KR101867308B1 - Cosmetic composition comprising Hydrangea macrophylla fermented extract for alleviating skin irritation and alleviating skin inflammation - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating skin irritation and alleviating skin inflammation, which comprises a hydrated fermented product as an active ingredient.
According to the present invention, the cosmetic composition containing the hydrated fermented product according to the present invention as an active ingredient inhibits the expression of inflammation-inducing cytokines (IL-6, IL-8, IL-1?) To reduce skin irritation and skin irritation Ultimately, it can be effectively applied to prevention and improvement of skin troubles, improvement of redness, prevention and improvement of dermatitis, and the like.

Description

Technical Field [0001] The present invention relates to a cosmetic composition for alleviating skin irritation and alleviating skin irritation containing hydrangea fermented product, and to a cosmetic composition for alleviating skin irritation and alleviating skin inflammation,

The present invention relates to a cosmetic composition for alleviating skin irritation, and more specifically, to a cosmetic composition for alleviating skin irritation and alleviating skin irritation containing a hydrated fermented product.

One of the most important functions of the skin is its protective function as a barrier. Skin, which is directly exposed to the external environment, is the most important primary line of defense against body fluids and protecting the body from harmful environments. It also acts as a barrier against toxic substances, microorganisms, physical stimuli or ultraviolet rays. However, when the skin barrier function receives various stimuli both inside and outside the skin, the skin barrier is damaged and the skin becomes dry or inflammation reaction occurs to the skin. In addition, failure to effectively control the initial inflammatory response at this time can lead to severe dermatitis.

However, due to the effects of population growth, urbanization, industrialization, and automobile growth, air pollution is intensifying and the types of pollutants are becoming diverse. Especially, due to the local factors of Korea, it is faced with damages such as dust and fine dust due to the movement of air pollutants emitted from China, and its seriousness is also increasing. Particulate matter is classified into fine dust (PM10) with a diameter of 10 ㎛ or less and ultra fine dust (PM2.5) with a diameter of 2.5 ㎛ or less depending on the particle size. Korea's Ministry of Environment regulates PM10 as an air pollutant. Air pollutants, such as fine dust, are known to cause respiratory and heart diseases, and their effects on the skin, which occupies a large area in the body, are also increasingly being gained. Especially, it is reported that fine dust among air pollutants damages epidermal barrier function and worsens atopic dermatitis. In addition, sensitive skin responds to external stimuli or environmental changes more sensitively than normal skin, resulting in irritation or dermatitis symptoms. Those who have sensitive skin have more than three times more irritation than those who have normal skin. Therefore, pollutants such as fine dusts are likely to cause skin problems, especially for people with sensitive skin. This also affects skin aging, but recent epidemiological studies have shown that skin exposed to fine dust increases wrinkles and pigmentation spots and is directly related to aging. Fine dusts produce active oxygen, which can inhibit collagen synthesis and activate degradation. Polycyclic aromatic hydrocarbons (PAH) adsorbed on the fine dust surface activate the metabolism of the living body through the AhR to increase collagen degradation. do. Therefore, a new treatment method should be sought by studying the ways of reducing direct contact between fine dust and skin and studying molecular mechanisms.

The inflammatory response in the skin begins as an action to defend skin damage caused by physical stimuli, chemicals, and bacteria, and involves a variety of immune cells and inflammation-inducing cytokines. IL-6 (interleukin-6), IL-8 (interleukin-8) and IL-1β (interleukin-β) are typical inflammation-inducing cytokines. IL-1β is a typical cytokine that is produced by activated mononuclear cells, epithelial cells, and vascular endothelial cells and mediates the inflammatory response. IL-6 stimulates the immune response by secretion by T cells and macrophages, It is a cytokine that regulates. Activated macrophages also activate inflammatory processes by overexpressing nitric oxide (NO) or prostagladin E2 (PGE2) as well as inflammation-inducing cytokines. It has been reported that accelerated and anti-inflammatory microparticles increase the expression of these inflammatory cytokines in human keratinocytes.

