JP4480366B2 - Cell activator, whitening agent, hyaluronic acid production promoter, collagen production promoter, antioxidant, and skin external preparation - Google Patents
Cell activator, whitening agent, hyaluronic acid production promoter, collagen production promoter, antioxidant, and skin external preparation Download PDFInfo
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- JP4480366B2 JP4480366B2 JP2003295754A JP2003295754A JP4480366B2 JP 4480366 B2 JP4480366 B2 JP 4480366B2 JP 2003295754 A JP2003295754 A JP 2003295754A JP 2003295754 A JP2003295754 A JP 2003295754A JP 4480366 B2 JP4480366 B2 JP 4480366B2
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- extract
- production promoter
- hyaluronic acid
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- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 239000003380 propellant Substances 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 150000004492 retinoid derivatives Chemical class 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 235000020637 scallop Nutrition 0.000 description 1
- 230000037394 skin elasticity Effects 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 238000000194 supercritical-fluid extraction Methods 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 125000003831 tetrazolyl group Chemical group 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
- CPYIZQLXMGRKSW-UHFFFAOYSA-N zinc;iron(3+);oxygen(2-) Chemical compound [O-2].[O-2].[O-2].[O-2].[Fe+3].[Fe+3].[Zn+2] CPYIZQLXMGRKSW-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Description
本発明は、細胞賦活剤、美白剤、ヒアルロン酸産生促進剤、コラーゲン産生促進剤、抗酸化剤、及び皮膚外用剤に関する。さらに詳しくは、シラガゴケ科(Leucobryaceae)植物より選ばれる1種又は2種以上の植物の抽出物を含有する細胞賦活剤、美白剤、ヒアルロン酸産生促進剤、コラーゲン産生促進剤、抗酸化剤、及び皮膚外用剤に関する。 The present invention relates to a cell activator, a whitening agent, a hyaluronic acid production promoter, a collagen production promoter, an antioxidant, and a skin external preparation. More particularly, Shiragagoke family (Leucobryaceae) 1, two or more cell activator containing extract of a plant selected from plants, whitening agents, hyaluronic acid production-promoting agent, the collagen production promoter, an antioxidant, and It relates to an external preparation for skin.
加齢や紫外線などによるシワ,シミ,皮膚の弾性低下といった老化症状の要因として、細胞機能低下、紫外線によるメラニン産生や色素沈着、ヒアルロン酸やコラーゲン等の真皮マトリックス成分の減少や変性、紫外線等による細胞の酸化傷害などが挙げられる。このような老化症状を防止・改善するために、様々な有効成分の検索及び配合検討が従来なされてきた。細胞賦活剤としては、ポンカンのエッセンス(特許文献1参照)、美白剤としては、白鶴霊芝の水および/または有機溶媒抽出物(特許文献2参照)、ヒアルロン酸産生促進剤としては、アナアオサの抽出物(特許文献3参照)、コラーゲン産生促進剤としては、レチノイドとブナ科ブナ属植物の木の芽からの抽出物(特許文献4参照)、抗酸化剤としては、サルオガセ科サルオガセ属植物の抽出物(特許文献5参照)が知られている。 Causes of aging symptoms such as wrinkles, blemishes, and skin elasticity decline due to aging and ultraviolet rays, etc. due to cell function decline, melanin production and pigmentation due to ultraviolet rays, decrease and degeneration of dermal matrix components such as hyaluronic acid and collagen, ultraviolet rays, etc. Examples include oxidative damage of cells. In order to prevent and ameliorate such aging symptoms, search for various active ingredients and formulation studies have been conventionally conducted. As a cell activator, the essence of Ponkan (see Patent Document 1), as a whitening agent, water and / or organic solvent extract of Hakutsuru Reishi (see Patent Document 2), and as a hyaluronic acid production promoter, Anaaosa's An extract (see Patent Document 3), an extract from a retinoid and a bud of a beech family beech (see Patent Document 4) as a collagen production promoter, and an extract of a plant from the genus Sarogase family as an antioxidant (See Patent Document 5).
なお、シラガゴケ科植物の抽出物を有効成分とする細胞賦活剤、美白剤、ヒアルロン酸産生促進剤、コラーゲン産生促進剤、抗酸化剤、及び皮膚外用剤に関する先行技術は認められなかった。 In addition, the prior art regarding a cell activator, a whitening agent, a hyaluronic acid production promoter, a collagen production promoter, an antioxidant, and a skin external preparation containing an extract of the plant belonging to the family Shiragagoceae as an active ingredient was not recognized.
従来用いられている細胞賦活剤、美白剤、ヒアルロン酸産生促進剤、コラーゲン産生促進剤、抗酸化剤は、本質的な効果としては不十分な場合もあり、より優れた有効成分の開発が期待されていた。本発明は、このような従来の問題点に鑑みてなされたものであり、天然由来で安全性が高く、優れた細胞賦活作用、美白作用、ヒアルロン酸産生促進作用、コラーゲン産生促進作用、抗酸化作用を有する有効成分を見出し、老化防止・改善に有用な皮膚外用剤を提供することを目的とする。 Conventionally used cell activators, whitening agents, hyaluronic acid production promoters, collagen production promoters and antioxidants may be insufficient as essential effects, and the development of better active ingredients is expected. It had been. The present invention has been made in view of such conventional problems, is naturally derived and highly safe, has an excellent cell activation effect, whitening effect, hyaluronic acid production promoting effect, collagen production promoting effect, antioxidant An object of the present invention is to find an active ingredient having an action and to provide an external preparation for skin useful for preventing and improving aging.
本発明者らは、上記の課題を解決するために、老化防止・改善に有用な作用に関して、天然由来の種々の物質について検討を行った。その結果、シラガゴケ科植物より選ばれる1種又は2種以上の植物の抽出物に優れた細胞賦活作用、美白作用、ヒアルロン酸産生促進作用、コラーゲン産生促進作用、抗酸化作用を見出し、さらに検討を重ね、本発明を完成するに至った。すなわち、本発明は、シラガゴケ科植物の1種または2種以上の植物の抽出物を有効成分とする細胞賦活剤、美白剤、ヒアルロン酸産生促進剤、コラーゲン産生促進剤、抗酸化剤、及び皮膚外用剤を提供するものである。 In order to solve the above-described problems, the present inventors have studied various naturally-derived substances with respect to actions useful for preventing and improving aging. As a result, we have found excellent cell activation, whitening, hyaluronic acid production, collagen production, and antioxidant activity in one or more plant extracts selected from the plant of the moss family. Repeatedly, the present invention has been completed. That is, the present invention relates to a cell activator, a whitening agent, a hyaluronic acid production promoter, a collagen production promoter, an antioxidant, and a skin, each of which contains an extract of one or more plant species of the moss family. An external preparation is provided.
