WO2008029879A1

WO2008029879A1 - Moisturizing agent, cell-activating agent and antioxidant

Info

Publication number: WO2008029879A1
Application number: PCT/JP2007/067405
Authority: WO
Inventors: Akira Hatani; Yumiko Okumura; Hayami Maeda
Original assignee: Noevir Co., Ltd.
Priority date: 2006-09-06
Filing date: 2007-09-06
Publication date: 2008-03-13
Also published as: JP2008063273A; JP4515423B2

Abstract

A moisturizing agent, a cell-activating agent and an antioxidant containing as an active ingredient one or more types of plants selected from the plants of the Molluginaceae genus or an extract thereof are disclosed. Further, various compositions containing a plant of the Molluginaceae genus or an extract thereof are disclosed.

Description

Specification

Moisturizers, cell activators, and antioxidants

Technical field

[0001] The present invention relates to a humectant, a cell activator, and an antioxidant containing a naturally derived ingredient as an active ingredient. More specifically, the present invention relates to the use of a plant of the genus Pomegranate or its extract. Background art

[0002] As factors of skin symptoms such as aging, ultraviolet rays, stress-induced skin wrinkles, spots, and skin elasticity decrease, dryness, cell function decline, melanin production and pigmentation due to ultraviolet rays, decrease and degeneration of dermal matrix components And oxidative damage of cells due to ultraviolet rays and the like. In order to prevent and improve such skin symptoms, various active ingredients have been searched and formulated. As the cell activator, the essence of Bonkan (Japanese Patent Laid-Open No. 2001-131 045) is known, and as the antioxidant, an extract of the plant belonging to the genus Sarogase (Japanese Patent Laid-Open No. 10-182413) is known.

Disclosure of the invention

[0003] Thus, various naturally derived components have been applied so far. However, there are many naturally-derived components whose effects are not yet known, and the development of active ingredients with excellent moisturizing action, cell activation action, or antioxidant action is expected. It was. The present invention develops natural ingredients having excellent moisturizing action, cell activation action, and antioxidant action, and provides a moisturizer, cell activator, antioxidant, and various compositions having these effects. With the goal.

[0004] As a result of examining various naturally derived components, the inventors of the present invention have excellent moisturizing action, cell activation action, and anti-antibacterial effect on pomegranate plants and their extracts, whose effects have not been known so far. It was found that an oxidizing action exists, and further studies were made to complete the present invention.

That is, according to the first aspect of the present invention, there are provided a humectant, a cell activator, and an antioxidant containing one or more plants selected from Pomegranate plants or an extract thereof as active ingredients. The According to a second aspect of the present invention, there is provided a composition containing one or more plants selected from the genus Pomegranate or two or more extracts thereof.

BEST MODE FOR CARRYING OUT THE INVENTION

[0005] The plant of the genus Pomegranate (Μ2ΐ1ΩΩ), which is a plant used as a raw material of the present invention, is an annual plant belonging to the family Pomegranate, and is widely distributed in Honshu, Shikoku and Kyushu. Pomegranate plant (Mollueo Oentaphvlla), Knoremanano (Mollugo verticillata), etc. The pomegranate plant or its extract contains so many kinds of ingredients that it cannot be analyzed. It is presumed that the various effects of the present invention can be obtained by acting comprehensively.

[0006] The plant used as a raw material is not particularly limited as long as it is a plant belonging to the genus Pomegranate, but it is particularly preferable to use pomegranate since it is relatively easy to obtain and effective. Multiple species of the genus Pomegranate can also be used in combination.

[0007] When using this plant of the genus Pomegranate, any part such as leaves, flowers, seeds, roots, stems, buds, etc., with no particular restrictions can be used. You can use multiple parts in combination. For easy use, use whole grass.

It is preferable to pulverize them as they are, and to use the raw material or the dried product, and use an extract from those parts.

[0008] For the extraction, any of the above-mentioned parts of the plant of the genus Pomegranate may be used, or an extract may be obtained using a plurality of parts. You can also use a mixture of two or more extracts extracted using different solvents.

In the extraction, the plant may be used as it is, but considering the extraction efficiency, it is preferable to carry out the extraction after processing such as shredding, drying, and grinding.

