JP5461832B2 - Plant extract-containing composition - Google Patents
Plant extract-containing composition Download PDFInfo
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- JP5461832B2 JP5461832B2 JP2008323281A JP2008323281A JP5461832B2 JP 5461832 B2 JP5461832 B2 JP 5461832B2 JP 2008323281 A JP2008323281 A JP 2008323281A JP 2008323281 A JP2008323281 A JP 2008323281A JP 5461832 B2 JP5461832 B2 JP 5461832B2
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Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Description
本発明は、ヒメウィキョウ、グリーンオーツ、ギシギシ、ラケモサ、チャボトケイソウからなる群から選ばれる一種または二種以上の植物抽出物及びクミスクチン抽出物を含有する植物抽出物含有組成物に関し、更に詳しくは、線維芽細胞増殖因子５（以下、「ＦＧＦ−５」と略する。）遺伝子の発現抑制効果を有する植物抽出物含有組成物に関する。 The present invention relates to a plant extract-containing composition containing one or more kinds of plant extracts selected from the group consisting of Himewikyo, Green Oats, Swordfish, Rakemosa, Chabotoiso, and a kumistin extract. The present invention relates to a plant extract-containing composition having an effect of suppressing the expression of blast growth factor 5 (hereinafter abbreviated as “FGF-5”) gene.
ＦＧＦ−５は線維芽細胞の増殖活性を有する因子のひとつであり、近年、神経細胞、血管内皮細胞、軟骨細胞や骨芽細胞の増殖・分化促進または抑制効果、血小板や発毛・育毛の調節作用も有することが知られてきた。そこで、ＦＧＦ−５の産生に関与するＦＧＦ−５遺伝子の発現が調節できれば、皮膚組織、循環器系組織、骨格系組織や毛髪に対して種々の効果が期待できる。（特許文献１） FGF-5 is one of the factors having fibroblast proliferation activity. In recent years, FGF-5 promotes proliferation / differentiation of nerve cells, vascular endothelial cells, chondrocytes and osteoblasts, and regulates platelets, hair growth and hair growth. It has also been known to have an effect. Thus, if the expression of the FGF-5 gene involved in the production of FGF-5 can be regulated, various effects can be expected on skin tissue, circulatory system tissue, skeletal system tissue and hair. (Patent Document 1)
ヒメウィキョウ、グリーンオーツ、ギシギシ、ラケモサ、チャボトケイソウ、クミスクチンは、健康増進や美容の目的で茶などの形態で食用にされている植物であるが、ＦＧＦ−５遺伝子の発現を抑制する効果があることは知られていない。
本発明は、クミスクチン抽出物とヒメウィキョウなどから選ばれる特定の植物抽出物を配合した優れたＦＧＦ−５遺伝子の発現を抑制する作用を有する植物抽出物含有組成物を提供することを目的とする。 An object of this invention is to provide the plant extract containing composition which has the effect | action which suppresses the expression of the outstanding FGF-5 gene which mix | blended the specific plant extract chosen from a cumistin extract, Himewikyo, etc.
本発明者らは、鋭意検討したところヒメウィキョウ、グリーンオーツ、ギシギシ、ラケモサ、チャボトケイソウからなる群から選ばれる一種または二種以上の植物抽出物及びクミスクチン抽出物を組み合わせて配合した植物抽出物含有組成物に優れたＦＧＦ−５遺伝子発現抑制作用があることを見出し、本発明を完成させた。 The inventors of the present invention have intensively studied, and include a plant extract-containing composition that is a combination of one or more plant extracts selected from the group consisting of Himewikyo, Green Oats, Gishigishi, Rakemosa, Chabottoiso, and a kumistin extract. The present invention was completed by finding that the product has an excellent FGF-5 gene expression inhibitory effect.
