JP2005171098A - Antioxidant, skin cosmetic, and food and drink for beauty - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an antioxidant, an active oxygen scavenger and a radical scavenger, containing an active ingredient obtained from a natural product which works as a scavenger of active oxygen and/or radicals; and to provide skin cosmetics, and foods and drinks for beauty, by adding the same ingredient thereto. <P>SOLUTION: The antioxidant, the active oxygen scavenger and the radical scavenger contain an extract from Telosma cordata as the active ingredient, and the extract is also added to the skin cosmetics, and the foods and drinks for beauty. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  TECHNICAL FIELD The present invention relates to an antioxidant, an active oxygen scavenger and a radical scavenger containing a plant extract as an active ingredient, and a skin cosmetic and a cosmetic food and drink containing the plant extract.

In recent years, active oxygen has attracted attention as a factor that particularly oxidizes biological components, and its adverse effect on living organisms has become a problem. Active oxygen is generated in the process of energy metabolism in living cells, and is superoxide (ie, superoxide anion (· O ²⁻ ) generated by one-electron reduction of oxygen molecules), hydrogen peroxide (H ₂ O ₂ ), There are singlet oxygen ( ¹ O ₂ ), hydroxy radical (.OH), and the like. These active oxygens are essential for the phagocytic sterilization mechanism and play an important role in the removal of viruses and cancer cells. However, excessive generation of active oxygen attacks in vivo molecules that make up membranes and tissues in the body. There is a risk of inducing various diseases.

  For example, active oxygen is thought to decompose, denature or crosslink biological tissues such as collagen, or to produce lipid peroxides that oxidize fats and oils and damage cells. It is considered that this disorder causes aging such as skin wrinkle formation and skin elasticity reduction, inflammation, skin pigmentation, and the like (see Non-Patent Document 1).

  Therefore, skin aging such as formation of skin wrinkles and decreased elasticity, skin inflammation, skin inflammation, skin pigmentation, etc. through inhibition and suppression of generation of active oxygen and biological radicals, etc. Can be prevented and treated.

Therefore, attempts have been made to obtain a substance having an active oxygen scavenging action or a radical scavenging action from a natural product advantageous in terms of safety. For example, the fruit juice of Togenashi (see Patent Document 1), Melissa, Enmiso, and birch , Minami Tenmi, Kina and Ages (see Patent Document 2), Vulcania sashimi and Shirakashi (see Patent Document 3), Flavonoids such as Astilbine and Taxifolin (see Patent Document 4), Fuji Tea (see Patent Document 5), etc. It has been confirmed.
JP-A-3-83548 JP-A-3-157334 JP-A-5-316963 JP-A-6-65074 JP 2001-97873 A Fragrance Journal Special Issue No. 14 156-161 1995

  The present invention finds an easily obtainable plant extract that has not been known to have an active oxygen scavenging action and / or radical scavenging action, and uses a highly safe antioxidant, active oxygen scavenger, It aims at providing a radical scavenger, skin cosmetics, and cosmetics food and drink.

  In order to achieve the above object, first, the present invention includes an antioxidant (Claim 1) and an active oxygen scavenger (Claim) characterized by containing an extract from Teleisma cordata as an active ingredient. Item 3) and a radical scavenger (Claim 4), and particularly an antioxidant (Claim 2), wherein the extract has an active oxygen scavenging action and / or a radical scavenging action.

  Secondly, the present invention provides a skin cosmetic (Claim 5) and a cosmetic food and drink (Claim 6), characterized by blending an extract from Yelosian (Telosma cordata).

  ADVANTAGE OF THE INVENTION According to this invention, the safe antioxidant, active oxygen scavenger, radical scavenger, skin cosmetics, and cosmetics food / beverage products using the easily available plant extract can be provided. According to the antioxidant, the active oxygen scavenger, the radical scavenger, the skin cosmetic, or the cosmetics food and drink according to the present invention, through the prevention of the oxidation of the biological components by the active oxygen scavenging action and the in vivo radical scavenging action, Aging phenomena such as wrinkle formation and reduced elasticity, as well as skin inflammation and skin pigmentation can be effectively prevented and treated.

Hereinafter, the present invention will be described in detail.
The antioxidant, the active oxygen scavenger and the radical scavenger according to the present invention all contain an extract from a yellowtail as an active ingredient.

