KR102059008B1 - Cosmetic composition having skin whitening effect - Google Patents

Abstract

The present invention relates to a cosmetic composition having a skin whitening function, including a mixed extract extracted from a mixture of Woodsia glabella R. Br. ex Richards, Limosella aquatica L., Tephroseris flammea (Turcz.) Holub, and Iris koreana Nakai. According to the present invention, a skin whitening effect is significantly improved as compared to that of existing cosmetic compositions for skin whitening of natural plant materials. Also, the skin whitening effect is maintained for a long time even under exposure to sunlight such as ultraviolet rays.

Description

Cosmetic composition having skin whitening function {COSMETIC COMPOSITION HAVING SKIN WHITENING EFFECT}

The present invention relates to a cosmetic composition having a skin whitening function, and more particularly to a cosmetic composition having an excellent skin whitening effect by using a mixed extract extracted from a mixture of baby fern, canopy, hyssop and yellow irises. .

Human skin tends to appear dull and sensitive as time passes due to various environmental changes and stresses, and the skin color becomes darker.

In particular, light tones of skin symbolize health and youth, and people's interest in light tones is gradually increasing, such as using various cosmetics or skin treatments to have white and clean skin regardless of age or gender.

Therefore, a method of applying various cosmetic compositions to the skin, such as using a skin whitening agent, to have a light-toned skin as such, is commonly used, and research on the cosmetic composition for skin whitening has also been actively conducted.

Meanwhile, Patent Literature 1 below discloses a cosmetic composition for improving skin wrinkles, which is prepared by sequentially extracting cheonsun and leaves with ethanol and then sequentially dividing n-hexane, ethyl acetate and n-butanol. However, such a cosmetic composition has a problem that the skin whitening effect is short and the skin whitening effect is easily disappeared when exposed to external ultraviolet rays.

Therefore, the skin whitening effect is remarkably improved as compared to the existing cosmetic composition for skin whitening of natural plant material, and the development of a new skin whitening cosmetic composition that maintains the skin whitening effect for a long time even when exposed to ultraviolet rays such as sunlight is urgently required. to be.

Patent Document 1: Republic of Korea Patent Publication No. 10-1042718 (2011.06.13)

Therefore, in the present invention, in order to solve the above problems, the baby fern ( Woodsia glabella R. Br. Ex Richards ), Liliumella aquatica L. , the mountain bat ( Teproseris flammea (Turcz.) Holub ) and yellow iris ( Iris koreana) It was discovered by chance that the mixed extract extracted from the mixture of Nakai ) can produce a novel cosmetic composition with excellent skin whitening effect and long-lasting skin whitening function, the present invention was completed based on this.

Cosmetic composition having a skin lightening function according to an embodiment of the present invention is a baby fern ( Woodsia glabella R. Br. Ex Richards ), Liliumella aquatica L. , Tephroseris flammea (Turcz.) Holub ) And yellow iris ( Iris koreana Nakai ), characterized in that it contains a mixed extract extracted from the mixture.

In the cosmetic composition having a skin whitening function according to an embodiment of the present invention, the mixture is 50 to 100 parts by weight of Woodsia glabella R. Br. Ex Richards (Limosella aquatica L.) 50 to 150 parts by weight, 50 to 150 parts by weight of Tephroseris flammea (Turcz.) Holub and 100 to 150 parts by weight of yellow iris (Iris koreana Nakai) are mixed.

In the cosmetic composition having a skin whitening function according to an embodiment of the present invention, the mixed extract is characterized in that it is extracted from the mixture by fermentation extraction method, ultra-high pressure extraction method or after ultra-high pressure extraction method.

In the cosmetic composition having a skin whitening function according to an embodiment of the present invention, the mixed extract is characterized in that it contains 0.001 to 30% by weight based on the total weight of the cosmetic.

In the cosmetic composition having a skin whitening function according to an embodiment of the present invention, the cosmetic composition is characterized in that it has a cream, softening (skin), nutrient cosmetics (milk lotion), the formulation of the pack.

The cosmetic composition having a skin whitening function according to the present invention has a remarkably improved skin whitening effect as compared to the conventional cosmetic composition for skin whitening of natural plant materials, and has an effect of maintaining the skin whitening effect for a long time even when exposed to ultraviolet rays such as sunlight.

Before describing the invention in more detail, the terms or words used in the specification and claims are not to be limited to their ordinary or dictionary meanings, and the concept of terms is appropriately described to best explain the invention. It should be interpreted as meanings and concepts in accordance with the technical spirit of the present invention based on the principle that it can be defined. Therefore, the configuration of the embodiments described herein is only one preferred example of the present invention, and does not represent all of the technical idea of the present invention, various equivalents and modifications that can replace them at the time of the present application It should be understood that there may be

Hereinafter, preferred embodiments of the present invention will be described in detail so that those skilled in the art may easily implement the present invention.