すなわち、本発明は下記の植物抽出物含有組成物を提供するものである。
項１. ヒメウィキョウ、グリーンオーツ、ギシギシ、ラケモサ、チャボトケイソウからなる群から選ばれる一種または二種以上の植物抽出物及びクミスクチン抽出物を含有することを特徴とする植物抽出物含有組成物。
項２. 前記植物抽出物の抽出溶媒が、水、エタノール、あるいは含水エタノールの何れかであることを特徴とする項１記載の組成物。
項３. クミスクチン抽出物の抽出溶媒が、水、メタノール、エタノールまたはそれらの混合溶媒の何れかであることを特徴とする項１記載の植物抽出物含有組成物。
項４. 皮膚外用剤である項１〜３の何れかに記載の植物抽出物含有組成物。
項５.経口組成物である項１〜３の何れかに記載の植物抽出物含有組成物。
That is, the present invention provides the following plant extract-containing composition.
Item 1. A plant extract-containing composition comprising one or two or more plant extracts selected from the group consisting of Himewikyo, Green Oats, Gishigishi, Rakemosa, Chabottoiso, and a cumistin extract.
Item 2. The composition according to Item 1, wherein an extraction solvent of the plant extract is water, ethanol, or water-containing ethanol.
Item 3. The plant extract-containing composition according to Item 1, wherein the extraction solvent of the cumistin extract is water, methanol, ethanol, or a mixed solvent thereof.
Item 4. The plant extract-containing composition according to any one of Items 1 to 3, which is an external preparation for skin.
Item 5. The plant extract-containing composition according to any one of Items 1 to 3, which is an oral composition.
本発明によれば、優れたＦＧＦ−５遺伝子発現抑制作用を有する植物抽出物含有組成物が提供され、そのＦＧＦ−５遺伝子の発現を抑制する作用により、脱毛予防効果、心疾患予防効果、骨代謝改善効果が期待できる。 ADVANTAGE OF THE INVENTION According to this invention, the plant extract containing composition which has the outstanding FGF-5 gene expression inhibitory effect is provided, By the effect | action which suppresses the expression of the FGF-5 gene, a hair loss prevention effect, a heart disease prevention effect, bone Metabolic improvement effect can be expected.
以下に本発明を詳細に説明する。 The present invention is described in detail below.
本発明に用いるヒメウィキョウ抽出物は、西アジア原産のセリ科の多年草植物であるヒメウィキョウの葉、茎部、果実、種子または地上部の抽出物であり、特に果実、種子の抽出物が好適である。 The extract of Himewikyo used in the present invention is an extract of the leaves, stems, fruits, seeds or aerial parts of Himewikyo, which is a perennial plant belonging to the family Apiaceae native to West Asia, and fruit and seed extracts are particularly preferred. .
本発明に用いるグリーンオーツ抽出物は、別名エンバクまたはオーツ麦と呼ばれる、ユーラシア大陸原産のイネ科植物であるグリーンオーツの葉、茎部、種子または地上部の抽出物であり、特に緑色を呈する地上部の抽出物が好適である。 The green oat extract used in the present invention is an extract of leaves, stems, seeds or aboveground parts of green oats, which is a gramineous plant native to the Eurasian continent, also called oats or oats. Part of the extract is preferred.
本発明に用いるギシギシ抽出物は、日本や朝鮮半島に分布するタデ科多年草植物であるギシギシ、ナカバギシギシ、アレチギシギシの葉、茎部、果実部、地上部または根部の抽出物であり、特に葉部や茎部の抽出物が好ましい。 Gishigishi extract used in the present invention is an extract of leaves, stems, fruit parts, above-ground parts or root parts of gypsidae, Nakabagishigishi, Arechigishigishi, which is a perennial perennial plant distributed in Japan and the Korean peninsula, especially leaf parts. An extract of stalk and stem is preferred.
本発明に用いるラケモサ抽出物は、別名ブラックコホシュと呼ばれ、北米原産のキンポウゲ科多年草植物であるラケモサの葉、茎部または地上部の抽出物である。 The Lachemosa extract used in the present invention is also called black cohosh, and is an extract of Lachemosa leaves, stems or above-ground parts, which are North American buttercup perennial plants.
本発明に用いるチャボトケイソウ抽出物は、別名パッションフラワーと呼ばれる、中央アメリカ原産のトケイソウ科蔓性多年草植物であるチャボトケソウの葉、茎部、花部、種子または地上部の抽出物であり、特に地上部の抽出物が好ましい。 The Chabotodia extract used in the present invention is an extract of the leaves, stems, flower parts, seeds or aboveground parts of the Cloveraceae genus perennial plant native to Central America, also known as Passion Flower, especially on the ground. Part of the extract is preferred.