本発明によれば、優れた効果を有する細胞賦活剤、美白剤、ヒアルロン酸産生促進剤、コラーゲン産生促進剤、抗酸化剤を提供することができる。また、これらを皮膚外用剤に配合することにより、シワ,タルミ,肌のハリ,シミ,クスミといった皮膚老化症状の防止・改善に優れた効果を発揮する老化防止改善用の皮膚外用剤や食品等の組成物を提供することができる。 ADVANTAGE OF THE INVENTION According to this invention, the cell activator, whitening agent, hyaluronic acid production promoter, collagen production promoter, and antioxidant which have the outstanding effect can be provided. In addition, by adding these to skin external preparations, skin external preparations and foods for improving aging prevention that exhibit excellent effects in the prevention and improvement of skin aging symptoms such as wrinkles, tarmi, skin firmness, spots, and scum The composition can be provided.
本発明の原料として用いられる植物は、シラガゴケ科(Leucobryaceae)の植物であればよい。シラガゴケ科植物としては、シラガゴケ属(Leucobryum),ニセハブタエゴケ属(Leucophanes)などが知られている。シラガゴケ属(Leucobryum)の植物としては、アラハシラガゴケ(Leucobryum bowringii),シロシラガゴケ(Leucobryum glaucum),ジャバシラガゴケ(Leucobryum javense),ホソバオキナゴケ(Leucobryum juniperoideum),オオシラガゴケ(Leucobryum scabrum)などが知られている。ニセハブタエゴケ属(Leucophanes)の植物としては、ニセハブタエゴケ(Leucophanes octoblepharioides)などが知られている。 The plant used as a raw material of the present invention may be a plant of the family Leucobraceaceae . Examples of the plant belonging to the family Shiragagoceae include the genus Leucobryum and the genus Leucophanes . The plant Shiragagoke genus (Leucobryum), Arahashiragagoke (Leucobryum bowringii), white Shiragagoke (Leucobryum glaucum), Java Shiragagoke (Leucobryum javense), Hosobaokinagoke (Leucobryum juniperoideum), etc. Ooshiragagoke (Leucobryum scabrum) is known. Examples of the plant of the genus Nisehabutaegoke (Leucophanes), such as Nisehabutaegoke (Leucophanes octoblepharioides) is known.
本発明に用いられる原料となる植物は、シラガゴケ科植物であれば特に限定されないが、入手が比較的容易なことや有効性などの理由から、シラガゴケ属(Leucobryum)植物,ニセハブタエゴケ属(Leucophanes)を好適に用いることが出来る。シラガゴケ属(Leucobryum)植物,ニセハブタエゴケ属(Leucophanes)としては、ホソバオキナゴケ(Leucobryum juniperoideum),オオシラガゴケ(Leucobryum scabrum),アラハシラガゴケ(Leucobryum bowringii),ニセハブタエゴケ(Leucophanes octoblepharioides)を用いることが好ましく、ホソバオキナゴケ,オオシラガゴケ,アラハシラガゴケを用いることが有効性の点から特に好ましい。 Plants as a raw material used in the present invention is not particularly limited as long as it is Shiragagoke family plant, because such availability is relatively easy for and effectiveness, Shiragagoke genus (Leucobryum) plants Nisehabutaegoke genus (Leucophanes) It can be suitably used. Shiragagoke genus (Leucobryum) plants, the Nisehabutaegoke genus (Leucophanes), Hosobaokinagoke (Leucobryum juniperoideum), Ooshiragagoke (Leucobryum scabrum), Arahashiragagoke (Leucobryum bowringii), it is preferable to use a Nisehabutaegoke (Leucophanes octoblepharioides), Hosobaokinagoke, Ooshiragagoke, the Arahashiragagoke The use is particularly preferable from the viewpoint of effectiveness.
これらシラガゴケ科植物を使用する際は、抽出物を用いるのが一般的である。抽出には、シラガゴケ科植物の胞子,胞子体,配偶体のいずれの部位を用いても構わないが、簡便に利用するには、胞子体と配偶体の全草を用いるとよい。抽出の際は、生のまま用いてもよいが、抽出効率を考えると、細切,乾燥,粉砕等の処理を行った後に抽出を行うことが好ましい。抽出は、抽出溶媒に浸漬するか、超臨界流体や亜臨界流体を用いた抽出方法でも行うことができる。抽出効率を上げるため、撹拌や抽出溶媒中でホモジナイズしてもよい。抽出温度としては、5℃程度から抽出溶媒の沸点以下の温度とするのが適切である。抽出時間は抽出溶媒の種類や抽出温度によっても異なるが、1時間〜14日間程度とするのが適切である。 When using these plant species, it is common to use an extract. For extraction, any of the spores, spore bodies, and gametophytes of the moss family may be used, but for easy use, the whole plant of spore bodies and gametophytes may be used. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days.
抽出溶媒としては、水の他、メタノール,エタノール,プロパノール,イソプロパノール等の低級アルコール、1,3−ブチレングリコール,プロピレングリコール,ジプロピレングリコール,グリセリン等の多価アルコール、エチルエーテル,プロピルエーテル等のエーテル類、酢酸ブチル,酢酸エチル等のエステル類、アセトン,エチルメチルケトン等のケトン類などの溶媒を用いることができ、これらより1種又は2種以上を選択して用いる。また、生理食塩水,リン酸緩衝液,リン酸緩衝生理食塩水等を用いてもよい。さらに、水や二酸化炭素,エチレン,プロピレン,エタノール,メタノール,アンモニアなどの1種又は2種以上の超臨界流体や亜臨界流体を用いてもよい。 Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, and ethers such as ethyl ether and propyl ether. , Solvents such as esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these are selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.
シラガゴケ科植物の上記溶媒による抽出物は、そのままでも使用することができるが、濃縮,乾固した物を水や極性溶媒に再度溶解したり、或いはこれらの生理作用を損なわない範囲で脱色,脱臭,脱塩等の精製処理を行ったり、カラムクロマトグラフィー等による分画処理を行った後に用いてもよい。シラガゴケ科植物の前記抽出物やその処理物及び分画物は、各処理及び分画後に凍結乾燥し、用時に溶媒に溶解して用いることもできる。 Extracts of the above-mentioned solvents of the moss family can be used as they are, but they are decolorized and deodorized as long as the concentrated and dried solids are dissolved again in water or a polar solvent, or the physiological functions thereof are not impaired. It may be used after a purification treatment such as desalting or a fractionation treatment by column chromatography or the like. The above-mentioned extract of the moss family and its treated products and fractions can be freeze-dried after each treatment and fractionation and dissolved in a solvent at the time of use.
