KR102085559B1 - Tremella fuciformis extraxt, preparation method thereof and Use the same - Google Patents

Abstract

The present invention relates to a composition for promoting filaggrin and involukrin production comprising silver polysaccharide mushroom as an active ingredient.

Description

Silver mushroom extract, preparation method thereof and use thereof {Tremella fuciformis extraxt, preparation method about and use the same}

The present invention relates to a silver mushroom extract, a preparation method thereof and its use, and more particularly, to a silver hot mushroom extract, a preparation method thereof, and a use as a cosmetic composition, which are effective for promoting the production of involuclin and filaggrin.

Tremella fuciformis is a plant belonging to the white family of the family, and its origin is China, Japan, Korea, etc., and it occurs on rotten trees in hilly areas and mountain hardwoods. The fruiting body is 3-8 cm in diameter, similar to pure white, translucent gelatin, and is called silver because of its shape similar to the human ear. According to traditional Chinese medicine theory and Chinese ancient Chinese medicine, silver mushrooms were used not only for the treatment of chronic bronchitis, but also as a beauty food during the sugar and song era.

Silver mushrooms contain minerals such as calcium and iron, and contain 500 times more moisture than their own weight. It is also used as a raw material for cosmetics because it has the effect of reducing wrinkles and preventing fine wrinkles by preventing aging of skin microvascular vessels. Silver mushrooms are known to contain trehalose and collagen, the sugars produced by fungi, and vegetable oils and polyphenols, which are antioxidants.

Republic of Korea Patent Publication No. 1516384 discloses a configuration in which the white-necked mushroom extract is prepared in a gel or solution state and nanoparticles are used as an additive of a cosmetic composition as a thickener, and Republic of Korea Patent Publication No. 10-2011-0052095 Discloses a skin moisturizing cosmetic composition containing mushroom extract and hyaluronic acid as an active ingredient, and is prepared by using enzymes in Korean Patent Publication No. 1257993 for nerve cell recovery, nerve cell damage suppression, and memory enhancement. Disclosed is a configuration to use, in the Republic of Korea Patent Publication No. 10-2005-0059956 has been disclosed a composition used for preventing dementia mixed with five natural materials. In addition, Park et al. Proposed the possibility of using the hot water extract of silver mushroom as a preparation for degenerative neurological diseases such as Alzheimer's disease (Mycobiology 35 (1): 11-15, 2007), and Kim et al. The antioxidant activity of the free radical scavenging activity was measured (Food Engineering Progress 15 (1), 6-14, 2011.02).

The problem to be solved by the present invention is to provide a composition comprising a mushroom extract of silver is effective in promoting the production of filaggrin and involukrin and a preparation method thereof.

Another object of the present invention is to provide a use of the silver mushroom extract.

In order to achieve the above object, the present invention provides a composition for promoting the production of involuclin and filaggrin containing silver mushroom hot water extract.

In the present invention, the silver mushroom may be fermented with fungi.

In the present invention, the fungus may be a fungus strain of the genus Aspergillus.

In the present invention, the fungus may be selected from the group consisting of Aspergillus niger, Aspergillus duckwood and Aspergillus awamori.

In the present invention, the hydrothermal extract may be one to four days after the hydrothermal extraction with one or more selected from pectinase, cellulase, hemicellulase, beta glucanase, arabinase and xylanase have.

In the present invention, the composition may be to increase the skin stratum corneum thickness.

According to the present invention, the composition may include 3-7% by weight of galactose, 8-15% by weight of glucosamine and 10-20% by weight of galactosamine with respect to 100% by weight of the composition solids.

In addition, the present invention provides a method for producing a silver mushroom hot water extract, comprising the steps of slicing the mushrooms and extracting the hot water, filtering and drying the silver.

In addition, the present invention 1) sterilized silver is inoculated fungi to mushrooms, and the step of fermenting at 27-37 ℃ for 2-4 days; And 2) extracting the fermented silver mushrooms from hot water for 5 to 24 hours in water at 80 to 110 ° C .;

According to the present invention, the step 2) is to treat the hydrothermal extract 1-4 days with at least one enzyme selected from pectinase, cellulase, hemicellulase, beta glucanase, arabinase and xylanase It may further include.

