JP4291647B2 - Cell activator, whitening agent, collagen production promoter, antioxidant, and skin external preparation - Google Patents

Description

The present invention relates to a cell activator, a whitening agent, a collagen production promoter, an antioxidant, and a skin external preparation. More specifically, the present invention relates to a cell activator, a whitening agent, a collagen production promoter, an antioxidant, and an external preparation for skin containing an extract of one or two or more kinds of plants selected from the plant of the family Bartramiaceae .

  Causes of aging symptoms such as wrinkles, blemishes, and skin elasticity decline due to aging and ultraviolet rays, etc. due to cell function decline, melanin production and pigmentation due to ultraviolet rays, decrease and degeneration of dermal matrix components such as hyaluronic acid and collagen, ultraviolet rays, etc. Examples include oxidative damage of cells. In order to prevent and ameliorate such aging symptoms, search for various active ingredients and formulation studies have been conventionally conducted. As a cell activator, essence of Ponkan (see Patent Document 1), as a whitening agent, water and / or organic solvent extract of Hakutsuru Reishi (see Patent Document 2), and as a collagen production promoter, retinoid and beech An extract from a tree bud of a family Beech (see Patent Document 3) and an extract of a Salugaceae plant (see Patent Document 4) are known as antioxidants.

  In addition, the prior art regarding a cell activator, a whitening agent, a collagen production promoter, an antioxidant, and an external preparation for skin, which contains an extract of an Agrobacterium plant as an active ingredient, was not recognized.

JP 2001-131045 A JP 2003-89630 A Japanese Patent Laid-Open No. 2001-278783 Japanese Patent Laid-Open No. 10-182413

  Conventionally used cell activators, whitening agents, collagen production promoters and antioxidants may be insufficient as essential effects, and the development of better active ingredients has been expected. The present invention has been made in view of such conventional problems, and is an active ingredient that is naturally derived and highly safe, and has an excellent cell activation action, whitening action, collagen production promoting action, and antioxidant action. It is intended to provide a skin external preparation useful for finding and improving aging.

  In order to solve the above-described problems, the present inventors have studied various naturally-derived substances with respect to actions useful for preventing and improving aging. As a result, they found excellent cell activation, whitening, collagen production promoting, and antioxidant effects in extracts of one or more plants selected from the department of the family Asteraceae, and completed further investigations to complete the present invention. It came to do. That is, the present invention provides a cell activator, a whitening agent, a collagen production promoter, an antioxidant, and an external preparation for skin, each of which contains an extract of one or two or more plant species of the family Arophoraceae. It is.

  ADVANTAGE OF THE INVENTION According to this invention, the cell activator, whitening agent, collagen production promoter, and antioxidant which have the outstanding effect can be provided. In addition, by adding these to skin external preparations, skin external preparations and foods for improving aging prevention that exhibit excellent effects in the prevention and improvement of skin aging symptoms such as wrinkles, tarmi, skin firmness, spots, and scum The composition can be provided.

The plant used as a raw material of the present invention may be a plant of the family Bartramiaceae . The Tamagoke plant belonging to the family, Tamagoke genus (Bartramia), Uwabamigoke genus (Breutelia), Nagakubisawagoke genus, Sawagoke genus (Philonotis), such as Ezotamagoke genus are known. Examples of the plant of the genus Tamagoke (Bartramia), Kumomatamagoke (Bartramia halleriana), Stewartia Tamagoke (Bartramia ithyphylla), such as Ootamagoke (Bartramia pomiformis) is known. Known plants of the genus Breutelia are Breutelia arundinifolia and the like. Examples of the plant of the genus Nagakubisawagoke (Fleischerobryum), such as Nagakubisawagoke (Fleischerobryum longicolle) is known. The plant Sawagoke genus (Philonotis), Kamasawagoke (Philonotis falcata), Orehasawagoke (Philonotis falcata var. Carinata), Sawagoke (Philonotis fontana), Soroibasawagoke (Philonotis fontana var. Seriata), Horai Sawa moss (Philonotis hastata), Nagabasawagoke ( Philonotis lancifolia), Makibasawagoke (Philonotis nitida), Princess Makiba Sawa moss (Philonotis secunda), Kotsukushisawagoke (Philonotis thwaitesii), Oosawagoke (Philonotis turneri na), such as Ezosawagoke (Philonotis yezoana) is known. Examples of the plant of the genus Ezotamagoke (Plagiopus), such as Ezotamagoke (Plagiopus oederiana) is known.

