JP2007016003A - Antioxidant - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an antioxidant, an active oxygen scavenger, a radical scavenger, a skin cosmetic, and a food for beauty each having high safety, by adding a plant extract having anti-oxidizing, active oxygen-removing, and radical-scavenging activity thereto, so as to utilize the activity, namely, antioxidant, active oxygen scavenging, and radical scavenging activity, by discovering a material having the active oxygen scavenging-activity and/or the radical-scavenging activity from a natural product and adding the material thereto as an active ingredient, and to provide the skin cosmetic and the food for beauty compounded of the material. <P>SOLUTION: The antioxidant, the active oxygen scavenger, or the radical scavenger of which the each contains an extract from a butterfly pea as an active ingredient is provided as a preparation. Further, the skin cosmetic or the food for beauty of which the each is compounded of the extract from the butterfly pea is provided. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  TECHNICAL FIELD The present invention relates to an antioxidant, an active oxygen scavenger and a radical scavenger containing an extract from butterfly beans as an active ingredient, and a skin cosmetic and a cosmetic food and drink containing the extract from butter beans. .

  In recent years, it has become clear that active oxygen generated in the living body has an adverse effect on the human body and is associated with various diseases, carcinogenesis, and aging. Active oxygen is generated in the process of energy metabolism in living cells, and includes superoxide (that is, superoxide anion generated by one-electron reduction of oxygen molecules, hydrogen peroxide, singlet oxygen, hydroxy radical, etc.). Is essential for the phagocytic sterilization mechanism and plays an important role in the removal of viruses and cancer cells. However, excessive production of active oxygen attacks in vivo molecules that make up membranes and tissues in the body, causing various diseases. In particular, since the skin is in the outermost layer of the living body, it is directly subject to environmental factors such as ultraviolet rays and free radicals. The effects such as the increase in color, abnormal pigmentation such as spots and freckles, inflammation, and aging are very remarkable.

  Conventionally, attempts have been made to prevent oxidative damage by blending an anti-oxidant with an external preparation for skin. For example, as such an antioxidant, tocopherol (vitamin E), ascorbic acid (vitamin C), butylhydroxyanisole (BHT), butylhydroxytoluene (BHA), sesamol dimer (JP 2001-139944 A) (patent document) 1) and the like, but these synthetic antioxidants have safety problems, and the addition amount is limited. From this point of view, natural antioxidants, Active oxygen scavengers, radical scavengers, skin cosmetics and beauty foods and beverages are desired.

JP 2001-139944 A

  The present invention solves the conventional problems, and contains a plant extract having antioxidant, active oxygen scavenging and radical scavenging action, thereby making it possible to use a highly safe antioxidant, active oxygen scavenger, It aims at providing a radical scavenger, skin cosmetics, and cosmetics food and drink.

  The present invention relates to an antioxidant containing an extract from butterfly as an active ingredient.

  The present invention also relates to the above antioxidant, wherein the extract from butterfly has an active oxygen scavenging action and / or a radical scavenging action.

  The present invention also relates to an active oxygen scavenger comprising an extract from butterfly beans as an active ingredient.

  Moreover, this invention relates to the radical scavenger characterized by including the extract from butterfly bean as an active ingredient.

  Moreover, this invention relates to the skin cosmetics characterized by mix | blending the extract from butterfly beans.

  Moreover, this invention relates to the food-drinks for cosmetics characterized by mix | blending the extract from butterfly beans.

  ADVANTAGE OF THE INVENTION According to this invention, the safe antioxidant, active oxygen scavenger, radical scavenger, skin cosmetics, and cosmetics food / beverage using the extract from butterfly beans which can be obtained can be provided. According to the antioxidants, active oxygen scavengers, radical scavengers, skin cosmetics or cosmetic foods and drinks obtained in the present invention, through the prevention of oxidation of biological components by active oxygen scavenging action and in vivo radical scavenging action, the skin It is possible to effectively prevent and treat aging phenomena such as wrinkle formation and reduced elasticity, as well as skin inflammation and skin pigmentation.

