KR20200103347A - Composition for preventing or improving of skin wrinkle or skin photo-aging comprising fermented extract of Stevia rebaudiana and scoria as an active ingredient - Google Patents

Abstract

The present invention relates to a composition for preventing or ameliorating skin wrinkles or skin photo-aging comprising a fermented extract of Stevia rebaudiana and scoria as an effective ingredient and, more specifically, to a composition for preventing or ameliorating skin wrinkles or skin photo-aging comprising a fermented product obtained by inoculating Lactobacillus plantarum and Lactobacillus brevis into a mixture between a water extract of Stevia rebaudiana and a hot water extract of scoria and fermenting the resulting product as an effective ingredient. The cosmetic composition of the present invention comprises a fermented extract of Stevia rebaudiana and scoria as an effective ingredient so as to effectively prevent or ameliorate skin wrinkles or skin photo-aging.

Description

Composition for preventing or improving of skin wrinkle or skin photo-aging comprising fermented extract of Stevia rebaudiana and scoria as an active ingredient }

The present invention relates to a composition for preventing or improving skin wrinkles or skin photoaging containing stevia and volcanic stone extract and fermentation as active ingredients, and specifically, Lactobacillus planta in a mixture of the hot water extract of stevia and the hot water extract of volcanic stone Rum ( Lactobacillus plantarum ) and Lactobacillus brevis ( Lactobacillus brevis ) It relates to a composition for preventing or improving skin wrinkles or skin photoaging, containing a fermented product obtained by inoculation and fermentation as an active ingredient.

As human skin ages, wrinkles, blemishes, and freckles are generated, and its elasticity decreases. The causes of skin aging are intrinsic aging (natural aging) caused by the decrease in the number of fibroblasts and a decrease in their action, resulting in a decrease in the amount of fibroblasts (collagen) synthesized, and the loss of moisture in the skin. It is divided into photoaging by action.

The skin is composed of epidermis, dermis, and subcutaneous tissue from the outside. The epidermis plays a role in preventing the invasion of external stimuli or pathogens, and regulating body temperature and moisture. The dermis is made up of connective tissue, and it functions to keep the skin elastic and elastic due to collagenous fibers and some elastic fibers. The subcutaneous tissue is a layer of connective tissue, and the fat cells present here act as a buffer to preserve and protect heat. According to the research results, when comparing natural aging and photoaging skin, it was found that light-induced aging had a greater effect on skin aging. In the case of photoaging, compared to endogenous aging, thick and deep wrinkles as well as fine wrinkles occur a lot and have a characteristic that is observed early. In addition, collagen, a fibrous tissue that generally gives flexibility to the skin, has the greatest effect on the formation of skin wrinkles, and is a major component that accounts for about 70% of the dermal tissue. Synthesis of collagen fibers decreases rapidly as skin aging progresses, and wrinkles are created and deepened in the skin as collagen fibers are deformed by the external environment. Collagenase, a collagenase, is involved in the degradation of collagen.

Photoaging is caused by ultraviolet rays in sunlight, and when the skin is exposed to ultraviolet rays, oxygen free radicals are generated in the body. Free radicals cause damage to skin cells and tissues, increase melanin production, and keratinization of the epidermis. As the stratum corneum is damaged, the thickness of the stratum corneum increases, and the ability to produce collagen in the dermal layer decreases by ultraviolet rays, as a result of which the dermis layer becomes thin, resulting in thick and deep wrinkles.

Recently, in order to reduce skin irritation caused by chemical substances, products using natural products are being actively developed, and research is also being conducted to find materials that can improve skin health by reducing the causes of skin damage or aging.

On the other hand, Stevia (Stevia rebaudiana ) contains a low-calorie sweet-tasting ingredient in leaves or stems, so it is used as a diet sweetener. It has an ingredient called stevioside, so it tastes 200 to 300 times sweeter than sugar, and its calories are very low at 4 calories per gram, and it is a natural sweetener that is not toxic and is a necessity for diabetics and people who need diet. I can. Steviaside is well soluble in water or alcohol, has heat resistance, and is not toxic, so it is used as a sweetener for sherbers, chewing gum, soft drinks, and medicines.

Volcanic stone Scoria is a type of volcanic ash found only in Jeju Island, Korea, and is known to be produced when parasitic volcanoes (orum) are formed throughout Jeju, and the reserves of Jeju Island are estimated at 20 billion㎥. Volcanic stone pine is a natural material with the best functions with the ability to fight E. coli of 92-93% far-infrared radiation, 97% deodorization rate, and 99.9% antibacterial fungi in its natural state. Far-infrared rays penetrate the skin up to 3 to 5 mm and not only activate cell functions, but also promote blood circulation, strengthen metabolism, and improve tissue regeneration.

