KR101685581B1

KR101685581B1 - Compositions for improving skin wrinkle or moisture comprising Orostachys japonicus and composition prepared therefrom

Info

Publication number: KR101685581B1
Application number: KR1020150113928A
Authority: KR
Inventors: 김수경; 서동구; 차영주
Original assignee: 김수경; 서동구; 차영주
Priority date: 2015-08-12
Filing date: 2015-08-12
Publication date: 2016-12-12

Abstract

The present invention relates to a method for preparing a cosmetic composition for skin wrinkle reduction or skin moisturization using nature-derived materials including Orostachys japonicus and a cosmetic composition prepared thereby, wherein the method comprises the steps of: (A) mixing: Orostachys japonicus; at least one selected from the group consisting of green tea leaves, Cudranic triloba fruits, Huttuynia cordata, Saururus chinensis, Schisandra chinensis, Tremella fuciformis, and ginseng; brown rice; nuruk; grain syrup; useful microorganisms; and mineral concentrated water, adding deep sea water and loess water thereto, followed by first fermentation; (B) injecting air at the same temperature as in the first fermentation, followed by second fermentation; (C) mixing: Orostachys japonicus; Artemisia Herb; at least one selected from the group consisting of natsudaidai peal, deer antlers, wilfordi root, rhubarb root, Codonopsis, Secale cereal, oats, rice bran, black soybeans, apricot seeds, Cornus officinalis, Acorus gramineus, milkwort, Zizyphus spinosi, Bambusae Caulis, cassia seed, and liquorice; Ecklonia cava; birch sap; mineral concentrated water; and deep ocean water, followed by hot-water extraction; (D) filtering a fermentation material fermented in step (B) to separate a fermentation liquid and residues; (E) hot-water extracting the residues separated in step (D); and (F) mixing the fermentation liquid separated in step (D), the extract extracted in step (D), and the extract extracted in step (C), so the cosmetic composition has excellent effects of skin wrinkle reduction and moisturization.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for skin wrinkle improvement or skin moisturization using a naturally-occurring material including a natural wax,

The present invention relates to a method for producing a cosmetic composition for improving skin wrinkles or skin moisturizing using a naturally-occurring material containing wax, which is excellent in improving skin wrinkles and moisturizing effect, and a cosmetic composition prepared thereby.

Synaptic vesicles containing neurotransmitters at the time of neurotransmitter release must be fused with the presynaptic membrane to form a channel between the two interfaces. At this time, the protein that provides the fundamental force of membrane fusion is a protein complex called SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor). Membrane fusion between synaptic vesicles and synaptic membrane opens the neurotransmitter exit pathway, which involves the t-SNARE complex attached to the target membrane and v-SNARE attached to the vehicle. t-SNARE is a complex of two proteins called synaptaxin 1a and SNAP-25. These SNARE proteins are twisted like twine.

Upon such membrane fusion, rearrangement of the lipid bilayer, which is well known in the art, occurs. Since the biomembranes are pushing strongly against each other, these membranes are not fused spontaneously but strong forces are exerted from the outside to overcome the repulsive force between the membranes. At this time, the SNARE protein produces a strong force enough to overcome the repulsive force between the membranes. That is, the formation of the SNARE complex overcomes the interlaminar repulsion

And is a key phenomenon of exocytosis, including the release of neurotransmitters.

To control muscle relaxation and contraction, there is a neuromuscular junction in the upper layer of the muscle, and a nerve terminal is loaded with synaptic vesicles. BoNT (Botulinum neurotoxin, hereinafter referred to as 'Botox') is a protease that cleaves the SNARE protein, a key protein involved in neurotransmitter release. Botox cleaves the SNARE protein and blocks neurotransmission, resulting in paralysis of the muscle cells that have infiltrated Botox. While Botox blocks the transmission of nerves by irreversibly cleaving the SNARE protein, the so-called "Botox Bottle" is a sort of "competitive inhibitor" that interferes with neurotransmission by inhibiting the formation of the SNARE complex. Argyrenelin, a major component of currently available Botox products, has an uncertain effect and is disadvantageous in price due to the nature of synthetic peptides and is not consumer friendly.

Development of inhibition of neurotransmission by using natural extracts can provide much less expensive raw materials than peptides and it is a natural material so it can be approached to consumers in the end product development easily. do.

Skin wrinkles are an apparent feature of the most prominent skin aging caused by internal factors such as age and stress, or by external environmental factors such as air pollution or ultraviolet radiation. The wrinkles occur on the face, neck, neck, elbow, armpits, hands and feet of the body such as the forehead, the eyes, the eyes and the mouth. Most of them begin to become noticeable before and after 30 years of age, and their number, depth and range increase with age.

The major cause of wrinkles is the formation of crosslinks in collagen fibers and elastic fibers, which ultimately occupy the majority of skin dermis components, and changes in the production and degradation of these fibers. can do. Antioxidants such as vitamin E, beta-carotene, vitamin C and glutathione, radical scavengers and substances promoting dermal collagen synthesis such as vitamin A, which are used to control skin aging, Has been used as a material.

However, most of these cosmetic active ingredients are strongly irritated by the skin or are unstable in the formulations themselves, resulting in discoloration, detachment and sedimentation, and the inherent activity of the raw material is reduced.

On the other hand, recently, researches have been made that collagen cleavage degraded by ultraviolet light modifies intracellular signal transduction pathways, thereby inhibiting the synthesis of hyaluronic acid present in the skin, thereby reducing skin moisturizing power.