The plant used as a raw material used in the present invention is not particularly limited as long as it is an Asteraceae plant, but for reasons such as availability and effectiveness, the genus Bartramia, the plant of the genus Breutelia ( Brutelia ) Can be suitably used. Tamagoke genus (Bartramia) plants, the Uwabamigoke genus (Breutelia) plants, Ootamagoke (Bartramia pomiformis), it is preferable to use a Uwabamigoke (Breutelia arundinifolia), particularly preferred from the viewpoint that the effectiveness of using Ootamagoke.

  When using these Asteraceae plants, it is common to use an extract. For extraction, any part of the sporophyte and gametophyte of the family Chrysomelidae may be used, but for easy use, the whole plant of the spore and gametophyte may be used. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days.

  Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, and ethers such as ethyl ether and propyl ether. , Solvents such as esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these are selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  Extracts of the above-mentioned Tamagoceae plants with the above-mentioned solvents can be used as they are, but they are decolorized and deodorized as long as the concentrated and dried solids are dissolved again in water or a polar solvent, or the physiological functions thereof are not impaired. It may be used after a purification treatment such as desalting or a fractionation treatment by column chromatography or the like. The above-mentioned extract of Leguminosae plant and its treated product and fractionated product can be freeze-dried after each treatment and fractionated and dissolved in a solvent at the time of use.

  An extract of the plant belonging to the family Asteraceae has an excellent cell activation effect, whitening effect, collagen production promoting effect, and antioxidant effect, and can be used as a cell activator, whitening agent, collagen production promoting agent, and antioxidant. .

  A cell activator comprising an extract of the department of Trichaceae as an active ingredient exerts an excellent activation action on various cells, but particularly exhibits an excellent effect on dermal fibroblasts.

  A whitening agent containing an extract of an Agrobacterium plant as an active ingredient is effective in improving pigmentation symptoms such as spots and freckles, and is particularly effective in suppressing melanin production based on inhibition of tyrosinase activity.

  Collagen production promoters containing an extract of the department of Trichaceae as an active ingredient exhibit an excellent effect in promoting the production of dermal matrix components, and in particular, an excellent effect in promoting the production of collagen.

  Antioxidants containing an extract of the family Clamaceae as an active ingredient exhibit an excellent antioxidant action, and particularly an excellent free radical scavenging action.

  Moreover, the skin external preparation for anti-aging improvement which exhibits the effect excellent in prevention and improvement of a skin aging symptom can be obtained by mix | blending the extract of the department of an Asteraceae plant with a skin external preparation. Furthermore, the extract of the family Arophoraceae can also be used in foods and beverages for the purpose of maintaining health and providing nutrition.

  The blending amount of the extract of the genus Tamagoceae in the skin external preparation can be adjusted depending on the type of skin external preparation and the purpose of use, etc., but from the point of effect and stability, 0.0001-50.0 weight% is preferable, More preferably, it is 0.001-20.0 weight%.

  The dosage form of the external preparation for skin containing the extract of the genus Tamagoceae is arbitrary, and can be provided as a solubilizing system such as lotion, an emulsifying system such as cream or emulsion, or a dispersing system such as calamine lotion. Furthermore, it can also be provided in various dosage forms such as aerosols, ointments, powders and granules filled with a propellant.

  In addition, for the topical skin preparation containing the extract of the department of the department of the department, in addition to the extract of the department of the department of the family Rosaceae, if necessary, it is usually a pharmaceutical, a quasi-drug, a skin cosmetic, a cosmetic for hair and a washing. Oil components, moisturizers, powders, pigments, emulsifiers, solubilizers, cleaning agents, UV absorbers, thickeners, drugs, fragrances, resins, antibacterial and antifungal agents, alcohols, etc. It can mix | blend suitably. Moreover, in the range which does not impair the effect of this invention, combined use with another cell activator, a whitening agent, a collagen production promoter, and an antioxidant is also possible.

  In the following, production examples of the extract of the plant belonging to the family Asteraceae, tests for evaluating each action, formulation examples as an external preparation for skin, and use tests will be described in more detail, but the technical scope of the present invention is not limited thereby. It is not limited. First, an example of the production of an extract of the plant belonging to the family Asteraceae is shown.

[Production Example 1]
10 liters of a 50 wt% aqueous ethanol solution was added to 1 kg of a dry pulverized whole plant of the Asteraceae and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, an extract of the family Asteraceae was obtained.

[Production Example 2]
Nine liters of water was added to 1 kg of the dried ground pulverized plant of Tamagoceae and the mixture was extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration, and after removing the solvent, an extract of the family Asteraceae was obtained.

[Production Example 3]
Nine liters of methanol was added to 1 kg of a dried pulverized whole plant of the Asteraceae plant, and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, an extract of the family Asteraceae was obtained.