The inventors of the present invention have developed a method for producing substances that are beneficial to the skin by using such stevia and volcanic stone clusters, and this is used in cosmetics for use in maintaining skin health, particularly for preventing or improving skin wrinkles or skin photoaging. I wanted to apply.

Korean Patent Registration No. 10-0620854 Korean Patent Registration No. 10-1118156 Korean Patent Registration No. 10-1066676 Korean Patent Registration No. 10-0596316

The main object of the present invention is to provide a cosmetic composition exhibiting an excellent effect in preventing or improving skin wrinkles or skin photoaging by using stevia and volcanic stone clusters.

According to one aspect of the present invention, the present invention is a fermented product obtained by inoculating and fermenting Lactobacillus plantarum and Lactobacillus brevis in a mixture of the hot water extract of stevia and the hot water extract of volcanic rock It provides a cosmetic composition for preventing or improving skin wrinkles or skin photoaging containing as an active ingredient.

In the cosmetic composition of the present invention, the hot water extract of stevia is obtained by adding 10 to 30 times the weight of water to the pulverized stevia and extracting at 50 to 100°C, and the hot water extract of the volcanic stone is pulverized It is obtained by adding 10 to 30 times the weight of water to water and extracting under pressure at 110 to 130°C, and the mixture is a mixture of the hot water extract of the stevia and the hot water extract of the volcanic rock in a weight ratio of 1:2 to 2:1 One is preferred.

In the cosmetic composition of the present invention, the fermentation is preferably performed by shaking at 35 to 39°C at 50 to 200 rpm for 12 to 36 hours.

The cosmetic composition of the present invention can effectively prevent or improve skin wrinkles or skin photoaging by containing a fermented product extracted from stevia and volcanic stone as active ingredients.

1 shows a fermented product manufacturing process according to an embodiment of the present invention.
Figure 2 is a result of investigation of the effect on the expression of elastin, collagen, and MMP-3 according to the fermented product treatment of the present invention in a Western blot under the condition of irradiating the skin fibroblasts with UV.

The composition of the present invention contains a fermented product obtained by inoculating and fermenting Lactobacillus plantarum and Lactobacillus brevis in a mixture of the hot water extract of stevia and the hot water extract of volcanic rock as an active ingredient. It features.

In the present invention, it is determined that the stevia can be used in any part, but it is preferable to use at least one material selected from leaves and stems.

In the present invention, it is determined that any of the volcanic rocks can be used irrespective of the collection area, but it is preferable to use those collected from Jeju Island in Korea.

In the present invention, the hot-water extract of stevia can be obtained through a conventional extraction method for extracting useful components through a method of heating using water as a solvent from a plant material, but 10 to 30 times the weight of the stevia pulverized product It is preferable to add water and extract at 50 to 100°C. At this time, the extraction time is preferably 12 to 36 hours. More preferably, 15 to 25 times the weight of water is added to the pulverized stevia and extracted at 80 to 100°C for 20 to 30 hours to obtain. In addition, it is preferable to use in the state of an extract from which sludge is removed through filtration after extraction.

In the present invention, the hot water extract of the volcanic rock can be obtained through a conventional extraction method for extracting useful components through a method of heating using water as a solvent from a mineral material, but 10 to 30 times the pulverized volcanic stone It is preferable to obtain by adding weight of water and extracting under pressure at 110 to 130°C. At this time, the extraction time is preferably 5 to 30 minutes. More preferably, 15 to 25 times the weight of water is added to the crushed volcanic stone, and it is obtained by pressure extraction at 115 to 125°C for 10 to 20 minutes. In addition, it is preferable to use in the state of an extract from which sludge is removed through filtration after extraction.

In the present invention, the mixture of the hot-water extract of stevia and the hot-water extract of volcanic rock can be obtained by mixing the hot-water extract of stevia and the hot-water extract of the volcanic rock, wherein the mixing ratio is 1: 2 to 2: 1 It is preferable to set it as a weight ratio. More preferably, the mixing ratio is 1: 1.5 to 1.5: 1 weight ratio.

The mixture of the hot water extract of stevia and the hot water extract of volcanic rock is preferably sterilized before inoculation of the fermenting bacteria in order to prevent erroneous fermentation due to the growth of various bacteria that may occur during the fermentation process. At this time, sterilization may be performed using a conventional liquid sterilization method such as a filtration method using a micropore filter or a high temperature and high pressure heating method.