The hyaluronic acid is known to have a high moisture-retaining ability due to its easy binding with moisture. Particularly, it is known that at least 50% of the hyaluronic acid in the skin exists in the skin, and the skin bulge, the diffusion of metabolites and nutrients, Strengthen. Conversely, the amount of hyaluronic acid in the dermis of the skin decreases with aging, and when the anti-aging substance such as retinoic acid is treated, loss of hyaluronic acid is prevented and skin moisturizing ability is restored .

Therefore, ultraviolet irradiation causes loss of collagen, which not only causes wrinkles of the skin but also deteriorates the skin moisturizing function.

Recently, various cosmetic products using natural products have been developed to reduce skin irritation caused by various chemical substances and the like. These natural materials are not only less harmful to the skin, but also become more appreciated as a raw material for cosmetics as consumers' response increases.

Korea Patent No. 2014-0118172 Korean Registered Patent No. 2011-0042851 Korean Registered Patent No. 2008-0020030

It is an object of the present invention to provide a method for manufacturing a cosmetic composition for improving skin wrinkles or skin moisturizing using a natural-derived material including worts, which is excellent in improvement of skin wrinkles and moisturizing effect.

Another object of the present invention is to provide a cosmetic composition prepared by the above-mentioned method.

In order to accomplish the above object, the present invention provides a cosmetic composition for improving skin wrinkles or skin moisturizing using a natural-derived material comprising a feed, comprising (A) The first fermentation is carried out by mixing the microorganism, mineral water, mineral water, mushroom, ginseng, brown rice, nuruk, orchid, EM solution, mineral water and deep sea water and yellow loam;

(B) injecting air under the same temperature as that of the primary fermentation to perform secondary fermentation;

(C) Cereal, lemon, Cheongpiae, antler, white mulberry, rhubarb, savannah, rye, oats, rice, black beans, apricot seeds, Condensing mineral water and deep sea water to extract hot water;

(D) separating the fermented product fermented in step (B) into a fermentation broth and a residue;

(E) hot water extraction of the residue separated in step (D); And

(F) mixing the fermentation broth separated in step (D), the extract extracted in step (E), and the extract extracted in step (C).

50 to 600 parts by weight of green tea leaves, guacamole fruit, obtusa, Sambuchecho, Omija, silver mushroom and ginseng are added to 100 parts by weight of the green tea in step (A) 5 to 10 parts by weight of brown rice; 5 to 10 parts by weight of yeast; 100 to 300 parts by weight of crude oil; 100 to 300 parts by weight of useful microorganisms; 20 to 100 parts by weight of mineral concentrated water; 1500 to 2000 parts by weight of deep ocean water; And 1,500 to 2,000 parts by weight of yellow clay.

In the step (A), the primary fermentation may be performed at 20 to 50 ° C for 12 to 20 days.

In the step (B), the secondary fermentation may be performed for 12 to 20 days.

100 to 250 parts by weight of a lash to 100 parts by weight of the feed in the step (C); 100 to 2000 parts by weight of a group consisting of green tea, green tea, green tea, white sage, rhubarb, savannah, rye, oats, rice bran, black beans, apricot seeds, corn oil, 30 to 100 parts by weight of a menthol; 300-1000 parts by weight of birch sap; 20 to 50 parts by weight of mineral concentrated water; And deep sea water at 1000 to 20,000 parts by weight.

In the step (C), the hot-water extract may be a mixture obtained by extracting the mixture at 70 to 90 ° C. for 100 to 130 hours.

In the step (E), the hot-water extract may be a mixture of the residue and water at a weight ratio of 1: 1 to 3 and extracted at 70 to 90 ° C for 10 to 30 hours.

In step (F), the fermentation broth separated in step (D), the extract extracted in step (E), and the extract extracted in step (C) are mixed at a weight ratio of 1: 0.2-0.8: 0.5-3.0 .

According to another aspect of the present invention, there is provided a cosmetic composition for improving skin wrinkles or skin moisturizing, comprising the composition of the present invention.

The composition may be in the form of a solution, a topical ointment, a cream, a foam, a nutritional lotion, a softening water, a soft water, an emulsion, a makeup base, an essence, a soap, a liquid cleansing liquid, , A powder, a soap, a surfactant-containing cleansing, an oil, a powdered foundation, an emulsion foundation and a wax foundation.

The cosmetic composition for improving skin wrinkles or skin moisturizing according to the present invention inhibits the release of neurotransmitters in PC12 cells and can have an effect of improving skin wrinkles as if botox were administered.

In addition, the cosmetic composition of the present invention is excellent in biosynthesis ability of hyaluronic acid and exhibits excellent skin moisturizing ability. It is preferable that the cosmetic composition is applied to cosmetics of a formulation such as cream for application.

The present invention relates to a method for producing a cosmetic composition for improving skin wrinkles or skin moisturizing using a natural-derived material containing wax, which is excellent in improving skin wrinkles and moisturizing effect, and a cosmetic composition prepared thereby.

In the present invention, "skin wrinkle" refers to a scarring caused by skin damage, which may be caused by a cause of a gene, a decrease in collagen and elastin present in the skin dermis, and an external environment.

In the present invention, "improvement of skin wrinkles" refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating already formed wrinkles.

Hereinafter, the present invention will be described in detail.

A cosmetic composition for improving skin wrinkles or skin moisturizing using a natural-derived material comprising the feed of the present invention comprises: (A) (B) injecting air under the same temperature as that of the primary fermentation to perform secondary fermentation; (C) heating and hydrothermal mixing with other natural materials; (D) separating the fermented product fermented in step (B) into a fermentation broth and a residue; (E) hot water extraction of the residue separated in step (D); And (F) mixing the fermentation broth separated in step (D), the extract extracted in step (E), and the extract extracted in step (C).