本発明に用いるクミスクチン抽出物は、別名ネコノヒゲと呼ばれる、インド、マレーシア原産のシソ科多年草植物であるクミスクチンの葉、茎部または地上部の抽出物であり、特に地上部の抽出物が好ましい。 The cumistin extract used in the present invention is an extract of leaves, stems or aboveground parts of cumisctin, which is a perennial plant of Lamiaceae native to India and Malaysia, also known as Nekonohige, and the above ground part extract is particularly preferable.
本発明に用いる植物抽出物およびクミスクチン抽出物は、常法により製造でき、抽出溶媒の種類や使用量、抽出温度、抽出時間等は、適宜設定することができる。 The plant extract and cumistin extract used in the present invention can be produced by a conventional method, and the type and amount of the extraction solvent, the extraction temperature, the extraction time and the like can be appropriately set.
抽出溶媒としては、水、エチルアルコール、石油エーテル、ヘキサン、ブタノール、プロパノール、メタノール、ポリエチレングリコール、プロピレングリコール、ブチレングリコールなどが挙げられ、このうち、水、エタノールなどの極性溶媒が好ましく、特に水、エタノール及び含水エタノール好ましい。 Examples of the extraction solvent include water, ethyl alcohol, petroleum ether, hexane, butanol, propanol, methanol, polyethylene glycol, propylene glycol, butylene glycol, etc. Among these, polar solvents such as water and ethanol are preferable, particularly water, Ethanol and hydrous ethanol are preferred.
これら植物抽出物は、更に濃縮、スプレードライ、凍結乾燥、精製などの処理することができる。 These plant extracts can be further processed for concentration, spray drying, freeze drying, purification and the like.
本発明では、ヒメウィキョウ、グリーンオーツ、ギシギシ、ラケモサ、チャボトケイソウの抽出物から単独もしくは二種以上を選択して、配合でき、それらとクミスクチン抽出物との配合比率は、任意に設定できる。 In this invention, it can mix | blend individually or 2 or more types from the extract of Himewikyo, green oats, scallop, rakemosa, chabotoiso, and the mixing | blending ratio of them and a kumisctin extract can be set arbitrarily.
本発明の植物抽出物含有組成物における植物抽出物およびクミスクチン抽出物の含有量は、添加形態および投与形態によっても異なるが、広い範囲から選択できる。例えば、植物抽出物またはクミスクチン抽出物の配合量は、溶媒抽出乾燥物換算で、組成物中に０.０１〜６０重量％配合することができ、０.１〜２０重量％配合することが好ましい。また、植物抽出物およびクミスクチン抽出物の配合比率は、植物抽出物の合計量とクミスクチン抽出物の配合重量比率が１：１０〜１０：１が好ましく、５：１〜１：３がより好ましい。 The content of the plant extract and the cumistin extract in the plant extract-containing composition of the present invention varies depending on the addition form and the administration form, but can be selected from a wide range. For example, the blending amount of the plant extract or the cumistin extract can be blended in the composition in an amount of 0.01 to 60% by weight, preferably 0.1 to 20% by weight in terms of the solvent extract dry matter. . In addition, the blending ratio of the plant extract and the cumistin extract is preferably 1:10 to 10: 1, and more preferably 5: 1 to 1: 3, as the total weight of the plant extract and the cumistin extract.
本発明の植物抽出物含有組成物は、優れたＦＧＦ−５遺伝子発現抑制作用を有することから、育毛効果を奏する医薬部外品、医薬品、毛髪用化粧料、機能性食品等や心疾患を予防する医薬部外品、医薬品、機能性食品等として提供できる。その投与形態は、経口、あるいは外用の何れでもよいが、経口投与が好ましい。 Since the plant extract-containing composition of the present invention has an excellent FGF-5 gene expression inhibitory action, it prevents quasi-drugs, pharmaceuticals, cosmetics for hair, functional foods, etc. that have a hair-growth effect and heart disease. Can be provided as quasi-drugs, pharmaceuticals, functional foods, etc. The administration form may be either oral or external, but oral administration is preferred.
