JP4018605B2 - Cell activating agent, whitening agent, antioxidant, and skin external preparation - Google Patents

Description

The present invention relates to a cell activator, a whitening agent, an antioxidant, and a skin external preparation. More specifically, the present invention relates to a cell activator, a whitening agent, an antioxidant, and a skin external preparation containing an extract of one or two or more kinds of plants selected from the family Grimmiaceae .

  Causes of aging symptoms such as wrinkles, blemishes and skin elasticity decrease due to aging and ultraviolet rays, etc., due to cell function decline, melanin production and pigmentation due to ultraviolet rays, decrease and degeneration of dermal matrix components such as hyaluronic acid and collagen, ultraviolet rays, etc. Examples include oxidative damage of cells. In order to prevent and ameliorate such aging symptoms, search for various active ingredients and formulation studies have been conventionally conducted. As the cell activator, the essence of Ponkan (see Patent Document 1), as the whitening agent, water and / or organic solvent extract of Hakutsuru Reishi (see Patent Document 2), and as the antioxidant, the genus Sarogase Plant extracts (see Patent Document 3) are known.

  In addition, the prior art regarding the cell activator, the whitening agent, the antioxidant, and the skin external preparation which use the extract of a Hostaceae plant as an active ingredient was not recognized.

JP 2001-131045 A JP 2003-89630 A Japanese Patent Laid-Open No. 10-182413

  Conventionally used cell activators, whitening agents, and antioxidants may be insufficient as essential effects, and development of better active ingredients has been expected. The present invention has been made in view of such conventional problems, and has been found to be an active ingredient that is naturally derived and highly safe, has an excellent cell activating action, whitening action, and antioxidant action. It aims at providing the skin external preparation useful for improvement.

  In order to solve the above-mentioned problems, the present inventors have studied various naturally-derived substances with respect to actions useful for preventing and improving aging. As a result, they found an excellent cell activation effect, whitening effect, and antioxidant effect in the extract of one or more plants selected from the host family, and further studies were made to complete the present invention. That is, this invention provides the cell activator, whitening agent, antioxidant, and skin external preparation which use the extract of the 1 type, or 2 or more types of plant of the Hostaceae family as an active ingredient.

  ADVANTAGE OF THE INVENTION According to this invention, the cell activator, whitening agent, and antioxidant which have the outstanding effect can be provided. In addition, by adding these to skin external preparations, skin external preparations and foods for improving aging prevention that exhibit excellent effects in the prevention and improvement of skin aging symptoms such as wrinkles, tarmi, skin firmness, stains, and scum The composition can be provided.

The plant used as the raw material of the present invention may be a plant of the family Grimmiaceae . The Giboushigoke plant belonging to the family, Yamato Haq butterflies moss genus (Campylostelium), Tsubanagoke genus (Coscinodon), Giboushigoke genus (Grimmia), Nejiregoke genus (Ptycomitrium), Shimofurigoke genus (Racomitrium), and the like are known Ms. Giboushigoke genus (Schistidium) . The Yamato Haq butterflies moss plants of the genus, Hizatsukigoke (Campylostelium saxicola), Yamato Haq butterflies moss (Campylostelium saxicola var. Brachycarpum) and the like are known. As a plant belonging to the genus Camellia, Coscinodon humilis and the like are known. The Giboushigoke plants of the genus, solani Giboushigoke (Grimmia affinis), tree Mi Giboushigoke (Grimmia apiculata), story Giboushigoke (Grimmia atrata), co-pungens Giboushigoke (Grimmia brachydictyon), Kosunagoke (Grimmia curvata), Takamine Giboushigoke (Grimmia donniana), Jari Giboushigoke (Grimmia elongata), red Giboushigoke (Grimmia funalis), histidinol lever Giboushigoke (Grimmia incurva), right wheel Giboushigoke (Grimmia mollis), Kofutagogoke (Grimmia percarinata), Quai Giboushigoke (Grimmia ilifera), co-pot Giboushigoke (Grimmia pulvinata), Blue Giboushigoke (Grimmia subsulcata), co-Oy Giboushigoke (Grimmia trichophylla var. muehlenbeckii) and the like are known. The Nejiregoke plants of the genus, Hachijiregoke (Ptycomitrium dentatum), Hidagoke (Ptycomitrium fauriei), Nagabachijiregoke (Ptycomitrium linearifolium), Shinachijiregoke (Ptycomitrium polyphylloides), Chijiregoke (Ptycomitrium sinense), etc. Kobanohidagoke (Ptycomitrium wilsonii) is known. The Shimofurigoke genus plant, Malva Sunagoke (Racomitrium aciculare), shuffle play Sunagoke (Racomitrium aquaticum), Kobanosunagoke (Racomitrium barbuloides), Sunagoke (Racomitrium canescens), Datura Sunagoke (Racomitrium carinatum), Haisunagoke (Racomitrium ericoides), Miyama Sunagoke (Racomitrium fasciculare), Nagaenosunagoke (Racomitrium fasciculare var. atroviride), Kurokawakigoke (Racomitrium heterostichum), Harisunagoke (Racomit ium heterostichum var. brachypodium), Ezosunagoke (Racomitrium japonicum), Shimofurigoke (Racomitrium lanuginosum), Tateyama Sunagoke (Racomitrium muticum), black Miyama Sunagoke (Racomitrium nipponicum), etc. Himesunagoke (Racomitrium sudeticum) it is known. As the water Giboushigoke plants of the genus, Giboushigoke (Schistidium apocarpum), Annaba Giboushigoke (Schistidium confertum), rice bar Giboushigoke (Schistidium liliputanum), Ushio Giboushigoke (Schistidium maritimum), Ms. Giboushigoke (Schistidium rivulare), e buckwheat Giboushigoke (Schistidium strictum), Cobo There is known, for example, Schistidium subconfertum .

