KR102299277B1 - Composition for improving scalp condition comprising flower extract of passiflora edulis - Google Patents

Abstract

The present specification provides a composition comprising a flower extract of Passiflora edulis as an active ingredient. Specifically, such a composition may include a flower extract of Passiflora edulis as an active ingredient. In addition, the composition provided herein exhibits a remarkable effect of treating, preventing or improving hair or scalp diseases by including a flower extract of Passiflora edulis as an active ingredient. In addition, the composition provided herein may exhibit anti-inflammatory effects as well as hair loss prevention and hair promotion effects. Due to this effect, the composition can be used to improve the health of the scalp or hair, and for this purpose, it can be widely used in pharmaceutical, food, or cosmetic fields.

Description

Composition for improving scalp condition comprising a flower extract of Pasiflora edulis

The present specification relates to a composition comprising a flower extract of Passiflora edulis.

Hair loss is a problem that occurs either through natural processes or is promoted chemically through the use of certain therapeutic drugs designed to relieve symptoms such as cancer. This hair loss is accompanied by a lack of hair regrowth leading to partial or complete baldness.

Recently, as the number of modern people with alopecia due to environmental pollution, stress, diet, fatigue, incorrect eating habits, etc. increases, interest in substances that can improve it is increasing. Accordingly, it was a task in the art to find a substance that suppresses hair loss and promotes hair growth, as well as a substance that can protect the scalp.

US 10-0969269 B1

An object of the present specification is to provide a composition comprising a natural extract that can be used for improving hair or scalp diseases.

In order to achieve the above object, the present specification provides a composition for improving hair or scalp diseases comprising a flower extract of Passiflora edulis as an active ingredient.

The composition disclosed herein exhibits a remarkable effect of treating, preventing or ameliorating a hair or scalp disease, and specifically, the disease may be a stress-related disease. In addition, the composition disclosed herein exhibits an anti-hair loss, hair-promoting effect, and an anti-inflammatory effect. Due to these effects, the composition disclosed herein can be used as a composition for improving the health of the scalp or hair.

1 is a graph showing the efficacy of a composition comprising a flower extract of Passiflora edulis according to an aspect of the present invention for inhibiting an inflammatory response caused by a stress substance P (Substance P).
2 is a graph showing the efficacy of protecting dermal papilla cells from a culture medium treated with a stress substance P of a composition comprising a flower extract of Passiflora edulis according to an aspect of the present invention.
3 is a graph showing the efficacy of protecting hair follicles from a culture solution treated with a stress substance P of a composition comprising a flower extract of Passiflora edulis according to an aspect of the present invention.

In one aspect, the present invention may relate to a composition for treating, preventing, or improving hair or scalp diseases, comprising a flower extract of Passiflora edulis as an active ingredient.

In one aspect of the present invention, the composition may be a pharmaceutical, food, or cosmetic composition.

In one aspect, the present invention is a hair or scalp disease treatment, prevention, or improvement, comprising administering a flower extract of Passiflora edulis to an individual in need of treatment, prevention, or improvement of a hair or scalp disease It may be about the method. Specifically, the administration may be by the administration method or dosage described herein.

In one aspect, the present invention may relate to the use of a flower extract of Passiflora edulis for the treatment, prevention, or improvement of hair or scalp diseases.

In one aspect, the present invention may relate to a flower extract of Passiflora edulis for use in the treatment, prevention, or improvement of hair or scalp diseases.

In one aspect of the present invention, the hair or scalp disease, regardless of the cause, may specifically be a stressful hair or scalp disease. Specifically, in one aspect of the present invention, stressful hair or scalp disease may be caused by substance P (Substance P), which is a neuropeptide secreted from nerve terminals when stressed.

In one aspect of the present invention, the stressful hair or scalp disease is one or more selected from the group consisting of itching, dandruff, seborrheic skin or scalpitis, hair loss, alopecia areata, male pattern hair loss, psoriasis, stress inflammation, and sensitive scalp. , but is not limited thereto, and is a concept encompassing all hair or scalp diseases caused by stress.

In one aspect, the present invention may relate to a composition for preventing hair loss or promoting hair comprising a flower extract of Passiflora edulis as an active ingredient.

In one aspect, the present invention may relate to a method for preventing hair loss or promoting hair comprising administering a flower extract of Passiflora edulis to an individual in need of preventing hair loss or promoting hair.

In one aspect, the present invention may relate to the use of a flower extract of Passiflora edulis for preventing hair loss or promoting wool.

In one aspect, the present invention may relate to a flower extract of Passiflora edulis for use in preventing hair loss or promoting wool.

In one aspect, the present invention may relate to an anti-inflammatory composition comprising a flower extract of Passiflora edulis as an active ingredient. Specifically, in one aspect of the present invention, the anti-inflammatory composition may be an anti-inflammatory composition for stress inflammation.