シラガゴケ科植物の抽出物は、優れた細胞賦活作用、美白作用、ヒアルロン酸産生促進作用、コラーゲン産生促進作用、抗酸化作用を有し、細胞賦活剤、美白剤、ヒアルロン酸産生促進剤、コラーゲン産生促進剤、抗酸化剤として利用することができる。 The extract of the moss plant has excellent cell activation, whitening, hyaluronic acid production promotion, collagen production promotion, antioxidant activity, cell activator, whitening agent, hyaluronic acid production promoter, collagen production It can be used as an accelerator and an antioxidant.
シラガゴケ科植物の抽出物を有効成分とする細胞賦活剤は、種々の細胞に対して優れた賦活作用を発揮するが、特に表皮細胞に対して優れた効果を発揮する。 A cell activator comprising an extract of a scallop plant as an active ingredient exerts an excellent activation action on various cells, but particularly exhibits an excellent effect on epidermal cells.
シラガゴケ科植物の抽出物を有効成分とする美白剤は、シミ・ソバカスといった色素沈着症状の改善に効果を発揮し、特にチロシナーゼ活性阻害に基づくメラニンの産生抑制に対して優れた効果を発揮する。 A whitening agent comprising an extract of a plant belonging to the moss family as an active ingredient is effective in improving pigmentation symptoms such as spots and freckles, and in particular, is excellent in suppressing melanin production based on inhibition of tyrosinase activity.
シラガゴケ科植物の抽出物を有効成分とするヒアルロン酸産生促進剤、コラーゲン産生促進剤は、真皮マトリックス成分の産生促進に優れた効果を発揮し、ヒアルロン酸やコラーゲンの産生促進に優れた効果を発揮する。 Hyaluronic acid production promoters and collagen production promoters, which are extracted from the moss family plant, are effective in promoting the production of dermal matrix components, and are effective in promoting the production of hyaluronic acid and collagen. To do.
シラガゴケ科植物の抽出物を有効成分とする抗酸化剤は、優れた抗酸化作用を発揮するが、特にフリーラジカル消去作用に優れた効果を発揮する。 Antioxidants containing an extract of the moss family as an active ingredient exhibit an excellent antioxidant effect, but exhibit an excellent effect particularly on free radical scavenging action.
また、シラガゴケ科植物の抽出物を皮膚外用剤に配合することにより、皮膚老化症状の防止・改善に優れた効果を発揮する老化防止改善用の皮膚外用剤を得ることができる。さらに、シラガゴケ科植物の抽出物は、健康維持や栄養補給を目的とするような食品や飲料にも用いることもできる。 Moreover, the skin external preparation for anti-aging improvement which exhibits the effect excellent in prevention and improvement of a skin aging symptom can be obtained by mix | blending the extract of the moss-family plant with an external preparation for skin. Furthermore, the extract of the moss family can also be used in foods and beverages for the purpose of maintaining health and providing nutrition.
シラガゴケ科植物の抽出物を皮膚外用剤に配合する際の配合量は、皮膚外用剤の種類や使用目的等によって調整することができるが、効果や安定性などの点から、全量に対して、0.0001〜50.0重量%が好ましく、より好ましくは、0.001〜20.0重量%である。 The blending amount of the extract of the plant belonging to the family Tricholomaceae can be adjusted according to the type of skin external preparation and the purpose of use, etc., but from the point of effect and stability, 0.0001-50.0 weight% is preferable, More preferably, it is 0.001-20.0 weight%.
シラガゴケ科植物の抽出物を配合する皮膚外用剤の剤型は任意であり、例えば、ローションなどの可溶化系、クリームや乳液などの乳化系,カラミンローション等の分散系として提供することができる。さらに、噴射剤と共に充填したエアゾール,軟膏剤,粉末,顆粒などの種々の剤型で提供することもできる。 The dosage form of the external preparation for skin to which the extract of the plant belonging to the moss family is blended is arbitrary, and can be provided as a solubilizing system such as lotion, an emulsifying system such as cream or emulsion, or a dispersing system such as calamine lotion. Furthermore, it can also be provided in various dosage forms such as aerosols, ointments, powders and granules filled with a propellant.
なお、シラガゴケ科植物の抽出物を配合する皮膚外用剤には、シラガゴケ科植物の抽出物の他に、必要に応じて、通常医薬品,医薬部外品,皮膚化粧料,毛髪用化粧料及び洗浄料に配合される、油性成分,保湿剤,粉体,色素,乳化剤,可溶化剤,洗浄剤,紫外線吸収剤,増粘剤,薬剤,香料,樹脂,防菌防黴剤,アルコール類等を適宜配合することができる。また、本発明の効果を損なわない範囲において、他の細胞賦活剤、美白剤、ヒアルロン酸産生促進剤、コラーゲン産生促進剤、抗酸化剤との併用も可能である。 In addition, for the topical skin preparation containing the extract of the moss family, in addition to the extract of the moss family, if necessary, it is usually a pharmaceutical product, quasi-drug, skin cosmetic, hair cosmetic and washing. Oil components, moisturizers, powders, pigments, emulsifiers, solubilizers, cleaning agents, UV absorbers, thickeners, drugs, fragrances, resins, antibacterial and antifungal agents, alcohols, etc. It can mix | blend suitably. In addition, other cell activators, whitening agents, hyaluronic acid production promoters, collagen production promoters and antioxidants can be used in combination as long as the effects of the present invention are not impaired.
以下に、シラガゴケ科植物の抽出物の製造例、各作用を評価するための試験、皮膚外用剤としての処方例、使用試験についてさらに詳細に説明するが、本発明の技術的範囲はこれによってなんら限定されるものではない。まず、シラガゴケ科植物の抽出物の製造例を示す。 In the following, production examples of extracts of the moss family, tests for evaluating each action, formulation examples as external preparations for skin use, and use tests will be described in more detail, but the technical scope of the present invention is not limited thereby. It is not limited. First, an example of producing an extract of a plant belonging to the family Shiragagoceae is shown.
[製造例1]
シラガゴケ科植物の全草の乾燥粉砕物1kgに50重量%エタノール水溶液を10リットル加え、室温で7日間浸漬した。抽出液をろ過して回収し、溶媒を除去した後、シラガゴケ科植物抽出物を得た。
[Production Example 1]
10 liters of a 50 wt% ethanol aqueous solution was added to 1 kg of a dry pulverized whole plant of the moss family and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, an extract of a plant belonging to the family Shiragagoceae was obtained.
