KR20020084619A - Cosmetic composition containing phellinus linteus extract and vitis vinifera l. fermented solution and having anti-wrinkle effect - Google Patents

Abstract

PURPOSE: A cosmetic composition containing a Vitis vinifera L. extract and a Phellinus linteus extract to provide an effect greater than an additive effect of two components is provided which has an excellent skin elasticity-enhancing effect and antioxidant effect as well as collagen synthesis and is effective in prevention and improvement of wrinkle formation. CONSTITUTION: This cosmetic composition contains 0.01 to 20% by weight of an extract of Vitis vinifera L. and Phellinus linteus, wherein the Vitis vinifera L. extract and Phellinus linteus extract are mixed in a ratio of 1:0.01 to 1:20. The Phellinus linteus extract is obtained by adding the same volume of a mixed solution of ethanol, 1,3-butylene glycol, propylene glycol and distilled water as ground Phellinus linteus, heating at 85deg.C for 48hr and then filtering with a filter paper of 200 meshes. The Vitis vinifera L. extract is obtained by fermenting ground Vitis vinifera L. at 20 to 25deg.C for 6 to 8 days and then concentrating under reduced pressure.

Description

Cosmetic composition for anti-wrinkle containing a situation extract and grape fermentation extract {COSMETIC COMPOSITION CONTAINING PHELLINUS LINTEUS EXTRACT AND VITIS VINIFERA L. FERMENTED SOLUTION AND HAVING ANTI-WRINKLE EFFECT}

The present invention relates to a cosmetic composition having an anti-wrinkle effect, and more particularly to a cosmetic having an anti-wrinkle effect by containing a situation mushroom extract and a grape fermentation extract.

In Korea, an aging society is rapidly arriving as the medical and public health benefits are improved, and at the beginning of the 21st century, one in four people is expected to have an aging society in which 65 or older people are over 65 years old. As a result, the quality of life is being improved, and the desire for the maintenance of youth is increasing, and the demand for cosmetics, especially anti-wrinkle cosmetics, has increased.

As for the skin aging mechanism that is the basis of the development of anti-wrinkle cosmetics, the study of local exogenous photoaging, which is progressed by long-term ultraviolet exposure in parallel with systemic and endogenous physiological aging according to the increase of age, has been attracting attention. In particular, the effects of photoaging on the formation of wrinkles on the face, head, etc., which are important for beauty, have been shown to be great. As a basic research for the development of anti-wrinkle cosmetics, studies on photoaging and wrinkle formation using human skin or animal models are actively conducted. It is becoming. As for photoaging and wrinkle formation, many studies on basic physiological metabolic changes such as synthesis and degradation of collagen, which is a major component of skin, have been reported (Lavker RM, Cutaneous aging: chronological versus photoaging.In: Gilchrest BA) (ed). photodamage. USA: Blackwell science, Inc., 123-135 (1995).

Wrinkles can be said to be the morphology and structural changes of the skin that are locally formed and settled at the site where they are chronically modified by muscle movement. Examining the formation mechanism can be said to be important for understanding the actual wrinkle formation mechanism.

In this regard, as skin internal structure changes due to photoaging, epidermis, thickening of the dermis, accumulation of abnormal elastic material, or three-dimensional structure change of elastic fibers have been reported. In addition, for collagen called skeleton, which is a major component of skin structure, microfiberization and weakening of fibrous form of collagen fibers have been revealed (Zheng P. and kligman LH, uvA-induced ultrastructural changes in hairless mouse skin: A comparison to uv B-induced damage., J. Invest.dermatol., 100,194-199 (1993)).

However, the collagen fiber bundles formed by these bundles are known to have a great influence on the toughness of the skin through the study of the wound healing process, etc., but the effects on the structural change, the physical properties of the skin and the wrinkle formation due to photoaging There has not been enough review so far.

