JP2003048812A - Elastase inhibitor and antiaging cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide both an elastase inhibitor having an excellent elastase inhibitory activity and carrying out prevention, prophylaxis and amelioration of the aging of the skin and an antiaging cosmetic using the elastase inhibitor. SOLUTION: The elastase inhibitor comprising an extract from a plant selected from Filipendula ulmaria Maxim, Passiflora incarnata Linne, Verbascum densiflorum Bertol., Solidgo virgaurea L., Viscum album L. and a leaf of Simmondsia chinensis Schneider as an active ingredient and the antiaging cosmetic using the same are provided.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an elastase inhibitor having an excellent elastase inhibitory effect effective for preventing skin aging and an anti-aging cosmetic composition using the elastase inhibitor.

[0002]

2. Description of the Related Art In aging skin, collagen fibers, elastin fibers, and acidic mucopolysaccharides, which are dermal matrix components, are qualitatively and quantitatively changed as the activity of fibroblasts decreases. The collagen fibers become stiff due to the formation of abnormal aging crosslinks, and the original elasticity is lost. The elastin fibers are denatured and destroyed, and elastin, which has a different amino acid composition, is compensatoryly produced to cause functional impairment.
As a result, the skin loses flexibility and wrinkles and sagging occur. In these physiologically aged skin, a decrease in proliferative ability and a physiological function are observed, whereas in a photoaged skin, fibroblasts are increased and proliferated, and collagen production ability is also enhanced. One of the features that best describes this striking contrast is skin thickness, which is strongly thickened in photoaged facial skin,
Physiologically aged skin on the inside of the forearm is usually getting thinner. When considering the aging of human skin, it is necessary to use a protection method corresponding to these contradictory phenomena. Degradation and destruction of elastin, which is one of the causes of aging, is caused by overexpression of elastase, an elastin-degrading enzyme,
Therefore, suppressing the activity of elastase imparts elasticity and firmness to the skin and is important for preventing skin aging. To date, several elastase inhibitors have been developed for the purpose of preventing these skin aging (Japanese Patent Laid-Open No. 9-8713).
6, JP-A-9-87137, JP-A-9-95420, JP-A-95436, JP-A-10-17460, and JP-A-10-104460.
-29925, JP-A-10-29926, and JP-A-10-
36281, JP-A-10-45555, and JP-A-10-1
82414, JP-A-11-92354, JP-A-11-1
47832, JP-A-11-147834, JP-A-11-
171758, JP-A-11-199504, JP-A-11-19994
-246337, JP-A-11-246338, JP-A-1
1-246385, JP-A-11-246386, JP-A-11-246387, JP-A-11-246388, JP-A-11-263720, JP-A-11-315008, JP-A-11-335229, JP-A-11-335230,
JP-A-2000-53578, JP-A-2000-7264
9, JP-A-2000-119189, JP-A-2000-21
2058, JP 2000-247830, and JP 2000
-256176, JP 2000-319189 A, JP 2
001-39822, JP 2001-58920).

[0003]

However, the elastase inhibitors to date have not been sufficiently effective, and effective results have not been obtained when they are incorporated into products.

[0004]

Means for Solving the Problems The present invention provides an elastase inhibitor containing as an active ingredient one or more solvent extracts of the group consisting of Astragalus sinensis, passion flower, velvet squid, goldenrod, mistletoe and jojoba leaves, and these. The present invention provides an anti-aging cosmetic composition containing:

[0005] The citrus cypress that is used in the present invention is a rose family (Rosaceae)
ipendula ulmaria Maxim).

The passionflower used in the present invention means Passiflora incarna (Passifloraceae).
It is the above ground part of taLinne).

[0007] The scorpion squid used in the present invention means Scrophulariaceae.
e) Verbascum den
siflorum Bertol. ) Is above ground.

The goldenrod used in the present invention refers to the Asteraceae (Compositae) goldenrod (Solidago virgaurea L.), and other Solidago gigantean Ai.
t. Solidago Canada L.
Above ground.