  Here, the “extract” from the yellowtailer includes the extract obtained from the yellowtail by the extraction process, a diluted or concentrated solution of the extract, a dry product obtained by drying the extract, or a crude product thereof. Both products and purified products are included.

  Yerishan (Yoriko) is a potato family plant with the scientific name "Telosma cordata" and grows naturally from southern China to India and is readily available from these regions.

  Examples of the constituent parts of the Yereshan used as the extraction raw material include leaf parts, stem parts, flower parts, seeds, or mixed parts thereof (hereinafter collectively referred to as “terrestrial part”) or root parts. In particular, it is preferable to use the ground part of a yellowtailer. Here, the "flower" generally refers to the whole organ involved in sexual reproduction of a seed plant, and is composed of a flower leaf that is a leaf deformation and a flower axis that is a stem deformation. It includes organs such as pupae, petals, stamens, and heart skin. The “floral part” used as an extraction raw material in the present invention includes all of the organs involved in sexual reproduction of seed plants, as well as parts thereof, such as flower leaves, flower coats (buds and corolla), corolla, petals, etc. Is also included.

  Although details of the substance having an active oxygen scavenging action and / or radical scavenging action contained in the extract from the yellow fish are unknown, the active oxygen scavenging action from the yellow fish and by the extraction method generally used for the extraction of plants. An extract having a radical scavenging action can be obtained. For example, it can be obtained by drying the raw material for extraction and then pulverizing it as it is or using a crusher and subjecting it to extraction with an extraction solvent. At this time, the extraction raw material may be dried in the sun or using a commonly used dryer. In addition, the yellowtail may be used as an extraction raw material after pretreatment such as degreasing with a nonpolar solvent such as hexane or benzene. By performing a pretreatment such as degreasing, extraction with a polar solvent of a yellowtail can be efficiently performed.

  As the extraction solvent, it is preferable to use a polar solvent, and it is particularly preferable to use water, a hydrophilic organic solvent, or a mixture thereof at room temperature or a temperature not higher than the boiling point of the solvent.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as water that has been subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, the water that can be used as the extraction solvent in the present invention includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  Examples of the hydrophilic organic solvent that can be used as the extraction solvent include lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin.

  When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when using a mixed solution of water and a lower alcohol, 1 to 90 parts by weight of a lower alcohol with respect to 10 parts by weight of water, and when using a mixed solution of water and a lower aliphatic ketone, When using a mixed liquid of 1 to 40 parts by weight of a lower ketone and 10 parts by weight of water and a polyhydric alcohol, 1 to 90 parts by weight of a polyhydric alcohol is mixed with 10 parts by weight of water. preferable.

  The extraction treatment is not particularly limited as long as the soluble component contained in the yellowtail can be eluted in the extraction solvent, and can be performed according to a conventional method. In the extraction process, it is not necessary to adopt a special extraction method, and any apparatus can be used at room temperature or under reflux heating.

  Specifically, the extraction raw material is put into a processing tank filled with the extraction solvent, and after standing for 30 minutes to 4 hours usually with occasional stirring to elute the soluble components, it is filtered or centrifuged. To remove solids and obtain an extract. The amount of extraction solvent is usually 5 to 15 times (mass ratio) of the extraction raw material. The extraction conditions are usually about 1 to 4 hours at 50 to 95 ° C. when water is used as the extraction solvent, and usually 40 to 80 when a mixed solvent of water and ethanol is used as the extraction solvent. It is about 30 minutes to 4 hours at ° C.

  When the solvent is distilled off from the obtained extract, a paste-like concentrate is obtained, and when this concentrate is further dried, a solid extract is obtained. However, the extract from the Yereshan does not have to be a solid extract, and may be in the state of the above extract or its concentrate. Extracts from these yellowtails may be used after being purified by a method such as activated carbon treatment, adsorption resin treatment, ion exchange resin, liquid-liquid countercurrent distribution, etc., as long as the physiological activity is not reduced.

  The extract from the house fisherman has no unpleasant odor and does not have the color tone peculiar to the extract, so it can be used as it is as an antioxidant, active oxygen scavenger or radical scavenger, but it is concentrated and dried. It can be used in a dosage form, and if necessary, it can be purified for the purpose of improving the activity and decoloring / deodorizing, or can be mixed with an optional auxiliary agent and used.

