JP2010043088A - Cosmetic product - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a cosmetic product compounded with a component having skin whitening action as well as skin aging preventing action, exhibiting a versatile and excellent preventing or ameliorating effect on symptoms of aged or unhealthy skin caused by internal and external factors such as wrinkles, sagging, spots and freckles by the synergistic effect of both actions to impart the skin with comprehensive skin-beautifying effect, almost free from skin stimulation, etc., and having excellent biological safety. <P>SOLUTION: The cosmetic product includes a fermentation product prepared by fermenting a plant of genus Nelumbo, family Nymphaeaceae with a microorganism such as lactobacillus. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to a cosmetic material that has both an excellent whitening effect and an anti-skin aging effect, imparts a comprehensive and multifaceted skin beautifying effect to the skin, and is excellent in biological safety.

Symptoms of aging and unhealthy skin, such as wrinkles, sagging, spots, and buckwheat, are caused by internal factors such as inactivation of cell proliferation and differentiation, decreased hormone secretion, and exposure to sunlight (ultraviolet rays). This is a phenomenon that is caused by complex intertwining of external factors such as cell / tissue damage, increased pigmentation, and onset of inflammation caused by active oxygen.
In order to prevent or improve this aging and unhealthy skin, and to keep the skin healthy and youthful, the use of various active ingredients has been proposed, and cosmetics containing these ingredients have been put on the market. Has been. For example, ascorbic acid and derivatives thereof, hydroquinone derivatives, kojic acid and derivatives thereof, whitening agents such as resorcinol derivatives, α-hydroxycarboxylic acids, placental extracts, cell activation components such as hormones, collagen, elastin, hyaluronic acid, etc. These include dermal matrix components, antioxidants such as vitamin E, anti-inflammatory agents such as glycyrrhetinic acid, various UV protection agents, and the like.
However, these conventional ingredients generally only prevent or ameliorate one of the above-mentioned factors of skin aging or unhealing, and depending on the cosmetics containing these ingredients, aging that can be truly satisfied It is difficult to obtain a prevention effect and a skin beautifying effect. In addition, some components may require improvement in terms of safety, for example, if the amount is increased in order to increase effectiveness, skin irritation may occur.

  In view of the problems of the prior art as described above, the present inventors keep the skin in a truly healthy and youthful state by acting in a complex manner on various factors such as aging and unhealthy skin. As a result of intensive research and investigation to find new active ingredients that can be improved in natural products from the viewpoint of biological safety, it can be obtained by fermenting a lotus plant of the genus Lilyaceae with microorganisms such as lactic acid bacteria. The fermented product has both a whitening effect based on a melanin production inhibitory action and a skin aging prevention effect based on a fibroblast activation action. The present invention has been completed by finding that the component can be imparted with a natural and healthy effect and that the component is derived from a natural product and thus has excellent biological safety.

Various proposals have been made for using lotus plants such as lotus (Nelumbo nucifera Gaertner) as cosmetic ingredients.
For example, Japanese Patent Laid-Open No. 3-190809 discloses an anti-skin improvement agent containing a lotus extract as an active ingredient, and Japanese Patent Laid-Open No. 4-247012 discloses a whitening cosmetic containing a lotus leaf extract. JP-A-11-279069, JP-A-2000-247829, JP-A-2001-122757, JP-A-2001-122757, JP-A-2002-29980, JP-A-2002-68993 and JP-A-2003-48846 The gazette discloses that components extracted from the whole lotus or specific site of the lotus are effective in preventing skin aging.
Furthermore, JP-A-8-20526 discloses that a bath preparation comprising an extract obtained by steaming lotus and then enzymatically degrading has a skin roughening effect and a whitening effect based on a melanin production inhibitory effect. Yes.

Japanese Patent Laid-Open No. 3-190809 JP-A-4-247010 JP-A-11-279069 JP 2000-247829 A JP 2001-122757 A JP 2001-122757 A JP 2002-29980 A JP 2002-68993 A JP 2003-48846 A JP-A-8-20526

However, in the case of these conventional techniques, the components contained in the lotus plant are used as they are, or simply hydrolyzed and used as a raw material for blending cosmetics. The present invention, which is used as an assimilation source and uses the fermentation product obtained here as a raw material for cosmetics, is clearly different from the technical idea. Therefore, naturally, these conventional techniques include the fermentation of the present invention. The product has both the whitening effect based on the suppression of tyrosinase activity in B16 melanoma cells and the effect of preventing skin aging by promoting the proliferation of human skin fibroblasts. Regarding what can be granted, there is no disclosure of the facts, or even an indication of this.
In addition, in the case of the prior art in which a lotus plant-containing component is used as it is or after being hydrolyzed, there is an aspect that it is difficult to say that the effectiveness that can be fully satisfied is still obtained, and the improvement effect on the skin This point is also inferior to the present invention (see the test examples described later).

That is, the present invention is a cosmetic comprising a fermented product obtained by fermenting a plant of the family Nympaeaceae (Nympaeaceae) with a microorganism.
Here, the term cosmetics is used in a broad sense including so-called cosmetics as well as quasi drugs.

The fermented lotus plant used as an active ingredient in the cosmetic composition of the present invention has both a melanin production-inhibiting action based on the inhibition of tyrosinase activity and a further significant activating action on dermal fibroblasts. Due to the synergistic effect based on the composite of the present invention, the cosmetic of the present invention exhibits a multifaceted and excellent effect in preventing or improving the symptoms of aging and unhealthy skin such as wrinkles, tarmi or stains and buckwheat, Maintain and improve the skin in a truly healthy and youthful state.
In addition, the active ingredient of the cosmetic of the present invention has almost no irritation to the skin, and therefore the cosmetic of the present invention is very excellent in biological safety.

Hereinafter, the present invention will be described in detail.
Examples of the plant belonging to the genus Lotusaceae used in the present invention include lotus (Nelumbo nucifera Gaertner) and American kivas (Nelumbo Lutea Pers.). Among them, the tyrosinase activity inhibitory action and fiber of the fermented product are mentioned. The use of lotus (Nelumbo nucifera Gaertner) is most preferable from the viewpoint of the blast cell proliferation promoting action.

  In fermenting these water lily family lotus plants, there is no particular limitation on the fermentation site of the plant, and appropriate parts such as whole grass, leaves, flowers, stamens, pistils, stems, rhizomes, seeds (grains) are added. Although it can be used, the use of seeds is preferable from the viewpoint of the effectiveness of the obtained fermented product, and among them, the use of lotus (Nelumbo nucifera Gaertner) seeds (grains) is most preferable.