In one aspect, the present invention may relate to a composition for improving scalp or hair condition or health comprising a flower extract of Passiflora edulis as an active ingredient.

In one aspect, the present invention may relate to a method for improving scalp or hair health, comprising administering a flower extract of Passiflora edulis to an individual in need of improvement in scalp or hair condition or health.

In one aspect, the present invention may relate to the use of a flower extract of Passiflora edulis for improving scalp or hair condition or health.

In one aspect, the present invention may relate to a flower extract of Passiflora edulis for use in improving scalp or hair condition or health.

In one aspect, the present invention may relate to a composition for antistress comprising a flower extract of Passiflora edulis as an active ingredient.

In one aspect, the present invention may relate to an anti-stress method comprising administering a flower extract of Passiflora edulis to an individual in need of anti-stress.

In one aspect, the present invention may relate to the anti-stress use of a flower extract of Passiflora edulis.

In one aspect, the present invention may relate to a flower extract of Passiflora edulis for use in anti-stress.

In one aspect, the present invention may relate to a composition for inhibiting neuropeptide substance P (Substance P) activity comprising a flower extract of Passiflora edulis as an active ingredient.

In one aspect of the present invention, the flower extract of Passiflora edulis may be at a concentration of 0.1 ppm to 1000 ppm based on the total volume of the composition comprising the same, but is not limited thereto. Specifically, in one aspect of the present invention, the concentration of the flower extract of Passiflora edulis included in the composition is 0.01 ppm or more, 0.1 ppm or more, 0.5 ppm or more, 1 ppm or more, based on the total volume of the composition. 5ppm or more, 10ppm or more, 15ppm or more, 17ppm or more, 19ppm or more, 20ppm or more, 21ppm or more, 23ppm or more, 25ppm or more, 27ppm or more, 30ppm or more, 33ppm or more, 35ppm or more, 37ppm or more, 40ppm or more, 43ppm or more, 45ppm or more , 47ppm or more, 49ppm or more, 50ppm or more, 51ppm or more, 53ppm or more, 55ppm or more, 57ppm or more, 59ppm or more, 60ppm or more, 65ppm or more, 70ppm or more, 75ppm or more, 80ppm or more, 90ppm or more, 100ppm or more, 110ppm or more, 120ppm or more or more, 130ppm or more, 150ppm or more, 200ppm or more, 300ppm or more, 500ppm or more, 700ppm or more, or 1000ppm or more, but is not limited thereto, but is not limited thereto, and 2000ppm or less, 1000ppm or less, 700ppm or less, 500ppm or less, 300ppm or less, 200ppm or less, 150ppm or more or less, 130ppm or less, 120ppm or less, 110ppm or less, 100ppm or less, 90ppm or less, 80ppm or less, 75ppm or less, 70ppm or less, 65ppm or less, 60ppm or less, 58ppm or less, 56ppm or less, 54ppm or less, 52ppm or less, 50ppm or less, 48ppm or less, 46ppm or less, 44ppm or less, 42ppm or less, 40ppm or less, 38ppm or less, 35ppm or less, 32ppm or less, 30ppm or less, 28pm or less, 26ppm or less, 24ppm or less, 22ppm or less, 20ppm or less, 18ppm or less, 15ppm or less, 10ppm or less, 5ppm or less , 1 ppm or less, 0.5 ppm or less, or 0.1 ppm or less.

In one aspect of the present invention, the neuropeptide substance P (Substance P) is known to be related to the regulation of mood disorders, anxiety, stress, pain, vomiting, and the like. Therefore, if the factors induced by the substance P or changes resulting therefrom can be controlled, it will be possible to prevent hair or scalp diseases caused by stress. In addition, in one aspect of the present invention, material P may have the following chemical formula as having an amino acid sequence as follows. Amino acid sequence of substance P: ArgProLysProGlnGlnPhePheGlyLeuMet (RPKPQQFFGLM).

[Formula]

In one aspect of the present invention, the flower extract of Passiflora edulis may be one or more extracts selected from the group consisting of water, organic solvents, and mixtures thereof. Specifically, in one aspect of the present invention, the organic solvent _{may be at least one selected from the group consisting of C 1} to C ₆ lower alcohol, butylene glycol, and propylene glycol, and more specifically, the lower alcohol may be ethanol. . More specifically, in one aspect of the present invention, the flower extract of Passiflora edulis may be a butylene glycol extract.

In one aspect of the present invention, "extract" includes all substances obtained by extracting the components of natural products, regardless of the extraction method, extraction solvent, extracted components or the form of the extract, and also obtained by extracting the components of natural products. It is a broad concept that includes all substances that can be obtained by processing or treating a substance by another method after extraction, and specifically, the processing or treatment may be an additional fermentation of an extract or an enzyme treatment. It is a broad concept including a fermented product, a concentrate, and a dried product, and specifically, the extract herein may be a fermented product.