  Formulation of an extract from a yellowtail can be carried out according to a conventional method. For formulation, in order to facilitate storage and handling, pharmaceutically acceptable carriers such as dextrin and cyclodextrin and other optional auxiliaries can be added. It can be formulated into any dosage form such as granular or tablet.

  The extract from the yellowtail obtained as described above has an active oxygen scavenging action and / or a radical scavenging action, and these actions are used to effectively use an antioxidant, an active oxygen scavenger or a radical scavenger. Can be used as an ingredient.

  Here, the antioxidant effect of the extract from the yellowtail is exhibited based on the active oxygen scavenging action and / or the radical scavenging action. “Active oxygen” includes superoxide, hydrogen peroxide, hydroxy radical, singlet oxygen and the like. The term “radical” means a molecule or atom having one or more unpaired electrons, and includes superoxide, hydroxy radical, DPPH and the like.

  Extracts from yellowtails, and antioxidants, active oxygen scavengers and radical scavengers containing the extracts as active ingredients prevent and / or improve skin aging, skin inflammation and skin pigmentation It is suitable for blending into skin cosmetics because it is easy to use and safe when applied to the skin.

  There are no particular limitations on the types of skin cosmetics that can be blended with the extract from the Yelaishan. Specific examples thereof include ointments, creams, emulsions, lotions, packs, lip balms, bath salts, hair arts, hair lotions, soaps, Examples include body shampoos.

  The blending amount of the extract from the yerishan in the skin cosmetics of the present invention can be appropriately adjusted according to the type of skin cosmetics, physiological activity of the extract, etc., but the preferred blending ratio is that of the standard extract. In this case, the amount is about 0.0001 to 10% by mass in terms of dry matter.

  The skin cosmetics of the present invention include various main agents and auxiliaries usually used in the production of skin cosmetics, as long as they do not interfere with the active oxygen scavenging action and / or radical scavenging action of the extract from the house chef. Auxiliaries can be used. The skin cosmetic of the present invention is not limited to the one in which only an extract from a yellowtail is used as a main agent with regard to prevention and improvement of skin aging.

  Examples of the skin cosmetic composition of the present invention that can be used as a constituent component of the skin cosmetic together with the extract from the yerishan are, for example, astringents, bactericides / antibacterial agents, whitening agents, ultraviolet absorbers, moisturizers, cell activators. , Anti-allergic agents, anti-oxidant / active oxygen scavengers, etc., and when the above components are used in combination with an extract from a fisherman, a synergistic effect between the component from the extract from the fisherman However, there are cases in which it is possible to provide a superior use effect than normally expected.

  When producing skin cosmetics containing extracts from yellowtailers, the choice of other production raw materials is rarely limited, and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, Any of general substrates and auxiliaries such as surfactants and fragrances can be used.

  Since it has been confirmed that the extract from the yellowtail is not digested in the digestive tract, it is suitable for blending into any food or drink supplement. In this case, it is appropriate that the amount of the extract per day for adults is about 1 to 1000 mg in consideration of the general intake of the food and drink to be added.

  The food / beverage products containing the extract from the yellowtail are imparted with an action to prevent and / or improve skin aging, skin inflammation and skin pigmentation, and can be used as cosmetic food / beverage products. Here, the “beauty food and drink” means food and drink aimed at preventing and / or improving the formation of beautiful skin, skin aging, skin inflammation and skin pigmentation.

  The cosmetic food and drink according to the present invention may be a food or drink prepared by blending an extract from a house chef with any food or drink that does not impede its activity. It may be.

  When producing the cosmetic food and drink of the present invention, for example, sugars such as dextrin and starch; proteins such as gelatin, soybean protein and corn protein; amino acids such as alanine, glutamine and isoleucine; cellulose and gum arabic Polysaccharides: It can be formulated into an arbitrary dosage form by adding optional auxiliaries such as soybean oil and oils such as medium chain fatty acid triglycerides.