In addition, the present invention provides a cosmetic composition for improving one or more symptoms selected from the symptoms of skin aging, dermatitis, skin elasticity lowering, wrinkles, erythema, skin moisturizing lowering and thinning of the stratum corneum including the silver hot mushroom extract.

In the present invention, the skin aging may be at least one selected from skin photoaging and skin degradation.

Silver ear mushroom extract according to the present invention, enzyme treatment of silver ear mushroom extract and solid fermented silver ear mushroom extract promotes the production of filaggrin and involucine which are involved in skin differentiation, so that the skin damaged by various factors such as ultraviolet rays or trauma Can promote regeneration. In addition, since there is no phototoxicity, there is no skin sensitization and eye irritation, it can be usefully applied industrially.

1 is a sugar component analysis of the silver polysaccharide mushroom according to Example 1 of the present invention.
2 is a molecular weight analysis of the silver polysaccharide mushroom according to Example 1 of the present invention.
Figure 3 is an amino acid analysis of the silver polysaccharide mushroom according to Example 1 of the present invention.
Figure 4 is a result of analysis of involukrin protein expression of silver polysaccharide mushroom according to Example 1 of the present invention.
5 is a filaggrin protein expression analysis of the silver polysaccharide mushroom according to Example 1 of the present invention.
Figure 6 is a TNF-α expression analysis of the silver polysaccharide mushroom according to Example 1 of the present invention.
7 is a result of confirming the inhibitory effect of collagen degrading enzyme expression of aging skin fibroblasts induced by the heat of the mushroom polysaccharide according to Example 1 of the present invention through the inhibition of MMP-1 production.
8 is a collagen synthesis result of the silver polysaccharide mushroom according to Example 1 of the present invention.
9 is a result of inhibiting the generation of nitrogen oxide of the silver polysaccharide mushroom according to Example 1 of the present invention.
Figure 10 is the result of measuring the erythema reduction effect of the silver mushroom polysaccharide according to Example 1 of the present invention.
11 is a result of measuring the wrinkle improvement effect of the silver mushroom polysaccharide according to Example 1 of the present invention.
12 is a result of measuring the elasticity improvement and moisturizing improvement effect of silver mushroom polysaccharide according to Example 1 of the present invention.
13 is a skin stimulation test result of the silver mushroom polysaccharide according to Example 1 of the present invention.
14 is an allergy test result of the silver mushroom polysaccharide according to Example 1 of the present invention.

The skin covers the outside and is a body organ composed of the epidermis, the dermis and the subcutaneous fat layer. Involuclin is a soluble cytosolic protein that is expressed in the granular layer of the epidermis and the upper part of the epidermis. It is an important biomarker for skin differentiation that is expressed early in the end stages of keratinocyte differentiation. Filaggrin is a histidine that acts as a substrate that binds to proteins. It is a kind of abundant protein and is a biomarker that plays an important role in maintaining and differentiating moisture content of epidermis. The inventors of the present invention have developed a modulator that can control biomarkers associated with skin to complete the present invention.

Hereinafter, the present invention will be described in more detail.

The present invention provides a composition for promoting the production of involuclin and filaggrin containing silver mushroom hot water extract.

The silver mushroom hot water extract according to the present invention can promote the production of involuclin and filaggrin at the same time.

According to one embodiment of the invention, the silver mushroom hot water extract may be a precipitate obtained by treating the hot water extract of silver mushrooms with alcohol.

The silver mushroom extract may comprise a polysaccharide, the polysaccharide may be to act to promote the production of involuclin and filaggrin.

According to one embodiment of the invention, the silver mushroom may be fermented with fungi, the fungus is preferably Aspergillus genus ( Aspergillus) spp . ) May be a fungal strain, more preferably selected from the group consisting of Aspergillus niger, Aspergillus duckwood and Aspergillus awamori, and most preferably Aspergillus niger.

According to the present invention, when fungi, especially silver fermented with Aspergillus strains, are hydrothermally extracted using mushrooms, they promote the production of involuclin and filaggrin more than those fermented with other fungi, and promote collagen biosynthesis. Silver mushrooms obtained hydrothermal extracts with increased and decreased inflammatory markers.