The mistletoe used in the present invention includes mistletoe (Viscum album L.) and mistletoe (Viscum album L. var.col.
oratum).

The jojoba leaf used in the present invention means jojoba (Simmondsia chinensis Sch).
neider) leaves.

The method for preparing the extract used in the present invention is not particularly limited, and examples thereof include a method in which various solvents are used and extraction is performed from low temperature to under heating.

Specific examples of the extraction solvent include water, lower monohydric alcohols such as methanol and ethanol, glycerin,
Propylene glycol, dipropylene glycol, 1,
A liquid polyhydric alcohol such as 3-butylene glycol and a lower alkyl ester such as ethyl acetate are exemplified, and one or a mixed solvent of two or more of these may be used.

The extract used in the present invention may be used as it is, or may be filtered and concentrated if necessary. In addition, the extract is subjected to column chromatography, countercurrent distribution, etc.
It can also be used after fractionation and purification.

Furthermore, after drying the above under reduced pressure or freeze-drying, it can be prepared into powder or paste, and can be appropriately formulated to be used.

(Preparation Example 1 of Extract) Preparation of extract of Astragalus sinensis A 100 vol.
Add 000 g, extract at 50 ° C. for 8 hours, cool, and filter to produce an extract of Astragalus membranaceus. The evaporation residue of the product was 1.83%.

(Preparation Example 2 of Extract) Preparation of Extract of Passion Flower 2000 g of 20 vol% ethanol solution is added to 100 g of passion flower and extracted at 50 ° C. for 8 hours. This is filtered and the filtrate is concentrated to dryness under reduced pressure. 3 dry matter
After dissolving in 1000 g of 0% by weight (hereinafter, simply referred to as “%”) 1,3-butylene glycol, filtration is performed to produce a passionflower extract. The evaporation residue of the product is 2.
It was 14%.

(Production Example 3 of Extract) Production of Extract of Scutellaria japonica Extract 100% of Scutellaria striata 20% ethanol solution 2
Add 000 g and extract at 50 ° C. for 8 hours. This is filtered and the filtrate is concentrated to dryness under reduced pressure. 30% dry matter
After dissolving in 1000 g of 1,3-butylene glycol,
Filtrate to produce a Viola versicolor extract. The evaporation residue of the product was 2.56%.

(Extract Production Example 4) Production Method of Goldenrod, Extract 20% 20% ethanol solution in 100g of Goldenrod
Add 00 g and extract at 50 ° C. for 8 hours. This is filtered and the filtrate is concentrated to dryness under reduced pressure. 50% dry matter
After dissolving in 1000 g of 3-butylene glycol, it is filtered to produce a goldenrod extract. The evaporation residue of the product was 2.32%.

(Extract Production Example 5) Production Method of Mistletoe Extract 1000 g of 30% 1,3-butylene glycol is added to 100 g of mistletoe and extraction is performed at room temperature for 5 days. This is filtered to produce a mistletoe extract. The evaporation residue of the product is
It was 1.65%.

(Production Example 6 of extract) Production method of jojoba leaf extract 200 g of jojoba leaf and 200 vol.
0 g was added, and the mixture was stirred and extracted at 60 ° C. for 8 hours, cooled, filtered, and then concentrated, and the synthetic adsorbent Diaion HP-20 was added.
Pass through the column packed with. After washing with 10 vol% ethanol solution, elution with 50 vol% ethanol solution is carried out. The eluate is concentrated under reduced pressure, dried and dissolved in 300 g of 30% ethanol solution to produce a jojoba leaf extract. The evaporation residue of the product was 1.56%.

The extract of the present invention can be used as it is as an elastase inhibitor, or can be compounded as an anti-aging cosmetic composition in external preparations for skin and bath agents, but the compounding amount is not particularly limited. Although it varies depending on the type of product to be blended, properties, quality, and the degree of expected effect, it is preferably 0.0001 to 10.0%, particularly 0.001 to 5.0% in terms of dry solid matter. It is preferable from the aspect.