  The antioxidant, the active oxygen scavenger and the radical scavenger according to the present invention all contain an extracted component from butterfly as an active ingredient.

  As a constituent part of butterfly beans used as an extraction raw material, a leaf part, a stem part, a flower part, a seed, or a mixed part thereof (hereinafter collectively referred to as “terrestrial part”) or a root part may be mentioned. Among these, it is particularly preferable to use the above-ground part. Here, "flower" generally refers to the total organs involved in sexual reproduction of seed plants, and is composed of flower leaves that are leaf deformations and flower axes that are stem deformations. It includes organs such as pupae, petals, stamens, and heart skin. The “floral part” used as an extraction raw material in the present invention includes not only the whole organs involved in sexual reproduction of seed plants but also some of them, such as flower leaves, flower coats (buds and corolla), corolla, petals, etc. included.

The details of the substance having an active oxygen scavenging action and / or radical scavenging action contained in the extract from butterfly beans are unknown, but the extract from butterfly beans is extracted by an extraction method generally used for plant extraction. An extract having an active oxygen scavenging action and / or a radical scavenging action contained in can be obtained. For example, it can be obtained by drying the raw material for extraction and then pulverizing it as it is or using a pulverizer and subjecting it to extraction with an extraction solvent.
Therefore, the extract from butterfly beans used in the present invention does not necessarily have to contain a flower-derived one. Since the thing derived from a flower part contains a vivid blue pigment | dye, the thing from which this blue pigment | dye was removed as needed may be sufficient.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as those subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, and buffering.

  Examples of the hydrophilic organic solvent that can be used as the extraction solvent include lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol, lower aliphatic ketones such as acetone and methyl ethyl ketone, butylene glycol, and propylene glycol. And polyhydric alcohols having 2 to 5 carbon atoms such as glycerin.

  The above extraction solvents can be used in combination of two or more. For example, when using a mixed solution of water and lower alcohol, 1 to 90 parts by weight of lower alcohol with respect to 10 parts by weight of water, and when using a mixed solution of water and lower aliphatic ketone, It can adjust suitably similarly about 1-90 weight part of lower ketone and the liquid mixture of water and a polyhydric alcohol with respect to 10 weight part.

  The extraction treatment is not particularly limited as long as the soluble component contained in butterfly can be eluted in the extraction solvent, and can be performed according to a conventional method. In the extraction process, it is not necessary to adopt a special extraction method, and any apparatus can be used at room temperature or under reflux heating.

  The butterfly extract may be used as it is, but may be filtered and concentrated as necessary. The extract can also be used after being fractionated and purified by a column chromatography method or the like. Furthermore, after the extract is dried under reduced pressure or freeze-dried, it can be appropriately formulated and used, for example, by adjusting it to a powder or paste.

  The extract from butterfly beans obtained as described above has an active oxygen scavenging and / or radical scavenging action. Using these actions, an antioxidant, an active oxygen scavenger, or a radical scavenger can be used. It can be used as an active ingredient.

  Here, the antioxidant action of the extract from butterfly beans is exhibited based on the active oxygen scavenging action and / or the radical scavenging action. “Active oxygen” includes superoxide, hydrogen peroxide, hydroxy radical, singlet oxygen and the like. The term “radical” means a molecule or atom having one or more unpaired electrons, and includes superoxide, hydroxy radical, DPPH and the like.

  Extracts from butterfly beans and antioxidants, active oxygen scavengers and radical scavengers containing the extracts as active ingredients prevent and / or improve skin aging, skin inflammation and skin pigmentation In addition, it is suitable for blending into skin cosmetics because of its excellent usability and safety when applied to the skin.

  There are no particular limitations on the types of skin cosmetics that can be combined with the extract from butterfly beans. , Body shampoos, and the like.

  The blending amount of the extract from butterfly beans in the skin cosmetic of the present invention can be appropriately adjusted depending on the type of skin cosmetic and the physiological activity of the extract, preferably, in the case of a standard extract, It is 0.0001-10 weight% of the whole cosmetics in terms of dry matter.

