JP2005239660A - Precursor lipocyte differentiation promoter - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a precursor lipocyte differentiation promoter having decomposability and locally accumulating fat to a part difficult to cause the fat accumulation. <P>SOLUTION: One or more kinds of extracts of plants selected from Citrus unshiu, Dioscorea tokoro, Diospyros kaki, Cinnamomum cassia, Paeonia lactiflora, Cnidium officinale, Polygonum multiflorum, Eriobotrya japonica and Actinidia polygama are used as a precursor lipocyte differentiation promoter. The extract is used as it is or in combination with other components as a food, drink, internal medicine, skin care preparation for external use, cosmetic, bathing agent, animal feed, or the like. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a preadipocyte differentiation promoter useful as a fat accumulation promoter, health enhancer, beauty agent, livestock feed additive and the like.

  With the recent increase in interest in health and beauty, an increasing number of people want to make their bodies healthy and attractive regardless of gender.

  Among them, there are very many people who want femininity such as a female bust, and do cosmetic surgery such as fat injection, silicone injection, esthetic salon treatment, supplement or health food intake. ing.

  However, the effects obtained by the methods, drugs, supplements, etc. proposed so far vary greatly from individual to individual, and no effect has been found. Furthermore, treatments in cosmetic plastic surgery and esthetic salons are very expensive and there are concerns over adverse effects over time (deterioration of skin quality, shape loss, etc.).

  It is known that 90% of female breasts are composed of adipose tissue (cells). As most women experience, when weight (body fat) decreases due to various factors such as diet and stress due to dietary restrictions, breasts first become smaller. On the other hand, when body weight (body fat) increases due to excess calorie intake, fat accumulates in the face, stomach, thighs, or internal organs, but the breast does not change much. In other words, it is the amount and quality of fat stored in adipose tissue (adipocytes) that characterizes the size and shape of a woman's breast, and it can be said that the fat is very easily decomposed and difficult to accumulate. .

  An object of the present invention is to provide a preadipocyte differentiation promoting agent that enables efficient local accumulation of fat (adipose tissue) at a site that is very easily decomposed and difficult to accumulate. In addition, the present invention applies the preadipocyte differentiation promoter, a fat accumulation promoter for health promotion, a breast augmentation agent for women who want femininity, a cosmetic additive that gives skin tension, Provide animal feed additives.

  The present inventors pay attention to the fact that fat cells having the ability to accumulate fat are differentiated from preadipocytes, and it is considered that promoting this differentiation is important for fat accumulation. We have earnestly studied on differentiation. As a result, it was found that a specific crude drug promotes differentiation of preadipocytes, and this finding has led to the completion of the present invention.

  That is, the present invention provides a preadipocyte differentiation promoter containing one or more of plant extracts selected from Satsuma mandarin, Onidokoro, Oyster, Kay, Peonies, Senkyu, Tsurugukudami, Loquat, and Matabi. .

  Extracts of Satsuma mandarin, Onidokoro, Oyster, Kay, Peonies, Senkyu, Tsurugukudami, Biwa, and Matabi are useful because they have the effect of promoting the differentiation of preadipocytes. These are formulated into cosmetics and other skin external preparations, bath preparations, and food and drink to promote the accumulation of local or whole body neutral fat, enriching the breasts for women who want femininity, Can give skin tension. Furthermore, application to the livestock field including livestock feed is also possible.

  The preadipocyte differentiation promoting agent of the present invention contains one or more kinds of plant extracts selected from Satsuma mandarin, Onidokoro, Oyster, Kay, Peonies, Senkyu, Tsurudokudami, Biwa, and Matabi.

  In the present invention, after various parts of these plants are left undried or dried, an extract obtained by extraction with a solvent after crushing or pulverization, or by evaporating or lyophilizing the extraction solvent from the extract The resulting non-volatile content can be used.

  Examples of the extraction solvent include water, alcohols (eg, methanol, ethanol, ethylene glycol, propylene glycol, 1,3-butylene glycol, etc.), ketones such as acetone, ethers such as diethyl ether, and aromatics such as toluene. The various solvents, such as these, are mentioned, and can be used individually or in combination of 2 or more types.

