JP4488705B2 - Cell activator, hyaluronic acid production promoter, decorin production promoter, antioxidant, and skin external preparation - Google Patents
Cell activator, hyaluronic acid production promoter, decorin production promoter, antioxidant, and skin external preparation Download PDFInfo
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- JP4488705B2 JP4488705B2 JP2003295752A JP2003295752A JP4488705B2 JP 4488705 B2 JP4488705 B2 JP 4488705B2 JP 2003295752 A JP2003295752 A JP 2003295752A JP 2003295752 A JP2003295752 A JP 2003295752A JP 4488705 B2 JP4488705 B2 JP 4488705B2
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Description
本発明は、細胞賦活剤、ヒアルロン酸産生促進剤、デコリン産生促進剤、抗酸化剤、及び皮膚外用剤に関する。さらに詳しくは、シッポゴケ科（Ｄｉｃｒａｎａｃｅａｅ）植物より選ばれる１種又は２種以上の植物の抽出物を含有する細胞賦活剤、ヒアルロン酸産生促進剤、デコリン産生促進剤、抗酸化剤、及び皮膚外用剤に関する。 The present invention relates to a cell activator, hyaluronic acid production promoter, decorin production promoter, antioxidant, and skin external preparation. More specifically, a cell activator, a hyaluronic acid production promoter, a decorin production promoter, an antioxidant, and a skin external preparation containing an extract of one or two or more plants selected from the plant of Dicranaceae About.
加齢や紫外線などによるシワ，シミ，皮膚の弾性低下といった老化症状の要因として、細胞機能低下、ヒアルロン酸やデコリン等の真皮マトリックス成分の減少や変性、紫外線等による細胞の酸化傷害などが挙げられる。このような老化症状を防止・改善するために、様々な有効成分の検索及び配合検討が従来なされてきた。細胞賦活剤としては、ポンカンのエッセンス（特許文献１参照）、ヒアルロン酸産生促進剤としては、アナアオサの抽出物（特許文献２参照）、デコリン産生促進剤としては、ペトロセリン酸（特許文献３参照）、抗酸化剤としては、サルオガセ科サルオガセ属植物の抽出物（特許文献４参照）が知られている。 Factors of aging symptoms such as wrinkles, blemishes, and decreased skin elasticity due to aging and ultraviolet rays include decreased cellular function, decreased or degenerated dermal matrix components such as hyaluronic acid and decorin, and oxidative damage of cells due to ultraviolet rays, etc. . In order to prevent and ameliorate such aging symptoms, search for various active ingredients and formulation studies have been conventionally conducted. As a cell activator, the essence of Ponkan (see Patent Document 1), as a hyaluronic acid production promoter, an extract of Anaanaosa (see Patent Document 2), and as a decorin production promoter, Petroceric acid (see Patent Document 3) As an antioxidant, an extract of a plant belonging to the genus Salogaceae (see Patent Document 4) is known.
なお、シッポゴケ科植物の抽出物を有効成分とする細胞賦活剤、ヒアルロン酸産生促進剤、デコリン産生促進剤、抗酸化剤、及び皮膚外用剤に関する先行技術は認められなかった。 In addition, the prior art regarding the cell activator, hyaluronic acid production promoter, decorin production promoter, antioxidant, and skin external preparation which use the extract of a plant of the genus Schipogokeaceae as an active ingredient was not recognized.
従来用いられている細胞賦活剤、ヒアルロン酸産生促進剤、デコリン産生促進剤、抗酸化剤は、本質的な効果としては不十分な場合もあり、より優れた有効成分の開発が期待されていた。本発明は、このような従来の問題点に鑑みてなされたものであり、天然由来で安全性が高く、優れた細胞賦活作用、ヒアルロン酸産生促進作用、デコリン産生促進作用、抗酸化作用を有する有効成分を見出し、老化防止・改善に有用な皮膚外用剤を提供することを目的とする。 Conventionally used cell activators, hyaluronic acid production promoters, decorin production promoters and antioxidants may be insufficient as essential effects, and the development of better active ingredients was expected. . The present invention has been made in view of such conventional problems, and is naturally derived and highly safe, and has an excellent cell activation effect, hyaluronic acid production promoting effect, decorin production promoting effect, and antioxidant effect. The object is to find an active ingredient and to provide a skin external preparation useful for the prevention and improvement of aging.
本発明者らは、上記の課題を解決するために、老化防止・改善に有用な作用に関して、天然由来の種々の物質について検討を行った。その結果、シッポゴケ科植物より選ばれる１種又は２種以上の植物の抽出物に優れた細胞賦活作用、ヒアルロン酸産生促進作用、デコリン産生促進作用、抗酸化作用を見出し、さらに検討を重ね、本発明を完成するに至った。すなわち、本発明は、シッポゴケ科植物の１種または２種以上の植物の抽出物を有効成分とする細胞賦活剤、ヒアルロン酸産生促進剤、デコリン産生促進剤、抗酸化剤、及び皮膚外用剤を提供するものである。 In order to solve the above-described problems, the present inventors have studied various naturally-derived substances with respect to actions useful for preventing and improving aging. As a result, we have found excellent cell activation, hyaluronic acid production promotion, decorin production promotion, and antioxidant activity in extracts of one or more plants selected from the plant of the genus Sphagnumaceae. The invention has been completed. That is, the present invention provides a cell activator, a hyaluronic acid production promoter, a decorin production promoter, an antioxidant, and an external preparation for skin, each comprising an extract of one or more plant species of the plant belonging to the family Chypsumaceae. It is to provide.
