JP2003048846A - Collagenase inhibitor and antiaging cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a collagenase inhibitor having excellent activities for inhibiting the collagenase activity, and preventing the prophylaxis and the amelioration of aging of the skin, and further to provide an antiaging cosmetic. SOLUTION: The collagenase inhibitor or the antiaging cosmetic contains a solvent extract of a plant selected from Filipendula multijuga, Viscum album, a leaf of jojoba, a leaf of lotus and red clover.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a collagenase inhibitor having an excellent collagenase inhibitory activity which is effective for preventing skin aging, and an anti-aging cosmetic composition using the collagenase inhibitor.

[0002]

2. Description of the Related Art In aging skin, collagen fibers, elastin fibers, and acidic mucopolysaccharides, which are dermal matrix components, are qualitatively and quantitatively changed as the activity of fibroblasts decreases. The collagen fibers become stiff due to the formation of abnormal aging crosslinks, and the original elasticity is lost. The elastin fibers are denatured and destroyed, and elastin, which has a different amino acid composition, is compensatoryly produced to cause functional impairment.
As a result, the skin loses flexibility and wrinkles and sagging occur. In these physiologically aged skin, a decrease in proliferative ability and a physiological function are observed, whereas in a photoaged skin, fibroblasts are increased and proliferated, and collagen production ability is also enhanced. One of the features that best describes this striking contrast is skin thickness, which is strongly thickened in photoaged facial skin,
Physiologically aged skin on the inside of the forearm is usually getting thinner. When considering the aging of human skin, it is necessary to use a protection method corresponding to these contradictory phenomena. In recent years, various studies have been conducted to purify various matrix metalloproteases (MMPs) of the matrixin family, which are considered to play important roles in the organization of tissues and maintenance of homeostasis. Collagenase among these matrix metalloproteases, namely MMP-1, MMP-8, MMP-1
3, MMP-18 has been confirmed so far, but especially M
MP-1 decomposes collagen I and III, which are the main constituents of the skin dermal matrix, and is deeply involved in skin aging. Degeneration and reduction of collagen occur due to overexpression of collagenase, which is a collagen-degrading enzyme,
Therefore, suppressing the activity of collagenase imparts elasticity and firmness to the skin and is important for preventing skin aging. Until now, several collagenase inhibitors and MMP inhibitors have been developed for the purpose of preventing these skin aging (JP-A-9-40552, JP-A-10-194982, JP-A-11-71294, JP-A-11-79970). , JP-A-11
-147833, JP-A-11-315008, JP-A-2
000-154131, Japanese Patent Laid-Open No. 2000-15963.
1, JP-A-2000-191512, JP-A-2000-
212058, JP-A 2000-256176, JP-A 11-79971, JP-A 2000-191487, JP-A 2000-256122, and JP-A 2000-319.
155, JP 2001-139466 A, JP 200
1-192316, Japanese Patent Laid-Open No. 2001-192317).

[0003]

However, the above-mentioned collagenase inhibitors are not always sufficiently effective, and effective results have not been obtained when they are incorporated into products.

[0004]

Means for Solving the Problems The present invention provides a collagenase inhibitor containing one or more solvent extracts of the group consisting of Astragalus sinensis, mistletoe, jojoba leaf, lotus leaf and red clover as an active ingredient, and a combination thereof. The anti-aging cosmetics are provided.

[0005] The citrus cypress that is used in the present invention is a rose family (Rosaceae)
ipendula ulmaria Maxim).

The mistletoe used in the present invention means mistletoe (Viscum album L.) and mistletoe (Viscum album L. var.col.
oratum).

The jojoba leaf used in the present invention means jojoba (Simmondsia chinensis Sch).
neider) leaves.

The lotus leaf used in the present invention means a lotus (Nymphaeaceae) lotus (Nelulnbi).
um nelumbo Druce).

The red clover used in the present invention is a red clover of the legume, Trifolium prat.
ense L. Flowers.

The method for preparing the extract used in the present invention is not particularly limited, and examples thereof include a method in which various solvents are used and extraction is performed from low temperature to under heating.

