JP4970442B2 - Whitening agent - Google Patents

Description

  The present invention relates to a moisturizing agent, an anti-aging agent, a whitening agent and an antioxidant containing natural ingredients as active ingredients.

  Causes of skin symptoms such as aging, ultraviolet rays, stress, wrinkles, stains, skin elasticity decrease, dryness, decreased cellular function, melanin production and pigmentation due to ultraviolet rays, decrease or degeneration of dermal matrix components, ultraviolet rays, etc. Examples include oxidative damage. In order to prevent and ameliorate such skin symptoms, search for various active ingredients and formulation studies have been conventionally conducted.

  In particular, naturally-derived components are known to have various pharmacological and cosmetic effects, and so far, applications of extracts such as plants and fungi to skin external preparations have been studied. For example, the essence of Ponkan as an anti-aging agent (see Patent Document 1), a water and / or organic solvent extract of Hakutsuru Reishi as a whitening agent (see Patent Document 2), and an extract of the plant of the genus Sarogase as an antioxidant. (See Patent Document 3) and the like are known.

Moreover, the skin external preparation which mix | blended the betel extract as a plant extract is known (for example, refer patent document 4, 5). However, there are no reports on the investigation of the betel extract as a moisturizer, anti-aging agent, whitening agent, antioxidant, and the moisturizing action, anti-aging action, whitening action and antioxidant action of betel extract.
JP 2001-131045 A JP 2003-89630 A Japanese Patent Laid-Open No. 10-182413 JP-A-6-336417 Japanese Patent Laid-Open No. 10-45540

  There are many naturally derived ingredients whose effects are not yet known, and the development of effective ingredients having excellent moisturizing action, anti-aging action, whitening action and antioxidant action has been expected. The present invention was made in order to find such an active ingredient, and aims to provide a moisturizer, an anti-aging agent, a whitening agent, and an antioxidant that can be applied as an external preparation for skin and food and drink. To do.

  In order to find a moisturizer, an anti-aging agent, a whitening agent, and an antioxidant that can be applied to external preparations for skin and foods and drinks, the present inventors have studied various components derived from nature. As a result, they have found an excellent moisturizing action, anti-aging action, whitening action and antioxidant action in betel extract, and have further studied to complete the present invention. That is, the present invention provides a moisturizer, an anti-aging agent, a whitening agent, and an antioxidant containing a betel extract as an active ingredient. The moisturizer, anti-aging agent, whitening agent and antioxidant used as the active ingredient here are the case where the bean extract is added to the base during the production process, and the bean is used as the base. Including the case of blending.

  According to the present invention, it is possible to provide a moisturizing agent, an anti-aging agent, a whitening agent and an antioxidant having an excellent effect, and by adding these to a composition such as a skin external preparation or food and drink, It is possible to provide various compositions that have an excellent effect in preventing and improving various skin symptoms such as talmi, skin firmness, stains, and kusumi.

  Betel (Scientific name: Piper betle), which is a plant used in the practice of the present invention, is an evergreen vine that belongs to the genus Pepperaceae, native to the Malaysian region.

  When using the betel, it can be used by pulverizing as it is, but it is preferable to use an extract. For extraction, any part such as betel leaves, roots, seeds, and stems may be used, but for convenient use, leaves, stems, and seeds may be used. Or stems. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. The extraction temperature is suitably in the range of about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but can be, for example, about 1 hour to 14 days.

  As extraction solvents, lower alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, ethers such as ethyl ether and propyl ether, butyl acetate and Esters such as ethyl acetate, ketones such as acetone and ethyl methyl ketone, and solvents such as water can be used, and one or more of these are selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  The extract of betel with the above solvent can be used as it is, but it can also be used by concentrating or concentrating to dryness, and dissolving it again in water or a polar solvent. In addition, the extract may be used after subjecting the extract to purification treatment such as decolorization, deodorization, and desalting, and fractionation treatment such as column chromatography. The extract of betel, its treated product and its fraction can be freeze-dried after each treatment and fractionation and dissolved in a solvent before use.