本発明によれば、優れた効果を有する細胞賦活剤、ヒアルロン酸産生促進剤、デコリン産生促進剤、抗酸化剤を提供することができる。また、これらを皮膚外用剤に配合することにより、シワ，タルミ，肌のハリ，シミ，クスミといった皮膚老化症状の防止・改善に優れた効果を発揮する老化防止改善用の皮膚外用剤や食品等の組成物を提供することができる。 According to the present invention, it is possible to provide a cell activator, hyaluronic acid production promoter, decorin production promoter, and antioxidant that have excellent effects. In addition, by adding these to skin external preparations, skin external preparations and foods for improving aging prevention that exhibit excellent effects in the prevention and improvement of skin aging symptoms such as wrinkles, tarmi, skin firmness, spots, and scum The composition can be provided.
本発明の原料として用いられる植物は、シッポゴケ科（Ｄｉｃｒａｎａｃｅａｅ）の植物であればよい。シッポゴケ科植物としては、ハタキゴケ属（Ａｏｎｇｓｔｒｏｅｍｉａ），キシッポゴケ属（Ａｒｃｔｏａ），シシゴケ属（Ｂｒｏｔｈｅｒａ），ブルッフゴケ属（Ｂｒｕｃｈｉａ），ツリバリゴケモドキ属（Ｂｒｙｏｈｕｍｂｅｒｔｉａ），ヘビゴケ属（Ｃａｍｐｙｌｏｐｏｄｉｕｍ），ツリバリゴケ属（Ｃａｍｐｙｌｏｐｕｓ），イヌノハゴケ属（Ｃｙｎｏｄｏｎｔｉｕｍ），クマデゴケ属（Ｄｉｃｈｏｄｏｎｔｉｕｍ），ススキゴケ属（Ｄｉｃｒａｎｅｌｌａ），ユミゴケ属（Ｄｉｃｒａｎｏｄｏｎｔｉｕｍ），ヘリトリシッポゴケ属（Ｄｉｃｒａｎｏｌｏｍａ），オウギゴケ属（Ｄｉｃｒａｎｏｗｅｉｓｉａ），シッポゴケ属（Ｄｉｃｒａｎｕｍ），ニセタマウケゴケ属（Ｇａｒｃｋｅａ），マイマイゴケ属（Ｈｏｌｏｍｉｔｒｉｕｍ），カマシッポゴケ属（Ｋｉａｅｒｉａ），マツバゴケ属（Ｌｅｕｃｏｌｏｍａ），コブゴケ属（Ｏｎｃｈｏｐｈｏｒｕｓ），ヤマゴケ属（Ｏｒｅａｓ），ミヤマゴケ属（Ｏｒｅｏｗｅｉｓｉａ），ナガバノシッポゴケ属（Ｐａｒａｌｅｕｃｏｂｒｙｕｍ），ヤスジゴケ属（Ｒｈａｂｄｏｗｅｉｓｉａ），ナガダイゴケ属（Ｔｒｅｍａｔｏｄｏｎ）など５０属余りが知られている。ハタキゴケ属の植物としては、フジサンギンゴケモドキ（Ａｏｎｇｓｔｒｏｅｍｉａ ｊｕｌａｃｅａ）などが知られている。キシッポゴケ属の植物としては、キシッポゴケ（Ａｒｃｔｏａ ｆｕｌｖｅｌｌａ），ナガエキシッポゴケ（Ａｒｃｔｏａ ｆｕｌｖｅｌｌａ ｖａｒ． ｌｏｎｇｉｓｅｔａｃｅａ），タカネカモジゴケ（Ａｒｃｔｏａ ｓｃｈｉｓｔｉｏｉｄｅｓ）などが知られている。シシゴケ属の植物としては、シシゴケ（Ｂｒｏｔｈｅｒａ ｌｅａｎａ）などが知られている。ブルッフゴケ属の植物としては、ブルッフゴケ（Ｂｒｕｃｈｉａ ｍｉｃｒｏｓｐｏｒａ）などが知られている。ツリバリゴケモドキ属の植物としては、ツリバリゴケモドキ（Ｂｒｙｏｈｕｍｂｅｒｔｉａ ｓｕｂｃｏｍｏｓａ）などが知られている。ヘビゴケ属の植物としては、ヘビゴケ（Ｃａｍｐｙｌｏｐｏｄｉｕｍ ｍｅｄｉｕｍ）などが知られている。ツリバリゴケ属の植物としては、クロツリバリゴケ（Ｃａｍｐｙｌｏｐｕｓ ａｔｒｏｖｉｒｅｎｓ），マユハケゴケ（Ｃａｍｐｙｌｏｐｕｓ ｆｒａｇｉｌｉｓ），ヤマトフデゴケ（Ｃａｍｐｙｌｏｐｕｓ ｊａｐｏｎｉｃｕｓ），ヒロスジツリバリゴケ（Ｃａｍｐｙｌｏｐｕｓ ｓｃｈｗａｒｚｉｉ），フデゴケ（Ｃａｍｐｙｌｏｐｕｓ ｕｍｂｅｌｌａｔｕｓ），ヤクシマツリバリゴケ（Ｃａｍｐｙｌｏｐｕｓ ｙａｋｕｓｈｉｍｅｎｓｉｓ）などが知られている。イヌノハゴケ属の植物としては、オカイヌノハゴケ（Ｃｙｎｏｄｏｎｔｉｕｍ ｆａｌｌａｘ），ミヤマイヌノハゴケ（Ｃｙｎｏｄｏｎｔｉｕｍ ｇｒａｃｉｌｅｓｃｅｎｓ），イヌノハゴケ（Ｃｙｎｏｄｏｎｔｉｕｍ ｐｏｌｙｃａｒｐｕｍ），コブゴケ（Ｃｙｎｏｄｏｎｔｉｕｍ ｓｔｒｕｍｉｆｅｒｕｍ），ヒメイヌノハゴケ（Ｃｙｎｏｄｏｎｔｉｕｍ ｔｅｎｅｌｌｕｍ）などが知られている。クマデゴケ属の植物としては、シメリイワゴケ（Ｄｉｃｈｏｄｏｎｔｉｕｍ ｐｅｌｌｕｃｉｄｕｍ），クマデゴケ（Ｄｉｃｈｏｄｏｎｔｉｕｍ ｐｅｌｌｕｃｉｄｕｍ ｖａｒ． ｊａｐｏｎｉｃｕｍ），エゾクマデゴケ（Ｄｉｃｈｏｄｏｎｔｉｕｍ ｐｅｌｌｕｃｉｄｕｍ ｖａｒ． ｙｅｚｏｅｎｓｅ），イボイワゴケ（Ｄｉｃｈｏｄｏｎｔｉｕｍ ｖｅｒｒｕｃｏｓｕｍ）などが知られている。ススキゴケ属の植物としては、アオモリススキゴケ（Ｄｉｃｒａｎｅｌｌａ ｂｒａｃｈｙａｎｇｉａ），コブオバナゴケ（Ｄｉｃｒａｎｅｌｌａ ｃｅｒｖｉｃｕｌａｔａ），ホウライオバナゴケ（Ｄｉｃｒａｎｅｌｌａ ｃｏａｒｃｔａｔａ），ミチノクオバナゴケ（Ｄｉｃｒａｎｅｌｌａ ｄｉｌａｔａｔｉｎｅｒｖｉｓ），キンシゴケモドキ（Ｄｉｃｒａｎｅｌｌａ ｄｉｔｒｉｃｈｏｉｄｅｓ），タマススキゴケ（Ｄｉｃｒａｎｅｌｌａ ｇｌｏｂｕｌｉｇｅｒａ），イヨススキゴケ（Ｄｉｃｒａｎｅｌｌａ ｇｏｎｏｉ），ススキゴケ（Ｄｉｃｒａｎｅｌｌａ ｈｅｔｅｒｏｍａｌｌａ），ユガミススキゴケ（Ｄｉｃｒａｎｅｌｌａ ｈｅｔｅｒｏｍａｌｌａ ｖａｒ． ｃｕｒｖｉｐｅｓ），コススキゴケ（Ｄｉｃｒａｎｅｌｌａ ｉｉｓｉｂａｅ），シマススキゴケ（Ｄｉｃｒａｎｅｌｌａ ｊａｖａｎｉｃａ），ツクシススキゴケ（Ｄｉｃｒａｎｅｌｌａ ｋｉｕｓｈｉａｎａ），ツクシハナガゴケ（Ｄｉｃｒａｎｅｌｌａ ｍａｙｅｂａｒａｅ），オオシマススキゴケ（Ｄｉｃｒａｎｅｌｌａ ｏｓｈｉｍａｅ），ヒロハススキゴケ（Ｄｉｃｒａｎｅｌｌａ ｐａｌｕｓｔｒｉｓ），エゾノオバナゴケ（Ｄｉｃｒａｎｅｌｌａ ｓｃｈｒｅｂｅｒｉａｎａ），ミヤマススキゴケ（Ｄｉｃｒａｎｅｌｌａ ｓｕｂｓｅｃｕｎｄａ），カラフトススキゴケ（Ｄｉｃｒａｎｅｌｌａ ｓｕｂｕｌａｔａ），ナガスジススキゴケ（Ｄｉｃｒａｎｅｌｌａ ｖａｒｉａ），エゾススキゴケ（Ｄｉｃｒａｎｅｌｌａ ｙｅｚｏａｎａ）などが知られている。ユミゴケ属の植物としては、カタバユミゴケ（Ｄｉｃｒａｎｏｄｏｎｔｉｕｍ ａｓｐｅｒｕｌｕｍ），ユミゴケ（Ｄｉｃｒａｎｏｄｏｎｔｉｕｍ ｄｅｎｕｄａｔｕｍ），ミヤマユミゴケ（Ｄｉｃｒａｎｏｄｏｎｔｉｕｍ ｄｉｄｉｃｔｙｏｎ），ヘリトリシッポゴケ（Ｄｉｃｒａｎｏｄｏｎｔｉｕｍ ｆｌｅｉｓｃｈｅｒｉａｎａ），タカクマユミゴケ（Ｄｉｃｒａｎｏｄｏｎｔｉｕｍ ｔｅｎｕｉｎｅｒｖｅ）などが知られている。ヘリトリシッポゴケ属の植物としては、ミヤマシッポゴケ（Ｄｉｃｒａｎｏｌｏｍａ ｃｙｌｉｎｄｒｏｔｈｅｃｉｕｍ），チョクミシッポゴケ（Ｄｉｃｒａｎｏｌｏｍａ ｃｙｌｉｎｄｒｏｔｈｅｃｉｕｍ ｖａｒ． ｂｒａｃｈｙｃａｒｐｕｍ），ナガバシッポゴケ（Ｄｉｃｒａｎｏｌｏｍａ ｃｙｌｉｎｄｒｏｔｈｅｃｉｕｍ ｖａｒ． ｍａｅｄａｅ）などが知られている。オウギゴケ属の植物としては、オウギゴケ（Ｄｉｃｒａｎｏｗｅｉｓｉａ ｃｒｉｓｐｕｌａ）などが知られている。シッポゴケ属の植物としては、ヌマシッポゴケ（Ｄｉｃｒａｎｕｍ ｂｏｎｊｅａｎｉｉ），アオシッポゴケ（Ｄｉｃｒａｎｕｍ ｃａｅｓｉｕｍ），デワシッポゴケ（Ｄｉｃｒａｎｕｍ ｄｒｕｍｍｏｎｄｉｉ），ユキミシッポゴケ（Ｄｉｃｒａｎｕｍ ｅｌｏｎｇａｔｕｍ），ヒメカモジゴケ（Ｄｉｃｒａｎｕｍ ｆｌａｇｅｌｌａｒｅ），フジシッポゴケ（Ｄｉｃｒａｎｕｍ ｆｕｌｖｕｍ），チャシッポゴケ（Ｄｉｃｒａｎｕｍ ｆｕｓｃｅｓｃｅｎｓ），ゴウノシッポゴケ（Ｄｉｃｒａｎｕｍ ｇｏｎｏｉ），カラフトシッポゴケ（Ｄｉｃｒａｎｕｍ ｇｒｏｅｎｌａｎｄｉｃｕｍ），カギカモジゴケ（Ｄｉｃｒａｎｕｍ ｈａｍｕｌｏｓｕｍ），シッポゴケ（Ｄｉｃｒａｎｕｍ ｊａｐｏｎｉｃｕｍ），シワシッポゴケ（Ｄｉｃｒａｎｕｍ ｊａｐｏｎｉｃｕｍ ｖａｒ． ｒｕｇｕｌｏｓｕｍ），ナスシッポゴケ（Ｄｉｃｒａｎｕｍ ｌｅｉｏｄｏｎｔｕｍ），チシマシッポゴケ（Ｄｉｃｒａｎｕｍ ｍａｊｕｓ），コカモジゴケ（Ｄｉｃｒａｎｕｍ ｍａｙｒｉｉ），タツナミカマシッポゴケ（Ｄｉｃｒａｎｕｍ ｍｕｅｈｌｅｎｂｅｃｋｉｉ），オオシッポゴケ（Ｄｉｃｒａｎｕｍ ｎｉｐｐｏｎｅｎｓｅ），ナミシッポゴケ（Ｄｉｃｒａｎｕｍ ｐｏｌｙｓｅｔｕｍ），コクロベシッポゴケ（Ｄｉｃｒａｎｕｍ ｓａｓａｏｋａｅ），カモジゴケ（Ｄｉｃｒａｎｕｍ ｓｃｏｐａｒｉｕｍ），オニカモジゴケ（Ｄｉｃｒａｎｕｍ ｓｃｏｐａｒｉｕｍ），イシヅチカモジゴケ（Ｄｉｃｒａｎｕｍ ｓｃｏｐａｒｉｕｍ ｖａｒ． ｏｒｔｈｏｃａｒｐｕｍ），ケシッポゴケ（Ｄｉｃｒａｎｕｍ ｓｅｔｉｆｏｌｉｕｍ），ヤマシッポゴケ（Ｄｉｃｒａｎｕｍ ｓｙｍｂｌｅｐｈａｒｉｏｉｄｅｓ），ナガエノシッポゴケ（Ｄｉｃｒａｎｕｍ ｕｎｄｕｌａｔｕｍ），タカネシッポゴケ（Ｄｉｃｒａｎｕｍ ｖｉｒｉｄｅ ｖａｒ． ｈａｋｋｏｄｅｎｓｅ）などが知られている。ニセタマウケゴケ属の植物としては、ニセタマウケゴケ（Ｇａｒｃｋｅａ ｆｌｅｘｕｏｓａ）などが知られている。マイマイゴケ属の植物としては、マイマイゴケ（Ｈｏｌｏｍｉｔｒｉｕｍ ｄｅｎｓｉｆｏｌｉｕｍ）などが知られている。カマシッポゴケ属の植物としては、アオジクカマシッポゴケ（Ｋｉａｅｒｉａ ｂｌｙｔｉｉ），カマシッポゴケ（Ｋｉａｅｒｉａ ｆａｌｃａｔａ），ノコギリカマシッポゴケ（Ｋｉａｅｒｉａ ｆａｌｃａｔａ ｖａｒ． ｓｅｒｒａｔｉｆｏｌｉａ），アカジクカマシッポゴケ（Ｋｉａｅｒｉａ ｓｔａｒｋｅｉ）などが知られている。マツバゴケ属の植物としては、マツバゴケ（Ｌｅｕｃｏｌｏｍａ ｍｏｌｌｅ），イボマツバゴケ（Ｌｅｕｃｏｌｏｍａ ｏｋａｍｕｒａｅ）などが知られている。コブゴケ属の植物としては、チジミバコブゴケ（Ｏｎｃｈｏｐｈｏｒｕｓ ｃｒｉｓｐｉｆｏｌｉｕｓ），コエノコブゴケ（Ｏｎｃｈｏｐｈｏｒｕｓ ｃｒｉｓｐｉｆｏｌｉｕｓ ｖａｒ． ｂｒｅｖｉｐｅｓ），オオコブゴケ（Ｏｎｃｈｏｐｈｏｒｕｓ ｖｉｒｅｎｓ），ノコギリゴケ（Ｏｎｃｈｏｐｈｏｒｕｓ ｖｉｒｅｎｓ ｖａｒ． ｓｅｒｒａｔｕｓ），エゾノコブゴケ（Ｏｎｃｈｏｐｈｏｒｕｓ ｗａｈｌｅｎｂｅｒｇｉｉ）などが知られている。ヤマゴケ属の植物としては、ヤマゴケ（Ｏｒｅａｓ ｍａｒｔｉａｎａ）などが知られている。ミヤマゴケ属の植物としては、タカネセンボンゴケ（Ｏｒｅｏｗｅｉｓｉａ ｌａｘｉｆｏｌｉａ）などが知られている。ナガバノシッポゴケ属の植物としては、フトスジニセオキナゴケ（Ｐａｒａｌｅｕｃｏｂｒｙｕｍ ｅｎｅｒｖｅ），ナガバノシッポゴケ（Ｐａｒａｌｅｕｃｏｂｒｙｕｍ ｌｏｎｇｉｆｏｌｉｕｍ）などが知られている。ヤスジゴケ属の植物としては、ナメハヤスジゴケ（Ｒｈａｂｄｏｗｅｉｓｉａ ｃｒｉｓｐａｔａ）などが知られている。ナガダイゴケ属の植物としては、キンシナガダイゴケ（Ｔｒｅｍａｔｏｄｏｎ ａｍｂｉｇｕｏｕｓ），アカマルゴケ（Ｔｒｅｍａｔｏｄｏｎ ｂｒｅｖｉｃａｒｐｕｓ），ハクサンナガダイゴケ（Ｔｒｅｍａｔｏｄｏｎ
ｈａｋｕｓａｎｅｎｓｉｓ），ユミダイゴケ（Ｔｒｅｍａｔｏｄｏｎ ｌｏｎｇｉｃｏｌｌｉｓ），マエバラナガダイゴケ（Ｔｒｅｍａｔｏｄｏｎ ｍａｙｅｂａｒａｅ），シマオバナゴケ（Ｔｒｅｍａｔｏｄｏｎ ｓｅｍｉｔｏｒｔｉｄｅｎｓ）などが知られている。