本発明の植物抽出物含有組成物を皮膚外用剤とする場合、頭皮に、効果的に塗布される態様が好ましく、例えば、液剤、エアゾール剤、ジェル剤などが挙げられ、育毛剤、ヘアトニック、ヘアジェル、ヘアクリーム、シャンプー、リンス、トリートメント、整髪剤等として提供できる。 When the plant extract-containing composition of the present invention is used as an external preparation for skin, an embodiment in which it is effectively applied to the scalp is preferable, and examples thereof include liquid agents, aerosol agents, gel agents, and the like. It can be provided as hair gel, hair cream, shampoo, rinse, treatment, hair conditioner, etc.
本発明の植物抽出物含有組成物を経口組成物とする場合、錠剤、顆粒、細粒、粉体、タブレット、カプセル、液体、ペースト、ゲルなどの態様、すなわち、粉末茶、サプリメント、トローチ、飲料などとして食品、機能性食品、特定保健用食品、病者用食品などに提供できる。 When the plant extract-containing composition of the present invention is used as an oral composition, embodiments such as tablets, granules, fine granules, powders, tablets, capsules, liquids, pastes, gels, that is, powdered tea, supplements, troches, beverages It can be provided as food, functional food, food for specified health use, food for the sick, etc.
本発明の植物抽出物含有組成物は、本発明の効果を損なわない範囲において、植物抽出物のほかに、食品、化粧品、医薬部外品、医薬品等に配合できる薬効剤、例えば、抗炎症剤、細胞不活化剤、皮脂分泌抑制剤、抹消血管拡張剤、アミノ酸類、ビタミン類等、精製水、エタノール、油性物質、保湿剤、増粘剤、防腐剤、乳化剤、薬効成分、粉体、紫外線吸収剤、色素、香料、乳化安定化剤等を任意に組み合わせて配合することができる。 The plant extract-containing composition of the present invention is a medicinal agent that can be added to foods, cosmetics, quasi drugs, pharmaceuticals, etc., in addition to the plant extract, as long as the effects of the present invention are not impaired. , Cell inactivators, sebum secretion inhibitors, peripheral vasodilators, amino acids, vitamins, etc., purified water, ethanol, oily substances, moisturizers, thickeners, preservatives, emulsifiers, medicinal ingredients, powders, ultraviolet rays Absorbers, pigments, fragrances, emulsion stabilizers and the like can be combined in any combination.
以下、試験例、実施例により本発明をより詳細に説明するが、本発明はこれらに限定されるものではない。 EXAMPLES Hereinafter, although this invention is demonstrated in detail by a test example and an Example, this invention is not limited to these.
＜試験例１；ＦＧＦ−５遺伝子の発現抑制効果＞
下記の植物抽出物を各濃度でクミスクチン抽出物と組み合わせた場合のＦＧＦ−５遺伝子の発現抑制効果を、ヒト毛乳頭細胞を用いて評価した。結果を、段落番号[００２９]に示す。
<Test Example 1; FGF-5 gene expression suppression effect>
The expression suppression effect of the FGF-5 gene when the following plant extract was combined with the cumistin extract at each concentration was evaluated using human hair papilla cells. The results are shown in paragraph number [0029].
評価に供したクミスクチン抽出物および植物抽出物
クミスクチン抽出物：地上部を熱水で抽出し、粉末化したもの。
チャボトケイソウ：地上部を６０％含水エタノールで抽出し、粉末化したもの。
ヒメウィキョウ：種子を含む果実を熱水で抽出し、粉末化したもの。
グリーンオーツ：緑色を呈する地上部を３０％含水エタノールで抽出し、粉末化したもの。
セイヨウカボチャ：種子を６０％含水アルコールで抽出し、粉末化したもの。
ギシギシ：乾燥葉部２.５ｇを250ｍｌの熱水で15分間抽出して得られた液体。
ラケモサ：乾燥葉部２.５ｇを250ｍｌの熱水で15分間抽出して得られた液体。
ニトベギク：全草を熱水で抽出し、粉末化したもの。
コーヒー豆：未焙煎のコーヒー豆を６０％含水アルコールで抽出し、粉末化したもの。
Cumistin extract and plant extract used for evaluation Cumistin extract: Extracted above ground with hot water and powdered.