[0009] Extraction methods include room temperature, cooling or warming, extraction by immersing in an arbitrary extraction solvent for a predetermined time, extraction using a distillation method such as steam distillation, or from raw plants Examples of the pressing method include pressing to obtain an extract. Any of these methods can be used alone or in combination of two or more. Alternatively, extraction can be performed using a supercritical fluid or a subcritical fluid. Agitation to increase extraction efficiency Or may be homogenized in an extraction solvent. It is also possible to extract under a caloric pressure using an autoclave.

[0010] The extraction temperature is suitably about 5 ° C to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it for about 1 hour to 14 days.

The ratio of the plant and the solvent during the extraction is not particularly limited, but is preferably 0.5 to 5 times the solvent for the plant 1; Preferably 100 times by mass.

[0011] Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol and isopropanol; polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin; ethyl ether and propyl ether Solvents such as ethers such as butyl acetate, butyl acetate, ethyl acetate, and the like; ketones such as acetone, ethylmethylol ketone, and the like can be used. These may be used alone or in combination of any two or more. Saline, phosphate buffer, phosphate buffered saline and the like may be used. In addition, one or more supercritical liquids and subcritical liquids such as water, carbon dioxide, ethylene, propylene, ethanol, methanol, and ammonia may be used.

[0012] The extract of the above-mentioned plant of the genus Pomegranate can be used as it is, but it may be left to mature for a certain period of time, or may be used after being concentrated and dried to water or a polar solvent. It can also be used by dissolving. Alternatively, it may be used after performing purification treatment such as decolorization, deodorization, desalting, etc., or fractionation treatment by column chromatography or the like within a range not impairing these physiological functions. The above-mentioned extract of the genus Pomegranate, the treated product and fraction thereof can be freeze-dried after each treatment and fractionation, and dissolved in a solvent before use. It can also be used by encapsulating in vesicles such as ribosomes or microcapsules.

[0013] A plant of the genus Pomegranate or an extract thereof has excellent moisturizing action, cell activation action, and antioxidant action, and can be preferably used as a moisturizing agent, cell activator, and antioxidant. As long as each of these agents contains a pomegranate plant or an extract thereof as an active ingredient, its form and the presence or absence of other ingredients are not limited at all. As for the form, any form such as liquid, paste, gel, solid, powder, etc. can be selected according to its use etc., and the vehicle (excipient), solvent, Other general additives (antioxidants, colorants, dispersants, etc.) can optionally be included.

[0014] The amount of the pomegranate plant or extract thereof, which is an active ingredient in each agent, is adjusted with respect to the total amount from the viewpoint of power effect and stability that can be adjusted according to the type of agent and the purpose of use. , 0. 00001～ in terms of solid content; preferably from 100 mass ^_0/0 force Mashigu <is 0. 001-5 0 mass%.

[0015] A moisturizing agent comprising a pomegranate plant or an extract thereof as an active ingredient exerts an excellent moisturizing action on the skin and hair, and particularly has a high moisturizing effect on the skin.

[0016] A cell activator comprising a pomegranate plant or an extract thereof as an active ingredient exhibits an excellent activation action on various cells, and particularly exhibits an excellent effect on dermal fibroblasts.

[0017] An antioxidant comprising a pomegranate plant or an extract thereof as an active ingredient exhibits an excellent antioxidant action, but particularly exhibits an excellent free radical scavenging action.

[0018] Each of these agents can be used for hair and the like as well as applied externally to the skin, and can be applied to various compositions such as an external composition and an oral composition. Here, the composition for external use is not limited to any one category such as cosmetics, external preparations for skin, quasi-drugs, external medicines, etc., but any combination for external use on skin or hair. Means an adult. The term “oral composition” means any composition that can be taken orally, regardless of the type of drug, food, beverage, etc.

[0019] The dosage form of the composition for external use is arbitrary, and can be provided as, for example, a solubilizing system such as lotion, a dispersion system such as calamine mouth lotion, or an emulsifying system such as cream or emulsion. In addition, it can be provided in various dosage forms such as aerosol forms, ointments, and poultices filled with propellants.