In the step (A), first, Green tea leaf, Gucci mung bean, Persimmon, Saururus chinensis, Omija, silver mushroom and ginseng; Brown rice; yeast; Cho Chung; Useful microorganisms; After mixing the mineral concentrated water, deep ocean water; And yellow clinging factor are added to the first fermentation.

Orostachys japonicus A. Berger is a perennial herbaceous plant with a crassacea called Iwamatsu and Okso (松松). It is used for the fever, swelling, hemostasis, It is widely used for cancer treatment by therapy. It is a fleshy perennial herb that grows on the side of a rock, but has a characteristic of blossoming and fruiting when it blooms.

When at least one selected from the group consisting of green tea leaf, Gucci mongolian, obtusa, Saururus chinensis, Omija, silver mushroom and ginseng is fermented together with persimmon, it improves the biosynthesis performance of hyaluronic acid, Wrinkles can be improved. Preferably, the green tea leaf, Gucci mung bean, Sophora japonica, Saururus chinensis, Omija, Silver mushroom, and Ginseng are all used.

The content of at least one selected from the group consisting of green tea leaf, Gucci mulberry fruit, obtusa, Sambucoptera, Omija, silver mushroom and ginseng is used in an amount of 50 to 600 parts by weight based on 100 parts by weight of the green tea powder. 1: 1: 1: 1: 1: 1: 1. When the content of at least one selected from the group consisting of green tea leaves, guacamole fruit, obtusa, Saururus chinensis, Omija, silver mushroom and ginseng is lower than the lower limit value, wrinkles are not improved and the biosynthesis performance of hyaluronic acid is not improved. , Skin trouble may occur.

The brown rice is preferably brown rice, and it is mixed with the above-mentioned wort to improve the wrinkles of the skin during fermentation and improve the moisturizing power. The brown rice is mixed with 5 to 10 parts by weight, preferably 6 to 8 parts by weight, based on 100 parts by weight of the raw cotton. When the content of the brown rice is less than the lower limit, excellent wrinkles are not improved and the biosynthesis performance of the hyaluronic acid is not improved. In the case of exceeding the upper limit value, further excellent effects can not be obtained and resources are wasted.

The yeast is a traditional yeast fermentation product of Korea and commonly used yeast is mixed with useful microorganisms to ferment other naturally occurring substances to improve skin wrinkles and improve moisturizing power. If any of the above-mentioned yeast and useful microorganisms is not used, skin wrinkles are not promoted and the biosynthesis performance of hyaluronic acid is not improved.

The koji is mixed in an amount of 5 to 10 parts by weight, preferably 6 to 8 parts by weight based on 100 parts by weight of the wort. If the content of the yeast is below the lower limit, the fermentation may not be performed. If the content of the yeast exceeds the upper limit, the yeast is used more than necessary and resources are wasted.

The above-mentioned starch is used for fermentation, and specific examples thereof include cornstarch, bellflower, cornstarch and black garlic, preferably cornstarch.

The content of tonic acid is 100 to 300 parts by weight, preferably 150 to 200 parts by weight, based on 100 parts by weight of the feed. Fermentation may not be carried out when the content of oranges is less than the above lower limit, and in the case of exceeding the upper limit, waste more than necessary is used and resources are wasted.

The beneficial microorganism (EM) is mixed with the yeast to ferment naturally occurring substances, thereby improving skin wrinkles and improving moisturizing power.

The content of the useful microorganism is 100 to 300 parts by weight, preferably 100 to 150 parts by weight based on 100 parts by weight of the feed. When the content of the useful microorganisms is less than the lower limit, the skin wrinkles are not improved and there is no moisturizing power. If the content is above the upper limit, biosynthesis ability of hyaluronic acid is lowered.

The mineral-enriched water is obtained by concentrating deep-sea water having a hardness of 300 mg / L to a hardness of 230,000 to 290,000 mg / L. The hardness is an index indicating the content of calcium carbonate in the water, which corresponds to the sum of the contents of calcium ions and magnesium ions in water. The higher the hardness, the larger the amount of minerals contained. When the hardness of the mineral-concentrated water is less than the lower limit, minerals such as calcium and magnesium can not be supplied to the skin. If the hardness of the mineral-concentrated water is above the upper limit, skin troubles may occur.

The content of the mineral-concentrated water is 20 to 100 parts by weight, preferably 30 to 50 parts by weight based on 100 parts by weight of the feed. When the content of the mineral-concentrated water is lower than the lower limit, skin wrinkle improvement and moisturizing power enhancement can not be expected, and skin trouble may occur when the content is above the upper limit.

In the present invention, it is necessary to first mix the mineral-concentrated water with high hardness and the naturally-derived substances to apply or infiltrate a large amount of concentrated minerals on the surface of the naturally-occurring substances, and then ferment under deep sea water and yellow lozenges. Minerals can improve their efficacy by fermentation. At this time, when the deep sea water and the yellow clay are added together with mineral concentrated water without adding them later, the wrinkles of the skin can not be improved and the biosynthesis ability of hyaluronic acid may be lowered.

The content of the deep sea water is 1500 to 2000 parts by weight, preferably 1600 to 1700 parts by weight, based on 100 parts by weight of water. If the content of the deep sea water is out of the above range, it may interfere with fermentation.

The above-mentioned yellow clay is a supernatant after standing for 20 to 25 hours in a mixture of yellow soil and water, and contains various minerals such as potassium, sodium and iron, which are contained in mineral concentrated water and deep seawater, .