The plant used as a raw material of this invention should just be a plant of the Diploceae family ( Dicranaceae ). Examples of the plant belonging to the genus A. cerevisiae ( Angstroemia ), the genus Hippogoke ( Arctoa ), the genus Sphagnum ( Brothera ), the genus Bruchia , the genus Broughumbeti ( Bryohumberi ) , Inunohagoke genus (Cynodontium), Kumadegoke genus (Dichodontium), Susukigoke genus (Dicranella), Yumigoke genus (Dicranodontium), helicopter tri Shippogoke genus (Dicranoloma), Ougigoke genus (Dicranoweisia), Shippogoke genus (Dicranum), Nisetamaukegoke genus (Garckea) , Maimaigo (genus Holomitrium), sickle Shippogoke genus (Kiaeria), Matsubagoke genus (Leucoloma), Kobugoke genus (Onchophorus), Yamagoke genus (Oreas), Miyamagoke genus (Oreoweisia), Naga bus Roh Shippogoke genus (Paraleucobryum), Yasujigoke genus (Rhabdoweisia), Nagadaigoke More than 50 genera such as the genus ( Trematodon ) are known. As a plant belonging to the genus Hatagogoke , there is known, for example, Aungstroemeria juracea . The Kishippogoke plants of the genus, Kishippogoke (Arctoa fulvella), Naga Exhibition Shippogoke (Arctoa fulvella var. Longisetacea), etc. Takanekamojigoke (Arctoa schistioides) is known. As a plant belonging to the genus Shimosoke, Shimosoke ( Brothera leana ) and the like are known. As a plant belonging to the genus Bruchogoke, Bruchogoke ( Bruchia microspora ) and the like are known. As a plant belonging to the genus Thorivarietomodomo , there is known Bryohumbertia subcomosa . As plants belonging to the genus Snakego, snake moss ( Campypodium medium ) and the like are known. The Tsuribarigoke plants of the genus, Kurotsuribarigoke (Campylopus atrovirens), Mayuhakegoke (Campylopus fragilis), Yamatofudegoke (Campylopus japonicus), Hiro streaks tree burr moss (Campylopus schwarzii), Fudegoke (Campylopus umbellatus), for about Matsuri burr moss (Campylopus yakushimensis) etc. It has been known. The Inunohagoke plants of the genus, Okainunohagoke (Cynodontium fallax), Miyama dog Noja moss (Cynodontium gracilescens), Inunohagoke (Cynodontium polycarpum), Kobugoke (Cynodontium strumiferum), etc. Himeinunohagoke (Cynodontium tenellum) is known. The Kumadegoke plants of the genus, Shimeriiwagoke (Dichodontium pellucidum), Kumadegoke (Dichodontium pellucidum var. Japonicum), Ezokumadegoke (Dichodontium pellucidum var. Yezoense), etc. Iboiwagoke (Dichodontium verrucosum) is known. The Susukigoke genus plant, Aomori sinensis moss (Dicranella brachyangia), Kobuobanagoke (Dicranella cerviculata), Horai male moss (Dicranella coarctata), Michino Kuo nosed moss (Dicranella dilatatinervis), Kinshigokemodoki (Dicranella ditrichoides), Tamasusukigoke (Dicranella globuligera), Iyosusukigoke (Dicranella gonoi), Susukigoke (Dicranella heteromalla), Yugamisusukigoke (Dicranella heteromalla var. curvipes), Kosusukigoke (Dicranell iisibae), Shimasusukigoke (Dicranella javanica), horsetail pampas grass moss (Dicranella kiushiana), Tsukushihanagagoke (Dicranella mayebarae), Oshima pampas grass moss (Dicranella oshimae), Hirohasusukigoke (Dicranella palustris), Ezonoobanagoke (Dicranella schreberiana), Miyama pampas grass moss (Dicranella subsecunda) , Calaftus skigoke ( Dicranella subulata ), Nagasjisukigoke ( Diclanella varia ), Ezosukisokego ( Dicranella yezoana ), etc. are known. The Yumigoke plants of the genus, Katabayumigoke (Dicranodontium asperulum), Yumigoke (Dicranodontium denudatum), Miyamayumigoke (Dicranodontium didictyon), helicopter tri Shippogoke (Dicranodontium fleischeriana), etc. Takakumayumigoke (Dicranodontium tenuinerve) is known. The helicopter birds Shippogoke plants of the genus, Miyama Shippogoke (Dicranoloma cylindrothecium), Cho Kumi Shippogoke (Dicranoloma cylindrothecium var. Brachycarpum), Naga bar Shippogoke (Dicranoloma cylindrothecium var. Maedae) and the like are known. As a plant belonging to the genus Astragalus, Astragalus ( Dicranouisia crispula ) and the like are known. The Shippogoke plants of the genus, Numa Shippogoke (Dicranum bonjeanii), Blue Shippogoke (Dicranum caesium), Dewashippogoke (Dicranum drummondii), Yuki Mi Shippogoke (Dicranum elongatum), Himekamojigoke (Dicranum flagellare), Fuji Shippogoke (Dicranum fulvum), tea Shippogoke ( Dicranum fuscescens), Gow Roh Shippogoke (Dicranum gonoi), Sakhalin Shippogoke (Dicranum groenlandicum), Kagikamojigoke (Dicranum hamulosum), Shippogoke (Dicranum japonicum), wrinkles Shippogoke (Dic ranum japonicum var. rugulosum), eggplant Shippogoke (Dicranum leiodontum), Chishima Shippogoke (Dicranum majus), Kokamojigoke (Dicranum mayrii), data tsunami sickle Shippogoke (Dicranum muehlenbeckii), giant Shippogoke (Dicranum nipponense), Nami Shippogoke (Dicranum polysetum), co arborvitae Shippogoke (Dicranum sasaokae), Kamojigoke (Dicranum scoparium), Onikamojigoke (Dicranum scoparium), Ishizu Chika Moji moss (Dicranum scoparium var. orthocarpum), Keshi' Moss (Dicranum setifolium), mountain Shippogoke (Dicranum symblepharioides), Nagara Roh Shippogoke (Dicranum undulatum), Takamine Shippogoke (Dicranum viride var. Hakkodense) and the like are known. As a plant belonging to the genus Nisetama pokeweed , there is known, for example, Necka pokeweed ( Garcea flexuosa ). As a plant belonging to the genus Maimaigo, Maimaioke ( Horomitrium densifolium ) and the like are known. The Kama Shippogoke plants in the genus Emberiza click Kama Shippogoke (Kiaeria blytii), sickle Shippogoke (Kiaeria falcata), saw Kama Shippogoke (Kiaeria falcata var. Serratifolia), such as red Axis sickle Shippogoke (Kiaeria starkei) is known. As plants belonging to the genus Matsubago , there are known matsubago ( Leucoloma molle ), Iburomatsubago ( Leucoloma okamurae ) and the like. The Kobugoke plants of the genus, Chijimibakobugoke (Onchophorus crispifolius), Koenokobugoke (Onchophorus crispifolius var. Brevipes), Ookobugoke (Onchophorus virens), Nokogirigoke (Onchophorus virens var. Serratus), such Ezonokobugoke (Onchophorus wahlenbergii) is known. As a plant belonging to the genus Corydalis, for example, Corydalis ( Oreas martiana ) is known. As a plant belonging to the genus Mayamago, Takanesenbongoke ( Oreowisia laxifolia ) and the like are known. Examples of plants belonging to the genus Nagabano sipogoke are Parascobrium enerve , Paraleucobryum longifolium, and the like. As a plant belonging to the genus Yasujigoke, Rhabdoweisia crispata and the like are known. The Nagadaigoke genus plant, gold Naga die Moss (Trematodon ambiguous), Akamarugoke (Trematodon brevicarpus), Hakusan Naga die moss (Trematodon
Hakusanensis ), Yumidaigoke ( Trematodon longicollis ), Maebaranagaigoke ( Trematodon mayebarae ), Timadonagoke ( Trematodon seminaritides ), etc. are known.
本発明に用いられる原料となる植物は、シッポゴケ科植物であれば特に限定されないが、入手が比較的容易なことや有効性などの理由から、シッポゴケ属（Ｄｉｃｒａｎｕｍ）植物，ツリバリゴケ属（Ｃａｍｐｙｌｏｐｕｓ）植物を好適に用いることが出来る。シッポゴケ属（Ｄｉｃｒａｎｕｍ）植物，ツリバリゴケ属（Ｃａｍｐｙｌｏｐｕｓ）植物としては、シッポゴケ（Ｄｉｃｒａｎｕｍ ｊａｐｏｎｉｃｕｍ），カモジゴケ（Ｄｉｃｒａｎｕｍ ｓｃｏｐａｒｉｕｍ），フデゴケ（Ｃａｍｐｙｌｏｐｕｓ ｕｍｂｅｌｌａｔｕｓ）を用いることが好ましく、シッポゴケを用いることが有効性の点から特に好ましい。 Plants as a raw material used in the present invention is not particularly limited as long as Shippogoke family plant, because such availability is relatively easy for and effectiveness, Shippogoke genus (Dicranum) plants Tsuribarigoke genus (Campylopus) Plants Can be suitably used. Shippogoke genus (Dicranum) plants, the Tsuribarigoke genus (Campylopus) plants, Shippogoke (Dicranum japonicum), Kamojigoke (Dicranum scoparium), it is preferable to use a Fudegoke (Campylopus umbellatus), in particular in terms of possible efficacy using Shippogoke preferable.
これらシッポゴケ科植物を使用する際は、抽出物を用いるのが一般的である。抽出には、シッポゴケ科植物の胞子，胞子体，配偶体のいずれの部位を用いても構わないが、簡便に利用するには、胞子体と配偶体の全草を用いるとよい。抽出の際は、生のまま用いてもよいが、抽出効率を考えると、細切，乾燥，粉砕等の処理を行った後に抽出を行うことが好ましい。抽出は、抽出溶媒に浸漬するか、超臨界流体や亜臨界流体を用いた抽出方法でも行うことができる。抽出効率を上げるため、撹拌や抽出溶媒中でホモジナイズしてもよい。抽出温度としては、５℃程度から抽出溶媒の沸点以下の温度とするのが適切である。抽出時間は抽出溶媒の種類や抽出温度によっても異なるが、１時間〜１４日間程度とするのが適切である。 When using these plant species, it is common to use an extract. For extraction, any of the spores, spore bodies, and gametophytes of the genus Schipogokeaceae may be used, but for easy use, the whole plant of spore bodies and gametophytes may be used. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days.
抽出溶媒としては、水の他、メタノール，エタノール，プロパノール，イソプロパノール等の低級アルコール、１，３−ブチレングリコール，プロピレングリコール，ジプロピレングリコール，グリセリン等の多価アルコール、エチルエーテル，プロピルエーテル等のエーテル類、酢酸ブチル，酢酸エチル等のエステル類、アセトン，エチルメチルケトン等のケトン類などの溶媒を用いることができ、これらより１種又は２種以上を選択して用いる。また、生理食塩水，リン酸緩衝液，リン酸緩衝生理食塩水等を用いてもよい。さらに、水や二酸化炭素，エチレン，プロピレン，エタノール，メタノール，アンモニアなどの１種又は２種以上の超臨界流体や亜臨界流体を用いてもよい。 Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, and ethers such as ethyl ether and propyl ether. , Solvents such as esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these are selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.
シッポゴケ科植物の上記溶媒による抽出物は、そのままでも使用することができるが、濃縮，乾固した物を水や極性溶媒に再度溶解したり、或いはこれらの生理作用を損なわない範囲で脱色，脱臭，脱塩等の精製処理を行ったり、カラムクロマトグラフィー等による分画処理を行った後に用いてもよい。シッポゴケ科植物の前記抽出物やその処理物及び分画物は、各処理及び分画後に凍結乾燥し、用時に溶媒に溶解して用いることもできる。 The above-mentioned extract of the plant belonging to the plant belonging to the genus Schipogokeaceae can be used as it is, but it can be decolorized and deodorized as long as the concentrated and dried product is dissolved again in water or a polar solvent, or the physiological action thereof is not impaired. It may be used after a purification treatment such as desalting or a fractionation treatment by column chromatography or the like. The above-mentioned extract of the plant belonging to the genus Schipogokeaceae, its processed products and fractions can be freeze-dried after each treatment and fractionation and dissolved in a solvent before use.