In one aspect of the present invention, " Flower extract of Passiflora edulis " is an extraction method, an extraction solvent, regardless of the extracted component or the form of the extract, Passiflora edulis ( Passiflora edulis ) It includes all substances obtained by extracting components, and includes substances obtained through extraction methods including treatment with heat, acid, base, enzymes, etc. in the process of extracting the components. Flora edulis ( Passiflora edulis ) It is a broad concept that includes all substances that can be obtained by processing or treating the substances obtained by extracting the components of the flower by other methods after extraction. Specifically, the processing or treatment may be to additionally ferment or enzymatically process the flower extract of Passiflora edulis. Accordingly, in one aspect of the present invention, the flower extract of Passiflora edulis may be a fermented product.

In one aspect of the present invention, "Flower of Passiflora edulis " is in the form of an extract, or a flower of raw Passiflora edulis , a pulverized product of the crude drug itself, and a dried product of the crude drug , a dried pulverized product of a crude drug, a fermented product of a flower of Passiflora edulis, but is not limited thereto. In addition, the flowers of Passiflora edulis used in the present specification are not limited in the method of obtaining them, and may be used by cultivation or by purchasing commercially available ones. In one aspect of the present invention, if the flower of Passiflora edulis is not necessarily prepared through drying, it is a raw material in a form suitable for extracting the active ingredient of the flower of Passiflora edulis not limited

In one aspect of the present invention, water includes distilled water or purified water, and the organic solvent is alcohol, acetone, ether, ethyl acetate, diethyl ether, ethyl methyl ketone, such as _{C 1} to C _{5 lower alcohol, diethyl ether, ethyl methyl ketone,} At least one selected from the group consisting of butylene glycol and chloroform, but is not limited thereto.

In one aspect of the invention, the Pacific Flora dyulriseu (Passiflora edulis) flower extract may include a flower dyulriseu -C ₁ ~ C ₆ alcohol extract of (Passiflora edulis) in the Pacific flora, specifically, the alcohol is methanol or ethanol.

In one aspect of the invention, the flower of dyulriseu (Passiflora edulis) flower extract the Pacific Flora of dyulriseu (Passiflora edulis) in the Pacific Flora a production process comprising the step of water, extraction with an organic solvent, or a mixture thereof can be obtained by

Specifically, in one aspect of the present invention, the method comprises the steps of extracting a flower of Passiflora edulis (Passiflora edulis); filtering the extract; bleaching and deodorizing; filtering it again; may include. The solvent used in the extraction step may be butylene glycol, specifically, a mixed solvent of 30% by weight of butylene glycol and 70% by weight of distilled water may be used. The filtration step may use a mesh (mesh), specifically, may be to filter the extract in the order of 5 μm, 1 μm, 0.5 μm.

In one aspect of the present invention, the flower extract of Passiflora edulis may be a crude extract of a solvent selected from the group consisting of water, an organic solvent, and a combination thereof. The organic solvent may be C ₁ -C ₆ alcohol or butylene glycol, and specifically, the C ₁ -C ₆ alcohol may be methanol or ethanol. That according to one aspect of the invention, the extraction was added corresponding to about 5 to 15 times of the flower of Pacific Flora dyulriseu (Passiflora edulis) dyulriseu (Passiflora edulis) flowers to extraction, Pacific Flora with a solvent in a solvent It is preferable, and specifically, it is preferable to extract by adding about 10 times the solvent, but is not limited thereto.

In one aspect of the present invention, extraction may be performed by hot water extraction, ethanol extraction, heat extraction, cold extraction, reflux extraction, reflux cooling extraction, or ultrasonic extraction, and there is no limitation as long as the extraction method is obvious to those skilled in the art. Furnace extraction may be hot water extraction or ethanol extraction.

In one aspect of the present invention, the extraction may be performed at room temperature, but for more efficient extraction, it may be performed under heating conditions, preferably at a temperature of about 40 to 100° C., more preferably at a temperature of about 80° C. can, but is not limited thereto. The extraction time may be performed for about 2 to about 14 hours, specifically 8 hours to 14 hours, more specifically 11 hours to 13 hours, and most specifically 12 hours, but is not limited thereto, and the extraction solvent and extraction time are not limited thereto. It may vary depending on conditions such as temperature. The extraction may be performed at least once or several times in order to obtain a larger amount of the active ingredient, preferably 1 to 5 times, more preferably 3 times consecutive extraction, and the combined extract may be used.