The present invention relates to a cosmetic composition having a skin whitening function, and more particularly to a cosmetic composition having an excellent skin whitening effect by using a mixed extract extracted from a mixture of baby fern, canopy, hyssop and yellow irises. .

The cosmetic composition having a skin lightening function according to the present invention is a baby fern ( Woodsia glabella R. Br. Ex Richards ), Liliumella aquatica L. , Tephroseris flammea (Turcz.) Holub and yellow iris ( Iris koreana Nakai ) characterized in that it contains a mixed extract extracted from the mixture.

The mixed extract contained in the present invention may preferably be contained in 0.001 to 30% by weight, more preferably 0.001 to 20% by weight, most preferably 0.01 to 10% by weight relative to the total weight of the cosmetic composition Can be. When the content of the extract is less than 0.001% by weight, the skin whitening effect is weak, and when the content of the extract is more than 30% by weight, the effect of increasing the content is insignificant, and there is a problem in the safety and stability of the formulation. Not desirable

The mixed extract contained in the cosmetic composition of the present invention is, for example, 50 to 150 parts by weight of Limosella aquatica L. based on 100 parts by weight of woodsia glabella R. Br. Ex Richards, cotton wool bat (Tephroseris flammea (Turcz.) Holub) 50 to 150 parts by weight and yellow iris (Iris koreana Nakai) can be prepared through the extraction process using a mixture of 100 to 150 parts by weight.

The mixed extract used in the present invention is carried out by using the stem of the baby fern, rapeseed grass, mountain bat and yellow iris.

In addition, the baby seedling fern ( Woodsia glabella R. Br. Ex Richards ), Liliumella aquatica L. , Tephroseris flammea (Turcz.) Holub and yellow used in the preparation of the mixed extract according to the present invention The iris ( Iris koreana Nakai ) takes each stem and cleans it, and then steams it for more than 20 minutes with hot steam at 100 ℃ or higher before extraction to completely remove the potential microtoxicity.

The mixed extract used in the present invention may be extracted through a pulverization extraction method, and the pulverization extraction may be performed by pulverizing the baby fern, rapeseed grass, harpoon bat and yellow iris stem with a fine pulverizer and filtering the dust through a sieve. Can be.

At this time, it is preferable to use the pulverized pulverized product which filtered through the sieve of 300 mesh or less.

In addition, the mixed extract used in the present invention may be extracted by a solvent extraction method, the solvent used in the solvent extraction method is, for example, water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (for example, methanol, ethanol , Propanol and butanol), propylene glycol, 1.3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, chloroform, hexane and mixtures thereof may be used. .

In addition, the mixed extract used in the present invention may be extracted by the fermentation extraction method. In the fermentation extraction method used in the present invention, the extraction is carried out through the process of fermenting the baby fern, Daffodil, Sansom bat and yellow iris stem.

The fermentation extraction process is a bacterium selected from the group consisting of the baby fern, canopy grass, harpoon bat and yellow iris stem from yeast, lactic acid bacteria, Bacillus bacteria and mixtures thereof, pH 5-7 under temperature conditions of 20-40 ℃ It can be carried out through the process of extracting by fermentation for 1 to 7 days while maintaining, and can be carried out using a yeast ( Saccharomyces cerevisisae ).

In addition, the mixed extract used in the present invention may be extracted by an ultrahigh pressure extraction method. Ultra-high pressure extraction can be carried out through a process for 1 to 24 hours at a temperature of 40 to 90 ℃ at a pressure of 100 to 3,000 MPa, preferably at a temperature of 40 to 60 ℃ at a pressure of 500 to 1,000 MPa The treatment may be carried out for 12 to 24 hours, most preferably for 24 hours at a temperature of 50 ° C. at a pressure of 1,000 MPa.

In addition, the mixed extract of the present invention may be extracted by applying the above-described extraction method by mixing. For example, the mixed extract of the present invention is a fungus selected from the group consisting of finely crushed baby fern, canopy grass, harpoon bat and yellow iris stem, yeast, lactic acid bacteria, Bacillus bacteria, and a crowd consisting of other mixtures It is possible to prepare by subjecting the step of ultrahigh pressure extraction for 1 to 24 hours at a temperature of 40 to 90 ° C. at a pressure of 100 to 3,000 MPa after fermentation.

In addition, the mixed extract of the present invention may be subjected to a process such as filtration, reflux extraction of organic solvents, reduced pressure concentration, lyophilization, etc. to adjust the concentration of the extract extracted by the extraction method and increase the concentration of the active ingredient. The extract obtained by the filtration, purification and concentration of the extract by the extraction method described above will also be construed as being included in the present invention.