  The skin cosmetic of the present invention includes various main agents and auxiliaries usually used in the production of skin cosmetics, as long as it does not interfere with the active oxygen scavenging action and / or radical scavenging action of the extract from butterfly beans. Any auxiliary can be used. The skin cosmetic of the present invention is not limited to the one in which only the extract from butterfly beans is the main agent for preventing and improving skin aging.

  In the skin cosmetics of the present invention, examples of the cosmetic constituents that can be used together with the extract from butterfly include, for example, bactericidal antibacterial agents, whitening agents, ultraviolet absorbers, moisturizers, cell bioactive agents, anti-inflammatory / antiallergic agents. Agents, active oxygen scavengers, and the like, and when used in combination with an extract from butterfly beans, the synergistic effect with the components used in combination may lead to an excellent use effect that is normally expected.

  When producing skin cosmetics containing an extract from butterfly beans, the choice of other raw materials for production is rarely limited. Oils, waxes, hydrocarbons, fatty acids, alcohols, esters Any general base materials and auxiliaries such as surfactants and fragrances can be used.

  Since the extract of butterfly beans has been confirmed to be safe even if it is eaten, it is suitable for blending into any food or drink or nutritional supplement. In this case, the amount of extract per day for an adult is preferably 1 to 3500 mg / Kg in consideration of the general intake of the food to be added.

  Foods and drinks containing extracts from butterfly beans are imparted with an action to prevent and / or improve skin aging, skin inflammation and skin pigmentation, and can be used as cosmetic foods and drinks. . Here, the term “beauty food / beverage” means a food / beverage intended to prevent and / or improve the formation of beautiful skin or skin aging, skin inflammation and skin deposition.

  The beauty food or drink of the present invention may be a blend of an extract from butterfly beans in any food or drink that does not impede its activity, or nutrition based on an extract from butterfly beans. It may be a supplement.

  In producing the cosmetic food and drink of the present invention, for example, sugars such as dextrin and starch, proteins such as gelatin, soybean protein and corn protein, amino acids such as alanine, glutamine and isoleucine, cellulose and gum arabic and the like Arbitrary auxiliaries such as polysaccharides, soybean oil, oils and fats such as medium-chain fatty acid triglycerides can be added to prepare a dosage form.

  Foods and drinks that can be mixed with the extract from butterfly beans are not particularly limited, for example, beverages such as soft drinks, carbonated drinks, nutritional drinks, fruit drinks, lactic acid drinks, ice cream, ice sherbet, frozen confectionery such as shaved ice, soba , Udon, Harusame, dumpling skin, Shumai skin, Chinese noodles, instant noodles and other noodles, rice cakes, chewing gum, candy, gum, chocolate, tablet candy, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, etc. Processed fishery products such as seafood, salmon, ham, sausage, dairy products such as processed milk and fermented milk, salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing and other fats and oils processed foods, sauces, Seasonings such as sauce, soups, stews, salads, prepared dishes, and pickles are listed.

  The antioxidants, active oxygen scavengers, radical scavengers, skin cosmetics and cosmetic foods and beverages of the present invention described above are suitably applied to humans, but their respective effects are obtained. Insofar as it can be applied to animals other than humans.

  Examples of the present invention will be specifically described below, but the present invention is not limited to these examples. In the description,% represents% by weight and part represents part by weight.

[Production Example 1]
To 200 g of dried butterfly beans above, 2000 ml of an extraction solvent was added, and the mixture was heated and extracted with a reflux extractor at 80 ° C. for 2 hours, and filtered while hot to obtain an extract. The residue was further subjected to the same treatment, and the obtained extract and the above extract were combined and concentrated under reduced pressure, and further dried to obtain an extract. Table 1 shows the extraction rate when pure water, 50% ethanol and ethanol were used as the extraction solvent.

[Test Example 1] Superoxide elimination test (NBT method)
The extract from the ground of butterfly beans obtained in Production Example 1 (hereinafter referred to as “sample”) was tested for superoxide scavenging action as follows.