Specific examples of the extraction solvent include water, lower monohydric alcohols such as methanol and ethanol, glycerin,
Propylene glycol, dipropylene glycol, 1,
A liquid polyhydric alcohol such as 3-butylene glycol and a lower alkyl ester such as ethyl acetate are exemplified, and one or a mixed solvent of two or more of these may be used.

The extract used in the present invention may be used as it is, or may be filtered and concentrated if necessary. In addition, the extract is subjected to column chromatography, countercurrent distribution, etc.
It can also be used after fractionation and purification.

Further, it is possible to use the above-mentioned materials after drying under reduced pressure or freeze-drying, and then preparing into powder or paste and appropriately formulating them for use.

(Preparation Example 1 of Extract) Preparation of extract of Astragalus sinensis Extract 50 g of 50 vol% ethanol solution to 100 g of Astragalus sinensis
Add 000 g, extract at 50 ° C. for 8 hours, cool, and filter to produce an extract of Astragalus membranaceus. The evaporation residue of the product was 1.83%.

(Extract Production Example 2) Production Method of Mistletoe Extract 100 g of mistletoe and 2000 g of 70 vol% ethanol
And extract at room temperature for 5 days. It is filtered and concentrated to dryness. 50% by weight (hereinafter referred to simply as "%") 1,3
-Dissolve in 1000 g of butylene glycol solution with heating to produce a mistletoe extract. The product evaporation residue is 1.15
%Met.

(Extract Production Example 3) Production Method of Jojoba Leaf Extract 200 g of jojoba leaf and 50 vol% ethanol solution 200
0 g was added, and the mixture was stirred and extracted at 60 ° C. for 8 hours, cooled, filtered, and then concentrated, and the synthetic adsorbent Diaion HP-20 was added.
Pass through the column packed with. After washing with water, elute with 50 vol% ethanol solution, dry the eluate under reduced pressure to 30%
It is dissolved in 300 g of an ethanol solution to produce a jojoba leaf extract. The evaporation residue of the product was 2.38%.

(Extract Production Example 4) Method for producing lotus leaf extract 2000 g of 30 vol% ethanol solution per 100 g of lotus leaf
And extracted at 50 ° C. for 8 hours. This is filtered and the filtrate is concentrated to dryness under reduced pressure. The dried solid is dissolved in 1000 g of 50% 1,3-butylene glycol and then filtered to prepare a lotus leaf extract. The evaporation residue of the product was 2.32%.

(Extract Production Example 5) Red clover extract To 200 g of red clover, 2000 g of 50 vol% ethanol solution was added, and the mixture was extracted at 50 ° C. for 8 hours. After cooling, filtration, and concentration, synthetic adsorbent Diaion HP-2
Pass through a column filled with 0. After washing with water, 50 vol%
Elute with an ethanol solution, dry the eluate under reduced pressure and dry it to 50
% 1,3-butylene glycol solution dissolved in 300 g,
Make jojoba leaf extract. The evaporation residue of the product is 2.1
It was 7%.

The extract of the present invention can be used as it is as a collagenase inhibitor, and can also be added as an anti-aging cosmetic to a skin external preparation and a bath preparation, but the amount thereof is not particularly limited. Although it varies depending on the type of product to be blended, properties, quality, and the degree of expected effect, it is preferably 0.0001 to 10.0%, particularly 0.001 to 5.0% in terms of dry solid matter. It is preferable from the aspect.

The collagenase inhibitor of the present invention and the anti-aging cosmetic compounded with it are, if necessary, used in components, pharmaceuticals, quasi drugs, cosmetics, etc., as long as the effects of the present invention are not impaired. It can be manufactured by using additives together. Specific examples of these additive components are as follows.

