KR20160139632A

KR20160139632A - Allium monanthum extracts of biological activity

Info

Publication number: KR20160139632A
Application number: KR1020150074854A
Authority: KR
Inventors: 주동조; 배은영; 이준원
Original assignee: 배재대학교 산학협력단; 주동조
Priority date: 2015-05-28
Filing date: 2015-05-28
Publication date: 2016-12-07

Abstract

The present invention relates to an anti-inflammatory or anticancer composition comprising an Allium monanthum extract as an active ingredient and, more specifically, to an antioxidant or anticancer composition comprising an Allium monanthum extract, which is extracted by using water or an organic solvent, and then fraction extracted with different polar solvents such as water, ethyl acetate, hexane, etc., as an active ingredient. The Allium monanthum extract of the present invention has excellent antioxidation activity and controls cell proliferation of cancer cells to prevent or treat cancer, thereby being used for manufacturing quasi drugs, cosmetics, and foods.

Description

Allium monanthum extracts of biological activity < RTI ID = 0.0 >

The present invention relates to a method for producing a soya extract having antioxidant and anticancer effects, and a cosmetic composition prepared by using the soya extract and the soya extract prepared by the above method.

Tumors are a collection of cells that exhibit autonomous overgrowth, distinguishing malignant tumors that may lead to death and benign tumors that are not. In particular, cancer is a genetic disorder caused by a mutation in genes such as oncogenes and tumor suppressor genes, and it is a cell-mediated disease. Cancer is widely classified into blood cancer and solid cancer, and it occurs in almost all parts of the body such as lung cancer, stomach cancer, breast cancer, oral cancer, liver cancer, uterine cancer, esophageal cancer and skin cancer.

Of the methods used to treat malignant tumors, chemotherapeutic agents other than surgery or radiation therapy are collectively referred to as anticancer drugs, and most of them exhibit anticancer activity by inhibiting the synthesis of nucleic acids. 5-fluorouracil (5-FU) is a water-soluble fluorinated pyrimidine analog, which has been widely used as an antitumor agent in the treatment of cancer, such as surgery, chemotherapy and radiation. (Kim et al., 2012). (Kanetaka et al., 2012), which is used alone or in combination with leucovorin (LV) for solid tumors such as liver cancer, stomach cancer, colon cancer, pancreatic cancer and breast cancer and has a plasma half- ), Bone marrow weakness, gastrointestinal reactions, leukopenia, and thrombocytopenia (Wettergren et al., 2012).

At present, many natural products are being used to improve health through immunity enhancement around the world. Drugs derived from natural products have been focused on active ingredients for the prevention and treatment of diseases. While traditional medicine books such as Dongbok-bong have mentioned the efficacy of natural products, the information in the literature is not associated with the current specific diseases or symptoms. In addition, it has not been revealed which of the ingredients of the natural product has a direct effect. Researches have been actively conducted to separate and extract substances exhibiting physiological activity contained in plants rather than directly using natural products known to have immunostimulating effects among natural products listed in Dongbokbo or Chinese Medicine Dictionary. At present, studies on tongoferol, phenolic substances, Maillard type browning products, amino acids, peptides, and the like are being conducted as physiologically active substances in natural products.

Allium monanthum Max. Is a perennial herbaceous plant belonging to the allium of Liliacase. It is a wild plant widely distributed in Northeast Asia including Korea, Japan, China and Mongolia. The general components contained in the algae were inorganic minerals such as calcium, phosphorus and iron in the outpost, 14 species in the anion, 15 species in the root, and 9 species in the leaves. In addition, fructose, glucose, sucrose and maltose have been reported in leaves and spermatozoa.