The mainstream of the recent skin aging research is the study of the direct and indirect effects of sunlight due to the destruction of the ozone layer, and the effects on the collagen fibers using various substances to measure the effect of wrinkles caused by the light. Is actively researching.

On the other hand, Vitis vinifera is a vine belonging to the genus Vitiaceae, which has been known to have urinary action, diarrhea, tonic action, metabolism promotion and lowering acidity of gastric juice. (Wonwon, 1984), wine has also been used for diarrhea and tonic, with appetite and tannin-rich red wine (7 Korean medicine revision, Moon Sungsa, 1998), and recent studies have reported that it prevents heart disease. It is becoming.

In addition, the situation mushrooms (phellinus linteus (Ikekawa, T.,: Antitumor action of some basidiomycetes, especially phellinus linteus., Gann, 59: 155, 1968)) are also known as phellinus Quel. em.lmaz), and the state mushroom in Korea refers to woody mud mushrooms (phellinus linteus). About 48 species of mud mushrooms are reported around the world, and in Korea, rust muddy mushrooms (P. ferruginosus), dry muddy mushrooms (P. gilvus), horse mud mushrooms (P. igniarius), and worm mushrooms (P. laevigatus), There are 8 species of P. pomaceus, P. robustus, P. pini, and P. mud. Its pharmacological action is known to be effective in the treatment of gynecological diseases such as anti-cancer effect, immune enhancing function, tumor suppression effect, uterine bleeding, menstrual impurity, etc. Used cosmetics are introduced.

In this regard, Republic of Korea Patent No. 206538 and Publication No. 99-81226, 99-30419, after extracting the situation mushroom extract from the artificial mushroom or natural situation mushroom, after the predetermined treatment process, the extract is A technique for preparing cosmetics in addition to cosmetics is disclosed.

However, as described above, cosmetics containing only situational mushroom extracts can be moisturized, antioxidant, and anti-aging due to the characteristics of the situational mushrooms, but the effect is not so excellent that virtually no big effect can be seen. There is a problem.

In this regard, the present applicants attempted to mix with various substances based on the situation mushroom extract in order to obtain a cosmetic having a more effective anti-wrinkle effect, by synthesizing the grape fermentation extract, synergistic effect more than the additive effect of the two components It has been found that can be obtained to complete the present invention.

Accordingly, it is an object of the present invention to provide a cosmetic composition having an antioxidant action and having an excellent anti-wrinkle effect.

In order to achieve the above object, according to the present invention, the mixture of the situation mushroom extract and grape fermentation extract in a weight ratio of 1: 0.01 to 1:20 is contained in an amount of 0.01 to 20% by weight relative to the total weight of the composition There is provided a cosmetic composition for preventing wrinkles.

Hereinafter, the content of the present invention will be described in more detail.

It is known that the situation mushroom extract has an anti-wrinkle effect, and grape extract is known to have antioxidant activity when used in large amounts. Surprisingly, however, when these different active ingredients are combined, synergistic effects exceeding the respective effects are obtained by blending a small amount of grape fermentation extract, as described below, rather than an additive effect of achieving two effects. The invention takes advantage of this effect. Its mechanism action is not clear, but in addition to the complementary action that the other side complements the part that is not covered by one mechanism action, some subtle correlations are thought to occur. do.

In order to obtain the situation mushroom extract of the present invention, it is preferable to use the situation mushroom belonging to the genus Mud mushroom (Phellinus Quel.em.Imaz) of the pine vinyl mushroom (Hymenochaetaceae).

Extract of the situation mushroom is obtained as follows. First, the situation mushrooms are dried until there is no change in weight, and then mechanically pulverized. An equal volume mixture of ethanol, 1.3-butylene glycol, propylene glycol, and distilled water is added to the pulverized product. This was treated for 24 hours at a high frequency with water bath at 85 ° C. for 48 hours and filtered through a 200 mesh filter cloth. The filtrate is separated by centrifugation, and the filtrate is sterilized and filtered with 0.45 μm filter paper to obtain a situation mushroom extract. At this time, the ratio of the pulverized product and the extraction solvent mixture is preferably a weight ratio of 1: 3, in this case, the situation mushroom extracts have a strong ultraviolet absorption capacity (200nm ~ 400nm) when analyzed by the ultraviolet absorption spectrum, Tests for application to skin cosmetics show an irritant value (0.5) that is significantly lower than the general organic UV blocker (stimulation level 2.0).

