JP5062980B2 - Moisturizer, collagen production promoter, whitening agent, antioxidant, and vascular endothelial growth factor production promoter - Google Patents

Description

  The present invention relates to a humectant, a collagen production promoter, a whitening agent, an antioxidant, a vascular endothelial growth factor production promoter, a skin external preparation, and a cosmetic food. More specifically, the present invention relates to a moisturizer, collagen production promoter, whitening agent, antioxidant, vascular endothelial growth factor production promoter, skin external preparation, hair restorer, and beauty food containing an extract of the plant belonging to the genus Amber.

  Causes of skin symptoms such as aging, ultraviolet rays, stress, wrinkles, stains, skin elasticity decrease, dryness, decreased cellular function, melanin production and pigmentation due to ultraviolet rays, decrease or degeneration of dermal matrix components, ultraviolet rays, etc. Examples include oxidative damage. In order to prevent and ameliorate such skin symptoms, search for various active ingredients and formulation studies have been conventionally conducted. As the collagen production promoter, the essence of Ponkan (see Patent Document 1), as the whitening agent, water and / or organic solvent extract of Hakutsuru Reishi (see Patent Document 2), and as the antioxidant, Salogaseaceae An extract of a genus plant (see Patent Document 3) is known.

  In addition, the prior art regarding a moisturizer, a collagen production promoter, a whitening agent, an antioxidant, an external preparation for skin, and a food containing an extract of the plant of the genus Halibut as an active ingredient was not recognized.

JP 2001-131045 A JP 2003-89630 A Japanese Patent Laid-Open No. 10-182413

  Naturally-derived components are known to have various pharmacological and cosmetic effects, and so far many plants and fungi have been widely applied to fields such as external preparations for skin and foods and drinks. However, there are many naturally-derived ingredients whose effects are not yet known, and the development of effective ingredients having excellent collagen production promoting action, whitening action, antioxidant action and the like has been expected. The present invention was made in order to find such an active ingredient, and is a moisturizer, collagen production promoter, whitening agent, antioxidant, blood vessel that can be widely applied in the fields of external preparations for skin and foods and drinks. An object of the present invention is to provide an endothelial cell growth factor production promoter.

  In order to find moisturizers, collagen production promoters, whitening agents, and antioxidants that can be widely applied to fields such as external preparations for skin and foods and beverages, the present inventors have examined various naturally-derived substances. went. As a result, they have found excellent moisturizing action, collagen production promoting action, whitening action, antioxidant action, and vascular endothelial growth factor production promoting action, and further studies have been made to complete the present invention. It was. That is, the present invention relates to a moisturizer, collagen production promoter, whitening agent, antioxidant, vascular endothelial growth factor production promoter, and one or two or more plants of American Caribbean An external preparation for skin, a hair-restoring agent and a food containing the above extract are provided.

  According to the present invention, it is possible to provide a moisturizer, a collagen production promoter, a whitening agent, an antioxidant, and a vascular endothelial growth factor production promoter having an excellent effect, and these can be used as a skin external preparation, food, etc. By blending with this composition, it is possible to provide various compositions that exhibit an excellent effect in preventing and improving various skin symptoms such as wrinkles, tarmi, skin firmness, spots, and kusumi.

The plant belonging to the genus Amaranthus used as a raw material of the present invention is a plant belonging to the genus Asteraceae, which is a plant of a low leaf tree. The oplopanax japonicus plants of the genus, the United States oplopanax japonicus (Oplopanax horridus), Korean oplopanax japonicus (Oplopanax elatus), oplopanax japonicus (Oplopanax japonicus), Hirohaharibuki (Oplopanax japonicus var. Brevilobus) and the like are known. In the present invention, any of these plants belonging to the genus Halibut may be used, but from the viewpoint of effectiveness and usability, it is preferable to use American Amaranthus or Amaranthus, especially American Amaranthus.