Examples of microorganisms used for the fermentation of lotus plants include lactic acid bacteria, koji molds, natto bacteria, tempeh bacteria, and yeasts. Generally, one or more selected from any of these bacterial species are used. Depending on the conditions, those belonging to different bacterial species may be used in combination as long as they do not interfere with each other.
Among the above-mentioned bacterial species, particularly when lactic acid bacteria or koji molds are used, the obtained fermented product exhibits particularly strong tyrosinase activity suppressing action and fibroblast activation action, due to the synergistic and combined action of both these actions. In the present invention, it is particularly preferable to use one type or two or more types of bacteria selected from lactic acid bacteria and / or koji molds because excellent skin beautifying effects and skin sounding effects can be obtained.

  Examples of the lactic acid bacteria include Lactobacillus plantarum, L. brevis, Lactobacillus casei and other Lactobacillus lactic acid bacteria; Carnobacterium divergens Lactic acid bacteria of the genus Carnobacterium such as Carnobacterium piscicola; lactic acid bacteria of the genus Leuconostoc such as Leuconostoc mesenteroides and Leuconostoc citreum; Streptococcus faecalis, Streptococcus pyogenes and other Streptococcus lactic acid bacteria; Enterococcus caseliflavus, Enterococcus Lactic acid bacteria of the genus Enterococcus (Enterococcus) such as Sulfurus (Enterococcus sulfreus); Lactic acid bacteria of the genus Lactococcus rafinolactis such as Lactococcus rafinolactis; Weissella confusa lactic acid bacteria of the genus Weissela such as kandleri; lactic acid bacteria of the genus Atopobium such as Atopobium minutum, Atopobiumparvulus; Vagococcus fluvialis, Vagococcus moncocca V Pediococcus (Pediococcus damnosus), Pediococcus pentosaceus (Pediococcus pentosaceus), etc. ) Lactic acid bacteria of the genus. Among these lactic acid bacteria, the use of Lactobacillus plantarum is most preferable from the viewpoint of the skin physiological activity of the obtained fermented product and the fact that it is not extremely anaerobic and easy to handle.

Examples of Aspergillus oryzae include Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, Aspergillus sojae, Aspergillus solus, Aspergillus or kawauchii), Aspergillus usami,
Examples include black koji molds such as Aspergillus niger, Monascus anka, and Monascus pilosus. Among them, Aspergillus oryzae is most preferable from the viewpoint of the skin physiological activity of the obtained fermented product and the relatively low coloration and fermentation odor of the fermentation broth.

  Examples of Bacillus natto include bacteria belonging to the genus Bacillus such as Bacillus natto, Bacillus subtilis, and Bacillus circulans. Among these, Bacillus natto is most preferable because it is widely used in foods and has high safety.

  Tempe fungi include Rhizopus azygosporus, Rhizopus microsporus chinensis, Rhizopus microsporus oligosporus, Rhizopus microsporus oligosporus, Rhizopus microsporus oligosporus, Rhizopus microsporus oligosporus, Rhizopus microsporus oligosporus Examples include fungi. Among them, Rhizopus microsporus oligosporus and Rhizopus oryzae are most preferable because they are widely used for fermented foods in Southeast Asia including Indonesia.

Examples of the yeast include, for example, Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces cerevisiae, Torulaspora delbruekii, Torulaspora fermentati, Torulaspora rosei, etc.
omyces soya), Zygosaccharomyces sake, Zygosaccharomyces miso, Zygosaccharomyces lactis, etc. Kandida kefyr, Candida sake, Candida scottii and other yeasts of the genus Candida, Aureobasidium Pullulans, Aureobasidium mansonii, Aureobasidium Examples include yeast of the genus Aureobasidium such as Aureobasideium microstictum.
Among them, Saccharomyces cerevisiae is most preferable because it is most widely used for foods and has a strong fermenting power.

Preferable specific examples of the method of fermenting a lotus plant using the above microorganisms are as follows.
First, a lotus plant to be fermented (hereinafter sometimes referred to as a fermentation material) is immersed or suspended in a solvent to prepare a suspension for fermentation. In this case, the lotus plant may be used as it is, or may be used after being previously dried or semi-dried. In addition, as for the shape, the collected one can be used as it is, but the fermentation efficiency can be increased if it is shredded or pulverized and refined.
For example, when using lotus seeds (grains) as the fermentation material, it is preferable to remove the outermost astringent skin of the seeds in advance from the viewpoint of fermentation efficiency and hue of the fermented product obtained. It is preferable to use the crushed powder as a powder, rather than using the seed as it is, because it makes it easier for the lactic acid bacteria to use the nutrients.

As a solvent for suspending the fermentation material, water or water and lower alcohols (such as methanol, ethanol, propanol, etc.) or glycols (ethylene glycol, propylene glycol, 1,3-butylene glycol, glycerin, etc.) Mixtures etc. are used, and sugars such as glucose, fructose, sucrose may be added to these solvents, but the microbial is most likely to exert its action and assimilation components other than the lotus plant components Most preferably, water is used alone in order to avoid the production of fermentation by-products based on the presence of.
The mixing ratio of the fermentation material and the solvent is generally in the range of 1: 1 to 1: 1000, preferably 1: 5 to 1: 100, more preferably 1:10 to 1:50 in terms of dry weight of the fermentation material. .

Before the fermentation material / solvent suspension is subjected to a fermentation process, it is necessary to sterilize and remove the germs that hinder the fermentation. May be used by washing with ethanol for sterilization in advance and then suspending in a sterile solvent such as sterile water. After suspending the fermentation material in the solvent, the suspension is sterilized by heat sterilization or the like. May be.
As the heat sterilization treatment, the autoclave sterilization method in which the suspension is heated at 120 to 130 ° C. for 10 to 20 minutes, or holding at 80 to 90 ° C. for 60 to 120 minutes is repeated once a day for 2 to 3 days. The heat sterilization method such as the method is generally used.

Next, this sterilized suspension is placed in a fermentation tank, and microorganisms are inoculated therein to perform fermentation.
The appropriate amount of inoculated microorganism is 10 ⁷ to 10 ⁸ cells / mL. Even if the inoculation amount exceeds the above range, the fermentation progress time hardly changes. On the other hand, if the inoculation amount is less than the above range, it takes a long time to complete the fermentation.

