JP2011225477A - Anti-aging agent, antioxidant, external preparation for skin, and functional oral composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an anti-aging agent and an antioxidant, which can be applied widely to fields of an external preparation for skin, a functional oral composition and the like.SOLUTION: The anti-aging agent, the antioxidant, the external preparation for skin and the functional oral composition each includes an extract of floral buds of Sasa kurilensis of genus Sasa, Poaceae family.

Description

  The present invention relates to an anti-aging agent, an antioxidant, an external preparation for skin, and a functional oral composition containing a flower bud of Gramineae Sasamasa or an extract thereof.

Causes of skin symptoms such as aging, disease, stress, wrinkles due to ultraviolet rays, blemishes, reduced skin elasticity, dryness, decreased cellular function, melanin production and pigmentation due to ultraviolet rays, decrease or degeneration of dermal matrix components, ultraviolet rays, etc. Examples include cell oxidative damage. In order to prevent and ameliorate such skin symptoms, various active ingredients have been searched and formulated. In particular, naturally-derived components are known to have various pharmacological and cosmetic effects, and so far, applications of extracts such as plants and fungi to skin external preparations and oral compositions have been studied.

  For example, in order to obtain a skin external preparation having an anti-aging and improving action on the skin, an ingredient derived from Kintoki grass (see Patent Document 1) as an ingredient having an activating action of dermal fibroblasts, an active oxygen scavenging action and an excessive effect. Sweet pea extract (see Patent Document 2) and the like are already known as components having a radical scavenging action in order to obtain an antioxidant having a lipid oxide production inhibitory action. Moreover, about the component obtained from Chishimazasa, it was publicly known that the distillate obtained by fractionating the juice obtained by compressing raw leaves and stems has antibacterial and moisturizing effects. However, the component obtained from the flower bud of Chishimazasa has not been known so far.

JP 2008-174459 A JP 2008-285637 A JP 2004-224750 A

  Thus, various naturally-derived components have been applied so far. However, there are many naturally-derived components whose effects are not yet known, and the development of effective components having excellent anti-aging and antioxidant effects has been expected.

  As a result of examining various components derived from the natural environment, the present inventors have found that the anti-aging action and the anti-oxidation action are excellent in the flower buds of the genus Sasa genus Sasamasa which were not known in the past or their extracts. The present invention was completed through further investigations.

  That is, the present invention relates to an anti-aging agent, an antioxidant, an external preparation for skin, and a functional oral composition containing the flower buds of the genus Saceae or the extract thereof.

  According to the present invention, it is possible to provide an anti-aging agent, an antioxidant, an external preparation for skin, and a functional oral composition having an excellent effect by blending a flower bud of Gramineae Sasa genus or an extract thereof. it can.

  The Sasa kurilensis (Ruprecht) Makino et Shibata used in the present invention is a plant belonging to the genus Sasa genus, Soma kurenensis (Ruprecht). It is. The main areas of distribution are the Sea of Japan side of Honshu, north of Tottori, the Tohoku region, Hokkaido, and other areas. The flowers are spiked and are said to bloom only once every tens to hundreds of years (sixty years in one theory), but when they bloom, the whole community blooms and dies after fruiting.

  In the extraction, the plant may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization.

  The extraction can be performed by immersing in an arbitrary extraction solvent for a predetermined time. The extraction solvent may be heated as necessary. Alternatively, extraction can be performed using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be stirred or homogenized in an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but is suitably about 1 hour to 14 days.

  As an extraction solvent, in addition to water, lower alcohols such as methanol, ethanol, propanol and isopropanol; polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin; ethers such as ethyl ether and propyl ether Solvents such as esters; esters such as butyl acetate and ethyl acetate; ketones such as acetone and ethyl methyl ketone can be used. These may be used alone or in combination of any two or more. Saline, phosphate buffer, phosphate buffered saline, and the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical liquids and subcritical liquids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  The extract of the flower bud of Chishimasa can be used as it is, but it can be used as it is by standing for a certain period of time, and it can be used by aging, or the concentrated and dried product can be dissolved again in water or a polar solvent. Can also be used. Alternatively, it may be used after performing purification treatment such as decolorization, deodorization, and desalting, and fractionation treatment by column chromatography or the like within a range not impairing these physiological functions. The extract of the flower buds of Chishimasa, its processed product and fractions thereof can be freeze-dried after each treatment and fractionation and dissolved in a solvent before use. It can also be used by encapsulating in vesicles such as liposomes or microcapsules.