In one aspect of the invention, the flower extract of dyulriseu (Passiflora edulis) in the Pacific flora may include a dyulriseu crude extract of the flower of (Passiflora edulis) in the Pacific Flora such as the organic low polarity of the crude extract It can also be included as a soluble fraction of an organic solvent obtained by further extraction with a solvent. In one aspect of the present invention, the organic solvent may be hexane, methylene chloride, ethyl acetate, n-butanol, or the like, but is not limited thereto. The extract extracted by the above method or a soluble fraction of the extract may be used as it is, but it may be used in the form of an extract by concentration after filtration, or in the form of a dried product after concentration and drying.

In one aspect of the present invention, drying may be evaporation drying, spray drying, freeze drying, and specifically freeze drying may be performed at -50 to -70°C for 3 to 4 days during freeze drying.

In one aspect of the present invention, the formulation of the cosmetic composition is not particularly limited, and may be appropriately selected according to the purpose. For example, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence, pack, soap, cleansing It may be prepared in any one or more formulations selected from the group consisting of foam, cleansing lotion, cleansing cream, body lotion and body cleanser, but is not limited thereto. In addition, in one aspect of the present invention, the cosmetic composition is not particularly limited in formulating, but includes hair tonic, hair nourishing lotion, scalp treatment, hair treatment, hair rinse, hair shampoo, hair lotion, scalp essence, etc. It may be a cosmetic composition having a formulation.

When the formulation of the cosmetic composition according to an aspect of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc as a carrier component Alternatively, zinc oxide or the like may be used.

When the formulation of the cosmetic composition according to an aspect of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additionally propellants such as chlorofluorohydrocarbons, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition according to an aspect of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition according to one aspect of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester as a carrier component; The same suspending agent, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like can be used.

In the case of surfactant-containing cleansing of the cosmetic composition according to an aspect of the present invention, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarco Cinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative or ethoxylated glycerol fatty acid ester and the like can be used.

The cosmetic composition according to one aspect of the present invention may further include functional additives and components included in general cosmetic compositions in addition to the flower extract of Passiflora edulis. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extract.

In one aspect of the present invention, the cosmetic composition may further contain a component included in a general cosmetic composition as needed along with the functional additive. Other ingredients included include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation An accelerator, a cooling agent, a limiting agent, purified water, etc. are mentioned.

Moreover, in one aspect, the present invention relates to an external preparation for skin comprising a flower extract of Passiflora edulis as an active ingredient, and the external preparation for skin is a generic term that can include anything applied outside the skin. , cosmetics of various formulations may be included here.

The pharmaceutical composition according to one aspect of the present invention may be in various oral or parenteral formulations. In the case of formulation, it is prepared using diluents or excipients, such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, soft or hard capsules, etc., and these solid preparations include at least one excipient in one or more compounds, for example, starch, calcium carbonate, sucrose. Or it is prepared by mixing lactose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, internal solutions, emulsions, syrups, etc. In addition to commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, and the like can be used.

In one aspect of the present invention, the pharmaceutical dosage form of the composition may be used in the form of a pharmaceutically acceptable salt thereof, and may be used alone or in combination with other pharmaceutically active compounds as well as in an appropriate group. The salt is not particularly limited as long as it is pharmaceutically acceptable, and for example, hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrofluoric acid, hydrobromic acid, formic acid acetic acid, tartaric acid, lactic acid, citric acid, fumaric acid, maleic acid, succinic acid, methanesulfonic acid , benzenesulfonic acid, toluenesulfonic acid, naphthalenesulfonic acid, and the like can be used.

In one aspect of the present invention, the composition may be administered parenterally or orally as desired, and administered in an amount of 0.1 to 500 mg per kg of body weight, preferably 1 to 100 mg per day, 1 to several times. It can be administered in divided doses. The dosage for a specific patient may vary depending on the patient's weight, age, sex, health status, diet, administration time, administration method, excretion rate, severity of disease, and the like.

The pharmaceutical composition according to one aspect of the present invention is an oral dosage form such as powder, granule, tablet, soft or hard capsule, suspension, emulsion, syrup, aerosol, etc., ointment, external preparation for skin such as cream, It can be formulated and used in any form suitable for pharmaceutical preparations, including suppositories, injections, and sterile injection solutions, and preferably used in the form of injections or external preparations for skin.

The composition according to an aspect of the present invention may be administered to mammals such as rats, mice, livestock, and humans by various routes such as parenteral and oral administration, and all modes of administration can be expected, for example, It can be administered orally, transdermally, rectally or by intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

The composition according to one aspect of the present invention may be administered by various routes that can be easily applied by those skilled in the art. In particular, the pharmaceutical composition according to one aspect of the present invention may be administered as an external preparation for the skin by a route applied to the skin surface.

In one aspect of the present invention, the food composition may be a health functional food composition.