  Citrus unshiu used in the present invention is a plant of the citrus family and its fruit is widely used for food. In the present invention, the trunk, bark, leaves, branches, branches and leaves, flowers, fruits, pericarp, seeds, roots and root barks of Satsuma mandarin are used, but other plants belonging to the same genus can also be used. Most preferably, the powder obtained by drying the peel is extracted with a solvent and the solvent is distilled off.

  The term “Dioscorea tokoro” used in the present invention is a plant of the genus Dioscorea. In the present invention, the whole plant, leaves, stems, flowers, fruits, pericarps, seeds, roots, rhizomes, and root barks of Onidoro are used, but other plants belonging to the same genus can also be used. Most preferably, the powder obtained by drying the rhizome is extracted with a solvent and the solvent is distilled off.

  The oyster (Diospyros kaki) used in the present invention is a plant belonging to the family Oysteraceae, and its fruit is widely edible. In the present invention, oyster trunk, bark, leaves, branches, branches and leaves, flowers, fruits, pericarp, seeds, roots, root barks are used, but other plants belonging to the same genus can also be used. Most preferably, the powder obtained by drying the leaves is extracted with a solvent and the solvent is distilled off.

  Cinnamomum cassia used in the present invention is a plant belonging to the family Aceraceae. In the present invention, the trunk, trunk bark, leaves, branches, branches and leaves, flowers, fruits, pericarps, seeds, roots, root barks of the silk are used, but other genus plants can also be used. Most preferably, the powder obtained by drying the trunk skin is extracted with a solvent and the solvent is distilled off.

  The peony (Paeonia lactiflora) used in the present invention is a plant of the Button family. In the present invention, peony whole plants, leaves, stems, flowers, fruits, pericarps, seeds, roots, root barks are used, but other plants belonging to the same genus can be used. Most preferably, it is obtained by extracting powder with dried roots with a solvent and distilling off the solvent.

  The cucumber (Cnidium officinale) used in the present invention is a plant of the Apiaceae family. In the present invention, whole leaves, leaves, stems, flowers, fruits, pericarps, seeds, roots, rhizomes and root barks of senkyu are used, but other genus plants can also be used. Most preferably, the powder obtained by drying the rhizome is extracted with a solvent and the solvent is distilled off.

Polygonum multiflorum used in the present invention is a plant belonging to the family Taceae.
In the present invention, the whole plant, leaves, stems, flowers, fruits, pericarps, seeds, roots, tuberous roots, and root barks of tsuldukudami are used, but other plants belonging to the same genus can also be used. Most preferably, the powder obtained by drying the tuberous root is extracted with a solvent and the solvent is distilled off.

  The loquat (Eriobotrya japonica) used in the present invention is a plant of the Rosaceae family. In the present invention, loquat trunk, stem bark, leaves, branches, branches and leaves, flowers, fruits, pericarps, seeds, roots, root barks are used, but plants belonging to the same genus can also be used. Most preferably, the powder obtained by drying the leaves is extracted with a solvent and the solvent is distilled off.

  The matabi (Actinidia polygama) used in the present invention is a plant of the family Tabata. In the present invention, the trunk, bark, leaves, branches, branches and leaves, flowers, fruits, worms, pericarps, seeds, roots and root barks of matabha are used, but plants belonging to the same genus can also be used. Most preferably, the powder obtained by drying the aneurysm is extracted with a solvent and the solvent is distilled off.

  The plant used in the present invention is one that is generally used as a component of medicine or folk medicine, food, and cosmetics, and its safety has been confirmed.

  The preadipocyte differentiation promoter of the present invention contains the herbal medicines alone or as a mixture. Although the total blending amount of the plant component varies depending on the dosage form, the evaporation residue may be used as it is, and the blending amount may be appropriately adjusted depending on the intended use. There is no restriction | limiting in particular in the usage-amount of the preadipocyte differentiation promoter of this invention, It can adjust suitably with a use or application.

