0.25 μM filter paper. The filtrate is further aged at low temperature for 10 days, filtered and concentrated to prepare the corn steep liquor of the present invention.

In the present invention, the fermented corn steep liquor is characterized by inhibiting tyrosinase activity and improving skin whitening inhibiting melanin production.

In the present invention, the content of the corn steep liquor fermentation broth is 0.0001 to 30.0% by weight, more preferably 0.001 to 20% by weight, based on the whole cosmetic composition. When the content of the fermentation broth of corn steep liquor is less than 0.0001% by weight, it can not be expected to improve the skin whitening effect. When the content of corn steep liquor fermentation broth exceeds 30.0% by weight, And there is a problem in the stability of the formulation.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to the corn steep liquor as an active ingredient and include conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, And a carrier.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be manufactured in the form of a flexible lotion (skin), a nutritional lotion (milk lotion), a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder .

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters.

The present invention also provides a makeup method characterized in that a skin whitening cosmetic composition comprising a corn steep liquor fermentation liquid is applied to human skin to inhibit tyrosinase activity and inhibit melanin production.

The cosmetic process of the present invention refers to all the cosmetic processes for applying the cosmetic composition of the present invention to human skin. That is, all methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. The cosmetic composition having an excellent skin whitening effect according to the present invention can be used according to a conventional method of use, and may be used in a number of times depending on the skin condition or taste of the user.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

When the cosmetic composition comprising the corn steep liquor fermentation broth of the present invention is applied to human skin, tyrosinase activity and melanin production can be suppressed, skin pigmentation can be improved, and skin whitening effect can be obtained.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Manufacturing example  1. Preparation of yeast fermented maize hull using yeast

After washing with purified water and drying, the corn hairs were pulverized and finely ground using 300 mesh. This was added to a yeast culture solution (Potato Dextrose Broth, Akyumedia, USA) at 10 g / L. The yeast strain used for fermentation was Saccharomyces cerevisiae , which was added at 50,000 cfu / L per culture. The cultures were maintained at 30 ° C and pH 5-7 for 7 days using a 5L fermenter. After culturing, the culture was centrifuged, and the culture was firstly removed and finally filtered using 0.25 μM filter paper. The filtrate thus obtained was aged for about 10 days at a low temperature warehouse at 4 DEG C, and the filtrate was filtered again using a 0.25 mu M filter and used in the present invention. The thus prepared fermentation broth is hereinafter referred to as " corn bean yeast fermentation broth. &Quot; The corn steep liquor yeast fermentation broth used in the Experimental Example was kept at a final concentration of 1 g / 100 mL.

Manufacturing example  2. Bifidobacterium  Production of Lactic Acid Fermentation Lactic Acid with Corn Bean

After washing with purified water and drying the corn hairs, they were pulverized and finely ground using 300 mesh. This was added to the culture solution of lactic acid bacteria to be 10 g / L. Glucose was added as a carbon source, and Dipotassium phosphate was added as a pH regulator. Bifidobacterium was used as a lactic acid bacterium and added at 50,000 cfu / L per culture. Cultures were cultured in a 5 L fermenter for 7 days at 37 ° C and pH 5-7. After culturing, the culture was centrifuged, and the culture was firstly removed and finally filtered using 0.25 μM filter paper. The filtrate thus obtained was aged for about 10 days at a low temperature warehouse at 4 DEG C, and the filtrate was filtered again using a 0.25 mu M filter and used in the present invention. The thus prepared fermentation broth is hereinafter referred to as " corn steep liquor fermentation broth. &Quot; The fermentation broth of corn steep liquor used in the experiments was maintained at a final concentration of 1 g / 100 ml.

Manufacturing example  3. Production of corn beard extract

The corn hairs were washed with purified water, dried and pulverized, and then 100 g was added to 0.6 L of a 70% ethanol solution. The mixture was heated and extracted with a reflux condenser equipped with a condenser for 4 hours. Then, the obtained extract was filtered with 300 mesh filter cloth, filtered through filter paper No. 5, and then the solvent was removed using a vacuum concentrator and lyophilized to obtain 5.5 g of dry weight. The extract thus obtained is hereinafter referred to as "corn hairs extract", and corn hairs extract is used at a final concentration of 1 g / 100 mL.