In the present invention, Lactobacillus plantarum and Lactobacillus brevis are used as fermenting bacteria, in which case it is preferable to use KCCM 12116, ATCC 14917, IFO 15891 or DSM 20174 as the Lactobacillus plantarum strain, and KCCM as the Lactobacillus brevis strain It is preferred to use 35464, ATCC 8287, IFO 3345 or DSM 20556.

In the present invention, the inoculation may be performed by adding the liquid culture solution of the fermenting bacteria to a mixture of the hot water extract of stevia and the hot water extract of volcanic rock. At this time, the liquid culture solution of the fermenting bacteria can be obtained by inoculating each fermenting bacteria into a liquid medium in which each fermenting bacteria can grow and culturing them. At this time, it is preferable to use a method of shaking culture for 12 to 36 hours at 35 to 39°C and 50 to 200 rpm in MRS liquid medium in order to increase fermentation efficiency. Lactobacillus plantarum and Lactobacillus brevis can be cultured separately and each culture solution can be added to the hot water extract. Also, even if the two strains are cultivated together, it does not adversely affect the growth of each other. It is also possible to use a culture solution obtained after culturing. When using a culture solution obtained by culturing two strains separately, it is preferable to add 1 to 3% (v/v), and when using a culture solution obtained by culturing two strains together, it is recommended to add 2 ~ 6% (v/v). It is desirable to increase fermentation efficiency.

In the present invention, the fermentation may be performed by maintaining a mixture of the hot water extract inoculated with the fermenting bacteria at a temperature at which the fermenting bacteria can grow and ferment for an appropriate time. Preferably, it is good to ferment by shaking for 12 to 36 hours at 35 to 39°C, 50 to 200 rpm. According to this, more efficient fermentation can be achieved. More preferably, it is good to ferment by shaking at 36 to 38°C and 100 to 140 rpm for 20 to 30 hours.

For more efficient fermentation, it is preferable to ferment by adding sugar and nitrogen source to the mixture of the hot water extract and inoculating the fermentation bacteria. Sugar, such as glucose, that can be used by the fermenting bacteria as a carbon source may be used, and the nitrogen source can be used as a nitrogen source by the fermenting bacteria such as yeast extract. When using glucose, it is preferable to add 0.1 to 1% (w/v) to the mixture of the hot water extract for efficient fermentation, and when using the yeast extract, 0.05 to 0.4% (w/v) to the mixture of the hot water extract Addition to v) is preferred for efficient fermentation.

In the present invention, the fermented product prepared through the above method may be used as it is, but it is preferable to use it by filtration to obtain a filtrate, and to powder it through concentration and lyophilization.

The cosmetic of the present invention may be prepared in any form conventionally prepared in the art, and may be prepared by adding the fermented product during the manufacturing process of the cosmetic. At this time, the amount of fermentation added may be appropriately selected in consideration of the type, shape, purpose, and manufacturing method of the cosmetic, for example, 0.1 to 100% by weight, preferably 1 to 50% by weight, based on the final cosmetic weight, More preferably, it may be 2 to 20% by weight.

Hereinafter, the present invention will be described in more detail through examples. Since these examples are for illustrative purposes only, the scope of the present invention is not to be construed as being limited by these examples.

[Example]

1. Preparation of the fermented product of the present invention

1-1. Stevia extract manufacturing

Stevia leaves and stems were washed with water, dried at room temperature, and pulverized to obtain 500 g of a coarse pulverized product. 100 g of the coarse pulverized product was immersed in 20 times the weight of purified water and extracted with low temperature hot water at 95° C. for 24 hours in a low temperature extractor. The obtained extract was filtered with Whatman #5 filter paper to remove microorganisms and sludge to obtain an extract, and the remaining raw materials were repeatedly extracted three times in the same manner and then cooled at room temperature.

1-2. Manufacture of volcanic stone pine extract

After washing the volcanic stone with water, it was dried at room temperature and pulverized to obtain 500 g of a coarse pulverized product. 100 g of the coarse pulverized material was immersed in 20 times the weight of purified water and extracted by high-temperature pressurization at 121° C. for 15 minutes in an autoclave. The obtained extract was filtered through Whatman #5 filter paper to remove the sludge to obtain an extract, and the remaining raw materials were repeatedly extracted three times in the same manner, and then cooled at room temperature.

