JP4515423B2 - Moisturizer, cell activator, and antioxidant - Google Patents

Description

  The present invention relates to a humectant, a cell activator, and an antioxidant. More specifically, the present invention relates to a moisturizer, a cell activator, and an antioxidant containing an extract of a genus Pomegranate plant, and a skin external preparation and food containing an extract of the genus Pomegranate.

  Causes of skin symptoms such as aging, ultraviolet rays, stress, wrinkles, stains, skin elasticity decrease, dryness, decreased cellular function, melanin production and pigmentation due to ultraviolet rays, decrease or degeneration of dermal matrix components, ultraviolet rays, etc. Examples include oxidative damage. In order to prevent and ameliorate such skin symptoms, search for various active ingredients and formulation studies have been conventionally conducted. As the cell activator, the essence of Ponkan (see Patent Document 1) is known, and as the antioxidant, an extract of the plant belonging to the genus Sarogase (see Patent Document 2) is known.

  In addition, the prior art regarding the moisturizer, cell activator, and antioxidant which use the extract of the genus Pomegranate as an active ingredient was not recognized.

JP 2001-131045 A Japanese Patent Laid-Open No. 10-182413

  Naturally-derived components are known to have various pharmacological and cosmetic effects, and so far many plants and fungi have been widely applied to fields such as external preparations for skin and foods and drinks. However, there are many naturally-derived components whose effects are not yet known, and the development of an effective component having an excellent moisturizing action, cell activation action or antioxidant action has been expected. The present invention has been made in order to find such an active ingredient, and provides a moisturizer, a cell activator, and an antioxidant that can be widely applied to fields such as external preparations for skin and foods and drinks. Objective.

  In order to find a moisturizer, a cell activator, and an antioxidant that can be widely applied to fields such as external preparations for skin and foods and drinks, the present inventors have examined various naturally-derived substances. As a result, the present inventors have found a moisturizing action, a cell activation action and an antioxidant action that are excellent in extracts of plants belonging to the genus Pomegranate, and have further studied to complete the present invention. That is, the present invention relates to a skin external preparation and food containing a moisturizer, a cell activator, an antioxidant, and an extract of one or more plants of the genus Pomegranate as an active ingredient. Is to provide.

  According to the present invention, it is possible to provide a moisturizer, a cell activator, and an antioxidant that have excellent effects. By blending these into a composition such as a skin external preparation or food, wrinkles, tarmi, It is possible to provide various compositions that exhibit an excellent effect in preventing and improving various skin symptoms such as skin firmness, spots, and kusumi.

As for the plant of the genus Pomegranate ( Mollugo ), which is a plant used as a raw material of the present invention, pomegranate ( Molego pentaphylla ), prunus prunus ( Molgo verticillata ) and the like are known.

  The plant as a raw material used in the present invention is not particularly limited as long as it is a plant belonging to the genus Pomegranate, but it is particularly preferable from the viewpoint of effectiveness to use pomegranate since it is relatively easy to obtain and effective. .

  The extract of the genus Pomegranate in the present invention includes the active substance and dried product of the genus Pomegranate, but it is preferable to use an extract extracted using various solvents. For extraction, any part of the leaves, flowers, seeds, roots, stems, buds, etc. of the genus Pomegranate may be used, but whole plants may be used for convenient use. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days.

  Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, ethers such as ethyl ether and propyl ether. And solvents such as esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  Extracts from the above-mentioned solvents of the genus Pomegranate can be used as they are, but the concentrated and dried solids can be used again by dissolving them in water or a polar solvent, and their physiological effects are not impaired. It may be used after performing purification treatment such as decolorization, deodorization and desalting, and fractionation treatment by column chromatography. The said extract of the genus Pomegranates, its processed material, and a fraction can also be used after freeze-drying after each processing and fractionation, and melt | dissolving at the time of use.

  The extract of the plant of the genus Pomegranate has excellent moisturizing action, cell activation action, and antioxidant action, and can be used as a moisturizing agent, cell activator, and antioxidant. In addition, the moisturizer, cell activator, and antioxidant containing the extract of the plant of the genus Pomegranate as an active ingredient can be used on the hair or taken orally, as well as applied to the skin. Or it can be applied to pharmaceuticals.

  A moisturizing agent containing an extract of the genus Pomegranate as an active ingredient exhibits an excellent moisturizing action on the skin and hair, and has a particularly high moisturizing effect on the skin.

  Although the cell activator which uses the extract of the genus Pomegranate plant as an active ingredient exhibits the outstanding activation effect | action with respect to various cells, especially exhibits the outstanding effect with respect to a dermal fibroblast.