It is widely used not only for soothing food but also for medicinal purposes. The major medicinal effect is known to have an effect such as sterilization, hemostasis, blood, and nervous stability. The offensive odor of the garlic, onion (Allium sativum) I have a plant component, alliin, which is also called 3- (2-propenylsulfinyl) -L-alanine, S-allyl-L-cysteine sulfate. In addition, methyl alliin and scorodose have been reported as a component having antibacterial activity (Shanghai Science & Technology Press (1985) In "Encyclopedia of Chinese Drugs ", pp. 226, Shogakukan, Tokyo, Japan.).

The main researches on algae include taxonomic studies, cultivation studies, and bactericidal effects, and it has been reported that long - term treatment of algae affects serum lipid, sugar and protein content in rats. Among the components of soothing, many glycosylglyceride compounds have been studied in relation to anti-cancer and hyperlipidemia. However, there has been no report on the fractional extract of Alaskan which can maximize the anti-inflammatory activity and anticancer activity in the process of obtaining the extract from the algae so as to further enhance the industrial usefulness of algae.

In a study of Aoyama et al. (J. Korean Soc. Agric. Chem. Biotechnol., Vol. 43, No. 4, pp. 314-316 (2000) The polyphenols were firstly extracted with methanol, acidified with 5% hydrochloric acid / methanol solution, and then neutralized with neutralizing agent. Then, two kinds of flavonoid glycoside components were extracted from the extracts subjected to reduced pressure and concentration There have been reports on the extraction and analysis of the n - butanol fraction of the aloe vera which was confirmed to be able to extract the active ingredient through the extraction of silica gel column. However, there has been no report showing remarkable anti - cancer effect in addition to the antioxidative effect in the alum extract.

Korean Registered Patent No. 10-1211937 Korean Patent Publication No. 10-2010-0084209

"Discovery of Active Substances from Edible Plant Resources-Isolation of Flavonoid Glycosides from Allium monanthum Max.", J. Korean Soc. Agric. Chem. Biotechnol. Vol. 43, No. 4, pp. 314-316 (2000).

The deterioration of immune function is known to cause a variety of diseases including malignant tumors, breaking the homeostasis that maintains the balance of the living body due to deterioration of the processing function against the antigen from the outside. Especially, as the age increases, the severity of this phenomenon becomes more prominent and presents many problems in the coming aging society. It is well known that the ability of the immune response of a living body to become aged and deteriorate. It is also reported that the incidence of cancer in the liver and respiratory system is significantly increasing.

Cancer is a disease that has already progressed to disease and can not be cured by surgery. In such cases, cancer therapy or radiation therapy is tried, but its effect is not good and side effects are not satisfactory. Therefore, it is necessary to study the adjuvant which can be used together with anticancer drugs to reduce the side effects of anticancer drugs or to increase the anticancer effect, thereby improving the therapeutic effect and controlling the biological response.

Allium monanthum is a perennial plant belonging to Lillium. It has been widely used for its unique fragrance and taste. It has been widely used for sterilization, blood circulation, nervous stability, detoxification, And it has been proved that when the sole is administered to rats, the dietary efficiency, HDL-cholesterol and phospholipids of the rats are increased, and the serum total cholesterol and blood sugar are decreased, and thus it is effective in preventing adult diseases such as arteriosclerosis and diabetes mellitus have. However, there are few studies on the antioxidative and anticancer effects of the extracts of the soya extracts containing various herbal ingredients.

Accordingly, the present inventors intend to provide an extract having an antioxidant and anticancer effect, which comprises the extract of Solanum parvum as an active ingredient.

The compound of the present invention may be dried, preferably soaked in water to about 1 to 20 times, preferably about 3 to 15 times the dry weight of water, a C1 to C4 lower alcohol or a mixed solvent thereof, preferably Extraction with water, extraction with water, extraction with ultrasound, extraction with water or methanol at room temperature (20 to 100 ° C, preferably 20 to 30 ° C) extraction temperature for about 1 hour to 10 days, preferably about 20 hours to 30 hours, A first step of obtaining a crude extract by repeating extraction by 1 to 10 times, preferably 2 to 7 times, obtained by an extraction method such as reflux cooling extraction, preferably a reflux cooling extraction method, and then concentration under reduced pressure; The compound of the present invention can be obtained through a second-step preparation process in which the crude extract is suspended in water and then extracted with ethyl acetate and water, respectively.