  In the present invention, a pulverized plant can be used, but an extract may be used from the viewpoint of usability. For extraction, any part of the stems, leaves, flowers, seeds, roots, buds, fruits, etc. of the plant of the genus Amaranth can be used. From the standpoint of effectiveness, stems and roots should be used. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days.

  Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, ethers such as ethyl ether and propyl ether. And solvents such as esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  The extract of the plant of the genus Amaranth can be used as it is, but the concentrated and dried product can be used again by dissolving it in water or a polar solvent, and the physiological effects thereof are not impaired. It may be used after performing purification treatment such as decolorization, deodorization and desalting, and fractionation treatment by column chromatography. The above-mentioned extract of Amaranthus or its processed products and fractions can be freeze-dried after each processing and fractionation and dissolved in a solvent at the time of use.

  An extract of the plant of the genus Halibut has excellent moisturizing action, collagen production promoting action, whitening action, antioxidant action, and vascular endothelial growth factor production promoting action, moisturizing agent, collagen production promoting agent, whitening agent, It can be used as an oxidizing agent, a vascular endothelial growth factor production promoter, a skin external preparation, a hair restorer, and a cosmetic food. In addition, moisturizers, collagen production promoters, whitening agents, antioxidants, and vascular endothelial growth factor production promoters containing extracts from the plant of the genus Amber are used not only for the skin but also for the hair. And can be taken orally, and can be applied to foods, beverages, pharmaceuticals, and the like.

  A moisturizing agent containing an extract of the plant of the genus Amaranth as an active ingredient exhibits an excellent moisturizing effect on the skin and hair, and has a particularly high moisturizing effect on the skin.

  A collagen production promoter comprising an extract of the plant of the genus Achillea as an active ingredient exhibits an excellent effect in promoting the production of dermal matrix components, and in particular, exhibits an excellent effect in promoting the production of collagen.

  A whitening agent containing an extract of the plant of the genus Amaranthus as an active ingredient is effective for improving pigmentation symptoms such as spots and freckles, and is particularly effective for suppressing melanin production based on inhibition of tyrosinase activity.

  Antioxidants containing an extract of the plant of the genus Achillea as an active ingredient exhibit an excellent antioxidant effect, but exhibit an especially excellent effect on scavenging free radicals.

  Vascular endothelial growth factor production promoter, which uses an extract of Achillea spp. As an active ingredient, has excellent vascular endothelial growth factor production promoting action, and is expected to have effects such as wound healing, skin color improvement, hair growth and hair restoration it can.

  By blending extracts of the plant of the genus Amaranthus into various compositions, the prevention and improvement of skin symptoms such as wrinkles, tarmi, skin firmness, spots, kumi, dryness, fine lines, wounds, skin color deterioration, etc., or hair growth effect And a composition that exhibits a hair nourishing effect can be obtained. Examples of these compositions include an external preparation for skin, a hair restorer, a hair nourishing agent, and a food. If it is a skin external preparation, it can be used as a skin external preparation for moisturization, a skin external preparation for improving anti-aging, or a skin external preparation for whitening, based on the above-mentioned effects of the extract of the plant belonging to the genus Halibus. It can also be used as a hair restorer or hair nourishing agent. Furthermore, the extract of the plant belonging to the genus Halibut can be used for foods and beverages for the purpose of beauty, health maintenance or nutritional supplementation, but is suitable for use as a cosmetic food including beverages. .

  The blending amount of the extract of the plant belonging to the genus Amaranth can be adjusted depending on the type of composition and the purpose of use, etc. from the viewpoint of the effect and stability. The amount is preferably 0.0001 to 50.0% by weight, more preferably 0.001 to 25.0% by weight, based on the total amount.