1-3. Preparation of fermentation microorganisms

Lactobacillus plantarum ( Lactobacillus plantarum ) (KCCM 12116, ATCC 14917) and Lactobacillus brevis ( Lactobacillus brevis ) (KCCM 35464, ATCC 8287) were inoculated into MRS liquid medium, respectively, and cultured with shaking at 37°C at 120 rpm for 15 hours. The culture broth thus obtained was then used for fermentation.

1-4. Fermentation production

Mixing the stevia extract of 1-1 and the volcanic rock extract of 1-2 in a weight ratio of 1:1, and adding the yeast extract to 0.2% (w/v) so that glucose is 0.4% (w/v) Thus, a fermentation medium was prepared and sterilized at 121°C for 15 minutes. Here, the strain culture solution of 1-3 was added and inoculated to a final 2% (v/v) per strain culture solution, followed by shaking fermentation at 37° C. at 120 rpm for 24 hours. The fermented product thus obtained was filtered, concentrated, and freeze-dried through Whatman #5 filter paper, and then powdered and used in subsequent experiments.

2. Antioxidant power

2-1. DPPH radical scavenging activity

Using the fermented product of Example 1 as a sample, antioxidant activity was investigated through a DPPH radical scavenging experiment.

DPPH (1,1-diphenyl-2-picrylhydrazyl) is a very stable free radical in itself. It measures a dark purple compound that exhibits specific light absorption at 517 nm. It is an antioxidant with radical scavenging activity. Antioxidant activity can be measured due to quantitative decolorization by.

After adding 1 ml of 500 μM DPPH solution to 1 ml of each concentration sample, the mixture was stirred, and then reacted in a dark place at 37° C. for 30 minutes. The reaction solution measured the absorbance at 517 nm, and the following radical scavenging activity was calculated and shown in Table 1.

Free radical scavenging activity(%) = (1-A/B) x 100

A: sample added absorbance, B: sample-free absorbance

sample Concentration (mg/ml) Antioxidant power DPPH radical scavenging activity (%) IC ₅₀ (mg/ml) Fermented product of Example 1 0.10 8.80 0.687 0.25 19.69 0.50 38.07 1.00 71.25 Vitamin C 0.02 22.34 0.046 0.05 58.02 0.10 100 0.25 100

* IC50 value refers to the concentration of the sample required for 50% radical elimination.

As shown in Table 1, as a result of comparing the antioxidant power through radical scavenging power according to the DPPH method, the fermented product of the present invention exhibited excellent antioxidant power in a concentration-dependent manner.

2-2. SOD-like activity

Using the fermented product of Example 1 as a sample, antioxidant activity was investigated through an experiment for measuring SOD-like activity.

SOD assay Kit-WST was used to measure according to the manufacturer's instructions (manual), and the samples were put in a 96-well plate by concentration and WST-1(2-(4-iodophenyl)-3-(4-nitrophenyl)-5- (2,4-disulfophenyl)-2H-tetrazolium, mono-sodium salt) and xanthine oxidase working solution were added, incubated at 37°C for 20 minutes, and then absorbance at 450nm with a microplate reader. By measuring, the following active oxygen species scavenging ability was calculated and shown in Table 2.

Active oxygen species scavenging ability (%) = [1-(Test area absorbance/Control area absorbance)] x 100

sample Concentration (mg/ml) Active oxygen species scavenging ability (%)
Fermented product of Example 1 0.10 8.80 0.25 10.69 0.50 20.07 1.00 21.36

As shown in Table 2, as a result of examining the antioxidant power through SOD-like activity measurement, the fermented product of the present invention exhibited excellent antioxidant power in a concentration-dependent manner.

3. Cytotoxicity

Using the fermented product of Example 1 as a sample, cytotoxicity was investigated through the WST-1 assay method.

Dispense the CD986sk fibroblast cell line into a 96-well cell culture plate at a concentration of 1×10 ⁵ cells/well and incubate for 24 hours in IMDM medium containing 10% FBS and 1% penicillin streptomycin at 37°C and 5% CO₂. I did. After confirming that the cells were completely attached, and washed 12 times with PBS, the fermented product samples of Example 1 were respectively 12.5 µg/ml, 25 µg/ml, 50 µg/ml, 100 µg/ml, 200 µg/ml, 100 µl of each concentration of 400µg/ml was treated and incubated for 24 hours in a 5% CO₂ incubator at 37°C. At this time, the control group (-) was treated with only IMDM (no phenol red) and incubated in a 5% CO₂ incubator at 37°C for 24 hours. Blank was treated in the same manner as the control (-) to set the background control of the WST-1 solution. After washing the cells twice with PBS, excluding the blank, the WST-1 solution diluted 10-fold in IMDM (no phenol red) was treated with 100 µl/well and incubated for 50 minutes, and the absorbance at 450 nm was measured using a microplate reader. Measured. The composition of the blank working solution was treated by diluting 10 times sterilized water with IMDM (no phenol red) instead of the WST-1 solution. By measuring the absorbance, the following cell viability was calculated and shown in Table 3.

