KR20200027140A - Cosmetic composition comprising natural fermented extracts and preparation method of the same - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a natural ferment extract and a production method thereof. More specifically, provided is a cosmetic composition with excellent antioxidant functions, moisturizing functions and elasticity by containing a red ginseng fermented extract using Kimchi-derived lactic acid bacteria, a soybean fermented extract using fast-fermented bean paste-derived Bacillus subtilis, and a rice fermented extract using rice wine yeast. Also provided is a production method thereof.

Description

Cosmetic composition comprising natural fermented extract and preparation method thereof {Cosmetic composition comprising natural fermented extracts and preparation method of the same}

The present invention relates to a cosmetic composition comprising a natural fermentation extract and a method for manufacturing the same, and more specifically, including a red ginseng fermentation extract using kimchi lactic acid bacteria, a soybean fermentation extract using Cheonggukjang gochobacteria, and a rice fermentation extract using rice wine yeast. An object of the present invention is to provide a cosmetic composition having excellent antioxidant ability, moisturizing ability, and elasticity, and a manufacturing method thereof.

Human skin undergoes various physical and chemical changes in the aging process, and its causes are largely divided into internal factors and external factors. Intrinsic factors include a decrease in the secretion of various hormones that regulate metabolism, and a decrease in the synthesis of biological components due to the decrease in the activity of immune cells, and external factors include the activation of free radicals in response to UV, stress, and environmental changes.

This skin aging occurs due to oxidation of lipids, proteins, polysaccharides, and nucleic acids, which are the main components of the skin, and in particular, excessive inflammatory reactions occur due to changes in the arrangement and content of proteins such as collagen, elastin, hyaluronic acid, fibronectin, and proteoglycan. Also. In addition, the skin thickness decreases due to aging of the skin, wrinkles, blemishes, freckles, blotch, etc., the color becomes dull, and the elasticity decreases, reducing external beauty and protecting the internal organs, the main function of the skin. It may be difficult to perform.

When the skin damage is further exacerbated, a situation such as deterioration of immune function due to DNA mutation, mutation, and cancer may occur, and thus, many studies have been conducted on substances capable of preventing and improving the skin aging.

In particular, in recent years, naturalist cosmetics without artificial elements have been in the spotlight, and naturalist cosmetics containing natural ingredients do not contain chemicals or contain relatively small amounts, and consumers who exhibit allergic reactions to chemical products It can be used and it can be said to be environmentally friendly because it uses less chemicals.

Among natural cosmetics, research and development of cosmetics containing natural material extracts that have undergone fermentation has been actively conducted, and among them, there is an increasing demand for cosmetics using natural materials using natural fermentation methods.

Registered Patent No. 10-1867308 (Registration on 2018.06.07)

In the present invention, to provide a cosmetic composition excellent in antioxidant capacity, moisturizing power and elasticity, including a fermented extract of red ginseng using kimchi lactic acid bacteria, a fermented soybean extract using Cheonggukjang gochobacteria and a rice fermented extract using makgeolli yeast, and a method for manufacturing the same.

One embodiment of the present invention for achieving the object as described above relates to a natural fermentation cosmetic composition comprising a fermentation stock solution, the fermentation stock solution includes red ginseng fermentation extract, soybean fermentation extract and rice fermentation extract.

At this time, the red ginseng fermented extract may be obtained by inoculating the red ginseng extract with Lactobacillus plantarum.

In addition, the soybean ferment extract can be obtained by inoculating soybeans with Bacillus subtilis.

The fermented rice extract may be obtained by inoculating rice with Saccharomyces cerevisiae.

The natural fermentation cosmetic composition may include 42 to 71% by volume of the fermentation stock solution and 29 to 68% by volume of the natural fermentation moisturizer.

The natural fermentation moisturizing agent can be obtained by fermenting sugar cane.