Chabotodia: Extracted above ground with 60% water-containing ethanol and powdered.
Himewikyo: Fruits containing seeds extracted with hot water and powdered.
Green oats: The above-ground part showing green is extracted with 30% water-containing ethanol and powdered.
Pumpkin: A seed extracted from 60% hydrous alcohol and powdered.
Gishigishi: A liquid obtained by extracting 2.5 g of dried leaves with 250 ml of hot water for 15 minutes.
Lachemosa: A liquid obtained by extracting 2.5 g of dried leaves with 250 ml of hot water for 15 minutes.
Nitobegiku: Whole plant extracted with hot water and powdered.
Coffee beans: Unroasted coffee beans extracted with 60% hydrous alcohol and powdered.
試験方法
ＰＣＧＭを用いてヒト毛乳頭細胞をサブコンフルエントになるまで培養した。培養した細胞に試験サンプルを添加し、１％ＦＢＳ−ＤＭＥＭ培地でさらに２４時間培養した後、逆転写ポリメラーゼ連鎖反応法を用いてＦＧＦ−５遺伝子の発現量を測定した。得られた測定値は下記の数１にしたがって換算し、クミスクチン抽出物のＦＧＦ−５遺伝子発現抑制効果の増強率を求めた。
試験サンプルは、クミスクチン抽出物（測定時濃度 １０μｇ/ｍｌ）と前記の植物抽出物を各濃度（測定時濃度）で組み合わせたものを用いた。また、ネガティブコントロールは蒸留水を、ポジティブコントロールはクミスクチン抽出物（測定時濃度 １０μｇ/ｍｌ）を用いた。
Test method Human hair papilla cells were cultured to sub-confluence using PCGM. A test sample was added to the cultured cells, and the cells were further cultured in 1% FBS-DMEM medium for 24 hours, and then the expression level of the FGF-5 gene was measured using a reverse transcription polymerase chain reaction method. The obtained measured value was converted according to the following formula 1, and the enhancement rate of the FGF-5 gene expression inhibitory effect of the cumistin extract was determined.
The test sample used was a combination of the cumistin extract (measurement concentration 10 μg / ml) and the above plant extract at each concentration (measurement concentration). Further, distilled water was used as a negative control, and a cumistin extract (concentration at measurement: 10 μg / ml) was used as a positive control.
Ｂ：ポジティブコントロール
Ｃ：試験サンプル
以上の試験により、ヒメウィキョウ、グリーンオーツ、ギシギシ、ラケモサ、チャボトケイソウの各抽出物とクミスクチン抽出物を併用することで優れたＦＧＦ−５遺伝子の発現抑制効果が得られることが判明した。すなわち、クミスクチン抽出物とヒメウィキョウ、グリーンオーツ、ギシギシ、ラケモサ、チャボトケイソウの各抽出物を組合わせて配合した植物抽出物含有組成物は、優れた脱毛予防効果、抗癌効果や心筋梗塞予防効果を有することが期待できる。 From the above test, it was found that the excellent FGF-5 gene expression-suppressing effect can be obtained by using each of the extract of hime fennel, green oats, scallop, rakemosa, chabotodia and cumistin extract. In other words, a plant extract-containing composition that is a combination of a Kumisctin extract and each of the extracts of Himewikyo, Green Oats, Gishigishi, Rakemosa, Chabotoiso, has an excellent hair loss prevention effect, anticancer effect and myocardial infarction prevention effect. You can expect to have.
下記原料を使用して常法により植物抽出物含有組成物を製造した。なお、（％）は（重量％）を意味する。 The plant extract containing composition was manufactured by the conventional method using the following raw material. Note that (%) means (% by weight).
実施例１
常法に従い、下記の顆粒状食品を製造した。
According to a conventional method, the following granular food was produced.
実施例２
常法に従い、下記の粉末茶を製造した。
The following powdered tea was produced according to a conventional method.
実施例３
常法に従い、下記のタブレットを製造した。
The following tablets were produced according to a conventional method.