  Foods and drinks that can be blended with the extract from the yerishan are not particularly limited, but specific examples include soft drinks, carbonated drinks, nutritional drinks, fruit drinks, lactic acid drinks and the like (concentrated concentrates and preparations for these drinks) Ice cream, ice sherbet, shaved ice, etc .; noodles such as buckwheat, udon, harusame, gyoza skin, sushi mai, Chinese noodles, instant noodles; rice cake, chewing gum, candy, gum, chocolate, tablets Confectionery such as confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery; processed fishery and livestock products such as kamaboko, ham, sausage; dairy products such as processed milk, fermented milk; salad oil, tempura oil, margarine, mayonnaise , Shortening, whipped cream, dressing and other fats and oils and processed foods; seasonings such as sauces and sauces ; Soups, stews, salads, prepared foods, pickles, and the like.

  The antioxidants, active oxygen scavengers, radical scavengers, skin cosmetics and cosmetic foods and beverages of the present invention described above are suitably applied to humans, but their respective effects are achieved. As long as it can be applied to animals other than humans.

  Hereinafter, although a manufacture example, a test example, and a compounding example are shown and this invention is demonstrated concretely, this invention is not limited to each following example at all.

[Production Example 1]
An extraction solvent of 2000 mL was added to 200 g of the above ground portion of a yellow fisher, and the mixture was heated and extracted with a reflux extractor at 80 ° C. for 2 hours and filtered while hot to obtain an extract. The residue was further subjected to the same extraction treatment, and the obtained extract and the above extract were combined, concentrated under reduced pressure, and further dried to obtain an extract. Table 1 shows the extraction rates when water, 50% ethanol (mass ratio of water to ethanol of 1: 1) and ethanol were used as the extraction solvent.

[Table 1]
Extraction solvent extraction rate (%)
Water 21.5
50% ethanol 27.1
Ethanol 19.8

[Test Example 1] Superoxide elimination test (NBT method)
The extract from the above-ground portion of the yellowtail obtained in Production Example 1 (hereinafter referred to as “sample”) was tested for superoxide scavenging action by the following test method.

0.1 mL each of 3 mM xanthine, 0.05 M Na ₂ CO ₃ buffer (pH 10.2), 3 mM EDTA, 1.5 mg / mL BSA solution, and 0.75 mM nitroblue tetrazolium (NBT) The sample solution was added to a test tube, and 0.1 mL of the sample solution was added thereto, and left at 25 ° C. for 10 minutes. Next, 0.1 mL of a xanthine oxidase solution was added, stirred rapidly, and allowed to stand at 25 ° C. for 20 minutes. Thereafter, 0.1 mL of 6 mM copper chloride was added to stop the reaction, and the absorbance at a wavelength of 560 nm was measured. Hereinafter, this absorbance is referred to as “absorbance upon addition of sample solution and enzyme solution”.

  The same operation and measurement of absorbance were performed without adding the enzyme solution. The absorbance measured at this time is referred to as “absorbance when the sample solution is added and the enzyme solution is not added”.

  On the other hand, the same measurement was performed when distilled water was added without adding the sample solution. The absorbance measured at this time is referred to as “absorbance when no sample solution is added and the enzyme solution is added”.

  The same measurement was performed when distilled water was added without adding the enzyme solution and without adding the sample solution. The absorbance measured at this time is referred to as “absorbance when no sample solution is added and no enzyme solution is added”.

And the superoxide elimination rate was calculated | required by following Formula.
Erase rate (%) = {1− (A−B) / (C−D)} × 100
In the above formula, “A” is “absorbance when sample solution is added and enzyme solution is added”, “B” is “absorbance when sample solution is added and enzyme solution is not added”, and “C” is “sample solution is not added and enzyme is added” “Absorptivity when solution is added” and “D” represent “absorbance when sample solution is not added and enzyme solution is not added”.

  The above-mentioned erasure rate was measured by decreasing the sample concentration stepwise, and the sample concentration (ppm; μg / mL) at which the superoxide erasure rate was 50% was determined by interpolation. The results are shown in Table 2.

[Table 2]
Sample (extraction solvent) 50% inhibitory sample concentration (ppm)
Water 85.2
50% ethanol 43.2
Ethanol 93.1

  From the results shown in Table 2, it was confirmed that the extract from the above-ground part of the yellow fish had superoxide scavenging action.

[Test Example 2] Radical scavenging test for DPPH For the extract from the above-ground part of the yellow seaweed obtained in Production Example 1 (hereinafter referred to as "sample"), DPPH, which is a very stable radical, was used by the following test method. Then, radical scavenging action was tested.