On the other hand, another embodiment of the present invention relates to a method for preparing a natural fermented cosmetic composition, wherein the method of production is inoculated with Lactobacillus plantarum to a red ginseng extract and fermented, followed by separating a red ginseng fermentation extract To, red ginseng fermentation extract extraction step; Inoculating Bacillus subtilis (Bacillus subtilis) on soybeans soaked in water, followed by fermentation, separating the soybean fermentation extract, soybean fermentation extract extraction step; Inoculating Saccharomyces cerevisiae on rice soaked in water and fermenting it, followed by separating the rice fermentation extract, extracting rice fermentation extract; Fermentation stock solution preparation step of mixing red ginseng fermentation extract, soybean fermentation extract and rice fermentation extract; And a natural fermentation cosmetic composition preparation step of mixing the fermentation stock solution and a natural fermentation moisturizer.

In the present invention, including a fermented extract of red ginseng using kimchi lactic acid bacteria, fermented soybean extract using Cheonggukjang gochobacteria, and rice fermented extract using rice wine yeast, it is possible to provide a cosmetic composition excellent in antioxidant ability, moisturizing power and elasticity and a method for manufacturing the same.

Prior to the following description in detail through the preferred embodiment of the present invention, terms or words used in the present specification and claims should not be construed as being limited to the conventional or dictionary meanings, meanings corresponding to the technical spirit of the present invention To be interpreted as

Throughout this specification, when a part “includes” a certain component, it means that the component may further include other components, not to exclude other components, unless otherwise stated.

Throughout this specification, "%" used to indicate the concentration of a specific substance, unless otherwise specified, solids / solids (weight / weight)%, solids / liquids (weight / volume)%, and liquid / Liquid means (volume / volume)%.

The present invention relates to a natural fermentation cosmetic composition and a method for manufacturing the same, wherein the natural fermentation cosmetic composition according to an embodiment of the present invention includes a fermentation stock solution, and the fermentation stock solution includes red ginseng fermentation extract, soybean fermentation extract and rice fermentation extract Includes.

At this time, the red ginseng fermented extract may be obtained by inoculating the red ginseng extract with Lactobacillus plantarum.

Red ginseng is processed to steam more than 4 years old, preferably more than 6 years old, and steamed ginseng with peel and dried to make the moisture content less than 14%. During the manufacturing process, the browning reaction is promoted to give a dark reddish brown color and processing. The prototype remains in a very solid shape afterwards.

In the process of manufacturing red ginseng, a number of new physiologically active ingredients that are beneficial to our body are produced, some of which are unique to red ginseng, not found in ginseng or white ginseng.

These unique ingredients of red ginseng include maltol, an anti-aging component, ginsenoside Rh3, an anti-inflammatory component, ginsenoside Rh2, an anti-tumor component, and ginsenoside Rg3, an inhibitory component for cancer cell metastasis. It is used in various fields for various purposes such as anti-tumor, anti-fatigue, mental stability, anti-inflammatory and anti-aging.

In addition, red ginseng contains the most types and the largest amount of saponins than other types of ginseng. These saponins are useful substances for various pharmacological actions and are used as skin preparations. Therefore, it is possible to suppress skin aging due to UV exposure, wrinkles and inflammation.

In particular, when fermenting red ginseng using microorganisms, the high molecular weight saponin components of red ginseng such as Rg1, Rh1, Rb1 and Rb2 are low-molecularized, and thus low-molecular saponins such as Rh1, Rg3, Rg5 + and Rk1 are increased. As such, when the saponin is reduced in molecular weight, the content of saponin increases by about 20 times, and the absorption rate of saponin by the human body increases by about 100 times or more, thereby improving the efficiency of red ginseng consumption.

Various microorganisms may be used during fermentation of red ginseng, but when fermented using Lactobacillus plantarum, which has a particularly high sugar decomposition rate as kimchi lactic acid bacteria isolated from kimchi, the molecular weight of saponin is lower than that of other microorganisms. Since the efficiency is excellent, the present invention uses a red ginseng fermented extract fermented with red ginseng extract with Lactobacillus plantarum.

Meanwhile, the fermented soybean extract may be obtained by inoculating soybeans with Bacillus subtilis.

Soybean is an important plant protein resource and is used as an effective treatment for obesity, diabetes, inflammation and cardiovascular disease. In addition, it has the effect of improving the skin moisturizing ability and improving the aging skin because it functions to suppress collagen and hyaluronan synthesis, the expression of matrix metalloproteinase (MMP), an enzyme that decomposes hyaluronic acid, and regulates fibroblast proliferation.