As the surfactant, a soap base, fatty acid soap, higher alkyl sulfate ester, alkyl ether sulfate ester salt, N-acyl sarcosinic acid, higher fatty acid amide sulfonate, phosphate ester salt, sulfosuccinate, alkylbenzene. Sulfonate, N-acyl glutamate, higher fatty acid ester sulfate ester salt, sulfated oil, POE alkyl ether carboxylate, POE
Alkyl allyl ether carboxylate, α-olefin sulfonate, higher fatty acid ester sulfonate, secondary alcohol sulfate ester salt, higher fatty acid alkylolamide sulfate ester salt, lauroyl monoethanolamide succinate salt, N-palmitoyl aspartic acid Anionic surfactants such as ditriethanolamine and sodium caseinate. Alkyl trimethyl ammonium salt, dialkyl dimethyl ammonium salt, alkyl pyridium salt, alkyl quaternary ammonium salt, alkyl dimethyl benzyl ammonium salt, alkyl isoquinonium salt, dialkyl morphonium salt, POE alkyl amine, alkyl amine salt, polyamine fatty acid derivative , Cationic surfactants such as amyl alcohol fatty acid derivatives, benzalkonium chloride and benzethonium chloride. Amphoteric surfactants such as imidazoline-based surfactants and betaine-based surfactants. Lipophilic nonionic surfactants such as sorbitan fatty acid ester, glycerin fatty acid ester, propylene glycol fatty acid ester, hydrogenated castor oil derivative, glycerin alkyl ether, and polyoxyethylene / methylpolysiloxane copolymer. POE sorbitan fatty acid ester, POE sorbitol fatty acid ester, POE glycerin fatty acid ester, POE fatty acid ester, POE alkyl ether, POE alkyl phenyl ether, POE / POP
Alkyl ether, tetra POE / tetra POP ethylenediamine condensate, POE hydrogenated castor oil derivative, POE
Beeswax lanolin derivative, alkanolamide, PO
Examples include hydrophilic nonionic surfactants such as E propylene glycol fatty acid ester, POE alkyl amine, POE fatty acid amide, and sucrose fatty acid ester.

The oils include avocado oil, olive oil,
Sesame oil, camellia oil, evening primrose oil, turtle oil, macadamian nut oil, corn oil, mink oil, rapeseed oil, egg yolk oil, persic oil, wheat germ oil, southern oil, castor oil, linseed oil, safflower oil, cottonseed oil, Eno oil, soybean oil, peanut oil, tea seed oil, kaya oil, rice bran oil, tung oil,
Animal and vegetable oils such as jojoba oil, cacao butter, palm oil, horse oil, palm oil, palm kernel oil, beef tallow, sheep fat, lard, lanolin, spermaceti, beeswax, carnauba wax, mokuro, candelilla wax, squalane, and its hardening. oil. Mineral oil such as liquid paraffin and petrolatum. There are synthetic triglycerins such as glycerin tripalmitate.

Examples of the higher fatty acid include lauric acid,
Examples include myristic acid, palmitic acid, oleic acid, linoleic acid, linolenic acid, stearic acid, behenic acid, 12-hydroxystearic acid, isostearic acid, undecic acid, tallic acid, eicosapentaenoic acid, and docosahexaenoic acid. Examples of higher alcohols include lauryl alcohol, cetyl alcohol, stearyl alcohol, behenyl alcohol, myristyl alcohol,
Oleyl alcohol, cetostearyl alcohol, jojoba alcohol, lanolin alcohol, batyl alcohol, 2-decyltetrathecesinol, cholesterol,
Examples include phytosterol and isostearyl alcohol. Examples of synthetic esters include cetyl octanoate, octyldodecyl myristate, isopropyl myristate, myristyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate,
Decyl orenic acid, dimethyl octanoic acid, cetyl lactate,
Examples include myristyl lactate. Examples of silicones include chain polysiloxanes such as dimethylpolysiloxane and methylphenylpolysiloxane, cyclic polysiloxanes such as decamethylcyclopolysiloxane, and those having a three-dimensional network structure such as silicone resin.

Examples of moisturizers include glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, polyethylene glycol, hexylene glycol, xylitol, sorbitol, maltitol, chondroitin sulfate, hyaluronic acid, mucoitin sulfate, atelocollagen. , Urea, sodium lactate,
In addition to bile salts, dlpyrrolidone carboxylates, soluble collagen, and the like, there are various animal and plant extracts, yeast extracts and the like.