More particularly, the present invention provides an extract having an anticancer activity activity of hepatocarcinoma, colon cancer, and prostate cancer against an extract containing the soya as an active ingredient.

1 is a graph showing cell viability (CCK-8 assay) of a water layer according to an embodiment of the present invention
2 is a graph showing cell viability (CCK-8 assay) of an ethyl acetate layer according to an embodiment of the present invention
3 is a graph showing the cell viability (CCK-8 assay) of the hexane layer according to the embodiment of the present invention
FIG. 4 is a graph showing the anti-cancer effect of the extract of the soya extract according to the embodiment of the present invention,

<Example 1> Preparation of the extract of Alumnae according to the present invention

2.4 kg of the dried soya used in the experiment was purchased from the budget and ground to a size of 30 meshes or less using a pulverizer. This was added to 15 l of 80% methanol solution and then subjected to primary extraction by reflux cooling at room temperature (24 ° C) for 24 hours. The residue was further extracted twice by the same method, and then added to the first extract to obtain the final extract. The above-obtained final extract was filtered and lyophilized to obtain a so-called crude extract (137 g). Extraction and separation of natural products in terms of production and industrialization related to known natural processes such as searching for unknown new substances in the early stages of natural products research and existing academic physiological activities. There is a separation method. Generally, the systematic extraction method used is extraction with petroleum ether, Et ₂ O, CHCl ₃ , MeOH, EtOH and H ₂ O in that order. The organic compounds contained in the plant material are then extracted from the nonpolar material in order of increasing polarity. Solvent extraction can be carried out using a non-polar and polar solvent. Non-polar solvents are hexane (n-hexane), polar solvents such as methyl alcohol and ethyl alcohol, have. Methanol (methyl alcohol) is the most commonly used solvent for extracting physiologically active substances from plants. It is not only easy to extract various components, but also is easy to separate and purify after extraction. Therefore, when it is desired to test the physiological activity against a certain sample, it is usually extracted with methanol and then proceed to the next step. The methanol extract is immediately concentrated and lyophilized to give a methanol-extracted dry powder.

The crude extract (137 g) obtained in the above manner was suspended in water (2 L) and put into a separating funnel to carry out the fractional extraction of three layers. Separation of the liquids was carried out by adding 2 l of hexane and ethyl acetate twice, followed by shaking, and the soluble portion isolated by each of the extracts was obtained. Each soluble portion was concentrated with a vacuum concentrator. The hexane soluble extract obtained by the above method was about 30 g, the ethyl acetate soluble extract was about 30 g, and the water soluble extract was about 80 g.

The extract having an antioxidative effect in the above manner is contained in an amount of 0.1 to 30% by weight based on the total weight, and the remaining 70% of the extract includes tannin, a polysaccharide, and a phenone compound.

<Example 2> Antioxidant test for the extract of alumnaeus according to the present invention

DPPH, ABTS, FRAP assay and total polyphenol compound content assay were used for the antioxidant assay to determine the antioxidant levels of the extracts of the extracts from the extracts of hexane, ethyl acetate and water obtained in Example 1 above.

<2-1> DPPH Radical Scavenging activity analysis

Although direct measurement of the amount of active oxygen produced in the body or evaluation of the ability to absorb oxygen free radicals is the most ideal way to measure the reactive oxygen scavenging ability, Since much time is required, there is a limitation in measuring the activity of many samples. In this respect, the measurement of DPPH (1,1-diphenyl-picrylhydrazyl) radical scavenging activity is a fast, simple and economical method for measuring antioxidant activity. The DPPH radical scavenging activity method is widely used in food and other fields as a method of evaluating free radical scavenging ability and hydrogen donating ability of a sample. Most radicals are very unstable due to their reactivity, but the DPPH radical is a dark purple compound with a strong absorption at 517 nm as a stable free radical. However, when hydrogen is supplied from an antioxidant capable of scavenging free radicals, it changes to yellow when it becomes DPPHn, and the absorbance decreases at 517 nm. Therefore, the antioxidative activity of the sample can be simply measured using this principle. Antioxidant activity was measured by the Blois method (Blois, 1958), which measures the radical scavenging effect of DPPH.