  The dosage form of the composition containing the extract of the plant belonging to the genus Amaranth is arbitrary, but in the case of an external preparation for skin, a solubilizing system such as lotion, an emulsifying system such as cream or emulsion, a dispersion system such as calamine lotion, an aerosol It can also be provided in various dosage forms such as ointments, powders and granules. When the composition is an oral medicine or food, it should be a general dosage form such as a liquid preparation such as a drink or infusion, a solid preparation such as gum or candy, a capsule, powder, granule or tablet. Can do.

  In addition to the extract of the plant of the genus Halibus, the composition to which the extract of the plant of the genus Amaranth is added, if necessary, usually a pharmaceutical, a quasi-drug, a skin cosmetic, a cosmetic for hair, a cleaning agent. And oil ingredients, powders, pigments, emulsifiers, solubilizers, detergents, UV absorbers, thickeners, drugs, fragrances, resins, antibacterial and antifungal agents, alcohols, seasonings Further, excipients and the like can be appropriately blended. In addition, other moisturizers, collagen production promoters, whitening agents, antioxidants, and vascular endothelial growth factor production promoters can be used as long as the effects of the present invention are not impaired.

  In the following, the production examples of extracts of the plant belonging to the genus Halibut, the tests for evaluating each action, the preparation examples of skin external preparations and foods, the use tests will be explained in more detail, but the technical scope of the present invention It is not limited at all.

[Production Example 1]
10 kg of a 50 wt% aqueous ethanol solution was added to 1 kg of dried pulverized stems and roots of a genus Amaranthus plant and immersed for 7 days at room temperature. The extract was collected by filtration and the extract was obtained after removing the solvent.

[Production Example 2]
Nine liters of water was added to 1 kg of dried pulverized stems and roots of the plant belonging to the genus Amaranth, and the mixture was extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration, and after removing the solvent, an extract of the plant belonging to the genus Achillea was obtained.

[Production Example 3]
Nine liters of methanol was added to 1 kg of dried pulverized stems and roots of a genus Amaranthus and soaked at room temperature for 7 days. The extract was collected by filtration, and after removing the solvent, an extract of the plant belonging to the genus Achillea was obtained.

[Production Example 4]
A dried product of stems and roots of the plant of the genus Amaranth was introduced into a supercritical extraction apparatus, and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under a pressure of 15 MPa. The extract was collected to obtain an extract of the plant belonging to the genus Amaranthus.

  First, the collagen production promoting effect of an extract of the plant belonging to the genus Amber is shown. Samples obtained by extracting stems and roots of American red berries according to Production Example 4 were evaluated as Samples 1 and 2, respectively.

The evaluation was performed according to the following procedure. Normal human dermal fibroblasts are seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well, and 1.0 wt% fetal bovine serum-added Dulbecco's basal medium supplemented with an arbitrary concentration of sample ( DMEM) was cultured at 37 ° C. for 24 hours, and the amount of collagen in the culture supernatant was measured by ELISA. At the same time, the number of fibroblasts was counted, the amount of collagen produced per cell was calculated, and the relative amount of collagen produced per blank cell containing no sample as 100 was shown in Table 1. In addition, ** in the table represents a significance probability less than 1% (P <0.01) with respect to the significance probability P value in the t test.

  As can be seen from Table 1, in the medium supplemented with the American agate extract, a significant collagen production promoting action in dermal fibroblasts was observed. From this, it has been clarified that the extract of the plant belonging to the genus Achillea has an excellent collagen production promoting action.

  Next, it shows about the melanin production inhibitory effect of the extract of a genus Halibut. The samples were obtained by extracting the stems and roots of American red berries according to Production Example 4 as Samples 3 and 4, respectively, and those obtained by extracting the stems and roots of American red berries according to Production Example 1 as Samples 5 and 6, respectively. Went.