  An antioxidant comprising an extract of the genus Pomegranate as an active ingredient exhibits an excellent antioxidant action, but particularly exhibits an excellent free radical scavenging action.

  In addition, by adding the extract of the plant of the genus Pomegranate to the external preparation for skin, it can be used externally for skin to prevent and improve skin symptoms such as wrinkles, tarmi, skin firmness, spots, kumi, dryness and fine lines. And can be used as a moisturizing skin external preparation, a whitening skin external preparation, or an anti-aging skin external preparation. Furthermore, the extract of the genus Pomegranate can also be used for foods intended for beauty, health maintenance or nutritional supplementation. In addition, a drink is also contained in the foodstuff of this invention.

  The amount of the extract of the genus Pomegranate belonging to the external preparation for skin can be adjusted according to the kind of external preparation for external use and the purpose of use. 0.0001 to 50.0% by weight is preferable, and 0.001 to 25.0% by weight is more preferable.

  The dosage form of the external preparation for skin containing the extract of the genus Pomegranate is arbitrary, and can be provided, for example, as a solubilizing system such as lotion, an emulsifying system such as cream or emulsion, or a dispersing system such as calamine lotion. Furthermore, it can also be provided in various dosage forms such as aerosols, ointments, powders and granules filled with a propellant.

  In addition, for the topical skin preparation containing the extract of the genus Pomegranate, in addition to the extract of the genus Pomegranate, in addition to the extract of the genus Pomegranate, if necessary, it is usually a pharmaceutical, a quasi-drug, skin cosmetic, hair cosmetic and washing Oil components, moisturizers, powders, pigments, emulsifiers, solubilizers, cleaning agents, UV absorbers, thickeners, drugs, fragrances, resins, antibacterial and antifungal agents, alcohols, etc. It can mix | blend suitably. Moreover, in the range which does not impair the effect of this invention, combined use with another moisturizer, a cell activator, or an antioxidant is also possible.

  In the following, production examples of extracts of the genus Pomegranates, tests for evaluating each action, examples of formulations for skin external preparations and foods, and use tests will be described in more detail, but the technical scope of the present invention is It is not limited at all.

[Production Example 1]
10 kg of a 50 wt% aqueous ethanol solution was added to 1 kg of a dry pulverized product of the genus Pomegranate and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, an extract of the plant of the genus Pomegranate was obtained.

[Production Example 2]
Nine liters of water was added to 1 kg of a dried pulverized plant of the genus Pomegranate and extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration, and after removing the solvent, an extract of the plant of the genus Pomegranate was obtained.

[Production Example 3]
Nine liters of methanol was added to 1 kg of a dry pulverized product of the genus Pomegranate and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, an extract of the plant of the genus Pomegranate was obtained.

[Production Example 4]
The plant of the genus Pomegranate was introduced into a supercritical extraction apparatus, and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under a pressure of 15 MPa. The extract was collected to obtain an extract of the plant of the genus Pomegranate.

  First, it shows about the cell activation effect of the extract of a genus Pomegranate. The sample was evaluated by extracting the whole plant of pomegranate according to Production Example 1 as a sample.

The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum. After culturing for 24 hours, the culture medium was replaced with a test medium to which a sample having an arbitrary concentration was added, and further cultured for 48 hours. Next, the medium containing 400 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was exchanged and cultured for 2 hours. Formazan generated by the opening of the tetrazolium ring was removed. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in Table 1 as relative values with the cell activation effect in the blank with no sample as 100. In the table, * and ** indicate a significance probability of less than 1% (P <0.05) and a significance probability of less than 1% (P <0.01), respectively, with respect to the significance P value in the t test. This is indicated by *.

  As is clear from Table 1, a significant dermal fibroblast activation effect was observed in the medium to which the extract of the genus Pomegranate was added. From this, it became clear that the extract of the plant of the genus Pomegranate has an excellent cell activation effect.

  Next, the antioxidant effect of the extract of the genus Pomegranate is shown. The sample was evaluated by extracting the whole plant of pomegranate according to Production Example 1 as a sample.

The evaluation was performed according to the following procedure. 100 μL of a sample diluted to an arbitrary concentration with a 50 wt% aqueous ethanol solution was added to a 96-well microplate. Next, 100 μL of a 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared with ethanol to a concentration of 0.2 mM was added to a 96-well microplate. The mixture was left in the dark with stirring, and the absorbance at 516 nm was measured after 24 hours. When the absorbance of the blank with no sample added was (A) and the absorbance when the sample was added was (B), the value of formula (1) was defined as the radical elimination rate. The evaluation results are shown in Table 2.
Formula (1) {1- (B) / (A)} × 100 (%)

  As is clear from Table 2, it was found that the extract of the plant of the genus Pomegranate has an antioxidant action based on radical scavenging.