1 mL of each extract was added to 1 mL of each concentration, and 0.2 mM DPPH solution (1 mL) was added. The mixture was shaken with a vortex mixer for 10 seconds, left in a dark room for 30 minutes, and then absorbed at 517 nm. BHA, a conventional antioxidant, was used as a control.

(E: experimental group, B: blank, C: control group)

sample DPPH (%) Dala Water extract 38.3 ± 0.7 Ethyl acetate extract 52.3 ± 0.4 Hexane extract 37.6 ± 0.2 BHA 90.8 ± 0.5

As shown in Table 1, the free radical scavenging rate of the extracts of the algae was not higher than that of the conventional antioxidant BHA, but it was about 14% higher than that of ethanol extracts As a result, it was confirmed that the extract of ethyl acetate extract showed a relatively high antioxidant component among the extract components of soya extract.

<2-2> ABTS Radical Scavenging activity analysis

Total antioxidant activity was measured by ABTS radical scavenging method. That is, 7 mM ABTS and 140 mM potassium persulfate solution were mixed and allowed to stand in the dark for one day to form ABTS · +. This solution was then diluted with ethanol to an absorbance value of 0.7 +/- 0.02 at 734 nm. To 1 mL of the diluted ABTS · + solution, 50 μL of the diluted extract at a concentration of 20 mg / mL in methanol was added and the change in absorbance was measured after 3 minutes.

Table 2 shows the values of ABTS radical scavenging activity (ABTS IC ₅₀ ) of 50%, and ABTS detection results were compared with vitamin C, which is a representative antioxidant.

sample ABTS (IC ₅₀ mg / mL) Dala Water extract 2.8 ± 0.1 Ethyl acetate extract 0.50 + - 0.1 Hexane extract 5.54 + 0.1 Vitamin C 0.05 ± 0.1

In the ABTS radical scavenging ability, when extracting the active ingredient with the non-polar solvent hexane, the antioxidative properties of the extracts of polar water were about 2 times higher than those of the water extract of the polarity, which is about 100 times higher than that of the control sample vitamin C .

<2-3> FRAP Scavenging activity analysis

The reagents used in the FRAP assay were 10 mM TPTZ (2,4,6-tripyridyl-S-triazine) dissolved in 0.3 M acetate buffer (pH 3.6) and 40 mM hydrochloric acid (HCl) , And 20 mM ferric chloride (FeCl ₃ ₎ solution were used. A pre-prepared sodium acetate buffer solution, a TPTZ solution and a ferric chloride solution (FeCl ₃ ) were mixed at a ratio of 10: 1: 1 (volume ratio, v / v / v) To prepare FRAP samples. Using a methanol vortex mixer, mix 900 μl of the FRAP sample with 30 μl of the sample at a concentration of 20 mg / ml and 90 μl of distilled water. After incubation at 37 ° C for 10 minutes, the absorbance was measured at 593 nm. The results are shown in Table 3.

sample FRAP (mM) Dala Water extract 125.8 ± 5.3 Ethyl acetate extract 458.8 ± 5.9 Hexane extract 319.1 ± 2.9 Vitamin C 68,137.5 ± 6.2

The antioxidant activity of the extracts of the soya using ethyl acetate was slightly different from the antioxidant activity of the extracts of hexane by the FRAP test method. This difference was significantly different from that of vitamin C, which is the highest control value .