Conventionally, various attempts have been made to develop cosmetics capable of imparting anti-inflammatory function to skin using natural extracts. As a typical example thereof, Korean Patent Publication No. 2007-0011939 proposes a cosmetic composition containing camphor flower extract Korean Patent Publication No. 2010-0086698 proposes a cosmetic composition for skin soothing and irritation mitigation comprising an extract of mung bean fermentation-enzyme, Korean Patent Laid-Open Publication No. 2013-0016930 discloses a cosmetic composition for sily, lemon balm, mugwort and onion extract As an active ingredient, to cosmetic compositions for alleviating atopic skin and skin irritation. Korean Patent Laid-Open Publication No. 2015-0078948 proposes a cosmetic composition for improving skin irritation or alleviating skin irritation, which comprises a mixed extract of mistletoe, pine bark and gossypol as an active ingredient. In Korean Patent Publication No. 2009-0091547, A cosmetic composition for antiinflammation and skin irritation mitigation containing a mixed extract of casualties is proposed. However, none of the above-mentioned cosmetic compositions has the effect of alleviating skin irritation and alleviating skin irritation of the hydrated fermented extract of the present invention like the present invention.

As a result of efforts to overcome the problems of the prior arts, the inventors of the present invention have found that a substance which recognizes that inflammatory cytokines increased by various stimuli, especially fine dust stimuli, are related to skin irritation and skin irritation, The extracts of the plant extracts inhibiting the expression of inflammatory cytokines were screened and screened for their ability to inhibit the expression of IL-6, IL-8 and IL-1β, the inflammation-inducing cytokines, in fermented fermented water extracts. Thereby confirming the efficacy of alleviating skin irritation and skin inflammation, thereby completing the present invention.

KR 10-2007-0011939 A KR 10-2010-0086698 A KR 10-2013-0016930 A KR 10-2015-0078948 A KR 10-2009-0091547 A

Accordingly, it is a primary object of the present invention to provide a medicament for relieving skin irritation and skin irritation by inhibiting the expression of IL-6, IL-8 and IL-l [beta], which are inflammation-inducing cytokines increased by various stimuli, Which comprises the hydrated fermented product of the present invention.

Still another object of the present invention is to provide a hydrate fermented product of the present invention as a base formulation of a functional cosmetic composition to prevent skin irritation and alleviate skin irritation, thereby preventing and improving skin troubles, And to provide a cosmetic composition which can be applied.

According to one aspect of the present invention, there is provided a cosmetic composition for alleviating skin irritation and alleviating skin inflammation, which comprises a hydrated fermented product as an active ingredient.

Hydrangea ( Hydrangea macrophylla ) is a deciduous shrub of rosewood bumblebees. Flowers bloom in June ~ July, and the light purple color at first becomes light blue, then becomes light red again. Depending on the soil, the flower color changes to white if it is neutral, blue if it is acidic, or pink if it is alkaline. Leafy oak leaves are oval-shaped, with opposite saw-toothed edges, 10 to 15 cm long, and sharp edges. Flowers are bloomed in early summer and are known more as garden plants, but their efficacy is known as medicinal herbs and antipyretics for treating heart diseases. It uses mainly outposts containing roots. A whitening effect (Korean Patent Laid-Open Publication No. 2014-0082555), and an effect of improving the skin aging phenomenon (Korean Patent Publication No. 2009-0081178). However, as described in the present invention, the effect of improving the skin irritation against the external stimulation of the hydrangea fermented extract through the divalent fermentation is not mentioned. Accordingly, the inventors of the present invention confirmed that the hydrangea fermented product can alleviate skin irritation and skin inflammation by inhibiting the expression of inflammation-inducing cytokines, and completed the present invention.

In the present invention, the hydrangea fermented product is characterized in that the hydrangea extract is fermented by using a dicer.

