JP5294847B2 - Moisturizer, whitening agent and slimming agent - Google Patents

Description

  The present invention relates to a humectant, a whitening agent, and a slimming agent. More specifically, the present invention relates to a moisturizing agent, a whitening agent and a slimming agent, which contain an extract of the family Iwadendae as an active ingredient.

  Factors of skin symptoms such as fine wrinkles, dry skin, rough skin, spots and dullness include drying in a low humidity environment such as an air conditioner or a highly airtight house, a decrease in skin barrier function, melanin production and pigmentation due to ultraviolet rays. In order to prevent and improve such skin symptoms, various active ingredients have been conventionally studied.

  In the moisturizing agent, as a moisturizing agent having excellent moisturizing effect and safety, an extract of a plant belonging to the genus Compositae (Wedelia) (see Japanese Patent Application Laid-Open No. 2002-20262) and a family of Brassica (Cruciferas) Extracts of plants belonging to the genus Lepidium (see JP 2005-281271 A) are known.

  As a whitening agent or a melanin production inhibitor, water and / or organic solvent extract of white crane reishi (see Japanese Patent Application Laid-Open No. 2003-89630), extract of Korean dung beetle, pine bush, vinan quail (Japanese Patent Application Laid-Open No. 2004-244326) Reference) and kale extract (see Japanese Patent Application Laid-Open No. 2004-91396) are known.

  Apart from these, in recent years, various diseases such as obesity and hyperlipidemia caused by excessive food intake, lack of exercise, stress and the like have become serious social problems. In order to prevent and ameliorate such obesity and diseases, various methods have been studied in the past, such as dietary restriction and exercise methods, intake of dietary fiber, utilization of a lipolysis promoter, and the like. However, these are mainly methods for reducing fat already accumulated in the body, and are considered insufficient as a fundamental improvement.

  The method of suppressing the accumulation of fat in the living body directly suppresses the accumulation of fat in the body, so it is excellent in fundamental improvement of obesity and diseases, and is effective as a daily preventive method. is there. Examples of components having a fat accumulation-inhibiting action for inhibiting fat accumulation in the living body include phospholipids derived from mammalian milk (see JP-A No. 2001-275614), enzymatic degradation products of brown algae (JP-A No. 7). No. 278,005) is known.

  Naturally derived components are known to have various pharmacological and cosmetic effects, and so far many plants and fungi have been widely applied to fields such as external preparations for skin and foods and drinks. However, there are many naturally derived ingredients whose effects are not yet known, and the development of excellent active ingredients has been expected.

  The present inventors have made various studies in order to find various safe and naturally derived substances that have excellent pharmacological and cosmetic effects and can be widely applied to fields such as external preparations for skin and foods and drinks. Went. As a result, the present inventors have found that excellent moisturizing action, whitening action, and slimming action are found in the family Aridaceae, and have further studied and completed the present invention.

That is, this invention relates to the moisturizer which uses as an active ingredient the 1 type, or 2 or more types of plant chosen from the Iwadenidae plant, or its extract.
Another aspect of the present invention relates to a whitening agent comprising one or more plants selected from the family Aridaceae or an extract thereof as an active ingredient.
Another aspect of the present invention relates to a slimming agent comprising as an active ingredient one or more kinds of plants selected from the family Iridaceae or extracts thereof.
Another aspect of the present invention relates to a composition containing any one or more of a moisturizer, a whitening agent, and a slimming agent according to the present invention.
Another aspect of the present invention relates to a composition for external use comprising one or more plants selected from the family Iridaceae or an extract thereof.
Another aspect of the present invention relates to an oral composition containing one or more plants selected from the family Iridaceae or an extract thereof.
Yet another aspect of the present invention relates to an extract of one or more plants selected from the family Iwadendae.

ADVANTAGE OF THE INVENTION According to this invention, the moisturizing agent, whitening agent, and slimming agent which have the outstanding moisturizing, whitening, and slimming effect can be provided by mix | blending the Iwadendaceae plant or its extract.
These humectants, whitening agents, and slimming agents can be used in various fields such as cosmetics, external preparations for skin such as external medicines, and foods. In other words, by blending these, various external compositions or oral which can prevent and improve various skin symptoms such as fine lines, dry skin, rough skin, spots, dullness, and also have an excellent effect on slimming action. A composition can be provided.

