KR102184985B1 - Functional cosmetic composition comprising hydrogen and phytoncide as active ingredients - Google Patents

Abstract

A functional cosmetic composition comprising hydrogen and phytoncide as active ingredients according to the present invention inhibits the production of NO overexpressed by fine dust and effectively inhibits the production of inflammatory cytokines IL-6, IL-8, IL-1beta, and TNF-alpha, and thus can be used as a cosmetic composition for preventing or alleviating skin inflammation. Here, the when together with the hydrogen and phytoncide, a natural material such as a blueberry extract, a black rice extract, a licorice extract, a black garlic extract, or a turmeric extract is further included, the production of NO overexpressed by fine dust is inhibited. It is confirmed that the production of inflammatory cytokines IL-6, IL-8, IL-1beta, and TNF-alpha can be more effectively inhibited. Thus, the composition of the present invention can be used as a cosmetic composition for preventing or alleviating skin inflammation.

Description

Functional cosmetic composition comprising hydrogen and phytoncide as active ingredients}

The present invention relates to a functional cosmetic composition comprising hydrogen and phytoncide as active ingredients, and more specifically to a functional cosmetic composition comprising a high concentration of hydrogen and phytoncide as active ingredients, and further comprising a complex natural extract.

The skin is a part of the body that is directly exposed to the external environment, and not only acts as a protective film to protect important organs of our body, but also controls water evaporation and protects the body from external infections. However, no matter how much the skin prevents the penetration of viruses from the outside, stress received from the outside, such as excessive ultraviolet rays and a contaminated environment, causes skin irritation and eventually leads to skin aging.

However, due to the influence of population growth, urbanization, industrialization, and automobile growth, air pollution is intensifying, and accordingly, the types of pollutants are diversifying. Particularly, as a locational factor in Korea, it faces damage such as yellow dust and fine dust due to the movement of air pollutants discharged from China, and its severity is increasing. Particulate matter is classified into fine dust with a diameter of 10㎛ or less (PM ₁₀ ) and ultrafine dust with a diameter of 2.5㎛ or less (PM _2.5 ) depending on the size of the particle. Korea's Ministry of Environment regulates PM ₁₀ as an air pollutant. Air pollutants such as fine dust are known to cause respiratory disease and heart disease, and at the same time, the effect on the skin, which occupies a large area of the body, is gradually expanding its interest.

In particular, it is reported that fine dust among air pollutants impairs the epidermal barrier function and worsens atopic dermatitis. In addition, sensitive skin reacts more sensitively to external stimuli or changes in the environment than general skin and shows irritation or dermatitis symptoms. There was a study in which people with such sensitive skin felt more than three times the sensation of irritation because they were more sensitive to the degree of stimulus to fine dust than normal people. Therefore, pollutants such as fine dust are highly likely to cause skin problems, especially in people with sensitive skin. This also affects skin aging, and a recent epidemiologic study revealed that skin exposed to fine dust has increased wrinkles and pigment spots, which is directly related to aging. Fine dust can inhibit collagen synthesis and activate decomposition by producing active oxygen, and PAH (polycyclic aromatic hydrocarbon) adsorbed on the surface of fine dust activates the metabolism of biological foreign substances through AhR, increasing collagen degradation, causing skin aging. do. Accordingly, it is necessary to seek new treatments through research on molecular mechanisms and methods to reduce direct contact between fine dust and skin.

(interleukin-1

) 등이 대표적인 염증 유도 사이토카인이다. IL-1

는 활성화된 단핵식균세포, 상피세포, 혈관내피세포에 의해 만들어져 염증 반응을 매개하는 전형적인 사이토카인이고, IL-6는 T세포 및 대식세포에 의해 분비되어 면역 반응을 자극하며 염증 반응을 조절하는 사이토카인이다. 또한 활성화된 대식세포 (macrophage)는 염증성 사이토카인뿐만 아니라 일산화질소 (nitric oxide: NO)나 프로스타글란딘 (prostagladin) E2 (PGE2)를 과도하게 생성하여 염증 과정을 더욱 활성화시킨다. 미세먼지 입자는 사람 각질형성세포에서 이러한 염증성 사이토카인의 발현을 증가시킨다는 보고가 있다.The inflammatory response in the skin begins as an action to protect skin damage when it is caused by physical stimulation, chemical substances, bacteria, etc., and various immune cells and inflammatory cytokines are involved. IL-6 (interleukin-6), IL-8 (interleukin-8), IL-1