The elastase inhibitor of the present invention and the anti-aging cosmetic compounded with the ingredient may be used as necessary in the range that does not impair the effects of the present invention, such as components used in medicines, quasi drugs, cosmetics and the like. It can be manufactured by using additives together. Specific examples of these additive components are as follows.

As the surface active agent, a base material for soap, fatty acid soap, higher alkyl sulfate ester, alkyl ether sulfate ester salt, N-acyl sarcosinic acid, higher fatty acid amide sulfonate salt, phosphate ester salt, sulfosuccinate salt, alkylbenzene. Sulfonate, N-acyl glutamate, higher fatty acid ester sulfate ester salt, sulfated oil, POE alkyl ether carboxylate, POE
Alkyl allyl ether carboxylate, α-olefin sulfonate, higher fatty acid ester sulfonate, secondary alcohol sulfate ester salt, higher fatty acid alkylolamide sulfate ester salt, lauroyl monoethanolamide succinate salt, N-palmitoyl aspartic acid Anionic surfactants such as ditriethanolamine and sodium caseinate. Alkyl trimethyl ammonium salt, dialkyl dimethyl ammonium salt, alkyl pyridium salt, alkyl quaternary ammonium salt, alkyl dimethyl benzyl ammonium salt, alkyl isoquinonium salt, dialkyl morphonium salt, POE alkyl amine, alkyl amine salt, polyamine fatty acid derivative , Cationic surfactants such as amyl alcohol fatty acid derivatives, benzalkonium chloride and benzethonium chloride. Amphoteric surfactants such as imidazoline-based surfactants and betaine-based surfactants. Lipophilic nonionic surfactants such as sorbitan fatty acid ester, glycerin fatty acid ester, propylene glycol fatty acid ester, hydrogenated castor oil derivative, glycerin alkyl ether, and polyoxyethylene / methylpolysiloxane copolymer. POE sorbitan fatty acid ester, POE sorbitol fatty acid ester, POE glycerin fatty acid ester, POE fatty acid ester, POE alkyl ether, POE alkyl phenyl ether, POE / POP
Alkyl ether, tetra POE / tetra POP ethylenediamine condensate, POE hydrogenated castor oil derivative, POE
Beeswax lanolin derivative, alkanolamide, PO
Examples include hydrophilic nonionic surfactants such as E propylene glycol fatty acid ester, POE alkyl amine, POE fatty acid amide, and sucrose fatty acid ester.

The oils include avocado oil, olive oil,
Sesame oil, camellia oil, evening primrose oil, turtle oil, macadamian nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, wheat germ oil, southern oil, castor oil, linseed oil, safflower oil, cottonseed oil, Eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, tung oil,
Animal and vegetable oils such as jojoba oil, cacao butter, palm oil, horse oil, palm oil, palm kernel oil, beef tallow, sheep fat, lard, lanolin, spermaceti, beeswax, carnauba wax, mokuro, candelilla wax, squalane, and its hardening. oil. Mineral oil such as liquid paraffin and petrolatum. There are synthetic triglycerins such as glycerin tripalmitate.

Examples of the higher fatty acid include lauric acid,
Examples include myristic acid, palmitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid, behenic acid, 12-hydroxystearic acid, isostearic acid, undecic acid, tallic acid, eicosapentaenoic acid, and docosahexaenoic acid. Examples of higher alcohols include lauryl alcohol, cetyl alcohol, stearyl alcohol, behenyl alcohol, myristyl alcohol,
Oleyl alcohol, cetostearyl alcohol, jojoba alcohol, lanolin alcohol, batyl alcohol, 2-decyltetrathecesinol, cholesterol,
Examples include phytosterol and isostearyl alcohol. Examples of synthetic esters include cetyl octanoate, octyldodecyl myristate, isopropyl myristate, myristyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate,
Decyl orenic acid, dimethyl octanoic acid, cetyl lactate,
Examples include myristyl lactate. Examples of silicones include chain polysiloxanes such as dimethylpolysiloxane and methylphenylpolysiloxane, cyclic polysiloxanes such as decamethylcyclopolysiloxane, and those having a three-dimensional network structure such as silicone resin.