  The evaluation was performed according to the following procedure. B16 mouse melanoma F0 strain (B16F0) cells were seeded in a 35 mm dish at 2000 cells per dish. After culturing for 24 hours, the culture medium was replaced with Dulbecco's modified Eagle medium (DMEM) supplemented with 5 wt% fetal calf serum (FCS) to which a sample of an arbitrary concentration was added. After culturing for 7 days, the cells were collected using 0.25% trypsin, transferred to a 1.5 mL microtube, and centrifuged to obtain a cell precipitate. Furthermore, instead of the medium to which the sample was added, Dulbecco's modified Eagle medium (DMEM) to which 5 wt% fetal calf serum (FCS) was added was used as a blank, and visual determination was similarly performed. Further, as shown in Table 2, the visual judgment was performed by a five-step evaluation. Table 3 shows the determination results.

  As is clear from Table 3, an excellent revelation inhibitory effect on melanin was observed in the extract of Amaranthus. From this, it was clarified that the extract of the plant belonging to the genus Achillea has an excellent melanin production inhibitory action.

  Next, it shows about the antioxidant effect of the extract of a genus Halibut. Samples were obtained by extracting stems and roots of American red brilli according to Production Example 4 as Samples 7 and 8, respectively, and those obtained by extracting stems and roots of American Red brilli according to Production Example 1 as Samples 9 and 10, respectively. Went.

The evaluation was performed according to the following procedure. 100 μL of a sample diluted to an arbitrary concentration with a 50 wt% aqueous ethanol solution was added to a 96-well microplate. Next, 100 μL of a 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared in ethanol to a concentration of 0.2 mM was added to a 96-well microplate. The mixture was left in the dark with stirring, and the absorbance at 516 nm was measured after 24 hours. When the absorbance of the blank with no sample added was (A) and the absorbance when the sample was added was (B), the value of formula (1) was defined as the radical elimination rate. The evaluation results are shown in Table 4.
Formula (1) {1- (B) / (A)} × 100 (%)

  As can be seen from Table 4, an excellent repelling action was observed in the American agate extract. From this, it has been clarified that the extract of the plant belonging to the genus Amaranth has an antioxidant action based on radical scavenging.

  Next, an action of promoting the production of vascular endothelial growth factor by an extract of a plant belonging to the genus Achillea is shown. As samples, samples obtained by extracting stems and roots of American red berries according to Production Example 4 were used as Samples 11 and 12, respectively, and samples obtained by extracting American stems and roots of American Red biloba stems from Production Example 1 were evaluated as Samples 13 and 14, respectively. Went.

The evaluation was performed according to the following procedure. Normal human epidermal cells were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. As a seeding medium, commercially available Humdia-KG2 manufactured by Kurabo Industries, Ltd. was used. After culturing at 37 ° C. for 24 hours, the sample was replaced with a test medium added at an arbitrary concentration, further cultured for 24 hours, and the amount of vascular endothelial growth factor secreted into the culture supernatant was quantified by ELISA. In addition, Table 5 shows the relative values when a blank was used without adding a vascular endothelial growth factor production promoter and the blank vascular endothelial growth factor amount was 100.

  As is clear from Table 5, a significant vascular endothelial growth factor production-promoting effect was observed in the medium supplemented with Amaranthus extract. From this, it has been clarified that the extract of the plant belonging to the genus Amaranth has excellent vascular endothelial growth factor production promoting action.

  Then, the formulation example of a skin external preparation and a foodstuff is shown as a composition which mix | blended the extract of the plant of the genus Aberrant according to the present invention.

[Formulation Example 1] Emulsion (1) Squalane 10.0 (wt%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 53.85
(11) Arginine (1 wt% aqueous solution) 20.0
(12) American Caribbean extract [Production Example 1] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix uniformly.

[Prescription Example 2] Lotion (1) Ethanol 15.0 (% by weight)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 78.38
(5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) American Caribbean extract [Production Example 3] 5.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.

[Prescription Example 3] Cream (1) Squalane 10.0 (% by weight)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20% by weight aqueous solution) 15.0
(10) Purified water 36.7
(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) American Caribbean extract [Production Example 1] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification is completed, add (11), start cooling, add (12) at 40 ° C., and mix uniformly.