According to the present invention, the fermentation is not particularly limited as long as it is a fungal fermentation method that is commonly used, preferably solid phase fermentation. When silver fungal fermented mushrooms, the composition of the polysaccharide in the extract may be different, and it was confirmed that the ratio of the polysaccharide having a low molecular weight, which is effective in promoting the production of involuclin and filaggrin, was increased.

According to one embodiment of the present invention, the hydrothermal extract is one or more enzymes selected for 1-4 days from pectinase, cellulase, hemicellulose, beta glucanase, arabinase and xylanase after hot water extraction. It may be processed.

When the hydrothermal extract was treated with an enzyme, the composition of the polysaccharide in the extract may vary, and it was confirmed that the ratio of the polysaccharide having a low molecular weight effective in promoting the production of involuclin and filaggrin was increased.

The composition according to the invention may be to increase the thickness of the stratum corneum by promoting involukrin and filaggrin production, to prevent lowered skin moisturization, to promote collagen production, to repair or regenerate damaged skin tissue.

According to the present invention, the composition may include 3-7% by weight of galactose, 8-15% by weight of glucosamine and 10-20% by weight of galactosamine with respect to 100% by weight of the composition solids.

In addition, the present invention provides a method for producing a silver hot mushroom extract, which comprises the step of extracting the hot water mushrooms, filtering and drying the silver.

According to the present invention, the silver mushroom may be used in a state of being collected, but may be dried. The drying may be dried by conventional drying methods, for example, may be reduced pressure or lyophilization, preferably lyophilization.

In addition, the silver may be shredded or powdered to improve the extraction yield.

According to the present invention, the hot water extraction may be obtained by extracting the silver mushroom for 2 to 12 hours at 80 to 110 ℃. It is preferable that the temperature range is obtained because an extract in which the content of the useful component is maximized can be obtained. The obtained extract can be filtered to remove impurities. Filtration may be filtered by selecting the filter cloth according to the mushroom processing state of silver, for example, it may be filtered using a filter cloth of 200 to 800 mesh. In addition, it can be further purified using high performance filters such as micro filters to further increase the content of oil soluble components and minimize the content of impurities. The micro filter may be a filter in the range of 0.3 to 1 μm, and the present invention was filtered using a 0.45 μm filter.

According to the present invention, it can be extracted using 20 to 50 parts by weight of water with respect to 1 part by weight of silver mushrooms. The above range is preferable because it is possible to obtain an extract in which the content of the useful component is maximized.

In addition, the present invention 1) sterilized silver is inoculated fungi to mushrooms, and the step of fermenting at 27-37 ℃ for 2-4 days; And 2) extracting the fermented silver mushrooms from hot water for 5 to 24 hours in water at 80 to 110 ° C .;

According to the present invention, the step 2) is to treat the hydrothermal extract 1-4 days with at least one enzyme selected from pectinase, cellulase, hemicellulase, beta glucanase, arabinase and xylanase It may further include.

In addition, the present invention provides a cosmetic composition for improving one or more symptoms selected from the symptoms of skin aging, dermatitis, skin elasticity lowering, wrinkles, erythema, skin moisturizing lowering and thinning of the stratum corneum including the silver hot mushroom extract.

In the present invention, the skin aging may be skin damage (skin photoaging) by a light source such as ultraviolet light or skin damage (skin degradation) due to a high temperature outside the biocompatibility temperature, for example, 40 to 45 ℃ It is not limited to this.

The composition according to the present invention can increase the regeneration or activity of cells damaged by a light source such as ultraviolet light and / or cells damaged by heat outside the biocompatible temperature, and thus is useful as a cosmetic composition for improving skin aging.

In addition, the composition according to the present invention may increase collagen synthesis rate of skin cells and inhibit collagen degrading enzyme expression. Therefore, the composition of the present invention is useful as a cosmetic composition for preventing skin elasticity lowering and / or improving wrinkles.

In addition, the compositions according to the invention can relieve physical inflammation, such as inflammation caused by chemical inflammation and / or ultraviolet stimulation, such as inflammation induced by LPS, for example, and can cause erythema and / or inflammation caused by ultraviolet light. It can reduce or alleviate skin attacks.