Examples of moisturizers include glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, polyethylene glycol, hexylene glycol, xylitol, sorbitol, maltitol, chondroitin sulfate, hyaluronic acid, mucoitin sulfate, atelocollagen. , Urea, sodium lactate,
In addition to bile salts, dlpyrrolidone carboxylates, soluble collagen, and the like, there are various animal and plant extracts, yeast extracts and the like.

Examples of the ultraviolet absorber include benzoic acid type ultraviolet absorbers such as paraaminobenzoic acid and paraaminobenzoic acid derivatives, anthranilic acid type ultraviolet absorbers such as homomenthyl-N-acetylanthranilate, and salicylic acid type absorbers such as amyl sacylate. UV absorbers, cinnamic acid-based UV absorbers such as octylcinnamate, benzophenone-based UV absorbers such as 2,4-dihydroxybenzophenone, 4-methylbenzylidene camphor, 3-benzylidene camphor, 2-phenyl-5-methylbenzoxa There are sols.

Examples of vitamins include vitamin A such as vitamin oil and retinol, vitamin B2 such as riboflavin, and vitamin B6 such as pyridoxine hydrochloride.
, Vitamin Cs such as L-ascorbic acid, pantothenic acids such as calcium pantothenate, vitamin Ds such as ergocalciphenol, nicotinic acids such as nicotinamide, vitamin Es such as tocophenol acetate, vitamin P, biotin Etc.

Examples of the natural water-soluble polymer include gum arabic, tragacanth gum, galactan, guar gum, carob gum, karaya gum, carrageenan, pectin, agar, quince seed, algae colloid, starch,
Examples include xanthan gum, dextran, succinoglucan, pullulan, collagen, casein, hyaluronic acid, albumin and gelatin. Examples of the semi-synthetic water-soluble polymer include cellulosic polymers such as methylcellulose, nitrocellulose and sodium carboxymethylcellulose, starch-based polymers such as carboxymethylstarch, and alginic acid-based polymers such as sodium alginate. Examples of the synthetic water-soluble polymer include vinyl polymers such as polyvinyl alcohol and carboxyvinyl polymer, polyoxyethylene polymers such as polyethylene glycol 2000, and copolymer polymers such as polyoxyethylene polyoxypropylene copolymer. System, acrylic polymer such as polyacrylamide, polyethyleneimine, cationic polymer and the like.

Examples of the powder component include talc, kaolin, mica, sericite, magnesium carbonate, calcium carbonate, silicate, silica, barium sulfate, burnt gypsum, fluoroapatite, inorganic powder such as ceramic powder, nylon powder, and the like. Examples include organic powders such as polyethylene powder, polystyrene powder, and cellulose powder. As the coloring agent, inorganic pigments such as titanium dioxide, iron oxide, carbon black and cobalt violet, organic pigments such as red 201, red 3, yellow 205 and yellow 4, chlorophyll, riboflavin, β-carotene, etc. Natural pigments, plant extract pigments such as safflower and turmeric. Preservatives include benzoate, salicylate, sorbate, dehydroacetate, paraoxybenzoate, benzalkonium chloride, hinokitiol, resorcin, ethanol and the like. Examples of antioxidants include tocophenol, ascorbic acid, butylhydroxyanisole, dibutylhydroxytoluene, and gallic acid ester. Examples of the chelating agent include sodium ethylenediaminetetraacetate, sodium polyphosphate, citric acid and the like.

Further, a plant extract having physiological activity such as antibacterial activity, cell activation, moisturizing, sebum secretion control, antiphlogistic, astringent, antioxidation, whitening, suppression of active oxygen, antiallergy, etc., and their extracted fractions and purified products Can also be used together. Also,
In addition to the above, fragrance, alcohol, water and the like can be appropriately added.