The content of the yellow clay particles is 1500 to 2000 parts by weight, preferably 1600 to 1700 parts by weight, based on 100 parts by weight of the green tea. When the content of the yellow clay is less than the lower limit, various minerals can not be supplied to the skin. If the content is above the upper limit, the yellow clay is used more than necessary and resources are wasted.

The mixture mixed in the step (A) is firstly fermented at 20 to 50 ° C, preferably 25 to 30 ° C for 12 to 20 days, preferably 13 to 14 days. During the first fermentation, the fermentation vessel is turned over once a day to allow the fermentation to be performed well. When the fermentation temperature and time are out of the above range, fermentation may not be performed well, and in particular, if the fermentation temperature is higher than the upper limit value, skin trouble may be caused.

Next, in the step (B), air is injected under the same temperature as that of the primary fermentation for secondary fermentation.

Air is injected for 12 to 20 days, preferably 13 to 14 days at the same temperature as the primary fermentation, thereby performing secondary fermentation while air bubbles are formed in the fermentation product.

When fermentation is carried out without air injection, secondary fermentation is not smoothly performed and, in some cases, toxicity may be induced.

In the present invention, step (C) is performed separately from steps (A) and (B).

Meanwhile, in the step (C), Lobe; At least one selected from the group consisting of sweet potato, green peach, antler, white sage, rhubarb, savannah, rye, oat, rice bran, black bean, apricot seeds, corn oil, Gut; Birch sap; Mineral concentrated water; And deep sea water are mixed and subjected to hot water extraction.

In combination with the fermented product prepared in the step (B), lashes are used to significantly enhance the biodegradability of skin wrinkles and hyaluronic acid.

The content of the leaf blade is 100 to 250 parts by weight, preferably 120 to 150 parts by weight, relative to 100 parts by weight of the feed. When the content of the leaf is less than the lower limit, the skin wrinkles are not improved and the moisture resistance is not improved. When the content is higher than the upper limit, wrinkles of the skin may occur and the moisturizing power may be lowered.

In addition, in order to further inhibit the membrane fusion phenomenon and inhibit the release of neurotransmitters by mixing with the fermented product prepared in the step (B), it is preferable to use a chewing gum, antler, white liquor, rhubarb, One or more selected from the group consisting of rice gruel, black bean, apricot seed, corn oil, seokjangpo, raw paper, sanjiton, killing, killer and licorice are preferably used together.

The content of at least one selected from the group consisting of green tea, antler, white mulberry, rhubarb, savannah, rye, oats, rice bran, black beans, apricot seeds, corn oil, Based on 100 parts by weight of the resin. If the content is below the lower limit, the biosynthesis performance of hyaluronic acid may not be improved. If the content is above the upper limit, skin troubles and toxicity may be caused.

Also, the fermented product prepared in step (B) is mixed with the fermented product to improve the wrinkles and improve the moisturizing power.

The content of chewing gum is 30 to 100 parts by weight based on 100 parts by weight of wafers. When the amount of menthol content is less than the lower limit, wrinkles can not be improved and the moisturizing ability can not be improved, and when the content is above the upper limit value, the moisturizing ability may be remarkably lowered.

In addition, the birch sap is mixed with naturally-derived materials to further improve the biosynthesis performance of hyaluronic acid, thereby greatly improving the moisturizing power. The content of birch sap is 300 to 1000 parts by weight per 100 parts by weight . When the content of the birch sap is less than the lower limit, moisture resistance may not be improved, and when the content is above the upper limit, wrinkle improvement may be lowered.

The content of the mineral-enriched water is 20 to 50 parts by weight, preferably 25 to 30 parts by weight based on 100 parts by weight of water. When the content of the mineral-concentrated water is less than the lower limit, the skin wrinkles are not improved and the moisturizing power is not improved.

The deep sea water is used for hot water extraction and is preferably used in an amount of 1000 to 20,000 parts by weight based on 100 parts by weight of the feed.

The hot-water extract in step (C) is obtained by extracting the mixture at 70 to 90 ° C. for 100 to 130 hours, preferably 120 to 130 hours. If the extraction temperature and time are out of the above range, the active substances may not be extracted.

Next, in step (D), the secondary fermentation product fermented in the step (B) is filtered and separated into a fermentation broth and a residue.

The secondary fermented product is separated by filtration into a fermentation broth and a residue.

Next, in the step (E), the residue separated in the step (D) is extracted with hot water.

The residue separated in the step (D) and water are mixed at a weight ratio of 1: 1 to 3 and extracted with hot water at 70 to 90 ° C for 10 to 30 hours, preferably 15 to 20 hours to obtain an extract.

When the extraction temperature and the extraction time are out of the above range, the effective substance contained in the residue can not be extracted.

Next, in step (F), the fermentation broth separated in step (D), the residue extract extracted in step (E), and the extract extracted in step (C) are mixed.

The fermentation broth separated in step (D), the residue extract extracted in step (E), and the extract extracted in step (C) are mixed at a weight ratio of 1: 0.2-0.8: 0.5-3.0, 0.7: 1.0-1.5. When the content of the residue extract extracted at step (E) and the extract extracted at step (C) are lower than the lower limit value on the basis of the fermentation broth separated in step (D), skin wrinkles and moisturizing power can not be enhanced, If it is toxic, it can cause skin trouble.

If any of the fermentation broth separated in step (D), the residue extract extracted in step (E), and the extract extracted in step (C) is omitted, the skin wrinkles and skin moisturizing ability can not be improved.

The naturally occurring materials used in the present invention should be manufactured according to the manufacturing method of the present invention to exert mutually complementary pharmacological effects, thereby inhibiting the membrane fusion phenomenon and inhibiting the release of neurotransmitters to improve skin wrinkles, Improves skin's moisture retention by improving performance.