Specifically, the mixture is mixed at a ratio of 10% of rice cakes, 1% of hydrangea extract, and the remaining weight of 89% in water, and the mixture is stirred at 25 ° C, And then cultivated for 4 days.

In the present invention, the rice bran is rice koji, preferably rice koji rice, which is prepared by inoculating a koji culture with a yeast liquid culture.

Specifically, the koji culture is a solid culture (Koji) obtained by sterilizing rice modified at 38 ° C to 42% moisture at 121 ° C for 30 minutes, cultivating and drying inoculated with Aspergillus oryzae. Also, a yeast liquid culture (Saccharomyces cerevisiae) refers to a culture in which the yeast is cultured in a potato dextrose liquid medium.

In the present invention, the hydrangea extract may be extracted with any solvent conventionally used, and is preferably extracted with water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin or a mixed solvent thereof .

Wherein the hydrangea extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin or a mixed solvent thereof.

As the extraction method using the above-mentioned extraction solvent, conventional methods of extracting plants such as hot water extraction, reflux cooling extraction, ultrasonic extraction and supercritical extraction can be used. Specifically, hydrangea is crushed to make powder , Immersed in the aforementioned extraction solvent, and extracted at room temperature for 24 to 72 hours.

In the present invention, the hydrated fermented product is characterized by containing 0.001 to 30% by weight based on the total weight of the cosmetic composition. If the amount of the hydrangea fermented product is less than 0.001% by weight, the effect of alleviating the skin irritation may be insufficient. On the contrary, if the amount is more than 30% by weight, it may not be preferable from the economical point of view.

In the present invention, the hydrated fermented product is characterized by alleviating skin inflammation by inhibiting inflammatory cytokines.

According to the experimental example of the present invention, the inhibitory effect of the inflammatory cytokine increased due to the fine dust was confirmed. As a result, the hydrangea fermented product of the present invention showed an excellent inhibitory effect. In particular, More excellent. From these results, it can be seen that the hydrated fermented product of the present invention not only alleviates skin irritation by inhibiting inflammation-inducing cytokines but also alleviates skin irritation such as fine dust.

As a result of confirming the effect of alleviating the stimulation of the skin layer by the fine dust, it was confirmed that the skin surface layer and the epidermal cell damaged by the stimulation were recovered in the case of the hydrated fermented product of the present invention. From these results, it can be seen that the hydrangea fermented product can alleviate the damage of the skin layer due to the stimulation by the fine dust.

In the present invention, the cosmetic composition may be at least one selected from the group consisting of a soft lotion, a nutritional lotion, a nutritional essence, a nutrient oil, a moisturizing oil, a nutritive cream, a powder, a pack, a foundation, a makeup base, a cleansing, a shampoo, And is a formulation.

 The ingredients contained in the cosmetic composition of the present invention may contain, as an active ingredient, the ingredients conventionally used in cosmetic compositions in addition to the above-mentioned fermented product, for example, conventional additives such as stabilizers, solubilizers, vitamins, And a carrier.

As described above, the hydrated fermented product of the present invention effectively inhibits the expression of inflammation-inducing cytokines (IL-6, IL-8, IL-1?) Overexpressed by various stimuli, particularly fine dusts, It can relieve inflammation.

1 is a graph showing the results of Experimental Example 1 showing the degree of inhibition of IL-6 gene expression among inflammation-inducing cytokines.
2 is a graph showing the results of Experimental Example 1 showing the degree of inhibition of IL-8 gene expression among inflammation-inducing cytokines.
3 is a graph showing the results of Experimental Example 1 showing the degree of inhibition of IL-1? Gene expression among inflammation-inducing cytokines.
FIG. 4 is a photograph showing the result of measuring the recovery of the skin layer damaged by the fine dust when the hydrocortisone fermented extract is treated.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example  1: Preparation of hydrangea extract

The hydrangea was pulverized, and each 100 g was taken. Each of the extracts was placed in a separate vessel, immersed in 1 L of 70 wt% ethanol aqueous solution as an extraction solvent, and left at room temperature for 24 hours to obtain an extract. The obtained extract was filtered using a filter paper, and the filtrate was concentrated under reduced pressure using a rotary evaporator to obtain an extract.