(interleukin-1

), etc. are representative cytokines that induce inflammation. IL-1

Is a typical cytokine produced by activated mononuclear phagocytes, epithelial cells, and vascular endothelial cells to mediate the inflammatory response, and IL-6 is secreted by T cells and macrophages to stimulate the immune response and regulate the inflammatory response. It's Cain. In addition, activated macrophages produce inflammatory cytokines as well as nitric oxide (NO) or prostaglandin E2 (PGE2) excessively to further activate the inflammatory process. It has been reported that fine dust particles increase the expression of these inflammatory cytokines in human keratinocytes.

Accordingly, the present inventors have completed the present invention by confirming the efficacy of alleviating skin inflammation caused by fine dust, including high concentration of hydrogen and phytoncide as active ingredients, and further including some natural extracts, which can be used as functional cosmetics. There will be.

Korean Patent Registration No. 10-1867308

It is an object of the present invention to provide a functional cosmetic composition that can alleviate skin inflammation caused by fine dust by including hydrogen and phytoncide as active ingredients.

Another object of the present invention is to provide a functional cosmetic composition comprising a high concentration of hydrogen and phytoncide as active ingredients, and further comprising a natural extract to more effectively alleviate skin inflammation caused by fine dust.

To solve the above object,

The present invention provides a functional cosmetic composition comprising hydrogen and phytoncide as active ingredients.

In addition, the present invention provides a functional cosmetic composition comprising a high concentration of hydrogen and phytoncide as active ingredients, and further comprising a natural extract.

At this time, the natural extract is blueberry extract, black rice extract, licorice extract, black garlic extract or turmeric extract, and provides a functional cosmetic composition that can more effectively alleviate skin inflammation caused by fine dust.

및 TNF-

의 생성을 효과적으로 억제하여 피부 염증 예방 또는 개선용 화장료 조성물로 사용될 수 있다. 여기에서, 상기 수소 및 피톤치드에 천연 추출물은 블루베리 추출물, 흑미 추출물, 감초 추출물, 흑마늘 추출물 또는 울금 추출물을 더 포함하는 경우 미세먼지에 의해 과발현된 NO 생성을 억제하고, 염증성 사이토카인인 IL-6, IL-8, IL-1

및 TNF-

의 생성을 더욱 효과적으로 억제함을 확인하여 피부 염증 예방 또는 개선용 화장료 조성물로 사용될 수 있다.Functional cosmetic composition comprising hydrogen and phytoncide according to the present invention as active ingredients inhibit the generation of NO overexpressed by fine dust, and inflammatory cytokines IL-6, IL-8, IL-1

And TNF-

It can be used as a cosmetic composition for preventing or improving skin inflammation by effectively inhibiting the production of. Here, when the natural extract to the hydrogen and phytoncide further includes blueberry extract, black rice extract, licorice extract, black garlic extract or turmeric extract, it inhibits NO generation overexpressed by fine dust, and IL-6, an inflammatory cytokine , IL-8, IL-1

And TNF-

It can be used as a cosmetic composition for preventing or improving skin inflammation by confirming that it more effectively inhibits the production of.