[製造例2]
シラガゴケ科植物の全草の乾燥粉砕物1kgに水を9リットル加え、90℃にて6時間還流して抽出した。抽出液をろ過して回収し、溶媒を除去した後、シラガゴケ科植物抽出物を得た。
[Production Example 2]
Nine liters of water was added to 1 kg of a dried pulverized whole plant of the moss, and extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration, and after removing the solvent, an extract of a plant belonging to the family Shiragagoceae was obtained.
[製造例3]
シラガゴケ科植物の全草の乾燥粉砕物1kgにメタノールを9リットル加え、室温で7日間浸漬した。抽出液をろ過して回収し、溶媒を除去した後、シラガゴケ科植物抽出物を得た。
[Production Example 3]
Nine liters of methanol was added to 1 kg of dried pulverized whole grass of the moss family and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, an extract of a plant belonging to the family Shiragagoceae was obtained.
[製造例4]
超臨界抽出装置にシラガゴケ科植物の全草を投入し、40℃において15MPaの気圧下で二酸化炭素の超臨界流体を用いて抽出した。抽出物を回収し、シラガゴケ科植物抽出物を得た。
[Production Example 4]
The whole plant of the moss was introduced into a supercritical extraction apparatus and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under a pressure of 15 MPa. The extract was recovered to obtain an extract of the plant belonging to the family Asteraceae.
次に、シラガゴケ科植物抽出物の表皮細胞の賦活作用について示す。試料には、ホソバオキナゴケ及びオオシラガゴゲより製造例1を用いて抽出したホソバオキナゴケ抽出物及びオオシラガゴゲ抽出物を用いた。 Next, it shows about the activation effect | action of the epidermis cell of the mosquito family plant extract. As the sample, the extract of Hosobakinagoga and Ooshiragagoge extracted from Production Example 1 from Hosobakinagoga and Oshiragagoge was used.
評価は、以下の手順で行った。正常ヒト表皮細胞を1ウェル当たり2.0×104個となるように96穴マイクロプレートに播種した。播種培地には、ダルベッコ改変イーグル培地(DMEM)に1%のウシ胎児血清を添加したものを用いた。24時間培養後、任意の濃度の試料を添加した試験培地に交換し、さらに48時間培養した。次いで3−(4,5−ジメチル−2−チアゾリル)−2,5−ジフェニルテトラゾリウムブロミド(MTT)を400μg/mL含有する培地に交換して2時間培養し、テトラゾリウム環の開環により生じるフォルマザンを2−プロパノールにて抽出し、マイクロプレートリーダーにて550nmの吸光度を測定した。同時に濁度として650nmにおける吸光度を測定し、両測定値の差により細胞賦活作用を評価した。評価結果を、試料無添加のブランクにおける細胞賦活作用を100とした相対値にて表1に示す。なお、表中の*及び**は、t検定における有意確率P値に対し、有意確率5%未満(P<0.05)を*で、有意確率1%未満(P<0.01)を**で表したものであり、−は評価を行っていないことを示している。 The evaluation was performed according to the following procedure. Normal human epidermal cells were seeded in a 96-well microplate at 2.0 × 10 4 cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum. After culturing for 24 hours, the culture medium was replaced with a test medium to which a sample having an arbitrary concentration was added, and further cultured for 48 hours. Next, the medium containing 400 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was exchanged and cultured for 2 hours. Formazan produced by the opening of the tetrazolium ring was removed. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in Table 1 as relative values with the cell activation effect in the blank with no sample as 100. In the table, * and ** indicate a significance probability of less than 5% (P <0.05) with respect to the significance probability P value in the t test, and a significance probability of less than 1% (P <0.01). It is represented by **, and-indicates that evaluation is not performed.
表1より明らかなように、ホソバオキナゴケ抽出物及びオオシラガゴゲ抽出物を添加したそれぞれの培地において、有意な表皮細胞賦活作用が認められた。特に、ホソバオキナゴケ抽出物を0.063〜0.125mg/mL、オオシラガゴゲ抽出物を0.25〜0.5mg/mL添加した場合には、ブランクと比較して、危険率1%未満で有意な表皮細胞賦活作用が認められた。このことから、ホソバオキナゴケ抽出物及びオオシラガゴゲ抽出物は、優れた表皮細胞賦活作用を有することが明らかとなった。 As is clear from Table 1, a significant epidermal cell activating effect was observed in each medium to which the extract of the red moss moth and the extract of the white moth was added. In particular, when 0.063 to 0.125 mg / mL of Hosobakinago Extract and 0.25 to 0.5 mg / mL of Oshiragagoge Extract are added, the epidermis is significant with a risk rate of less than 1% compared to the blank. A cell activation effect was observed. From this, it has been clarified that the moss extract and the white moth moth extract have an excellent epidermal cell activation action.
次に、シラガゴケ科植物抽出物のメラニン産生抑制作用の評価を示す。試料には、アラハシラガゴケ及びオオシラガゴゲより製造例1を用いて抽出したアラハシラガゴケ抽出物及びオオシラガゴゲ抽出物を用いた。 Next, the evaluation of the melanin production inhibitory action of the extract of the plant belonging to the family Tricholomaceae is shown. As the sample, the arahashiragagoke extract and the osillagagoge extract extracted from arahashiragagoke and ooshiragagoge using the production example 1 were used.
評価は、以下の手順で行った。B16マウスメラノーマF0ストレイン(B16F0)細胞を35mmディッシュに1ディッシュあたり2000個にて播種した。24時間培養後、任意の濃度の試料を添加した5重量%ウシ胎児血清(FCS)添加ダルベッコ改変イーグル培地(DMEM)に交換した。7日間培養後、0.25%トリプシンを用いて細胞を回収し、1.5mLマイクロチューブに移して遠心操作して細胞沈殿物を得た。さらに、試料を添加した培地の代わりに、ダルベッコ改変イーグル培地(DMEM)に5重量%ウシ胎児血清(FCS)を添加したものをブランクとし、同様に目視判定を行った。また、目視判定は、表2に示す通り、5段階評価によって行った。表3に判定の結果を示した。 The evaluation was performed according to the following procedure. B16 mouse melanoma F0 strain (B16F0) cells were seeded in a 35 mm dish at 2000 cells per dish. After culturing for 24 hours, the culture medium was replaced with Dulbecco's modified Eagle medium (DMEM) supplemented with 5 wt% fetal calf serum (FCS) to which a sample of an arbitrary concentration was added. After culturing for 7 days, the cells were collected using 0.25% trypsin, transferred to a 1.5 mL microtube, and centrifuged to obtain a cell precipitate. Furthermore, instead of the medium to which the sample was added, Dulbecco's modified Eagle medium (DMEM) to which 5 wt% fetal calf serum (FCS) was added was used as a blank, and visual determination was similarly performed. Further, as shown in Table 2, the visual judgment was performed by a five-step evaluation. Table 3 shows the determination results.