Examples of the ultraviolet absorber include benzoic acid type ultraviolet absorbers such as paraaminobenzoic acid and paraaminobenzoic acid derivatives, anthranilic acid type ultraviolet absorbers such as homomenthyl-N-acetylanthranilate, and salicylic acid type absorbers such as amyl sacylate. UV absorbers, cinnamic acid-based UV absorbers such as octylcinnamate, benzophenone-based UV absorbers such as 2,4-dihydroxybenzophenone, 4-methylbenzylidene camphor, 3-benzylidene camphor, 2-phenyl-5-methylbenzoxa There are sols.

Examples of vitamins include vitamin A such as vitamin oil and retinol, vitamin B2 such as riboflavin, and vitamin B6 such as pyridoxine hydrochloride.
, Vitamin Cs such as L-ascorbic acid, pantothenic acids such as calcium pantothenate, vitamin Ds such as ergocalciphenol, nicotinic acids such as nicotinamide, vitamin Es such as tocophenol acetate, vitamin P, biotin Etc.

Examples of the natural water-soluble polymer include gum arabic, tragacanth gum, galactan, guar gum, carob gum, karaya gum, carrageenan, pectin, agar, quince seed, algae colloid, starch,
Examples include xanthan gum, dextran, succinoglucan, pullulan, collagen, casein, hyaluronic acid, albumin and gelatin. Examples of the semi-synthetic water-soluble polymer include cellulosic polymers such as methylcellulose, nitrocellulose and sodium carboxymethylcellulose, starch-based polymers such as carboxymethylstarch, and alginic acid-based polymers such as sodium alginate. Examples of the synthetic water-soluble polymer include vinyl polymers such as polyvinyl alcohol and carboxyvinyl polymer, polyoxyethylene polymers such as polyethylene glycol 2000, and copolymer polymers such as polyoxyethylene polyoxypropylene copolymer. System, acrylic polymer such as polyacrylamide, polyethyleneimine, cationic polymer and the like.

Examples of the powder component include talc, kaolin, mica, sericite, magnesium carbonate, calcium carbonate, silicate, silica, barium sulfate, burnt gypsum, fluoroapatite, inorganic powder such as ceramic powder, nylon powder, and the like. Examples include organic powders such as polyethylene powder, polystyrene powder, and cellulose powder. As the coloring agent, inorganic pigments such as titanium dioxide, iron oxide, carbon black and cobalt violet, organic pigments such as red 201, red 3, yellow 205 and yellow 4, chlorophyll, riboflavin, β-carotene, etc. Natural pigments, plant extract pigments such as safflower and turmeric. Preservatives include benzoate, salicylate, sorbate, dehydroacetate, paraoxybenzoate, benzalkonium chloride, hinokitiol, resorcin, ethanol and the like. Examples of antioxidants include tocophenol, ascorbic acid, butylhydroxyanisole, dibutylhydroxytoluene, and gallic acid ester. Examples of the chelating agent include sodium ethylenediaminetetraacetate, sodium polyphosphate, citric acid and the like.

Furthermore, plant extracts having physiological activity such as antibacterial activity, cell activation, moisturizing, sebum secretion control, antiphlogistic, astringent, antioxidant, whitening, active oxygen suppression, antiallergic, etc., and their extracted fractions and purified products Can also be used together. Also,
In addition to the above, fragrance, alcohol, water and the like can be appropriately added.

The collagenase inhibitor of the present invention and the anti-aging cosmetics containing the same are not limited to general skin cosmetics but include pharmaceuticals, quasi drugs, cosmeceuticals and the like. is there. The dosage form of the skin external preparation composition of the present invention may be any of a solubilizing system, an emulsifying system, a powder dispersion system, and the like, and is also used for makeup such as lotions, emulsions, creams, basic cosmetics such as packs and foundations. Cosmetics, shampoo, conditioner, soap, toiletries such as body shampoo, and bath agents can be used.

Examples will be described below, but the present invention is not limited to these examples.