Plants as a raw material used in the present invention is not particularly limited as long as Giboushigoke family plant, because such availability is relatively easy for and effectiveness, Shimofurigoke genus (Racomitrium) plants, Ms. Giboushigoke genus (Schistidium) Plants can be preferably used. Shimofurigoke genus (Racomitrium) plants, as water Giboushigoke genus (Schistidium) plants, Sunagoke (Racomitrium canescens), it is preferable to use a Giboushigoke (Schistidium apocarpum), particularly preferred from the viewpoint that the effectiveness of using Sunagoke.

  When using these Hostaceae plants, it is common to use an extract. For extraction, any part of the spores and gametophytes of the host family plant may be used, but for easy use, the whole plant of spores and gametophytes may be used. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, homogenization may be performed in stirring or an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days.

  Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, and ethers such as ethyl ether and propyl ether. , Solvents such as esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  The above-mentioned extract of the host family plant can be used as it is, but it is decolorized and deodorized as long as the concentrated and dried product is dissolved again in water or a polar solvent, or the physiological action thereof is not impaired. It may be used after a purification treatment such as desalting or a fractionation treatment by column chromatography or the like. The above-mentioned extract of the genus Phytoaceae plant, its treated product and fractionated product can be freeze-dried after each treatment and fractionation and dissolved in a solvent at the time of use.

  The extract of the plant belonging to the family Asteraceae has an excellent cell activation action, whitening action and antioxidant action, and can be used as a cell activator, whitening agent and antioxidant.

  A cell activator comprising an extract of the plant belonging to the genus Agrobiaceae as an active ingredient exerts an excellent activation effect on various cells, but particularly exhibits an excellent effect on dermal fibroblasts.

  A whitening agent containing an extract of the plant belonging to the family Aceraceae is effective for improving pigmentation symptoms such as spots and freckles, and is particularly effective for suppressing melanin production based on inhibition of tyrosinase activity.

  Antioxidants containing an extract of the plant belonging to the genus Phytoaceae as an active ingredient exhibit an excellent antioxidant action, and in particular, an excellent effect on free radical scavenging action.

  Moreover, the skin external preparation for the anti-aging improvement which exhibits the effect excellent in prevention and improvement of the skin aging symptom can be obtained by mix | blending the extract of the genus Agrobiaceae plant with a skin external preparation. Furthermore, the extract of the plant belonging to the family Asteraceae can also be used in foods and beverages for the purpose of maintaining health and providing nutrition.

  The blending amount of the extract of the host family plant in the external preparation for skin can be adjusted according to the type of skin external preparation and the purpose of use, etc., but from the point of effect and stability, 0.0001-50.0 weight% is preferable, More preferably, it is 0.001-20.0 weight%.

  The dosage form of the external preparation for skin containing the extract of the plant belonging to the genus Ceraminaceae is arbitrary, and for example, it can be provided as a solubilizing system such as lotion, an emulsifying system such as cream or emulsion, or a dispersing system such as calamine lotion. Furthermore, it can also be provided in various dosage forms such as aerosols, ointments, powders and granules filled with a propellant.