[Production Example 4]
The whole plant of the Asteraceae plant was put into a supercritical extraction apparatus and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under a pressure of 15 MPa. The extract was collected to obtain an extract from the family Leguminosae.

  Next, it shows about the activation effect | action of the dermis fibroblast of an Amaranthaceae plant extract. The sample used was a pokeweed extract extracted from the giant pokeweed using Production Example 1.

The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum. After culturing for 24 hours, the culture medium was replaced with a test medium to which a sample having an arbitrary concentration was added, and further cultured for 48 hours. Next, the medium containing 400 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was exchanged and cultured for 2 hours. Formazan produced by the opening of the tetrazolium ring was removed. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in Table 1 as relative values with the cell activation effect in the blank with no sample as 100. In addition, ** in the table represents a significance probability of less than 1% (P <0.01) with respect to the significance probability P value in the t test.

  As is clear from Table 1, a significant dermal fibroblast activation effect was observed in the medium supplemented with the pokeweed extract. In particular, when 0.125 to 0.5 mg / mL of the pokeweed extract was added, a significant dermal fibroblast activation effect was observed at a risk rate of less than 1% compared to the blank. From this, it was clarified that the pokeweed extract has an excellent dermal fibroblast activation effect.

  Next, the evaluation of the melanin production inhibitory action of the extract of the plant belonging to the family Arociaceae is shown. The sample used was a pokeweed extract extracted from the giant pokeweed using Production Example 1.

  The evaluation was performed according to the following procedure. B16 mouse melanoma F0 strain (B16F0) cells were seeded in a 35 mm dish at 2000 cells per dish. After culturing for 24 hours, the culture medium was replaced with Dulbecco's modified Eagle medium (DMEM) supplemented with 5 wt% fetal calf serum (FCS) to which a sample of an arbitrary concentration was added. After culturing for 7 days, the cells were collected using 0.25% trypsin, transferred to a 1.5 mL microtube, and centrifuged to obtain a cell precipitate. Furthermore, instead of the medium to which the sample was added, a Dulbecco's modified Eagle medium (DMEM) to which 5 wt% fetal calf serum (FCS) was added was used as a blank, and visual determination was similarly performed. Further, as shown in Table 2, the visual judgment was performed by a five-step evaluation. Table 3 shows the determination results.

  As is clear from Table 3, a significant melanin production inhibitory effect was observed when a medium supplemented with the pokeweed extract was used. In particular, almost no blackening was observed when 0.125 mg / mL of the pokeweed extract was added. From this, it has been clarified that the pokeweed extract has a high whitening action based on the melanin production inhibitory action.

  Next, the evaluation of the collagen production promoting action of the plant department plant extract is shown. The sample used was a pokeweed extract extracted from the giant pokeweed using Production Example 1.

The evaluation was performed according to the following procedure. Dulbecco's modified basal medium supplemented with 1.0 wt% fetal bovine serum supplemented with an arbitrary concentration of a sample, seeded in a 96-well microplate with normal human dermal fibroblasts at 2.0 × 10 ⁴ per well. DMEM) was cultured at 37 ° C. for 24 hours, and the amount of collagen in the culture supernatant was measured by ELISA. At the same time, the number of fibroblasts was counted, the amount of collagen production per cell was calculated, and the relative amount of collagen production per blank cell containing no sample as 100 was shown in Table 4. In addition, ** in the table represents a significance probability less than 1% (P <0.01) with respect to the significance probability P value in the t test.

  As is clear from Table 5, a significant collagen production-promoting effect was observed in the medium to which the pokeweed extract was added, compared to the case where it was not added. In particular, when the pokeweed extract was added in an amount of 0.063 to 0.125 mg / mL, a significant collagen production promoting effect was observed with a risk rate of less than 1%. From this, it was clarified that the pokeweed extract has an excellent collagen production promoting action.

  Next, it shows about the antioxidant effect of the Amaranthaceae plant extract. The sample used was a pokeweed extract extracted from the giant pokeweed using Production Example 1.

The evaluation was performed according to the following procedure. 100 μL of a sample solution diluted to an arbitrary concentration with a 50 wt% aqueous ethanol solution was added to a 96-well microplate. Next, 100 μL of a 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared with ethanol to a concentration of 0.2 mM was added to a 96-well microplate. The mixture was left in the dark with stirring, and the absorbance at 516 nm was measured after 24 hours. When the absorbance of the blank with no sample added was (A) and the absorbance when the sample was added was (B), the value of formula (1) was defined as the radical elimination rate. The evaluation results are shown in Table 5.
Formula (1) {1- (B) / (A)} × 100 (%)

  As can be seen from Table 5, the pokeweed extract has an excellent antioxidant effect.