Specifically, emulsions, creams, lotions, lotions, packs, serums, cleaning agents, lips Various types of cosmetics such as ticks, makeup cosmetics and foundations; various forms of quasi-drugs such as liquids, ointments, powders, granules, aerosols, patches, poultices, and external medicines can be exemplified.

[0020] The form of the oral composition is also arbitrary, and is in a liquid form such as a liquid, syrup, or extract, or a solid such as a granule, tablet, powder, capsule, or glaze, or a jelly, It can be processed and used in various forms such as gummi and gum, and is not particularly limited.

Specific examples include general foods including beverages, health foods (supplements), functional foods, nutritional supplements, oral medicines, and quasi drugs.

[0021] By adding a pomegranate plant or an extract thereof to an external preparation for skin including, for example, cosmetics, external medicines, quasi-drugs, etc. It is possible to obtain a composition for external use that exhibits an excellent effect on the prevention and improvement of various skin symptoms such as dullness, dullness, dryness, and fine wrinkles, and a skin external preparation for moisturizing, a skin external preparation for whitening, or an external skin for improving aging prevention. It can be used as an agent. In particular, it is preferably used as a skin external preparation for improving anti-aging containing pomegranate plant or an extract thereof.

Furthermore, pomegranate plant or its extract can also be used in foods and drinks, health foods (supplements), quasi-drugs, functional foods, etc. for the purpose of beauty, health maintenance or nutrition. Monkey.

[0022] In addition to pomegranate plants or extracts thereof, the composition for external use or oral use, etc., in addition to its use and necessity, usually skin cosmetics, hair cosmetics, pharmaceutical parts Optional components used in preparations such as external products and pharmaceuticals are included. Such components include water, oils (oil-based ingredients), alcohols, excipients, binders, extenders, disintegrants, corrigents, dyes, colorants, emulsifiers, solubilizers, dispersants, gelling agents. Agent, plasticizer, cleaning agent, UV absorber, thickener, pH adjuster, buffer agent, surfactant, lubricant, chelating agent, drug (medicinal component), fragrance, resin, coating agent Examples include fungicides, preservatives, preservatives, antioxidants, and pH adjusters. In addition, other moisturizers, cell activators, antioxidants, or plants other than the pomegranate plant or extracts thereof can be used as long as the effects of the present invention are not impaired. [0023] In the case of foods and drinks containing a plant of the genus Pomegranate or an extract thereof, the combination with various ingredients used in food is not particularly limited.

In other words, the dosage form of food is arbitrary, and can be provided in various dosage forms such as powders, granules, capsules, liquids, etc. Oil components, moisturizers, powders, emulsifiers, solubilizers, thickeners, drugs, fragrances, antifungal agents, alcohols, sugar, condensed milk, flour, salt, glucose, chicken eggs, butter, marga Phosphorus, chickenpox, calcium, iron, seasonings, spices, vitamin A and their derivatives, strength rotenoids, riboflavin and its derivatives, vitamin B and its salts or derivatives, ascorbic acid and its derivatives, cobalamins, vitamin E And their derivatives, vitamins, adenosine and its derivatives, flavonoids and tannins. Furthermore, it can be used in combination with other humectants, cell activators, or antioxidants as long as the effects of the present invention are not impaired.

[0024] The amount of the pomegranate plant or its extract in the composition for external use or oral composition can be adjusted according to the kind of composition, purpose of use, etc. in view of sex, based on the total amount, 0.1 in terms of solid content 00001-50. 0 wt% Ca, more preferably, 0.5 0001- 25. 0 mass ^_0/0. Furthermore, 0. 0001- 10 mass ^_0/0 Ca, more preferably 0.000;! A ~ 5 mass ^_0/0, more preferably 0.001 to 5 wt%, more preferably 0. 0;! To 5% by mass, particularly preferably 0 .;! To 5% by mass.

Example

[0025] Hereinafter, production examples of extracts of the plant of the genus Pomegranates, tests for evaluating each action, formulation examples as external preparations for skin and foods, and use tests will be described in more detail, but the technical scope of the present invention Is not limited by these.

10 liters of 50% by weight ethanol aqueous solution was added to 1 kg of dry crushed pomegranate plant and immersed for 7 days at room temperature. The extract was collected by filtration, and the solvent was removed to obtain extract 1 of whole pomegranate plant.

