JP4916150B2 - Moisturizer, cell activator, antioxidant, and skin external preparation, - Google Patents

Description

  The present invention relates to a humectant, a cell activator, an antioxidant, and a skin external preparation. More specifically, the present invention relates to a moisturizer, a cell activator, an antioxidant, an external preparation for skin, and a food containing an extract of Fusazakura family plants.

  Causes of skin symptoms such as aging, ultraviolet rays, stress, wrinkles, stains, skin elasticity decrease, dryness, decreased cellular function, melanin production and pigmentation due to ultraviolet rays, decrease or degeneration of dermal matrix components, ultraviolet rays, etc. Examples include oxidative damage. In order to prevent and ameliorate such skin symptoms, search for various active ingredients and formulation studies have been conventionally conducted. As the cell activator, the essence of Ponkan (see Patent Document 1) is known, and as the antioxidant, an extract of the plant belonging to the genus Sarogase (see Patent Document 2) is known.

  In addition, the prior art regarding the moisturizer, cell activator, antioxidant, and skin external preparation which uses the extract of Fusazakura family plant as an active ingredient was not recognized.

JP 2001-131045 A JP 2003-89630 A

  Naturally-derived components are known to have various pharmacological and cosmetic effects, and so far many plants and fungi have been widely applied to fields such as external preparations for skin and foods and drinks. However, there are many naturally-derived components whose effects are not yet known, and the development of active ingredients having excellent moisturizing action, cell activation action, antioxidant action and the like has been expected. The present invention has been made in order to find such an active ingredient, and an object thereof is to provide a moisturizer, a cell activator, and an antioxidant that can be widely applied in the field of external preparations for skin and foods and drinks. And

  In order to find a moisturizer, a cell activator, a whitening agent, and an antioxidant that can be widely applied to fields such as external preparations for skin and foods and drinks, the present inventors have studied various substances derived from nature. It was. As a result, the present inventors have found an excellent moisturizing action, cell activation action, and antioxidant action for the extract of Fusazakura family plants, and have further studied to complete the present invention. That is, the present invention provides a skin external preparation containing a moisturizer, a cell activator, a whitening agent, an antioxidant, and an extract of one or more plants of the Fusaza cherry plant, which contains Fusaza cherry plants as active ingredients. An agent and food are provided.

  According to the present invention, it is possible to provide a moisturizer, a cell activator, a whitening agent, and an antioxidant having an excellent effect, and by blending them in a composition such as a skin external preparation or food, It is possible to provide various compositions that exhibit an excellent effect in preventing and improving various skin symptoms such as talmi, skin firmness, spots, and kusumi.

Euptelea plant is a plant that is used as a raw material of the present invention (Eupteleaceae) is, Japan is a unique species Fusazakura (Euptelea polyandra), Shinafusazakura distributed over the Himalayas eastern from western China (Euptelea pleiosperma) is known.

  When using Fusazakura family plants, they can be crushed as they are, but it is preferable to use an extract. For extraction, any part of the stem, branches, leaves, flowers, seeds, bark, sap, roots, stems, buds, etc. of Fussakura family may be used. Seeds are preferably used, and leaves and stems are preferably used from the viewpoint of effectiveness. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days.

  Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, ethers such as ethyl ether and propyl ether. And solvents such as esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  The extract of the above-mentioned Fusazakura plant with the above solvent can be used as it is, but the concentrated and dried product can be used again by dissolving it in water or a polar solvent, and the physiological effects thereof are not impaired. It may be used after performing purification treatment such as decolorization, deodorization and desalting, and fractionation treatment by column chromatography. The said extract of a Fusazakura family plant, its processed material, and a fraction are freeze-dried after each process and fractionation, It can also be melt | dissolved and used for a solvent at the time of use.

  The extract of Fusazakura plant has excellent moisturizing action, cell activation action, and antioxidant action, and can be used as a moisturizer, cell activator, antioxidant, and skin external preparation. In addition, moisturizers, cell activators, and antioxidants, which are extracts of Fusazakura family plants, can be used not only for the skin but also for hair and can be taken orally. Alternatively, it can be applied to pharmaceuticals.

  A moisturizing agent containing an extract of the Fusazakura family plant as an active ingredient exhibits an excellent moisturizing effect on the skin and hair, and has a particularly high moisturizing effect on the skin.

  A cell activator comprising an extract of Fusazakura plant as an active ingredient exerts an excellent activation action on various cells, but particularly exhibits an excellent effect on dermal fibroblasts.

  Antioxidants containing an extract of Fusazakura plant as an active ingredient exhibit an excellent antioxidant effect, but particularly exhibit an excellent effect on scavenging free radicals.