表3より明らかなように、アラハシラガゴケ抽出物及びオオシラガゴゲ抽出物を添加した培地を用いた場合に、有意なメラニン産生抑制作用が認められた。特に、アラハシラガゴケ抽出物及びオオシラガゴゲ抽出物を0.25〜0.5mg/mL添加した場合には、ほとんど黒化は認められなかった。このことから、アラハシラガゴケ抽出物及びオオシラガゴゲ抽出物は、メラニン産生抑制作用に基づく高い美白作用を有することが明らかとなった。 As is clear from Table 3, when a medium supplemented with Arahashiragagoke extract and Oshiragagoge extract was used, a significant melanin production inhibitory effect was observed. In particular, almost no blackening was observed when 0.25 to 0.5 mg / mL of Arahashiragagoke extract and Oshiragagoge extract were added. From this, it has been clarified that Arahashiragagoke extract and Oshiragagoge extract have a high whitening action based on a melanin production inhibitory action.
次に、シラガゴケ科植物抽出物のヒアルロン酸産生促進作用の評価を示す。試料には、ホソバオキナゴケより製造例1を用いて抽出したホソバオキナゴケ抽出物を用いた。 Next, evaluation of the hyaluronic acid production promoting action of the extract of the plant belonging to the family Labylaceae is shown. As the sample, the extract of Hosobaokinago using the production example 1 was used.
評価は、以下の手順で行った。正常ヒト真皮線維芽細胞を1ウェル当たり2.0×104個となるように96穴マイクロプレートに播種し、任意の濃度の試料を添加した0.5重量%牛胎仔血清添加ダルベッコ修正基礎培地(DMEM)にて37℃で5日間培養し、培養上清のヒアルロン酸量をEnzyme−linked immunosorbent assay(ELISA)法により測定した。同時に線維芽細胞数を計測し、細胞当たりのヒアルロン酸産生量を算出して、試料を含有しないブランクの細胞当たりのヒアルロン酸産生量を100とした相対値にて表4に示した。なお、表中の**は、t検定における有意確率P値に対し、有意確率1%未満(P<0.01)のものを表したものである。 The evaluation was performed according to the following procedure. Dulbecco's modified basal medium supplemented with 0.5% by weight fetal calf serum, in which normal human dermal fibroblasts are seeded in a 96-well microplate at 2.0 × 10 4 per well, and a sample of an arbitrary concentration is added (DMEM) was cultured at 37 ° C. for 5 days, and the amount of hyaluronic acid in the culture supernatant was measured by the enzyme-linked immunosorbent assay (ELISA) method. At the same time, the number of fibroblasts was counted, the amount of hyaluronic acid produced per cell was calculated, and the relative value with the amount of hyaluronic acid produced per blank cell containing no sample as 100 was shown in Table 4. In addition, ** in the table represents a significance probability less than 1% (P <0.01) with respect to the significance probability P value in the t test.
表4より明らかなように、ホソバオキナゴケ抽出物を添加した場合に、未添加の場合と比較して、有意なヒアルロン酸産生促進作用が認められた。特に、ホソバオキナゴケ抽出物を7.8〜62.5μg/mL添加した場合には、危険率1%未満で有意なヒアルロン酸産生促進作用が認められた。このことから、ホソバオキナゴケ抽出物は、優れたヒアルロン酸産生促進作用を有することが明らかとなった。 As can be seen from Table 4, when the moss extract was added, a significant hyaluronic acid production promoting effect was observed compared to the case where it was not added. In particular, when 7.8-62.5 μg / mL of Hosobakinago Extract was added, a significant hyaluronic acid production promoting action was observed with a risk rate of less than 1%. From this, it was clarified that Hosobakinagoki extract has an excellent hyaluronic acid production promoting action.
次に、シラガゴケ科植物抽出物のコラーゲン産生促進作用の評価を示す。試料には、ホソバオキナゴケ及びオオシラガゴゲより製造例1を用いて抽出したホソバオキナゴケ抽出物及びオオシラガゴゲ抽出物を用いた。 Next, the evaluation of the collagen production promoting action of the extract of the plant belonging to the family Labylaceae is shown. As the sample, the extract of Hosobakinagoga and Ooshiragagoge extracted from Production Example 1 from Hosobakinagoga and Oshiragagoge was used.
評価は、以下の手順で行った。正常ヒト表皮細胞を1ウェル当たり2.0×104個となるように96穴マイクロプレートに播種し、試料を任意の濃度添加した1.0重量%牛胎仔血清添加ダルベッコ修正基礎培地(DMEM)にて37℃で24時間培養し、培養上清のコラーゲン量をELISA法により測定した。同時に線維芽細胞数を計測し、細胞当たりのコラーゲン産生量を算出して、試料を含有しないブランクの細胞当たりのコラーゲン産生量を100とした相対値にて表5に示した。なお、表中の**は、t検定における有意確率P値に対し、有意確率1%未満(P<0.01)のものを表したものである。 The evaluation was performed according to the following procedure. Dulbecco's modified basal medium (DMEM) supplemented with 1.0 wt% fetal bovine serum to which normal human epidermal cells were seeded in a 96-well microplate at 2.0 × 10 4 cells per well, and an arbitrary concentration of the sample was added. And cultured at 37 ° C. for 24 hours, and the amount of collagen in the culture supernatant was measured by ELISA. At the same time, the number of fibroblasts was counted, the amount of collagen production per cell was calculated, and the values are shown in Table 5 as relative values with the amount of collagen production per blank cell containing no sample as 100. In addition, ** in the table represents a significance probability less than 1% (P <0.01) with respect to the significance probability P value in the t test.