The total polyphenolic compound content (TPC) was analyzed using the Folin-Ciocalteu method. 0.2 mL of Folin-Ciocalteu reagent (Sigma Chemical Co., St. Louis, Mo., USA) was added to 0.1 mL of each of the extracts and fractions diluted with methanol, and the mixture was maintained at 23 ° C for 1 minute. Then, 3 mL of 5% sodium carbonate (Na ₂ CO ₃ ) was added and the mixture was allowed to stand at 23 ° C for 1 hour. Absorbance was measured at 725 nm using a spectrophotometer (UV-1800, Shimadzu Co., Kyoto, Japan). The calibration curves were prepared using gallic acid (Sigma Chemical Co., St. Louis, Mo., USA) containing tannin components, and the content was calculated and shown in Table 4.

sample TPC (mM) Dala Water extract 92.5 ± 2.2 Ethyl acetate extract 69.7 ± 1.6 Hexane extract 26.5 ± 0.9

The water extract of dried soya has higher polyphenol content than the ethyl acetate or hexane extract. This indicates that the polyphenol component is better extracted from polar water, and the extract content of the polyphenol component is three times or more that of the weakly polar hexane extract.

The extracts of DPPH, ABTS, FRAP, and TPC, which were used as the antioxidant active ingredients of the soya extracts, showed the highest antioxidative activity in DPPH and FRAP assay The results are shown in Fig.

<Example 3> Analysis of anticancer activity according to the present invention

<3-1> Cancer cell line culture

In order to investigate the anticancer activity of the extract of the soya extract according to the present invention, the inhibitory effect on cancer cell proliferation of four cancer cells was analyzed.

The cancer cell lines used in the present invention were firstly transfected with HaCaT (Kera tinocyte) cells, human hepatocellular carcinoma cell line HePG2 (Hepatocarcinoma), human colon cancer cell line HCT 116 (Colon carcinoma) and human prostate cancer cell line CP3 (Prostatic carcinoma) Respectively.

HaCaT cells, HCT 116 cells, and PC3 cells were purchased from Korean Cell Line Bank (KCLB). HepG2 (Hepatocarcinoma) was purchased from the American Type Culture Collection (ATCC).

Each dispensed cell line was cultured in DMEM (Invitrogen, Carlsbad. CA) medium supplemented with 10% fetal bovine serum (FBS) and 100 units / mL penicillin and 10 ug / ml streptomycin And cultured in a 37 ° C incubator containing 5% CO ₂ . After the cells were cultured for 2 weeks or more, the cell density was saturated to 70 ~ 80%. After the cells were incubated for 5 min with 0.05% trypsin-EDTA solution, the cells were subcultured.

<3-2> CCK -8 assay Using Cancer cell line Growth inhibition effect measurement

Analysis of dehydrogenase as a catalyst for redox reaction necessary for respiration reaction of organic compounds among methods for measuring cell viability for measuring the inhibitory effect of an extract on cancer cell growth by using microorganism activity measurement CCK-8 (Dojindo, Kumamoto, Japan), a kit for measuring cell numbers, was performed. CCK-8 was prepared by using tetrazolium salt (WST-8 *), which is near colorless, and samples were fractionated into HaCaT, HepG2, HCT116 and PC3 cells cultured on 96-well plates for 48 hours, 250 ㎍ / ml, cultured for 48 hours, CCK-8 was added to each well, and further incubated for 1 hour. Cell proliferation was expressed as a percentage of the absorbance of the control in the absorbance of the sample.

The results of inhibiting the cancer cell inhibitory effect as measured by the above method are shown in FIGS. 1 to 3, which are classified into water extract, ethyl acetate extract and hexane extract.

As a result of addition of each fraction extract, it was confirmed that as the concentration of the sample increases, the proliferation inhibition effect increases in all cancer cell lines.

When the water - soluble extract of the extracts from the water extracts was used at 250 ㎍ / ml, the inhibitory effect of HCT 116, a colon cancer cell line, was remarkable.