생성 억제를 확인한 결과이다.
도 7은 본 발명에 따른 실시예 1의 피톤치드 및 복합 천연 추출물에 수소를 첨가한 추출물의 TNF-

생성 억제를 확인한 결과이다.1 is a result of confirming (a) cytotoxicity and (b) NO production amount in RAW 264.7 cells according to fine dust treatment by concentration.
2 is a result of confirming the cytotoxicity of the extract obtained by adding hydrogen to the phytoncide and complex natural extract of Example 1 according to the present invention.
3 is a result of confirming the suppression of NO generation of the extract obtained by adding hydrogen to the phytoncide and complex natural extract of Example 1 according to the present invention.
4 is a result of confirming the inhibition of IL-6 production of the extract obtained by adding hydrogen to the phytoncide and complex natural extract of Example 1 according to the present invention.
5 is a result of confirming the inhibition of IL-8 production of the extract obtained by adding hydrogen to the phytoncide and complex natural extract of Example 1 according to the present invention.
6 is IL-1 of the extract obtained by adding hydrogen to the phytoncide and complex natural extract of Example 1 according to the present invention.

This is the result of confirming the suppression of production.
7 is a TNF- of the extract obtained by adding hydrogen to the phytoncide and complex natural extract of Example 1 according to the present invention

This is the result of confirming the suppression of production.

Hereinafter, the present invention will be described in detail.

About the functional cosmetic composition for preventing or improving skin inflammation caused by fine dust of the present invention,

The present invention provides a cosmetic composition for preventing or improving skin inflammation caused by fine dust comprising an extract obtained by adding hydrogen to complex natural extracts.

In the cosmetic composition of the present invention, the extract obtained by adding hydrogen to the complex natural extracts

Preparing a complex natural extract (blueberry, black rice, licorice, black garlic, or turmeric) (step 1);

Adding cypress phytoncide to the complex natural extract prepared in step 1 (step 2); And

It may be prepared by a manufacturing method including the step of injecting a high concentration of hydrogen into the complex natural extract to which the cypress phytoncide prepared in step 2 is added (step 3), but is not limited thereto.

In one embodiment of the present invention, the extract may be extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof to prepare an extract, preferably extracted using a 30 to 50% ethanol aqueous solution and then concentrated. However, the extract can be prepared, but is not limited thereto.

The term "extract" as used in the present invention refers to a preparation obtained by squeezing the object of extraction into an appropriate leach solution and evaporating the leachate, but is not limited thereto, but is not limited thereto, an extract obtained by an extraction treatment, a diluted solution or a concentrate of the extract , It may be a dried product obtained by drying the extract, a preparation thereof, or a purified product. The extract obtained by adding hydrogen to the complex natural extracts may be prepared using a general extraction method, separation and purification method known in the art. As the extraction method, although not limited thereto, a method such as hot water extraction, hot water extraction, cold needle extraction, reflux cooling extraction, or ultrasonic extraction may be used.

The cosmetic composition of the present invention can be prepared including a cosmetically effective amount of an extract obtained by adding hydrogen to the composite natural extracts of the present invention and a cosmetically acceptable carrier.

The cosmetic composition contains a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, such as solutions, gels, solids, dough anhydrous products, emulsions obtained by dispersing the oil phase in an aqueous phase, suspensions, microemulsions, microcapsules, microgranules or, ionic (liposomes) and It may be provided in the form of a nonionic vesicle dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. These compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

The cosmetic composition according to an embodiment of the present invention is not particularly limited in its formulation, for example, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream , Nutritional cream, moisture cream, hand cream, foundation, essence, nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

When the formulation of the present invention is a paste, cream, or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as carrier components. I can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linoline derivatives, or ethoxylated glycerol fatty acid esters may be used.

The cosmetic composition according to an embodiment of the present invention may further include functional additives and components included in general cosmetic compositions in addition to the extract obtained by adding hydrogen to the complex natural extracts. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingo lipids, and seaweed extract.

In addition to the above functional additives, the cosmetic composition of the present invention may further contain ingredients contained in a general cosmetic composition, if necessary. Other ingredients included include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation Examples include accelerators, cooling agents, restrictors, and purified water.

The present invention will be described in more detail through the following examples. However, the following examples are only for embodiing the contents of the present invention and the present invention is not limited thereto.