  Then, the prescription example of the skin external preparation which mix | blended the Agaricaceae plant extract which concerns on this invention is shown.

[Formulation Example 1] Emulsion (1) Squalane 10.0 (wt%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 53.85
(11) Arginine (1 wt% aqueous solution) 20.0
(12) Otamatamaki Extract [Production Example 1] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix uniformly.

[Prescription Example 2] Lotion (1) Ethanol 15.0 (% by weight)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 78.38
(5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Otamatamaki extract [Production Example 3] 5.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.

[Prescription Example 3] Cream (1) Squalane 10.0 (% by weight)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20% by weight aqueous solution) 15.0
(10) Purified water 40.7
(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Otamatamaki Extract [Production Example 1] 1.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification is completed, add (11), start cooling, add (12) at 40 ° C., and mix uniformly.

[Formulation Example 4] Cosmetic liquid (1) Purified water 27.45 (% by weight)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1 wt% aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by weight aqueous solution) 2.0
(16) Otamatama extract [Production Example 1] 5.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C, add (16) and mix evenly.

[Formulation Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (% by weight)
(2) Purified water 86.7
(3) Sodium hydroxide (10% by weight aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Otamatama extract [Production Example 4] 2.0
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, add (5) previously dissolved in (4). After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Formulation Example 6] Cleansing Fee (1) Squalane 81.0 (wt%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Purified water 3.0
(4) Otamatamaki extract [Production Example 4] 1.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.

[Prescription Example 7] Face-wash foam (1) Stearic acid 16.0 (% by weight)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 36.5
(8) Otamatamaki extract [Production Example 3] 1.0
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.

[Prescription Example 8] Make-up base cream (1) Squalane 10.0 (% by weight)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 69.4
(8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Otamatamaki extract [Production Example 2] 1.2
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Formulation Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (wt%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Purified water 57.4
(11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Otamatamaki extract [Production Example 4] 1.0
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Formulation Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Agaricum extract [Production Example 1] 1.0
(11) Purified water 47.4
(12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. while stirring and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.

[Prescription Example 11] Pack (1) Purified water 58.9 (% by weight)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 5.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Otamatamaki extract [Production Example 2] 5.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.

[Prescription Example 12] Bath agent (1) Fragrance 0.3 (% by weight)
(2) Otamatamaki extract [Production Example 1] 1.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 48.7
Production method: (1) to (4) are mixed uniformly.

[Prescription Example 13] Hair wax (1) Stearic acid 3.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 73.6
(11) Otamatama extract [Production Example 1] 2.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Prescription Example 14] Hair artic (1) Ethanol 50.0 (% by weight)
(2) Purified water 48.9
(3) Otamatamaki extract [Production Example 3] 1.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

  Next, a use test was conducted using a prescription containing an extract of the plant belonging to the department of Amaranthaceae, and the improvement effect was evaluated for wrinkles, tarmi, skin firmness, spots, and kuzumi. At that time, each of the eggplant family plant extracts listed in Table 6 was blended with the emulsion formulation shown in Formulation Example 1, and a use test was conducted as Examples 1-6. In addition, a use test was conducted at the same time as Comparative Example 1 by substituting the purified plant water with the extract of the plant belonging to the family Asteraceae.

  For each sample, each group of 20 male and female panelists in their 40s to 60s who have noticeable symptoms such as wrinkles, tarmi, skin firmness, spots, and kusumi are used as a group for 2 months. Changes in skin condition were observed and evaluated. As indicators of skin symptoms, wrinkles, tarmi, skin firmness, spots, and kusumi were evaluated in three stages: “Improved”, “Slightly improved”, and “No change”. Showed.

  From Table 7, for wrinkles, tarmi, skin firmness, stains, and kusumi, in the comparative use group that does not contain the extract of the plant family, no improvement was observed in more than 70% of the panelists, In the example use group in which the product was blended, 60% or more of panelists clearly improved.

As described above, in the examples of the present invention, the effects of improving wrinkles, tarmi, skin firmness, spots, and stains were superior to those of the conventional comparative example. From this, it became clear that the skin external preparation which mix | blended the Aromaceae plant extract exhibits the effect excellent in prevention and improvement of a skin aging symptom.

Claims (4)

  A cell activator characterized by containing a pokeweed extract.   Whitening agent characterized by containing Otamatamake extract.   Collagen production promoter characterized by containing Otamamagoke extract.   A skin external preparation for improving aging prevention, characterized by containing an Otamatamake extract.