Compared with the cell inhibitory effect by the water soluble extract, the ethyl acetate soluble extract of FIG. 2 showed a 25% reduction in the cancer cell survival rate when treated with 100 ㎍ / ml or more in each cancer cell line, and 250 ㎍ / ml Treatment resulted in remarkable cancer cell survival lowering effect in all cancer cell lines. In particular, HCT116, a colon cancer cell line, showed a 50% decrease in cell viability, thus showing a remarkable anticancer effect of the extract of Solae extract of ethyl acetate.

Compared with the treatment effect of water and ethyl acetate soluble extracts of soleae, the extracts of Solanella hexane extract showed a remarkable effect on the decrease of survival rate of all cancer cells when treated with 100 ㎍ / ml or more. Especially, 116 showed a cell survival rate of less than 30% at 100 ㎍ / ml or more, indicating high anticancer effect. The results of treatment with the extracts of hexane extracts of 250 μg / ml or more showed a low cell survival rate of less than 50% in HepG2, HCT116 and PC3. Especially, treatment with HCT116, a prostate cancer cell line, and PC3, And 25%, respectively. Thus, the present invention is effective in inhibiting the growth of these cancer cells.

As a result of the above analysis, each of the extracts was treated with HaCaT, HepG2, HCT116, and PC3 cell lines cultured at 250 / / ml in order to exhibit anticancer effects of the fractions of the algae extracts. The cell culture DMEM medium (Dulbecco's Modified Eagle's Medium) As shown in Fig. From FIG. 4, it can be seen that, in the case of the extract obtained by extracting hexane from algae, HCT 116, which is a colon cancer cell line, and PC3 which is a prostate cancer cell line, cancer cell clearance is more remarkable than that of the control group, The difference in cancer cell clearance was found to be significantly different from that of the control group.

The anticancer effects of water, ethyl acetate, and hexane extracts obtained by extracting 80% methanol from the soya extracts were analyzed, and the anticancer effect of the extracts was confirmed. .

Example 4 Preparation of a Cosmetic Composition Using the Solanum Extract According to the Present Invention

The cosmetic composition of the present invention can be prepared by adding various known ingredients and the like to the skin by using a method known in the art to prepare a cosmetic composition containing nutritional lotion, softening longevity, nutritional cream, massage cream, essence, eye cream, cleansing water, cleansing cream, , A spray, and a powder.

Specifically, when the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel or a pack, known components include animal fibers, plant fibers, waxes, paraffin, starch, tragacanth, cellulosic derivatives, polyethylene glycol, , Silica, talc or zinc oxide may be used.

When the formulation of the cosmetic composition of the present invention is a powder, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or the like may be used.

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solvent or an emulsifier is used, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or non-acidic esters of sorbitan, and the like.

When the formulation of the cosmetic composition of the present invention is a suspension, it may be diluted with water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Stearylcellulose, aluminum metahydroxide, bentonite, agar or tragacanth, and the like can be used.

When the formulation of the cosmetic composition of the present invention is a detergent containing a surfactant, it is preferable to use a surfactant-containing detergent such as aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil or ethoxylated glycerol fatty acid ester, and the like can be used.

In addition, the cosmetic composition of the present invention can be prepared by any of the commonly known formulations such as stabilizers, solubilizers, vitamins, pigments, perfumes, and the like, And can be selected accordingly.