Woodsiaceae plants, which are plants used in the present invention, are evergreen or summer-green terrestrial fern plants that are widely distributed from the tropics to the temperate and cold zones of the world, and some grow on rocks. On behalf of the name in the famous edible wild plants ostrich fern genera ostrich fern (grass Cycad) (Matteuccia struthiopteris) of "Kogomi", Kouyawarabi genus Kouyawarabi (bracken Takano) (Onoclea sensibilis L.var. Interrupta Maxim .), Kouyawarabi genus matteuccia orientalis (dog Wild Goose feet) (Onoclea orientalis (Hook.) Hook.), Iwadenda genus Iwadenda (Woodsia polystichoides Eaton), Iwadenda genus Fukuroshida (bag fern) (Woodsia manchuriensis Hook.), Kinmouwarabi genus Kinmouwarabi (gold hair bracken) (Hypodematium crenatumssp. fauriei), Ushhimewarabi (Utsuhimewarabi) ) (Acystopteris japonica), Usagishida genus Usagishida (rabbit fern) (Gymnocarpium dryopteris), Nayoshida genus Nayoshida (Cystopteris fragilis), Meshida genus athyrium niponicum (dog bracken) (Athyrium niponicum), Meshida genus Hosobainuwarabi (acinar dog bracken) (Athyrium iseanum ).
These plants can be used alone or in combination of two or more.

When using the Iwadenidae plant, for example, its young shoots, stems, leaves, roots and the like can be crushed and used as they are, but it is preferable to use extracts from those plants.
For extraction, any part such as young shoots, stems, leaves, roots, etc. of the family Iwadendae may be used, but it is preferable to use young shoots from the viewpoint of effectiveness. You may extract using 2 or more types of different site | parts. Further, two or more extracts extracted with different solvents may be mixed.
In the extraction, the plant may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization.
The extraction can be performed by immersing in an arbitrary extraction solvent for a predetermined time or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase extraction efficiency, stirring or homogenization in an extraction solvent may be performed. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but is suitably about 1 hour to 14 days.

  As an extraction solvent, in addition to water, lower alcohols such as methanol, ethanol, propanol and isopropanol; polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin; ethers such as ethyl ether and propyl ether Solvents such as esters such as butyl acetate and ethyl acetate; ketones such as acetone and ethyl methyl ketone can be used, and one or more of these are selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  Extracts of the above-mentioned solvents of Iwadendaceae plants can be used as they are, but they may be left to stand for a certain period of time and matured, or the concentrated and dried solids are dissolved again in water or a polar solvent. Can also be used. It may be used after performing purification treatment such as decolorization, deodorization, desalting, etc. or fractionation treatment by column chromatography or the like within a range not impairing these physiological effects. The said extract of the Iwadendaceae plant, its processed material, and the fractionation thing can also be freeze-dried after each processing and fractionation, and it can also be melt | dissolved and used for a solvent at the time of use.

The Iwadendae plant has excellent moisturizing action, whitening action, and slimming action, and can be preferably used as a moisturizing agent, whitening agent, and slimming agent.
These moisturizers, whitening agents, and slimming agents are not limited at all in terms of their forms and the presence or absence of other components, as long as they include the Iwadenidae plant or its extract as an active ingredient. As for the form, any form such as liquid, paste, gel, solid, powder, etc. can be selected depending on the application, etc., and the vehicle (excipient), solvent and other necessary for making the form Common additives (antioxidants, colorants, etc.) can optionally be included.

A moisturizing agent comprising an Iwadendaceae plant or an extract thereof as an active ingredient exhibits an excellent moisturizing effect on the skin, hair and the like, and is particularly excellent in the moisturizing effect on the skin. Specifically, it is evaluated by measuring the amount of moisture and the amount of moisture transpiration, and an excellent moisturizing effect can be confirmed.
A whitening agent comprising an Iwadendaceae plant or an extract thereof as an active ingredient exhibits an excellent whitening effect, specifically, an excellent tyrosinase activity inhibitory action and a melanin production inhibitory action.
A slimming agent comprising an antaceae plant or an extract thereof as an active ingredient exhibits an excellent slimming action, and in particular, an excellent effect on neutral fat accumulation inhibiting action.