実施例４
常法に従い、下記のタブレットを製造した。
The following tablets were produced according to a conventional method.
実施例５
常法に従い、下記の飲料を製造した。
According to a conventional method, the following beverages were produced.
実施例６
常法に従い、下記の頭皮用ローションを製造した。
According to a conventional method, the following scalp lotion was produced.
実施例７
常法に従い、下記の育毛剤を製造した。
According to a conventional method, the following hair restorer was produced.
実施例８
常法に従い、下記のヘアトニックを製造した。
According to a conventional method, the following hair tonic was produced.
実施例９
常法に従い、下記のヘアジェルを製造した。
According to a conventional method, the following hair gel was produced.
Claims (4)
（Ａ）の合計配合量（乾燥物換算）が組成物全体に対して０．００５〜１重量％
（Ｂ）の配合量（乾燥物換算）が組成物全体に対して０．００１〜０．５重量％
であることを特徴とする植物抽出物を含有するＦＧＦ−遺伝子発現抑制剤。 (A) One or two or more kinds of plant extracts selected from the group consisting of Himewikyo and Green Oats, and (B) Cumistin extract, and (A) total blended amount (dry matter equivalent) is the whole composition 0.005 to 1% by weight with respect to
The blending amount of (B) (in terms of dry matter) is 0.001 to 0.5% by weight based on the entire composition.
An FGF-gene expression inhibitor containing a plant extract characterized by
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| JP5390179B2 (en) * | 2008-12-24 | 2014-01-15 | 花王株式会社 | IGFBP-5 expression inhibitor |
| JP6257876B2 (en) * | 2010-07-02 | 2018-01-10 | 花王株式会社 | VEGF production promoter |
| KR101184184B1 (en) | 2012-05-29 | 2012-09-18 | 노세응 | Hair growth stimulants and the method thereof |
| JP5828355B1 (en) * | 2014-09-22 | 2015-12-02 | 和伎獅子株式会社 | Powdered skin external preparation for hair growth / hair growth and skin condition improvement |
| KR102274305B1 (en) * | 2014-11-18 | 2021-07-08 | (주)아모레퍼시픽 | Composition for improving scalp condition comprising flower extract of passiflora laurifolia |
| KR102299277B1 (en) * | 2014-11-18 | 2021-09-08 | (주)아모레퍼시픽 | Composition for improving scalp condition comprising flower extract of passiflora edulis |
| KR102249708B1 (en) * | 2014-11-18 | 2021-05-10 | (주)아모레퍼시픽 | Composition for improving scalp condition comprising flower extract of passiflora quadrangularis |
| JP2019196327A (en) * | 2018-05-09 | 2019-11-14 | 森永製菓株式会社 | Hair growth promoter, medicine, food and cosmetic containing extract of passion fruit seeds as active ingredient |
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| JP3316938B2 (en) * | 1993-06-02 | 2002-08-19 | 日本油脂株式会社 | Production method of Rumex japonicus extract |
| JPH1029924A (en) * | 1996-07-12 | 1998-02-03 | Shiseido Co Ltd | Antiaging agent |
| JP2000026228A (en) * | 1998-07-10 | 2000-01-25 | Nippon Flour Mills Co Ltd | Collagenase inhibitor, moisturizer, and cosmetic or food containing the same |
| JP2000095663A (en) * | 1998-09-24 | 2000-04-04 | Kose Corp | Agent for external use containing plant extract |
| JP2001122757A (en) * | 1999-10-22 | 2001-05-08 | Naris Cosmetics Co Ltd | Active oxygen scavenger, antioxidant and cosmetic |
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| JP4542300B2 (en) * | 2002-02-15 | 2010-09-08 | 株式会社ファンケル | Hyaluronic acid accumulation promoter |
| JP2005272399A (en) * | 2004-03-25 | 2005-10-06 | Kao Corp | Hair cosmetic |
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| JP2007238509A (en) * | 2006-03-09 | 2007-09-20 | Asahi Breweries Ltd | Agent for ameliorating skin symptom, supplement, food, beverage and pharmaceutical for ameliorating skin symptom containing the same |
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