The formulation of the food composition according to one aspect of the present invention is not particularly limited, but may be formulated as, for example, tablets, granules, powders, liquids such as drinks, caramel, gels, bars, and the like. In addition to the active ingredient, the food composition of each dosage form can be appropriately selected by those skilled in the art without difficulty depending on the dosage form or purpose of use in addition to the active ingredient, and a synergistic effect may occur when applied simultaneously with other raw materials.

In the food composition according to one aspect of the present invention, the determination of the dosage of the active ingredient is within the level of those skilled in the art, and the daily dosage thereof is, for example, 0.1 mg/kg/day to 5000 mg/kg/day, more specifically As may be 50 mg/kg/day to 500 mg/kg/day, but is not limited thereto, and may vary depending on various factors such as age, health status, and complications of the subject to be administered.

The food composition according to an aspect of the present invention is, for example, chewing gum, caramel products, candy, ice cream, various foods such as confectionery, soft drinks, mineral water, beverage products such as alcoholic beverages, health including vitamins and minerals It may be functional foods.

In addition to the above, in one aspect of the present invention, the food composition includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavoring agents, coloring agents and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, in one aspect of the present invention, the functional food compositions may include natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of these additives is not so important, but in one aspect of the present invention, it is generally included in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition.

Hereinafter, the configuration and effect of the present invention will be described in more detail with reference to Examples and Test Examples. However, these Examples and Test Examples are provided only for the purpose of illustration to help the understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

[Example 1] Passiflora edulis ( Passiflora edulis ) Preparation of flower extract

Only the flower parts of Passiflora edulis were collected and extracted by immersing 1 kg of flowers in 32 liters of solvent at 25° C. for 4 days. As the solvent, butylene glycol was used based on the total weight of the solvent. A mixed solvent of 30% by weight and 70% by weight of distilled water was used. Thereafter, the extract was filtered using a mesh in the order of 5 μm, 1 μm, and 0.5 μm, and then decolorization and deodorization processes were performed. This was performed under the condition of stirring at 500 rpm for 1 hour using 0.5% Norit, and activated carbon treatment using an industrial stirrer. Then, using a mesh again, it was filtered in the order of 5 μm, 1 μm, and 0.5 μm. Finally, after filtration, 25 Kg of a flower extract of Passiflora edulis was obtained. In the following experiment, an experiment was conducted using a flower extract of Passiflora edulis , which was commercially obtained from Bioland Co., Ltd. as prepared according to the above method.

[Experimental Example 1] Cell culture

Immune cells, mast cell cell line RBL-2H3 (KCLB (Korea Cell Line Bank) 22256) was added to DMEM medium (Dulbecco's Modified Eagle's Medium) with 10% by weight of fetal bovine serum based on the total weight of the medium. , 100mg/ml of penicillin, and 100mg/ml of streptomycin were added and cultured at _{37°C, 5% CO 2 conditions.}

In addition, human hair dermal papilla cells (hDPC) constituting hair follicles and generating hair were directly isolated and obtained from human hair follicle samples supplied from the Department of Dermatology, Dankook University Hospital. These human dermal papilla cells correspond to cells directly separated from the hair follicle sample remaining after hair follicle transplantation using a scalpel and forceps. _{These human dermal papilla cells were cultured at 37° C., 5% CO 2} condition by adding 20 wt% of fetal bovine serum, 100 mg/ml penicillin, and 100 mg/ml streptomycin to DMEM medium.

[Experimental Example 2] In vitro evaluation of stress-induced inflammatory response

In order to evaluate the inhibitory effect of the flower extract of Passiflora edulis on the stress inflammatory response, mast cells were used to induce and inhibit the inflammatory response. Mast cells cultured according to Experimental Example 1 were inoculated into a 96-well culture plate at 5×10 ⁴ per well and adhered for one day. At this time, the culture medium was used by adding 10% by weight of fetal bovine serum, penicillin 100mg/ml, and streptomycin 100mg/ml to DMEM based on the total weight of the medium.

One day later, when stressed to induce a stressful inflammatory response, 10 uM of substance P, a neuropeptide secreted from nerve terminals, was treated, and the medium was DMEM (phenol red free). 100 mg/ml of penicillin and 100 mg/ml of streptomycin were used. In the experimental group to see the inflammatory response inhibitory effect of the flower extract of Passiflora edulis , the flower extract of Example 1 was treated at a concentration of 20 and 50 ppm in addition to the substance P. The concentration of this extract, ppm, is based on volume, and a 1% (10,000ppm) solution of 10ul of passionflower extract diluted in 1ml of distilled water was treated at 20 and 50ppm, and the ppm concentration of the passionflower extract was used in the same way in the experiment below. . As a positive control, ketotifen, a well-known mast cell stabilizer, was used at a concentration of 13 ppm. After treatment for one day for intracellular drug absorption and inflammatory response, mast cell degranulation reaction analysis (β-hexosaminidase assay), which is one of the inflammatory response evaluation methods, was performed.