These moisturizers, whitening agents and slimming agents can be applied not only to the skin, but also to the hair and the like, and can be taken orally, and applied to various compositions such as external compositions and oral compositions. Is possible.
Here, the composition for external use is not limited to any category such as cosmetics, external preparations for skin, quasi-drugs, and external medicines, but means all compositions for external use on skin or hair. doing. The oral composition also means all compositions that are taken orally regardless of the type of pharmaceuticals, foods, beverages and the like.
Specifically, various types of cosmetics such as emulsions, creams, lotions, packs, cosmetic liquids, cleaning agents, makeup cosmetics, etc .; quasi-drugs in various forms such as liquids, ointments, aerosols, patching agents, etc. Examples include topical pharmaceuticals; general foods including beverages; health foods or functional foods such as tablets, capsules, granules, powders, etc .; oral drugs such as tablets, capsules, granules, powders, syrups, extracts, etc. it can.

  For example, prevention and improvement of skin symptoms such as fine wrinkles, dry skin, rough skin, spots, dullness, etc. by blending a plant of the family Iwadendidae or its extract into an external preparation for skin including cosmetics, topical drugs, quasi drugs Can be obtained, and can also be used as a moisturizing skin external preparation or a whitening skin external preparation. Furthermore, the lobster family plant or the extract thereof can be used for food and drink, health food (supplement), functional food and the like for the purpose of beauty such as whitening and slimming, health maintenance or nutritional supplementation.

Compositions such as externally used compositions or oral compositions include, in addition to Iwadendaceae plants or extracts thereof, normal skin cosmetics, hair cosmetics, quasi-drugs, pharmaceuticals, depending on their use and necessity. Any ingredients used in such formulations are included. Such components include water, oils (oil-based ingredients), moisturizers, powders, pigments, emulsifiers, solubilizers, gelling agents, detergents, UV absorbers, anti-inflammatory agents, thickeners, surface activity. Agents, chelating agents, drugs (medicinal components), fragrances, resins, antibacterial and antifungal agents, pH adjusters, antioxidants, alcohols and the like. In addition, other moisturizers, whitening agents, slimming agents, antioxidants, cell activators, or plants other than the Iwadendaceae plants or extracts thereof can be used as long as the effects of the present invention are not impaired.
Also in the case of food and drink, there is no particular limitation in combination with various components used in food.

  The compounding amount of the teraceae plant or the extract thereof in the composition for external use or oral composition can be adjusted depending on the kind of composition and the purpose of use. From 0.0001 to 10% by mass in terms of solid content is preferable with respect to the total amount, more preferably 0.001 to 5% by mass, still more preferably 0.01 to 5% by mass, and still more preferably. It is 0.1-5 mass%.

  The dosage form of the composition for external use is arbitrary, and can be provided as, for example, a solubilizing system such as lotion, a dispersion system, or an emulsifying system such as cream or emulsion. Furthermore, it can also be provided in various dosage forms such as aerosol form, ointment, powder, granule filled with propellant.

  In the following, the extraction method of the extract of the family Iwadendaceae, the test method for evaluating each action, the formulation example as a skin external preparation and food and drink, will be described in more detail, but the technical scope of the present invention is thereby It is not limited at all.

  First, the preparation method of a plant extract is illustrated. By using Extraction Method 1, Extraction Method 2 and Extraction Method 3 shown below, Iwadenidae plant extracts used for moisturizing agents, whitening agents and slimming agents were prepared. A list of each extract and the example number using it is shown in Table 1.

<Extraction method 1>
The young shoots and young shoot stem parts of each of the wood sects ( Woodsiaceae ) shown in Table 1 are dried and pulverized, added with 40% amount of 50% aqueous solution of ethanol and extracted for 2 hours while stirring at room temperature. The insoluble matter was removed by filtration. After concentration under reduced pressure, freeze-drying was carried out to obtain an ethanol extract of the family Aridaceae.