  The preadipocyte differentiation promoting agent of the present invention can be administered orally or absorbed through the skin. Therefore, it can be formulated into various skin external preparations and bath preparations including foods and drinks, internal preparations and cosmetics. Moreover, it can also mix | blend with other food-drinks, a pharmaceutical, cosmetics, a bath agent, etc.

  In the case of a food or drink, it can be blended alone or in other food or drink. The food and drink may be in any form, and may be a solid such as biscuits, cookies, and candy, a liquid such as soft drinks, or a syrup. It is appropriate that the daily intake per adult is 0.01 to 5 g, but it can be changed as appropriate.

  When used as an internal preparation, it can be formulated as tablets, capsules, granules, powders, syrups and the like. In formulating, it can be formulated with a carrier such as an excipient, a lubricant, a binder, a disintegrant, and a coating agent. If necessary, coloring agents, fragrances, preservatives, and fragrances can be added. The amount per day for internal use is suitably 0.01 to 5 g in terms of the amount of extract for adults, but can be changed as appropriate.

  In the case of an external preparation, any dosage form such as paste, cream, gel, ointment, lotion, milky lotion, pack, powder, poultice may be used. In that case, all known bases, excipients, and carriers can be used. In that case, the content of the extract is suitably 0.001 to 50% by mass. The content of the extract when used as a bath agent is suitably 0.01 to 50% by mass.

  Furthermore, it can be used as an additive for livestock feed. In this case, an appropriate amount is added to the conventional animal feed.

Hereinafter, examples and test examples relating to herbal extracts will be described in order to specifically describe the present invention, but the present invention is not limited to these examples.

Example 1
50 g of powder obtained by drying the peel of Citrus unshiu was extracted using 50% by mass ethanol water as a solvent, and the solvent was distilled off to obtain 14.6 g.

Example 2
50 g of a powder obtained by drying Rhizome of Onidokoroko was extracted using 50% by mass ethanol water as a solvent, and the solvent was distilled off to obtain 5.1 g.

Example 3
50 g of powder obtained by drying oyster leaves was extracted using 50% by mass ethanol water as a solvent, and the solvent was distilled off to obtain 6.5 g.

Example 4
50 g of powder obtained by drying the trunk of the silica was extracted using 50% by mass ethanol water as a solvent, and the solvent was distilled off to obtain 5.0 g.

Example 5
50 g of ethanol powder was extracted from 50 g of dried peony roots, and the solvent was distilled off to obtain 17.9 g.

Example 6
50 g of powder obtained by drying the rootstock of senkyu was extracted using 50% by mass ethanol water as a solvent, and the solvent was distilled off to obtain 13.0 g.

Example 7
50 g of powder obtained by drying the roots of tsurudukudami was extracted using 50% by mass ethanol water as a solvent, and the solvent was distilled off to obtain 14.6 g.

Example 8
50 g of dry powder of loquat leaves was extracted using 50% by mass ethanol water as a solvent, and the solvent was distilled off to obtain 7.7 g.

Example 9
50 g of a powder obtained by drying the scab of Matatabi was extracted using 50% by mass ethanol water as a solvent, and the solvent was distilled off to obtain 6.8 g.

Example 10
0.01 g of each of the extracts obtained in Examples 1 to 9 was dissolved in hot water at 80 ° C., added to 25 g of jelly (house food) to make 100 g as a whole, and solidified in a refrigerator to obtain 9 types of jelly.

Example 11
Liquid paraffin was added to 0.1 g of each of the extracts obtained in Examples 1 to 9, 20 g of polyethylene glycol, 5 g of squalane, 3 g of apricot oil, 2 g of POE (12) sorbitan monooleate, and 1 g of POE (10) hydrogenated castor oil. The total amount was 100 g, and 9 types of bath preparations were obtained.

Example 12
Test using preadipocytes
(1) Cultivation of preadipocytes and addition of test substances Using mouse preadipocyte cell line (3T3-L1) distributed by Research Resource Bank, Human Science Foundation, subculture and differentiate based on the prescribed preparation method It was.