According to a preferred embodiment of the present invention, the mixed extract of the present invention can be prepared by the following method.

First, the baby water fern, canopy grass, wild cotton bat and yellow iris stems are sufficiently washed and pulverized, steamed with high temperature steam, and then fine filtrate is passed through a 300 mesh sieve.

The fine filtrate thus passed through the sieve is added to the yeast strain culture at a concentration of 100 g per liter. It can be cultured by adding 1 to 4% of glucose (preferably 1 to 2%) as a carbon source and 0.1 to 1% (preferably 0.2 to 0.5%) of peptone as a nitrogen source. The culture is incubated for 1 to 7 days using a 5 liter fermenter while maintaining the pH of 5-7 under a temperature condition of 20 to 40 ℃.

After the culture, the culture medium is first removed by centrifugation, and the culture medium from which the culture medium is removed is extracted by ultra-high pressure treatment for 1 to 24 hours at a temperature of 40 to 90 ° C. at a pressure of 100 to 3,000 MPa, preferably 500 The treatment is carried out at a temperature of 40 to 60 ° C. at a pressure of from 1,000 MPa for 12 to 24 hours, most preferably at a temperature of 50 ° C. at a pressure of 1,000 MPa for 24 hours.

The ultra-high pressure mixed extract was refluxed three times for 5 hours by adding ethanol to a final 70% (V / V) ethanol aqueous solution, cooled, and then filtered through Whatman # 2 filter paper.

When the filtration is completed in the present invention, the extract obtained in the extraction step is transferred to a concentration tank, concentrated under reduced pressure and lyophilized. Concentration under reduced pressure can be carried out using a rotary decompression concentrator. Concentration conditions are reduced pressure for 2 hours while putting 700 ml of extract in a 1,000 ml round flask and rotating at 50 to 70 ° C. and 600 to 1,200 rpm at a pressure of 650 mmHg to 750 mmHg. Concentrate. The concentrated solution concentrated under reduced pressure was subjected to prefreezing to lower the temperature to -40 ° C. and to keep it at -40 ° C. for 2 to 4 hours. Then, the trap was lowered to -80 ° C. through a trap refrigeration, and the vacuum was maintained at 10 to 30 m Tor. Raise again to minus 40 ° C over the next 120 minutes.

After that, it is maintained at 240 ° C. at 20 ° C., 40 minutes at 0 ° C., 340 minutes at 15 ° C., and 130 minutes at 30 ° C., and maintained at 1,300 ° C. until complete drying.

Components included in the cosmetic composition of the present invention may include, in addition to the active ingredient as an active ingredient, conventional adjuvants or carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavorings.

Cosmetic compositions of the present invention can be formulated into solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays, and the like. have.

More specifically, the cosmetic composition of the present invention may be prepared in the form of a cream, soft cosmetic (skin), nutrient cosmetic (milk lotion) or a pack, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, It may be prepared in a formulation such as cleansing water, spray or powder.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are only for illustrating the present invention more specifically, and the scope of the present invention is not limited by these examples.

Preparation Example 1 Preparation of Solvent Mixture Extract of Baby Water Fern, Daffodil, Sansommog and Yellow Iris Stem

500g of baby fern stem, 500g of canopy stem, 500g of cotton beetle stem and 500g of yellow iris stem were selected as a sample and thoroughly washed, and then steamed sufficiently with hot steam for 30 minutes. Thereafter, these were ground and passed through a 300 mesh sieve to produce a finely ground product. Ethanol was added to the final concentration to 70% (V / V) ethanol aqueous solution, refluxed three times for 5 hours, and then cooled and filtered with Whatman # 2 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized. This powder was melted to 2% (W / V) using 50% glycerin to prepare solvent extracts of baby fern, canopy, hyssop and yellow iris stem.

Preparation Example 2 Preparation of Fermented Mixture Extract of Baby Water Fern, Daffodil, Harp and Yellow Iris

500g of baby fern stems, 500g of lattices stems, 500g of cotton beetle stems and 500g of yellow iris stems were selected as a sample, thoroughly washed, and then steamed sufficiently with hot steam for 30 minutes. The mill was then finely ground to pass through a 300 mesh sieve. The micronized as described above was added to the yeast strain culture at a rate of 10 g / l. In addition, 2% (W / V) of glucose and 0.5% (W / V) of peptone were added to the carbon source and cultured.

The culture was incubated for 5 days while maintaining the pH at 37 ° C. using a 5 L fermenter. After incubation, the culture medium was centrifuged to remove the culture medium first.