  The fermentation temperature is generally in the range of 5 to 50 ° C., preferably in the range of 30 to 40 ° C. (for example, 35 ° C. to 40 ° C. for lactic acid bacteria), which is the optimum temperature for growth of each microorganism. The number of days of fermentation is generally in the range of 1 to 10 days, preferably 2 to 5 days, at the optimum temperature. If the fermentation days are shorter than the above general range, the fermentation is not sufficiently performed and the effectiveness of the fermented product tends to be reduced. On the other hand, if the fermentation days are longer than 10 days, no further increase in effectiveness is observed. Not only that, but also coloring and an increase in fermentation odor occur.

  In performing the above fermentation treatment, in order to more effectively utilize the components of the lotus plant by microorganisms, an enzyme is added to the suspension before or simultaneously with the inoculation of microorganisms, It is preferable to subject the lotus plant to a hydrolysis treatment with an enzyme, and this enzyme treatment is particularly suitable when a material having a dense outer skin and difficult to proceed fermentation, such as a lotus plant seed (for example, a lotus seed), is used. Especially effective.

  In this case, as the enzyme, at least one enzyme selected from a proteolytic enzyme, a starch degrading enzyme, a pectin degrading enzyme, and a fibrin degrading enzyme is used, and in particular, at least one selected from each of these four enzyme groups. Good results can be obtained by using one enzyme in combination.

  Examples of proteolytic enzymes include actinases such as actinase, pepsins such as pepsin, trypsins such as trypsin and chymotrypsin, papains such as papain and chymopapain, peptidases such as glycylglycine peptidase, carboxypeptidase and aminopeptidase. And bromelain can be used. Among these enzymes, actinases such as actinase, papains such as papain and chymopapain, and bromelain are particularly preferable.

  As the starch degrading enzyme, for example, α-amylase, β-amylase, glucoamylase, β-galactosidase and the like can be used. Of these enzymes, glucoamylase is particularly preferred.

  Examples of pectin degrading enzymes that can be used include pectin depolymerase, pectin demethoxylase, pectin lyase, pectin esterase, and polygalacturonase. Of these enzymes, pectin esterase and polygalacturonase are particularly preferred.

  Examples of the fibrinolytic enzyme include cellulase, hemicellulase, agarase, mannase, chitinase, chitosanase, carrageenase, arginase, fucoidanase, inulase, xylanase, and ligninase. Of these enzymes, cellulase, hemicellulase and ligninase are particularly preferred.

The total amount of the enzyme used is preferably 0.01 to 10% by weight, more preferably 0.1 to 1.0% by weight, based on the solid content of the lotus plant in the suspension.
As treatment conditions such as pH, temperature, and time, if the enzyme treatment is performed before fermentation, the treatment is preferably performed for 1 to 24 hours near the optimum pH and temperature of the enzyme used. If it is performed simultaneously with the fermentation, the same conditions as the fermentation may be used.

  When the above fermentation treatment is completed, the sterilization of the fermentation broth is performed at 70 to 100 ° C. for about 10 to 120 minutes for the sterilization of microorganisms or when the enzyme treatment is used in combination with the deactivation of the enzyme. After the treatment, the liquid phase is separated as it is, or generally and preferably by solid-liquid separation means such as filtration or centrifugation, and if necessary, the pH is adjusted to pH 6 to pH 6 which is the pH range of ordinary cosmetics. 8 is adjusted to an appropriate concentration by dilution or concentration, if necessary, and used as a cosmetic raw material. In some cases, the liquid phase after solid-liquid separation may be blended into a cosmetic material after being powdered according to a conventional method such as spray drying or freeze drying.

  Examples of cosmetics comprising the fermented lotus plant of the present invention include basic cosmetics such as emulsions, creams, lotions, essences, packs, facial cleansers, lipsticks, foundations, liquid foundations, makeup press powders, etc. Makeup cosmetics, facial cleansers, body shampoos, soaps and other cleaning cosmetics, and bath agents, but of course are not limited thereto.

  The blending amount of the fermented lotus plant in the cosmetic of the present invention is generally 0.001 to 10% by weight, preferably 0.01 to 1 in the case of a basic cosmetic, as the solid content of the fermented product. In the range of% by weight, in the case of makeup cosmetics, generally 0.001 to 5% by weight, preferably in the range of 0.01 to 0.5% by weight, and in the case of cleansing cosmetics, generally 0.001 to 5%. In the case of a bath agent, it is generally in the range of 0.001 to 10% by weight, preferably in the range of 0.01 to 1% by weight.

  In addition to the above-mentioned essential ingredients, the cosmetics of the present invention include compounding ingredients usually used in cosmetics, such as oily ingredients, surfactants, moisturizers, thickeners, antiseptic / disinfectants, powder ingredients, ultraviolet rays. Absorbers, pigments, fragrances, antioxidants and the like can be appropriately blended as necessary.

  Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty acid amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, etc. ON surfactant: quaternary ammonium salt, primary to tertiary fatty acid amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.
In addition, as emulsifiers or emulsifiers, stevia derivatives such as enzyme-treated stevia, lecithin and derivatives thereof, lactic acid bacteria fermented rice, lactic acid bacteria fermented rice, lactic acid bacteria fermented cereals (wheat, beans, millet, etc.), Zizyphus juazeiro: Rhamnaceae ) An extract or the like can also be blended.

  Examples of humectants include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate, and sugars such as trehalose, lactic acid bacteria fermented rice, and mucopolysaccharides. (For example, hyaluronic acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, hydrolyzed silk protein, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, phytosterol , Soybean phospholipid, cholesteryl isostearate, seaweed extract, seafood-derived collagen and its derivatives, various amino acids and their derivatives (eg trimethylglycine, etc.), beech Extract, soymilk fermented liquid, natto extract, rice-derived extract and fermented, and the like.

  Examples of thickeners include, for example, brown algae such as alginic acid, agar, carrageenan, fucoidan, green algae or red algae-derived components, beech extract, pectin, locust bean gum, polysaccharides such as aloe polysaccharide, xanthan gum, tragacanth gum, guar gum, etc. Synthetic polymers such as gums, cellulose derivatives such as carboxymethylcellulose, hydroxyethylcellulose, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives, polyglutamic acid and its derivatives, Examples thereof include sugar compounds mainly composed of glucosyl trehalose and hydrolyzed hydrogenated starch.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Examples thereof include Luconium, Salicylic acid, Ethanol, Undecylenic acid, Phenols, Jamal (Imidazodenyl urea), 1,2-pentanediol, various essential oils, bark dry product, propolis extract, methylisothiazolinone.

  Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, 6- or 12-nylon powder, polyethylene powder. Silk powder, cellulosic powder, grains (rice, wheat, corn, millet, etc.) powder, beans (soybean, red beans, etc.) powder, and the like.

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives, ubidecaquinone (ubiquinone), rutin, rutin glucoside, white coconut extract, rice extract, murasakikibu extract, birch extract Products, hamamelis extract, oolong tea extract, black bean hydrolyzed extract, hagoromogusa extract and the like.

  Further, if necessary, other active ingredients (whitening agent, skin aging prevention / roughness improving agent, etc.) may be blended within the range that does not impair the effects and features of the fermented product used in the present invention. For example, if it is a whitening agent, t-cycloamino acid derivative, kojic acid and its derivative, ascorbic acid and its derivative, hydroquinone derivative, ellagic acid and its derivative, resorcinol derivative, sucha akuhi extract, yukinoshita extract, rice bran extract, Rice bran extract hydrolyzate, lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented cereals (wheat, beans, millet), white coconut hydrolyzed extract, murasakixikib extract, lotus fruit fermented product, party extract, Pandanus amaryllifolius Roxb. Extract, Arcangelicia flava Merrilli extract, cami Vine extract (trade name: chamomile ET), ginkgo biloba extract, crested squirrel extract, itadori extract, squirrel foliage extract, licorice extract, dandelion extract, altea extract, geno shoko extract, yukinoshita extract, jujube extract , Peony extract, toki extract, peach extract, green algae, red algae or brown algae seaweed extract, seaweed extract such as sea eel, linoleic acid and its derivatives or processed products (eg liposomal linoleic acid) , 2,5-dihydroxybenzoic acid derivative, etc., if it is a component for preventing skin aging and improving rough skin, animal or fish-derived collagen and derivatives thereof, elastin and derivatives thereof, intercellular lipids such as ceramide, placenta extract, Nicotinic acid and its derivatives, glycyrrhizic acid and its derivatives (dipotassium salt, etc.), t-cycloamino Derivatives, vitamin A and derivatives thereof, vitamin E and derivatives thereof, allantoin, α-hydroxy acids, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, coenzyme Q-10, α-lipoic acid, picoline, gentian extract, Herbal extract such as licorice extract, pearl barley extract, chamomile extract, carrot extract, aloe extract, rice bran extract hydrolyzate, rice extract hydrolysate, low allergen rice extract hydrolysate, rice fermented extract, green algae, red Seaweed extract of algae or brown algae, seagrass extract such as sea cucumber, Sakuhakuhi extract, Zizyhus juazeiro extract, beech extract, Kidachi aloe extract, mannenrou extract, ginkgo extract, horsetail extract, safflower Extract, ginseng extract Elderberry extract, Hagoromogusa extract, Astragalus extract, Mango extract, Cherimoya extract, Mangosteen extract, Tabebuia impetiginosa extract, yeast extract, eggshell membrane extract protein, deoxyribonucleic acid potassium salt, lotus fermentation solution, purple orchid Root extract, murasakixikibu extract, rice extract, coral grass extract, pollen cargo extract and the like.

  Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, kojic acid sugar derivatives such as kojic acid glucoside, etc. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid), L-ascorbic acid-5-glucoside (5-O-α) -D-glucopyranosyl-L-ascorbine Acid) ascorbic acid sugar derivatives, acylated 6-positions of these ascorbic acid sugar derivatives (acyl groups are hexanoyl, octanoyl, decanoyl, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetra L-ascorbic acid tetrafatty acid esters such as lauric acid ester, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium and the like are hydroquinone derivatives such as arbutin (hydroquinone). -Β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside) and the like, and as resorcinol derivatives, for example, 4-n-butylresorcinol, 4-isoamylresorcinol and the like are 2,5-dihydroxybenzoic acid. Derivatives and For example, 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, etc., and nicotinic acid derivatives include, for example, nicotinic acid amide, nicotinic acid benzyl, etc. However, examples of vitamin E derivatives include vitamin E nicotinate, vitamin E linoleate, and sodium salt of vitamin E phosphate. Examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctane. There are acids.

  Next, the present invention will be described more specifically with reference to Production Examples, Examples (Cosmetics Formulation Examples) and Test Examples, but the present invention is not limited thereto. In the following, all parts are parts by weight, and all% are% by weight.

Production Example 1
100 g of lotus seeds (those with astringent skin removed) were pulverized, 1900 g of purified water was added to prepare a suspension, and heat sterilized. After adding 1 g of glucoamylase, 1 g of papain, and 0.5 g of cellulase to this suspension, 10 ⁸ cells / mL of lactic acid bacteria (Lactobacillus plantarum) were inoculated, and left to stand at 37 ° C. for 3 days in a nitrogen stream. . After culturing, the mixture was sterilized by heating, and the culture solution was filtered to obtain 1450 g of a lotus seed lactic acid bacteria fermented product solution (solid content concentration: 2.8%).

Production Example 2
In the same manner as in Production Example 1 except that Streptococcus faecalis was used instead of Lactobacillus plantarum as lactic acid bacteria, 1420 g of a lactic acid bacteria fermented solution of lotus seeds (solid content concentration 2.5%) was obtained.

Production Example 3
In the same manner as in Production Example 1, except that Lactobacillus plantarum was used instead of Lactobacillus plantarum, 1380 g of a lotus seed lactic acid bacterium fermentation product solution (solid content concentration 2.6%) was obtained.

Production Example 4
In the same manner as in Production Example 1, except that Lactobacillus plantarum was used in place of Lactobacillus plantarum as lactic acid bacteria, 1440 g of a lotus seed lactic acid bacteria fermented solution (solid content concentration 2.3%) was obtained.

Production Example 5
In the same manner as in Production Example 1 except that Lactobacillus plantarum was used in place of Lactobacillus plantarum as a lactic acid bacterium, 1410 g of a fermented lactic acid bacterium solution (solid content concentration 2.3%) was obtained.

Production Example 6
A lotus seed fermented lactic acid bacteria solution 1440 g (solid content concentration 2.7%) was obtained in the same manner as in Production Example 1 except that the number of culture days was 5 days. .

Production Example 7
In the same manner as in Production Example 1, except that a lotus leaf shredded product is used in place of the lotus seed pulverized material as a fermentation material, 850 g of a lotus leaf lactic acid bacteria fermented solution (solid content concentration 1.1%) Obtained.

Production Example 8
In the same manner as in Production Example 1 except that shredded lotus rhizome is used in place of ground lotus seed as a fermentation material, 1050 g of lactic acid bacteria fermented solution of lotus rhizome (solid content concentration 1.8%) Obtained.