In addition, the composition according to the present invention may exhibit an effect on skin moisturizing, wrinkle improvement, skin elasticity improvement through the activity of the skin barrier factor and the activity of the skin moisturizing factor.

The cosmetics according to the invention can be prepared in any formulation as known, for example skins, emulsions, essences, lotions, body lotions, body gels, body essences, body cleansers, cleansing foams, cleansing creams, cleansing gels , Pack, massage cream, massage gel, nutrition cream, sleep cream and moisture cream may be selected from the group consisting of.

In addition, in the cosmetic composition of each formulation, other ingredients other than the colored rice bran fermentation may be selected and formulated according to the purpose of use or the characteristics of the formulation, and additives may be added in a range that does not degrade the effect as necessary for the formulation of the formulation. It may further include.

The additives include, for example, water, oils, surfactants, humectants, thickeners, fragrances, preservatives, neutralizers, emollients, skin protectants, skin conditioning agents, preservatives, antioxidants, free radical destroying agents, opacifying agents, stabilizers, It may be one kind or two or more kinds selected from the group consisting of bactericides, oxidation stabilizers, silicones, antifoams, vitamins, heavy repellents, polymerizers, propellants, basicizing agents, acidifying agents, colorants, pigments and fillers, and the like. no.

Hereinafter, the present invention will be described in more detail with reference to preferred examples. However, these examples are intended to illustrate the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited thereby.

Example 1 Silver Mushroom Extract

100 g of fine dried silver mushrooms (Tremella fuciformis) and 3.5 kg of water were placed in an extractor, extracted at 100 ° C. for 8 hours, filtered through a 400 mesh filter cloth, and filtered through a 0.45 μm filter to obtain 2.1 kg of a filtrate solution. It was lyophilized to give 3.8 g of solid.

Example 2 Enzyme Treatment Silver Mushroom Extract

The silver prepared in Example 1 was added 200 g of pectinase (TCI, Japan) to 2 kg of the mushroom filtration aqueous solution and subjected to enzymatic treatment at 65 ° C. for 72 hours. The reaction was stopped, first filtered with a 400 mesh filter paper, and secondly filtered with a 0.45 μm filter to obtain 1.6 kg of filtered aqueous solution. It was lyophilized to give 2.4 g of solid content.

Example 3 Solid Fermentation Silver Mushroom Extract

The dried dried silver mushroom (Tremella fuciformis) was added 2.0 kg of water, sterilized first, and then treated with Aspergillus niger separated from the yeast to 1.0 × 10 ⁸ level. Next fermented at 30 ℃ for 60 to 64 hours, and then sterilized in an autoclave. Fermented product and 3.5 kg of water were placed in an extractor, extracted for 8 hours at 100 ° C., filtered first with 400 mesh filter paper, and filtered with 0.45 μm filter for 2 hours to obtain 2.8 kg of an aqueous solution. It was lyophilized to obtain 3.3 g of solid.

Test Example 1. Component Analysis

Sugar ingredient analysis

Bio LC (Dionex, USA) was used for sugar component analysis of the extracts of Examples 1 to 3, using a CarboPac PA10 (4 × 250 mm, Dionex) column and analyzed through an ED50 with integrated amperometry detector. The mobile phase used 16 mM NaOH and the flow rate was 1.0 mL / min.

As shown in Table 1 and Figure 1, the extract of Example 1 was confirmed that the main component is composed of β-glucan, in addition to containing mannose and fucose. In Examples 2 and 3, the contents of fucose, glucose and mannose were decreased, and the contents of galactose, glucosamine and galactosamine were increased.

ingredient Example 1 (%) Example 2 (%) Example 3 (%) Fucoose 2.98 2.18 1.91 Glucose 0.70 0.60 0.50 Manno Ozu 29.30 24.20 19.34 Galactose 0 1.64 4.20 Glucosamine 0.03 8.54 10.50 Galactosamine 0.00 6.41 15.30

Molecular Weight Analysis

The molecular weight of the extract of Example 1 was measured. As shown in Figure 2, the extract was mainly composed of a polysaccharide having a molecular weight of about 500,000 Da, in addition to the low molecular oligosaccharides were confirmed to contain.