The elastase inhibitor of the present invention and the anti-aging cosmetics containing the same are not limited to general skin cosmetics, but include pharmaceuticals, quasi drugs, cosmeceuticals and the like. is there. The dosage form of the skin external preparation composition of the present invention may be any of a solubilizing system, an emulsifying system, a powder dispersion system, and the like, and is also used for makeup such as lotions, emulsions, creams, basic cosmetics such as packs and foundations. Cosmetics, shampoo, conditioner, soap, toiletries such as body shampoo, and bath agents can be used.

Examples will be described below, but the present invention is not limited to these examples.

Test Example 1 Evaluation of Elastase Inhibitory Activity The inventors of the present invention examined the pancreas-derived elastase (Type I manufactured by Sigma) and Succinyl-L as a synthetic substrate.
-Alanyl-L-alanyl-L-alanin
ep-nitroanilide (manufactured by Sigma)
Was evaluated. A test sample was prepared by adding 50 vol% ethanol solution to 50 g of each plant dried product, extracting at 50 ° C. for 8 hours, filtering and concentrating under reduced pressure, and lyophilized powder was dissolved in purified water to determine the solid concentration of the extract. It was set to 0.5 mg / mL. In addition, Extract Production Example 6 was dissolved in purified water and used as an extract solid content concentration of 0.5 mg / mL.

(Test method) The synthetic substrate is dissolved in DMSO to 15 mM, and 1 mL aliquots are dispensed into microtubes and stored frozen. Before use, dilute 20-fold with 0.1 M HEPES buffer (pH 7.5, 0.5 M NaCl) to 0.75 mM. Elastase is 50 mM
Adjust to 0.07 unit / mL with Tris-HCl buffer (pH 8.5, 1M NaCl). 100 μL of synthetic substrate, 50 μL of elastase, and 50 μL of test sample were added to a 96-well plate, and incubated at 37 ° C. for 2 hours. Then, the absorbance was measured at 405 nm with a plate reader.

From the measurement results, the elastase inhibition rate was calculated by the following formula. Elastase inhibition rate (%) = [1- (AB) / (C-
D)] × 100 A: Absorbance when sample solution is added, elastase is added B: Absorbance when sample solution is added, without elastase C: Absorbance when sample is not added, elastase is added D: When sample is not added, and elastase is not added Absorbance However, when no addition was made, purified water and buffer were used instead. Each inhibition rate is shown in Table 1.

[0037]

[Table 1]

Test Example 2 Skin Anti-aging Test In order to examine the anti-aging effect of skin, the following Examples 1 to 6 were used.
Using the cosmetics having the composition shown in Comparative Example 1, an evaluation test was performed for the effect of improving wrinkles and the effect of improving skin swelling and sagging by the following method.

Sixty randomly selected healthy women aged 40 to 50 were used as test subjects, and 10 of each of the cosmetics of Examples and Comparative Examples were used on the facial skin for 2 days every day, and then the effect of improving wrinkles was obtained. The effect of improving skin swelling and sagging was examined.

Example 1 Cream The following components (1) to (10), and separately the following components (11) to
(16) is dissolved by heating at 75 ° C. to prepare solutions A and B, respectively. Solution B is added to solution A to emulsify, stirring at 50 ° C
The mixture was cooled to 40 ° C. and ingredient (17) was added to prepare a cream. (Component) (% by weight) (1) Jojoba oil 3.0% (2) Squalane 2.0% (3) Methylpolysiloxane 0.5% (4) Stearyl alcohol 0.5% (5) Cetyl alcohol 0. 5% (6) Glyceryl tri (capryl / caprate) 12.5% (7) Glyceryl monostearate 5.0% (8) Diglyceryl monostearate 1.5% (9) Decaglyceryl monostearate 3. 0% (10) Propyl paraoxybenzoate 0.1% (11) Xanthan gum 0.1% (12) Spirea extract (Production Example 1) 3.0% (13) Glycerin 1.0% (14) 1,3 -Butylene glycol 5.0% (15) Methyl paraoxybenzoate 0.2% (16) Purified water 62.0% (17) Perfume 0.1%

(Examples 2 to 6) The passionflower extract of Example 1 was replaced with the passionflower extract (Production Example 2).
Was used in Example 2, the same was used as the velvet squid extract (Production Example 3) in Example 3, and the same was used as the goldenrod extract (Production Example 4) in Example 4 and similarly the mistletoe extract ( Production Example 5) is the same as Example 5,
Similarly, the extract of jojoba leaf (Production Example 6) is referred to as Example 6.