  In addition, the topical skin preparation containing the extract of the plant belonging to the family Asteraceae includes, in addition to the extract of the plant belonging to the family Asteraceae, as usual, pharmaceuticals, quasi-drugs, skin cosmetics, cosmetics for hair and washing. Oil components, moisturizers, powders, pigments, emulsifiers, solubilizers, cleaning agents, UV absorbers, thickeners, drugs, fragrances, resins, antibacterial and antifungal agents, alcohols, etc. It can mix | blend suitably. Moreover, in the range which does not impair the effect of this invention, combined use with another cell activator, whitening agent, and antioxidant is also possible.

  In the following, production examples of extracts of the plant belonging to the genus Agrobiaceae, tests for evaluating each action, formulation examples as external preparations for skin, and use tests will be described in more detail, but the technical scope of the present invention is not limited thereby. It is not limited. First, an example of production of an extract of the plant belonging to the family Aceraceae is shown.

[Production Example 1]
10 liters of a 50 wt% aqueous ethanol solution was added to 1 kg of a dry pulverized whole plant of the host family, and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, an extract of the plant belonging to the family Asteraceae was obtained.

[Production Example 2]
Nine liters of water was added to 1 kg of a dried pulverized whole plant of the genus Phytoaceae, and the mixture was extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration, and after removing the solvent, an extract of the plant belonging to the family Asteraceae was obtained.

[Production Example 3]
Nine liters of methanol was added to 1 kg of a dry pulverized whole plant of Hostaceae, and the mixture was immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, an extract of the plant belonging to the family Asteraceae was obtained.

[Production Example 4]
The whole plant of the host family was introduced into a supercritical extraction apparatus and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under a pressure of 15 MPa. The extract was collected to obtain an extract of the family Asteraceae.

  Next, it demonstrates about the activation effect | action of the dermal fibroblast of the host plant plant extract. As a sample, a snago extract extracted from snago using the production example 1 was used.

The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum. After culturing for 24 hours, the culture medium was replaced with a test medium to which a sample having an arbitrary concentration was added, and further cultured for 48 hours. Next, the medium containing 400 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was exchanged and cultured for 2 hours. Formazan produced by the opening of the tetrazolium ring was removed. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in Table 1 as relative values with the cell activation effect in the blank with no sample as 100. In addition, ** in the table represents a significance probability of less than 1% (P <0.01) with respect to the significance probability P value in the t test.

  As is clear from Table 1, a significant dermal fibroblast activation effect was observed in the medium supplemented with snago extract. In particular, when 0.125 to 0.5 mg / mL of Snagoke extract was added, a significant dermal fibroblast activation effect was observed with a risk rate of less than 1% compared to the blank. From this, it was clarified that the snago extract has an excellent dermal fibroblast activation effect.

  Next, the evaluation of the melanin production inhibitory action of the plant plant extract is shown. As a sample, a snago extract extracted from snago using the production example 1 was used.

  The evaluation was performed according to the following procedure. B16 mouse melanoma F0 strain (B16F0) cells were seeded in a 35 mm dish at 2000 cells per dish. After culturing for 24 hours, the culture medium was replaced with Dulbecco's modified Eagle medium (DMEM) supplemented with 5 wt% fetal calf serum (FCS) to which a sample of an arbitrary concentration was added. After culturing for 7 days, cells were collected using 0.25% trypsin, transferred to a 1.5 mL microtube and centrifuged to obtain a cell precipitate. Furthermore, instead of the medium to which the sample was added, a Dulbecco's modified Eagle medium (DMEM) to which 5 wt% fetal calf serum (FCS) was added was used as a blank, and visual determination was similarly performed. Further, as shown in Table 2, the visual judgment was performed by a five-step evaluation. Table 3 shows the determination results.

  As is clear from Table 3, when a medium supplemented with snago extract was used, a significant melanin production inhibitory effect was observed. In particular, no blackening was observed when 0.063 to 0.125 mg / mL of the snago extract was added. From this, it was clarified that the snago extract has a high whitening action based on a melanin production inhibitory action.

  Next, it shows about the antioxidant effect of the Agrobacterium plant extract. As a sample, a snago extract extracted from snago using the production example 1 was used.

The evaluation was performed according to the following procedure. 100 μL of a sample solution diluted to an arbitrary concentration with a 50 wt% aqueous ethanol solution was added to a 96-well microplate. Next, 100 μL of a 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared with ethanol to a concentration of 0.2 mM was added to a 96-well microplate. The mixture was left in the dark with stirring, and the absorbance at 516 nm was measured after 24 hours. When the absorbance of the blank with no sample added was (A) and the absorbance when the sample was added was (B), the value of formula (1) was defined as the radical elimination rate. The evaluation results are shown in Table 4.
Formula (1) {1- (B) / (A)} × 100 (%)

  As is apparent from Table 4, the snago extract was found to have an antioxidant effect.