表5より明らかなように、ホソバオキナゴケ抽出物及びオオシラガゴゲ抽出物を添加したそれぞれの培地において、未添加の場合と比較して、有意なコラーゲン産生促進作用が認められた。特に、ホソバオキナゴケ抽出物及びオオシラガゴゲ抽出物を0.031〜0.125mg/mL添加した場合には、危険率1%未満で有意なコラーゲン産生促進作用が認められた。このことから、ホソバオキナゴケ抽出物及びオオシラガゴゲ抽出物は、優れたコラーゲン産生促進作用を有することが明らかとなった。 As is clear from Table 5, in each medium to which the extract of Hosobakichinago and Oshiragagoge extract was added, a significant collagen production promoting action was recognized as compared with the case where it was not added. In particular, when 0.031 to 0.125 mg / mL of Hosobakichinago extract and Oshiragagoge extract were added, a significant collagen production promoting action was observed with a risk rate of less than 1%. From this, it was clarified that Hosobakinagoki extract and Oshiragagoge extract have an excellent collagen production promoting action.
次に、シラガゴケ科植物抽出物の抗酸化作用について示す。試料には、ホソバオキナゴケ、オオシラガゴゲ、アラハシラガゴケより製造例1を用いて抽出したホソバオキナゴケ抽出物、オオシラガゴゲ抽出物、及びアラハシラガゴケ抽出物を用いた。 Next, the antioxidant action of the extract of the plant belonging to the family Shiragagoceae is shown. As the sample, the mosquito extract, the mosquito moth extract, and the mosquito moth extract extracted from Production Example 1 were used.
評価は、以下の手順で行った。50重量%エタノール水溶液にて1mg/mLの濃度に希釈した試料溶液を96穴マイクロプレートに100μL添加した。次に、0.2mMの濃度になるようにエタノールにて調製した1,1−ジフェニル−2−ピクリルヒドラジル(DPPH)溶液を96穴マイクロプレートに100μL添加した。攪拌しながら暗所に放置し、24時間後に516nmの吸光度を測定した。試料が無添加のブランクの吸光度を(A)、試料を添加したときの吸光度を(B)としたとき、式(1)の値をラジカル消去率とした。評価結果を表6に示した。
式(1) {1−(B)/(A)}×100(%)
The evaluation was performed according to the following procedure. 100 μL of a sample solution diluted to a concentration of 1 mg / mL with a 50 wt% aqueous ethanol solution was added to a 96-well microplate. Next, 100 μL of a 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared with ethanol to a concentration of 0.2 mM was added to a 96-well microplate. The mixture was left in the dark with stirring, and the absorbance at 516 nm was measured after 24 hours. When the absorbance of the blank with no sample added was (A) and the absorbance when the sample was added was (B), the value of formula (1) was defined as the radical elimination rate. The evaluation results are shown in Table 6.
Formula (1) {1- (B) / (A)} × 100 (%)
表6より明らかなように、ホソバオキナゴケ抽出物、オオシラガゴゲ抽出物、及びアラハシラガゴケ抽出物は、優れた抗酸化作用を有することが分かった。 As is apparent from Table 6, it was found that the mosquito extract, the mosquito extract, and the Araha moss extract have an excellent antioxidant effect.
続いて、本発明に係るシラガゴケ科植物抽出物を配合した皮膚外用剤の処方例を示す。 Then, the prescription example of the skin external preparation which mix | blended the Shiragagoceae plant extract which concerns on this invention is shown.
[処方例1]乳液
(1)スクワラン 10.0(重量%)
(2)メチルフェニルポリシロキサン 4.0
(3)水素添加パーム核油 0.5
(4)水素添加大豆リン脂質 0.1
(5)モノステアリン酸ポリオキシエチレン
ソルビタン(20E.O.) 1.3
(6)モノステアリン酸ソルビタン 1.0
(7)グリセリン 4.0
(8)パラオキシ安息香酸メチル 0.1
(9)カルボキシビニルポリマー 0.15
(10)精製水 53.85
(11)アルギニン(1重量%水溶液) 20.0
(12)ホソバオキナゴケ抽出物[製造例1] 5.0
製法:(1)〜(6)の油相成分を80℃にて加熱溶解する。一方(7)〜(10)の水相成分を80℃にて加熱溶解する。これに前記油相成分を攪拌しながら加え、ホモジナイザーにより均一に乳化する。乳化終了後、冷却を開始し、(11)と(12)を順次加え、均一に混合する。
[Formulation Example 1] Emulsion (1) Squalane 10.0 (wt%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 53.85
(11) Arginine (1 wt% aqueous solution) 20.0
(12) Hosobakinago Extract [Production Example 1] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix uniformly.
[処方例2]化粧水
(1)エタノール 15.0(重量%)
(2)ポリオキシエチレン(40E.O.)硬化ヒマシ油 0.3
(3)香料 0.1
(4)精製水 78.38
(5)クエン酸 0.02
(6)クエン酸ナトリウム 0.1
(7)グリセリン 1.0
(8)ヒドロキシエチルセルロース 0.1
(9)ホソバオキナゴケ抽出物[製造例3] 5.0
製法:(1)に(2)及び(3)を溶解する。溶解後、(4)〜(8)を順次添加した後、十分に攪拌し、(9)を加え、均一に混合する。
[Prescription Example 2] Lotion (1) Ethanol 15.0 (% by weight)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 78.38
(5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Hosobaokinago extract [Production Example 3] 5.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.
[処方例3]クリーム
(1)スクワラン 10.0(重量%)
(2)ステアリン酸 2.0
(3)水素添加パーム核油 0.5
(4)水素添加大豆リン脂質 0.1
(5)セタノール 3.6
(6)親油型モノステアリン酸グリセリン 2.0
(7)グリセリン 10.0
(8)パラオキシ安息香酸メチル 0.1
(9)アルギニン(20重量%水溶液) 15.0
(10)精製水 40.7
(11)カルボキシビニルポリマー(1重量%水溶液) 15.0
(12)ホソバオキナゴケ抽出物[製造例1] 1.0
製法:(1)〜(6)の油相成分を80℃にて加熱溶解する。一方(7)〜(10)の水相成分を80℃にて加熱溶解する。これに前記油相成分を攪拌しながら加え、ホモジナイザーにより均一に乳化する。乳化終了後、(11)を加え、冷却を開始し、40℃にて(12)を加え、均一に混合する。
[Prescription Example 3] Cream (1) Squalane 10.0 (% by weight)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20% by weight aqueous solution) 15.0
(10) Purified water 40.7
(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Hosobaokinago extract [Production Example 1] 1.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification is completed, add (11), start cooling, add (12) at 40 ° C., and mix uniformly.