(Comparative Example 1) In Cream Example 1,
A cream was prepared in the same manner as in Example 1 except that 3.0% of the Astragalus sinensis extract was replaced with 3.0% of purified water.

"Improvement Effect on Wrinkles" The state of wrinkles on the outer corners of the eyes was visually evaluated. (Judgment Criteria) Effective: Wrinkles were less noticeable Somewhat effective: Wrinkles were less noticeable than before No effect: No change "Improvement effect on skin swelling and sagging" Skin swelling and sagging were visually evaluated. (Judgment Criteria) Effective: There is more swelling on the skin than before use, and there is no slackness Somewhat effective: There is still some slackness on the skin compared to before use, there is no sagging effect No change: No change

[0044]

[Table 2]

As is clear from Table 2, when the creams of Examples 1 to 6 were used, the wrinkles around the corners of the eyes, the swelling of the skin, and the sagging were improved as compared with the case where the cream of Comparative Example 1 was used. It was recognized that it was done. Thus, it was confirmed that the cosmetics containing the extract of Astragalus sinensis L., the extract of passionflower, the extract of Astragalus edulis, the extract of Astragalus sinensis, the extract of mistletoe, and the extract of jojoba leaf have an anti-aging effect.

The formulation examples of the present invention are shown below.

(Example 7) Toner lotion The following components (5) to (8) were mixed and dissolved to prepare a liquid A. Separately, the following components (1) to (4) and (9) were mixed and dissolved to prepare liquid B. As a liquid, the liquids A and B were evenly mixed to prepare a lotion. (Component) (wt%) (1) quince seed extract 8.0% (2) glycerin 3.0% (3) 1,3-butylene glycol 5.0% (4) Spirea extract (Production Example 1) 2 0.0% (5) Polyoxyethylene sorbitan lauric acid ester 1.2% (6) Ethyl alcohol 5.0% (7) Methyl paraoxybenzoate 0.2% (8) Perfume 0.1% (9) Purified water 75.5%

(Example 8) Emulsion The following components (1) to (10) and separately (11) to (14) and (16) were heated and dissolved at 75 ° C to prepare solutions A and B, respectively. Add Liquid B to emulsify and stir 5
It cooled to 0 degreeC and added the component (15), and prepared the emulsion. (Component) (% by weight) (1) Jojoba oil 1.0% (2) Squalane 2.0% (3) Behenyl alcohol 1.0% (4) Tri (capryl-capric acid) glyceryl 2.0% (5) Tetraglycerin condensed silinoleic acid 0.1% (6) Propylene glycol monooleate 0.5% (7) Glycerin monostearate 1.0% (8) Hexaglyceryl monomyristate 1.0% (9) Deca monomyristate Glyceryl 0.5% (10) Propyl paraoxybenzoate 0.1% (11) Quinceseed extract 5.0% (12) Passionflower extract (Production Example 2) 2.0% (13) Jojoba leaf extract ( Production Example 6) 2.0% (14) 1,3-butylene glycol 3.0% (15) Perfume 0.1% (16) Purified water 78.7%

(Example 9) Soap The following ingredients were mixed and produced by a standard method for producing soap.     (Component) (% by weight)   (1) Soap substrate 53.2%   (2) Scroll 19.4%   (3) Jojoba oil 0.25%   (4) Mistletoe extract (Production Example 5) 2.5%   (5) Jojoba leaf extract (Production Example 6) 2.5%   (6) Concentrated glycerin 6.5%   (7) Hydroxyethanediphosphonic acid 0.15%   (8) Constant water 15.5%