<4-1> Production of Nourishing Lotion Using Solanum Extract

As an example of the formulation, a composition of the nutritional lotion formulation among the cosmetics containing the extract of the soymilk of Example 1 was prepared by the composition of the following Table 5 and the following manufacturing method. 10, 11, 13, and 16 of Table 5 were heated to between 80 and 85 ° C with mixing and stirring, and then charged into the manufacturing section. Then, an emulsifier was operated and 2, 3, 4, 5, 6, 7, 8, To 85 ° C and then emulsified. After the emulsification was completed, the mixture was thermally cooled to 50 ° C. with stirring using an agitator, and then the mixture was cooled to 45 ° C. After the mixture was cooled to 45 ° C., the mixture was stirred at 35 ° C. and cooled to 25 ° C. A cosmetic product was obtained.

ingredient Raw material Content (% by weight) One The soothing extract (Example 1) 20.0 2 Wax 1.0 3 Polysorbate 60 1.5 4 Sorbitan sesquioleate 0.5 5 Liquid paraffin 10.0 6 Sorbitan stearate 1.0 7 Pro-type glycerin monostearate 0.5 8 Stearic acid 1.5 9 Glyceryl stearate / FG-400 stearate 1.0 10 Propylene glycol 3.0 11 Carboxy polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16 Purified water Balance 17 water -

<4-2> Production of softened longevity using the extract

As an example of the formulation, the softening longevity was prepared by the composition of the following Table 6 containing the soothing extract of Example 1 and the following production method.

ingredient Raw material Content (% by weight) One Soothing extract 5.0 2 ethanol 5.0 3 Betaine 3.0 4 Diel panthenol 3.0 5 Sebom-A 1.0 6 Arbutin paste 2.0 7 Anemycin 2.0 8 Eco-sword 0.03 9 glycerin 4.0 10 Collagen 1.0 11 Plant extract 5.0 12 antiseptic a very small amount 13 Purified water Balance

<4-3> Manufacture of Nourishing Cream by Using a Solanum Extract

As an example of the formulation, the nutritional cream was prepared by the composition of the following Table 7 containing the soothing extract of Example 1 and the following manufacturing method.

ingredient Raw material Content (% by weight) One The soothing extract (Example 1) 10.0 2 Stearic acid 2.0 3 Cetyl alcohol 2.0 4 Glyceryl monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Sorbitan sesquioleate 0.5 7 Glyceryl monostearate
/ Glyceryl stearate
/ Polyoxyethylene stearate 1.0 8 Wax 1.0 9 Liquid paraffin 4.0 10 Squalane 4.0 11 Caprylic / Capric Triglyceride 4.0 12 Carruboxyvinyl polymer 0.3 13 Butylene glycol 5.0 14 glycerin 3.0 15 Triethanolamine 0.5 16 Purified water Balance

<4-4> Manufacture of massage cream using algae extract

As an example of the formulation, the nutritional cream was prepared by the composition of the following Table 8 containing the soothing extract of Example 1 and the following manufacturing method.

ingredient Raw material Content (% by weight) One Soothing extract 5.0 2 Wax 10.0 3 Polasolbait 1.5 4 Sorbitan sesquioleate 0.8 5 Liquid paraffin 40.0 6 Squalane 5.0 7 Caprylic / capric triglyceride 4.0 8 glycerin 5.0 9 Butylene glycol 3.0 10 Propylene glycol 3.0 11 Triethanolamine 0.2 12 antiseptic a very small amount 13 Pigment a very small amount 14 Spices a very small amount 15 Purified water Balance

<Example 5> Preparation of food using the extract of Alumnae according to the present invention

&Lt; 5-1 > Food Formulation Example 1: Soft capsule

As an example of the formulation, the health functional food of soft capsule was prepared by the following composition and the following production method containing the soothing extract of Example 1.

A soft capsule was prepared by mixing 400 mg of L-carnitine extract, 300 mg of L-carnitine, 180 mg of soybean oil, 2 mg of palm oil, 8 mg of vegetable hardening oil, 4 mg of yellow radish and 6 mg of lecithin per one capsule according to a conventional method.

&Lt; 5-2 > Food Formulation Example 2: Tablets

As an example of the formulation, the health functional food of tablets was prepared by the following composition and the following production method containing the extract of the soymilk of Example 1.

The extracts were mixed with 300 mg of extract, 300 mg of galactooligosaccharide, 150 mg of lactose, and 150 mg of maltose. The mixture was granulated using a fluidized bed drier, and 6 mg of sugar ester was added thereto.