  The extract of betel has excellent moisturizing action, anti-aging action, whitening action and antioxidant action, and can be used as a moisturizing agent, anti-aging agent, whitening agent, antioxidant and external preparation for skin. In addition, moisturizers, anti-aging agents, whitening agents and antioxidants containing betel extract as an active ingredient can be used not only for the skin but also for hair and can be taken orally. Or it can be applied to pharmaceuticals.

  A moisturizing agent containing a betel extract as an active ingredient exhibits an excellent moisturizing effect on the skin and hair, and has a particularly high moisturizing effect on the skin.

  An anti-aging agent comprising a betel extract as an active ingredient exerts an excellent activation effect on various cells, but particularly exhibits an excellent effect on dermal fibroblasts.

  A whitening agent containing an extract of betel as an active ingredient is effective in improving pigmentation symptoms such as spots and freckles, and in particular, is excellent in suppressing melanin production based on inhibition of tyrosinase activity.

  Antioxidants containing betel extract as an active ingredient exhibit an excellent antioxidant effect, but particularly exhibit an excellent effect on free radical scavenging action and superoxide anion scavenging action.

  In addition, by adding the extract of betel to a skin external preparation, a skin external preparation that exhibits an excellent effect in preventing and improving skin symptoms such as wrinkles, talmi, skin firmness, stains, kusumi, dryness, fine lines, etc. It can be obtained, and can also be used as a skin external preparation for improving aging prevention and a skin external preparation for whitening. Furthermore, the extract of betel can also be used for foods and beverages for the purpose of beauty, health maintenance or nutritional supplementation.

  The amount of betel extract to be added to the skin external preparation can be adjusted according to the type of skin external preparation, purpose of use, etc., but from the viewpoint of effect and stability, the total amount is 0.0001. -50.0 mass% is preferable, More preferably, it is 0.001-25.0 mass%.

  The dosage form of the external preparation for skin containing the extract of betel is arbitrary, and can be provided as a solubilizing system such as lotion, an emulsifying system such as cream or milky lotion, or a dispersing system such as calamine lotion. Furthermore, it can also be provided in various dosage forms such as aerosols, ointments, powders and granules filled with a propellant.

  In addition to the betel extract, the topical skin preparation containing betel extract is usually incorporated into pharmaceuticals, quasi drugs, skin cosmetics, hair cosmetics, and cleansing agents as needed. Oily ingredients, moisturizers, powders, pigments, emulsifiers, solubilizers, detergents, UV absorbers, thickeners, drugs, fragrances, resins, antibacterial and antifungal agents, alcohols, etc. Can do. Moreover, in the range which does not impair the effect of this invention, combined use with another moisturizer, an anti-aging agent, a whitening agent, and an antioxidant is also possible.

  Below, the manufacture example of a betel extract and the test for evaluating each effect | action are demonstrated in detail, However, The technical scope of this invention is not limited at all by this.

[Production Example 1]
The betel leaf was dried and pulverized, and a 50 mass% aqueous ethanol solution 20 times the weight of the pulverized product was added, followed by extraction for 2 hours while stirring at room temperature. The extract was collected by filtration, concentrated under reduced pressure, and then freeze-dried to obtain a betel extract of Production Example 1.

[Production Example 2]
Betel leaves were dried and pulverized, purified water of 20 times the weight of the pulverized product was added, and the mixture was extracted by heating at 120 ° C. for 20 minutes in an autograve. In a state where the high temperature was maintained, unnecessary substances were removed by suction filtration, and then lyophilized to obtain a betel extract of Production Example 2.

[Evaluation of action]
1. Evaluation of human dermal fibroblast activation First, human dermal fibroblast activation evaluation of betel extract was performed by the following method. As a sample, the betel extract produced in Production Example 2 was used.

Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. As the seeding medium, Dulbecco's modified Eagle medium (DMEM) to which 1% by mass of fetal bovine serum (FBS) was added was used. After 24 hours, the sample solution was replaced with 1% by mass FBS-added DMEM medium added to each concentration, and further cultured for 48 hours. After removing the supernatant, a medium containing 400 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was added to the cells and cultured for about 2 hours. Finally, formazan produced by the opening of the tetrazolium ring was extracted with 2-propanol, the absorbance at 550 nm and 650 nm was measured with a microplate reader, and the cell activation effect was evaluated using the difference between the two measured values. In the evaluation, in addition to the medium containing the sample solution, 1% by mass FBS-added DMEM medium was used as a negative control, and 5% by mass FBS-added DMEM medium was used as a positive control. The evaluation results are shown in Table 1 as relative values with the cell activation effect in the negative control as 100.