シッポゴケ科植物の抽出物は、優れた細胞賦活作用、ヒアルロン酸産生促進作用、デコリン産生促進作用、抗酸化作用を有し、細胞賦活剤、ヒアルロン酸産生促進剤、デコリン産生促進剤、抗酸化剤として利用することができる。 The extract of the plant belonging to the family Schipogokeaceae has excellent cell activation action, hyaluronic acid production promotion action, decorin production promotion action, antioxidant action, cell activator, hyaluronic acid production promotion agent, decorin production promotion agent, antioxidant agent Can be used as
シッポゴケ科植物の抽出物を有効成分とする細胞賦活剤は、種々の細胞に対して優れた賦活作用を発揮するが、特に真皮線維芽細胞に対して優れた効果を発揮する。 A cell activator comprising an extract of a plant belonging to the genus Schipogokeaceae as an active ingredient exerts an excellent activation action on various cells, but particularly exhibits an excellent effect on dermal fibroblasts.
シッポゴケ科植物の抽出物を有効成分とするヒアルロン酸産生促進剤、デコリン産生促進剤は、真皮マトリックス成分の産生促進に優れた効果を発揮し、ヒアルロン酸やデコリンの産生促進に優れた効果を発揮する。 Hyaluronic acid production promoter and decorin production promoter, which uses an extract from the plant of Chrysophaceae, as an active ingredient, has an excellent effect in promoting the production of dermal matrix components, and an excellent effect in promoting the production of hyaluronic acid and decorin. To do.
シッポゴケ科植物の抽出物を有効成分とする抗酸化剤は、優れた抗酸化作用を発揮するが、特にフリーラジカル消去作用に優れた効果を発揮する。 An antioxidant comprising an extract of a plant belonging to the genus Schipogokeaceae as an active ingredient exhibits an excellent antioxidant action, but particularly exhibits an excellent effect on free radical scavenging action.
また、シッポゴケ科植物の抽出物を皮膚外用剤に配合することにより、皮膚老化症状の防止・改善に優れた効果を発揮する老化防止改善用の皮膚外用剤を得ることができる。さらに、シッポゴケ科植物の抽出物は、健康維持や栄養補給を目的とするような食品や飲料にも用いることもできる。 Moreover, the skin external preparation for anti-aging improvement which exhibits the effect excellent in prevention and improvement of a skin aging symptom can be obtained by mix | blending the extract of a plant of the genus Hippophyceae with the external preparation for skin. Furthermore, the extract of the plant belonging to the family Schipogokeaceae can also be used in foods and beverages for the purpose of maintaining health and providing nutrition.
シッポゴケ科植物の抽出物を皮膚外用剤に配合する際の配合量は、皮膚外用剤の種類や使用目的等によって調整することができるが、効果や安定性などの点から、全量に対して、０．０００１〜５０．０重量％が好ましく、より好ましくは、０．００１〜１０．０重量％である。 The blending amount of the extract of the plant belonging to the plant family can be adjusted according to the type of skin external preparation and the purpose of use, etc., but from the point of effect and stability, 0.0001-50.0 weight% is preferable, More preferably, it is 0.001-10.0 weight%.
シッポゴケ科植物の抽出物を配合する皮膚外用剤の剤型は任意であり、例えば、ローションなどの可溶化系、クリームや乳液などの乳化系，カラミンローション等の分散系として提供することができる。さらに、噴射剤と共に充填したエアゾール，軟膏剤，粉末，顆粒などの種々の剤型で提供することもできる。 The dosage form of the external preparation for skin containing the extract of the plant belonging to the genus Schipogokeaceae is arbitrary. For example, it can be provided as a solubilizing system such as lotion, an emulsifying system such as cream or emulsion, or a dispersing system such as calamine lotion. Furthermore, it can also be provided in various dosage forms such as aerosols, ointments, powders and granules filled with a propellant.
なお、シッポゴケ科植物の抽出物を配合する皮膚外用剤には、シッポゴケ科植物の抽出物の他に、必要に応じて、通常医薬品，医薬部外品，皮膚化粧料，毛髪用化粧料及び洗浄料に配合される、油性成分，保湿剤，粉体，色素，乳化剤，可溶化剤，洗浄剤，紫外線吸収剤，増粘剤，薬剤，香料，樹脂，防菌防黴剤，アルコール類等を適宜配合することができる。また、本発明の効果を損なわない範囲において、他の細胞賦活剤、ヒアルロン酸産生促進剤、デコリン産生促進剤、抗酸化剤との併用も可能である。 In addition, for the topical skin preparation containing an extract of the plant belonging to the family Schipogokeaceae, in addition to the extract from the plant belonging to the family Schipogokeaceae, if necessary, it is usually a pharmaceutical, a quasi-drug, a skin cosmetic, a cosmetic for hair and a washing. Oil components, moisturizers, powders, pigments, emulsifiers, solubilizers, cleaning agents, UV absorbers, thickeners, drugs, fragrances, resins, antibacterial and antifungal agents, alcohols, etc. It can mix | blend suitably. Moreover, in the range which does not impair the effect of this invention, combined use with another cell activator, a hyaluronic acid production promoter, a decorin production promoter, and an antioxidant is also possible.
以下に、シッポゴケ科植物の抽出物の製造例、各作用を評価するための試験、皮膚外用剤としての処方例、使用試験についてさらに詳細に説明するが、本発明の技術的範囲はこれによってなんら限定されるものではない。 In the following, production examples of extracts of the plant belonging to the genus Schipogokeaceae, tests for evaluating each action, formulation examples as external preparations for skin, and use tests will be described in more detail, but the technical scope of the present invention is not limited thereby. It is not limited.
［製造例１］
シッポゴケ科植物の全草の乾燥粉砕物１ｋｇに５０重量％エタノール水溶液を１０リットル加え、室温で７日間浸漬した。抽出液をろ過して回収し、溶媒を除去した後、シッポゴケ科植物抽出物を得た。
[Production Example 1]
10 kg of 50% by weight aqueous ethanol solution was added to 1 kg of dried pulverized whole plant of Hippoceae and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, an extract of a plant belonging to the family Schipogoke was obtained.
［製造例２］
シッポゴケ科植物の全草の乾燥粉砕物１ｋｇに水を９リットル加え、９０℃にて６時間還流して抽出した。抽出液をろ過して回収し、溶媒を除去した後、シッポゴケ科植物抽出物を得た。
[Production Example 2]
Nine liters of water was added to 1 kg of a dry pulverized whole plant of the genus Schipogokeaceae and extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration, and after removing the solvent, an extract of a plant belonging to the family Schipogoke was obtained.
［製造例３］
シッポゴケ科植物の全草の乾燥粉砕物１ｋｇにメタノールを９リットル加え、室温で７日間浸漬した。抽出液をろ過して回収し、溶媒を除去した後、シッポゴケ科植物抽出物を得た。
[Production Example 3]
Nine liters of methanol was added to 1 kg of a dry pulverized whole plant of Hippoceae and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, an extract of a plant belonging to the family Schipogoke was obtained.
［製造例４］
超臨界抽出装置にシッポゴケ科植物の全草を投入し、４０℃において１５ＭＰａの気圧下で二酸化炭素の超臨界流体を用いて抽出した。抽出物を回収し、シッポゴケ科植物抽出物を得た。
[Production Example 4]
The whole plant of the plant belonging to the family Schippoaceae was put into a supercritical extraction apparatus and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under a pressure of 15 MPa. The extract was collected to obtain an extract of the plant belonging to the family Schipogokeaceae.
次に、シッポゴケ科植物抽出物の真皮線維芽細胞の賦活作用について示す。試料には、シッポゴケより製造例１を用いて抽出したシッポゴケ抽出物を用いた。 Next, it shows about the activation effect | action of the dermis fibroblast of a plant of the genus Schipogokeaceae. The sample used was a shippogoch extract extracted from shippogoke using Production Example 1.