The grape fermentation extract used in the present invention, as well as other varieties (Vitis vinifera Linne), which is a common grape, can be any other varieties (Gebong, Pione, etc.), grapes without grapes (grape Peel, pulp, grape seed) is extracted by fermentation and ripening.

Grape extraction method is as follows.

Fermentation of pulverized grape fruit (grape peel, pulp and grape seed). At this time, a mixer or the like is used to grind the grape fruit. As said fermentation conditions, it is preferable to carry out for 6 to 8 days at 20-25 degreeC. The fermentation at 20-25 ° C is related to the fermentation capacity of the natural yeast on the skin, and if the temperature is below 20 ° C, the fermentation period becomes too long. Because there is. In addition, when the fermentation is carried out at the temperature for 6 to 8 days, all the sugars disappear and the fermentation is completed.

Subsequently, the filtrate obtained by filtering the pulverized product is aged, concentrated under reduced pressure, and spray dried. The aging is carried out continuously for 30 days at a constant temperature of 11 ℃, avoiding direct sunlight, it is preferable to continuously aging in a state where the temperature does not change. This is because the aged wine is easily oxidized when subjected to direct sunlight, and the wine, which is called a living organic substance, has a property that changes well with time, temperature, and light. In addition, if the temperature is too low, the aging is not properly, if the temperature is too high, it is not suitable for aging for a long time, so for the extraction of the active ingredient, the ripening temperature is preferably 11 ℃, the aging period is preferably 30 days. . Through this aging step, the active ingredient is eluted to the maximum.

It is effective to perform the said vacuum-concentration to 40 to 45 weight% with a vacuum evaporator. This is to shorten the process time when using a spray dryer. It is preferable to perform concentration under reduced pressure of 25-35 CmHg. This is because when the concentration is 25 CmHg or less, the concentration time is long, and when the concentration is 35 CmHg or more, a large amount of active ingredient is discharged.

The final extract obtained by concentration of the extract under reduced pressure using a spray dryer (inlet: 150-200 ° C., outlet: 80-100 ° C.) is a powdery grape fermentation extract.

By not using any of these solvents, glucose and fructose components in grapes are converted into alcohols, followed by fermentation and ripening to extract the active ingredient contents of flavonoids, vitamin C, vitamin P, amino acids, and catechins in grapes. This is significantly higher, and thus a much better anti-wrinkle effect can be obtained.

Mixing the situation mushroom extract and grape fermentation extract prepared by the above method will have an excellent anti-wrinkle effect. The mixing ratio of the situation mushroom extract and the grape fermentation broth extract is preferably in the weight ratio 1: 0.01 to 1:20, more preferably 1: 0.1 to 1:10. If the ratio of the grape fermentation extract is lower than 0.01, it is difficult to obtain a cell proliferation effect, and if it is higher than 20, the cell proliferation effect is hardly increased any more because it is uneconomical.

This mixture can be added to all cosmetics related to wrinkle prevention such as softening water, nourishing cosmetics, nourishing cream, massage cream, essence, pack, etc., the amount is preferably 0.01 to 20% by weight based on the total weight of the cosmetic composition, It is more preferable that it is 0.1-10 weight%. It is difficult to obtain the anti-wrinkle effect at a concentration of 0.01% by weight or less, and when blended at 20% by weight or more, it is uneconomical in terms of increasing the anti-wrinkle effect or in terms of stability of the product.