The fermentation product from which the culture was firstly removed was extracted by refluxing three times for 5 hours by adding ethanol to a final 70% (V / V) ethanol aqueous solution, and cooled, and then filtered through whatman # 2 filter paper. The filtered extract was transferred to a concentration tank, and the filtered extract was concentrated under reduced pressure and freeze-dried. This powder was melted to 2% (W / V) using 50% glycerin to prepare fermented extracts of baby fern, canopy grass, wild cotton bat and yellow iris stem.

Preparation Example 3 Preparation of Ultra-High Pressure Mixture Extract of Baby Water Fern, Daffodil, Sansommog and Yellow Iris

500g of baby fern stem, 500g of canopy stem, 500g of cotton beetle stem and 500g of yellow iris stem were selected as a sample and thoroughly washed, and then steamed sufficiently with hot steam for 30 minutes. Thereafter, the finely ground pulverized product so as to pass through the 300 mesh sieve was heated to 50 ° C. under a pressure of 1,000 MPa using a ultrahigh pressure treatment machine and treated for 24 hours, and then cooled to room temperature.

The ultra-high pressure mixed extract was extracted by refluxing three times for 5 hours by adding ethanol to a final 70% (V / V) ethanol aqueous solution, and then cooled and filtered through Whatman # 2 filter paper. After filtration was completed, the extract obtained in the extraction step was transferred to a concentration tank, and the filtered extract was concentrated under reduced pressure and freeze-dried. The lyophilisate was dissolved to 2% (W / V) using 50% glycerin to prepare ultra-high pressure extracts of baby fern, canopy, hyssop and yellow iris stem.

Preparation Example 4 Preparation of Fermentation-Ultra High Pressure Mixture Extract of Baby Water Fern, Daffodil, Harp and Yellow Iris Stalk

500g of baby fern stems, 500g of lattices stems, 500g of cotton beetle stems and 500g of yellow iris stems were selected as a sample, thoroughly washed, and then steamed sufficiently with hot steam for 30 minutes. The mill was then finely ground to pass through a 300 mesh sieve. This milled product was added to the yeast strain culture at a rate of 10 g / l. In addition, 2% (W / V) of glucose and 0.5% (W / V) of peptone were added to the carbon source and cultured. The culture was incubated for 5 days while maintaining the pH at 37 ° C. using a 5 L fermenter. After incubation, the culture medium was centrifuged to remove the culture medium first.

The fermentation product from which the culture was firstly removed was heated to 50 ° C. under a pressure of 1,000 MPa using an ultrahigh pressure treatment machine, and was ultra high pressure treated for 24 hours, and then cooled to room temperature. The ultrahigh pressure fermented product was refluxed three times for 5 hours by adding ethanol to a final 70% (V / V) ethanol aqueous solution, and then cooled and filtered through Whatman # 2 filter paper. After filtration was completed, the extract obtained in the extraction step was transferred to a concentration tank, concentrated under reduced pressure at 50 ° C. or lower, and freeze-dried. Concentration under reduced pressure was concentrated using a rotary depressurizer, 700 ml of extract was put in a 1,000 ml round flask, and concentrated under reduced pressure for 2 hours while rotating at 1,000 rpm for 60 ° C at a pressure of 700 mmHg. The concentrated solution concentrated under reduced pressure was preliminarily frozen to lower the temperature to -40 ° C, maintained at -40 ° C for 3 hours, and then cooled to -80 ° C by trap refrigeration. Raised again to minus 40 ° C. over minutes.

Thereafter, the mixture was kept at minus 20 ° C for 240 minutes, 0 ° C for 40 minutes, 15 ° C for 340 minutes, and 30 ° C for 130 minutes and 1300 minutes until completely dried. The freeze-dried was dissolved to 2% using 50% glycerin to prepare a fermentation-ultra high pressure extract of the baby fern, canopy, hyssop and yellow iris stem.

Preparation Example 5 Baby Water Fern Stem Extract

2,000 g of baby fern stems were selected as a sample, and a baby fern stem extract was prepared according to the method of Example 1.

Preparation Example 6 Rhizome Stalk Extract

2,000 g of canopy stalks were selected as a sample, and a canopy stalk extract was prepared according to the method of Example 1.

Preparation Example 7 Sansam Bat Stem Extract

2,000 g of bat bat stem was selected as a sample, according to the method of Example 1 was prepared bat bat stem extract.

Preparation Example 8 Yellow Iris Stem Extract

2,000 g of yellow iris stems were selected as a sample, and yellow iris stem extracts were prepared according to the method of Example 1.