Production Example 9
1150 g of lactic acid bacteria fermented solution of whole lotus grass (solid content concentration 1.3%) )

Production Example 10
100 g of lotus seeds (those with astringent skin removed) were pulverized, 1900 g of purified water was added to prepare a suspension, and heat sterilized. This suspension was inoculated with 10 ⁸ cells / mL of lactic acid bacteria (Lactobacillus plantarum) and allowed to stand at 37 ° C. for 3 days under a nitrogen stream. After completion of the culture, the mixture was sterilized by heating, and the culture solution was filtered to obtain 1250 g of a lotus seed lactic acid bacteria fermented product solution (solid content concentration 1.2%).

Production Example 11
1150 g of a lactic acid bacteria fermented solution of American Kibas seeds (solids concentration: 2. 5%).

Production Example 12.
A lotus seed lactic acid bacteria fermentation product solution 1000 g obtained in the same manner as in Production Example 1 was freeze-dried and pulverized to obtain a lotus seed lactic acid bacteria fermentation product powder 26 g.

Production Example 13
In the same manner as in Production Example 1, except that lactic acid bacteria (Lactobacillus plantarum) was used instead of lactic acid bacteria (Lactobacillus plantarum), 1400 g (solid content concentration: 3.1%) of a lotus seed gonococcal fermented solution was obtained. .

Production Example 14
In the same manner as in Production Example 13, except that Aspergillus oryzae was used in place of Aspergillus oryzae as a koji mold, 1430 g of a lotus seed koji mold fermented product solution (solid content concentration: 3.0%) was obtained.

Production Example 15.
In the same manner as in Production Example 1, except that lactic acid bacteria (Lactobacillus plantarum) are used instead of lactic acid bacteria (Lactobacillus plantarum), 1410 g of fermented natto bacteria fermented solution of lotus seeds (solid concentration 3.2%) Obtained.

Production Example 16.
In the same manner as in Production Example 15 except that Bacillus subtilis was used in place of Bacillus natto as Bacillus natto, 1440 g of a fermented fermented natto bacterium (solid content concentration: 3.0%) was obtained.

Production Example 17
As a microorganism, 1410 g of a yeast fermented yeast solution (solid content concentration 2.2%) was obtained in the same manner as in Production Example 1 except that Saccharomyces cerevisiae, which is yeast, was used instead of lactic acid bacteria (Lactobacillus plantarum).

Production Example 18.
In the same manner as in Production Example 17, except that Saccharomyces cerevisiae was used in place of Saccharomyces cerevisiae, 1400 g of a fermented lotus seed yeast solution (solids concentration 2.4%) was obtained.

Production Example 19.
As in Production Example 1, except that lactic acid bacteria (Lactobacillus plantarum) are used as microorganisms and Aspergillus oryzae of Aspergillus is used instead of lotus seeds as fermentation material, American Kibas seeds are used. 1410 g (solid content concentration 3.1%) of a koji mold fermented product was obtained.

Production Example 20.
In the same manner as in Production Example 1, except that lactic acid bacteria (Lactobacillus plantarum) are used instead of lactic acid bacteria (Lactobacillus plantarum), 1420 g (solid content concentration 3.2%) of lotus seed fermented tempe bacteria solution is the same as in Production Example 1. Obtained.

Production Example 21.
A lotus seed fermented tempe fungus solution 1450 g (solid content concentration 3.1%) was obtained in the same manner as in Production Example 20 except that Rhizopus microporus oligospora was used instead of Rhizopus microporus oligospora.

Comparative Production Example 1
A lotus seed (with astringent skin removed) (100 g) was pulverized, 1900 g of purified water was added to prepare a suspension, and the mixture was heated at 80 ° C. for 2 hours. This solution was filtered to obtain 1360 g of a lotus seed extract solution (solid content concentration: 0.7%).

Comparative Production Example 2
100 g of lotus seeds (those with astringent skin removed) were pulverized, 1900 g of purified water was added to prepare a suspension, and heat sterilized. After adding 1.0 g of papain to this solution, the pH was adjusted to 7.5 and kept at 45 ° C. for 15 hours. This solution was filtered to obtain 1410 g of a lotus seed enzyme degradation product solution (solid content concentration 2.1%).

Example 1. Cream [Component A] Liquid paraffin 5.0
Hexalan (Note 1) 4.0
Paraffin 5.0
Glyceryl monostearate 2.0
Polyoxyethylene (20) sorbitan monostearate 6.0
Butylparaben 0.1
(Note 1) Technoble Co., Ltd. glyceryl trioctanoate
[B component]
Lotus seed fermented product solution of Production Example 1 10.0
Glycerin 5.0
Carboxymethyl monostearate 0.1
Moiston C (Note 2) 1.0
Amount of purified water totaling 100 parts (Note 2) NMF component manufactured by Technoble Co., Ltd.
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After this was cooled to 50 ° C., component C was added and further stirred and mixed to obtain a cream.

Example 2 Cream A cream was obtained in the same manner as in Example 1 except that the lotus seed fermented product solution of Production Example 6 was used instead of the lotus seed fermented product solution of Production Example 1 in the component B of Example 1.

Example 3 FIG. Cream A cream was obtained in the same manner as in Example 1 except that the lotus seed fermented product solution of Production Example 13 was used instead of the lotus seed fermented product solution of Production Example 1 in the component B of Example 1.

Example 4 Cream A cream was obtained in the same manner as in Example 1 except that the lotus seed fermented product solution of Production Example 15 was used instead of the lotus seed fermented product solution of Production Example 1 in the component B of Example 1.

Embodiment 5 FIG. Cream A cream was obtained in the same manner as in Example 1 except that the lotus seed fermented product solution of Production Example 17 was used instead of the lotus seed fermented product solution of Production Example 1 in the component B of Example 1.

Example 6 Cream A cream was obtained in the same manner as in Example 1 except that the lotus seed fermented product solution of Production Example 19 was used instead of the lotus seed fermented product solution of Production Example 1 in the component B of Example 1.

Example 7 Cream A cream was obtained in the same manner as in Example 1 except that the lotus seed fermented product solution of Production Example 1 was used instead of the lotus seed fermented product solution of Production Example 1 in the component B of Example 1.

Example 8 FIG. Emulsion [component A] part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Lotus seed fermented product solution of Production Example 10.0 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 9 Emulsion An emulsion was obtained in the same manner as in Example 8 except that the lotus seed fermented product solution of Production Example 3 was used instead of the lotus seed fermented product solution of Production Example 2 in the component B of Example 8.