When such soybeans are fermented with Bacillus subtilis, the content of vitamin E increases, thereby exhibiting antioxidant efficacy, and the content of isoflavones increases, thereby promoting collagen production in skin tissue.

In addition, poly-gamma-glutamic acid, which has a moisturizing power and cohesive power more than 10 times higher than that of hyaluronic acid, is produced, thereby promoting the creation of natural moisturizing factors for skin such as pyrolicone carboxylic acid (PCA), lactic acid, and uronic acid, and stable pH balance of the skin Because it acts as a maintenance agent, it can maintain the immunity of the skin.

Meanwhile, the rice ferment extract may be obtained by inoculating rice with Saccharomyces cerevisiae.

Rice ferment extract contains various substances such as starch, protein, fiber, mineral, vitamin, alcohol and organic acid. Flavonoids, polyphenols and squalene, the main active ingredients of rice ferment extract, not only have antioxidant and anti-inflammatory effects, but also whitening effect Therefore, it can be used as a multifunctional skin preparation.

As described above, the fermented red ginseng extract, the fermented soybean extract and the fermented rice extract each exhibit unique efficacy, but when used together, the antioxidant and anti-inflammatory effects of the cosmetic composition, moisturizing power, whitening function are improved, and especially the skin There is an effect that the elasticity is significantly improved.

The preferred content of each fermentation extract to achieve this effect is 26-39% by volume of red ginseng fermentation extract, 21-44% by volume of soybean fermentation extract and 17-52% by volume of rice fermentation extract.

Particularly, when the content of the red ginseng fermentation extract is less than 26% by volume, the antioxidant and anti-inflammatory functions of the cosmetic composition are lowered, whereas when it exceeds 39% by volume, problems such as skin sensation and rash may occur. It is preferred to be included within.

In addition, when the content of the soybean fermentation extract is contained in excess of 44% by volume, it is preferable to be included within the above-mentioned range since stickiness may cause skin residual feeling and discomfort of the user.

On the other hand, the natural fermentation cosmetic composition of the present invention includes a fermentation stock solution containing a red ginseng fermentation extract, a soybean fermentation extract and a rice fermentation extract and a natural fermentation moisturizer, preferably, 48-87% by volume of the fermentation broth and a natural fermentation moisturizer 13 ~ 52% by volume.

The fermentation stock solution is added to provide effects such as antioxidant, anti-inflammatory, moisturizing, and whitening, but the fermentation stock solution itself is not sufficiently moisturizing, and natural fermentation moisturizing agents are used together to supplement the insufficient moisturizing effect.

When the content of the fermentation stock solution is less than the minimum range, or when the content of the natural fermentation moisturizer exceeds the maximum range, there is a problem that it is difficult to obtain an effective effect due to the fermentation stock solution, whereas the content of the fermentation stock solution exceeds the maximum range, or the natural fermentation When the content of the moisturizing agent is included below the minimum range, the moisturizing effect of the cosmetic composition is negligible, an unpleasant odor may be generated due to the content of the excessive fermentation stock solution, and severe irritation may be given to some users. It is preferred to use fermentation stocks and natural fermentation moisturizers.

The natural fermentation moisturizer used at this time is 1,3-butylene glycol obtained by natural fermentation of sugar cane, and has less skin irritation and better moisturizing power than 1,3-butylene glycol extracted using petroleum-based materials. , It has the effect of strengthening the skin barrier.

As described above, a natural fermentation cosmetic composition comprising a fermentation stock solution and a natural fermentation moisturizing agent may be included and used in a cosmetic composition having a formulation such as skin, lotion, and essence in 1 to 10% by volume.

When the natural fermentation cosmetic composition is used in an amount less than 1% by volume, the effect of anti-aging, anti-inflammatory, whitening, and elasticity enhancement due to the active ingredients contained in the natural fermentation cosmetic composition is minimal, and when it is included in excess of 10% by volume In, it is preferable to be included in the above range because side effects such as skin sensation and rash may occur due to excessive active ingredients.

At this time, as a component other than the natural fermented cosmetic composition in the cosmetic composition, the rose and green tea fermented water components fermented with yeast can be used together.