  In addition, by incorporating the extract of Fusazakura plant into an external preparation for skin, it is effective for the prevention and improvement of skin symptoms such as wrinkles, talmi, skin firmness, spots, kumi, dryness, fine lines, etc. Can be obtained, and can also be used as a skin external preparation for moisturizing, a skin external preparation for improving anti-aging, or a skin external preparation for whitening. Furthermore, the extract of the Fusazakura family plant can also be used for foods and beverages for the purpose of beauty, health maintenance or nutritional supplementation.

  The amount of Fusazakura family plant extract blended into the skin external preparation can be adjusted according to the type of skin external preparation, purpose of use, etc., but it is 0 with respect to the total amount in terms of effects and stability. 0.0001 to 50.0% by weight is preferable, and 0.001 to 25.0% by weight is more preferable.

  The dosage form of the external preparation for skin containing the extract of Fusazakura family plant is arbitrary, and for example, it can be provided as a solubilizing system such as lotion, an emulsifying system such as cream or emulsion, or a dispersing system such as calamine lotion. Furthermore, it can also be provided in various dosage forms such as aerosols, ointments, powders and granules filled with a propellant.

  In addition, for the topical skin preparation containing the extract of Fusazakura plant, in addition to the extract of Fusazakura plant, if necessary, it is usually a pharmaceutical, quasi-drug, skin cosmetic, hair cosmetic and washing. Oil components, moisturizers, powders, pigments, emulsifiers, solubilizers, cleaning agents, UV absorbers, thickeners, drugs, fragrances, resins, antibacterial and antifungal agents, alcohols, etc. It can mix | blend suitably. Moreover, in the range which does not impair the effect of this invention, combined use with another moisturizer, a cell activator, or an antioxidant is also possible.

  In the following, the production example of the extract of Fusazakura family plant, the test for evaluating each action, the formulation example as a skin external preparation and food, and the use test will be described in more detail, but the technical scope of the present invention is based on this. It is not limited at all.

[Production Example 1]
10 kg of a 50 wt% aqueous ethanol solution was added to 1 kg of a dry pulverized whole plant of Fusazakura family plants, and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, an extract of Fusazakura plant was obtained.

[Production Example 2]
Nine liters of water was added to 1 kg of a dry pulverized whole plant of Fusazakura, and the mixture was extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration, and after removing the solvent, an extract of Fusazakura plant was obtained.

[Production Example 3]
Nine liters of methanol was added to 1 kg of dried ground pulverized plants of Fusazakura family plants, and they were immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, an extract of Fusazakura plant was obtained.

[Production Example 4]
The whole plant of the Frostaceae plant was put into a supercritical extraction apparatus, and extraction was performed using a supercritical fluid of carbon dioxide at 40 ° C. under a pressure of 15 MPa. The extract was collected to obtain an extract of Fusazakura family plants.

  First, it shows about the cell activation effect | action of the extract of a Fusazakura family plant. As the sample, the sample obtained by extracting the whole plant of Sinafu cherry according to Production Example 1 was evaluated.

The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum. After culturing for 24 hours, the culture medium was replaced with a test medium to which a sample having an arbitrary concentration was added, and further cultured for 48 hours. Next, the medium containing 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was replaced with a medium containing 400 μg / mL and cultured for 2 hours. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in Table 1 as relative values with the cell activation effect in the blank with no sample as 100. Note that * and ** in the table represent a significance probability of less than 1% (P <0.01) by ** with respect to the significance probability P value in the t test.

  As is clear from Table 1, a significant dermal fibroblast activation effect was observed in the medium supplemented with the extract of Fusazakura family plants. From this, it was clarified that the extract of Fusazakura family has an excellent cell activation effect.

  Next, it shows about the antioxidant effect of the extract of Fusazakura plant. As the sample, the sample obtained by extracting the whole plant of Sinafu cherry according to Production Example 1 was evaluated as Sample 2.

The evaluation was performed according to the following procedure. 100 μL of an extract solution of Fusasa cherry family plant diluted to an arbitrary concentration with a 50 wt% aqueous ethanol solution was added to a 96-well microplate. Next, 100 μL of a 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared in ethanol to a concentration of 0.2 mM was added to a 96-well microplate. The mixture was left in the dark with stirring, and the absorbance at 516 nm was measured after 24 hours. When the absorbance of the blank with no sample added was (A) and the absorbance when the sample was added was (B), the value of formula (1) was defined as the radical elimination rate. The evaluation results are shown in Table 4.
Formula (1) {1- (B) / (A)} × 100 (%)

  As is clear from Table 2, it was found that the extract of Fusazakura family plant has an antioxidant action based on radical scavenging.