Example 10 Emulsion An emulsion was obtained in the same manner as in Example 8 except that the lotus seed fermented product solution of Production Example 4 was used instead of the lotus seed fermented product solution of Production Example 2 in the component B of Example 8.

Example 11 Emulsion An emulsion was obtained in the same manner as in Example 8 except that the lotus seed fermented product solution of Production Example 5 was used instead of the lotus seed fermented product solution of Production Example 2 in the component B of Example 8.

Example 12 FIG. Emulsion An emulsion was obtained in the same manner as in Example 8, except that the lotus leaf fermented product solution of Production Example 7 was used instead of the lotus seed fermented product solution of Production Example 2 in Component B of Example 8.

Example 13 Emulsion An emulsion was obtained in the same manner as in Example 3 except that the lotus rhizome fermented product solution of Production Example 8 was used instead of the lotus seed fermented product solution of Production Example 2 in the component B of Example 8.

Example 14 Emulsion An emulsion was obtained in the same manner as in Example 8 except that the lotus seed fermented product solution of Production Example 10 was used instead of the lotus seed fermented product solution of Production Example 2 in the component B of Example 8.

Example 15. Emulsion An emulsion was obtained in the same manner as in Example 8 except that the lotus seed fermented product solution of Production Example 14 was used instead of the lotus seed fermented product solution of Production Example 2 in Component B of Example 8.

Example 16 Emulsion An emulsion was obtained in the same manner as in Example 8, except that the lotus seed fermented product solution of Production Example 2 was used instead of the lotus seed fermented product solution of Production Example 2 in Example 8.

Example 17. Emulsion An emulsion was obtained in the same manner as in Example 8 except that the lotus seed fermented product solution of Production Example 18 was used instead of the lotus seed fermented product solution of Production Example 2 in the component B of Example 8.

Example 18 Emulsion An emulsion was obtained in the same manner as in Example 8 except that the lotus seed fermented product solution of Production Example 20 was used instead of the lotus seed fermented product solution of Production Example 2 in the component B of Example 8.

Example 19. Lotion [ingredient] part Fermented product solution of whole lotus of production example 9 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total
The above ingredients were mixed to obtain a lotion.

Example 20. Lotion [component A] part olive oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Butylparaben 0.1
[B component]
Lotus seed fermented product solution of Production Example 11 10.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total
[C component]
Perfume
After each component A and component B was heated to 80 ° C. or higher, the component B was added to the component A and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes.
After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Example 21. Emulsion [component A] part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Lotus seed fermented product solution of Production Example 1 10.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 22. Latex In addition to using 2.0 parts of L-ascorbic acid-2-phosphate magnesium in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the component B of Example 21 Gave an emulsion in the same manner as in Example 21.

Example 23. Emulsion In addition to using 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the component B of Example 21, 2.0 parts of sodium L-ascorbic acid-2-phosphate is used. Gave an emulsion in the same manner as in Example 21.

Example 24. Emulsion In the component B of Example 21, the emulsion was prepared in the same manner as in Example 21 except that 2.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. Obtained.

Example 25. Latex In the component B of Example 21, in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide, a rice bran extract hydrolyzate (trade name “Grace Snow” manufactured by Technoble Co., Ltd.) An emulsion was obtained in the same manner as in Example 21 except that 5.0 parts of * Snow * HP ", solid concentration 3.5%) were used.

Example 26. FIG. Emulsion White coconut extract (trade name “Sinablanca-WH” manufactured by Technoble Co., Ltd.) instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the component B of Example 21 , Solid content concentration 1.0%) An emulsion was obtained in the same manner as in Example 21 except that 5.0 parts were used.

Example 27. Emulsion Example 21 except for using 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the component B of Example 21 except that 1.0 part of γ-amino-β-hydroxybutyric acid is used. In the same manner as in No. 21, an emulsion was obtained.

Example 28. Emulsion [component A] part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Lotus seed fermented product solution of Production Example 10.0 10.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Coenzyme Q-10 0.1
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 29. Emulsion [component A] part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Lotus seed fermented product solution of Production Example 10.0 10.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Soy milk lactic acid bacteria fermentation extract 1.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 30. FIG. Pressed powder [A component] Part Bengala 0.5
Yellow iron oxide 1.5
Black iron oxide 0.1
Titanium oxide 10.0
Nylon powder 4.0
Amount that makes 100 parts of sericite Mica 23.0
Talc 25.0
Lotus seed fermented product powder of Production Example 12 2.0
[B component]
Squalane 1.0
Methylpolysiloxane 4.0
Propylparaben 0.1
Dehydroacetic acid 0.1
Liquid paraffin 2.0
Perfume
The above A component and B component were mixed and agitated, mixed, then passed through a 200 mesh Tyler mesh sieve, and the resulting mixed powder was cast into a mold to obtain a pressed powder.

Example 31. Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[B component]
Lotus seed fermented product solution of Production Example 1 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [C component]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Example 32. Cream foundation [component A] part Stearic acid 5.0
Cetanol 2.0
Glyceryl monostearate 3.0
Liquid paraffin 5.0
Squalane 3.0
Isopropyl myristate 8.0
Polyoxyethylene (20) glyceryl monostearate 2.0
Propylparaben 0.1
[B component]
Lotus seed fermented product solution of Production Example 2 5.0
Sorbitol 3.0
1,3-butylene glycol 5.0
Triethanolamine 1.5
Methylparaben 0.1
Purified water Amount of 100 parts [component C]
Titanium oxide 8.0
Talc 2.0
Kaolin 5.0
Bentonite 1.0
Coloring pigment appropriate amount [component D]
Fragrance 0.3
Component C was mixed and pulverized with a pulverizer. The component B was mixed, and the pulverized component C was added thereto and uniformly dispersed in a colloid mill. The components A and B and C dispersed uniformly were each heated to 80 ° C., and then the components A were added to the components B and C while stirring, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, D component was added and stirred and mixed, and also it cooled to 30 degrees C or less, stirring, and obtained the cream foundation.

Example 33. Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[B component]
Lotus seed fermented product solution of Production Example 4 10.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Example 34. Soap [component A] part hardened castor oil 26.0
Coconut oil 10.0
Olive oil 4.0
[B component]
Sodium hydroxide 6.0
Sugar 10.0
Glycerin 5.0
Lotus seed fermented product solution of Production Example 5 5.0
Purified water Amount of 100 parts [component C]
Ethanol 20.0
Perfume Appropriate A component and B component were each heated to 80 ° C. and dissolved uniformly, and then B component was added to A component to saponify. This was cooled to 50 ° C. with stirring, and component C was added. This was poured into a mold and cooled, and then dried at room temperature for several days. A sufficiently dried product was taken out from the mold to obtain soap.