Example  2: Hydrangea Fermented  Produce

The hydrangea extract obtained in Example 1 was prepared by using dicot. It is characterized by using two kinds of koji culture and yeast liquid culture.

The koji culture is a solid culture (Koji) cultured and dried by sterilization of rice adjusted at 38 ~ 42% moisture at 121 ℃ for 30 min, inoculated with Aspergillus oryzae. A yeast liquid culture (Saccharomyces cerevisiae) refers to a culture in which the yeast is cultured in a potato dextrose liquid medium.

The rice cake of the present invention is prepared by the following method. First, 100 parts by weight of washed rice is dried at 60 DEG C for 20 to 24 hours and then pulverized. The powder having passed through a sieve of about 40 mesh is recovered so as to have a moisture content of 40 to 45% and then formed into a round shape. The rounded rice is formed again with a hole-shaped rice cake, and then the mixture is heated at 100 ° C for 1 hour. Two types of pre-cultured koji culture and yeast liquid culture are inoculated to be 1% of each weight part. It is cultured at 25 ℃ for 4 days to complete the song.

The hydrangea fermented product was prepared by mixing 10% of Rice hull, 1% of Hydrangea extract, and 89% of water in the remaining part by weight and anaerobically culturing at 25 캜 for 4 days, and used in Experimental Example 1 below.

Experimental Example  1: Evaluation of anti-inflammatory efficacy

In order to evaluate the anti-inflammatory effects of the fermented extracts, the expression levels of IL-1, IL-6, IL-8, PM10 were measured in the inflammation-inducing cytokines. More specifically, HaCaT cells, which are keratinocytes, were treated with fine dusts, and the respective raw materials prepared in Examples 1 and 2 were cultured for 4 hours to a final concentration of 10 占 퐂 / ml. Then, mRNA was extracted from the cells, synthesized as cDNA, real-time PCR was performed using a target primer, and the degree of gene expression of the inflammatory cytokine was finally evaluated. The results are shown in Figs.

     As a result, as shown in Figs. 1 to 3, when the respective cytokines were induced by the fine dust, the degree of inhibition of the hydrangea extract and the hydrangea fermentation extract was evaluated. Showed a remarkable induction inhibitory effect. Therefore, it was confirmed that the fermented extract according to the present invention is a very useful raw material for a cosmetic composition having a higher anti-inflammatory effect than before fermentation.

Experimental Example  2: Evaluation of the effect of skin stimulation relaxation

In order to evaluate the effect of mitigation of skin epidermal irritation, artificial skin tissue cultured in three dimensions was used to evaluate the mitigation effect of the skin layer stimulated and damaged by fine dust. 50 占 퐂 / ml of dust fine dust (PM10) was applied to the artificial skin surface, and the hydrangea extract and hydrangea diphtheria fermentation extract obtained in Examples 1 and 2 were treated at a concentration of 10 占 퐂 / ml in the medium. After incubation for 24 hours, the cells were fixed in formalin and paraffin blocks were prepared and stained with H & E. The morphology of the stratum corneum and keratinocytes was measured. The results of the measurement are shown in Fig.

As a result, as shown in FIG. 4, it was confirmed that the stratum corneum of the artificial skin tissue damaged by the fine dust treatment was alleviated. It was also confirmed that the density and shape of keratinocytes in the basal layer were restored. Therefore, it was confirmed that the fermented extract according to the present invention is a very useful raw material for cosmetic compositions having a stimulating effect on the skin layer damaged by stimulation.