  As is clear from Table 1, in the medium supplemented with betel extract, a significant dermal fibroblast activation effect was observed. In any evaluation in which the betel extract was added in the range of 0.03 to 0.13 mg / mL, a significant dermal fibroblast activation effect was observed in a concentration-dependent manner. From this, it was clarified that the betel extract has an excellent dermal fibroblast activation effect, that is, an anti-aging effect.

2. Evaluation of human dermal fibroblast ATP production ability Next, the evaluation of human dermal fibroblast ATP production ability of betel extract was performed by the following method. As a sample, the betel extract produced in Production Example 1 was used.

Normal human dermal fibroblasts were seeded in a 48-well microplate at 4.0 × 10 ⁴ per well. As the seeding medium, Dulbecco's modified Eagle medium (DMEM) to which 1% by mass of fetal bovine serum (FBS) was added was used. After 24 hours, the sample solution was replaced with 1% by mass FBS-added DMEM medium added to each concentration, and further cultured for 24 hours. The cell supernatant was removed and washed, and the cells were sonicated to elute the ATP in the cells. At that time, in order to prevent elution of ATP-degrading enzyme in the cells, an ATP-degrading enzyme inhibitor (trade name: Cellstein Hoechst 33342) was added to the buffer used for sonication. The prepared cell lysate was dispensed into a test tube, luciferase and a luciferin reagent were added, and chemiluminescence was measured with a compact Lumi VS501 (manufactured by Yamato). The measurement results are shown in Table 2 as relative values with the ATP production ability in the control using the medium without addition of the sample solution as 100.

  As is clear from Table 2, a significant increase in human dermal fibroblast ATP producing ability was observed in the medium containing 0.5 mg / mL betel extract. This revealed that the betel extract has an excellent ability to produce human dermal fibroblast ATP, that is, has an anti-aging effect.

3. Evaluation of antioxidant ability using DPPH Next, the antioxidant activity of betel extract was evaluated by the following method. As a sample, the betel extract produced in Production Example 1 was used.

  100 μL of a sample solution diluted to each concentration with a 50 mass% aqueous ethanol solution was added to a 96-well microplate. To this, 100 μL of 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared in ethanol to a concentration of 0.2 mM was added and mixed well, and then at room temperature in the dark. Left for 10 minutes. Thereafter, the absorbance at 516 nm derived from the DPPH radical was measured. When the absorbance when the sample solution was not added was used as (A1) and the absorbance when the sample was added as (B1), the value calculated by equation (1) was taken as the radical elimination rate. The evaluation results are shown in Table 3.

  Formula (1) {1- (B1) / (A1)} × 100 (%)

  As is apparent from Table 3, the betel extract was found to have an antioxidant action based on radical scavenging.

4). SOD-like activity evaluation (superoxide anion scavenging ability evaluation)
Next, the SOD-like activity of the betel extract was evaluated by the following method. As a sample, the betel extract produced in Production Example 2 was used.

  To 75 μL of the HANK ′S (+) solution containing 0.25 mM WST-1 and 1 mM Hypoxanthine, 25 μL of the sample solution prepared to an arbitrary concentration with the HANK ′S (+) solution was added. Further, 25 μL (0.0075 Units) of Xanthine Oxidase was added, and after reacting at 37 ° C. for 15 minutes, the absorbance at 450 nm was measured. When the absorbance when only HANK'S (+) is added instead of the sample solution is (A2), and the absorbance when the sample solution is added is (B2), the value calculated by equation (2) is the superoxide anion. The elimination rate was used. The evaluation results are shown in Table 4.

  Formula (2) {1- (B2) / (A2)} × 100 (%)

  As is clear from Table 4, it was found that the betel extract has an antioxidant action based on elimination of superoxide anion in a concentration-dependent manner.