Comparative Example 1 Cream A cream was obtained in the same manner as in Example 1 except that the lotus seed extract solution of Comparative Production Example 1 was used instead of the lotus seed fermented product solution of Production Example 1 in the component B of Example 1.

Comparative Example 2 Cream A cream was obtained in the same manner as in Example 1, except that the lotus seed fermented product solution of Production Example 1 was used instead of the lotus seed fermented product solution of Comparative Production Example 2.

Test Example 1 Inhibition of intracellular tyrosinase activity (1)
Using the lotus seed fermented product solutions of Production Example 1, Production Example 2 and Production Example 3 as samples, the relationship between the type of lactic acid bacteria and the tyrosinase inhibitory action of the obtained fermentation product was examined.

[Test method]
Cultured B16 mouse melanoma cells were seeded at 8 × 10 ³ cells / well in a 96-well microplate, and were subjected to 1 under conditions of 37 ° C. and 5% CO 2 in Eagle's minimum essential medium (MEM) containing 10% calf serum (FBS). After pre-culturing for a day, the sample solution was replaced with a solution diluted with 2.5% or 5.0% concentration (as a solution) with 10% FBS-containing Eagle MEM, and cultured under the same conditions for 2 days.
Next, after removing the culture solution, adding a surfactant (Triton X-100) and 5 mM L-dopa solution and reacting at 37 ° C., using a microplate reader (Model 450, manufactured by Bio-Rad), The dopa value was measured at a wavelength of 490 nm.
In the case of no sample addition (control), the same operation as described above was performed, and the relative value of the dopa value at the time of each sample addition with respect to the obtained dopa value was determined and used as the tyrosinase activity rate (%).
For comparison, the same test was performed when 3 mM arbutin was added instead of the sample solution (positive control).

[result]
The results obtained in the above test are shown in Table 1.

As shown in Table 1, the fermented product solution fermented with any lactic acid bacterium suppressed tyrosinase activity. Among them, the fermented product solution obtained using Lactobacillus plantarum showed the strongest tyrosinase activity suppressing action. It was.
Further, the lotus seed fermented product solutions obtained in Production Examples 4 and 5 were tested in the same manner as described above. As a result, it was found that both had tyrosinase inhibitory action substantially equivalent to Production Examples 2 and 3.
Note that arbutin used as a positive control in this test also markedly inhibits tyrosinase activity, indicating that the test system was normal.

Test Example 2 Inhibition of intracellular tyrosinase activity (2)
Production Example 1 (lotus seed fermented solution), Production Example 7 (lotus leaf fermented solution), Production Example 8 (lotus rhizome fermented solution) and Production Example 11 (American kibas seed fermented) Each fermented product solution of the product solution was used as a sample, and the relationship between the site or type of the lotus plant and the tyrosinase inhibitory effect of the obtained fermented product was examined by the same method as in Test Example 1.
Arbutin was used as a positive control.

[result]
The results obtained in the above test are shown in Table 2.

  As shown in Table 2, the fermented solution obtained suppressed the tyrosinase activity when any part of the lotus was used as the fermentation material or when the seeds of American kibas were used. The fermented product solution using lotus seeds showed the strongest tyrosinase activity inhibitory action.

Test Example 3 Inhibition of intracellular tyrosinase activity (3)
Production Example 1 (lotus seed fermented lactic acid bacteria solution), Production Example 13 (lotus seed bacilli fermented fermented solution), Production Example 15 (lotus seed fermented natto bacteria fermented solution), Production Example 17 (lotus seeds) Yeast fermentation solution) and production example 20 (lotus fungus fermented solution of lotus seed) as samples, and using the same method as in Test Example 1, the types of microorganisms and the tyrosinase activity of the obtained fermentation product The relationship with the inhibitory effect was investigated. However, the concentration of the sample solution was 5.0%.
Arbutin was used as a positive control.

[result]
The results obtained in the above test are shown in Table 3.

  From the results of Table 3, the lotus seed fermented product obtained using any of the microorganisms of lactic acid bacteria, koji molds, natto bacteria, yeasts, and tempeh bacteria also has an action of suppressing B16 intracellular tyrosinase activity. However, it can be seen that the fermented product produced by lactic acid bacteria or koji molds exhibits excellent inhibitory action.

Test Example 4 Inhibition of intracellular tyrosinase activity (4)
Using the same method as in Test Example 1, the tyrosinase inhibitory action of the lotus seed fermented product solution of Production Example 1 (example of the present invention) and the lotus seed extract solution of Comparative Production Example 1 and the lotus seed enzymatic degradation of Comparative Production Example 2 The tyrosinase inhibitory action of the product solution was compared. Arbutin was used as a positive control.

[result]
The results obtained in the above test are shown in Table 4.

  As shown in Table 4, the lotus seed fermented product solution of Production Example 1 (Example of the present invention) showed a strong tyrosinase activity inhibitory action, but the lotus seed extract solution of Comparative Production Example 1 and the lotus seed enzyme of Comparative Production Example 2 The degradation product solution hardly showed tyrosinase activity inhibitory action.

Test Example 5 Fibroblast activation test (1)
About the sample similar to Experiment 1, the relationship between the kind of lactic acid bacteria and the fibroblast activation effect | action of the obtained fermented material was investigated.

[Test method]
Human dermis-derived fibroblasts NB1RGB (000824) were seeded at 1 × 10 ⁴ cells / well in a 96-well microplate containing 0.5% FCS-containing minimum essential medium, pre-cultured at 37 ° C. for 1 day, The sample solution was added to a concentration of 2.5% or 5.0%, and further cultured at 37 ° C. for 6 days. Next, 0.2% MTT was added to the cell treatment solution to which the medium was removed and the surfactant (TRITON X-100) was added, and maintained at 37 ° C., and then a microplate reader (Model 1450, Bio-Rad). MTT value was measured at a wavelength of 370-630 nm.
In the case where no sample was added (control), the same operation as described above was performed, and the relative value of the MTT value at the time of adding each sample to the MTT value obtained here was determined to obtain the fibroblast MTT activity rate (%). .
For comparison, the same test was performed when 50 mM glucose was added instead of the sample solution (positive control).

[result]
The results are shown in Table 5.