<Extraction method 2>
Table Each Iwadenda family shown in 1 (Woodsiaceae) drying the bud and / or plant stems and ground, with respect to the sample 5g adding purified water 100g weight (20 times) the autoclave (120 °, 20min) and Extracted using. The solution was subjected to suction filtration while maintaining a high temperature, and then freeze-dried to obtain a hot water extract of the family Aridaceae.

<Extraction method 3>
The whole plant of each plant ( Woodsiaceae ) shown in Table 1 was put into a supercritical extraction apparatus, and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under a pressure of 15 MPa. The extract was recovered to obtain a supercritical extract of the family Aridaceae.

The moisturizing effect of the extracts of the family Iwadendae was evaluated.
<Evaluation of moisturizing effect by measuring moisture content>
For the extracts of Examples 1, 2, 3, 4, 7, 9, 11, 13, 15, and 17 in Table 1, the moisture content was measured as a test for evaluating the moisturizing action. First, the sample was applied to a membrane filter (Millipore Type JH, 0.45 μm) (15 mg / cm ² ) and mounted on a vial (capacity 13 mL, opening diameter 12 mm) containing 10 mL of distilled water. This was allowed to stand at room temperature (20 ± 3 ° C., relative humidity 40 ± 3%), and the moisture content of the sample coating film after 1 hour was measured. An optical fiber type near infrared (NIR) moisture meter IR-MF200 (manufactured by Chino Co., Ltd.) was used for the measurement of the moisture content. For comparison, Comparative Example 1: Purified water, Comparative Example 2: 50% by mass 1,3-butylene glycol, Comparative Example 3: 50% by mass glycerin were used.

<Evaluation of moisturizing effect by measuring moisture transpiration>
For the extracts of Examples 1, 2, 3, 4, 7, 9, 11, 13, 15, and 17 in Table 1, the amount of moisture transpiration was measured as another test for evaluating the moisturizing action. For measuring the amount of moisture transpiration, 2 mL of a 1% by weight aqueous solution of a moisturizing component was placed in a vial bottle with an inner diameter of 15 mm and a capacity of 13 mL, and at room temperature (20 ± 3 ° C., relative humidity 40 ± 3) in an open system without a lid. %) And the mass change after 24 hours was measured. The amount of water transpiration was evaluated with the average value of three samples. Regarding the measurement of the amount of water transpiration, the extraction method 1 was affected by ethanol as an extraction solvent, and therefore was used after drying under reduced pressure to remove ethanol. Similarly, Comparative Example 1: Purified Water, Comparative Example 2: 50% by mass 1,3-butylene glycol, and Comparative Example 3: 50% by mass glycerin were used for comparison.

The moisturizing effect was evaluated according to the following criteria. The results are shown in Table 2.
<Criteria based on moisture content>
A: Water content is 15% by mass or more B: Water content is 10% by mass to less than 15% by mass C: Water content is 5% by mass to less than 10% by mass D: Water content is less than 5% by mass Evaluation criteria>
A: Moisture transpiration is less than 0.12 g B: Moisture transpiration is from 0.12 g to less than 0.13 g D: Moisture transpiration is 0.13 g or more

  As is apparent from Table 2, it was confirmed that the extract of the family Lidadaceae of Examples has an excellent moisturizing effect as compared with purified water and a conventional moisturizing agent.

  Next, the whitening action of the extracts of Examples 1, 4, 7, 9, 11, 13, 15, and 17 in Table 1 was evaluated among the extracts of the family Iwadendaceae. Specifically, two actions of human epidermal melanocyte tyrosinase activity inhibition and melanin production suppression ability using B16 melanoma cells were evaluated.

<Evaluation of human epidermal melanocyte tyrosinase activity inhibition>
Normal human epidermal melanocytes manufactured by Kurabo Industries Co., Ltd. were seeded in a 96-well microplate so that the number of cells was 3.0 × 10 ⁴ per well. As a seeding medium, Medium154S manufactured by Kurabo Industries Co., Ltd. was used. After 24 hours, the medium was replaced with a sample culture solution adjusted to the concentration of each extract shown in Table 3 with Medium 154S, and further cultured for 48 hours. Next, the cells were completely lysed by exchanging with 75 μl of 1% Triton-X-containing phosphate buffer, and 50 μl of this was used as a crude enzyme solution.
To the obtained crude enzyme solution, 50 μl of 0.05% L-dopa-containing phosphate buffer serving as a substrate was added and allowed to stand at 37 ° C. for 2 hours. The absorbance at 405 nm immediately after the addition of the substrate and at the end of the reaction was measured with a microplate reader, and the amount of dopamelanin produced was determined by introducing the difference between the two measured values into the following equation.
Amount of produced dopamelanin = {(405 nm value after reaction−405 nm value before reaction) −2.166} /5.238