5. Evaluation of inhibition of human epidermal melanocyte tyrosinase activity Next, evaluation of inhibition of human epidermal melanocyte tyrosinase activity of betel extract was carried out by the following method. As a sample, the betel extract produced in Production Example 2 was used.

Normal human epidermal melanocytes were seeded in a 96-well microplate at 3.0 × 10 ⁴ cells per well. Medium 154S was used as the seeding medium. After 24 hours, the sample solution was replaced with Medium 154S added to each concentration, and further cultured for 48 hours. Next, the solution was exchanged with 75 μL of a phosphate buffer containing 1% by mass of Triton-X to completely lyse the cells, and 50 μL was used as a crude enzyme solution. 50 μL of 0.05% by mass L-dopa-containing phosphate buffer as a substrate was added to the crude enzyme solution, and the mixture was allowed to stand at 37 ° C. for 2 hours. The absorbance at 405 nm immediately after addition of the substrate and the absorbance at 405 nm at the end of the reaction were measured with a microplate reader, and the value calculated by equation (3) was taken as the amount of dopamelanin. In the formula (3), the absorbance at 405 nm immediately after addition of the substrate is (A3), and the absorbance at 405 nm at the end of the reaction is (B3).

Formula (3) {(B3)-(A3) -2.166} /2.238
Then, the amount of protein in each well was measured using BCA Protein Assay Kit manufactured by PIERCE, and the amount of dopamelanin per 1 μg of protein was determined. The amount of dopamelanin in the case of using a medium with no sample solution added was used as a negative control. The evaluation results are shown in Table 5.

  As is clear from Table 5, it was found that the betel extract has an activity to inhibit tyrosinase activity. That is, it was found that the betel extract has a whitening effect.

6). Evaluation of ability to inhibit melanin production using B16 mouse melanoma cells Next, the ability of betel extract to inhibit melanin production was evaluated by the following method. As a sample, the betel extract produced in Production Example 2 was used.

  Mouse melanoma cells (B16F0 strain) were seeded in a 90 mm dish so that there were 18000 cells per dish. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS). After 24 hours, the sample solution was replaced with a 5% by mass FBS-added DMEM medium added to each concentration, and further cultured for 5 days. After completion of the culture, cells were peeled off by trypsin treatment, transferred to a 1.5 mL microtube, and centrifuged to obtain a cell precipitate. The resulting precipitate was visually evaluated for its blackening status based on the following criteria. In the evaluation, a 5% FBS-added DMEM medium containing 5% by mass FBS was used as a negative control, and a 5% FBS-added DMEM medium containing 50 mM sodium lactate was used as a positive control. These visual determination results were “5” and “1”, respectively, and used as an index for sample determination. The visual judgment was evaluated in five stages as shown below.

1: Same as positive control (almost white)
2: Slightly blacker than the positive control (light brown)
3: Between positive control and negative control (brown)
4: Slight blackening is suppressed compared to the negative control (blackish brown)
5: Same as negative control (almost black)
At the same time, a tissue solubilizer (trade name: Solvable) was added to the precipitate, boiled, returned to room temperature, and the absorbance at 500 nm was measured with a spectrophotometer (spectrophotometer U-3010, manufactured by HITACHI). The evaluation results are shown in Table 6.

  As is clear from the visual evaluation and total melanin amount measurement in Table 6, it was found that the betel extract has a melanin production-inhibiting ability, that is, has a whitening effect.

  Then, the formulation example of the skin external preparation which mix | blended the betel extract which concerns on this invention is shown. In the following formulation examples, the amount of each component is expressed in mass%.

[Formulation Example 1] Emulsion (1) Squalane 10.0 (mass%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polystearic acid polyoxyethylene sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 53.85
(11) Arginine (1% by weight aqueous solution) 20.0
(12) Betel extract [Production Example 1] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix uniformly.

[Prescription Example 2] Lotion (1) Ethanol 15.0 (mass%)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 78.38
(5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Betel extract [Production Example 2] 5.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.