Preparation Example 9 Preparation of Solvent Mixture Extract of Rhizophyllum, Yellow Cotton Flour and Yellow Iris Stalk (Without Baby Stingray Stem)

700 g of ragweed grass stem, 700 g of cotton beetle stem and 600 g of yellow iris stem were screened and thoroughly washed, and then steamed sufficiently with hot steam for 30 minutes. Thereafter, these were ground and passed through a 300 mesh sieve to produce a finely ground product. Ethanol was added to the final concentration to 70% (V / V) ethanol aqueous solution, refluxed three times for 5 hours, and then cooled and filtered with Whatman # 2 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized. This powder was dissolved to 2% (W / V) using 50% glycerin to prepare solvent extracts of the rapeseed, grass, and yellow iris stems.

Preparation Example 10 Preparation of Solvent Mixture Extract of Baby Water Fern, Yellow Cotton Ball, and Yellow Iris Stalk

700g of baby fern stalk, 700g of cotton beetle stem and 600g of yellow iris stem were screened and thoroughly washed, and then steamed with hot steam for 30 minutes. Thereafter, these were ground and passed through a 300 mesh sieve to produce a finely ground product. Ethanol was added to the final concentration to 70% (V / V) ethanol aqueous solution, refluxed three times for 5 hours, and then cooled and filtered with Whatman # 2 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized. This powder was dissolved to 2% (W / V) using 50% glycerin to prepare a solvent extract of the baby fern, harp and yellow iris stem.

Preparation Example 11 Preparation of Solvent Mixture Extract of Baby Cub, Canopy Fragrance, and Yellow Iris Stalk

700g of baby fern stalks, 700g of canopy stalks and 600g of yellow iris stems were selected as a sample and thoroughly washed, and then steamed sufficiently with hot steam for 30 minutes. Thereafter, these were ground and passed through a 300 mesh sieve to produce a finely ground product. Ethanol was added to the final concentration to 70% (V / V) ethanol aqueous solution, refluxed three times for 5 hours, and then cooled and filtered with Whatman # 2 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized. This powder was dissolved to 2% (W / V) using 50% glycerin to prepare solvent extracts of baby fern, canopy and yellow iris stem.

Preparation Example 12 Preparation of Solvent Mixture Extract of Baby Water Fern, Canopy Pond, and Cotton Wool Stem (not including Yellow Iris Stem)

700g of baby fern stalks, 700g of canopy stalks, and 600g of cotton wool batters were screened and thoroughly washed, and then steamed sufficiently with hot steam for 30 minutes. Thereafter, these were ground and passed through a 300 mesh sieve to produce a finely ground product. Ethanol was added to the final concentration to 70% (V / V) ethanol aqueous solution, refluxed three times for 5 hours, and then cooled and filtered with Whatman # 2 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized. This powder was melted to 2% (W / V) using 50% glycerin to prepare a solvent extract of the baby fern, canopy grass, and wild boar stem.

Experimental Example 1 Intracellular Melanin Inhibitory Effect

B16F1 melanoma cells were provided by the Korea Cell Line Bank and cultured. B16F1 melanoma cells are cell lines producing melanin and are generally used for whitening screening. The medium for culturing the cells was sterile by adding DMEM, 10% FBS, 1% penicillin / streptomycin. Cell culture was incubated in an incubator at 37 ℃, 5% CO2. Melanoma cells were cultured and passaged when the 100 mm Petri dishes were about 90% full. In order to measure the whitening effect of the cosmetic composition according to the present invention, the amount of melanin produced by treating B16F1 melanoma cells was measured. Cells were seeded on 6-well plates and the extracts of Preparation Examples 1 to 12 were treated at 200 μg / ml, respectively, and then cultured for 2 days to recover the cells. The recovered cells were added with 1N NaOH containing 10% DMSO, reacted at 90 ° C. for 1 hour to be sufficiently dissolved in melanin, and the absorbance was measured at a wavelength of 405 nm using an ELISA reader. Inhibition was calculated using the following Equation 1, and the results are shown in Table 1.

[Equation 1]

Melanin Formation (%) = Treatment Absorbance / Untreated Absorbance × 100

division Melanin production (%) Untreated 100 Preparation Example 1 20.2 Preparation Example 2 20.6 Preparation Example 3 21.3 Preparation Example 4 14.2 Preparation Example 5 60.5 Preparation Example 6 59.7 Preparation Example 7 61.4 Preparation Example 8 62.5 Preparation Example 9 42.5 Preparation Example 10 40.8 Preparation Example 11 44.6 Preparation Example 12 45.7

Looking at the results of Table 1, it can be seen that in the case of Preparation Examples 1 to 4 of the mixed extract of the present invention, melanin production was significantly lower than in Preparation Examples 5 to 12.

In particular, in the case of Example 4 containing the extract performed both fermentation and ultra-high pressure extract can be confirmed that the melanin production is the lowest.

On the other hand, when comparing the melanin production of Preparation Examples 1 to 4 and Preparation Examples 9 to 12 in Table 1, the mixed extract was prepared using a mixture of the baby fern, rapeseed, sansom bat and yellow iris stem In one case it can be seen that the melanin production is significantly lower than the mixed extract prepared using any one of the four components excluded.