For mast cell degranulation reaction, cells were further treated with calcium ionophore (A23187 (Sigma-Aldrich) 1uM + PMA 40nM) as a degranulation activator. After 2 hours, 50ul of cell culture and reaction products were removed from the 96-well culture plate in which the cells were reacted, and dispensed into a new 96-well plate. 50ul of a substrate buffer (1mM p-nitrophenyl-N-acetyl-bD-glucosaminide in 0.1M sodium citrate, pH4.5) required for the color reaction of the degranulation product was added thereto, followed by reaction at 37°C for 1 hour. Thereafter, 150 ul of stop buffer (stop buffer, 0.1M Na ₂ CO ₃ , NaHCO ₃ , pH 10) was added to stop the reaction and induce color development.

The degranulation product of mast cells is yellow as a result of the reaction. To measure this, absorbance was measured with a spectrophotometer at a wavelength of 410 nm. After measurement, when the response result value of the stress neuropeptide (Substance P) treatment group was 100%, the degree of decrease compared to the neuropeptide treatment group was expressed as the inhibitory value of the inflammatory response by the flower extract, which is shown in FIG. It was.

Referring to FIG. 1 , it was confirmed that the degranulation reaction of mast cells occurred in the stress-treated group, but the mast cell degranulation reaction was significantly inhibited when the flower extract was treated. The inhibition of mast cell degranulation reaction by the flower extract shows an effect almost similar to that of ketotifen, which is a stabilizer of mast cells, and through this, the flower extract, which is an aspect of the present invention, has a remarkable effect in suppressing the inflammatory response due to stress. It was confirmed that it worked.

[Experimental Example 3] In vitro evaluation of dermal papilla cell protection from stress

In order to evaluate the protective efficacy of dermal papilla cells from stress of a flower extract of Passiflora edulis, the degree of inhibition of proliferation of human dermal papilla cells was tested. Human dermal papilla cells cultured according to Experimental Example 1 were ^{inoculated into a 96-well culture plate at 2x10 3} per well, and then adhered to the bottom of the culture plate for one day. In this case, as the culture medium, 20% by weight of fetal bovine serum, 100 mg/ml penicillin, and 100 mg/ml streptomycin were added to the DMEM medium based on the total weight of the medium.

One day later, cells were treated with the flower extract of Example 1 at a concentration of 50 ppm, and as positive controls, 13 ppm of ketotifen, a mast cell stabilizer, and 50 μM of minoxidil, a hair growth drug, were used. At this time, as the medium, 100 mg/ml of penicillin and 100 mg/ml of streptomycin were added to the DMEM medium. One day after drug treatment, in order to give a stress environment to human dermal papilla cells, the mast cell reaction solution (culture solution after treatment with Substance P, A23187 + PMA to mast cells) (stress culture solution) prepared in Test Example 2 was used based on the total volume of the well was added to a final concentration of 25% by volume and reacted for two days (48 hours).

WST-1 assay was performed to evaluate the proliferative capacity of dermal papilla cells. 10ul of WST-1 solution was added to the 96-well culture plate in which the cells were reacted, followed by reaction at 37°C for 1 hour. Then, the culture medium color changed from red to orange, and absorbance was measured with a wavelength of 490 nm in a spectrophotometer to measure the change in proliferation of dermal papilla cells. After measurement, the result of the mast cell reaction solution treated group (control group) that was not treated with the stress neuropeptide (Substance P) was 100%, which is shown in FIG. 2 .

Referring to FIG. 2, considering that the proliferation degree of hair follicle cells was 82% in the stress culture medium treatment group, dermal papilla cell proliferation was inhibited. On the other hand, when the flower extract according to one aspect of the present invention is treated, it can be confirmed that the proliferation degree of hair follicle cells is increased to 100%, and from this, it inhibits the dermal papilla cell proliferation inhibitory response due to the stress response, so that the cell proliferation is normal confirmed to happen. This flower extract showed a superior effect than ketotifen, which is a stabilizer for mast cells, and it was confirmed that it showed an effect similar to that of minoxidil, a hair growth drug.

[Experimental Example 4] Human hair follicle protection evaluation from stress

Human hair follicle samples were obtained from byproducts left from hair transplant surgery, which were supplied by the Department of Dermatology, Dankook University Hospital . After separating the sample into a single hair follicle, it was cultured in a cell incubator (5% CO ² , 37° C. The culture medium was Williams medium E, 2 mM L-glutamine, 0.1% fungizone, 10 μg/ml streptomycin) (streptomycin) and 100 U/ml penicillin were used, and the medium was replaced with fresh medium every two days.

