KR101960757B1 - Cosmetic composition having lipolysis effect - Google Patents

Abstract

The lipid-decomposing cosmetic composition according to one embodiment of the present invention contains as an active ingredient, a mixed extract consisting of an egg white extract, a peony extract, a clove extract, a golden extract, and an ointment extract. The cosmetic composition having lipolytic effect according to the present invention inhibits lipid synthesis and increases the amount of fat decomposition by using an extract obtained from a mixture of the extracts of white flour, peony extract, clove extract, golden extract and fragrance extract, There is an effect of reducing the subcutaneous fat layer by decomposing the fat.

Description

COSMETIC COMPOSITION HAVING LIPOLYSIS EFFECT [0002]

The present invention relates to a cosmetic composition having lipolytic activity, and more particularly, to a cosmetic composition having a lipolytic activity, and more particularly, to a cosmetic composition having lipolytic activity, which comprises, as an active ingredient, a mixed extract consisting of a white papaya extract, The present invention relates to a cosmetic composition having lipolytic effect which can be decomposed.

Skin is the most important organ in the human body that is in close contact with the external environment and protects the inside of the human body. This skin is divided into epidermis, dermis and hypodermis.

Here, the subcutaneous fat is composed of adipocytes, which constitute adipose tissue that stores energy as fat, and plays a role of accumulating or releasing energy in vivo. In other words, subcutaneous fat is stored in the adipocytes as triglyceride when the supply is higher than the demand for energy, and then decomposed into free fatty acids and glucose when the energy is depleted.

In recent years, in the modern society, due to the economic growth, the obesity population due to the lack of exercise and the high-protein diet is rapidly increasing, and many diseases are caused by obesity. Accordingly, in the modern society, exercise, diet, and medication are being performed to treat obesity.

However, modern people who are busy in life can not expect appropriate effects to solve obesity and obesity diseases through exercise therapy and diet, and moreover, fat cells can be reduced in size once they are made, There is a problem that remains in the human body permanently. Accordingly, development of a safe external skin preparation (for example, ointment, cosmetics, etc.) capable of helping lipolysis can be said to be appropriate.

Recently, consumers are increasingly interested in natural cosmetics and many cosmetics using herbal ingredients are being released. Cosmetics using herbal materials have been studied as plant materials having an effect directly on the skin, but most of them have been specialized in lipid-degrading skin external preparations or cosmetics utilizing a single natural product or a mixed extract of natural products.

Accordingly, there is a need for a lipid-degrading skin external preparation or cosmetic which is capable of degrading fat remaining in adipocytes and being harmless to the human body.

Korean Patent Publication No. 10-2007-0025413 Korean Patent Laid-Open No. 10-2012-0120736

It is an object of the present invention to provide a cosmetic composition which is safe to the human body and has few side effects but can inhibit fat synthesis, increase the amount of fat decomposition and decompose triglyceride of fat cells, thereby reducing the subcutaneous fat layer.

The problems to be solved by the present invention are not limited to the above-mentioned problems, and other matters not mentioned can be clearly understood by those skilled in the art from the following description.

The above object is achieved by a cosmetic composition having lipolytic activity which comprises, as an active ingredient, a mixed extract consisting of an egg white extract, a peony extract, a clove extract, a golden extract and an ointment extract according to the present invention.

The above mixed extract is prepared by mixing the above white flour extracts: the above peony extracts: the above clove extracts: the above golden extracts: the fragrance extracts at a weight ratio of 0.9-1.1: 0.9-1.1: 0.9-1.1: 0.9-1.1: 0.9-1.1 .

In the mixed extract, the whitehead extract, the peony extract, the clove extract, the golden extract, and the fragrance extract may be mixed at the same weight ratio.

The mixed extract may be contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition.

The white back dow extract, the peony root extract, the clove extract, the golden extract and the fragrant extract were prepared by pulverizing each sample of white back, peony, clove, golden, and ointment, immersing it in an extraction solvent, It can be provided as an image.