&Lt; 5-3 > Food formulation example 3: Granule

As an example of the formulation, the health functional food of the granule was prepared by the following composition and the following production method containing the soothing extract of Example 1.

250 mg of anhydrous crystalline glucose and 450 mg of starch were mixed and granulated into granules using a fluidized bed granulator and filled in a capsule.

&Lt; 5-4 > Food formulation example 4:

As an example of the formulation, the health functional food of the drink was prepared by the following composition and the following production method containing the soot extract of Example 1.

300 mg of the extract, 300 mg of glucose, 10 g of citric acid, 0.6 g of citric acid, and 25 g of liquid oligosaccharide are mixed, and 300 ml of purified water is added. And then sterilized at 130 DEG C for 4 to 5 seconds to prepare a beverage.

&Lt; 5-5 > Food formulation example 5: Caramel formulation

As an example of the formulation, the health functional food made of caramel was prepared by the following composition and the following production method containing the extract of the soymilk of Example 1.

10 g of the soya extract, 1.8 g of corn syrup, 1.0 g of skim milk, 1.0 g of soybean lecithin, 0.6 g of butter, 0.4 g of vegetable cured oil, 1.4 g of sugar, 0.58 g of margarine and 20 mg of salt were mixed and caramelized .

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive.

Claims

Containing extracts as an active ingredient,
Crushing the dried soot;
Mixing pulverized soap and solvent; The mixed solution was adjusted in temperature and time
extraction;
The extracted solution was concentrated under reduced pressure; A soya extract prepared by freeze-drying
;
Suspending the sooty crude extract in water;
And a step of fractionating the above-mentioned suspended solu- tion extract with a solvent
&Lt; / RTI >

The method according to claim 1, wherein, in the process of preparing the soya extract, the ratio of the dry weight of the soya to the water is mixed with water at a ratio of 1: 3 at a weight ratio of 1: 3 to water, A method for producing a composition comprising an extract as an active ingredient.

The process according to claim 1, wherein the weight ratio of alcohol to water is in the range of from 1:15 to 4: 5 in the course of preparing the mixed solution of soap and solvent. A method for producing a composition comprising an extract as an active ingredient.

The method for preparing a composition as claimed in claim 1, which comprises extracting a soymilk extract comprising a step of extracting a solution of soya bean and a solvent at an extraction temperature between 20 ° C and 100 ° C.

The method for preparing a composition according to claim 1, which comprises extracting a solution of soya bean and a solvent mixture for a period of time ranging from 1 hour to 10 days.

The method for producing a composition according to claim 1, wherein the composition comprises a soya extract prepared by a method of obtaining a soymilk extract composed of one of a soap and a solvent mixed solution, such as cold extraction, hot water extraction, ultrasonic extraction and reflux cooling extraction.

The method for producing a composition according to claim 1, which comprises extracting a mixture solution of soya bean and a solvent by extracting with one to ten times of extraction.

The method for preparing a composition according to claim 1, which comprises extracting a soxworm extract suspended in water with a solvent selected from the group consisting of ethyl acetate, hexane and water.

The cosmetic composition according to any one of claims 1 to 3, wherein the cosmetic composition has an anti-inflammatory improving effect containing 0.1 to 30% by weight (when the total amount of the composition is 100% by weight).

The cosmetic composition according to claim 9, wherein the cosmetic composition comprises the formulation of softening agent, nutritional lotion, nutritional cream, massage cream, essence, eye cream, cleansing water, cleansing cream, cleansing foam, pack, spray, powder .

Wherein the composition has a preventive effect on liver cancer, colon cancer and prostate cancer containing 0.1 to 30% by weight (when the total amount of the whole composition is 100% by weight).

12. The food composition according to claim 11, wherein the food composition containing the extract has a soft capsule, tablet, granule, and drink formulations.