Formulation Example  1: Lotion production

The lotion containing the fermented extract obtained in Example 2 was prepared by a conventional method according to the composition components and the composition ratios shown in Table 1 below.

ingredient Content (% by weight) Hydrangea fermentation extract 1.0 glycerin 5.0 ethanol 3.0 Polyoxyethylene hardened castor oil 0.5 Polysorbate bit 80 0.5 Butylene glycol 3.0 Carboxyl vinyl polymer 0.5 Preservative, fragrance Suitable amount Purified water Balance

Formulation Example  2: Manufacture of nutritional essence

The moisture cream containing the fermented extract obtained in Example 2 was prepared by a conventional method according to the composition components and composition ratios shown in Table 2 below.

ingredient Content (% by weight) Hydrangea fermentation extract 1.0 glycerin 7.0 Butylene glycol 3.0 Glyceryl stearate / phage-100 suearate 1.0 Polysorbate 60 0.5 Sorbitan stearate 0.5 Squalane 3.0 Caprylic / capric triglyceride 5.0 Shea butter 2.0 Stearyl alcohol 0.5 Sutearin acid 0.5 Carboxyl vinyl polymer 0.2 Arginine 0.2 Preservative, fragrance Suitable amount Purified water Balance

Formulation Example  3: Moisturizing Cream

A moisturizing cream containing the fermented extract obtained in Example 2 was prepared by a conventional method according to the composition components and composition ratios shown in Table 3 below.

ingredient Content (% by weight) Hydrangea fermentation extract 1.0 glycerin 7.0 Butylene glycol 5.0 Glyceryl stearate / phage-100 suearate 2.0 Polysorbate 60 0.5 Sorbitan stearate 0.5 Phitosqualan 5.0 Jojoba oil 3.0 Caprylic / capric triglyceride 10.0 Aloe butter 3.0 Wax 1.0 Stearyl alcohol 0.5 Beeryl alcohol 0.5 Stearic acid 0.5 Carboxyl vinyl polymer 0.2 Xanthan gum 0.2 Arginine 0.3 Dimethicone 0.5 Beta Glucan 0.2 Preservative, fragrance Suitable amount Purified water Balance

As described above, according to the present invention, the cosmetic composition containing the hydrated fermented product according to the present invention as an active ingredient inhibits the expression of inflammation-inducing cytokines (IL-6, IL-8, IL-1β) By showing skin inflammation relief, ultimately, it can be effectively applied to prevention and improvement of skin trouble, improvement of redness, prevention and improvement of dermatitis, and the like.

Claims (8)

A cosmetic composition for alleviating skin irritation and alleviating skin irritation, which comprises a hydrangea fermented product obtained by fermenting hydrangea extract with a dicer.
delete The cosmetic composition for relieving skin irritation and alleviating skin irritation according to claim 1, wherein the dichotoma is prepared using a co-culture product and a yeast liquid culture product.
The cosmetic composition according to claim 1, wherein the hydrangea extract is extracted with water, a lower alcohol having 1 to 4 carbon atoms, butylene glycol, propylene glycol, glycerin or a mixed solvent thereof. .
[2] The cosmetic composition for relieving skin irritation and alleviating skin irritation according to claim 1, wherein the hydrated fermented product is contained in an amount of 0.001 to 30% by weight based on the total weight of the cosmetic composition.
The cosmetic composition for skin irritation mitigation and skin irritation according to claim 1, wherein the hydrated fermented water alleviates skin inflammation by inhibiting the expression of inflammatory cytokines.
The cosmetic composition for alleviating skin irritation and alleviating skin irritation according to claim 1, wherein the hydrated fermented water alleviates inflammation of the skin by inhibiting inflammatory cytokines.
The cosmetic composition according to claim 1, wherein the cosmetic composition is selected from the group consisting of a soft lotion, a nutritional lotion, a nutritional essence, a nutritional oil, a moisturizing oil, a nutritional cream, a powder, a pack, a foundation, a makeup base, a cleansing, a shampoo, Or less of the total amount of the cosmetic composition.

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