  The flower bud of Chishimasa or its extract has an excellent anti-aging effect and antioxidant effect, and can be used as an anti-aging agent, an antioxidant, an external preparation for skin, and a functional oral composition.

  An anti-aging agent containing the flower bud of Chishimasa or its extract as an active ingredient has an excellent human dermal fibroblast activation effect and human epidermal keratinocyte activation effect, and exhibits an excellent effect in preventing and improving aging symptoms To do.

  Antioxidants containing the flower buds or extracts thereof as an active ingredient have an excellent DPPH radical scavenging action and exhibit an excellent antioxidant effect.

  An external preparation for skin containing flower buds of Chichimasasa or an extract thereof exhibits excellent anti-aging and antioxidant effects.

  The functional oral composition containing the flower bud of Chishimasa or its extract exhibits an excellent anti-aging effect and antioxidant effect.

  Each of these agents is not limited at all in terms of its form and the presence or absence of other components, as long as it contains the flower buds or extracts thereof as active ingredients. As for the form, any form such as liquid, paste, gel, solid, powder, etc. can be selected according to its use and the like, vehicle (excipient), solvent, Other general additives (antioxidants, colorants, dispersants, etc.) can optionally be included.

  Here, the external preparation for skin means all external compositions for external use on the skin or hair, such as cosmetics, quasi-drugs, and external medicines. The functional oral composition also means all compositions that are taken orally regardless of the type of pharmaceuticals, foods, beverages and the like.

  The dosage form of the external preparation for skin is arbitrary, and can be provided, for example, as a solubilizing system such as lotion, a dispersion system such as calamine lotion, or an emulsifying system such as cream or emulsion. Further, it can be provided in various dosage forms such as an aerosol form, an ointment, and a poultice filled with a propellant.

  Specifically, various cosmetics such as emulsions, creams, lotions, lotions, packs, cosmetic liquids, cleaning agents, makeup cosmetics, etc .; liquids, ointments, powders, granules, aerosols, patches, poultices, etc. Various forms of cosmetics, quasi drugs, topical medicines, and the like can be exemplified.

  The form of the functional oral composition is also arbitrary and is not particularly limited. Specifically, general foods including beverages; health foods (supplements) such as tablets, capsules, granules, powders or functional foods; oral drugs such as tablets, capsules, granules, powders, syrups, extracts Etc. can be exemplified.

  For topical skin preparations or functional oral compositions, in addition to flower buds or extracts thereof, pharmaceuticals, quasi-drugs, skin cosmetics, hair cosmetics and cleansing agents, etc. Optional ingredients usually formulated in water, such as water, oily ingredients, moisturizers, powders, pigments, emulsifiers, solubilizers, gelling agents, cleaning agents, UV absorbers, anti-inflammatory agents, thickeners, pH adjustment Agents, chelating agents, drugs (medicinal ingredients), fragrances, resins, antibacterial and antifungal agents, antioxidants, alcohols and the like can be appropriately blended. Furthermore, other moisturizers, anti-aging agents, whitening agents, antioxidants and slimming agents, or plants other than Tishimasa or an extract thereof can be used as long as the effects of the present invention are not impaired.

  Also in the case of oral compositions such as foods and drinks, there is no particular limitation in combination with various components that are usually used for oral use.

  The amount of flower buds or their extracts in the topical skin preparation or functional oral composition can be adjusted depending on the type and purpose, but from the standpoint of efficacy and stability, In terms of minutes, 0.0001 to 10.0% by mass is preferable, more preferably 0.001 to 5.0% by mass, still more preferably 0.01 to 5% by mass, and still more preferably 0.00. 1 to 5% by mass.

  In the following, preparation examples of flower bud extract of Chishimasa, test method for evaluating anti-aging effect / antioxidant effect, skin preparation for external use, formulation example as functional food will be described in more detail. The range is not limited by this.

[Extract 1: Ethanol extract]
Chishimasa (part: flower bud) was dried and pulverized, 50 vol% ethanol of 20 times the sample mass was added and extracted at room temperature with stirring for 3 hours, and then insolubles were removed by filtration. After concentration under reduced pressure, freeze-drying was performed to obtain an extract.

[Extract 2: Hot water extract]
Chishimasa (part: flower bud) was dried and pulverized, purified water 20 times the mass of the sample was added, and the mixture was extracted by heating at 120 ° C. for 20 minutes in an autoclave. An insoluble material was removed by suction filtration while maintaining a high temperature, and then lyophilized to obtain an extract.