On the other hand, the manufacturing method of the natural fermentation cosmetic composition according to another embodiment of the present invention, after inoculating the red ginseng extract with Lactobacillus plantarum and fermenting it, separating the red ginseng fermentation extract, extracting the red ginseng fermentation extract ; Inoculating Bacillus subtilis (Bacillus subtilis) on soybeans soaked in water, followed by fermentation, separating the soybean fermentation extract, soybean fermentation extract extraction step; Inoculating Saccharomyces cerevisiae on rice soaked in water and fermenting it, followed by separating the rice fermentation extract, extracting rice fermentation extract; Fermentation stock solution preparation step of mixing red ginseng fermentation extract, soybean fermentation extract and rice fermentation extract; And a natural fermentation cosmetic composition preparation step of mixing the fermentation stock solution and a natural fermentation moisturizer.

First, the extraction step of the red ginseng fermentation extract is a step of inoculating the red ginseng extract with Lactobacillus plantarum and fermenting it, followed by separating the red ginseng fermentation extract.

At this time, the red ginseng extract used is hot water extracted from the selected red ginseng, and the red ginseng extract obtained through hot water extraction can be prepared by sterilization by heat treatment at a temperature range of 110 to 130 ° C. for 20 to 50 minutes.

The red ginseng fermentation broth can be prepared by inoculating the prepared red ginseng extract with Lactobacillus plantarum, a lactic acid bacterium extracted from kimchi, and fermenting for 40 to 52 hours at a temperature range of 25 to 30 ° C.

Subsequently, after extracting only the red ginseng fermentation extract from the red ginseng fermentation broth using an ultrasonic extractor, the supernatant obtained by centrifugation is recovered, and the final red ginseng fermentation extract can be obtained by deodorization and decolorization using activated clay.

Next, the soybean fermentation extract extraction step may be performed by inoculating Bacillus subtilis with soybeans soaked in water and fermenting it, followed by separating the soybean fermentation extract.

Soybeans used for fermentation in this step may be sterilized by heat-treating soybeans immersed in water for 12 to 20 hours after washing in a temperature range of 110 to 130 ° C for 20 to 50 minutes. Through such heat treatment, soybeans are sterilized as well as soybeans are soft and soft, so that fermentation efficiency can be improved.

The soybean fermentation broth can be prepared by inoculating Bacillus subtilis on soybeans prepared as described above and fermenting for 20-50 minutes at a temperature range of 35-40 ° C.

Thereafter, after extracting only the soybean fermentation extract from the soybean fermentation broth using an ultrasonic extractor, the supernatant obtained by centrifugation is recovered, and the final soybean fermentation extract can be obtained by deodorizing and decolorizing using activated clay.

Next, the step of extracting the rice fermentation extract may be performed by inoculating Sakcharomyces cerevisiae on rice called water and fermenting it, followed by separating the rice fermentation extract.

At this time, the rice used for fermentation may be used by sterilizing the washed rice by immersing it in water for 12 to 20 hours, followed by heat treatment at a temperature range of 110 to 130 ° C for 20 to 50 minutes. Through this heat treatment, it is not only sterilized, but also has the advantage of improving the fermentation efficiency due to the ripening of the rice.

Saccharomyces cerevisiae was inoculated into the rice thus prepared, fermentation was performed for 6 to 10 days at a temperature range of 25 to 30 ° C. to obtain a rice fermentation broth, and then the rice fermentation was performed using an ultrasonic extractor. After separating the rice fermentation extract from the culture solution, centrifugation is performed to recover the supernatant obtained through centrifugation, and the final rice fermentation extract can be obtained by deodorization and decolorization using activated clay.

Subsequently, a fermentation stock solution preparation step is performed by mixing the red ginseng fermentation extract, soybean fermentation extract and rice fermentation extract obtained through the above steps to prepare a fermentation stock solution.

At this time, the red ginseng fermentation extract is mixed with 26-39% by volume, soybean fermentation extract 21-44% by volume and rice fermentation extract 17-52% by volume, which can significantly improve the antioxidant and anti-inflammatory effects, moisturizing power, whitening function and skin elasticity. Fermentation stock solutions can be prepared.