<Extract production example 2> 9 liters of water was added to 1 kg of dry ground pulverized pomegranate plant, and the mixture was extracted by refluxing at 90 ° C for 6 hours. The extract was collected by filtration, and the solvent was removed to obtain an extract 2 of whole pomegranate plant.

Nine liters of methanol was added to 1 kg of dry ground pulverized pomegranate plant and soaked at room temperature for 7 days. The extract was collected by filtration, and the solvent was removed to obtain an extract 3 of whole pomegranate plant.

[0029] <Extract production example 4>

The whole pomegranate plant was put into a supercritical extractor and extracted using a supercritical fluid of carbon dioxide at 40 ° C under 15 MPa pressure. The extract was recovered to obtain extract 4 of whole pomegranate plant.

<0030> <Evaluation of human dermal fibroblast activation effect>

Using the extract 1, the dermal fibroblast activation effect was evaluated as follows.

Normal human dermal fibroblasts manufactured by Kurashiki Boseki Co., Ltd. were seeded in a 96-well microphone mouth plate so that 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% by weight urchin fetal serum (FBS). After culturing for 24 hours, the sample culture medium was adjusted to each sample concentration shown in Table 1 with 1 mass ° / 83-added DMEM medium, and further cultured for 48 hours. After removing the supernatant, replace the medium with 400 µg / ml of 3- (4,5-dimethyl-2-thioazolyl) -2,5-diphenyltetrazolium bromide (MTT reagent). Cultured for 2 hours. Thereafter, the formazan produced by the opening of the tetrazolium ring was extracted with 2-propanol, and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values.

The obtained results are shown in Table 1 as relative values when the cell activation effect in the control without addition of the sample is taken as 100. * And ** in the table indicate a significance probability of less than 1% (P <0. 05) for * and a significance probability of less than 1% (P <0. 01) for the significance value P in the t-test. * Represents each one.

[0031] [Table 1] Additive strength

Cell activation

(mg / mL)

Blank 1 00

0. 031 1 05 *

0. 063 1 08 *

0. 1 25 1 09 *

0. 250 1 1 3 **

[0032] As is clear from Table 1, in the medium supplemented with the extract of the plant of the genus Pomegranate, a significant dermal fibroblast activation effect was observed. From this, it was revealed that the extract of the plant of the genus Pomegranate has an excellent cell activation effect.

[0033] <Evaluation of antioxidant effect by DPPH radical scavenging>

Using the above Extract 1, the antioxidant effect by DPPH radical scavenging was evaluated as follows.

Using 50% by mass ethanol, the sample solution was adjusted so that each sample concentration shown in Table 2 was obtained, and 100 / j l was added to a 96-well microphone opening plate. Thereto, 0.2 mM of 1,1-diphenyl-2-picrylhydrazyl (DPPH) ethanol solution was added in increments of 1001 and mixed well, and allowed to stand at room temperature for 24 hours. Finally, the absorbance at 516 nm derived from DPPH radical was measured.

The extinction rate of the DPPH radical was calculated from the following equation, where (A) is the absorbance when no sample is added and (B) is the absorbance when the sample is added.

Radical scavenging rate = {1— (B) / (A)} X 100

The results are shown in Table 2.

[0034] [Table 2]

[0035] As clearly shown in Table 2, it was found that the extract of the plant of the genus Pomegranate had an antioxidant action based on radical scavenging.

[0036] Next, formulation examples for carrying out the present invention will be shown. [Prescription Example 1] Emulsion

[Table A]

(1) Scorran 10. 0 (mass%)

(2) Methylphenylpolysiloxane 4.0

(3) Hydrogenated palm kernel oil 0.5

(4) Hydrogenated soybean phospholipid 0.1

(5) Polyoxyethylene monostearate

Sonorbitan (20 E. O.) 1. 3

(6) Sorbitan monostearate1.0

(7) Glycerin 4.0

(8) Methyl paraoxybenzoate 0.1

(9) Carboxyvinyl polymer 0.15

(10) Purified water 57. 85

(11) Arginine (1 mass% aqueous solution) 20. 0

(12) Extract of Pomegranate plant 1 1.0 Production method: Heat-dissolve oil phase components (1) to (6) at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix evenly.