The extraction solvent is provided as an aqueous solution of ethanol in an amount of 5 to 15 times by weight with respect to each sample, and the deposition temperature and the deposition time with respect to the extraction solvent may be in the range of 40 to 70 ° C and 2 to 7 hours, respectively.

The product obtained by immersing in the extraction solvent and hot-extracted is allowed to stand for 1 to 3 days, filtered through a filter paper having a permeation size of 100 μm or less, concentrated under reduced pressure at 60 ° C. or lower and freeze-dried through a freeze dryer.

The above-mentioned mixed extract can be obtained by mixing each sample of white back, peony, clove, golden, and ointment, or extracting each of the individual samples of white back, peony, clove, .

The present invention relates to a cosmetic composition comprising a mixture of extracts or complex extracts comprising the extracts of Leptopodiaceae, Peony Extract, Clove Extract, Golden Extract and Flavor Extract to the cosmetic composition to inhibit lipid synthesis and increase the amount of decomposed fat, Can reduce the subcutaneous fat layer. In particular, it exerts synergistic effects superior to the single extracts of each of the backbone, the peony, the clove, the golden and the fragrance.

Further, the present invention is advantageous in that it does not show cytotoxicity when applied to skin and has little side effects by applying a natural extract extracted from a natural substance of white clover, peony, clove, golden and perfume safe to human body to a cosmetic composition.

FIG. 1 is a graph showing the cell survival rate of 3T3-L1 cells as a mixed extract in cosmetic composition having lipolytic effect according to an embodiment of the present invention.
FIG. 2 is a graph showing the content of triglyceride (TG) in 3T3-L1 cells in a mixed extract treatment in a cosmetic composition having lipolytic activity according to an embodiment of the present invention.
FIG. 3 is a graph showing the degree of accumulation of fat formed in 3T3-L1 cells in a mixed extract treatment in a cosmetic composition having lipolytic effect according to an embodiment of the present invention.

Hereinafter, a cosmetic composition having lipolytic activity according to an embodiment of the present invention (hereinafter referred to as 'cosmetic composition') will be described in detail. In the following description, well-known functions or constructions are not described in order to avoid unnecessary obscuration of the present invention.

Firstly, the Dolichos Lablab used in the cosmetic composition of the present invention is a so-called ripe seed called bean, soya bean, soybean, soybean, and soybean. These backpacks remove the narrative, remove the moisture, relax the middle chin, lower the energy, and warm the stomach. It is also used to effectively reduce the accumulation of triglycerides in hepatocellular carcinoma cells (HepG2) in nonalcoholic fatty liver disease (NAFLD).

Paeonia Lactiflora is a perennial herb that belongs to the family paeoniaceae. It is native to some parts of China and Korea, and the herbal medicines and herbal medicines refer to the roots of Paeonia lactiflora or other herbaceous plants. Peonies contain monoterpene glycoside, acidic polysaccharide, tannin, etc., and have a sedative action, platelet restraining action, action on purifying system, antitumor action, immunity Enhancing action, and anti-inflammatory action. In addition, peonies strongly inhibit alpha-glucosidase and have an effect of lowering blood glucose and increasing insulin secretion.

Syzygium Aromaticum is a plated sheep and plant which contains eugenol, acetyl eugenol, chavicol, eugenol salcylate, beta-caryophyllene, And contains humulene as a main component. These cloves are used for gastric cancer, stomach cancer, stomach cramps, increased digestive and sexual function, gum inflammation and gum ache. Pharmacological action promotes gastric secretion, analgesia, spasms, Antiparasitic, and hypotensive effects. In particular, clove is a composition for the prevention and treatment of metabolic syndrome, and is used for preventing or treating metabolic syndrome which exhibits weight loss, anti-inflammatory activity hypo-hemicidal activity and exhibits diabetes, obesity, arteriosclerosis and hyperlipemia.

The golden (Scutellaria Baicalensis) is the root of the blooming grass, which means it has been removed as it is. Gold has little odor and bitter taste. Gold is useful for the treatment of obesity and the treatment of diabetes through inhibition of fat accumulation by promoting antidiabetic effect and flavor obesity effect.