Experimental Example  1: Inhibition of tyrosinase activity of corn steep liquor

As a substrate, 40 μl of 1.5 mmol / l tyrosine (Sigma, USA) dissolved in sodium phosphate buffer (pH 6.8) was used. The fermented maize hawthorn fermentation broth prepared in Preparation Example 1 and the fermented maize hawthorn fermented broth prepared in Preparation Example 2 were each diluted with a 0.05 M sodium phosphate buffer solution (pH 6.8) to prepare a sample for each concentration (0.5% and 1.0%) . To the solution was added 20 μl of tyrosinase (Sigma, 1500 U / ml), and the mixture was reacted at 37 ° C. for 15 minutes. Then, the mixture was reacted at 490 nm Absorbance was measured. As a comparative group, the corn mustow extract of Preparation Example 3 was used in place of the corn steep liquor. As a control group, corn steep liquor without treatment was used.

Activity inhibition of tyrosinase was calculated by the following formula (1). The experimental results are shown in Table 1 below.

division Test substance concentration (%) Inhibition rate of tyrosinase activity (%) Production Example 1 0.5% 44.7 1.0% 62.6 Production Example 2 0.5% 53.9 1.0% 74.8 Production Example 3 0.5% 31.6 1.0% 45.8

From the results, it was found that the treatment with corn steep liquor yeast fermentation broth and corn steep liquor fermentation broth was superior to the corn steep liquor extract (comparative group) in inhibiting tyrosinase activity. Further, the fermentation of corn steep liquor inhibited the production of melanin Skin pigment problem could be solved.

Experimental Example  2: Melanin biosynthesis inhibition experiment of corn steep liquor

B-16 cells (mouse melanoma cells and Korean cell line bank) were inoculated on a 6-well plate at 1 × 10 ⁵ cells / well and cultured for 24 hours. Each well was treated with 0.5% and 1% corn steep liquor, respectively, for 3 to 4 days, and the culture broth of each well was centrifuged. The separated cells were dissolved in 500 μl of 1N NaOH solution in dimethylsulfoxide (DMSO) and heated in a 90 ° C water bath for 10 minutes. The absorbance of this supernatant was measured at 490 nm. As a control group, corn steep liquor without treatment was used. The inhibition rate of melanin production was calculated according to the following formula (2), and the results are shown in Table 2.

division Test substance concentration (%) Melanin production inhibition rate (%) Production Example 1 0.5% 34.8 1.0% 79.1 Production Example 2 0.5% 46.5 1.0% 85.9 Production Example 3 0.5% 35.6 1.0% 56.6

The results showed that the treatment with corn steep liquor yeast fermentation broth and corn steep liquor fermentation broth was superior to the corn steep liquor extract (comparative group) in inhibiting tyrosinase activity and inhibiting melanin biosynthesis, It was found that the skin pigment problem can be solved by inhibiting melanin production. Hereinafter, a cosmetic composition containing the fermentation broth of corn steep liquor according to the present invention is prepared and presented based on the results of the above experimental examples. However, the composition of the present invention is not intended to be limited to the following formulations. Any of corn steep liquor yeast fermentation broth or corn steep liquor fermentation broth may be used as the corn steep liquor fermentation solution used in the following prescription examples.