Cell viability (%) = [(absorbance of sample treated group/absorbance of no sample treated group)] x 100

sample Concentration (mg/ml) Cell viability (%)

Fermented product of Example 1 12.5 103.25 25 100 50 98.50 100 90 200 88 400 54

As shown in Table 3, the fermented product of the present invention showed almost no cytotoxicity with a cell viability of about 98% up to a concentration of 50 µg/ml, and no significant cytotoxicity even at a concentration of 200 µg/ml. However, it was found to be cytotoxic at a concentration of 400 μg/ml or more.

4. Effect of inhibiting the expression of collagenase (MMP-1)

The fermentation product of Example 1 was used as a sample to investigate the activity of inhibiting expression of Matrix methaloprotease-1 (MMP-1).

Among the components constituting the connective tissue of skin cells, collagen is a major constituent protein that accounts for 90% of the dry weight of the skin. Collagen degradation directly affects the elasticity of connective tissue and wrinkle formation. MMP-1 is a protease that acts specifically on collagen, and by inhibiting MMP-1 to reduce the breakdown of collagen, it can maintain the elasticity of skin tissue and prevent wrinkle formation.

Substances with MMP-1 inhibitory activity protect collagen and maintain mechanical properties to prevent elasticity and skin sagging, so the MMP-1 inhibitory activity of the sample is measured to improve wrinkles and elasticity. It can be evaluated as being useful in developing cosmetics for skin.

Skin fibroblast cells (CCD986sk fibroblast cell line) were dispensed at a concentration of 2×10 ⁵ cells/dish and cultured in a 5% CO₂ incubator at 37°C for 48 hours. After washing the cells with PBS, the fermented product of Example 1 diluted in IMDM (no phenol red) containing no FBS was 12.5 µg/ml, 25 µg/ml, 50 µg/ml, 100 µg/ml, and 200 µg /Ml, 400µg/ml, 3 ml each was added and incubated for 24 hours in a 5% CO₂ incubator at 37°C. Then, cells were lysed using Cell Lysis Buffer and used in Human MMP-1 ELISA kit assay (LSBio, LS-F4960).

100 µl of samples corresponding to the samples, standard, and blank were treated in a 96-well plate of the kit, and incubated at 37° C. for 1 hour. Detection reagent A was treated with 100µl each, washed three times, and detection reagent B was treated with 100µl each. After washing 5 times, 90 µl of TMB substrate solution was treated for 20 minutes, and the reaction was stopped with a stop solution, and absorbance was measured at 450 nm. As a control group, a comparative experiment was conducted using epigallocatechin gallate (EGCG).

MMP-1 expression inhibition rate (%) = [(absorbance of sample treated group/absorbance of no sample treated group)] x 100

sample Concentration (mg/ml) MMP-1 expression inhibition rate (%) Fermented product of Example 1 12.5 30 25 40 50 70 100 77 200 90 EGCG (control) 12.5 10 25 20 50 27 100 38 200 48

As shown in Table 4, the fermented product of the present invention is superior to epigallocatechin gallate used as a positive control, and has a better effect of inhibiting the expression of MMP-1 in skin fibroblasts, and this effect was found to be concentration dependent. This means that the fermented product of the present invention inhibits the expression of MMP-1 in skin fibroblasts, effectively inhibiting the formation of wrinkles in the skin or maintaining the elasticity of the skin.

5. Effect of inhibiting the secretion of collagenase (MMP-1) under UV irradiation conditions

Using the fermented product of Example 1 as a sample, the activity of inhibiting excessive secretion of MMP-1 from skin fibroblasts was investigated by UV irradiation.