On the other hand, when comparing the Preparation Examples 5 to 8 and Preparation Examples 1 to 4 or Preparation Examples 9 to 12 in Table 1, the extract was prepared by using a baby fern, Daffodil, Sansom bat or yellow iris stem alone. In one case, it can be seen that the melanin production lowering effect is insignificant compared to the case where these are mixed.

As a result, when the results of Table 1 are summarized, the whitening effect was remarkable in the case of Preparation Examples 1 to 4, which prepared mixed extracts using a mixture of baby fern, canopy, harp and yellow iris stem. It is excellent, especially in the case of Example 4 containing the extract performed both fermentation and ultra-high pressure extract as the extraction method can be seen that the lowest melanin production.

Cosmetics Manufacturing

Cream type cosmetics containing the mixed extract of Preparation Examples 1 to 4 and the extract of Preparation Examples 5 to 12 were prepared as shown in Tables 2 and 3 below (Formulation Example: Cream).

Examples 1 to 4 show examples of the formulation of the cosmetic composition containing the mixed extract of Preparation Examples 1 to 4, respectively, and Comparative Examples 2 to 9 used the extracts of Preparation Examples 5 to 12 as active ingredients, respectively. The formulation example of the cosmetic composition of this is shown. Comparative Example 1 shows a formulation example of a cosmetic composition containing no extract of Preparation Examples 1 to 12.

ingredient
(Unit: weight%) practice
Example 1 practice
Example 2 practice
Example 3 practice
Example 4 compare
Example 1 compare
Example 2 compare
Example 3 compare
Example 4 compare
Example 5 Glyceryl
Stearate SE 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Stearyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 lanolin 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Polysorbate 60 1.3 1.3 1.3 1.3 1.3 1.3 1.3 1.3 1.3 Sorbitan
Stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Cured Vegetable Oil 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Mineral oil 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Squalane 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Trioctanoine 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Tocopherol Acetate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Carboxy vinyl
Polymer 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 glycerin 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 1.3-butylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Sodium
Hyaluronate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Triethanolamine 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 0.12 Methylparaben 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Spices 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Preparation Example 1 Extract 3 0 0 0 0 0 0 0 0 Preparation Example 2 Extract 0 3 0 0 0 0 0 0 0 Preparation Example 3 Extract 0 0 3 0 0 0 0 0 0 Preparation Example 4 Extract 0 0 0 3 0 0 0 0 0 Preparation Example 5 Extract 0 0 0 0 0 3 0 0 0 Preparation Example 6 Extract 0 0 0 0 0 0 3 0 0 Preparation Example 7 Extract 0 0 0 0 0 0 0 3 0 Preparation Example 8 Extract 0 0 0 0 0 0 0 0 3 Preparation Example 9 Extract 0 0 0 0 0 0 0 0 0 Preparation Example 10 Extract 0 0 0 0 0 0 0 0 0 Preparation 11 Extract 0 0 0 0 0 0 0 0 0 Preparation 12 Extract 0 0 0 0 0 0 0 0 0 Purified water Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount Remaining amount

ingredient
(Unit: weight%) compare
Example 6 compare
Example 7 compare
Example 8 compare
Example 9 Glyceryl
Stearate SE 1.5 1.5 1.5 1.5 Stearyl alcohol 1.5 1.5 1.5 1.5 lanolin 1.5 1.5 1.5 1.5 Polysorbate 60 1.3 1.3 1.3 1.3 Sorbitan
Stearate 0.5 0.5 0.5 0.5 Cured Vegetable Oil 1.0 1.0 1.0 1.0 Mineral oil 5.0 5.0 5.0 5.0 Squalane 3.0 3.0 3.0 3.0 Trioctanoine 2.0 2.0 2.0 2.0 Dimethicone 1.0 1.0 1.0 1.0 Tocopherol Acetate 0.5 0.5 0.5 0.5 Carboxy vinyl
Polymer 0.12 0.12 0.12 0.12 glycerin 6.0 6.0 6.0 6.0 1.3-butylene glycol 2.0 2.0 2.0 2.0 Sodium
Hyaluronate 1.0 1.0 1.0 1.0 Triethanolamine 0.12 0.12 0.12 0.12 Methylparaben 0.2 0.2 0.2 0.2 Spices 0.02 0.02 0.02 0.02 Preparation Example 1 Extract 0 0 0 0 Preparation Example 2 Extract 0 0 0 0 Preparation Example 3 Extract 0 0 0 0 Preparation Example 4 Extract 0 0 0 0 Preparation Example 5 Extract 0 0 0 0 Preparation Example 6 Extract 0 0 0 0 Preparation Example 7 Extract 0 0 0 0 Preparation Example 8 Extract 0 0 0 0 Preparation Example 9 Extract 3 0 0 0 Preparation Example 10 Extract 0 3 0 0 Preparation 11 Extract 0 0 3 0 Preparation 12 Extract 0 0 0 3 Purified water Remaining amount Remaining amount Remaining amount Remaining amount