  Using the above extract, the anti-aging effect and the antioxidant effect were evaluated for the flower buds of Chishimasa. In addition, ** described in each evaluation result represents a significance probability of less than 1% (P <0.01) by ** with respect to the significance probability P value in the t test.

<Anti-aging effect (human dermal fibroblast activation)>
Evaluation of the human dermal fibroblast activation effect of the flower bud extract of Chishimasa was performed by the method shown below. Extract 1 was used as a sample.

Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS) was used as the seeding medium. After culturing for 24 hours, the culture medium was replaced with 0.5% by mass FBS-added DMEM medium to which the extract 1 was added so as to have each concentration shown in Table 1, and further cultured for 24 hours. Next, the MTT reagent adjusted with the culture medium so that it might be set to 100 microgram / mL was added to the cell except the supernatant, and it culture | cultivated for about 2 hours. Finally, formazan produced in 2-propanol was extracted, and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated using the difference between the two measured values. The evaluation results are shown in Table 1 as relative values with the cell activation effect in the control with no sample added being taken as 100.

  As is apparent from Table 1, the flower bud extract of Chishimazasa exhibits a significant human dermal fibroblast activation effect, and thus exhibits an excellent anti-aging effect.

<Anti-aging effect (human epidermal keratinocyte activation action)>
The human epidermis keratinocyte activation effect of the flower bud extract of Chishimasa was evaluated by the method shown below. Extract 1 was used as a sample.

Normal human epidermal keratinocytes NHEK were seeded in a 96-well microplate so as to be 2.0 × 10 ⁴ cells per well. Humedia-KG2 medium (Kurabo NHEK growth medium) was used as the seeding medium. After culturing for 24 hours, the culture medium was replaced with KG2 medium supplemented with extract 1 so that the concentrations shown in Table 2 were obtained, and further cultured for 24 hours. Next, the MTT reagent adjusted with the culture medium so that it might be set to 100 microgram / mL was added to the cell except the supernatant, and it culture | cultivated for about 2 hours. Finally, formazan produced in 2-propanol was extracted, and the absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated using the difference between the two measured values. The evaluation results are shown in Table 2 as relative values when the cell activation effect in the control with no sample added is taken as 100.

  As is clear from Table 2, the flower bud extract of Chishimazasa exhibits a significant anti-aging effect due to its significant human epidermal keratinocyte activation effect.

  As shown in Tables 1 and 2, the flower bud extract of Chishimazasa exhibits an excellent anti-aging effect since it exhibits a human dermal fibroblast activation action and a human epidermal keratinocyte activation action.

<Antioxidant effect (DPPH radical scavenging action)>
Evaluation of DPPH radical scavenging action of the flower bud extract of Chishimasa was performed by the following method. Extract 1 was used as a sample.

Add 100 μL of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) ethanol solution to 100 μL of the sample solution prepared by extracting Extract 1 with each concentration shown in Table 2 with 50 mass% ethanol. After mixing well, the mixture was allowed to stand in the dark at room temperature for 24 hours, and the absorbance at 516 nm derived from the DPPH radical was measured. When the absorbance when no sample is added is (A) and the absorbance when the sample is added is (B), the DPPH radical elimination rate is defined by the following equation.
Erase rate = {1- (B) / (A)} × 100
The evaluation results are shown in Table 3.

  As apparent from Table 3, the flower bud extract of Chishimazasa exhibits a high antioxidant effect due to its high DPPH radical scavenging action.

  Then, the formulation example of the skin external preparation and functional oral composition which mix | blended the flower bud extract of the tishima masa obtained by each said preparation method is shown.

[Example 1] Emulsion (1) Squalane 10.0 (mass%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 100 (11) Arginine (1% by weight aqueous solution) 20.0
(12) Extract 1 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After cooling, (11) and (12) are sequentially added at 40 ° C. and mixed uniformly.

[Example 2] Lotion (1) Ethanol 15.0 (mass%)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 100 (5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Extract 2 1.0
Production method: (2) and (3) are dissolved in (1). Further, after sequentially adding (4) to (8), the mixture is sufficiently stirred, and (9) is added and mixed uniformly.

[Example 3] Cream (1) Squalane 10.0 (mass%)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20 mass% aqueous solution) 15.0
(10) Remainder 100 (11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Extract 1 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. (11) is added and stirred, then cooled and (12) is added at 40 ° C. and mixed uniformly.