[Prescription Example 2] Lotion

[Table B]

(1) Ethanol 15.0 (mass%)

(2) Polyoxyethylene (40E. O.) Hardened castor oil 0.3

(3) Fragrance 0. 1

(4) Purified water 82. 38

(5) Chenic acid 0.02

(6) sodium taenoate 0.1

(7) Glycerin 1.0

(8) Hydrochestino Resenorelose 0.1

(9) Extract of Zaku mouth sow plant 3 1.0 Production method: Dissolve ( ² ) and (3) in (1). After dissolution, add (4) to (8) sequentially, and then stir well. Add (9) and mix uniformly.

[Prescription Example 3] Cream

[Table C] (1) Skull Run 1 0. 0

(2) Stearic acid 2.0

(3) Hydrogenated palm kernel oil 0.5

(4) Hydrogenated soybean phospholipid 0.1

(5) Setano Inore 3.6

(6) Lipophilic glyceryl monostearate2.0

(7) Glycerin 1 0. 0

(8) Methyl paraoxybenzoate 0.1

(9) Arginine (20 mass ⁰ /. Aqueous solution) 1 5. 0

(10) Purified water 40.7

(11) Carboxyvinyl polymer (1% by weight aqueous solution) 1 5. 0

(12) Extract of Zaku mouth sow plant 1 1. 0 Production method: Heat-dissolve the oil phase components of (1) to (6) at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, add (11), start cooling, add (12) at 40 ° C and mix uniformly.

[Prescription Example 4] Essence

[Table D]

(1) Purified water 27.4

(2) Glycerin 14.0

(3) Sucrose fatty acid ester 1.3

(4) Carboxyvinyl polymer (1% by weight aqueous solution) 1 7.5

(5) Sodium alginate (1% by weight aqueous solution) 1 5.0

(6) Polyglyceryl monolaurate 1.0

(7) Macadamia nut oil fatty acid phytosteryl 3.0

(8) N-lauroyl-L-dalamic acid

Di (one phytosteryl 2-octyldodecyl) 2.0

(9) Hardened palm oil 2.0

(10) Sukuran (Olive) 1.0

(11) Behenyl alcohol 0.7

(12) Beeslow 1. 0

(13) Hohono oil 1. 0

(14) 1, 3-butylene glycol 1 0. 0

(15) L_Arginine (10 mass /. Aqueous solution) 2.0

(16) Extract of Zaku mouth sow plant 1 1.0 Production method: Mix the water phase components of (1) to (6) and dissolve by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Start cooling after emulsification and add (15) at 50 ° C. Cool to 40 ° C and add (16) to mix evenly. Match.

[Prescription Example 5] Aqueous jewel

[Table E]

(1) Carboxyvinyl polymer 0.5

(2) Purified water 86.7

(3) Sodium hydroxide (10 mass% aqueous solution) 0.5

(4) Ethanol 1 0. 0

(5) Methyl paraoxybenzoate 0.1

(6) Fragrance 0. 1

(7) Pomegranate plant extract 4 2. 0

(8) Polyoxyethylene (60 E. O.) hydrogenated cinnamon oil 0.1 Production method: Add (1) to (2), stir uniformly, and then add (3). After stirring uniformly, add (5) previously dissolved in (4). After stirring uniformly, add (6) to (8) previously mixed and stir and mix uniformly.

[Prescription Example 6] Cleansing Fee

[Table F]

(1) Sukuwaran 8 1.0 (mass ^_0/0)

(2) Polyoxyethylene glyceryl isostearate 1 5. 0

(3) Purified water 3.0

(4) Extract of Pomegranate plant 4 1.0 Production method: Dissolve (1) and (2) uniformly. Add (3) and (4) to this, and mix evenly.