3 mL of the sample solution was added to 3 mL of 1.5 × 10 ⁻⁴ M DPPH ethanol solution, and the container was immediately sealed, shaken and allowed to stand for 30 minutes. Thereafter, absorbance at a wavelength of 520 nm was measured. As a control, the same operation was performed using a solvent in which the sample solution was dissolved instead of the sample solution, and the absorbance at a wavelength of 520 nm was measured. Further, as a blank, after adding 3 mL of the sample solution to ethanol, the absorbance at a wavelength of 520 nm was measured immediately. From each measured absorbance, the radical scavenging rate (%) was calculated by the following formula.

Erase rate (%) = {1− (B−C) / A} × 100
In the above formula, “A” represents “control absorbance”, “B” represents “absorbance when a sample solution is added”, and “C” represents “blank absorbance”.

  The above-mentioned erasure rate was measured by gradually decreasing the sample concentration, and the sample concentration (ppm; μg / mL) at which the DPPH radical erasure rate was 50% was determined by interpolation. The results are shown in Table 3.

[Table 3]
Sample (extraction solvent) 50% inhibitory sample concentration (ppm)
Water> 200
50% ethanol 183.1
Ethanol 195.5

  From the results shown in Table 3, it was confirmed that the extract from the above-ground part of the yellow fish has a radical scavenging action.

[Formulation Example 1]
An emulsion having the following composition was produced by a conventional method.
Yeraishan Water Extract 1.0g
Jojoba oil 4.0g
Placenta extract 0.1g
Ascorbic acid magnesium phosphate 0.1g
Prune extract 1.0g
Tounin extract 1.0g
Kyonin extract 1.0g
Taio extract 0.5g
Cousin extract 0.5g
Olive oil 2.0g
Squalane 2.0g
Cetanol 2.0g
Glyceryl monostearate 2.0g
Polyoxyethylene cetyl ether (20E.O.) 2.5 g
Oleic acid polyoxyethylene sorbitan (20E.O.) 2.0 g
1,3-butylene glycol 3.0 g
Hinokitiol 0.15g
Fragrance 0.15g
Purified water remainder (total amount is 100 g)

[Formulation Example 2]
A cream having the following composition was produced by a conventional method.
Yellowtail 50% ethanol extract 5.0g
Liquid paraffin 5.0g
Salami beeswax 4.0g
Cetanol 3.0g
Squalane 10.0g
Lanolin 2.0g
Arbutin 0.1g
Lucinol 0.1g
Twilight extract 0.1g
Ginkgo biloba extract 0.1g
Conchiolin 0.1g
Oat extract 0.1g
Stearic acid 1.0g
Oleic acid polyoxyethylene sorbitan (20E.O.) 1.5g
3.0 g glyceryl monostearate
1,3-butylene glycol 6.0 g
0.05 g of methyl paraoxybenzoate
Fragrance 0.1g
Purified water remainder (total amount is 100 g)

[Composition Example 3]
A pack having the following composition was produced by a conventional method.
0.5g of Yeraishan ethanol extract
Polyvinyl alcohol 15.0g
Polyethylene glycol 3.0g
Propylene glycol 7.0g
Ethanol 10.0g
Chamomile extract 0.1g
Ellagic acid 0.1g
Oil soluble licorice extract 0.1g
Seaweed extract 0.1g
Yeast extract 0.1g
Perilla extract 0.1g
Warmoko extract 0.1g
Linden extract 0.1g
0.05 g ethyl paraoxybenzoate
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

Antioxidants, active oxygen scavengers, radical scavengers, skin cosmetics and beauty foods and beverages according to the present invention are used to prevent skin aging, skin oxidation through prevention of oxidation of biological components by active oxygen scavenging action and in vivo radical scavenging action. It is useful for preventing and / or improving skin inflammation and skin pigmentation.

Claims (6)

  An antioxidant comprising an extract from yellowtail (Telosma cordata) as an active ingredient.   The antioxidant according to claim 1, wherein the extract has an active oxygen scavenging action and / or a radical scavenging action.   An active oxygen scavenger characterized by containing an extract from yellowtail (Telosma cordata) as an active ingredient.   Radical scavenger characterized by containing an extract from yellowtail (Telosma cordata) as an active ingredient.   A skin cosmetic characterized by containing an extract from Teleisma cordata. Beauty food and drink characterized by blending an extract from Yelosian (Telosma cordata).