Particularly, when the content of the red ginseng fermentation extract is less than 26% by volume, the antioxidant and anti-inflammatory functions of the cosmetic composition are lowered, and when it exceeds 39% by volume, problems such as skin sensation and rash may occur. It is preferably included.

In addition, when the content of the soybean fermentation extract is contained in excess of 44% by volume, it is preferable to be included within the above-mentioned range since stickiness may cause skin residual feeling and discomfort of the user.

Finally, in order to compensate for the insufficient moisturizing power of the fermentation stock solution, a step of preparing a natural fermentation cosmetic composition that mixes the fermentation stock solution and a natural fermentation moisturizer is performed. At this time, preferably, the fermentation stock solution may be mixed with 48-87% by volume and 13-52% by volume of the natural fermentation moisturizer.

When the content of the fermentation stock solution is less than the minimum range, or when the content of the natural fermentation moisturizer exceeds the maximum range, there is a problem that it is difficult to obtain an effective effect due to the fermentation stock solution, whereas the content of the fermentation stock solution exceeds the maximum range, or the natural fermentation When the content of the moisturizing agent is included below the minimum range, the moisturizing effect of the cosmetic composition is negligible, an unpleasant odor may be generated due to the content of the excessive fermentation stock solution, and severe irritation may be given to some users. It is preferred to use fermentation stocks and natural fermentation moisturizers.

The natural fermentation moisturizer used at this time is 1,3-butylene glycol obtained by natural fermentation of sugar cane, and has less skin irritation, excellent skin spread, and excellent skin affinity compared to butylene glycol extracted using petroleum-based materials. great.

Hereinafter, an embodiment of the present invention will be described. However, the scope of the present invention is not limited to the following preferred embodiments, and those skilled in the art can implement various modified forms of the contents described herein within the scope of the present invention.

[Production Example 1]

Preparation of red ginseng fermentation extract

After selecting the clean red ginseng, the red ginseng extract was obtained through hot water extraction, and sterilized by heat treatment at 120 ° C for 28 minutes. Then, Lactobacillus plantarum (Lactobacillus plantarum), a lactic acid bacteria extracted from kimchi, was inoculated and fermented at 27 ° C for 50 hours. The red ginseng fermented extract was first separated from the culture solution using an ultrasonic extractor, and then the supernatant obtained through centrifugation was collected to recover the red ginseng fermented extract second. The odor and color of the red ginseng ferment extract was removed by contacting the obtained red ginseng ferment extract with activated clay.

Preparation of fermented soybean extract

The soybeans were washed in running water, soaked in water for 12 hours, soaked, and sterilized by heat treatment at 120 ° C for 28 minutes. After that, Bacillus subtilis was inoculated into sterilized soybeans, and fermentation was performed at 36 ° C. for 50 hours. After soybean fermentation extract was firstly separated from the culture medium using an ultrasonic extractor, the soybean fermentation extract was secondarily separated by centrifuging the first separation solution to recover the supernatant. Thereafter, the separated soybean fermentation extract was contacted with activated clay to remove the smell and color of the soybean fermentation extract.

Preparation of rice ferment extract

The prepared rice was washed three times, soaked by immersion in water for 14 hours, and sterilized by heat treatment at 120 ° C for 28 minutes. Then, Saccharomyces cerevisiae was inoculated into sterilized rice and fermented at 27 ° C for 7 days. The red ginseng fermentation extract was firstly separated from the culture solution using an ultrasonic extractor, and the first fermentation solution was centrifuged to secondly separate the rice fermentation extract obtained by contacting with the active clay to remove the smell and color of the rice fermentation extract.

[Production Example 2]

Natural fermentation Cosmetic  Composition preparation

A fermentation stock solution was prepared by mixing 32 ml of red ginseng fermentation extract obtained in Preparation Example 1, 33 ml of soybean fermentation extract, and 35 ml of rice fermentation extract, and 65 ml of fermentation stock solution and 35 ml of 1,3-butylene glycol obtained by natural fermentation of sugar cane were mixed. .

Subsequently, a natural fermentation cosmetic composition was prepared by sequentially filtering and sterilizing a mixture of a fermentation stock solution and 1,3-butylene glycol in a filter of 1 μm, 0.5 μm, and 0.45 μm.