  Then, the example of a prescription of the skin external preparation which mix | blended the extract of the Fusazakura family plant concerning this invention is shown.

[Formulation Example 1] Emulsion (1) Squalane 10.0 (wt%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 53.85
(11) Arginine (1 wt% aqueous solution) 20.0
(12) Fusazakura family plant extract [Production Example 1] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix uniformly.

[Prescription Example 2] Lotion (1) Ethanol 15.0 (% by weight)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 78.38
(5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Fusazakura plant extract [Production Example 3] 5.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.

[Prescription Example 3] Cream (1) Squalane 10.0 (% by weight)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20% by weight aqueous solution) 15.0
(10) Purified water 40.7
(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Fusazakura family plant extract [Production Example 1] 1.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification is completed, add (11), start cooling, add (12) at 40 ° C., and mix uniformly.

[Formulation Example 4] Cosmetic liquid (1) Purified water 27.45 (% by weight)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1 wt% aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by weight aqueous solution) 2.0
(16) Fusazakura family plant extract [Production Example 1] 5.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C, add (16) and mix evenly.

[Formulation Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (% by weight)
(2) Purified water 86.7
(3) Sodium hydroxide (10% by weight aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Fusazakura family plant extract [Production Example 4] 2.0
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Formulation Example 6] Cleansing Fee (1) Squalane 81.0 (wt%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Purified water 3.0
(4) Fusazakura plant extract [Production Example 4] 1.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.

[Prescription Example 7] Face-wash foam (1) Stearic acid 16.0 (% by weight)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 36.5
(8) Fusazakura plant extract [Production Example 3] 1.0
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.

[Prescription Example 8] Make-up base cream (1) Squalane 10.0 (% by weight)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 69.4
(8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Extract of Fusazacra family [Production Example 2] 1.2
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Formulation Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (wt%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Purified water 57.4
(11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Fusazakura plant extract [Production Example 4] 1.0
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Formulation Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Fusazakura family plant extract [Production Example 1] 1.0
(11) Purified water 47.4
(12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. while stirring and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.

[Prescription Example 11] Pack (1) Purified water 58.9 (% by weight)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 5.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Fusazakura family plant extract [Production Example 2] 5.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.

[Prescription Example 12] Bath agent (1) Fragrance 0.3 (% by weight)
(2) Fusazakura family plant extract [Production Example 1] 1.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 48.7
Production method: (1) to (4) are mixed uniformly.

[Prescription Example 13] Hair wax (1) Stearic acid 3.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 73.6
(11) Fusazakura plant extract [Production Example 1] 2.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Prescription Example 14] Hair artic (1) Ethanol 50.0 (% by weight)
(2) Purified water 48.9
(3) Fusazakura family plant extract [Production Example 3] 1.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

[Prescription Example 15] Beverage (1) Extract from Fusazakura Family [Production Example 1] 8.0 (wt%)
(2) Erythritol 1.0
(3) Citric acid 0.1
(4) Stevia 0.01
(5) Purified water 90.89
Production method: (1) to (5) are mixed uniformly.

  Next, a use test was conducted using a prescription blended with an extract of Fusazakura family plants, and the effect of improving the rough skin due to drying was evaluated. At that time, the extract of Fusazakura plant listed in Table 3 was blended with the emulsion formulation shown in Formulation Example 1, and the usage test was conducted as Examples 1-3. Moreover, the extract of the Fusazakura family was replaced with purified water, and a use test was simultaneously conducted as Comparative Example 1.

  For each sample, 20 female panelists with dry skin in their 30s to 50s whose skin symptom was remarkably recognized were grouped and used blindly for 1 week, and the skin condition before and after use was observed and evaluated. . As an index of skin symptoms, rough skin due to dryness was evaluated in three stages of “improvement”, “slight improvement”, and “no change”, and Table 4 shows the number of panelists that obtained each evaluation.

From Table 4, in the comparative example use group that does not contain the extract of Fusazakura family plant, improvement was not recognized in more than 60% of the panelists, but in the Example use group that blended the extract of Fusazakura family plant, More than 60% of panelists showed a clear improvement in rough skin. From this, it was clarified that the extract of Fusazakura plant has an excellent moisturizing effect.

Claims (3)

A moisturizing agent comprising, as an active ingredient, an extract of one or more kinds of plants selected from Fusazakura family plants. A cell activator comprising, as an active ingredient, an extract of one or two or more kinds of plants selected from Fusazakura family plants. The antioxidant which uses the extract of the 1 type, or 2 or more types of plant chosen from the Fusazakura family plant as an active ingredient.