The cosmetic composition for skin wrinkle improvement or skin moisturizing of the present invention may be blended with other cosmetic ingredients usually added to the cosmetic composition, if necessary, in addition to the cosmetic composition for skin wrinkle improvement or skin moisturizing, which is prepared according to the above- As the component, there may be mentioned a preservative component, a moisturizer, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorbent, an antiseptic, a bactericide, an antioxidant, a pH adjuster, , Purified water, and the like.

The cosmetic composition for improving skin wrinkles or moisturizing the skin may be prepared in the form of an emulsified formulation and a solubilized formulation commonly used in the art, and examples thereof include a solution, an external ointment, a cream, a foam, a nutritional lotion, Lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations and emulsions, emulsions, lotions, makeup bases, essences, soaps, liquid cleansing agents, sunscreen creams, sun oils, suspensions, emulsions, pastes, Wax foundation, and the like. In particular, the cosmetic composition for improving skin wrinkles or skin moisturizing of the present invention is preferably applied to a cream.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.

Preparation Example 1. Preparation of the compound of step (A)

83 parts by weight of green tea leaves, 83 parts by weight of green tea leaves, 83 parts by weight of Gucci mulberry fruit, 83 parts by weight of Seaweed, 83 parts by weight of Saururus chinensis, 83 parts by weight of Omija, 83 parts by weight of silver mushroom, 83 parts by weight of ginseng, 6.6 parts by weight of cornstarch and 166 parts by weight of cornstarch were mixed with 33 parts by weight of mineral-concentrated water and 100 parts by weight of useful microorganisms. The mixture was mixed with 1600 parts by weight of deep sea water and 1600 parts by weight of yellow clay.

Preparation Example 2. Preparation of the compound of step (C)

100 parts by weight of green tea, 100 parts by weight of green tea, 100 parts by weight of green tea, 100 parts by weight of green tea, 100 parts by weight of green tea, 100 parts by weight of green tea, 100 parts by weight of green tea, 100 parts by weight of black beans, 100 parts by weight of apricot seeds, 100 parts by weight of corn oil, 100 parts by weight of seaweed oil, 100 parts by weight of ground koji, 100 parts by weight of raw paper, 100 parts by weight of acidolin, 500 parts by weight of birch sap, 25 parts by weight of mineral concentrated water and 10000 parts by weight of deep sea water were added and mixed, followed by extraction at 80 to 120 hours to obtain a hot-water extract.

Example 1.

The compound of Preparation Example 1 was subjected to primary fermentation (inversion of the fermentation vessel once a day) for 30 to 14 days, followed by air injection at the same temperature for 14 days to perform secondary fermentation (fermentation vessel flipping once every 2 days) The secondary fermentation product was filtered and separated into fermentation broth and residue.

The residue and water were mixed at a weight ratio of 1: 2 and then extracted at 80 to 20 hours to obtain a residue extract.

The filtered fermentation broth, the residue extract and the hot water extract of Preparation Example 2 were mixed at a weight ratio of 1: 0.7: 1.0 to prepare a cosmetic composition.

Comparative Example 1. Fermentation Omission

The compound of Preparation Example 1 and the hot-water extract of Preparation Example 2 were mixed at a weight ratio of 1: 1.7 to prepare a cosmetic composition.

Comparative Example 2. Omission of residue extract

The same procedure as in Example 1 was carried out except that the filtered fermentation broth without using the residue extract and the hot water extract of Preparation Example 2 were mixed at a weight ratio of 1: 1.7 to prepare a cosmetic composition.

Comparative Example 3: The hot-water extract of Production Example 2 was omitted

The same procedure as in Example 1 was carried out except that the filtered fermentation broth and the residue extract were mixed at a weight ratio of 1: 1.7 to prepare a cosmetic composition.

<Test Example>

Test Example 1. Cytotoxicity test

Cytotoxicity was measured according to the concentration of the cosmetic composition prepared in Examples and Comparative Examples.

Cytotoxicity was measured by the method of Rochem et al. In order to carry out the test in the safety concentration range of the sample. Cells (2 × 10 ⁵ cells / well) (RAW 264.7) were seeded in 24-well plates and cultured for 24 hours. After that, the medium was removed and 1 mL of the medium containing 3% of the serum in which the extract was dissolved was dispensed and cultivation was carried out for 24 hours. After 24 hours, 0.25 mL of XTT-PMS solution (1 mg XTT and 10 g PMS / mL of MEM without phenol red) was added to each well and incubated for another 2 hours. The degree of cytotoxicity was determined by measuring the absorbance of formazan at 450 nm using a microplate reader. The medium was MEM supplemented with 10% (v / v) fetal bovine serum (FBS), 0.5% (v / v) 50 g / mL streptomycin, 50 IU / mL penicillin, 0.125 g / mL fungizone, 3.024 g sodium bicarbonate And incubated at 37 ℃, 5% CO _2, 95% humidified air.

division Cell viability (%) 10 [mu] g / ml 25 [mu] g / ml 50 [mu] g / ml 100 [mu] g / ml Example 1 119.1 113.7 110.8 107.9 Comparative Example 1 58.1 54.6 50.0 41.3 Comparative Example 2 103.1 100.4 91.5 89.6 Comparative Example 3 101.1 97.2 90.5 87.7

As shown in Table 1, it was confirmed that the cosmetic composition prepared according to Example 1 of the present invention showed no toxicity in the interval of 10 to 100 占 퐂 / ml.