[Formulation Example 4] Cosmetic liquid (1) Purified water 27.45 (% by weight)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1 wt% aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by weight aqueous solution) 2.0
(16) American Caribbean extract [Production Example 1] 5.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C, add (16) and mix evenly.

[Formulation Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (% by weight)
(2) Purified water 78.7
(3) Sodium hydroxide (10% by weight aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) American Caribbean extract [Production Example 4] 10.0
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Formulation Example 6] Cleansing Fee (1) Squalane 77.0 (wt%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Purified water 3.0
(4) American Caribbean extract [Production Example 4] 5.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.

[Prescription Example 7] Face-wash foam (1) Stearic acid 16.0 (% by weight)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 31.5
(8) American Caribbean extract [Production Example 3] 6.0
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.

[Prescription Example 8] Make-up base cream (1) Squalane 10.2 (% by weight)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 65.4
(8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) American Caribbean extract [Production Example 1] 5.0
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Formulation Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (wt%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Purified water 53.4
(11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) American Caribbean extract [Production Example 4] 5.0
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Formulation Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) American Caribbean extract [Production Example 1] 5.0
(11) Purified water 43.4
(12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. while stirring and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.

[Prescription Example 11] Pack (1) Purified water 58.9 (% by weight)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 5.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) American Caribbean extract [Production Example 2] 5.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.

[Prescription Example 12] Bath agent (1) Fragrance 0.3 (% by weight)
(2) American Caribbean extract [Production Example 4] 5.0
(3) Sodium bicarbonate 46.0
(4) Sodium sulfate 48.7
Production method: (1) to (4) are mixed uniformly.

[Prescription Example 13] Hair wax (1) Stearic acid 3.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 70.6
(11) American Caribbean extract [Production Example 1] 5.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Prescription Example 14] Hair artic (1) Ethanol 46.0 (% by weight)
(2) Purified water 48.9
(3) American Caribbean extract [Production Example 3] 5.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

[Prescription Example 15] Beverage (1) American Caribbean extract [Production Example 1] 8.0 (wt%)
(2) Erythritol 1.0
(3) Citric acid 0.1
(4) Stevia 0.01
(5) Purified water 90.89
Production method: (1) to (5) are mixed uniformly.

  Next, a use test was conducted using a prescription formulated with an Amaranth extract, and the improvement effect was evaluated for rough skin caused by drying. At that time, each of the Amaranthus extracts listed in Table 6 was blended with the emulsion formulation shown in Formulation Example 1, and tests were conducted as Examples 1-3. In addition, a test was conducted at the same time as Comparative Example 1 by substituting the American red-breast extract with purified water.

  For each sample, 20 female panelists with dry skin in their 30s to 50s whose skin symptom was remarkably recognized were grouped and used blindly for 1 week, and the skin condition before and after use was observed and evaluated. . As an index of skin symptom, rough skin due to dryness was evaluated in three stages of “improved”, “slightly improved”, and “no change”, and Table 7 shows the number of panelists that obtained each evaluation.

According to Table 7, in the comparative example use group not containing the American red seaweed extract, 60% or more of the panelists were not improved, but in the example use group containing the American red seaweed extract, 60% or more. A clear improvement in rough skin was recognized by the panelists. From this, it was clarified that the extract of the plant belonging to the genus Aberrant has an excellent moisturizing effect.

Claims (4)

A moisturizing agent characterized by comprising an extract of stem and / or root of Oplopanx horridus. A collagen production promoter characterized by comprising an extract of stem and / or root of Oplopanx horridus as an active ingredient. An antioxidant characterized by containing an extract of stem and / or root of Oplopanax horridus as an active ingredient. An agent for promoting the production of vascular endothelial growth factor, comprising an extract of stem and / or root of the genus Opalpanx horridus as an active ingredient.