  Next, the amount of protein in each well was measured using BCA Protein Assay Kit manufactured by PIERCE, and the amount of dopamelanin produced per unit protein amount was determined. Evaluation was carried out by obtaining a relative value when the amount of dopamelanin produced when no sample (extract) was added was defined as 100 as a control. The results are shown in Table 3.

<Evaluation of ability to suppress melanin production using B16 melanoma cells>
B16 mouse melanoma cells were seeded in a 90 mm dish so that there were 18000 cells per dish. As the seeding medium, Dulbecco's modified Eagle medium (DMEM) to which 5% by mass of fetal bovine serum (FBS) was added was used. After 24 hours, the culture medium was replaced with a sample culture solution adjusted to each extract concentration shown in Table 4 with 5% by mass FBS-added DMEM medium, and further cultured for 5 days. After completion of the culture, the cells were harvested by trypsin treatment, transferred to a 1.5 ml microtube, and centrifuged to obtain a cell precipitate.
About the obtained deposit, the blackening condition was visually determined according to the following criteria. In the evaluation, a 5% FBS-added DMEM medium containing 5% by mass FBS was used as a negative control, and a 5% FBS-added DMEM medium containing sodium lactate at a concentration of 50 mM was used as a positive control. These naked eye judgment results were used as indicators for sample judgment as judgment 5 (negative control) and judgment 1 (positive control). The naked eye evaluation was evaluated in five stages as shown below. The results are shown in Table 4.

<Criteria>
1: Not blackened at all 2: Slightly blackened 3: Blackened 4: Very blackened 5: Blackened significantly

  Simultaneously, Soluen-350 (Perkin Elmer Japan Co., Ltd.) was added to the precipitate and boiled, returned to room temperature, and the absorbance was measured with a spectrophotometer (U-3010) (500 nm).

  As is clear from Tables 3 and 4, it was confirmed that the extracts of the family Iwadendaceae of Examples have excellent tyrosinase activity inhibitory action and melanin production inhibitory action.

  Next, the slimming action of the extracts of Examples 1, 4, 7, 9, 11, 13, 15, and 17 in Table 1 was evaluated among the extracts of the family Iwadendidae. Specifically, the neutral fat accumulation inhibitory action using normal human preadipocytes was evaluated.

<Evaluation of neutral fat accumulation suppression using normal human preadipocytes>
Subcutaneous fat-derived normal human preadipocytes Cryo HPRAD-SQ (Sanko Junyaku Co., Ltd.) were seeded on a 96-well microplate so that the number was 1.0 × 10 ⁴ per well. PGM medium (containing 10% by mass FBS, 2 mM L-glutamine, 100 units / mL penicillin, 100 μg / mL Streptomycin) was used as the seeding medium. Immediately before the cells became confluent, the medium was replaced with a PGM-differentiation medium (containing 10 μg / mL insulin, 1 μM dexamethasone, 200 μM indomethacin, 500 μM Isobutyl-methylxanthine) supplemented with each concentration extract shown in Table 5. Differentiation induction was performed. After initiation of differentiation induction, the cells were cultured for 10 to 14 days until the control group matured and many lipid droplets accumulated in the cells. After the cells were collected, the cells were fixed using a 10% neutral buffered formaldehyde solution. After washing with PBS (−), 0.5 W / V% Oil Red O solution was added and incubated at 37 ° C. for 2 hours. After washing with PBS (−), methanol was added to extract the dye.
The absorbance at 550 nm of the obtained test solution was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the neutral fat accumulation inhibitory action was evaluated using the difference between the two measured values. The evaluation was carried out by obtaining a relative value when the amount of accumulated fat in the control group containing no extract was taken as 100. The results are shown in Table 5. In the table, ** indicates a significance probability of less than 1% (P <0.01) with ** with respect to the significance probability P value in the t-test.