The anti-algae (Elsholtzia Ciliata) is also called anger, yajo, yaya ore, and it is a year-old herb that gives a strong fragrance to the body. Herbal oil is used as a medicinal material on the ground including the fruit. Perfume contains about 1% essential oil, which is Elscholtziaketon and also Sesquiterpen. This perfume is effective for fever, sweating, and fever, and it comforts the stomach, so it is used to treat cold and chills, headache, abdominal pain, vomiting and diarrhea. It is also used as a remedy for sweating, swelling of the body, swelling, swelling, etc. Furthermore, anti-oils are also known as herbal medicines that inhibit fat accumulation and are thus used for the treatment of obesity.

The cosmetic composition according to one embodiment of the present invention includes a mixed extract of each of the extracts obtained from the above-mentioned backbone, peony, clove, golden and ointment as effective ingredients.

In this case, each of the white back dow extract, peony extract, clove extract, golden extract, and fennel mixed extract is prepared by pulverizing each sample of white back, peony, clove, do. For reference, the sample refers to the roots of dried shade, white mulberry, peony, clove, golden and ointment.

First, each of the roots of the backbone, the peony, the clove, the golden and the perfume are washed and dried. In other words, the roots of backbone, peony, clove, and ointment are cleaned with water and then dried in the shade for a certain period of time.

Then, each dried piece of white, brown, peach, clove, golden and perfume is placed in an extraction container and extracted at a suitable temperature for a certain time in a certain amount of extraction solvent.

At this time, depending on the solvent to be extracted, there may be differences in extraction degree and loss degree of the active ingredients of each of white back, peony, clove, golden and perfume, so it is preferable to select an appropriate solvent. For example, a solvent selected from water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, hexane or a mixed solvent thereof may be used, but the extraction solvent according to one embodiment of the present invention is used as an aqueous ethanol solution. The amount of ethanol used is 5 to 15 times by weight, preferably 10 times by weight, for each sample of white flakes, peanuts, cloves, golden and perfume.

On the other hand, the immersion temperature for the extraction solvent is maintained at 40 to 70 캜, preferably 55 캜, because it is most effective for preventing decay, discoloration and detachment. The immersion time for the extraction solvent may range from 2 to 7 hours, but it is preferable to maintain the immersion time for 4 hours. Further, for example, cold extraction, ultrasonic extraction, reflux cooling extraction, hot water extraction, room temperature extraction, or the like may be used as the extraction method, but the cosmetic composition according to one embodiment of the present invention uses hot water extraction.

Then, each of the extracts obtained by the above-mentioned immersion, that is, the extracts of white clover, peony, clove, golden and perfume are left for 1 to 3 days, preferably about 2 days.

Then the filtrate is filtered using a filtration apparatus for 2 days. At this time, the filtration is a process of removing suspended solid particles from the extract, and may be any one of ultrafiltration, freeze filtration, centrifugation, etc., by filtering out particles using cotton, nylon, paper or the like. However, each of the extracts of the white flock, the peony, the clove, the golden and the fragrance left for 2 days are filtered using a filter paper having a permeation size of 100 mu m or less. For reference, further separation steps may be carried out by various chromatographies (size, charge, hydrophobicity or affinity chromatographic).

Then, each of the extracts of the impurities-filtered white flakes, peanuts, cloves, golden and perfume are concentrated and dried at 60 ° C or lower. Then, it becomes a powder form (form) from each extract of white back, peony, clove, golden and perfume.

At this time, each of the extracts of white clover, peony, clove, golden and perfume can be dried using vacuum drying, hot air drying, spray drying, foam drying, high frequency drying, vacuum drying and freeze drying, According to the embodiment, the extracts of each of the backbone, peony, clove, golden and ointment are lyophilized by a freeze dryer. For reference, a process of pulverizing each of the final dried white flakes, peanuts, cloves, gold and fragrance may be added as needed.

Hereinafter, each of the extracts of white clover, peony root, clove, golden and ointment oil prepared through the above process will be briefly described.