On the first day of culture, the experimental group was treated with 50 ppm of the flower extract of Example 1, and minoxidil was treated with 20 ppm as a positive control. After 1 day of drug treatment in the experimental group, the mast cell reaction solution prepared in Test Example 2 (culture solution after treatment with Substance P, A23187 + PMA) (stress culture solution) prepared in Test Example 2 to give a stress environment to human hair follicles was added to a final concentration of 25% was added to become Hair growth length was measured on the 3rd and 5th days of drug treatment in the experimental group. The measurement results are shown in FIG. 3 below.

According to FIG. 3 , hair growth was inhibited in the stress culture medium (CM) alone treatment group, and at this time, hair follicles were protected by the flower extract, and it was confirmed that hair growth occurred at a level similar to that of minoxidil, a positive control group. Therefore, it can be confirmed that the flower extract according to one aspect of the present invention has a hair loss prevention effect and a wool effect similar to minoxidil.

Formulation examples of the composition according to an aspect of the present specification will be described below, but other various formulations are also applicable, which is not intended to limit the present specification, but only to be specifically described.

[Formulation Example 1] Soft capsule

Example 1 Passiflora edulis flower extract 8mg, vitamin E 9mg, vitamin C 9mg, palm oil 2mg, hydrogenated vegetable oil 8mg, yellow wax 4mg and lecithin 9mg, mixed according to a conventional method to a soft capsule Prepare the filling solution. Soft capsules are prepared by filling 400 mg per capsule. And, separately from the above, a soft capsule sheet was prepared in a ratio of 66 parts by weight of gelatin, 24 parts by weight of glycerin, and 10 parts by weight of sorbitol solution, and the filling solution was filled to prepare a soft capsule containing 400 mg of the composition according to the present specification.

[Formulation Example 2] Tablet

Example 1 Passiflora edulis flower extract 8 mg, vitamin E 9 mg, vitamin C 9 mg, galactooligosaccharide 200 mg, lactose 60 mg and maltose 140 mg were mixed and granulated using a fluid bed dryer. Add 6 mg of sugar ester. 500 mg of these compositions are compressed in a conventional manner to prepare tablets.

[Formulation Example 3] Drink agent

Example 1 Passiflora edulis flower extract 8mg, vitamin E 9mg, vitamin C 9mg, glucose 10g, citric acid 0.6g, and liquid oligosaccharide 25g was mixed, then 300㎖ purified water was added to each bottle 200㎖ Fill it up to After filling the bottle, it is sterilized at 130° C. for 4 to 5 seconds to prepare a drink.

[Formulation Example 4] Granules

Example 1 Passiflora edulis flower extract 8mg, vitamin E 9mg, vitamin C 9mg, anhydrous crystalline glucose 250mg, and starch 550mg were mixed, and then molded into granules using a fluidized bed granulator Filling to prepare granules.

[Formulation Example 5] Injection

Injections were prepared in a conventional manner according to the composition shown in Table 1 below.

compounding ingredients content Example 1 Passiflora edulis ( Passiflora edulis ) flower extract 10-50 mg Sterile distilled water for injection appropriate amount pH adjuster appropriate amount

[Formulation Example 6] Health functional food

Health functional foods were prepared in a conventional manner according to the composition shown in Table 2 below.

compounding ingredients content Example 1 Passiflora edulis ( Passiflora edulis ) flower extract 20mg vitamin A acetate 70μg vitamin E 1.0mg vitamin B1 0.13mg vitamin B2 0.15mg vitamin B6 0.5mg vitamin B12 0.2μg vitamin C 10mg biotin 10 μg Nicotinamide 1.7mg folic acid 50 μg Calcium Pantothenate 0.5mg ferrous sulfate 1.75mg zinc oxide 0.82mg magnesium carbonate 25.3mg monobasic potassium phosphate 15mg Dibasic Calcium Phosphate 55mg Potassium Citrate 90mg calcium carbonate 100mg magnesium chloride 24.8mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for health functional food, but the composition is mixed as a preferred example, but the mixing ratio may be arbitrarily modified.

[Formulation Example 7] Health drink

Health drinks were prepared in a conventional manner according to the composition shown in Table 3 below.

compounding ingredients content Example 1 Passiflora edulis ( Passiflora edulis ) flower extract 1000mg citric acid 1000mg oligosaccharide 100 g Taurine 1 g Purified water remaining amount

After mixing the above ingredients according to a conventional health drink manufacturing method, stirring and heating at 85° C. for about 1 hour, the resulting solution is filtered and sterilized.