Experimental Example 2 Skin Whitening Sustaining Effect Experiment

Experimental participants using the cosmetic creams of Examples 1 to 4 and Comparative Examples 1 to 9 of Tables 2 and 3 to test the persistence of the skin whitening effect of the cosmetic composition by the mixed extract of the present invention (10 adult male 10 Persons and 10 adult women in their 20s) were washed three times a day for 10 days and then applied with 2g of cosmetic cream on their face and the improvement of skin whitening was measured on the 10th day.

Thereafter, the cosmetic cream was not applied for 5 days from the 11th day, and the whitening degree of the skin was measured every day and the results are shown in Table 4 below.

Skin whitening was measured by setting the average value of the tone of the skin color measured after washing the face of 100 adult men and women immediately before the start of the experiment and converting the degree of lightening of the skin tone into a numerical value.

division Before experiment Day 10 Day 11 Day 12 Day 13 Day 14 Day 15 Example 1 100 145 145 142 141 140 138 Example 2 100 148 147 145 144 142 140 Example 3 100 150 150 148 145 140 138 Example 4 100 160 158 154 152 151 148 Comparative Example 1 100 115 108 105 102 100 100 Comparative Example 2 100 120 117 115 107 105 103 Comparative Example 3 100 121 115 113 105 102 100 Comparative Example 4 100 122 117 114 102 100 100 Comparative Example 5 100 124 117 113 110 105 102 Comparative Example 6 100 131 124 124 123 118 115 Comparative Example 7 100 133 125 124 121 114 105 Comparative Example 8 100 131 124 121 115 105 100 Comparative Example 9 100 130 124 120 114 110 102

Looking at the results of Table 4, it can be confirmed once again the skin whitening effect of the cosmetic composition according to the present invention Examples 1 to 4, in particular when comparing the Examples 1 to 4 with Comparative Examples 1 to 9, The effect is also very good.

Experimental Example 3 skin irritation test 1

Experiments were carried out using four New Zealand male rabbits weighing between 1.75 and 2.25 kg. Before applying the mixed extract of the present invention as a test substance, the rabbit's dorsal side was depilated, and the epidermis was rubbed using two needles on each side of the rabbit. Each extract was dissolved in distilled water at a concentration of 50 mg / ml, and 0.5 ml of abraded part and intact part were respectively administered, and 0.5 cm of distilled water was administered to the non-treated part, followed by 2 cm gauze. Covered with After application, the treatment and non-treatment tools were fixed well with non-irritating tape and the residue was removed after 24 hours. Skin reaction application time was observed after removing the patch after 24 hours and 72 hours, the results are shown in Table 5 below.

division First aid Non-treatment change Erythema and skin edema Erythema and skin edema part With the mirror Abrasion With the mirror Abrasion With the mirror Abrasion With the mirror Abrasion time 24 72 24 72 24 72 24 72 24 72 24 72 24 72 24 72 Preparation Example 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Preparation Example 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Preparation Example 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Preparation Example 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 all 0 0

No animals died during the experiment, body weight gained and no obvious response to stimulation. After testing the mixed extract of the present invention in rabbits, the applied skin was dyed pale brown but was not affected by skin reaction experiments. As can be seen from Table 5, no irritation was observed in both the treated and untreated skins, and no erythema, edema, or eschar appeared. According to the results of Table 5, the mixed extract of the present invention can be confirmed that it does not give any irritation to the skin.

Experimental Example 4 Skin Stimulation Test 2

Experiments were carried out using four New Zealand male rabbits weighing between 1.75 and 2.25 kg. Before applying the cosmetic composition of Examples 1 to 4 of the present invention as a test substance, the rabbit's dorsal side was epilated, and the epidermis was rubbed using the needle on each of the two sides of the rabbit. Each extract was dissolved in distilled water at a concentration of 50 mg / ml, and 0.5 ml of abraded part and intact part were respectively administered, and 0.5 cm of distilled water was administered to the non-treated part, followed by 2 cm gauze. Covered with After application, the treatment and non-treatment tools were fixed well with non-irritating tape and the residue was removed after 24 hours. Skin reaction application time was observed after removing the patch after 24 hours and 72 hours, the results are shown in Table 6 below.

division First aid Non-treatment change Erythema and skin edema Erythema and skin edema part With the mirror Abrasion With the mirror Abrasion With the mirror Abrasion With the mirror Abrasion time 24 72 24 72 24 72 24 72 24 72 24 72 24 72 24 72 Example 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Example 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Example 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Example 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 all 0 0

No animals died during the experiment, body weight gained and no obvious response to stimulation. After testing the mixed extract of the present invention in rabbits, the applied skin was dyed pale brown but was not affected by skin reaction experiments. As can be seen from Table 6, no irritation was observed in both the treated and untreated skins, and no erythema, edema, or eschar appeared. According to the results of Table 6, it can be seen that the mixed extract of the present invention does not cause any irritation to the skin.