Formulation Example  1: softening longevity

Examples of prescription of softening longevity in cosmetics containing corn steep liquor fermentation liquid are as follows.

ingredient Content (% by weight) Corn barley fermentation broth 0.5 glycerin 5.0 1,3-butylene glycol 3.0 Allantoin 0.1 DL-Panthenol 0.3 Lee D. TEE-2NA 0.02 Benzophenone-9 0.04 Sodium hyaruronate 5.0 Nicole HCO 60 0.4 Methyl paraben 0.2 incense, 0.01 Distilled water Balance Sum 100

Formulation Example  2: Nourishing lotion

Examples of prescription of nutritional lotion among cosmetics containing corn steep liquor fermentation liquid are as follows.

ingredient Content (% by weight) Corn barley fermentation broth 2.0 Glyceryl stearate SE 1.5 Stearyl alcohol 1.5 lanolin 1.5 Polysorbate 60 1.3 Sorbitan stearate 0.5 Hardened vegetable oil 1.0 Mineral oil 5.0 Squalane 3.0 Trioctanoin 2.0 Dimethicone 0.8 Tocopheryl acetate 0.5 Carboxyvinyl polymer 0.12 glycerin 5.0 1,3-butylene glycol 3.0 Sodium hyaruronate 5.0 Triethanolamine 0.12 Methyl paraben 0.2 incense 0.02 Distilled water Balance Sum 100

Formulation Example  3: Nourishing cream

Examples of prescription of nutritional cream among cosmetics containing corn barley fermentation liquid are as follows.

ingredient Content (% by weight) Corn barley fermentation broth 3.0 Pro-type glycerin monostearate 2.0 Cetearyl alcohol 2.2 Stearic acid 1.5 Wax 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Hardened vegetable oil 1.0 Squalane 3.0 Mineral oil 5.0 Trioctanoin 5.0 Dimethicone 1.0 Sodium magnesium silicate 0.1 glycerin 5.0 Betaine 3.0 Triethanolamine 1.0 Sodium hyaruronate 4.0 Methyl paraben 0.2 incense 0.05 Distilled water Balance Sum 100

Experimental Example  3: Cosmetics  Experiment of whitening effect of composition

The whitening effect test of the cosmetic composition of the present invention was conducted on 40 Korean women of 20 to 40 years old, especially skinned black. A product (Example 1) containing 3% corn steep liquor yeast fermentation broth and 3% cornstarch fermented broth fermentation broth (Example 2) were prepared as described in Formulation Example 3, and as Comparative Example 1, corn steep liquor fermentation broth , And a product containing 3% corn salad extract as Comparative Example 2 was prepared. In the experiment, first, 20 patients used both sides of the face for the control product and the product containing the fermented yeast fermented with corn harts. Twenty subjects used the products containing the comparative product and the fermented product of corn hairs lactobacillus, Were used. Minolta CR 300 was used for the measurement and it was measured for 8 weeks. The experimental results were compared with the L value taken at 8 weeks and the L value taken at 0 week. The results are shown in Table 8 below.

division Comparative Example 1 Comparative Example 2 Example 1 Example 2 L value 0.125 0.235 0.453 0.468

As shown in Table 6, the products containing corn steep liquor (Examples 1 and 2) showed higher whitening effect than Comparative Example 1, which did not contain corn hog extract. In addition, it showed much higher whitening effect than Comparative Example 2 containing corn beard extract. In other words, the whitening effect is increased by fermenting the cornstarch extract with whitening activity. Therefore, it can be seen that the cosmetic composition of the present invention has an excellent whitening effect.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (5)

A cosmetic composition for skin whitening comprising a corn steep liquor obtained by culturing a corn beard with yeast or lactic acid bacteria. [Claim 2] The method according to claim 1, wherein the corn steep liquor is a fermentation liquor obtained by cultivating corn steat using Saccharomyces cerevisiae as a yeast or Bifidobacterium as a lactic acid bacterium A cosmetic composition for skin whitening. The cosmetic composition for whitening skin according to claim 1, wherein the fermented corn steep liquor is contained in an amount of 0.0001 to 30.0% by weight based on the total weight of the cosmetic composition. The cosmetic composition for skin whitening according to claim 1, wherein the fermented corn steep liquor inhibits melanin production and inhibits tyrosinase activity. The cosmetic composition according to claim 1, wherein the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and spray Lt; RTI ID = 0.0 > cosmetic < / RTI > composition for skin whitening.