[Formulation Example 8] Softening lotion (skin lotion)

A softening lotion was prepared in a conventional manner according to the composition shown in Table 4 below.

compounding ingredients content (% by weight) Example 1 Passiflora edulis ( Passiflora edulis ) flower extract 0.2 glycerin 3.0 butylene glycol 2.0 propylene glycol 2.0 Carboxyvinyl Polymer 0.1 PEG-12 Nonylphenyl Ether 0.2 Polysorbate 80 0.4 ethanol 10.0 triethanolamine 0.1 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 9] Nutrient lotion (milk lotion)

Nutrient lotion was prepared in a conventional manner according to the composition shown in Table 5 below.

compounding ingredients content (% by weight) Example 1 Passiflora edulis ( Passiflora edulis ) flower extract 1.0 glycerin 3.0 butylene glycol 3.0 propylene glycol 3.0 Carboxyvinyl Polymer 0.1 beeswax 4.0 Polysorbate 60 1.5 Caprylic/Capric Triglycerides 5.0 squalane 5.0 Sorbitasequioleate 1.5 liquid paraffin 0.5 cetearyl alcohol 1.0 triethanolamine 0.2 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 10] Nourishing Cream

Nutrient creams were prepared in a conventional manner according to the composition shown in Table 6 below.

compounding ingredients content (wt%) Example 1 Passiflora edulis ( Passiflora edulis ) flower extract 2.0 glycerin 3.0 butylene glycol 3.0 liquid paraffin 7.0 beta glucan 7.0 Carbomer 0.1 Caprylic/Capric Triglycerides 3.0 squalane 5.0 cetearyl glucoside 1.5 Sorbitan Stearate 0.4 Polysorbate 60 1.2 triethanolamine 0.1 Preservatives, Colors, Flavors appropriate amount Purified water remaining amount

[Formulation Example 11] Preparation of hair lotion

A hair lotion was prepared according to a conventional method with the composition shown in Table 7 below.

ingredient content (wt%) Example 1 Passiflora edulis ( Passiflora edulis ) flower extract 2.0 glycerin 3.0 Butylene glycol 3.0 liquid paraffin 7.0 beta glucan 7.0 Carbomer 0.1 Caprylic Capric Triglyceride 3.0 squalene 5.0 Cetearyl Grucoside 1.5 Sorbitan Stearate 0.4 polysorbate 1.2 antiseptic appropriate amount incense appropriate amount pigment appropriate amount triethanolamine 0.1 Purified water remaining amount

[Formulation Example 12] Preparation of hair shampoo

A hair shampoo was prepared according to a conventional method with the composition shown in Table 8 below.

ingredient content (wt%) Example 1 Passiflora edulis ( Passiflora edulis ) flower extract 2.0 Sodium Lauryl Sulfate Solution 40 Cocamidopropyl Betaine 5 Cocamide M.A. 1.0 Polyquaternium 10 0.3 methylparaben 0.1 Spices 1.0 Purified water remaining amount

As the specific parts of the present specification have been described in detail above, for those of ordinary skill in the art, these specific techniques are only preferred embodiments, and it is clear that the scope of the present specification is not limited thereto. Accordingly, the substantial scope of the present specification will be defined by the appended claims and their equivalents.

Claims (12)

A food composition comprising:
The composition comprises a flower extract of Passiflora edulis as an active ingredient,
The composition is a composition for preventing or improving stress-induced hair or scalp disease caused by neuropeptide substance P (Substance P),
The hair or scalp disease is at least one selected from the group consisting of itching, dandruff, seborrheic skin, psoriasis and sensitive scalp, the composition. A cosmetic composition comprising:
The composition comprises a flower extract of Passiflora edulis as an active ingredient,
The composition is a composition for preventing or improving stress-induced hair or scalp disease caused by neuropeptide substance P (Substance P),
The hair or scalp disease is at least one selected from the group consisting of itching, dandruff, seborrheic skin, psoriasis and sensitive scalp, the composition. A pharmaceutical composition comprising:
The composition comprises a flower extract of Passiflora edulis as an active ingredient,
The composition is a composition for the treatment, prevention or improvement of stress hair or scalp diseases caused by neuropeptide substance P (Substance P),
The hair or scalp disease is at least one selected from the group consisting of itching, dandruff, seborrheic skin, psoriasis and sensitive scalp, the composition. 4. The method according to any one of claims 1 to 3,
The Passiflora edulis ( Passiflora edulis ) The flower extract is a composition in a concentration of 0.1ppm to 1000ppm based on the total volume of the composition. 4. The method according to any one of claims 1 to 3,
The Passiflora edulis ( Passiflora edulis ) The flower extract is water, an organic solvent, and a composition in which one or more extracts selected from the group consisting of mixtures thereof. 6. The method of claim 5,
The organic solvent is _{at least one composition selected from the group consisting of C 1} ~ C ₆ lower alcohol, butylene glycol, and propylene glycol. delete delete delete delete delete delete

Images