[処方例4]美容液
(1)精製水 27.45(重量%)
(2)グリセリン 10.0
(3)ショ糖脂肪酸エステル 1.3
(4)カルボキシビニルポリマー(1重量%水溶液) 17.5
(5)アルギン酸ナトリウム(1重量%水溶液) 15.0
(6)モノラウリン酸ポリグリセリル 1.0
(7)マカデミアナッツ油脂肪酸フィトステリル 3.0
(8)N-ラウロイル-L-グルタミン酸
ジ(フィトステリル−2−オクチルドデシル) 2.0
(9)硬化パーム油 2.0
(10)スクワラン(オリーブ由来) 1.0
(11)ベヘニルアルコール 0.75
(12)ミツロウ 1.0
(13)ホホバ油 1.0
(14)1,3−ブチレングリコール 10.0
(15)L−アルギニン(10重量%水溶液) 2.0
(16)オオシラガゴゲ抽出物[製造例1] 5.0
製法:(1)〜(6)の水相成分を混合し、75℃にて加熱溶解する。一方、(7)〜(14)の油相成分を混合し、75℃にて加熱溶解する。次いで、上記水相成分に油相成分を添加して予備乳化を行った後、ホモミキサーにて均一に乳化する。乳化終了後に冷却を開始し、50℃にて(15)を加える。さらに40℃まで冷却し、(16)を加え、均一に混合する。
[Formulation Example 4] Cosmetic liquid (1) Purified water 27.45 (% by weight)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1 wt% aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by weight aqueous solution) 2.0
(16) White-winged extract [Production Example 1] 5.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C, add (16) and mix evenly.
[処方例5]水性ジェル
(1)カルボキシビニルポリマー 0.5(重量%)
(2)精製水 86.7
(3)水酸化ナトリウム(10重量%水溶液) 0.5
(4)エタノール 10.0
(5)パラオキシ安息香酸メチル 0.1
(6)香料 0.1
(7)ホソバオキナゴケ抽出物[製造例4] 2.0
(8)ポリオキシエチレン(60E.O.)硬化ヒマシ油 0.1
製法:(1)を(2)に加え、均一に攪拌した後、(3)を加える。均一に攪拌した後,(4)に予め溶解した(5)を加える。均一に攪拌した後、予め混合しておいた(6)〜(8)を加え、均一に攪拌混合する。
[Formulation Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (% by weight)
(2) Purified water 86.7
(3) Sodium hydroxide (10% by weight aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Hosobaokinago extract [Production Example 4] 2.0
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, add (5) previously dissolved in (4). After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.
[処方例6]クレンジング料
(1)スクワラン 81.0(重量%)
(2)イソステアリン酸ポリオキシエチレングリセリル 15.0
(3)精製水 3.0
(4)オオシラガゴゲ抽出物[製造例4] 1.0
製法:(1)と(2)を均一に溶解する。これに、(3)と(4)を順次加え、均一に混合する。
[Formulation Example 6] Cleansing Fee (1) Squalane 81.0 (wt%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Purified water 3.0
(4) White-winged extract [Production Example 4] 1.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.
[処方例7]洗顔フォーム
(1)ステアリン酸 16.0(重量%)
(2)ミリスチン酸 16.0
(3)親油型モノステアリン酸グリセリン 2.0
(4)グリセリン 20.0
(5)水酸化ナトリウム 7.5
(6)ヤシ油脂肪酸アミドプロピルベタイン 1.0
(7)精製水 36.5
(8)アラハシラガゴケ抽出物[製造例3] 1.0
製法:(1)〜(4)の油相成分を80℃にて加熱溶解する。一方(5)〜(7)の水相成分を80℃にて加熱溶解し、油相成分と均一に混合撹拌する。冷却を開始し、40℃にて(8)を加え、均一に混合する。
[Prescription Example 7] Face-wash foam (1) Stearic acid 16.0 (% by weight)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 36.5
(8) Arahashiragago extract [Production Example 3] 1.0
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.
[処方例8]メイクアップベースクリーム
(1)スクワラン 10.0(重量%)
(2)セタノール 2.0
(3)グリセリントリ−2−エチルヘキサン酸エステル 2.5
(4)親油型モノステアリン酸グリセリル 1.0
(5)プロピレングリコール 11.0
(6)ショ糖脂肪酸エステル 1.3
(7)精製水 69.4
(8)酸化チタン 1.0
(9)ベンガラ 0.1
(10)黄酸化鉄 0.4
(11)香料 0.1
(12)アラハシラガゴケ抽出物[製造例2] 1.2
製法:(1)〜(4)の油相成分を混合し、75℃にて加熱溶解する。一方、(5)〜(7)の水相成分を混合し、75℃にて加熱溶解し、これに(8)〜(10)の顔料を加え、ホモミキサーにて均一に分散させる。この水相成分に前記油相成分を加え、ホモミキサーにて乳化する。乳化終了後に冷却を開始し、40℃にて(11)と(12)の成分を加え、均一に混合する。
[Prescription Example 8] Make-up base cream (1) Squalane 10.0 (% by weight)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 69.4
(8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Arahashiragagoke extract [Production Example 2] 1.2
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.
[処方例9]乳液状ファンデーション
(1)メチルポリシロキサン 2.0(重量%)
(2)スクワラン 5.0
(3)ミリスチン酸オクチルドデシル 5.0
(4)セタノール 1.0
(5)ポリオキシエチレン(20E.O.)
ソルビタンモノステアリン酸エステル 1.3
(6)モノステアリン酸ソルビタン 0.7
(7)1,3−ブチレングリコール 8.0
(8)キサンタンガム 0.1
(9)パラオキシ安息香酸メチル 0.1
(10)精製水 57.4
(11)酸化チタン 9.0
(12)タルク 7.4
(13)ベンガラ 0.5
(14)黄酸化鉄 1.1
(15)黒酸化鉄 0.1
(16)香料 0.1
(17)ニセハブタエゴケ抽出物[製造例4] 1.0
製法:(1)〜(6)の油相成分を混合し、75℃にて加熱溶解する。一方、(7)〜(10)の水相成分を混合し、75℃にて加熱溶解し、これに(11)〜(15)の顔料を加え、ホモミキサーにて均一に分散する。油相成分を加え、乳化を行う。乳化終了後に冷却を開始し、40℃にて(16)と(17)の成分を順次加え、均一に混合する。
[Formulation Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (wt%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Purified water 57.4
(11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Fake lobster extract [Production Example 4] 1.0
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.