Preparation of Whitehead Two Extracts

The dried and aged backbone pieces were shredded in shade, and then 500 g of the mixture was heated with hot water for 7 hours in a 70% (v / v) aqueous ethanol solution of 10 times, cooled with water, Lt; / RTI > The filtered extract was concentrated under reduced pressure at 60 DEG C or lower and completely dried using a freeze dryer to obtain 103 g of an opacity extract.

Manufacture of peony extracts

The dried peony was pulverized in shade, and then 500 g was heated with hot water for 7 hours in a 70% (v / v) ethanol aqueous solution of 10 times, cooled down, and filtered through a filter paper having a size of 100 μm. The filtered extract was concentrated under reduced pressure at 60 DEG C or lower and completely dried using a freeze dryer to obtain 165 g of a peony root extract.

Production of clove extract

After shredding the cloves dried in the shade, 500 g of the mixture was heated and extracted with 10 times of 70% (v / v) aqueous ethanol solution for 4 hours, cooled, and then filtered with a filter paper having a permeation size of 100 μm . The filtered extract was concentrated under reduced pressure at 60 DEG C or lower and completely dried using a freeze dryer to obtain 124 g of a clove extract.

Manufacture of golden extracts

After drying the shade, the dried gold was pulverized. Then, 500 g of the mixture was heated with hot water for 7 hours in a 70% (v / v) aqueous ethanol solution of 10 times, cooled with water, and filtered through a filter paper having a permeation size of 100 μm . The filtrate was concentrated under reduced pressure at 60 DEG C or lower and completely dried using a freeze dryer to obtain 88 g of a golden extract.

Preparation of ointment extract

The fragrance oil dried in the shade was pulverized, and then 500 g of the mixture was heated with hot water for 7 hours in a 70% (v / v) ethanol aqueous solution of 10 times, cooled down, and filtered through a filter paper having a permeation size of 100 μm . The filtered extract was concentrated under reduced pressure at 60 DEG C or lower and completely dried using a freeze dryer to obtain 130 g of an extract of the fragrance.

In the cosmetic composition according to one embodiment of the present invention, the mixed extract of the present invention contains 0.9-1.1: 0.9-1.1: 0.9-1.1: 0.9: 0.9, To 1.1: 0.9 to 1.1. That is, the mixed extracts composed of the egg white extract, the peony extract, the clove extract, the golden extract and the fragrance extract are mixed substantially in the same weight ratio.

In addition, if it is present in the form of a solution or a powder in the form of microcapsules, nanocapsules, nanosome or the like containing an extract of Laid-back, chamomile extract, clove extract, golden extract and fragrance extract, The amount of the solution or powder in the cosmetic composition may be adjusted.

In addition, cosmetics prepared from the cosmetic composition containing the present invention as the active ingredient of the present invention can be prepared in the form of a general emulsified formulation and a solubilized formulation. Cosmetics of the emulsified form include nutritive lotion, cream, essence and the like, and the cosmetics of the solubilized form include the flexible lotion. Furthermore, in addition to the cosmetics containing the backbone extracts, the peony extracts, the clove extracts, the golden extracts and the fragrant extracts, besides the dermatologically acceptable mediums or bases, . ≪ / RTI >

Meanwhile, the cosmetic composition according to one embodiment of the present invention may further comprise at least one selected from the group consisting of a fat substance, a solubilizer, an organic solvent, a gelling agent, a softening agent, an antioxidant, a thickening agent Stabilizers, suspending agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents and chelating agents, vitamins, preservatives, blocking agents, essential oils, dyes, wetting agents, Pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient conventionally used in cosmetics, as well as adjuvants commonly used in the cosmetics or dermatological sciences. And the above ingredients may be introduced in amounts commonly used in the dermatology field.

The cosmetic composition according to one embodiment of the present invention can be used as a skin lotion, a skin softener, a skin toner, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, , Shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders and eye shadows.

Hereinafter, the present invention will be described in more detail through experiments. The experimental methods and experimental results described below are only for illustrating the present invention, and therefore, the scope of the present invention is not construed as being limited by the experimental methods and experimental results described below.