評価は、以下の手順で行った。正常ヒト真皮線維芽細胞を１ウェル当たり２．０×１０４個となるように９６穴マイクロプレートに播種した。播種培地には、ダルベッコ改変イーグル培地（ＤＭＥＭ）に１％のウシ胎児血清を添加したものを用いた。２４時間培養後、任意の濃度の試料を添加した試験培地に交換し、さらに４８時間培養した。次いで３−(４,５−ジメチル−２−チアゾリル)−２,５−ジフェニルテトラゾリウムブロミド（ＭＴＴ）を４００μｇ／ｍＬ含有する培地に交換して２時間培養し、テトラゾリウム環の開環により生じるフォルマザンを２−プロパノールにて抽出し、マイクロプレートリーダーにて５５０ｎｍの吸光度を測定した。同時に濁度として６５０ｎｍにおける吸光度を測定し、両測定値の差により細胞賦活作用を評価した。評価結果を、試料無添加のブランクにおける細胞賦活作用を１００とした相対値にて表１に示す。なお、表中の*及び**は、ｔ検定における有意確率Ｐ値に対し、有意確率５％未満（Ｐ＜０．０５）を*で、有意確率１％未満（Ｐ＜０．０１）を**で表したものである。 The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 4 cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum. After culturing for 24 hours, the culture medium was replaced with a test medium to which a sample having an arbitrary concentration was added, and further cultured for 48 hours. Next, the medium containing 400 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was exchanged and cultured for 2 hours. Formazan produced by the opening of the tetrazolium ring was removed. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in Table 1 as relative values with the cell activation effect in the blank with no sample as 100. In the table, * and ** indicate a significance probability of less than 5% (P <0.05) with respect to the significance probability P value in the t test, and a significance probability of less than 1% (P <0.01). It is represented by **.
表１より明らかなように、シッポゴケ抽出物を添加した培地では、有意な真皮線維芽細胞賦活作用が認められた。特に、シッポゴケ抽出物を０．２５〜０．５ｍｇ／ｍＬ添加した場合には、ブランクと比較して、危険率１％未満で有意な真皮線維芽細胞賦活作用が認められた。このことから、シッポゴケ抽出物は、優れた真皮線維芽細胞賦活作用を有することが明らかとなった。 As is clear from Table 1, a significant dermal fibroblast activation effect was observed in the medium supplemented with the Shippogoke extract. In particular, when 0.25-0.5 mg / mL of the Sipogoke extract was added, a significant dermal fibroblast activation effect was observed at a risk rate of less than 1% compared to the blank. From this, it has been clarified that the extract of Shippogoke has an excellent dermal fibroblast activation effect.
次に、シッポゴケ科植物抽出物のヒアルロン酸産生促進作用の評価を示す。試料には、シッポゴケより製造例１を用いて抽出したシッポゴケ抽出物を用いた。 Next, evaluation of hyaluronic acid production promoting action of the plant belonging to the plant family plant is shown. The sample used was a shippogoch extract extracted from shippogoke using Production Example 1.
評価は、以下の手順で行った。正常ヒト真皮線維芽細胞を１ウェル当たり２．０×１０４個となるように９６穴マイクロプレートに播種し、任意の濃度の試料を添加した０．５重量％牛胎仔血清添加ダルベッコ修正基礎培地（ＤＭＥＭ）にて３７℃で５日間培養し、培養上清のヒアルロン酸量をＥｎｚｙｍｅ−ｌｉｎｋｅｄ ｉｍｍｕｎｏｓｏｒｂｅｎｔ ａｓｓａｙ（ＥＬＩＳＡ）法により測定した。同時に線維芽細胞数を計測し、細胞当たりのヒアルロン酸産生量を算出して、試料を含有しないブランクの細胞当たりのヒアルロン酸産生量を１００とした相対値にて表２に示した。なお、表中の*及び**は、ｔ検定における有意確率Ｐ値に対し、有意確率５％未満（Ｐ＜０．０５）を*で、有意確率１％未満（Ｐ＜０．０１）を**で表したものである。 The evaluation was performed according to the following procedure. Dulbecco's modified basal medium supplemented with 0.5% by weight fetal calf serum, in which normal human dermal fibroblasts are seeded in a 96-well microplate so as to be 2.0 × 10 4 per well, and a sample of any concentration is added (DMEM) was cultured at 37 ° C. for 5 days, and the amount of hyaluronic acid in the culture supernatant was measured by the enzyme-linked immunosorbent assay (ELISA) method. At the same time, the number of fibroblasts was counted, the amount of hyaluronic acid produced per cell was calculated, and the relative value with the amount of hyaluronic acid produced per blank cell containing no sample as 100 was shown in Table 2. In the table, * and ** indicate a significance probability of less than 5% (P <0.05) with respect to the significance probability P value in the t test, and a significance probability of less than 1% (P <0.01). It is represented by **.
表２より明らかなように、シッポゴケ抽出物を添加した場合に、未添加の場合と比較して、有意なヒアルロン酸産生促進作用が認められた。特に、シッポゴケ抽出物を７．８〜６２．５μｇ/ｍＬ添加した場合には、危険率１％未満で有意なヒアルロン酸産生促進作用が認められた。このことから、シッポゴケ抽出物は、優れたヒアルロン酸産生促進作用を有することが明らかとなった。 As can be seen from Table 2, when the Shippogoke extract was added, a significant hyaluronic acid production promoting effect was observed as compared with the case where it was not added. In particular, when 7.8-62.5 μg / mL of the syrup moss extract was added, a significant hyaluronic acid production promoting effect was observed at a risk rate of less than 1%. From this, it has been clarified that the syrup moss extract has an excellent hyaluronic acid production promoting action.
次に、シッポゴケ科植物抽出物のデコリン産生促進作用の評価を示す。試料には、シッポゴケより製造例１を用いて抽出したシッポゴケ抽出物を用いた。 Next, the evaluation of the decorin production promoting action of the plant plant extract is shown. The sample used was a shippogoch extract extracted from shippogoke using Production Example 1.
評価は、以下の手順で行った。正常ヒト真皮線維芽細胞を１ウェル当たり２．０×１０４個となるように９６穴マイクロプレートに播種し、試料を任意の濃度添加した１．０重量％牛胎仔血清添加ダルベッコ修正基礎培地（ＤＭＥＭ）にて３７℃で２４時間培養し、培養上清のデコリン量をＥＬＩＳＡ法により測定した。同時に線維芽細胞数を計測し、細胞当たりのデコリン産生量を算出して、試料を含有しないブランクの細胞当たりのデコリン産生量を１００とした相対値にて表３に示した。なお、表中の*及び**は、ｔ検定における有意確率Ｐ値に対し、有意確率５％未満（Ｐ＜０．０５）を*で、有意確率１％未満（Ｐ＜０．０１）を**で表したものである。 The evaluation was performed according to the following procedure. Normal human dermal fibroblasts are seeded in a 96-well microplate at 2.0 × 10 4 cells per well, and 1.0 wt% fetal bovine serum-added Dulbecco's basal medium supplemented with an arbitrary concentration of sample ( DMEM) was cultured at 37 ° C. for 24 hours, and the amount of decorin in the culture supernatant was measured by ELISA. At the same time, the number of fibroblasts was counted, the amount of decorin produced per cell was calculated, and the relative value with the amount of decorin produced per blank cell containing no sample as 100 was shown in Table 3. In the table, * and ** indicate a significance probability of less than 5% (P <0.05) with respect to the significance probability P value in the t test, and a significance probability of less than 1% (P <0.01). It is represented by **.
表３より明らかなように、シッポゴケ抽出物を添加した場合に、未添加の場合と比較して、有意なデコリン産生促進作用が認められた。特に、シッポゴケ抽出物を０．１３〜０．５ｍｇ/ｍＬ添加した場合には、危険率１％未満で有意なデコリン産生促進作用が認められた。このことから、シッポゴケ抽出物は、優れたデコリン産生促進作用を有することが明らかとなった。 As is clear from Table 3, a significant decorin production-promoting effect was observed when the Shippogoke extract was added compared to the case where it was not added. In particular, when 0.13 to 0.5 mg / mL of the syrup moss extract was added, a significant decorin production promoting effect was observed at a risk rate of less than 1%. From this, it has been clarified that the extract of Shippogoke has an excellent decorin production promoting action.
次に、シッポゴケ科植物抽出物の抗酸化作用について示す。試料には、シッポゴケより製造例１を用いて抽出したシッポゴケ抽出物を用いた。 Next, it shows about the antioxidant effect of a plant of the genus Schipogokeaceae. The sample used was a shippogoch extract extracted from shippogoke using Production Example 1.
評価は、以下の手順で行った。５０重量％エタノール水溶液にて任意の濃度に希釈したシッポゴケ抽出物溶液を９６穴マイクロプレートに１００μＬ添加した。次に、０．２ｍＭの濃度になるようにエタノールにて調製した１，１−ジフェニル−２−ピクリルヒドラジル（ＤＰＰＨ）溶液を９６穴マイクロプレートに１００μＬ添加した。攪拌しながら暗所に放置し、２４時間後に５１６ｎｍの吸光度を測定した。試料が無添加のブランクの吸光度を（Ａ）、試料を添加したときの吸光度を（Ｂ）としたとき、式（１）の値をラジカル消去率とした。評価結果を表４に示した。
式（１） ｛１−（Ｂ）／（Ａ）｝×１００（％）
The evaluation was performed according to the following procedure. 100 μL of a syrup moss extract solution diluted to an arbitrary concentration with a 50 wt% aqueous ethanol solution was added to a 96-well microplate. Next, 100 μL of a 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared with ethanol to a concentration of 0.2 mM was added to a 96-well microplate. The mixture was left in the dark with stirring, and the absorbance at 516 nm was measured after 24 hours. When the absorbance of the blank with no sample added was (A) and the absorbance when the sample was added was (B), the value of formula (1) was defined as the radical elimination rate. The evaluation results are shown in Table 4.