Amino acid analysis

The molecular weight of the extract of Example 1 was measured. As shown in Figure 3, the extract includes aspartic acid, glutamic acid, serine, glycine, histidine, arginine, threonine, alanine, proline, tyrosine, valine, methionine, cysteine, isoleucine, leucine, N-leucine, phenylalanine, lysine It was confirmed that various amino acids, such as contained.

Test Example 2 Evaluation of Biological Activity

Test Example  2.1 Cell Culture

HaCaT cells were aliquoted into a 60 mm dish and incubated for 24 hours in an incubator containing 5% carbon dioxide at 37 ° C. After 24 hours UV-B was irradiated with 20 mJ and the extracts of Examples 1-3 were treated with 0, 125, 250 and 500 ppm respectively and incubated for 24 hours. After culturing, cells and cultures were recovered and evaluated for physiological activity using the following methods.

Test Example  2.2 Western blot Involukrin ( Involucrin ) Protein Expression Analysis

The skin is composed of five layers: the stratum corneum, the clear layer, the granule layer, the polio and the basal layer. Involuclin is a soluble cytosolic protein component expressed in the granular layer of the epidermis and the upper part of the polarization, and is an important biomarker of skin differentiation expressed early in the end differentiation of keratinocytes.

After irradiating UV-B to HaCaT cells, the extracts of Examples 1 to 3 were treated with 0, 125, 250, and 500 ppm for 24 hours, followed by electrophoresis with the recovered protein. After transfer to nitrocellulose membrane, anti-involuclin is used as the primary antibody, HRP-conjugated anti-mouse antibody and HRP-conjugated anti-rabbit antibody are used as secondary antibodies, Expression was analyzed.

Sample concentration Example 1 Example 2 Example 3 0 ppm (UVB treatment X) 1.000 1.000 1.000 0 ppm (UVB treated O) 0.718 0.718 0.718 125 ppm 0.937 0.975 1.081 250 ppm 1.052 1.112 1.254 500 ppm 1.156 1.184 1.514

As shown in Table 2 and Figure 4, the extract according to the present invention has been shown to improve the activity of involukrin reduced by ultraviolet light, especially when treated at a concentration of 500 ppm, the extract of Example 1 Involukrin activity was increased by more than 47%, Example 3 confirmed that the extract is increased by more than 110%.

Test Example  2.3 using Western blot Pill-green ( Filaggrin ) Protein Expression Analysis

Filaggrin is a type of histidine-rich protein that acts as a substrate that binds to proteins and is a biomarker that plays an important role in maintaining and differentiating the epidermal moisture content.

Filaggrin protein expression was analyzed by the method of Test Example 2.2 using anti-pillagrin as the primary antibody instead of anti-involukrin.

density Example 1 Example 2 Example 3 0 ppm (UVB treatment X) 1.000 1.000 1.000 0 ppm (UVB treated O) 0.641 0.641 0.641 125 ppm 1.689 1.983 2.241 250 ppm 2.062 2.141 2.678 500 ppm 2.359 2.574 2.988

As shown in Table 3 and FIG. 5, the activity of filaggrin was decreased by ultraviolet rays, but the activity of filaggrin was increased by treatment of the extracts of Examples 1 to 3. When treated at a concentration of 500 ppm, the extract of Example 1 increased the pilagrin activity by 3.6 times or more, and the extract of Example 3 increased the pilagrin activity by 4.6 times or more.

The above results suggest that the silver mushroom extract according to the present invention increases the skin stratum corneum, thereby protecting the skin from external stimuli.

Test Example  2.4 TNF -α, IL-6 and MMP -One mRNA  Expression analysis

Analysis of Human TNF-α, IL-6 and MMP-1 mRNA Expression Using RT-PCR

After irradiating UV-B at 20 mJ / cm ² , the extracts of Examples 1 to 3 were treated with 0, 125, 250, and 500 ppm for 24 hours to incubate, and the cells were recovered and RNA was extracted using Trizol. . After synthesizing cDNA using the extracted RNA and RT PreMix, cDNA was performed by mixing PCR PreMix with primers of TNF-α, IL-6 and MMP-1. PCR products were analyzed by electrophoresis using 1.5% agarose gel.