<Example 1> Preparation of hydrogen, phytoncide and natural extracts (blueberry, black rice, licorice, black garlic and turmeric)

<1-1> High concentration hydrogen production (dissolved hydrogen measurement according to ultrasonic generation and degassing conditions)

Dissolved hydrogen was measured according to the conditions of ultrasonic treatment and ultrasonic + degassing using a process for producing high-concentration hydrogen. No degassing or ultrasonic treatment was used as a control, and ultrasonic waves were generated at a frequency of 40 kHz, and vacuum pressure was performed at a pressure of about 0.5 bar. Thereafter, the dissolved oxygen in the cosmetic composition was measured after injecting hydrogen gas for 3 minutes. The results of comparing each dissolved hydrogen are shown in Table 1.

Processing time (minutes) Control (ppm) Ultrasound (ppm) Ultrasonic + degassing (ppm) 1 min 0.65 0.78 0.98 3 minutes 0.65 0.79 1.00 5 minutes 0.65 0.81 1.15 10 minutes 0.65 0.80 1.08

Dissolved hydrogen in the control group without degassing or ultrasonic treatment was 0.65 ppm, and the dissolved hydrogen in the experimental group treated with only ultrasound without degassing treatment was 0.78 to 0.82 ppm, and no effective dissolved hydrogen injection occurred compared to the control dissolved hydrogen. In contrast, when the ultrasonic and degassing treatment were performed together, the dissolved hydrogen level was 0.98 to 1.15 ppm, and it was found that hydrogen was efficiently injected only by ultrasonic and vacuum degassing without introducing nitrogen gas, especially in a time period of 5 minutes. In one case, it was confirmed that hydrogen was most effectively injected.

<1-2> Preparation of cypress phytoncide

Cypress phytoncide was purchased and used from phytoncide specialty mall, Forest Miracle (www.kijuk.co.kr), and the weight ratio (w/w) of Cypress phytoncide in the same amount as the following complex natural extract was added.

<1-3> Preparation of blueberry extract

Blueberry (US) 30 times (wt/v%) of 50% ethanol was added to extract for 24 hours at room temperature, and the extraction process was repeated three times. The blueberry extract was concentrated and concentrated to a final sugar content of 50 to 70° Brix. After the concentrate was filtered, it was diluted 1/10 with water.

<1-4> Preparation of black rice extract

30 times (wt/v%) of 50% ethanol was added to the weight of black rice (domestic), followed by extraction at room temperature for 24 hours, and the extraction process was repeated three times. The black rice extract was concentrated and concentrated to a final sugar content of 10 to 30° Brix. After the concentrate was filtered, it was diluted 1/5 with water.

<1-5> Preparation of licorice extract

By adding 30 times (wt/v%) of 50% ethanol to the weight of licorice (domestic), extraction was performed at room temperature for 24 hours, and the extraction process was repeated three times. The licorice extract was concentrated and concentrated to a final sugar content of 10 to 30° Brix. After the concentrate was filtered, it was diluted 1/5 with water.

<1-6> Preparation of black garlic extract

50% ethanol was added 30 times (wt/v%) to the weight of black garlic (domestic) and extracted for 24 hours at room temperature, and the extraction process was repeated three times. The black garlic extract was concentrated and concentrated to a final sugar content of 10 to 30° Brix. After the concentrate was filtered, it was diluted 1/5 with water.

<1-7> Preparation of turmeric extract

The extraction was performed at room temperature for 24 hours by adding 30 times (wt/v%) of 50% ethanol to the weight of turmeric (domestic), and the extraction process was repeated three times. The turmeric extract was concentrated and concentrated to a final sugar content of 10 to 30° Brix. After the concentrate was filtered, it was diluted 1/5 with water.

<1-8> Preparation of cosmetic composition containing hydrogen, phytoncide and natural extract

In the mixing of Examples 1-1 to 1-7, the step of purifying water using a carbon filter (T1), preparing a cosmetic composition of a natural complex extract containing phytoncide (T2), and hydrogen gas A final cosmetic composition may be obtained by performing a process including the step of injecting (T3). In the composition of the present disclosure, the content of hydrogen is preferably 0.9 to 1.2 ppm based on the total weight of the cosmetic composition for moisturizing and improving skin. When the above hydrogen is added to a cosmetic composition containing a natural substance, the active hydrogen removes active oxygen from the skin when applied to the skin and prevents skin aging by preventing cell death due to cell aging. Accordingly, the user's skin tone is cleared, spots and freckles are improved or disappeared, and the effect of promoting skin generation and preventing aging can be obtained.