  Then, the formulation example of the skin external preparation and the foodstuff which mix | blended the extract of the Pomegranates genus plant based on this invention is shown.

[Formulation Example 1] Emulsion (1) Squalane 10.0 (wt%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 57.85
(11) Arginine (1 wt% aqueous solution) 20.0
(12) Extract of Pomegranates plant [Production Example 1] 1.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix uniformly.

[Prescription Example 2] Lotion (1) Ethanol 15.0 (% by weight)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 82.38
(5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Extract of Pomegranates plant [Production Example 3] 1.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.

[Prescription Example 3] Cream (1) Squalane 10.0 (% by weight)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20% by weight aqueous solution) 15.0
(10) Purified water 40.7
(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Extract of Pomegranates plant [Production Example 1] 1.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification is completed, add (11), start cooling, add (12) at 40 ° C., and mix uniformly.

[Formulation Example 4] Cosmetic liquid (1) Purified water 27.45 (% by weight)
(2) Glycerol 14.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1 wt% aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by weight aqueous solution) 2.0
(16) Extract of Pomegranates [Production Example 1] 1.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C, add (16) and mix evenly.

[Formulation Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (% by weight)
(2) Purified water 86.7
(3) Sodium hydroxide (10% by weight aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Extract of Pomegranates plant [Production Example 4] 2.0
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Formulation Example 6] Cleansing Fee (1) Squalane 81.0 (wt%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Purified water 3.0
(4) Extract of Pomegranate plant [Production Example 4] 1.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.

[Prescription Example 7] Face-wash foam (1) Stearic acid 16.0 (% by weight)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 36.5
(8) Pomegranate plant extract [Production Example 3] 1.0
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.

[Prescription Example 8] Make-up base cream (1) Squalane 10.0 (% by weight)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 69.4
(8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Extract of Pomegranate plant [Production Example 2] 1.2
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Formulation Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (wt%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Purified water 57.4
(11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Extract of Pomegranates plant [Production Example 4] 1.0
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Formulation Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Extract of Pomegranates [Production Example 1] 1.0
(11) Purified water 47.4
(12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. while stirring, and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.

[Prescription Example 11] Pack (1) Purified water 62.9 (% by weight)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 5.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Extract of Pomegranate plant [Production Example 2] 1.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.

[Prescription Example 12] Bath agent (1) Fragrance 0.3 (% by weight)
(2) Extract of Pomegranates plant [Production Example 1] 1.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 48.7
Production method: (1) to (4) are mixed uniformly.

[Prescription Example 13] Hair wax (1) Stearic acid 3.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 74.6
(11) Extract of Pomegranates plant [Production Example 1] 1.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Prescription Example 14] Hair artic (1) Ethanol 50.0 (% by weight)
(2) Purified water 48.9
(3) Extract of Pomegranates plant [Production Example 3] 1.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

[Prescription Example 15] Tablet (1) Extract of plant of the genus Pomegranate [Production Example 1] 100.0 (mg)
(2) Reduced maltose starch syrup 461.0
(3) Corn starch 15.0
(4) Glycerin fatty acid ester 12.0
(5) Fragrance 12.0
Production method: (1) to (3) were respectively sieved and mixed, and then (4) to (5) were added and mixed. Thereafter, tableting was performed by a conventional method to obtain a tablet having a total amount of 600 mg.

  Next, a use test was conducted using a formulation containing an extract of the genus Pomegranate, and the effect of improving the rough skin due to drying was evaluated. At that time, the extract of the genus Pomegranate listed in Table 3 was added to the formulation of the emulsion shown in Formulation Example 1, and the use test was conducted as Examples 1-2. Moreover, the extract of the plant of the genus Pomegranate was replaced with purified water, and a use test was simultaneously conducted as Comparative Example 1.

  For each sample, 20 female panelists with dry skin in their 30s to 50s whose skin symptom was remarkably recognized were grouped and used for one week blindly, and the skin condition before and after use was observed and evaluated. . As an index of skin symptoms, rough skin due to dryness was evaluated in three stages of “improvement”, “slight improvement”, and “no change”, and Table 4 shows the number of panelists that obtained each evaluation.

  From Table 4, in the comparative example use group not containing the extract of the genus Pomegranate plant, improvement was not recognized in 70% or more of the panelers, but in the example use group containing the extract of the genus Pomegranate, More than 70% of panelists showed a clear improvement in rough skin. From this, it became clear that the extract of the plant of the genus Pomegranate has an excellent moisturizing effect.

Claims (1)

A moisturizing agent comprising as an active ingredient an extract of one or more plants selected from plants of the genus Pomegranate.