  Then, the example of a prescription of the skin external preparation which mix | blended the Parameciumaceae plant extract which concerns on this invention is shown.

[Formulation Example 1] Emulsion (1) Squalane 10.0 (wt%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 53.85
(11) Arginine (1 wt% aqueous solution) 20.0
(12) Snagoke extract [Production Example 1] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix uniformly.

[Prescription Example 2] Lotion (1) Ethanol 15.0 (% by weight)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 78.38
(5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Snagoke extract [Production Example 3] 5.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.

[Prescription Example 3] Cream (1) Squalane 10.0 (% by weight)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20% by weight aqueous solution) 15.0
(10) Purified water 40.7
(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Snagoke extract [Production Example 1] 1.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification is completed, add (11), start cooling, add (12) at 40 ° C., and mix uniformly.

[Formulation Example 4] Cosmetic liquid (1) Purified water 27.45 (% by weight)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1 wt% aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by weight aqueous solution) 2.0
(16) Snagoke extract [Production Example 1] 5.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C, add (16) and mix evenly.

[Formulation Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (% by weight)
(2) Purified water 86.7
(3) Sodium hydroxide (10% by weight aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Snagoke extract [Production Example 4] 2.0
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, add (5) previously dissolved in (4). After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Formulation Example 6] Cleansing Fee (1) Squalane 81.0 (wt%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Purified water 3.0
(4) Snagoke extract [Production Example 4] 1.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.

[Prescription Example 7] Face-wash foam (1) Stearic acid 16.0 (% by weight)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 36.5
(8) Snagoke extract [Production Example 3] 1.0
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.

[Prescription Example 8] Make-up base cream (1) Squalane 10.0 (% by weight)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 69.4
(8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Snagoke extract [Production Example 2] 1.2
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Formulation Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (wt%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Purified water 57.4
(11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Snagoke extract [Production Example 4] 1.0
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Formulation Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Snagoke extract [Production Example 1] 1.0
(11) Purified water 47.4
(12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. while stirring and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.

[Prescription Example 11] Pack (1) Purified water 58.9 (% by weight)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 5.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Snagoke extract [Production Example 2] 5.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.

[Prescription Example 12] Bath agent (1) Fragrance 0.3 (% by weight)
(2) Snagoke extract [Production Example 1] 1.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 48.7
Production method: (1) to (4) are mixed uniformly.

[Prescription Example 13] Hair wax (1) Stearic acid 3.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 73.6
(11) Snagoke extract [Production Example 1] 2.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Prescription Example 14] Hair artic (1) Ethanol 50.0 (% by weight)
(2) Purified water 48.9
(3) Snagoke extract [Production Example 3] 1.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

  Next, a use test was conducted using a prescription containing an extract of the plant belonging to the family Aceraceae, and the improvement effect was evaluated for wrinkles, tarmi, skin firmness, spots, and kuzumi. At that time, each of the extracts of the plant belonging to the family Aceraceae listed in Table 5 was added to the formulation of the emulsion shown in Formulation Example 1, and the use test was conducted as Examples 1-6. In addition, a test for use was conducted simultaneously as Comparative Example 1 by substituting the purified plant water for the extract of the plant belonging to the family Aceraceae.

  For each sample, a group of 20 male and female panelists in their 40s to 60s who have noticeable symptoms such as wrinkles, tarmi, skin firmness, spots, and kusumi are used as a group for 2 months. Changes in skin condition were observed and evaluated. As an index of skin symptoms, wrinkles, tarmi, skin firmness, spots, and kusumi were evaluated in three stages: “Improved”, “Slightly improved”, and “No change”. Showed.

  According to Table 6, for wrinkles, tarmi, skin firmness, stains, and kusumi, in the comparative example use group that does not contain the extract of the plant belonging to the family Asteraceae, 70% or more of panelists did not improve, In the example use group in which the product was blended, 60% or more of panelists clearly improved.

  As described above, in the examples of the present invention, the effects of improving wrinkles, tarmi, skin firmness, spots, and stains were superior to those of the conventional comparative example. From this, it became clear that the skin external preparation which mix | blended the host plant plant extract exhibits the effect excellent in prevention and improvement of a skin aging symptom.

Claims (3)

A cell activator characterized by comprising an extract of Snagoke ( Racomitium canescens ) as an active ingredient. A whitening agent characterized by containing an extract of Snagoke ( Racomitium canescens ) as an active ingredient. A skin external preparation for improving aging prevention, characterized by comprising an extract of Snagoke ( Racomitium canescens ) as an active ingredient.