<Comparative Example 1>

It was prepared in the same manner as in Example 1, except that cypress phytoncide was not mixed, and hydrogen was added to blueberry, black rice, licorice, black garlic, and turmeric extracts, and used as Comparative Example 1.

<Comparative Example 2>

Prepared in the same manner as in Example 1, but without mixing blueberry, black rice, licorice, black garlic and turmeric extracts, hydrogen was added to Cypress phytoncide and used as Comparative Example 2.

<Example 2> Cell culture

5% CO₂ 조건하에 10% FBS (fetal bovine serum) 및 1% 페니실린-스트렙토마이신 (PS)을 포함하는 고글루코스 DMEM (WELGENE, KOREA) 배양액에서 배양하였다. Keratinocytes (Human Keratinocyte Cell Lines, HaCaT) were purchased from Korea Cell Line Bank (Seoul National University, Seoul, Korea). HacaT cells 37

It was cultured in a high glucose DMEM (WELGENE, KOREA) culture medium containing 10% Fetal bovine serum (FBS) and 1% penicillin-streptomycin (PS) under 5% CO ₂ conditions.

<Experimental Example 1> Confirmation of fine dust treatment conditions

To set the optimal concentration representing the amount of NO generation at a level similar to LPS (1 μg/ml), the fine dust in the HaCaT cells of Example 2 was 0.1, 1, 10, 25, 50, 100, 250 and 500 μg/ml. After treatment, cytotoxicity and NO production were measured.

As a result, as shown in FIG. 1, it was confirmed that when fine dust was treated at concentrations of 250 and 500 µg/ml, NO generation similar to LPS was observed. Therefore, in the subsequent experiment, the experiment was performed by treating the fine dust at a concentration of 250 μg/ml.

<Experimental Example 2> Cytotoxicity evaluation

5% CO₂ incubator에서 24시간 배양한 후 실시예 1의 피톤치드 및 천연 추출물들에 수소를 첨가한 추출물을 100, 200 및 500㎍/㎖의 농도로 처리하고 이에 미세먼지를 250㎍/㎖로 처리하여 24시간동안 배양하였다. 이후 0.2 ㎎/㎖ 농도의 MTT solution을 각 well에 100㎕씩 넣고 2시간 동안 배양한 후 상층액을 제거하고 DMSO를 100㎕씩 분주하여 formazan을 잘 녹인 후 570nm에서 흡광도를 측정하였다. Dispense HaCaT cells into a 96-well plate (Corning, USA) to a cell number of 2Х10 ⁴ cells/well by dispensing 37

After culturing in a 5% CO ₂ incubator for 24 hours, the phytoncide of Example 1 and the extract obtained by adding hydrogen to the natural extracts were treated at concentrations of 100, 200 and 500 µg/ml, and fine dust was treated with 250 µg/ml. And incubated for 24 hours. Thereafter, 100 µl of 0.2 mg/ml MTT solution was added to each well and incubated for 2 hours. The supernatant was removed, and 100 µl of DMSO was dispensed to dissolve formazan well, and the absorbance was measured at 570 nm.

As a result, as shown in FIG. 2, it was confirmed that the extract obtained by adding hydrogen to phytoncide and natural extracts did not exhibit cytotoxicity.