On the other hand, in Comparative Example 1, toxicity was observed in the whole interval of 10 to 100 占 퐂 / ml, whereas Comparative Examples 2 and 3 showed toxicity from 50 占 퐂 / ml and 25 占 퐂 / ml respectively.

Test Example 2. Membrane fusion Inhibitory effect

The effect of inhibiting membrane fusion on the cosmetic composition obtained in the above Examples and Comparative Examples was tested. SNARE proteins called SNAP25 (NMO11428), Trust ShinA1 (AF217197) and VAMP2 (NMO12663) were purified from E. coli Codon (+) RIL, Novagen transformed by biotechnology to produce SNARE proteins . First, palmitoyloleoyl phosphatidylcholine (POPC) (62 mol%), Dioleoyl phosphatidylserine (DOPS) (35 mol%) was added to make a liposome, ), 1-oleoyl-2- [N- (7-nitro-2,1,3-benzoxadiazol-4- yl) amino] caproyl phosphatidylserine

oleoyl-2- [N- (7-nitro-2,1,3-benzoxadiazol-4-yl) amino] caproyl phosphatidylserine; NBD-PS) (1.5 mol%) and Rhodamin-PE (1.5 mol%) which is a fluorescent substance were mixed to prepare a 10 Mm v-vesicle. To make a lyphosome not labeled with a fluorescent substance DOPS and POPC were mixed at a molar concentration of 35:65 to form a 50 Mm tribe lysate. The mixture was mixed at a molar concentration of 1: 1 and reacted at room temperature for 1 hour to prepare a complex composed of purified SNAP25 and authentic Shin-1a. The mixture was mixed so that the molar ratio of the liposome with the fluorescent substance was 100: 1, VAMP2 was conjugated to a liposome containing a fluorescent substance in a molar ratio of 50: 1. Then, the two types of liposomes were dialyzed using a 10 kDa dialysis membrane at 4 ° C for 24 hours with stirring, and then mixed at a weight ratio of 3: 7 (v-vesicle: t-vesicle) To the mixture containing trust Shin I and VAMP-2, methanol as a control and the compositions of the examples and comparative examples of the present invention were added. Subsequently, the fluorescence intensity was measured using a fluorescence intensity measuring instrument (Model: SpectraMax M2, manufacturer: Molecular Device).

The fluorescence intensity of the control group was set as a reference, and relative inhibition ratios (%) in the compositions of the examples and the comparative examples were calculated.

division Membrane fusion inhibition rate (%) 10 [mu] g / ml 25 [mu] g / ml 50 [mu] g / ml 100 [mu] g / ml Control group 100 100 100 100 Example 1 0 0 27 45 Comparative Example 1 94 95 95 98 Comparative Example 2 76 80 82 90 Comparative Example 3 54 69 75 81

As shown in Table 2 above, the cosmetic composition prepared according to Example 1 of the present invention has a low fluorescence intensity, which means that it inhibits the membrane fusion phenomenon. When calculated by the membrane fusion inhibition rate, it is 10 to 25 ㎍ / Lt; RTI ID = 0.0 > ml < / RTI > As described above, Example 1 effectively inhibits the shape of the membrane fusion between SNARE proteins, thereby exhibiting excellent skin wrinkle-reducing effects.

On the other hand, it was confirmed that Comparative Examples 1 to 3 did not have excellent membrane fusion inhibition rates.

Test Example 3. Inhibition of neurotransmitter release in PC12 cells

PC12 cells were cultured in collagen coated plates (60 mm dish) in Ham's F12K media containing 10% fetal calf serum and 5% fetal bovine serum and antibiotics. After submerging the culture medium, 2 ml of PBS was added to the culture medium, and the cells were separated from the dish wall by pipetting, and the cells were collected by centrifugation at 1,000 xg for 5 minutes. The cells were then pipetted to disperse the cell pellet , And the cells were placed in a new culture plate and cultured in an incubator in which 5% CO ₂ gas was supplied at 37 ° C. The [3H] - noradrenaline used in the experiments was purchased from Amersham. After the cells were inhaled from the plate of PC12 cells, PBS was added and the cells were detached from the plate wall. Cell numbers were measured with a hematometer and the cells were inoculated into a fresh medium at a concentration of 2 x ¹⁰ cells / ml. After 24 hours, the noradrenaline assay buffer ([3H] -NA, 1.5 μCi / ml) was added, followed by introduction in a carbon dioxide gas incubator for 90 minutes. After the reaction, the buffer solution was removed, and the mixture was washed three times with PBS. Then, the cosmetic composition of Examples and Comparative Examples of the present invention was added to a new medium and reacted for 30 minutes. Verapamil was used as a positive control and treated in the same manner as above. After the medium was removed, a high concentration of K + buffer was added. After incubation in a carbon dioxide gas incubator for 12 minutes, the supernatant was recovered to measure the release of [3H] -nonadrenaline by a scintillation counter. It was confirmed by the above method that the cosmetic composition inhibited the release of dopamine derivative noradrenaline in PC12 cells, and the ratio (%) of noradrenaline in each experimental group was calculated based on the high concentration of K + buffer.

division Nor adrenaline ratios (%) 10 [mu] g / ml 25 [mu] g / ml 50 [mu] g / ml 100 [mu] g / ml High concentration K ⁺ buffer solution 98 98 98 98 Verapamil 19 22 25 30 Example 1 21 20 26 32 Comparative Example 1 98 98 99 98 Comparative Example 2 95 96 98 98 Comparative Example 3 91 94 97 98

As shown in Table 3, it was confirmed that the cosmetic composition prepared according to Example 1 of the present invention had a high inhibitory effect on the release of neurotransmitters. Therefore, the cosmetic composition of Example 1 can exhibit excellent skin wrinkle-reducing effect by inhibiting the release of neurotransmitter.