  Cells that had been passaged by a prescribed method were trypsinized to be detached from the petri dish to prepare a cell suspension. Cells were seeded in 24-well plates. The medium was changed every 2 days, and after the cells became confluent, the medium was further cultured for 2 days.

  Except for the medium, 980 μL of medium supplemented with a differentiation inducer (containing insulin, dexamethasone, and isobutylmethylxanthine) was added to each well. 20 μL of plant extract (dried product) dissolved in water-EtOH (1: 1) at a concentration of 25 and 5 mg / mL was added to each well. The final concentration of the plant extract is 0.5 and 0.1 mg / mL. Thereafter, it was placed in an incubator and cultured for 2 days. The medium was removed, and 980 μL of insulin-containing medium was added to each well. Next, 20 μL of a plant extract (dried product) dissolved in water-EtOH (1: 1) at a concentration of 25 and 5 mg / mL was added to each well and cultured in an incubator for 2 days.

  The medium was removed, 980 μL of medium was added to each well, and 20 μL of a plant extract (dried product) dissolved in water-EtOH (1: 1) at a concentration of 25 and 5 mg / mL was added to each well, and in an incubator. Cultured for 2 days.

(2) Oil red O staining test The 96-well plate after removal of the culture medium was washed with PBS (−), fixed with formalin, and then fat particles accumulated in the cells were stained with oil red O. . After oil red staining, the pigment was extracted with isopropyl alcohol, the fat was quantified, and the differentiation promoting action was evaluated.

(3) GPDH activity measurement After removing the test medium, it was washed with PBS (−), and a cell lysate was prepared with an enzyme extract and an ultrasonic crusher. A supernatant was obtained from this disrupted solution by centrifugation. Differentiation promoting action was evaluated using a glycerol-3-phosphate dehydrogenase (GPDH) activity measurement kit (Wako Pure Chemical Industries). An increase in GPDH activity serves as an indicator of differentiation promoting action.

  As shown in Table 1, the plant extracts of Examples 1 to 9 increase fat stained with oil red O in the process of differentiation of preadipocytes, and further increase GPDH activity, which plays an important role in differentiation Therefore, it is clear that it has an effect of promoting differentiation of preadipocytes.

Example 13
Animal testing
(1) Transdermal administration test using hairless mice While giving a general breeding feed to hairless mice (HR-1 mice), the extract obtained in Examples 1 to 9 was transdermally fed to the trunk in an amount of 1 mg / kg. Administered. This was performed for 20 days, and each group was opened and the amount of body fat was visually observed.

  As shown in Table 2, it is clear that the plant extracts of Examples 1 to 9 increase the body accumulated fat amount by transdermal administration to hairless mice and have a fat accumulation effect.

(2) Oral administration test using ICR mice The extract obtained in Examples 1 to 9 was orally administered in an amount of 50 mg / kg while giving general breeding feed to ICR mice. This was performed for 20 days, and each group was opened and the amount of body fat was visually observed.

  As shown in Table 3, it is clear that the plant extracts of Examples 1 to 9 increase the body accumulated fat amount by oral administration to ICR mice and have a fat accumulating effect.

Claims (6)

  A preadipocyte differentiation promoter comprising one or more plant extracts selected from Satsuma mandarin, Onidokoro, Oyster, Kay, Peonies, Senkyu, Tsurudokudami, Biwa and Matabi.   The fat accumulation agent containing the fat accumulation preadipocyte differentiation promoter of Claim 1.   A breast augmenter containing the fat accumulation preadipocyte differentiation promoting agent according to claim 1.   A fat accumulation and / or breast augmentation skin external preparation containing the fat accumulation preadipocyte differentiation promoting agent according to claim 1.   A fat accumulation and / or breast augmentation bath containing the fat accumulation preadipocyte differentiation promoting agent according to claim 1.   A fat-accumulating and / or breast-enhancing food or drink containing the fat-accumulating preadipocyte differentiation promoting agent according to claim 1.