For reference, the mixed extract according to an embodiment of the present invention may be extracted after mixing each sample of white back, peony, clove, golden, and ointment, and the white back, Each of the extracts may be mixed with each extract after obtaining each of the extracts.

In the following, experiments were performed using the mixed extracts obtained by mixing the respective extracts at substantially the same weight ratios after obtaining the white flour extract, peony root extract, clove extract, golden extract and fragrant oil extract through the above-mentioned manufacturing process.

Experimental methods and experimental results

In order to examine the effect of the cosmetic composition containing the extract as a active ingredient on the cell survival rate, 3T3-L1 fibroblast, a fibroblast, Cytotoxicity of mixed extracts in adipocytes was assessed by MTT assay. The mixed extracts were treated with confluent 3T3-L1 preadipocytes for 24 hours at different concentrations. After treatment, 2 mg / ml of MTT dissolved in PBS was diluted in DMEM medium at 200 μg / ml and further incubated in an incubator at 37 ° C for 1 hour. After the reaction was completed, the medium was completely removed and 300 mu l of DMSO was added to dissolve the violet formazan. The dissociated formazan was added to a 96-well plate in an amount of 100 μl, and the absorbance was measured at 540 nm using a microplate reader.

The cell viability was calculated (% of control) using the absorbance of untreated 3T3-L1 preadipocytes as a control and the cytotoxicity of the mixed extract was evaluated. The experiment was repeated three times, and an average value was derived according to the following formula (1).

[Equation 1]

Cell survival rate (%) = (absorbance at 540 nm in the experimental group / absorbance at 540 nm in the control) * 100

bracket Experimental group  Cytotoxicity division Cell survival rate (%) Control group 100 Each of the mixed extracts (10 ug / ml) 97.3 Each of the mixed extracts (50 ug / ml) 92.6

Referring to Table 1 and FIG. 1, it was confirmed that the cell survival rate of the mixed extract according to one embodiment of the present invention was 90% or more when compared with the control group. Therefore, when the mixed extract of the present invention was treated together, it was confirmed that there was no toxicity in adipocytes, and it was confirmed that it is a safe raw material.

Cell culture and differentiation

3T3-L1 cells, a fibroblast cell line of mice, were cultured in a 6-well culture plate containing DMEM medium containing 10% fetal bovine serum (FBS) at 1 × 10 ⁵ cells / well Lt; / RTI > After 2 days, the cells were replaced with fresh DMEM (containing 10% FBS) medium and cultured for 2 days. Then, the cultured cells were again induced to differentiate into DMEM (containing 10% FBS) containing 1 μg / ml insulin, 0.5 mM IBMX and 0.25 μM dexamethasone, and after 2 days, The cells were cultured for 5 days in DMEM supplemented with insulin. After 5 days, the medium was replaced with normal medium (DMEM, containing 10% FBS), and the mixed extract was treated and cultured for 48 hours while observing until the cells morphologically changed into adipocytes.

Triglyceride content measurement

The triglyceride assay kit was used to determine the neutral fat content of 3T3-L1 cells in order to determine whether the mixed extract inhibited the accumulation of triglycerides in the cells. 3T3-L1 cells were seeded at 7 × 10 4 cells / mL in a 6-well plate and each sample was treated with differentiation for 6 days. After 2 days of sample treatment, the cells were washed with PBS, and the cells were collected using a scraper to extract intracellular triglyceride using an ultrasonic grinder. After centrifugation (12,000 rpm, 15 min, 4 ° C), the supernatant containing the triglyceride was collected and the content of triglyceride was measured using a triglyceride kit.

division TG LEVEL Differentiated cell control 100 (50 ug / ml) 80 Peony (50 ug / ml) 69.7 Cloves (50 ug / ml) 76.8 Gold (50 ug / ml) 70.3 Ointment (50 ug / ml) 64.5 Mixed extract (50 ug / ml) 52.1 Mixed extract (50 ug / ml) 30.2

As a result of measuring the degree of reduction of intracellular triglyceride (TG) content by the mixed extract, the level of triglyceride content in the cell was decreased as the concentration of the mixed extract was increased, as shown in [Table 2] and [ , And showed a decrease in triglyceride content of about 70% compared to the control (control) at the highest sample treatment concentration of 50 / / ml. From the above results, it can be seen that the cosmetic composition reduces fat accumulation in adipocytes.