On the other hand, Cited References 1 and 3 confirmed that the release inhibiting effect of the neurotransmitter was scarcely observed.

Test Example 4. Wrinkle improvement and Moisture power Clinical effect

A sample in which the present invention (Example 1, Comparative Example 1-3) was applied to 100 persons (25 persons per each sample) was applied to 1,3-butylene glycol at 10 占 퐂 / ml After that, it was applied twice a day in the morning and in the evening for 3 months. The degree of wrinkle improvement and moisturizing after use was judged according to the following criteria.

division No improvement Slight improvement A significant improvement Example 1 0 3 22 Comparative Example 1 25 0 0 Comparative Example 2 22 3 0 Comparative Example 3 20 5 0

As shown in Table 4 above, the cosmetic composition prepared according to Example 1 of the present invention exhibited excellent wrinkle-improving effect and skin moisturizing power was improved.

On the other hand, Comparative Example 1 showed no improvement in wrinkle and skin moisturizing ability, and Comparative Examples 2 and 3 also showed low wrinkle and skin moisturizing effect.

Test Example 5. Hyaluronic acid biosynthesis performance

Fibroblasts isolated from human skin were cultured in 24 wells at a density of 10 ⁶ per well and grown to about 90%. After culturing for 24 hours in a serum-free medium, the cosmetic composition (10 μg / ml) of the above Examples and Comparative Examples was dissolved in serum-free medium and treated at a concentration of 10 ^-4 mol per ml and cultured in a CO ₂ incubator for 24 hours.

division Untreated group Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Hyaluronic acid sum performance (%) 100 171 100 102 105

As shown in Table 5, it was confirmed that the cosmetic composition prepared according to Example 1 of the present invention had an excellent hyaluronic acid biosynthesis enhancing effect as compared with Comparative Examples 1 to 3.

On the other hand, in Comparative Examples 1 to 3, it was confirmed that the effect of hyaluronic acid biosynthesis was almost negligible.

Test Example 6. Skin Moisture power Measure

The skin moisturizing power of the cosmetic composition prepared according to the above Examples and Comparative Examples was measured for 20 subjects as follows. Skin moisture resistance was evaluated by measuring the static load capacity of the skin according to the moisture content using a corneometer (Corneometer CM820: Courage, Khazaka) as a measuring instrument.

Each subject was rinsed with water before the experiment and adapted to the external environment for 30 minutes. The experimental conditions were the same at room temperature 23 ~ 25 ℃ and relative humidity 38 ~ 42%. The sample (10 μg / ml) was applied to the inside of the upper arm (2 × 2 cm) and dried for 1 minute. Then, 20 μl of purified water was applied to each application site. After the water was wiped with the tissue, The conductance (%) was calculated according to the following formula (1) by measuring the electrostatic capacity of the skin surface of each applied region from the minute to the 10 minute using a coneometer.

[Equation 1]

Conductivity (%) = (average Cv value measured 5 times per minute - Cv value before application of sample / immediately after application (0 minute) measured value - Cv value before application of sample) X 100

division Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 0 minutes 100.00 100.00 100.00 100.00 1 minute 82.46 65.46 72.19 75.46 2 minutes 78.13 60.15 68.41 70.15 3 minutes 74.58 54.76 62.71 64.39 4 minutes 69.42 48.71 54.85 59.45 5 minutes 63.28 40.43 48.27 52.11 6 minutes 59.77 33.16 40.16 45.80 7 minutes 53.62 28.18 31.69 39.02 8 minutes 50.38 23.47 28.14 30.15 9 minutes 45.75 18.49 20.45 23.64 10 minutes 41.27 11.17 16.77 19.79

As shown in Table 6 above, it was confirmed that the cosmetic composition prepared according to Example 1 of the present invention exhibited excellent moisture resistance as compared with Comparative Examples 1 to 3.

On the other hand, in Comparative Examples 1 to 3, it was confirmed that the moisture resistance was extremely low.

<Formulation example>

Hereinafter, formulation examples of the composition containing the extract of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Formulation Example 1. Preparation of cosmetic product of emulsified formulation

Cosmetics of emulsified form such as nutrient lotion, cream, essence, and cosmetics of solubilized form such as softened longevity were prepared.

Emulsifier type cosmetics were prepared with the compositions shown in Table 7 below. The production method is as follows.

1) A mixture of raw materials 1 to 9 was heated to 65 to 70 占 폚.

2) The starting material of 10 was added to the mixture of step 1).

3) The mixture of raw materials 11 to 13 was completely dissolved by heating to 65 to 70 占 폚.

4) While the above step 3) was carried out, the mixture of step 2) was gradually added and emulsified at 8,000 rpm for 2 to 3 minutes.

5) The raw material of 14 was dissolved in a small amount of water and then added to the mixture of step 4) and further emulsified for 2 minutes.

6) The raw materials of 15 to 17 were each weighed, and then put into the mixture of step 5) and further emulsified for 30 seconds.

7) The mixture of step 6) was degassed after emulsification and cooled to 25-35 占 폚 to prepare an emulsifier-type cosmetic.