<Experimental Example 3> Confirmation of NO generation inhibition

5% CO₂ incubator에서 24시간 배양한 후, 미세먼지를 250㎍/㎖농도로 처리하고, 비교예 1, 2 및 실시예 1을 각각 200㎍/㎖ 농도로 처리하여 18시간 동안 배양하였다. 이후 상등액 100㎕와 Griess reagent 100㎕을 혼합하여 상온에서 10분간 반응시킨 뒤, 540nm에서 흡광도를 측정하였다. Nitrite standard로 표준곡선을 작성하여 NO의 생성량을 측정하였다. In order to confirm the suppression of NO production of the extract obtained by adding hydrogen to the phytoncide and natural extracts of Example 1 according to the present invention, HaCaT cells were dispensed into a ⁶ well plate (Corning, USA) to a cell number of 1 Х10 ⁶ cells/well. 37

After culturing in a 5% CO ₂ incubator for 24 hours, fine dust was treated at a concentration of 250 µg/ml, and Comparative Examples 1, 2 and Example 1 were treated at a concentration of 200 µg/ml, respectively, and cultured for 18 hours. Thereafter, 100 µl of the supernatant and 100 µl of Griess reagent were mixed and reacted at room temperature for 10 minutes, and the absorbance was measured at 540 nm. A standard curve was created with the nitrite standard to measure the amount of NO produced.

As a result, as shown in Figure 3, when treating the extract obtained by adding hydrogen to the phytoncide and natural extracts of Example 1 of the present invention compared to Comparative Examples 1 to 2, NO generation induced by fine dust was reduced. It was confirmed that it was significantly reduced.

<Experimental Example 3> Confirmation of inhibition of cytokine production

5% CO₂ incubator에서 24시간 배양하였다. 그 후, 미세먼지를 250㎍/㎖ 농도로 처리하고, 비교예 1, 2 및 실시예 1을 각각 200㎍/㎖ 농도로 처리하여 18시간 동안 CO₂ incubator에 배양한 후 배양 상층액을 희석하여 제조사의 지시에 따라 ELISA를 수행하였으며, 450nm 파장에서 마이크로스펙트로포토미터로 측정하였다. 최종 사이토카인의 양은 일정 단백질당 사이토카인 양으로 환산하여 음성대조군 (Control)과 비교하였다.In order to confirm the inhibition of inflammatory cytokine production of the phytoncide and natural extracts of Example 1 according to the present invention by adding hydrogen to the extract, HaCaT cells were dispensed into a ⁶ well plate (Corning, USA) to a cell number of 1 Х10 ⁶ cells/well By 37

Incubated for 24 hours in a 5% CO ₂ incubator. Thereafter, fine dust was treated at a concentration of 250 µg/ml, and Comparative Examples 1, 2 and Example 1 were each treated at a concentration of 200 µg/ml, incubated in a CO ₂ incubator for 18 hours, and then the culture supernatant was diluted. ELISA was performed according to the manufacturer's instructions, and measurements were made with a microspectrophotometer at 450 nm wavelength. The amount of the final cytokine was converted to the amount of cytokine per certain protein and compared with the negative control group (Control).

As a result, as shown in FIG. 4, hydrogen was added to the extract obtained by adding hydrogen to the phytoncide of Comparative Example 1, the extract obtained by adding hydrogen to the complex natural extracts of Comparative Example 2, and the phytoncide and natural extracts of Example 1 When one extract was treated, the amount of IL-6 increased by fine dust decreased to 157.6%, 143.8%, and 102.6%, respectively. In addition, when the amount of IL-6 increased by fine dust was 100%, Comparative Example 1 showed an inhibitory ability of 32.94% and Comparative Example 2 was 49.01%, and in particular Example 1 produced 96.97% of IL-6. It was confirmed that the inhibitory ability was shown, and IL-6 inhibitory ability was superior to 64.03% compared to Comparative Example 1 (extract by adding hydrogen to phytoncide) and 47.96% better than Comparative Example 2 (extract by adding hydrogen to complex natural extracts). .