Analysis of Human TNF-α, IL-6 and MMP-1 mRNA Expression by RT-qPCR

After irradiating UV-B at 20 mJ / cm ² , the extracts of Examples 1 to 3 were treated with 0, 125, 250, and 500 ppm for 24 hours to incubate, and the cells were recovered and RNA was extracted using Trizol. . After synthesizing cDNA using extracted RNA and RT PreMix, cDNA was mixed with Real Time PCR Master Mix and TaqMan probes of TNF-α, IL-6 and MMP-1, and then qPCR was run. Test results were analyzed by the ΔCt value.

Synthesis of Human TNF-α, IL-6 and MMP-1 by ELISA Assay

After irradiating with UV-B at 20 mJ / cm ² , the extracts of Examples 1 to 3 were treated with 0, 125, 250, and 500 ppm for 24 hours to incubate, and then cells and cell cultures were recovered to recover human TNF-α and human IL-. TNF-α, IL-6 and MMP-1 were synthesized using ELISA kits of 6 and human MMP-1, and protein levels were obtained from the cultured cells. The amount of synthesis of -1 was measured.

Table 4 shows the results of evaluation of the synthesis amount of TNF-α, Table 5 the synthesis amount of IL-6, and Table 6 the synthesis amount of MMP-1.

density Example 1 (%) Example 2 (%) Example 3 (%) 0 ppm (UVB treatment X) 100.00 100.00 100.00 0 ppm (UVB treated O) 148.59 148.59 148.59 125 ppm 123.31 131.44 121.49 250 ppm 121.66 123.56 110.65 500 ppm 113.44 110.22 100.61

density Example 1 (%) Example 2 (%) Example 3 (%) 0 ppm (UVB treatment X) 100.00 100.00 100.00 0 ppm (UVB treated O) 398.61 398.61 398.61 125 ppm 289.94 444.04 274.64 250 ppm 257.64 401.24 241.98 500 ppm 220.41 380.74 180.81

density Example 1 (%) Example 2 (%) Example 3 (%) 0 ppm (UVB treatment X) 100.00 100.00 100.00 0 ppm (UVB treated O) 130.74 130.74 130.74 125 ppm 105.68 101.24 102.45 250 ppm 98.28 94.75 91.24 500 ppm 81.06 78.21 75.66

As shown in Tables 4-6 and 6, the inflammatory response induced by ultraviolet light was inhibited by the extracts of Examples 1-3. When the extract of Example 1 was treated at a concentration of 500 ppm, the expression level of TNF-α was shown to be inhibited by five times as compared to the control (non-treated), and the extract of Example 3 was concentrated at 500 ppm. The experimental group treated with was found to inhibit most of the inflammatory response. The above results demonstrate that the silver mushroom extract according to the present invention has an excellent effect of alleviating skin irritation caused by ultraviolet rays.

Test Example  2. In five columns  Aging inhibitory effect

In order to demonstrate the improvement effect of the skin tissue damaged by heat, the extracts of Example 1 were treated with 0, 125, 250, 500 ppm of the dermal fibroblasts treated at 42 ° C. to inhibit collagen degrading enzyme expression of aging skin fibroblasts induced by heat. The effect was confirmed. As shown in FIG. 7, the concentration of MMP-1 was 70 ng / ml at 37 ° C., but the concentration of MMP-1 was increased at 42 ° C. FIG. On the other hand, according to the extract treatment concentration of Example 1, the concentration of MMP-1 was reduced, and when treated at a concentration of 500 ppm was reduced by about 20%.

Test Example  2.6 Measurement of Collagen Synthesis

After treating the extracts of Examples 1 to 3 with 0, 62.5, 125, and 250 ppm for 24 hours, cells and cell cultures were recovered to measure collagen synthesis amount.

density Example 1 (%) Example 2 (%) Example 3 (%) 0 ppm 100 100 100 62.5 ppm 112 116 118 125 ppm 115 120 124 250 ppm 120 125 138

As shown in Table 7 and FIG. 8, the amounts of collagen synthesis were found to increase as the silver concentration of the mushroom polysaccharides of Examples 1 to 3 increased, in particular, the silver was subjected to the solid phase fermentation pretreatment with the Aspergillus strain. When it was performed, it was confirmed that the amount of collagen synthesis can be further increased.