Total soil mass measurement (Oil red 0 staining)

In order to investigate the inhibition of the differentiation of 3T3-L1 cells from the mixed extracts, the total lipid amount was measured using Oil Red O staining method. 3T3-L1 cells were plated at 7 × 10 4 cells / mL in a 6-well plate, and the mixed extracts were treated with differentiated adipocytes for 48 hours. Then, the cell culture medium was removed, and the cells were washed with PBS. To fix the cells, 500 μL of 4% paraformaldehyde was added to each well and fixed at 4 ° C for 1 hour. Paraformaldehyde was then removed, washed 3 times with PBS, and 400 μL of Oil Red O dye was added. The cells were stained for 30 minutes at room temperature and washed three times with PBS. 200 μL of 2-propanol per well was added, and the dye was stained in adipocytes. The absorbance was measured at 500 nm using a microplate reader.

division Lipid accumulation (%) Differentiated cell control 100 (50 ug / ml) 70 Peony (50 ug / ml) 68 Cloves (50 ug / ml) 79.5 Gold (50 ug / ml) 71.1 Ointment (50 ug / ml) 60.5 Mixed extract (50 ug / ml) 40.5 Mixed extract (50 ug / ml) 25.8

Referring to [Table 3] and [Figure 3], it can be confirmed that the lipid content of the mixed extract decreases. Therefore, it can be confirmed that the cosmetic composition comprising the extract of Bacillus subtilis, Peony root extract, Clove extract, Golden extract and Flavor oil extract as an active ingredient shows an effect of inhibiting fat accumulation in adipocytes.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, is intended to cover various modifications and equivalent arrangements included within the spirit and scope of the appended claims. something to do. Accordingly, the true scope of the present invention should be determined by the following claims.

Claims (8)

A cosmetic composition having lipolytic activity comprising, as an active ingredient, a mixed extract consisting of an opioid extract, a peony extract, a clove extract, a golden extract and an ointment extract.
The method according to claim 1,
The above mixed extract is prepared by mixing the above white extract: the above peony extract: the above clove extract: the above golden extract: the above fragrant extract in a weight ratio of 0.9-1.1: 0.9-1.1: 0.9-1.1: 0.9-1.1: 0.9-1.1 Wherein the cosmetic composition has lipolytic activity.
The method according to claim 1,
The cosmetic composition for lipolytic activity according to claim 1 or 2, wherein the fragrance extract, the peony extract, the clove extract, the golden extract and the fragrance extract are mixed in the same weight ratio.
The method according to claim 1,
Wherein the mixed extract is contained in an amount of 0.01 to 20% by weight based on the total weight of the cosmetic composition.
The method according to claim 1,
The white back dow extract, the peony root extract, the clove extract, the golden extract and the fragrant extract were prepared by pulverizing each sample of white back, peony, clove, golden, and ointment, immersing it in an extraction solvent, Wherein the lipid-degrading agent is provided as an oil-soluble polymer.
6. The method of claim 5,
Wherein the extraction solvent is provided in an aqueous solution of ethanol in an amount of 5 to 15 times by weight with respect to each of the samples,
Wherein the immersion temperature and immersion time for the extraction solvent are in the range of 40 to 70 ° C and 2 to 7 hours, respectively.
The method according to claim 6,
The product obtained by immersing in the extraction solvent and subjecting it to hot extraction is left for 1 to 3 days, filtered through a filter paper having a permeation size of 100 μm or less, concentrated under reduced pressure at 60 ° C. or lower and freeze- Wherein the composition has lipolytic activity.
The method according to claim 1,
The above-mentioned mixed extract can be obtained by mixing each sample of white back, peony, clove, golden, and ointment, or extracting each of the individual samples of white back, peony, clove, Wherein the cosmetic composition has lipolytic activity.

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