As shown in FIG. 5, the extract obtained by adding hydrogen to the phytoncide of Comparative Example 1, the extract obtained by adding hydrogen to the composite natural extracts of Comparative Example 2, and the extract of adding hydrogen to the phytoncide and natural extracts of Example 1 were prepared. In the case of treatment, the amount of IL-8 increased by fine dust decreased to 131.8%, 135.5%, and 105.6%, respectively. In addition, when the amount of IL-8 increased by fine dust was 100%, Comparative Example 1 showed 34.7%, Comparative Example 2 showed 27.1% inhibitory ability, especially in Example 1 88.5% IL-8 production It was confirmed that the inhibitory ability was shown, and IL-8 inhibitory ability was more excellent than Comparative Example 1 (extract of adding hydrogen to phytoncide) by 53.8%, and 61.4% than Comparative Example 2 (extract by adding hydrogen to complex natural extracts). .

생성량 대비 각각 155.5%, 147.3%, 120.5%로 감소하였다. 또한, 미세먼지에 의해 증가한 IL-1

의 양을 100%라 하였을 때, 비교예 1은 31.9%, 비교예 2는 41.96%의 억제능을 보였으며, 특히 실시예 1에서 74.85%의 IL-1

생성 억제능을 보였으며, 비교예 1 (피톤치드에 수소를 첨가한 추출물) 보다 42.95%, 비교예 2 (복합 천연 추출물들에 수소를 첨가한 추출물) 보다 32.89% 더 우수한 IL-1

억제능을 보이는 것을 확인하였다. In addition, as shown in Fig. 6, the extract obtained by adding hydrogen to the phytoncide of Comparative Example 1, the extract of which hydrogen was added to the complex natural extracts of Comparative Example 2, and the phytoncide and natural extracts of Example 1 were added with hydrogen. When treating the extract, IL-1 increased by fine dust

The amount of production decreased to 155.5%, 147.3%, and 120.5%, respectively. In addition, IL-1 increased by fine dust

When the amount of is 100%, Comparative Example 1 showed an inhibitory ability of 31.9%, Comparative Example 2 was 41.96%, particularly in Example 1 74.85% of IL-1

It showed the ability to inhibit production, and was 42.95% better than Comparative Example 1 (extract by adding hydrogen to phytoncide) and 32.89% better than Comparative Example 2 (extract by adding hydrogen to complex natural extracts).

It was confirmed that it showed inhibitory ability.

생성량 대비 각각 200.1%, 165.8%, 116.5%로 감소하였다. 또한, 미세먼지에 의해 증가한 TNF-

의 양을 100%라 하였을 때, 비교예 1은 17.54%, 비교예 2는 45.8%의 억제능을 보였으며, 특히 실시예 1에서 86.41%의 TNF-

생성 억제능을 보였으며, 비교예 1 (피톤치드에 수소를 첨가한 추출물) 보다 68.87%, 비교예 2 (복합 천연 추출물들에 수소를 첨가한 추출물) 보다 40.61% 더 우수한 TNF-

억제능을 보이는 것을 확인하였다. As shown in FIG. 7, the extract obtained by adding hydrogen to the phytoncide of Comparative Example 1, the extract obtained by adding hydrogen to the complex natural extracts of Comparative Example 2, and the extract of adding hydrogen to the phytoncide and natural extracts of Example 1 were prepared. When treated, TNF- increased by fine dust

The amount of production decreased to 200.1%, 165.8%, and 116.5%, respectively. In addition, TNF- increased by fine dust

When the amount of is 100%, Comparative Example 1 was 17.54%, Comparative Example 2 showed 45.8% inhibitory ability, particularly in Example 1 86.41% TNF-

TNF- showed the ability to inhibit production, and was 68.87% better than Comparative Example 1 (extract by adding hydrogen to phytoncide) and 40.61% better than Comparative Example 2 (extract by adding hydrogen to composite natural extracts).

It was confirmed that it showed inhibitory ability.

So far, the present invention has been looked at around its preferred embodiments. Those of ordinary skill in the art to which the present invention pertains will be able to understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative point of view rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Manufacturing example of cosmetics

The extract obtained by adding hydrogen to the phytoncide and natural extracts according to the present invention can be prepared in various types of cosmetics depending on the purpose. The following exemplifies a method for preparing some cosmetics containing an extract obtained by adding hydrogen to phytoncide and natural extracts according to the present invention as an active ingredient, and the present invention is not limited thereto.