[Prescription Example 7] Face-wash form

[Table G]

(1) Stearic acid 1 6.0

(2) Myristic acid 1 6. 0

(3) Lipophilic glyceryl monostearate2.0

(4) Glycerin 20.0

(5) Sodium hydroxide 7.5

(6) Coconut oil fatty acid aminopropyl propyne 1.0

(7) Purified water 36.5

(8) Extract of Pomegranate plant 3 1.0 Manufacturing method: Heat-dissolve oil phase components (1) to (4) at 80 ° C. On the other hand, the water phase components (5) to (7) are heated and dissolved at 80 ° C, and the oil phase components are mixed and stirred uniformly. Start cooling, add (8) at 40 ° C, and mix uniformly. [Prescription Example 8] Makeup Base Tari

[Table H]

(1) School run 10. 0 ()

(2) Setano Inore 2.0

(3) Glycerol tri-2-ethylhexanoate 2.5

(4) Lipophilic glyceryl monostearate1.0

(5) Propylene glycolate 1 1. 0

(6) Sucrose fatty acid ester 1.3

(7) Purified water 69.4

(8) Titanium oxide 1.0

(9) Bengala 0.1

(10) Yellow iron oxide 0.4

(11) Fragrance 0. 1

(12) Extract of Zaku mouth sow plant 2 1.2 Production method: Mix the oil phase components of (1) to (4) and dissolve by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed, dissolved by heating at 75 ° C, and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Start cooling after emulsification, add ingredients (11) and (12) at 40 ° C and mix evenly.

[Prescription Example 9] Milky Foundation

[Table I]

(1) Methyl polysiloxane 2.0 (mass ^_0/0)

(2) Skullang 5.0

(3) Octyldodecyl myristate 5.0

(4) Setano Nore 1. 0

(5) Polyoxyethylene (20 E. O.)

Sonorebitan monostearate Estenole 1.3

(6) Sorbitan monostearate 0.7

(7) 1,3-Butylene glycol 8.0

(8) Xanthan gum 0.1

(9) Methyl paraoxybenzoate 0.1

(10) Purified water 57.4

(11) Titanium oxide 9.0

(12) Talc 7.4

(13) Bengala 0.5

(14) Yellow iron oxide 1.1

(15) Black iron oxide 0.1

(16) Fragrance 0. 1

(17) Extract of Zaku mouth sow plant 4 1.0 Production method: Mix the oil phase components of (1) to ½) and dissolve by heating at 75 ° C. On the other hand, (7) to (10) The aqueous phase components are mixed, dissolved by heating at 75 ° C, and the pigments (11) to (15) are added thereto and dispersed uniformly with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after emulsification, and components (16) and (17) are added sequentially at 40 ° C and mixed uniformly.

[Prescription Example 10] Water-in-oil emollient cream

[Table J]

(1) Liquid paraffin 30.0 ([[

(2) Microcrystalline wax 2.0

(3) Vaseline 5.0

(4) Diglycerinoleate 5.0

(5) Sodium chloride 1.3

(6) Potassium chloride 0.1

(7) Propylene glycolate 3.0

(8) 1, 3-butylene glycol 5.0

(9) Methyl paraoxybenzoate 0.1

(10) Extract of Pomegranate plant 1 1. 0

(11) Purified water 47.4

(12) Fragrance 0.1 Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C, heat to 50 ° C and gradually add to (4) while stirring. After mixing this, disperse uniformly into (1) to (3), which is heated and dissolved at 70 ° C. Add (7) to (10), which is dissolved in the remainder of (11) by heating at 70 ° C while stirring, and emulsify with a homomixer. Start cooling after emulsification, add (12) at 40 ° C, and mix evenly.

[Prescription Example 11] Pack

[Table K]

(1) Purified water 62.9 (mass%)

(2) Polyvinylenoreconole 12.0

(3) Ethanol 17.0

(4) Glycerin 5.0

(5) Polyethylene glycol (average molecular weight 1000) 2.0

(6) Zaku Mouth Extract 2 1.0

(7) Fragrance 0.1 Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After evenly dissolving, add (4) and (5) and start cooling with stirring. Cool to 40 ° C, add (6) and (7), and mix evenly.

[Prescription Example 12] Bath salt [Representation]

(1) Perfume 0.3 (mass ^_0/0)

(2) Pomegranate plant extract 0

(3) Sodium bicarbonate 50. 0

(4) Sodium sulfate 48.7 Production method: Mix (1) to (4) uniformly.