UV was irradiated using a UV4X irradiation device (manufacturer Dodo B&P), and skin fibroblasts were prepared in the same manner as in Example 4. After removing the medium before UV irradiation, the serum component in the medium was removed by washing with phosphate buffered saline (PBS), and UV-B was irradiated with 50mJ/cm2. In order to measure the activity of MMP-1 secreted extracellularly, the fermented product of Example 1 was treated by concentration on skin fibroblasts, and UV-B was irradiated at 50 mJ/cm 2, and then the efficacy of the fermentation treatment was evaluated. The amount of MMP-1 secreted after 24 hours of treatment was diluted so that the final concentration of the fermented product was 12.5 µg/ml, 25 µg/ml, 50 µg/ml, 100 µg/ml, and 200 µg/ml, respectively. Evaluated. MMP-1 was evaluated by collecting the culture supernatant and using enzyme-linked immunosorbent assay (ELISA) kits (Biotrak, Buckinghamshire, UK), and each measured amount of MMP-1 was corrected to the total protein amount.

sample Concentration (mg/ml) MMP-1 (ng/ml) Fermented product of Example 1 12.5 37 25 35 50 17.5 100 12 200 11.8 UV B irradiation - 46

As shown in Table 5, the fermented product of the present invention has an excellent effect of inhibiting excessive secretion of MMP-1 by UV irradiation, and this effect was found to be concentration dependent. This means that the fermented product of the present invention can effectively inhibit skin photoaging and skin wrinkles caused by UV.

6. Effect on the expression of elastin, collagen and MMP-3 under UV irradiation conditions

Using the fermented product of Example 1 as a sample, the effect on the expression of elastin, collagen and MMP-3, which are closely related to aging, wrinkles, and elasticity of the skin under UV irradiation conditions, was investigated through Western blot.

UV irradiation, preparation of skin fibroblasts, and treatment of the fermented product were performed in the same manner as in Example 5, but the treatment concentration of the fermented product was 10 µg/ml, 50 µg/ml, 100 µg/ml, 200 µg/ml, and 300 µg /Ml, 500 µg/ml.

Total cells 24 hours after the fermentation treatment and UV-B irradiation of Example 1 were lysed in a RiPA buffer (Sigma-Aldrich Corp., St. Louis, USA). After centrifugation, the total protein amount of the cell lysate was measured using the Lowry method (BioRad Laboratories, Hercules, CA). An aliquot of the supernatant containing the same amount of protein (15 μg) was electrophoresed on a 10% SDS-PAGE gel and transferred to a nitrocellulose membrane (Amersham Pharmacia Biotech., England, UK), followed by 0.1% Tween 20 (TBS-T). ) Containing 5% skim milk dissolved in TBS for 1 hour and hybridized with a primary antibody (monoclonal mouse aniti-human antibody, Santa Cruz Biotech., Santa Cruz, CA, USA). All primary monoclonal antibodies were diluted with TBS-T in a 1:1000 ratio. The bound antibody was reacted with a secondary antibody, donkey anti-goat horseradish peroxidase (Jackson ImmunoResearch Lab., West Grove, PA, USA) diluted 1000 times for 1 hour at room temperature. The protein transferred to the nitrocellulose membrane was detected with Supersignal West pico chemiluminescence (Pierce Biotech., Rockford, IL, USA) and photographed with an X-ray film (Konica Co., Tokyo, Japan).

As a result, by treating the fermented product of the present invention as shown in FIG. 2, the levels of elastin and collagen were increased in a concentration-dependent manner up to the fermented product treatment concentration of 300 μg/ml. MMP-3 collagenase protein levels were decreased in a concentration-dependent manner by treating the fermented product of the present invention. This means that the fermented product of the present invention increases the expression of elastin and collagen and decreases the expression of MMP-3, thereby effectively inhibiting skin photoaging and skin wrinkles caused by UV.

Claims (3)

Skin wrinkles or skin photoaging containing fermented products obtained by inoculating and fermenting Lactobacillus plantarum and Lactobacillus brevis in a mixture of the hot water extract of stevia and the hot water extract of volcanic rock Cosmetic composition for the prevention or improvement of. The method of claim 1,
The hot water extract of stevia is obtained by adding 10 to 30 times the weight of water to the pulverized stevia and extracting at 50 to 100°C,
The hot water extract of the volcanic rock is obtained by adding 10 to 30 times the weight of water to the pulverized product of the volcanic rock and extracting under pressure at 110 to 130°C,
The mixture is a cosmetic composition, characterized in that the mixture of the hot water extract of the stevia and the hot water extract of the volcanic stone cluster in a weight ratio of 1:2 to 2:1. The method of claim 1,
The fermentation is a cosmetic composition, characterized in that made by shaking for 12 to 36 hours at 50 to 200 rpm at 35 to 39 ℃.

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