Experimental Example 5 Eye Mud Stimulation Experiment 1

Experiments were carried out using four New Zealand male rabbits weighing between 1.75 and 2.25 kg. After each extract was dissolved in distilled water at a concentration of 50 mg / ml, each experimental animal was treated with 0.1 ml in the conjunctival sac of the left eye as a treatment and 0.1 ml of distilled water in the conjunctival sac of the right eye as an untreated control. Reaction experiments on eye toxicity were observed 1, 2, 3, 4, 7 days after the administration of the test substance, the results are shown in Table 7 below.

division
group
Day after kill (day) One 2 3 4 7
Preparation Example 1
cornea 0 0 0 0 0 Iris 0 0 0 0 0 conjunctiva 0 0 0 0 0
Preparation Example 2
cornea 0 0 0 0 0 Iris 0 0 0 0 0 conjunctiva 0 0 0 0 0
Preparation Example 3
cornea 0 0 0 0 0 Iris 0 0 0 0 0 conjunctiva 0 0 0 0 0
Preparation Example 4
cornea 0 0 0 0 0 Iris 0 0 0 0 0 conjunctiva 0 0 0 0 0 Untreated control 0 0 0 0 0 all 0

Ocular mucosal irritation in rabbits showed that all rabbits were normal. There were no pathological changes such as tearing (nursing), redness, edema and pus during the 7 day test period. According to the results of Table 7, the mixed extract of the present invention was found to have no irritation to the eye mucosa.

Experimental Example 6 Eye Mud Stimulation Experiment 2

Experiments were carried out using four New Zealand male rabbits weighing between 1.75 and 2.25 kg. After dissolving the cosmetic composition of Examples 1 to 4 of the present invention in distilled water at a concentration of 50 mg / ml, each experimental animal was treated with 0.1 ml in the conjunctival sac of the left eye as a treatment, and in the conjunctival sac of the right eye as an untreated control. 0.1 ml of distilled water was instilled. Reaction experiments on eye toxicity were observed 1, 2, 3, 4, 7 days after the administration of the test substance, the results are shown in Table 8.

division
group
Day after kill (day) One 2 3 4 7
Example 1
cornea 0 0 0 0 0 Iris 0 0 0 0 0 conjunctiva 0 0 0 0 0
Example 2
cornea 0 0 0 0 0 Iris 0 0 0 0 0 conjunctiva 0 0 0 0 0
Example 3
cornea 0 0 0 0 0 Iris 0 0 0 0 0 conjunctiva 0 0 0 0 0
Example 4
cornea 0 0 0 0 0 Iris 0 0 0 0 0 conjunctiva 0 0 0 0 0 Untreated control 0 0 0 0 0 all 0

Ocular mucosal irritation in rabbits showed that all rabbits were normal. There were no pathological changes such as tearing (nursing), redness, edema and pus during the 7 day test period. According to the results of Table 8, the mixed extract of the present invention was found to have no irritation to the eye mucosa.

Claims (5)

A mixed extract extracted from a mixture of Babysia fern ( Woodsia glabella R. Br. Ex Richards ), Liliumella aquatica L. , Teal ( Phroseris flammea (Turcz.) Holub ) and Yellow iris ( Iris koreana Nakai ) Cosmetic composition having a skin whitening function containing. The method according to claim 1,
The mixture is 50 to 150 parts by weight of Limosella aquatica L., Tephroseris flammea (Turcz.) Holub, based on 100 parts by weight of Woodsia glabella R. Br. Ex Richards. Weight part and yellow iris (Iris koreana Nakai) 100 to 150 parts by weight of a cosmetic composition having a skin whitening function. The method according to claim 1 or 2,
The mixed extract is a cosmetic composition having a skin whitening function that is extracted from the mixture using a fermentation extraction method, ultra high pressure extraction method or an ultra high pressure extraction method after fermentation. The method according to claim 1 or 2,
The mixed extract is a cosmetic composition having a skin whitening function that is contained in 0.001 to 30% by weight relative to the total weight of the cosmetic. The method according to claim 1 or 2,
The cosmetic composition is a cosmetic composition having a skin whitening function having a formulation of any one of a cream, a skin and a pack.