Furtherance Emulsifier type 1 Emulsifier type 2 Emulsifier type 3 One Stearic acid 0.3 0.3 0.3 2 Stearyl alcohol 0.2 0.2 0.2 3 Glyceryl monostearate 1.2 1.2 1.2 4 Wax 0.4 0.4 0.4 5 Polyoxyethylene sorbitan
Monolauric acid ester 2.2 2.2 2.2 6 Methyl paraoxybenzoate 0.1 0.1 0.1 7 P-hydroxybenzoic acid profile 0.05 0.05 0.05 8 Cetyl ethyl hexanoate 5 5 5 9 Triglyceride 2 2 2 10 Cyclomethicone 3 3 3 11 Distilled water ~ 100 ~ 100 ~ 100 12 Concentrated glycerin 5 5 5 13 Triethanolamine 0.15 0.15 0.15 14 Polyacrylic acid polymer 0.12 0.12 0.12 15 Pigment 0.0001 0.0001 0.0001 16 incense 0.10 0.10 0.10 17 The cosmetic composition of Example 1 0.0001 One 10

Formulation example 2. Solubilization Manufacture of Cosmetics for Formulation

Cosmetics of solubilized formulations were prepared with the compositions shown in Table 8 below. The production method is as follows.

1) 2 to 6 raw materials were put into 1 raw material (purified water) and dissolved using a mixer.

2) Raw materials 8 to 11 were completely dissolved in 7 raw materials (alcohol).

3) The mixture of step 2) was slowly solubilized by adding it to the mixture of step 1).

Furtherance Solubilization Formulation 1 Solubilization Formulation 2 Solubilization Formulation 3 One Purified water ~ 100 ~ 100 ~ 100 2 Concentrated glycerin 3 3 3 3 1,3-butylene glycol 2 2 2 4 EDTA-2Na 0.01 0.01 0.01 5 Pigment 0.0001 0.0002 0.0002 6 The cosmetic composition of Example 1 0.1 5 5 7 Alcohol (95%) 8 8 8 8 Methyl paraoxybenzoate 0.1 0.1 0.1 9 Polyoxyethylene
Hydro genide ester 0.3 0.3 0.3 10 incense 0.15 0.15 0.15 11 Cyclomethicone - - 2

Claims

(A) After mixing the beneficial microorganisms and mineral concentrated water which are the solution of green tea leaf, green tea leaf, Gucci mulberry, obtusa, Saururus chinensis, Omija, silver mushroom, ginseng, brown rice, Primary fermentation step;
(B) injecting air under the same temperature as that of the primary fermentation to perform secondary fermentation;
(C) Cereal, lemon, Cheongpiae, antler, white mulberry, rhubarb, savannah, rye, oats, rice, black beans, apricot seeds, Condensing mineral water and deep sea water to extract hot water;
(D) separating the fermented product fermented in step (B) into a fermentation broth and a residue;
(E) hot water extraction of the residue separated in step (D); And
(F) mixing the fermentation broth separated in the step (D), the extract extracted in the step (E) and the extract extracted in the step (C). (EN) The present invention relates to a method for producing a cosmetic composition for improving skin wrinkles or skin moisturizing using a substance.

[7] The method according to claim 1, wherein 50 to 600 parts by weight of green tea leaves, guacamole fruit, obtusa, saury, omija, silver mushroom and ginseng are added to 100 parts by weight of the feed in step (A) 5 to 10 parts by weight of brown rice; 5 to 10 parts by weight of yeast; 100 to 300 parts by weight of crude oil; 100 to 300 parts by weight of useful microorganisms; 20 to 100 parts by weight of mineral concentrated water; 1500 to 2000 parts by weight of deep ocean water; And 1500 to 2000 parts by weight of a yellowing agent. The method for manufacturing a cosmetic composition for improving skin wrinkles or skin moisturizing using a naturally-occurring material comprising a wort.

The method according to claim 1, wherein the primary fermentation is performed at 20 to 50 ° C for 12 to 20 days in the step (A). The cosmetic composition for improving skin wrinkles or skin moisturizing using a natural- &Lt; / RTI >

The method according to claim 1, wherein the secondary fermentation is performed for 12 to 20 days in step (B).

The method according to claim 1, wherein in step (C), 100 to 250 parts by weight of the lashes are added to 100 parts by weight of the feedstock; 100 to 2000 parts by weight of a group consisting of green tea, green tea, green tea, white sage, rhubarb, savannah, rye, oats, rice bran, black beans, apricot seeds, corn oil, 30 to 100 parts by weight of a menthol; 300-1000 parts by weight of birch sap; 20 to 50 parts by weight of mineral concentrated water; And 1000 to 20,000 parts by weight of deep-sea water. The method of manufacturing a cosmetic composition for improving skin wrinkles or skin moisturizing using a natural-derived material.

The method of claim 1, wherein the hot-water extract in step (C) is extracted from the mixture at 70 to 90 ° C. for 100 to 130 hours. The cosmetic composition for improving skin wrinkles or skin moisturizing using natural- &Lt; / RTI >

The method of claim 1, wherein the hot water extract in step (E) is a mixture of residue and water at a weight ratio of 1: 1 to 3 and is extracted at 70 to 90 ° C for 10 to 30 hours. A method for manufacturing a cosmetic composition for improving skin wrinkles or skin moisturizing using a derived material.

2. The method according to claim 1, wherein the fermentation broth separated in step (D), the extract extracted in step (E), and the extract extracted in step (C) Wherein the cosmetic composition is mixed at a weight ratio of 3.0 to 3.0.

A cosmetic composition for improving skin wrinkles or skin moisturizing, which is produced by the manufacturing method according to any one of claims 1 to 8.

The composition according to claim 9, wherein the composition is selected from the group consisting of a solution, an external ointment, a cream, a foam, a nutritional lotion, a softening water, a softening water, an emulsion, a makeup base, an essence, a soap, Wherein the cosmetic composition has a formulation selected from the group consisting of pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations and wax foundations.