Test Example 1 Evaluation of Collagenase Inhibitory Activity It was measured by a method partially modified from the Wunsch-Heidrich method (Pharmaceutical Journal 118, 423-429, 1998). Collagenase is Type I manufactured by Sigma.
V is set to 5 mg / mL, and 100 μL of each is dispensed and stored frozen. At the time of use, it was diluted 50 times and used as 0.1 mg / mL. The collagenase synthetic substrate is a PZ-peptide (Pz
-Pro-Leu-Gly-Pro-D.Arg-O
H, manufactured by Bachem) 0.5 mg / mL.
The diluents were both 0.1M Tris buffer (pH 7.1,
20 mM CaCl ₂ was used). The test solution is
A 50 vol% ethanol solution was added to 50 g of the dried product of each plant, extracted at 50 ° C. for 8 hours, filtered, concentrated, and lyophilized, and then dissolved in purified water to 1 mg / mL.

(Test method) 400 μL of synthetic substrate, 50 μL of collagenase and 50 μL of test sample were added and reacted at 37 ° C. for 30 minutes, and then the reaction was stopped by adding 1 mL of 25 mM citric acid solution. After adding 5 mL of ethyl acetate and shaking vigorously, it was centrifuged at 3000 rpm. The ethyl acetate layer was taken and the absorbance was measured at 320 nm.

From the measurement results, the collagenase inhibition rate was calculated by the following formula. Collagenase inhibition rate (%) = [1- (AB) / (C-
D)] × 100 A: Absorbance when sample solution is added, collagenase is added B: Absorbance when sample solution is added, and collagenase is not added C: Sample is not added, absorbance when collagenase is added D: No sample is added, and collagenase is not added Absorbance However, when each was not added, purified water and buffer were used instead. Each inhibition rate is shown in Table 1.

[0035]

[Table 1]

Test Example 2 Skin Anti-aging Test In order to examine the anti-aging effect of the skin, the following Examples 1-5,
Using the cosmetics having the composition shown in Comparative Example 1, an evaluation test was performed for the effect of improving wrinkles and the effect of improving skin swelling and sagging by the following method.

Sixty randomly selected healthy women aged 40 to 50 years were used as subjects, and 12 of each of the cosmetics of Examples and Comparative Examples were used on the facial skin for 2 months every day. The effect of improving skin swelling and sagging was examined.

Example 1 Cream The following components (1) to (10), and separately the following components (11) to
(16) is dissolved by heating at 75 ° C. to prepare solutions A and B, respectively. Solution B is added to solution A to emulsify, stirring at 50 ° C
The mixture was cooled to 40 ° C. and ingredient (17) was added to prepare a cream. (Component) (% by weight) (1) Jojoba oil 3.0% (2) Squalane 2.0% (3) Methyl polysiloxane 0.5% (4) Stearyl alcohol 0.5% (5) Cetyl alcohol 0. 5% (6) Glyceryl tri (capryl / caprate) 12.5% (7) Glyceryl monostearate 5.0% (8) Diglyceryl monostearate 1.5% (9) Decaglyceryl monostearate 3. 0% (10) Propyl paraoxybenzoate 0.1% (11) Xanthan gum 0.1% (12) Spirea extract (Production Example 1) 3.0% (13) Glycerin 1.0% (14) 1,3 -Butylene glycol 5.0% (15) Methyl paraoxybenzoate 0.2% (16) Purified water 62.0% (17) Perfume 0.1%

(Examples 2 to 6) The mistletoe extract (Production Example 2) was used in place of the Spirea extract of Example 1 to obtain Example 2, and the jojoba leaf extract (Production Example 3) similarly. Was used as Example 3, the same lotus leaf extract (Production Example 4) was used as Example 4, and the same red clover extract (Production Example 5) was used as Example 5.

Comparative Example 1 Cream In Example 1,
A cream was prepared in the same manner as in Example 1 except that 3.0% of the Astragalus sinensis extract was replaced with 3.0% of purified water.