Formula (1) {1- (B) / (A)} × 100 (%)
表４より明らかなように、シッポゴケ抽出物は抗酸化作用を有することが分かった。 As is clear from Table 4, it was found that the extract of Shippogoke has an antioxidant effect.
続いて、本発明に係るシッポゴケ科植物抽出物を配合した皮膚外用剤の処方例を示す。 Then, the prescription example of the skin external preparation which mix | blended the Schipogokeaceae plant extract which concerns on this invention is shown.
［処方例１］乳液
（１）スクワラン １０．０（重量％）
（２）メチルフェニルポリシロキサン ４．０
（３）水素添加パーム核油 ０．５
（４）水素添加大豆リン脂質 ０．１
（５）モノステアリン酸ポリオキシエチレン
ソルビタン（２０Ｅ．Ｏ．） １．３
（６）モノステアリン酸ソルビタン １．０
（７）グリセリン ４．０
（８）パラオキシ安息香酸メチル ０．１
（９）カルボキシビニルポリマー ０．１５
（10）精製水 ５３．８５
（11）アルギニン（１重量％水溶液） ２０．０
（12）シッポゴケ抽出物［製造例１］ ５．０
製法：（１）〜（６）の油相成分を８０℃にて加熱溶解する。一方（７）〜（10）の水相成分を８０℃にて加熱溶解する。これに前記油相成分を攪拌しながら加え、ホモジナイザーにより均一に乳化する。乳化終了後、冷却を開始し、（11）と（12）を順次加え、均一に混合する。
[Formulation Example 1] Emulsion (1) Squalane 10.0 (wt%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 53.85
(11) Arginine (1 wt% aqueous solution) 20.0
(12) Shippogoke extract [Production Example 1] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix uniformly.
［処方例２］化粧水
（１）エタノール １５．０（重量％）
（２）ポリオキシエチレン（４０Ｅ．Ｏ．）硬化ヒマシ油 ０．３
（３）香料 ０．１
（４）精製水 ７８．３８
（５）クエン酸 ０．０２
（６）クエン酸ナトリウム ０．１
（７）グリセリン １．０
（８）ヒドロキシエチルセルロース ０．１
（９）シッポゴケ抽出物［製造例３］ ５．０
製法：（１）に（２）及び（３）を溶解する。溶解後、（４）〜（８）を順次添加した後、十分に攪拌し、（９）を加え、均一に混合する。
[Prescription Example 2] Lotion (1) Ethanol 15.0 (% by weight)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 78.38
(5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Shippogoke extract [Production Example 3] 5.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.
［処方例３］クリーム
（１）スクワラン １０．０（重量％）
（２）ステアリン酸 ２．０
（３）水素添加パーム核油 ０．５
（４）水素添加大豆リン脂質 ０．１
（５）セタノール ３．６
（６）親油型モノステアリン酸グリセリン ２．０
（７）グリセリン １０．０
（８）パラオキシ安息香酸メチル ０．１
（９）アルギニン（２０重量％水溶液） １５．０
（10）精製水 ４０．７
（11）カルボキシビニルポリマー（１重量％水溶液） １５．０
（12）シッポゴケ抽出物［製造例１］ １．０
製法：（１）〜（６）の油相成分を８０℃にて加熱溶解する。一方（７）〜（10）の水相成分を８０℃にて加熱溶解する。これに前記油相成分を攪拌しながら加え、ホモジナイザーにより均一に乳化する。乳化終了後、（11）を加え、冷却を開始し、４０℃にて（12）を加え、均一に混合する。
[Prescription Example 3] Cream (1) Squalane 10.0 (% by weight)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20% by weight aqueous solution) 15.0
(10) Purified water 40.7
(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Shippogoke extract [Production Example 1] 1.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification is completed, add (11), start cooling, add (12) at 40 ° C., and mix uniformly.
［処方例４］美容液
（１）精製水 ２７．４５(重量％)
（２）グリセリン １０．０
（３）ショ糖脂肪酸エステル １．３
（４）カルボキシビニルポリマー（１重量％水溶液） １７．５
（５）アルギン酸ナトリウム(１重量％水溶液) １５．０
（６）モノラウリン酸ポリグリセリル １．０
（７）マカデミアナッツ油脂肪酸フィトステリル ３．０
（８）N-ラウロイル-L-グルタミン酸
ジ（フィトステリル−２−オクチルドデシル） ２．０
（９）硬化パーム油 ２．０
（10）スクワラン（オリーブ由来） １．０
（11）ベヘニルアルコール ０．７５
（12）ミツロウ １．０
（13）ホホバ油 １．０
（14）１，３−ブチレングリコール １０．０
（15）Ｌ−アルギニン（１０重量％水溶液） ２．０
（16）シッポゴケ抽出物［製造例１］ ５．０
製法：（１）〜（６）の水相成分を混合し、７５℃にて加熱溶解する。一方、（７）〜（14）の油相成分を混合し、７５℃にて加熱溶解する。次いで、上記水相成分に油相成分を添加して予備乳化を行った後、ホモミキサーにて均一に乳化する。乳化終了後に冷却を開始し、５０℃にて（15）を加える。さらに４０℃まで冷却し、（16）を加え、均一に混合する。
[Formulation Example 4] Cosmetic liquid (1) Purified water 27.45 (% by weight)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1 wt% aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by weight aqueous solution) 2.0
(16) Shippogoke extract [Production Example 1] 5.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C, add (16) and mix evenly.
［処方例５］水性ジェル
（１）カルボキシビニルポリマー ０．５（重量％）
（２）精製水 ８６．７
（３）水酸化ナトリウム（１０重量％水溶液） ０．５
（４）エタノール １０．０
（５）パラオキシ安息香酸メチル ０．１
（６）香料 ０．１
（７）シッポゴケ抽出物［製造例４］ ２．０
（８）ポリオキシエチレン（６０Ｅ．Ｏ．）硬化ヒマシ油 ０．１
製法：（１）を（２）に加え、均一に攪拌した後、（３）を加える。均一に攪拌した後，（４）に予め溶解した（５）を加える。均一に攪拌した後、予め混合しておいた（６）〜（８）を加え、均一に攪拌混合する。
[Formulation Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (% by weight)
(2) Purified water 86.7
(3) Sodium hydroxide (10% by weight aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Shippogoke extract [Production Example 4] 2.0
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, add (5) previously dissolved in (4). After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.
［処方例６］クレンジング料
（１）スクワラン ８１．０（重量％）
（２）イソステアリン酸ポリオキシエチレングリセリル １５．０
（３）精製水 ３．０
（４）シッポゴケ抽出物［製造例４］ １．０
製法：（１）と（２）を均一に溶解する。これに、（３）と（４）を順次加え、均一に混合する。
[Formulation Example 6] Cleansing Fee (1) Squalane 81.0 (wt%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Purified water 3.0
(4) Shippogoke extract [Production Example 4] 1.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.
［処方例７］洗顔フォーム
（１）ステアリン酸 １６．０（重量％）
（２）ミリスチン酸 １６．０
（３）親油型モノステアリン酸グリセリン ２．０
（４）グリセリン ２０．０
（５）水酸化ナトリウム ７．５
（６）ヤシ油脂肪酸アミドプロピルベタイン １．０
（７）精製水 ３６．５
（８）シッポゴケ抽出物［製造例３］ １．０
製法：（１）〜（４）の油相成分を８０℃にて加熱溶解する。一方（５）〜（７）の水相成分を８０℃にて加熱溶解し、油相成分と均一に混合撹拌する。冷却を開始し、４０℃にて（８）を加え、均一に混合する。
[Prescription Example 7] Face-wash foam (1) Stearic acid 16.0 (% by weight)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 36.5
(8) Shippogoke extract [Production Example 3] 1.0
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.
［処方例８］メイクアップベースクリーム
（１）スクワラン １０．０(重量％)
（２）セタノール ２．０
（３）グリセリントリ−２−エチルヘキサン酸エステル ２．５
（４）親油型モノステアリン酸グリセリル １．０
（５）プロピレングリコール １１．０
（６）ショ糖脂肪酸エステル １．３
（７）精製水 ６９．４
（８）酸化チタン １．０
（９）ベンガラ ０．１
（10）黄酸化鉄 ０．４
（11）香料 ０．１
（12）シッポゴケ抽出物［製造例２］ １．２
製法：（１）〜（４）の油相成分を混合し、７５℃にて加熱溶解する。一方、（５）〜（７）の水相成分を混合し、７５℃にて加熱溶解し、これに（８）〜（10）の顔料を加え、ホモミキサーにて均一に分散させる。この水相成分に前記油相成分を加え、ホモミキサーにて乳化する。乳化終了後に冷却を開始し、４０℃にて（11）と（12）の成分を加え、均一に混合する。
[Prescription Example 8] Make-up base cream (1) Squalane 10.0 (% by weight)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 69.4
(8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Shippogoke extract [Production Example 2] 1.2
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.