(Example 10) Cleansing gel The following components (1) to (3) and separately (4) to (6) and (8) were dissolved by heating at 70 ° C to prepare liquid A and liquid B, respectively. Solution B is added to and stirred until uniform. It cooled to 50 degreeC, stirring, and the ingredient (7) was added and the cleansing gel was manufactured. (Components) (% by weight) (1) Hexaglyceryl monomyristate 20.0% (2) Liquid paraffin 58.8% (3) Paraoxybenzoic acid ester 0.3% (4) Velvet mullein extract (Production Example 3) 0.5% (5) Goldenrod extract (Production Example 4) 0.5% (6) Concentrated glycerin 5.9% (7) Sorbitol 5.0% (8) Perfume 0.1% (9) Purified water 8 .9%

Example 11 Packing Agent A phase, B phase, and C phase are uniformly dissolved, and phase B is added to phase A to solubilize it, and then phase C is added and dissolved uniformly,
To make. (Component) (% by weight) (Phase A) Dipropylene glycol 5.0% Polyoxyethylene hydrogenated castor oil 5.0% (Phase B) Olive oil 5.0% Tocophenol acetate 0.2% Paraoxybenzoic acid ester 2% (Phase C) Sodium bisulfite 0.03% Polyvinyl alcohol 13.0% Bild mud squid extract (Production Example 3) 1.0% Passionflower extract (Production Example 2) 1.0% Ethanol 7.0% Purified water 62.77%

(Embodiment 12) Emulsion type foundation A is a powder part obtained by thoroughly mixing and pulverizing the following components (1) to (6), (7) and (8) are solutions B, (9) to (12) and Let (14) be liquid C. After the solution C is heated and stirred, the solution A is added and subjected to a homomixer treatment, and then the solution B mixed by heating is added and subjected to a homomixer treatment. It is cooled to 50 ° C. with stirring, (13) is added, and further cooled to room temperature to manufacture. (Components) (% by weight) (1) Titanium dioxide 10.3% (2) Sericite 5.4% (3) Kaolin 3.0% (4) Yellow iron oxide 0.7% (5) Red iron oxide 0.4 % (6) Black iron oxide 0.2% (7) Decamethylcyclopentasiloxane 11.5% (8) Liquid paraffin 8.5% (9) Sorbitan sesquioleate 3.0% (10) Jojoba leaf extract (Production Example 6) 1.5% (11) 1,3-butylene glycol 5.0% (12) Paraoxybenzoic acid ester 0.2% (13) Perfume 0.2% (14) Purified water 50.1%

(Example 13) Solid foundation The following components (1) to (7) were uniformly mixed with a blender,
(8) to (14) are added to this and kneaded well to produce. (Component) (% by weight) (1) Talc 42.4% (2) Kaolin 15.5% (3) Sericite 10.0% (4) Zinc white 7.0% (5) Titanium dioxide 3.8% (6) Yellow iron oxide 2.9% (7) Black iron oxide 0.2% (8) Squalane 8.0% (9) Isostearic acid 4.0% (10) Polyoxyethylene sorbitan monooleate 3.0 % (11) Isocetyl octoate 2.0% (12) Bilus mud squid extract (Production Example 3) 1.0% (13) Paraoxybenzoic acid ester 0.1% (14) Perfume 0.1%

The cosmetics of Examples 7 to 13 were all excellent in the anti-aging effect on the skin.

[0055]

Industrial Applicability As described above, the elastase inhibitor and the anti-aging cosmetic composition of the present invention have excellent elastase inhibitory activity, prevent and improve skin aging, and have elasticity and wrinkle. You can maintain a youthful skin condition without sagging.

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Claims (2)

[Claims] 1. An elastase inhibitor comprising, as an active ingredient, one or more solvent extracts of the group consisting of Astragalus sinensis, passionflower, velvet squid, goldenrod, mistletoe and jojoba leaf. 2. An anti-aging cosmetic composition containing the elastase inhibitor of claim 1.