[Prescription Example 13] Hair wax

[Table M]

(1) Stearic acid 3.0 ('

(2) Microcrystalline wax 2.0

(3) Cetino Reanolo-Nole 3.0

(4) Highly polymerized methylpolysiloxane 2.0

(5) Methylpolysiloxane 5.0

(6) Poly (oxyethylene.oxypropylene)

Methylpolysiloxane copolymer 1.0

(7) Methyl paraoxybenzoate 0.1

(8) 1,3-Butylene glycol 7.5

(9) Arginine 0.7

(10) Purified water 74.6

(11) Extract of Pomegranate plant 1 1. 0

(12) Fragrance 0.1 Production method: Mix the oil phase components (1) to (6), and heat and dissolve at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C, and the oil phase component is added and emulsified with a homomixer. Start cooling after emulsification, add ingredients (11) and (12) at 40 ° C and mix evenly

.

[Prescription Example 14] Hair Nick

Tsuyoshi

(1) Ethanol 50.0 (mass%)

(2) Purified water 48.9

(3) Pomegranate plant extract 3 1. 0

(4) Fragrance 0.1 Production method: Mix and homogenize components (1) to (4).

[Prescription Example 15] Tablet

[Table Ο] (1) Pomegranate plant extract 1 100. 0 (mg)

(2) Reduced maltose starch syrup 461.0

(3) Corn starch 15. 0

(4) Glycerin fatty acid ester 12.0

(5) Fragrance 12.0 Production method: (1) to (3) were respectively sieved and mixed, then (4) to (5) were added and mixed. Thereafter, tableting was performed by a conventional method to obtain a tablet having a total amount of 600 mg.

[0052] Next, a use test was conducted using a formulation containing an extract of the genus Pomegranate plant, and the effect of improving the rough skin due to drying was evaluated. At that time, in the emulsion formulation shown in Formulation Example 1, each of the extracts listed in Table 3 was blended as component (12), and a use test was conducted as Examples; As Comparative Example 1, an emulsion in which the extract of component (12) was replaced with purified water was used, and a use test was simultaneously conducted.

[0053] [Table 3]

[0054] For each sample, a group of 20 women with dry skin in their 30s to 50s who had markedly rough skin symptoms, each group was used blindly for 1 week to change the skin condition before and after use. Observed and evaluated. As an index of skin symptoms, rough skin due to dryness was evaluated in three stages: “Improved”, “Slightly improved”, and “No change”.

Table 4 shows the number of panelists that obtained each evaluation.

[0055] [Table 4]

[0056] From Table 4, in the comparative use group that does not contain the extract of the genus Pomegranate plant, improvement was not recognized by more than 70% of the panelists. In the case of more than 70% of the panelists, clear improvement of rough skin was observed. From this, it is clear that pomegranate plant extracts have an excellent moisturizing effect It became.

[0057] According to the present invention, a moisturizer, a cell activator, and an antioxidant having excellent effects can be provided by using a pomegranate plant or an extract thereof as an active ingredient. Furthermore, by blending pomegranate plant or its extract into skin preparations (or compositions for external use) such as cosmetics, external medicines, or oral compositions such as foods, wrinkles, tarmi, skin firmness or skin It is possible to provide various compositions that exhibit an excellent effect in preventing and improving various dermatological conditions such as low elasticity of the skin, spots, dullness, dryness, and fine wrinkles.

[0058] The disclosure of the present application is related to the subject matter described in Japanese Patent Application No. 2006-242157 filed on September 6, 2006, the entire contents of which are incorporated herein by reference.

It should be noted that various modifications and changes may be made to the above-described embodiments without departing from the novel and advantageous features of the present invention other than those already described. Accordingly, all such modifications and changes are intended to be included within the scope of the appended claims.

Claims

The scope of the claims

[1] A moisturizing agent containing one or more kinds of plants selected from the genus Pomegranate or an extract thereof as an effective component.

[2] A cell activator comprising, as an active ingredient, one or more plants selected from Pomegranate plants or an extract thereof.

[3] An antioxidant comprising one or more plants selected from Pomegranate plants or an extract thereof as an active ingredient.

[4] A composition for external use containing one or more plants selected from the genus Pomegranate, or an extract thereof.

[5] An oral composition comprising one or two or more plants selected from Pomegranate plants or an extract thereof.