  As is clear from Table 5, it was confirmed that the extracts of the family Iwadendae of Examples have an excellent neutral fat accumulation inhibitory action.

  Subsequently, prescription examples of external preparations for skin and foods and drinks containing the extract of the plant family Iidadidae according to the present invention will be shown.

[Formulation Example 1] Emulsion (1) Squalane 10.0 (mass%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 53.85
(11) Arginine (1% by weight aqueous solution) 20.0
(12) Extract of genus Kusotatsu [Example 2] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix uniformly.

[Prescription Example 2] Lotion (1) Ethanol 15.0 (mass%)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 83.18
(5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) An extract of the genus Kobayarabi [Example 5] 0.2
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.

[Prescription Example 3] Cream (1) Squalane 10.0 (mass%)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20 mass% aqueous solution) 15.0
(10) Purified water 40.7
(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Extract of Iwadanda sp. [Example 8] 1.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification is completed, add (11), start cooling, add (12) at 40 ° C., and mix uniformly.

[Formulation Example 4] Cosmetic liquid (1) Purified water 31.45 (mass%)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1% by weight aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by mass aqueous solution) 2.0
(16) Extract of the genus Astragalus [Example 10] 1.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C, add (16) and mix evenly.

[Formulation Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (mass%)
(2) Purified water 88.2
(3) Sodium hydroxide (10% by mass aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Rabbit fern rabbit fern extract [Example 14] 0.5
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Formulation Example 6] Face-wash foam (1) Stearic acid 16.0 (mass%)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 36.5
(8) Extract of Nayoshida genus Nayoshida [Example 16] 1.0
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.

[Prescription Example 7] Make-up base cream (1) Squalane 10.0 (mass%)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 69.6
(8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Extract of Mesida genus Warabi [Example 18] 1.0
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Prescription Example 8] Bath agent (1) Fragrance 0.3 (mass%)
(2) Extract of genus Kusotatsu [Example 1] 4.0
(3) Sodium bicarbonate 47.0
(4) Sodium sulfate 48.7
Production method: (1) to (4) are mixed uniformly.

[Prescription Example 9] Hair wax (1) Stearic acid 3.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 73.6
(11) Extract of the genus Kobayarabi [Example 6] 2.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Formulation Example 10] Granules containing Kusotatsu (kogomi) extract (1) Extract of Kusotatsu Genus Kusotetsu [Example 1] 5.0 (mass%)
(2) Tocopherol powder 2.0
(3) Starch 29.5
(4) Reduced maltose starch syrup 60.0
(5) Sucrose fatty acid ester 3.5
Production method: (1) to (5) are mixed uniformly.

[Prescription Example 11] Beverage (1) Extract of genus Kusotatsu [Example 2] 1.0 (mass%)
(2) Erythritol 1.0
(3) Citric acid 0.1
(4) Stevia 0.01
(5) Purified water 97.89
Production method: (1) to (5) are mixed uniformly.

[Prescription Example 12] Tablet (1) Extract of genus Kusotatsu [Example 1] 5.0 (parts by mass)
(2) Sucrose 7.0
(3) Eggshell calcium 39.0
(4) Reduced maltose starch syrup 44.0
(5) Sucrose fatty acid ester 5.0
Production method: After uniformly mixing (1) to (3), tableting is performed with a tableting machine to obtain tablets having a diameter of 10 mm and a mass of 300 mg.

Claims (4)

A moisturizing agent comprising as an active ingredient one or more plants selected from the genus Koyawarabi, Usuhimerabi, Rabbit fern, Nayoshida, and Mesida. A whitening agent comprising, as an active ingredient, one or more plants selected from the genus Koyawarabi, Usuhimerabi, Rabbit fern, Nayoshida, and Mesida. A slimming agent comprising, as an active ingredient, one or more plants selected from the genus Koyawarabi, Usuhimerabi, Rabbit fern, Nayoshida, and Mesida. A composition for external use comprising one or more plants selected from the genus Koyawarabi, Usuhimerabi, Rabbit fern, Nayoshida, and Mesida, or an extract thereof.