[処方例10]油中水型エモリエントクリーム
(1)流動パラフィン 30.0(重量%)
(2)マイクロクリスタリンワックス 2.0
(3)ワセリン 5.0
(4)ジグリセリンオレイン酸エステル 5.0
(5)塩化ナトリウム 1.3
(6)塩化カリウム 0.1
(7)プロピレングリコール 3.0
(8)1,3−ブチレングリコール 5.0
(9)パラオキシ安息香酸メチル 0.1
(10)ニセハブタエゴケ抽出物[製造例1] 1.0
(11)精製水 47.4
(12)香料 0.1
製法:(5)と(6)を(11)の一部に溶解して50℃とし、50℃に加熱した(4)に撹拌しながら徐々に加える。これを混合した後、70℃にて加熱溶解した(1)〜(3)に均一に分散する。これに(7)〜(10)を(11)の残部に70℃にて加熱溶解したものを撹拌しながら加え、ホモミキサーにて乳化する。乳化終了後に冷却を開始し、40℃にて(12)を加え、均一に混合する。
[Formulation Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Fake lobster extract [Production Example 1] 1.0
(11) Purified water 47.4
(12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. while stirring, and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.
[処方例11]パック
(1)精製水 58.9(重量%)
(2)ポリビニルアルコール 12.0
(3)エタノール 17.0
(4)グリセリン 5.0
(5)ポリエチレングリコール(平均分子量1000) 2.0
(6)ホソバオキナゴケ抽出物[製造例2] 5.0
(7)香料 0.1
製法:(2)と(3)を混合し、80℃に加温した後、80℃に加温した(1)に溶解する。均一に溶解した後、(4)と(5)を加え、攪拌しながら冷却を開始する。40℃まで冷却し、(6)と(7)を加え、均一に混合する。
[Prescription Example 11] Pack (1) Purified water 58.9 (% by weight)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 5.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Hosobaokinago extract [Production Example 2] 5.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.
[処方例12]入浴剤
(1)香料 0.3(重量%)
(2)ホソバオキナゴケ抽出物[製造例1] 1.0
(3)炭酸水素ナトリウム 50.0
(4)硫酸ナトリウム 48.7
製法:(1)〜(4)を均一に混合する。
[Prescription Example 12] Bath agent (1) Fragrance 0.3 (% by weight)
(2) Hosobakinago Extract [Production Example 1] 1.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 48.7
Production method: (1) to (4) are mixed uniformly.
[処方例13]ヘアーワックス
(1)ステアリン酸 3.0(重量%)
(2)マイクロクリスタリンワックス 2.0
(3)セチルアルコール 3.0
(4)高重合メチルポリシロキサン 2.0
(5)メチルポリシロキサン 5.0
(6)ポリ(オキシエチレン・オキシプロピレン)
メチルポリシロキサン共重合体 1.0
(7)パラオキシ安息香酸メチル 0.1
(8)1,3−ブチレングリコール 7.5
(9)アルギニン 0.7
(10)精製水 73.6
(11)オオシラガゴゲ抽出物[製造例1] 2.0
(12)香料 0.1
製法:(1)〜(6)の油相成分を混合し、75℃にて加熱溶解後する。一方、(7)〜(10)の水相成分を75℃にて加熱溶解し、前記油相成分を加え、ホモミキサーにて乳化する。乳化終了後に冷却を開始し、40℃にて(11)と(12)の成分を加え、均一に混合する。
[Prescription Example 13] Hair wax (1) Stearic acid 3.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 73.6
(11) White-winged extract [Production Example 1] 2.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.
[処方例14]ヘアートニック
(1)エタノール 50.0(重量%)
(2)精製水 48.9
(3)ホソバオキナゴケ抽出物[製造例3] 1.0
(4)香料 0.1
製法:(1)〜(4)の成分を混合,均一化する。
[Prescription Example 14] Hair artic (1) Ethanol 50.0 (% by weight)
(2) Purified water 48.9
(3) Hosobaokinago extract [Production Example 3] 1.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.
次に、シラガゴケ科植物抽出物を配合した処方を用いて使用試験を行い、シワ,タルミ,肌のハリ,シミ,クスミについて改善効果を評価した。その際、処方例1に示した乳液の処方に表7に記載するシラガゴケ科植物抽出物をそれぞれ配合し、実施例1〜6として使用試験を行った。また、シラガゴケ科植物抽出物を精製水に代替し、比較例1として同時に使用試験を行った。 Next, a use test was conducted using a prescription blended with an extract of the plant belonging to the family Shiragagagoceae, and the improvement effect was evaluated with respect to wrinkles, tarmi, skin firmness, spots, and kuzumi. At that time, each of the sphagnum plant extract described in Table 7 was blended with the emulsion formulation shown in Formulation Example 1, and a use test was conducted as Examples 1-6. Moreover, the use test was simultaneously performed as the comparative example 1 by substituting for the extract of the plant of the moss family for purified water.
各試料について、シワ,タルミ,肌のハリ,シミ,クスミといった症状が顕著に認められる40〜60才代の男女パネラー各20名をそれぞれ一群とし、ブラインドにて2カ月間使用させ、使用前後の皮膚状態の変化を観察して評価した。皮膚症状の指標として、シワ,タルミ,肌のハリ,シミ,クスミについて、「改善」,「やや改善」,「変化なし」の三段階で評価し、表8に各評価を得たパネラー数にて示した。 For each sample, each group of 20 male and female panelists in their 40s to 60s who have noticeable symptoms such as wrinkles, tarmi, skin firmness, spots, and kusumi are used as a group for 2 months. Changes in skin condition were observed and evaluated. As indicators of skin symptoms, wrinkles, tarmi, skin firmness, stains, and kusumi were evaluated in three stages: “Improved”, “Slightly improved”, and “No change”. Showed.
表8より、シワ,タルミ,肌のハリ,シミ,クスミについて、シラガゴケ科植物抽出物を含有しない比較例使用群においては、6割以上のパネラーに改善は認められなかったが、シラガゴケ科植物抽出物を配合した実施例使用群においては、6割以上のパネラーに明確な改善が認められた。 According to Table 8, for wrinkles, tarmi, skin firmness, stains, and kusumi, in the comparative use group that does not contain the extract of the plant of the family Shiragagoceae, improvement was not recognized in more than 60% of the panelists, In the example use group in which the product was blended, 60% or more of panelists clearly improved.
以上のように、本発明の実施例においては、従来の比較例よりも、シワ,タルミ,肌のハリ,シミ,クスミの改善に優れた効果を有していた。このことから、シラガゴケ科植物抽出物を配合した皮膚外用剤は、皮膚老化症状の防止・改善に優れた効果を発揮することが明らかとなった。
As described above, in the examples of the present invention, the effects of improving wrinkles, tarmi, skin firmness, spots, and stains were superior to those of the conventional comparative example. From this, it was clarified that the external preparation for skin containing the extract of the plant belonging to the family Tricholomaceae exhibits an excellent effect in preventing and improving skin aging symptoms.
Claims (6)
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