Sichuan mushroom extracts, grape fermentation broth extracts and mixtures thereof according to the present invention are described in the following examples. However, these examples are only for illustrating the present invention, and the scope of the present invention is not limited thereto.

Example

(Preparation of Grape Fermentation Broth Extract)

Crush 5 kg of grape fruit (mask bailey, grape skin, flesh, grape seed) into brewing barrel, ferment at 22 ℃ for 6 days, filter with 300 mesh filter cloth (Cheongggye Sangpo), and continuously 11 It was aged for 30 days at ℃. The matured product (3,750 ml) was concentrated under reduced pressure until concentrated to 40-45% with a Tokyo Rikakikai co.Rotary vacuum evaporator, dried using a spray dryer (model MH-8), and then powdered grape fermentation solution. 9.7 g of extract was obtained.

(Production of Situation Mushroom Extract)

As a situation mushroom, the same amount of P. linteus and deciduous fungi (P.pini), native to the Korean Peninsula, are mixed and dried in a cool place without the sunlight. It was ground by a mechanical method.

To 20 g of the pulverized mushrooms, 60 g of the same volume mixture (volume ratio 1: 1: 1: 1) of ethanol, 1.3-butylene glycol, propylene glycol and distilled water was added and the mixture was subjected to ultra high frequency treatment for 24 hours in a water bath at 85 ° C. for 48 hours. Filtration was carried out with a 200 mesh filter cloth (Cheongggye Sang Sang). The filtrate was separated by centrifugation at 3000 rpm for 10 minutes. The separated filtrate was sterilized and filtered with 0.45 ㎛ filter paper to obtain 51 g of mushroom extracts.

(Example 1)

The powdered grape fermentation broth extract and the situation mushroom extract were mixed in a weight ratio of 0.1: 1 using a stirrer (Songdang parts company, SSD-811A).

(Example 2)

The powdered grape fermentation broth extract and the situation mushroom extract were mixed at a weight ratio of 0.5: 1 using a stirrer (Songdang Parts Co., SSD-811A).

(Comparative Examples 1 and 2)

The powdered grape fermentation broth extract and the situation mushroom extract were prepared as Comparative Examples 1 and 2, respectively.

(Manufacture example 1)

Preparation of Soft Cosmetics

After separating and measuring the aqueous phase and ethanol phase according to the following composition, it was separately mixed, dissolved and mixed with putting the ethanol phase in the water phase. The additives were then added and mixed. This was filtered using a membrane filter (Samdeok Filtration Co., Paper filter) to prepare a flexible cosmetic water.

(Manufacture example 2)

Preparation of Nutrients

After dividing and measuring the aqueous phase and the oil phase according to the following composition, it was mixed and dissolved separately at 80 ± 2 ℃, the oil phase was mixed with the aqueous phase. Subsequently, the raw material 15 which is a neutralizing agent was added and neutralized, and it cooled to 50 degreeC. After cooling, the raw material 16 was added, and then cooled to 35 ° C. to prepare nutrient cosmetics.

(Manufacture example 3)

Preparation of Nutritional Cream

According to the following composition was prepared in the same manner as in Preparation Example 2.

(Manufacture example 4)

Preparation of Essence

According to the following composition, the raw materials were sequentially measured, mixed and dissolved, and then filtered through a 200 mesh sieve to prepare an essence.

Comparative Example 1

Essence was prepared in the same manner as in Formulation 1 of Preparation Example 4, except that 100% by weight of rose water was used instead of the grape fermentation extract of Comparative Example 1.

(Comparative Production Example 2)

Essence was prepared in the same manner as in Formulation 2 of Preparation Example 4, except that 100% by weight of rose water was used instead of the situation mushroom extract of Comparative Example 2.

(Comparative Production Example 3)

Essence was prepared in the same manner as in Formulation 3 of Preparation Example 4 except that the amount of rose water was used instead of the grape fermentation solution extract + situation mushroom extract of Example 1 to 100% by weight.