As shown in Table 5, the fermented product solution fermented with any lactic acid bacterium showed a fibroblast activation effect. Among them, the fermented product solution obtained using Lactobacillus plantarum had the strongest fibroblasts. The activation effect was shown.
Moreover, when the same test as above was carried out on the lotus seed fermented product solutions obtained in Production Examples 4 and 5, it was found that both had fibroblast activation effects substantially the same as Production Examples 2 and 3. did.

Test Example 6. Fibroblast activation test (2)
Using the same sample as in Test Example 2, the relationship between the lotus plant part or type and the fibroblast activation effect of the obtained fermented product was examined in the same manner as in Test Example 5.

[result]
The results are shown in Table 6.

  As shown in Table 6, both of the obtained fermented product solutions exhibited a fibroblast activation effect when any part of the lotus was used as a fermentation material or when American Kibas seeds were used. Among them, the fermented product solution using lotus seeds showed the strongest fibroblast activation effect.

Test Example 7 Fibroblast activation test (3)
Using the same sample as in Test Example 3, the relationship between the type of microorganism and the fibroblast activation effect of the obtained fermented product was examined by the same method as in Test Example 5.

[result]
The results are shown in Table 7.

  As shown in Table 7, the obtained lotus seed fermented product has the effect of activating fibroblasts when using any of the microorganisms of lactic acid bacteria, koji molds, natto bacteria, yeasts, and tempeh bacteria. The activation is most prominent when using.

Test Example 8. Fibroblast activation test (4)
By using the same sample as in Test Example 4, the same method as in Test Example 5 was used to compare the fibroblast activation effect of the lotus plant fermented product of the present invention with a conventionally known lotus plant-derived component.

[result]
The results are shown in Table 8.

  As shown in Table 8, the lotus seed fermented product solution of Production Example 1 (example of the present invention) showed a strong fibroblast activation effect, but the lotus seed extract solution of Comparative Production Example 1 and the lotus seed of Comparative Production Example 2 The enzyme degradation product solution showed almost no fibroblast activation effect.

Test Example 9 Skin primary irritation test Using a guinea pig, the skin primary irritation of the lotus plant fermented product of the present invention was examined.
[Test method]
Three Hartley guinea pigs (male, 4 weeks old) (GA, GB and GC) were used to remove their hair with clippers and an electric shaver. ) Was pasted with the fermented product solution of Production Example 1 or Production Example 9, or 0.5 mL of purified water wetted as a control. The adhesive bandage is removed 24 hours after the start of application, and immediately after the removal (24 hours after the start of application), 24 hours after the removal (48 hours after the start of application) and 48 hours after the removal (72 hours after the start of application). The degree of erythema, crust and edema formation at the applied site was observed and evaluated according to the following criteria for Draize.

(Erythema)
Score Skin condition 0: No erythema 1: Extremely mild erythema 2: Clear erythema 3: Moderate to strong erythema 4: Light crust formation in strong crimson erythema (edema)
Score Skin condition 0: No edema 1: Extremely mild edema 2: Clear edema (clearly distinguishable from surroundings)
3: Moderate edema (swelling of 1 mm or more)
4: Strong edema (further spread around)

[result]
The results are shown in Table 9.

  As is clear from the results in Table 9, the lotus fermented product used as a cosmetic ingredient in the present invention has no primary irritation to the skin and is extremely excellent in biological safety.

Test Example 10 Monitor test The effects of the cream of Example 1 and the creams of Comparative Examples 1 and 2 on the skin were examined by a monitor test.
[Test method]
Randomly extracted 40 women aged 18-50 were divided into two groups (A, B) of 20 subjects each, and each group was divided into two types of creams of Example 1 and Comparative Example 1 or 2. After assigning one of the combinations and applying the cream of the example or comparative example twice a day (morning and evening) for 1 month to the left and right cheeks respectively, an improvement effect on spots and freckles, small The improvement effect on wrinkles and the improvement effect on skin elasticity and gloss were evaluated based on the following evaluation criteria.

[Evaluation criteria]
(Improvement effect on spots and freckles)
A: Very improved B: Much improved C: Slightly improved D: No change E: On the contrary, it became more conspicuous (improvement effect on small wrinkles)
A: Almost inconspicuous B: Almost inconspicuous C: Slightly inconspicuous D: Unchanged E: On the contrary (Improvement effect on skin elasticity and gloss)
A: Clearly improved B: Much improved C: Slightly improved D: No change E: On the contrary

[result]
The results are shown in Table 10. In addition, the number of each evaluation column of AE of Table 10 shows the number of the test subjects who performed the said evaluation in 20 test subjects.

As shown in Table 10, the cream of the present invention comprising a lotus seed fermented product as an active ingredient has a synergistic effect based on the combination of the melanin production inhibitory action and the fibroblast activation action of the ingredient, thereby aging the skin. Alternatively, it exhibits an excellent ameliorating action for any of the typical symptoms of unhealthy spots such as stains, buckwheat, wrinkles and sagging.
In contrast, the cream containing the conventional lotus seed extract or lotus seed enzyme degradation product showed almost no improvement effect, which clearly showed a difference in effectiveness.

Claims (9)

A cosmetic comprising a fermented product obtained by fermenting a plant of the genus Nympaeaceae (Nympaeaceae) with a microorganism. The cosmetic according to claim 1, wherein the plant of the genus Lotusaceae is lotus (Nelumbo nucifera Gaertner) or American kibas (Nelumbo lutea pers). The cosmetic according to claim 1 or 2, wherein seeds of the genus Lotusaceae are used. The cosmetic according to claim 1, wherein the microorganism used for fermentation is selected from lactic acid bacteria, koji molds, natto bacteria, tempeh bacteria, and yeast. The cosmetic according to claim 4, wherein the microorganism used for fermentation is selected from lactic acid bacteria and koji molds. The cosmetic according to claim 4 or 5, wherein Lactobacillus plantarum (Lactobacillus pl antrum) is used as the lactic acid bacterium. The cosmetic according to claim 4 or 5, wherein Aspergillus oryzae is used as the koji mold. The water lily family Lotus plant is hydrolyzed with at least one enzyme selected from a proteolytic enzyme, a amylolytic enzyme, a pectin degrading enzyme and a fibrinolytic enzyme before and / or during the fermentation of lactic acid bacteria. Cosmetics described in 1. The cosmetic according to claim 8, wherein four kinds of enzymes, a proteolytic enzyme, a starch degrading enzyme, a pectin degrading enzyme and a fibrin degrading enzyme, are used in combination.