[Example 4] Cosmetic liquid (1) The balance (mass%) of purified water 100
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1% by weight aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (derived from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by mass aqueous solution) 2.0
(16) Extract 2 3.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. After cooling, add (15) at 50 ° C. and (16) at 40 ° C. and mix uniformly.

[Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (mass%)
(2) The balance made into purified water 100 (3) Sodium hydroxide (10 mass% aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Extract 2 0.5
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 1.0
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Example 6] Cleansing fee (1) Squalane 81.0 (mass%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Extract 1 4.0
Manufacturing method: (1) and (2) are uniformly dissolved. Add (3) to this and mix uniformly.

[Example 7] Face-wash foam (1) Stearic acid 16.0 (mass%)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 25.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) The balance which is made into purified water 100 (8) Extract 2 0.1
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the water phase components (5) to (7) are heated and dissolved at 80 ° C., and the oil phase components are uniformly mixed and stirred. After cooling, add (8) at 40 ° C. and mix uniformly.

[Example 8] Make-up base cream (1) Squalane 10.2 (% by mass)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) The balance which makes 100 purified water (8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Extract 1 3.0
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. After cooling, the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (mass%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Remainder water 100 (11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Extract 2 0.5
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the water phase components (7) to (10) are mixed, dissolved by heating at 75 ° C., the pigments (11) to (15) are added thereto, and the mixture is uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. After cooling, components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Example 10] Water-in-oil emollient cream (1) Liquid paraffin 34.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Extract 1 3.0
(11) The balance of 100 purified water (12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C, and gradually add to (4) heated to 50 ° C with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. with stirring, and emulsified with a homomixer. After cooling, add (12) at 40 ° C. and mix uniformly.

[Example 11] The balance (mass%) of pack (1) purified water 100
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 9.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Extract 2 1.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After evenly dissolving, add (4) and (5) and cool with stirring. Add (6) and (7) at 40 ° C. and mix uniformly.

[Example 12] Bath agent (1) Fragrance 0.3 (mass%)
(2) Extract 1 3.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 46.7
Production method: (1) to (4) are mixed uniformly.

[Example 13] Hair wax (1) Stearic acid 3.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 100 (11) Extract 2 2.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. After cooling, the components (11) and (12) are added at 40 ° C. and mixed uniformly.

Example 14 Hair artic (1) Ethanol 50.0 (mass%)
(2) The balance of purified water 100 (3) Extract 1 3.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

Example 15 Tablet (1) Corn Starch 44.0 (mass%)
(2) Crystalline cellulose 100 (3) Carboxymethylcellulose calcium 5.0
(4) Silicic anhydride 0.5
(5) Magnesium stearate 0.5
(6) Extract 1 5.0
Production method: (1) to (6) are uniformly mixed, and compression-molded with a tableting machine to obtain one tablet of 200 mg.

[Example 16] Powder (1) Magnesium aluminate silicate 95.3 (% by mass)
(2) Carboxymethylcellulose calcium 100 The remainder (3) Extract 1 4.0
Production method: After the powders (1) to (3) are mixed, they are pulverized by a pulverizer and uniformly dispersed.

[Example 17] Candy (1) Sucrose 60.0 (mass%)
(2) Remaining as Minamata 100 (3) Extract 1 5.0
(4) Fragrance Appropriate amount Manufacturing method: (1) and (2) are heated, mixed and homogenized, cooled, components (3) and (4) are added at 70 ° C., mixed and homogenized, and then molded.

[Example 18] Drink (1) Aminoethylsulfonic acid 1000 mg
(2) Thiamine nitrate 10mg
(3) Riboflavin sodium phosphate 5mg
(4) Pyridoxine hydrochloride 10mg
(5) Anhydrous caffeine 50mg
(6) Citric acid 250mg
(7) D-sorbitol solution 8mg
(8) Extract 1 1000 mg
(9) Fragrance Trace amount (10) Purified water 100 mL of the remaining manufacturing method: (1) to (9) are sequentially added to (10) and homogenized.

  The skin external preparations shown in Examples 1 to 14 were compositions having an anti-aging action and an anti-oxidation action. The functional oral compositions shown in Examples 15 to 18 were compositions having an anti-aging action and an antioxidant action.

Claims (4)

  An anti-aging agent comprising, as an active ingredient, an extract of flower buds of the genus Sasa kurilensis.   An antioxidant comprising an extract of flower buds of Sasa kurilensis as an active ingredient.   An external preparation for skin containing, as an active ingredient, an extract of flower buds of Sasa kurilensis.   A functional oral composition comprising, as an active ingredient, an extract of flower buds of the genus Sasa kurilensis.