  Take 3 mM xanthine, 0.05 M sodium carbonate buffer (pH 10), 3 mM EDTA, 1.5 mg / ml BSA solution, and 0.75 mM nitroblue tetrazolium (NBT) each in a 0.1 ml tube, To this, 0.1 ml of the sample solution was added and left at 25 ° C. for 10 minutes. Next, 0.1 ml of a xanthine oxidase solution was added, stirred rapidly, and allowed to stand at 25 ° C. for 20 minutes. Thereafter, 0.1 ml of 6 mM copper chloride was added to stop the reaction, and the absorbance at a wavelength of 560 nm was measured. Hereinafter, this absorbance is referred to as “absorbance upon addition of sample solution and enzyme solution” = A.

  The same operation and measurement of absorbance were performed without adding the enzyme solution. The absorbance at this time is referred to as “absorbance when the sample solution is added and no enzyme solution is added” = B.

  On the other hand, the same measurement was performed when distilled water was added without adding the sample solution. The absorbance at this time is referred to as “absorbance when no sample solution is added and enzyme solution is added” = C.

  Further, the same measurement was performed when distilled water was used without adding both the sample solution and the enzyme solution. The absorbance at this time is referred to as “absorbance when no sample solution is added and no enzyme solution is added” = D.

The superoxide elimination rate was determined by the following formula.
Erase rate (%) = {1− (A−B) / (C−D)} × 100

  The above-mentioned erasure rate was measured by decreasing the sample concentration stepwise, and the sample concentration (ppm; microg / ml) at which the superoxide erasure rate was 50% was determined by interpolation. The results are shown in Table 2.

  From the results in Table 2, it was confirmed that the extract from the above-ground part of butterfly had superoxide scavenging action.

[Test Example 2] Radical scavenging test for DPPH The extract from the above-ground parts of butterfly obtained in Production Example 1 (hereinafter referred to as "sample") has a radical scavenging action using DPPH by the following test method. Tested.

3 ml of the sample solution was added to 3 ml of 1.5 × 10 ⁻⁴ M DPPH ethanol solution, and the container was immediately sealed, shaken and allowed to stand for 30 minutes. Thereafter, the absorbance at a wavelength of 520 nm was measured. As a control, the same operation was performed using a solvent in which the sample solution was dissolved instead of the sample solution, and the absorbance at a wavelength of 520 nm was measured. As a blank, after adding 3 ml of the sample solution to ethanol, the absorbance at a wavelength of 520 nm was measured immediately. From each measured absorbance, the radical scavenging rate (%) was calculated by the following formula.
Erase rate (%) = {1− (B−C) / A} × 100
In the above formula, “A” is “absorbance of control”, “B” is “absorbance when sample solution is added”, and “C” is “absorbance of blank”.

  The above-mentioned erasure rate was measured by gradually reducing the sample concentration, and the sample concentration (ppm; microg / ml) at which the DPPH radical erasure rate was 50% was determined by interpolation. The results are shown in Table 3.

  From the result of Table 3, it was confirmed that the extract from the above-ground part of butterfly has a radical scavenging action.

[Formulation Example 1]
Toner lotion Formulation: 1. Ethanol 5.0 parts 2. Methyl P-oxybenzoate 0.1 part 3. Polyoxyethylene (40) hydrogenated castor oil 0.1 part Perfume appropriate amount 5. Purified water 10.0 parts 6. Ethanol extract of butterfly petals 5.0 parts 7. 3.0 parts of 1,3-butylene glycol 8. Glycerin 2.0 parts 9. Xanthan gum 0.02 part 10. Purified water 74.5 parts Production method: Components 1 to 5 and components 6 to 10 are uniformly dissolved, mixed and filtered to obtain a product.