(Comparative Production Example 4)

Essence was prepared in the same manner as in Formulation 4 of Preparation Example 4, except that the amount of the rose fermentation extract + the situation mushroom extract of Example 2 was used to make 100% by weight.

Experimental Example 1

Collagen Fiber Biosynthesis Effect

The cells obtained directly from humans were treated with the extracts obtained in Comparative Examples 1 and 2, Examples 1 and 2 to measure collagen fiber biosynthesis effects. That is, human fibroblasts (Normal Human fibroblast: ATCC CCL-28) were put in a culture flask and cultured until 70-80% growth. Cells were cultured in 96-well plates at 1 × 10 ⁴ cells / 100 ul / tr and treated with 50 μl of the extract diluted with water (w / w) for 24 hours. After treating the extracts, the collagen fibers produced by commercially available collagen fiber biosynthesis measurement kit (Procollagen Type IC-peptide EIA kit: Takara MK101) were measured. At this time, the concentration of each extract added to the culture was 0.001%, 0.01%, 0.1%. The results were shown in Table 1.

The kit for measuring collagen fiber biosynthesis, using an antibody of collagen fiber, binds a chromophore showing a specific color to a site bonded to the collagen fiber, and measures the degree of color represented by the chromophore at 450 nm (model name: μQuant). , Bio-Tek instruments, Inc., and used the principle of measuring the biosynthetic collagen fibers.

density Collagen Island Biosynthesis Rate (%) Comparative Example 1 Comparative Example 2 Example 1 Example 2 0.001% by weight 105 107 125 141 0.01 wt% 110 118 158 180 0.1 wt% 117 120 189 199 Control 100 100 100 100 Comparison 69 69 69 69 The control group was not treated with the extract of Comparative Example 1, Comparative Example 2, Examples 1 and 2, and did not irradiate ultraviolet rays. The comparative group was compared with Comparative Example 1, Comparative Example 2, Example 1 and Example 2. Irradiated with ultraviolet light without treatment of extract.

As shown in the table, the grape fermentation extract and the situation mushroom extract is effective in the production of collagen fiber, but the effect is weak, the collagen fiber production effect that exceeds the respective effects by combining a small amount of grape fermentation extract in the situation mushroom extract Was obtained. Since the main factor of wrinkle formation is due to the reduction of collagen fibers, the increase in collagen fibers means that it can prevent wrinkles caused by aging, in particular photoaging.

Experimental Example 2

Skin elasticity promoting effect

35 healthy women (mean 28.5 years old) over 20 years old were divided into 5 groups under the conditions of 22-24 ° C and 55% relative humidity. The skin elasticity enhancing effect of the formulation 3 of Preparation Example 3 in Group C, the Formulation 4 of Preparation Example 3 in Group D, and the cosmetic composition of Comparative Preparation Example 3 in Group E were measured.

After using the cosmetics for 12 weeks (2 times / day) around the eyes, skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM575, Courage + Khazaka). The test results are shown in Table 2 below as ΔR5 values [R5 (12 weeks) -R5 (0 weeks)] as average values for each test group of the skin elasticity measuring device. R5 is a value indicating a net elasticity coefficient, the closer to 1, the better the elasticity.

Experimental group Skin elasticity enhancing effect (△ R5) A group 0.07 B group 0.10 C group 0.19 D group 0.21 E group 0.05

As shown in the table, the skin elasticity of Group D (Prescription 4 of Preparation Example 3) in which a small amount of grape fermentation extract was added to the situation mushroom extract was 33.33%, B compared to Group A (Prescription 1 of Preparation Example 3). It was 47.62% higher than the group (prescription 2 of Preparation Example 3) and 23.81% compared to the E Group (Comparative Preparation Example 3). Therefore, it was found that the cosmetics containing a small amount of grape fermentation extract in the situation mushroom extract effectively promotes skin elasticity.

Experimental Example 3

Antioxidative effect

This experiment compared the degree of automatic oxidation suppression using linoleic acid, which is well-oxidized.