[Experimental Example 1]

The radical scavenging ability (DPPH) of the natural fermentation cosmetic composition prepared in Preparation Example 2 was measured using the Blois method.

First, 0.1 ml of a 0.15 mM DPPH (1,1-dyphenyl-2-picrylhydrazyl) solution was added to 0.1 ml of a natural fermented cosmetic composition mixed with purified water and prepared for each concentration, and allowed to stand in the dark at room temperature for 30 minutes, followed by absorbance at 518 nm. It was measured and the results are shown in Table 1. The DPPH radical scavenging ability described in Table 1 was compared with the control group without the natural fermentation cosmetic composition.

Item Example 1 Example 2 Example 3 Example 4 Concentration (mg / ml) 0.5 One 1.5 2 DPPH radical scavenging ability (%) 90.6 91.8 93.0 93.5

Looking at the experimental results in Table 1, when the natural fermentation cosmetic composition of the present invention is included, it can be seen that the radical scavenging activity is high, and the antioxidant ability is excellent. In addition, it can be seen that as the content of the natural fermentation cosmetic composition increases, the radical scavenging ability becomes higher.

Experimental Example 2

For 10 healthy adult men and women in their 20s and 30s, a skin product consisting of 3% by volume of the natural fermented cosmetic composition of the present invention and a residual amount of purified water was used 3 times a day for 4 weeks. Using the cutometer SEM 474, the skin elasticity of each subject was measured after 1 week, 2 weeks, 3 weeks, and 4 weeks of the experiment, and the average values are shown in Table 2. When measuring the skin elasticity, the subject was performed in a condition where the skin was stabilized in a constant temperature and humidity room with a temperature of 20 ° C and a relative humidity of 52% for 1 hour after washing, and the average value of the skin elasticity of each subject's eye was measured 5 times.

Item 1 week 2 weeks elapsed 3 weeks 4 weeks elapsed Skin elasticity (%) 10.34 12.26 14.78 16.51

Looking at the experimental results in Table 2, it can be seen that the skin elasticity is improved when the cosmetic product including the natural fermentation cosmetic composition of the present invention is steadily used.

The present invention is not limited to the specific embodiments and descriptions described above, and various modifications can be carried out by anyone having ordinary knowledge in the technical field to which the present invention pertains without departing from the gist of the present invention as claimed in the claims. And such modifications are within the protection scope of the present invention.

Claims (7)

In the natural fermentation cosmetic composition comprising a fermentation stock,
The fermentation stock solution, characterized in that it comprises a red ginseng fermentation extract, soybean fermentation extract and rice fermentation extract, natural fermentation cosmetic composition. According to claim 1,
The red ginseng fermentation extract is obtained by inoculating red ginseng extract with Lactobacillus plantarum, a natural fermentation cosmetic composition. According to claim 1,
The soybean fermentation extract is obtained by inoculating soybeans with Bacillus subtilis, a natural fermentation cosmetic composition. According to claim 1,
The rice fermentation extract is obtained by inoculating rice with Saccharomyces cerevisiae, a natural fermentation cosmetic composition. According to claim 1,
The natural fermentation cosmetic composition, characterized in that it contains 42-71% by volume of the fermentation stock solution and natural fermentation moisturizer 29-68% by volume, natural fermentation cosmetic composition. The method of claim 5,
The natural fermentation moisturizing agent, characterized in that obtained by fermenting sugar cane, natural fermentation cosmetic composition. After inoculating the red ginseng extract with Lactobacillus plantarum and fermenting, separating the red ginseng fermentation extract, a red ginseng fermentation extract extraction step;
Inoculating Bacillus subtilis to soybeans soaked in water and fermenting them, followed by separating the soybean fermentation extract, soybean fermentation extract extraction step;
Inoculating Saccharomyces cerevisiae on rice soaked in water and fermenting it, followed by separating the rice fermentation extract, extracting rice fermentation extract;
Fermentation stock solution manufacturing step of mixing the red ginseng fermentation extract, soybean fermentation extract and rice fermentation extract; And
Natural fermentation cosmetic composition manufacturing method comprising a; natural fermentation cosmetic composition manufacturing step of mixing the fermentation stock solution and a natural fermentation moisturizer.