[Formulation Example 2]
Cream formulation Formulation: 1. Stearic acid 4.0 parts 2. Cetyl alcohol 3.0 parts Stearyl alcohol 1.0 part 4. Liquid paraffin 6.5 parts 5. Vaseline 10.0 parts 6. Sorbitan monostearate 2.5 parts 7. Polyoxyethylene (25) monostearate 3.0 parts 8. 1,3-butylene glycol 5.0 parts 9. Potassium hydroxide 0.1 part 10. 10. Hot water extract 0.2 parts above ground butterfly Methyl P-oxybenzoate 0.2 part 12. Purified water 64.5 parts 13. Fragrance Appropriate amount Production method: Oil phase components 1 to 7 and water phase components 8 to 12 are heated and dissolved at 70 to 75 ° C., and then added to oil phase components 1 to 7 and emulsified by cooling. Ingredient 13 is added and mixed, and cooled to 30 ° C. to obtain a product.

[Composition Example 3]
Emulsion formulation Formulation: 1. Stearic acid 2.0 parts 2. Stearyl alcohol 2.0 parts Liquid paraffin 8.0 parts 4. 4. Glycerin monostearate 2.3 parts Sorbitan monooleate 2.5 parts 6. 6. Polyoxyethylene (10) sorbitan monooleate 0.8 part Glycerin 6.0 parts 8. 8. 0.5 parts of 50% ethanol extract of above ground butterfly 9. 9. Methyl P-oxybenzoate 0.2 part Purified water 77.0 parts 11. Perfume Appropriate amount Production method: Oil phase components 1 to 7 and water phase components 8 to 10 are heated and dissolved at 70 to 75 ° C., respectively, and then added to oil phase components 1 to 7 and emulsified by cooling. Ingredient 11 is added and mixed, and cooled to 30 ° C. to obtain a product.

[Formulation Example 4]
Pack formulation Formulation: 1. Polyvinyl alcohol 12.0 parts Ethyl alcohol 5.0 parts 3. 1,3-butylene glycol 8.0 parts 4. Methyl P-oxybenzoate 0.2 part 5. Polyoxyethylene (40) hydrogenated castor oil 0.5 part 6. 1.0 parts water extract of butterfly petal Citric acid 0.1 part 8. Sodium citrate 0.3 part 9. Perfume appropriate amount 10. Purified water 73.8 parts Manufacturing method: Dissolve each component uniformly to make a product.

[Formulation Example 5]
Hair cream formulation Formulation: 1. Stearic acid 2.0 parts 2. Stearyl alcohol 2.0 parts Liquid paraffin 8.0 parts 4. 4. Glycerin monostearate 2.3 parts Sorbitan monooleate 2.5 parts 6. 6. Polyoxyethylene (10) sorbitan monooleate 0.8 part Hot water extract 0.4 parts above butterfly beans 8. Glycerin 6.0 parts 9. Methyl P-oxybenzoate 0.1 part Purified water 75.9 parts 11. Perfume Appropriate amount Production method: Dissolve oil phase components 1 to 6 and water phase components 7 to 10 at 70 to 75 ° C., respectively. Water phase components 7 to 10 are added to oil phase components 1 to 6 to emulsify, and component 11 is added and mixed during cooling, and cooled to 30 ° C. to obtain a product.

[Composition Example 6]
Bath preparation formulation Sodium sulfate 43.0 parts 2. Sodium bicarbonate 50.0 parts 3. 5.0 parts ethanol extract of butterfly petals Perfume Appropriate amount Production method: Mix each component well to make a product.

Antioxidants, active oxygen scavengers, radical scavengers, skin cosmetics and beauty foods and beverages of the present invention are used for skin aging, skin prevention through prevention of oxidation of biological components by active oxygen scavenging action and in vivo radical scavenging action. It is useful for preventing and / or improving skin inflammation and skin pigmentation.

Claims (6)

  An antioxidant comprising an extract from butterfly beans as an active ingredient.   2. The antioxidant according to claim 1, wherein the extract from butterfly has an active oxygen scavenging action and / or a radical scavenging action.   An active oxygen scavenger characterized by containing an extract from butterfly beans as an active ingredient.   A radical scavenger comprising an extract from butterfly beans as an active ingredient.   Skin cosmetic characterized by containing an extract from butterfly beans.   A beauty food and drink characterized by blending an extract from butterfly beans.