[Prescription Example 3] Cream (1) Squalane 10.0 (mass%)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20 mass% aqueous solution) 15.0
(10) Purified water 40.7
(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Betel extract [Production Example 1] 1.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification is completed, add (11), start cooling, add (12) at 40 ° C., and mix uniformly.

[Formulation Example 4] Cosmetic liquid (1) Purified water 27.45 (mass%)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1% by weight aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by mass aqueous solution) 2.0
(16) Betel extract [Production Example 2] 5.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C, add (16) and mix evenly.

[Formulation Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (mass%)
(2) Purified water 86.7
(3) Sodium hydroxide (10% by mass aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Betel extract [Production Example 1] 2.0
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Formulation Example 6] Cleansing Fee (1) Squalane 81.0 (mass%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Purified water 3.0
(4) Betel extract [Production Example 2] 1.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.

[Formulation Example 7] Face-wash foam (1) Stearic acid 16.0 (mass%)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 36.5
(8) Betel extract [Production Example 1] 1.0
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.

[Prescription Example 8] Make-up base cream (1) Squalane 10.0 (mass%)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 69.4
(8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Betel extract [Production Example 2] 1.2
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Prescription Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (mass%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Purified water 57.4
(11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Betel extract [Production Example 1] 1.0
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Formulation Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by mass)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Betel extract [Production Example 1] 1.0
(11) Purified water 47.4
(12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. while stirring and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.

[Prescription Example 11] Pack (1) Purified water 58.9 (mass%)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 5.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Betel extract [Production Example 1] 5.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.

[Prescription Example 12] Bath agent (1) Fragrance 0.3 (% by mass)
(2) Betel extract [Production Example 2] 1.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 48.7
Production method: (1) to (4) are mixed uniformly.

[Prescription Example 13] Hair wax (1) Stearic acid 3.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 73.6
(11) Betel extract [Production Example 1] 2.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Prescription Example 14] Hairtonic (1) Ethanol 50.0 (mass%)
(2) Purified water 48.9
(3) Betel extract [Production Example 2] 1.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

[Prescription Example 15] Beverage (1) Betel extract [Production Example 2] 8.0 (mass%)
(2) Erythritol 1.0
(3) Citric acid 0.1
(4) Stevia 0.01
(5) Purified water 90.89
Production method: (1) to (5) are mixed uniformly.

  Next, a use test was conducted using a prescription formulated with betel extract, and the improvement effect was evaluated for rough skin caused by drying. At that time, the emulsion of Formulation Example 1 as Example 1 and the emulsion using the betel extract of Production Example 2 instead of the betel extract of Production Example 1 of Example 1 as Example 2 were carried out as Comparative Example 1. A milky lotion using a 50% by mass ethanol aqueous solution instead of the betel extract of Example 1 was prepared as a comparative example 2, and an emulsion using purified water instead of the betel extract of Example 1 was prepared as a sample.

  For each sample, 20 female panelists with dry skin in their 30s to 50s whose skin symptom was remarkably recognized were grouped and used blindly for 1 week, and the skin condition before and after use was observed and evaluated. . As an index of skin symptom, rough skin due to dryness was evaluated in three stages of “improved”, “slightly improved”, and “no change”, and Table 7 shows the number of panelists that obtained each evaluation.

  From Table 7, in the comparative example use group containing no betel extract, 80% or more of the panelers were not improved, but in the example use group containing the betel extract, 75% or more of the panelers were not improved. A clear improvement in rough skin was observed. This revealed that betel extract has an excellent moisturizing effect.

  From the foregoing description, many modifications and other embodiments of the present invention are obvious to one skilled in the art. Accordingly, the foregoing description should be construed as illustrative only and is provided for the purpose of teaching those skilled in the art the best mode of carrying out the invention. The details of the embodiments can be varied substantially without departing from the spirit of the invention.

  The moisturizing agent, anti-aging agent, whitening agent and antioxidant of the present invention are used by blending them with external preparations for skin and foods such as pharmaceuticals, quasi-drugs, skin cosmetics, cosmetics for hair or cleaning products. Useful for.

Claims (1)

A whitening agent containing a hot water extract of betel leaves as an active ingredient.