<Cosmetic Preparation Example 1> Preparation of flexible lotion

10.0 parts by weight of extract obtained by adding hydrogen to phytoncide and natural extracts

1,3-butylene glycol 1.00 parts by weight

0.05 parts by weight of disodium EDTA

0.10 parts by weight of allantoin

0.05 parts by weight of dipotassium glycyrrhizate

0.01 parts by weight of citric acid

Sodium citrate 0.02 parts by weight

Glycereth-26 1.00 parts by weight

2.00 parts by weight of arbutin

Hydrogenated castor oil 1.00 parts by weight

Ethanol 30.0 parts by weight

Trace amount of preservative

Trace amount of colorant

Trace amount of flavoring agent

Remaining amount of purified water

<Cosmetic Preparation Example 2> Preparation of nourishing cream

10.0 parts by weight of extract obtained by adding hydrogen to phytoncide and natural extracts

1,3-butylene glycol 7.00 parts by weight

1.00 parts by weight of glycerin

0.10 parts by weight of D-panthenol

3.20 parts by weight of plant extract

Magnesium aluminum silicate 0.30 parts by weight

1.20 parts by weight of PEG-40 stearate

2.00 parts by weight of stearic acid

1.50 parts by weight of polysorbate 60

2.00 parts by weight of lipophilic glyceryl stearate

1.50 parts by weight of sorbitan sesquioleate

3.00 parts by weight of cetearyl alcohol

4.00 parts by weight of mineral oil

3.80 parts by weight of squalane

2.80 parts by weight of carlylic/capric triglyceride

1.80 parts by weight of vegetable oil

Dimethicone 0.40 parts by weight

Trace amount of diposium glycyrrhizate

Trace amounts of allantoin

Sodium hyaluronate trace amount

Tocopheryl acetate appropriate amount

Appropriate amount of triethanolamine

Appropriate amount of preservative

Suitable amount of flavoring agent

Remaining amount of purified water

Claims (9)

In a cosmetic composition for preventing or improving skin inflammation due to fine dust, comprising hydrogen and phytoncide,
The cosmetic composition further comprises a complex natural extract composed of blueberries, black rice, licorice, black garlic and turmeric,
The hydrogen is subjected to ultrasonic treatment and degassing treatment conditions, and the ultrasonic treatment and degassing treatment conditions were carried out for 3 to 7 minutes at a frequency of 30 to 50 kHz and a vacuum pressure of 0.3 to 0.7 bar, preventing skin inflammation due to fine dust Or cosmetic composition for improvement.
delete delete The method of claim 1,
The cosmetic composition includes skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, foundation, essence, nutrition essence, pack, soap, cleansing A cosmetic composition for preventing or improving skin inflammation due to fine dust, characterized in that it is at least one formulation selected from a formulation consisting of foam, cleansing lotion, cleansing cream, body lotion and body cleanser.
The method of claim 1,
The cosmetic composition is a cosmetic for preventing or improving skin inflammation due to fine dust, characterized in that it suppresses the generation of NO overexpressed by fine dust and inhibits the production of IL-6, IL-8, IL-1β and TNF-α Composition.
delete delete The method of claim 1,
The cosmetic composition comprises the steps of preparing a complex natural extract consisting of blueberries, black rice, licorice, black garlic and turmeric (step 1);
Adding cypress phytoncide to the complex natural extract prepared in step 1 (step 2); And
Injecting high-concentration hydrogen obtained through ultrasonic treatment and degassing conditions for 3 to 7 minutes at a frequency of 30 to 50 kHz and a vacuum pressure of 0.3 to 0.7 bar to the composite natural extract prepared in step 2 to which cypress phytoncide was added A cosmetic composition for preventing or improving skin inflammation due to fine dust, characterized in that produced by a manufacturing method comprising a step (step 3).
The method of claim 8,
The extract is a cosmetic composition for preventing or improving skin inflammation due to fine dust, characterized in that the extract is extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

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