"Improvement Effect on Wrinkles" The state of wrinkles on the outer corners of the eyes was visually evaluated. (Judgment Criteria) Effective: Wrinkles were less noticeable Somewhat effective: Wrinkles were less noticeable than before No effect: No change "Improvement effect on skin swelling and sagging" Skin swelling and sagging were visually evaluated. (Judgment Criteria) Effective: There is more swelling on the skin than before use, and there is no slackness Somewhat effective: There is still some slackness on the skin compared to before use, there is no sagging effect No change: No change

[0042]

[Table 2]

As is clear from Table 2, when the creams of Examples 1 to 5 were used, the creases on the outer corners of the eyes, the stickiness of the skin, and the sagging were improved as compared with the case where the cream of Comparative Example 1 was used. It was recognized that it was done. From this, it was confirmed that the cosmetics containing the extract of Astragalus sinensis, the mistletoe extract, the jojoba leaf extract, the lotus leaf extract, and the red clover extract have an anti-aging effect.

The formulation examples of the present invention are shown below.

(Example 6) Toner Lotion The following components (5) to (8) were mixed and dissolved to prepare a liquid A, and separately, the following components (1) to (4) and (9) were mixed and dissolved to prepare a liquid B. As a liquid, the liquids A and B were evenly mixed to prepare a lotion. (Component) (wt%) (1) quince seed extract 8.0% (2) glycerin 3.0% (3) 1,3-butylene glycol 5.0% (4) Spirea extract (Production Example 1) 2 0.0% (5) Polyoxyethylene sorbitan lauric acid ester 1.2% (6) Ethyl alcohol 5.0% (7) Methyl paraoxybenzoate 0.2% (8) Perfume 0.1% (9) Purified water 75.5%

(Example 7) Emulsion The following components (1) to (10) and (11) to (14) and (16) were separately dissolved by heating at 75 ° C to prepare solutions A and B, respectively, Add Liquid B to emulsify and stir 5
It cooled to 0 degreeC and added the component (15), and prepared the emulsion. (Component) (% by weight) (1) Jojoba oil 1.0% (2) Squalane 2.0% (3) Behenyl alcohol 1.0% (4) Tri (capryl-capric acid) glyceryl 2.0% (5) Tetraglycerin condensed silinoleic acid 0.1% (6) Propylene glycol monooleate 0.5% (7) Glycerin monostearate 1.0% (8) Hexaglyceryl monomyristate 1.0% (9) Deca monomyristate Glyceryl 0.5% (10) Propyl paraoxybenzoate 0.1% (11) Quinceseed extract 5.0% (12) Mistletoe extract (Production Example 2) 2.0% (13) Jojoba leaf extract (Production) Example 3) 2.0% (14) 1,3-butylene glycol 3.0% (15) Perfume 0.1% (16) Purified water 78.7%

Example 8 Soap The following ingredients were mixed and produced by a standard method for producing soap.       (Component) (% by weight)   (1) Soap substrate 53.2%   (2) Scroll 19.4%   (3) Jojoba oil 0.25%   (4) Spirea extract (Production Example 1) 2.5%   (5) Jojoba leaf extract (Production Example 3) 2.5%   (6) Concentrated glycerin 6.5%   (7) Hydroxyethanediphosphonic acid 0.15%   (8) Constant water 15.5%

(Example 9) Cleansing gel The following components (1) to (3) and separately (4) to (6) and (8) were heated and dissolved at 70 ° C to prepare solutions A and B, respectively, and solution A was prepared. Solution B is added to and stirred until uniform. It cooled to 50 degreeC, stirring, and the ingredient (7) was added and the cleansing gel was manufactured. (Component) (% by weight) (1) Hexaglyceryl monomyristate 20.0% (2) Liquid paraffin 58.8% (3) Paraoxybenzoic acid ester 0.3% (4) Lotus leaf extract (Production Example 4) 0.5% (5) Red clover extract (Production Example 5) 0.5% (6) Concentrated glycerin 5.9% (7) Sorbitol 5.0% (8) Perfume 0.1% (9) Purified water 8.9%