Test Example  2.6 LPS -Induced inflammatory response test

After inducing the inflammatory response with LPS, it was confirmed that the extract of Example 1 was treated with 0, 100, 300, 1000 ppm to inhibit the inflammatory response induced by LPS. As shown in FIG. 9, the inflammatory response was alleviated in a concentration-dependent manner, and when treated at a concentration of 1000 ppm, an inhibitory effect of about 28% was observed.

Test Example  2.7 Verification of erythema reduction effect

Nude rat models were divided into two groups, one group was treated with the extract of 5% Example 1, the other group was untreated and irradiated with UV light for 7 days to induce erythema. As shown in Figure 10, the extract of Example 1 was reduced by more than 10% erythema compared to the untreated population.

Test Example  2.8 Primos Wrinkle improvement effect

In order to confirm the wrinkle improvement effect, the extract of 5% Example 1 was treated for 8 weeks. As shown in FIG. 11, wrinkles were reduced by about 5.4% at week 8.

Test Example  2.9 Verification of elasticity improvement and moisture retention

5% of the extract of Example 1 was treated for 8 weeks to verify the effect of improving elasticity and moisturizing. As shown in Figure 12a, 12b, it was confirmed that the elasticity increased by about 5.1%, the moisturizing rate increased by 8.5%.

Through the above results, the silver silver mushroom extract, enzyme-treated silver mushroom extract and solid state fermented silver mushroom extract according to the present invention promotes the production of filaggrin and involukrin, increases collagen production, MMP-1, nitric oxide And by inhibiting the expression of inflammatory factors such as TNF-α it has been proved effective in preventing skin regeneration, moisturizing, soothing, elasticity, photoaging and thermal aging.

Test Example  3. Skin irritation test

Test Example  3.1 stimulation test

In order to test the skin irritation, the skin irritation was confirmed by treating the extracts of Examples 1 to 3 with 1%, 3%, and 5% for various age groups (20, 30, 40, 50s). The extracts of Examples 1 to 3 according to the present invention were all found to belong to the non-irritating family.

Test Example  3.2 allergy  Test

In order to test the skin irritation, the skin cumulative patch experiment was performed by treating with the extract of Examples 1 to 3 for various ages (20, 30, 40, 50, 60s). The extracts of Examples 1 to 3 according to the present invention were all found to belong to the allergy-free family.

Claims (12)

A composition for promoting the production of involukrin and filaggrin, wherein the fermented silver is fermented with Aspergillus niger . delete delete delete The method of claim 1,
The hot water extract is treated with 1 or more days selected from pectinase, cellulase, hemicellulase, beta glucanase, arabinase and xylanase after hot water extraction, involuclin and Composition for promoting filaggrin production. The method of claim 1,
The composition is a composition for promoting involukrin and filaggrin production, characterized in that to increase the skin stratum corneum thickness. The method of claim 1,
A composition for promoting involukrin and filaggrin production comprising 3-7% by weight of galactose, 8-15% by weight of glucosamine and 10-20% by weight of galactosamine relative to 100% by weight of the composition solids. 1 selected from skin photoaging, skin deterioration, dermatitis, skin elasticity lowering, wrinkles, erythema, skin moisturization and thinning of the stratum corneum, comprising the composition according to any one of claims 1 and 5-7. Cosmetic composition for improving symptoms more than species. delete 1) inoculating Aspergillus niger on sterilized silver mushrooms and fermenting at 27-37 ° C. for 2-4 days; And
2) extracting the fermented silver mushrooms in hot water for 5 to 24 hours in water of 80 to 110 ℃; manufacturing method of the silver mushrooms comprising the. The method of claim 10,
Further comprising the treatment of the hydrothermal extract of step 2) with at least one enzyme selected from pectinase, cellulase, hemicellulase, beta glucanase, arabinase and xylanase for 1-4 days, Method for preparing mushroom extracts. delete

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