［処方例９］乳液状ファンデーション
（１）メチルポリシロキサン ２．０(重量％)
（２）スクワラン ５．０
（３）ミリスチン酸オクチルドデシル ５．０
（４）セタノール １．０
（５）ポリオキシエチレン(２０Ｅ．Ｏ．)
ソルビタンモノステアリン酸エステル １．３
（６）モノステアリン酸ソルビタン ０．７
（７）１，３−ブチレングリコール ８．０
（８）キサンタンガム ０．１
（９）パラオキシ安息香酸メチル ０．１
（10）精製水 ５７．４
（11）酸化チタン ９．０
（12）タルク ７．４
（13）ベンガラ ０．５
（14）黄酸化鉄 １．１
（15）黒酸化鉄 ０．１
（16）香料 ０．１
（17）シッポゴケ抽出物［製造例４］ １．０
製法：（１）〜（６）の油相成分を混合し、７５℃にて加熱溶解する。一方、（７）〜（10）の水相成分を混合し、７５℃にて加熱溶解し、これに（11）〜（15）の顔料を加え、ホモミキサーにて均一に分散する。油相成分を加え、乳化を行う。乳化終了後に冷却を開始し、４０℃にて（16）と（17）の成分を順次加え、均一に混合する。
[Formulation Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (wt%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Purified water 57.4
(11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Shippogoke extract [Production Example 4] 1.0
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.
［処方例１０］油中水型エモリエントクリーム
（１）流動パラフィン ３０．０(重量％)
（２）マイクロクリスタリンワックス ２．０
（３）ワセリン ５．０
（４）ジグリセリンオレイン酸エステル ５．０
（５）塩化ナトリウム １．３
（６）塩化カリウム ０．１
（７）プロピレングリコール ３．０
（８）１，３−ブチレングリコール ５．０
（９）パラオキシ安息香酸メチル ０．１
（10）シッポゴケ抽出物［製造例１］ １．０
（11）精製水 ４７．４
（12）香料 ０．１
製法：（５）と（６）を（11）の一部に溶解して５０℃とし、５０℃に加熱した（４）に撹拌しながら徐々に加える。これを混合した後、７０℃にて加熱溶解した（１）〜（３）に均一に分散する。これに（７）〜（10）を（11）の残部に７０℃にて加熱溶解したものを撹拌しながら加え、ホモミキサーにて乳化する。乳化終了後に冷却を開始し、４０℃にて（12）を加え、均一に混合する。
[Formulation Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Shippogoke extract [Production Example 1] 1.0
(11) Purified water 47.4
(12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. while stirring and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.
［処方例１１］パック
（１）精製水 ５８．９(重量％)
（２）ポリビニルアルコール １２．０
（３）エタノール １７．０
（４）グリセリン ５．０
（５）ポリエチレングリコール（平均分子量１０００） ２．０
（６）シッポゴケ抽出物［製造例２］ ５．０
（７）香料 ０．１
製法：（２）と（３）を混合し、８０℃に加温した後、８０℃に加温した（１）に溶解する。均一に溶解した後、（４）と（５）を加え、攪拌しながら冷却を開始する。４０℃まで冷却し、（６）と（７）を加え、均一に混合する。
[Prescription Example 11] Pack (1) Purified water 58.9 (% by weight)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 5.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Shippogoke extract [Production Example 2] 5.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.
［処方例１２］入浴剤
（１）香料 ０．３(重量％)
（２）シッポゴケ抽出物［製造例１］ １．０
（３）炭酸水素ナトリウム ５０．０
（４）硫酸ナトリウム ４８．７
製法：（１）〜（４）を均一に混合する。
[Prescription Example 12] Bath agent (1) Fragrance 0.3 (% by weight)
(2) Shippogoke extract [Production Example 1] 1.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 48.7
Production method: (1) to (4) are mixed uniformly.
［処方例１３］ヘアーワックス
（１）ステアリン酸 ３．０(重量％)
（２）マイクロクリスタリンワックス ２．０
（３）セチルアルコール ３．０
（４）高重合メチルポリシロキサン ２．０
（５）メチルポリシロキサン ５．０
（６）ポリ（オキシエチレン・オキシプロピレン）
メチルポリシロキサン共重合体 １．０
（７）パラオキシ安息香酸メチル ０．１
（８）１，３−ブチレングリコール ７．５
（９）アルギニン ０．７
（10）精製水 ７３．６
（11）シッポゴケ抽出物［製造例１］ ２．０
（12）香料 ０．１
製法：（１）〜（６）の油相成分を混合し、７５℃にて加熱溶解後する。一方、（７）〜（10）の水相成分を７５℃にて加熱溶解し、前記油相成分を加え、ホモミキサーにて乳化する。乳化終了後に冷却を開始し、４０℃にて（11）と（12）の成分を加え、均一に混合する。
[Prescription Example 13] Hair wax (1) Stearic acid 3.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 73.6
(11) Shippogoke extract [Production Example 1] 2.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.
［処方例１４］ヘアートニック
（１）エタノール ５０．０(重量％)
（２）精製水 ４８．９
（３）シッポゴケ抽出物［製造例３］ １．０
（４）香料 ０．１
製法：（１）〜（４）の成分を混合，均一化する。
[Prescription Example 14] Hair artic (1) Ethanol 50.0 (% by weight)
(2) Purified water 48.9
(3) Shippogoke extract [Production Example 3] 1.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.
次に、シッポゴケ科植物抽出物を配合した処方を用いて使用試験を行い、シワ，タルミ，肌のハリ，シミ，クスミについて改善効果を評価した。その際、処方例１に示した乳液の処方に表５に記載するシッポゴケ科植物抽出物をそれぞれ配合し、実施例１〜５として使用試験を行った。また、シッポゴケ科植物抽出物を精製水に代替し、比較例１として同時に使用試験を行った。 Next, a use test was conducted using a prescription blended with an extract of the plant belonging to the plant family, and the improvement effect was evaluated for wrinkles, tarmi, skin firmness, spots, and kusumi. At that time, each of the extracts of the plant belonging to the plant family of Table 5 described in Table 5 was added to the formulation of the emulsion shown in Formulation Example 1, and the use test was conducted as Examples 1-5. In addition, as a comparative example 1, a use test was conducted at the same time, substituting the plant extract of Hippoceae to purified water.
各試料について、シワ，タルミ，肌のハリ，シミ，クスミといった症状が顕著に認められる４０〜６０才代の男女パネラー各２０名をそれぞれ一群とし、ブラインドにて２カ月間使用させ、使用前後の皮膚状態の変化を観察して評価した。皮膚症状の指標として、シワ，タルミ，肌のハリ，シミ，クスミについて、「改善」，「やや改善」，「変化なし」の三段階で評価し、表６に各評価を得たパネラー数にて示した。 For each sample, each group of 20 male and female panelists in their 40s to 60s who have noticeable symptoms such as wrinkles, tarmi, skin firmness, spots, and kusumi are used as a group for 2 months. Changes in skin condition were observed and evaluated. As indicators of skin symptoms, wrinkles, tarmi, skin firmness, stains, and kusumi were evaluated in three stages: “Improved”, “Slightly improved”, and “No change”. Showed.
表６より、シワ，タルミ，肌のハリ，シミ，クスミについて、シッポゴケ科植物抽出物を含有しない比較例使用群においては、６割以上のパネラーに改善は認められなかったが、シッポゴケ科植物抽出物を配合した実施例使用群においては、６割以上のパネラーに明確な改善が認められた。 From Table 6, for wrinkles, tarmi, skin firmness, stains, and kusumi, in the comparative example use group that does not contain the extract of the plant belonging to the plant family, more than 60% of panelists showed no improvement. In the example use group in which the product was blended, 60% or more of panelists clearly improved.
以上のように、本発明の実施例においては、従来の比較例よりも、シワ，タルミ，肌のハリ，シミ，クスミの改善に優れた効果を有していた。このことから、シッポゴケ科植物抽出物を配合した皮膚外用剤は、皮膚老化症状の防止・改善に優れた効果を発揮することが明らかとなった。
As described above, in the examples of the present invention, the effects of improving wrinkles, tarmi, skin firmness, spots, and stains were superior to those of the conventional comparative example. From this, it became clear that the skin external preparation which mix | blended the symptomaceae plant extract exhibits the effect excellent in prevention and improvement of a skin aging symptom.
Claims (8)
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| JP2001097992A (en) * | 1999-09-28 | 2001-04-10 | Snow Brand Milk Prod Co Ltd | New polyol-based compound |
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| JP2000273452A (en) * | 1999-03-24 | 2000-10-03 | Snow Brand Milk Prod Co Ltd | Antioxidant |
| JP2001097992A (en) * | 1999-09-28 | 2001-04-10 | Snow Brand Milk Prod Co Ltd | New polyol-based compound |
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