Example 10 Packing Agent A phase, B phase, and C phase were uniformly dissolved, and phase B was added to phase A to solubilize it, and then phase C was added and dissolved uniformly,
To make. (Component) (% by weight) (Phase A) Dipropylene glycol 5.0% Polyoxyethylene hydrogenated castor oil 5.0% (Phase B) Olive oil 5.0% Tocophenol acetate 0.2% Paraoxybenzoic acid ester 2% (Phase C) sodium bisulfite 0.03% polyvinyl alcohol 13.0% jojoba leaf extract (Production Example 3) 1.0% red clover extract (Production Example 5) 1.0% ethanol 7.0% Purified water 62.77%

(Embodiment 11) Emulsion type foundation A is a powder part obtained by thoroughly mixing and pulverizing the following components (1) to (6), and (7) and (8) are solutions B, (9) to (12) and Let (14) be liquid C. After the solution C is heated and stirred, the solution A is added and subjected to a homomixer treatment, and then the solution B mixed by heating is added and subjected to a homomixer treatment. It is cooled to 50 ° C. with stirring, (13) is added, and further cooled to room temperature to manufacture. (Components) (% by weight) (1) Titanium dioxide 10.3% (2) Sericite 5.4% (3) Kaolin 3.0% (4) Yellow iron oxide 0.7% (5) Red iron oxide 0.4 % (6) Black iron oxide 0.2% (7) Decamethylcyclopentasiloxane 11.5% (8) Liquid paraffin 8.5% (9) Sorbitan sesquioleate 3.0% (10) Jojoba leaf extract (Production Example 3) 1.5% (11) 1,3-butylene glycol 5.0% (12) Paraoxybenzoic acid ester 0.2% (13) Perfume 0.2% (14) Purified water 50.1%

(Example 12) Solid foundation The following components (1) to (7) were uniformly mixed with a blender,
(8) to (14) are added to this and kneaded well to produce. (Component) (% by weight) (1) Talc 42.4% (2) Kaolin 15.5% (3) Sericite 10.0% (4) Zinc white 7.0% (5) Titanium dioxide 3.8% (6) Yellow iron oxide 2.9% (7) Black iron oxide 0.2% (8) Squalane 8.0% (9) Isostearic acid 4.0% (10) Polyoxyethylene sorbitan monooleate 3.0 % (11) Isocetyl octanoate 2.0% (12) Spirea extract (Production Example 1) 1.0% (13) Paraoxybenzoic acid ester 0.1% (14) Perfume 0.1%

The cosmetics of Examples 6 to 12 were all excellent in skin anti-aging effect.

[0053]

INDUSTRIAL APPLICABILITY As described above, the collagenase inhibitor and the anti-aging cosmetic composition of the present invention have an excellent collagenase inhibitory activity, prevent and improve skin aging, and have elasticity and wrinkle. You can maintain a youthful skin condition without sagging.

Front page continuation (51) Int.Cl. ⁷ identification code FI theme code (reference) A61K 7/00 A61K 7/00 U W 7/02 7/02 Z 7/48 7/48 7/50 7/50 A61P 17/16 A61P 17/16 43/00 111 43/00 111 F term (reference) 4C083 AA111 AA112 AB212 AB232 AB242 AB352 AB432 AB442 AC022 AC072 AC102 AC122 AC132 AC352 AC392 AC422 AC432 AC442 AC482 AC892 AD042 AD112 AD152 AD172 AD352 AD662 BB51 CC04 CC05 CC07 CC12 CC23 DD23 DD27 DD31 DD41 EE06 EE07 EE12 4C088 AB12 AB39 AB51 AB59 AC03 AC05 BA08 CA03 CA11 MA02 MA06 MA07 MA22 MA28 MA34 MA63 NA14 ZA89 ZB22 ZC20

Claims (2)

[Claims] 1. A collagenase inhibitor comprising, as an active ingredient, one or more solvent extracts of the group consisting of Astragalus sinensis, mistletoe, jojoba leaves, lotus leaves, and red clover. 2. An anti-aging cosmetic composition containing the collagenase inhibitor of claim 1.