3 ml of 10 mM linoleic acid solution and 0.075 ml of sample adjusted to 4 mg / ml were added to an 8 ml captube. 0.1 ml of the mixed solution was incubated at 37 ° C. for 24 hours, and then added to 4.7 ml of 75% ethanol and stirred. Subsequently, 0.1 ml of 30% ammonium thiocyanate solution and 0.1 ml of ferrous chloride reagent were added, and after 3 minutes, the absorption wavelength was measured at 500 nm, and the antioxidant effect was measured in comparison with the control without the sample.

sample Antioxidative effect Grape Fermentation Extract of Comparative Example 1 42.8 Situary Mushroom Extract of Comparative Example 2 46.9 Grape fermentation extract of Example 1 + situation mushroom extract (weight ratio 0.1: 1) 80.9 Grape fermentation extract of Example 2 + situation mushroom extract (weight ratio 0.5: 1) 82.1 Vitamin E 43.8

As shown in Table 3, the sample containing a small amount of grape fermentation extract to the situation mushroom extract, showed a very excellent antioxidant effect than the other samples including the grape fermentation extract and mushroom extract.

Experimental Example 4

Cell proliferation effect measurement

The cell proliferation effect was measured by treating the extract obtained in Comparative Examples 1 and 2 to the cells directly collected from humans by the weight ratio of the situation mushroom extract: grape fermentation extract shown in Table 4 below.

In other words, human fibroblasts (Normal Human fibroblast: ATCC CCL-28) were cultured in a culture flask (T-75 flask, TTP) until 70-80% growth. Cells were cultured in 96-well plates at 1 × 10 ⁴ cells / 100 ul / trough, and 50 μl of the extract diluted with water (w / w) was incubated for 72 hours. After 72 hours of incubation, each tank was treated with 50 μl of MTT [3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazolium bromide, sigma] reagent (2 mg / ml in PBS) for 2 hours and After incubation, the cells were measured at 560 nm (model name: μ Puant, manufacturer: Bio-Tek instruments, Inc.). As a control, no extract was added. The results were as shown in Table 4 below.

Dilution concentration Control A: B ¹⁾ = 1: 0.01 A: B ¹⁾ = 1: 0.1 A: B ¹⁾ = 1: 10 A: B ¹⁾ = 1: 20 One% 0.9385 1.1999 1.4573 1.9861 1.9917 0.1% 0.8830 1.1390 1.4510 1.9010 1.8645 0.01% 1.0100 1.1018 1.4098 1.8843 1.9042 0.001% 0.7435 0.8120 1.3986 1.8911 1.9250 0.0001% 0.9495 0.9547 1.5003 1.8000 1.9016 0.00001% 0.7545 0.7600 1.3691 1.7847 1.8954 The control was not treated with the extract. ¹⁾ A: B = Situation of mushroom extract: Weight ratio of grape fermentation extract.

In Table 4, when the weight ratio of the natural situation mushroom extract: grape fermentation extract of 1: 0.01 was 0.6 to 26%, 1:10 to 85 to 114.8%, 1:20 to 89.4 to 118% showed a cell proliferation effect. As shown in the results, when the weight ratio of the situation mushroom extract and grape fermentation extract is 1: 0.01 to 1:20, it was possible to obtain a significantly superior cell proliferation effect compared to the case where the extract was not treated.

As described above, according to the present invention, as well as the collagen fiber biosynthesis effect and the skin elasticity enhancing effect and antioxidant effect than the conventional mushroom extract containing cosmetics, the situation mushroom extract and a small amount of grapes excellent in anti-wrinkle and improvement effect The cosmetic compound of the fermentation broth extract can be obtained.

Claims (1)

A cream composition for preventing wrinkles, wherein the mixture comprising the situation mushroom extract and the grape fermentation extract is contained in an amount of 0.01 to 20% by weight based on the total weight of the composition.