KR102070168B1 - Organic germanium assisted yeast fermentated material having skin whitening, skin antiwrinkle and antiinflammation effect - Google Patents
Organic germanium assisted yeast fermentated material having skin whitening, skin antiwrinkle and antiinflammation effect Download PDFInfo
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- KR102070168B1 KR102070168B1 KR1020180049721A KR20180049721A KR102070168B1 KR 102070168 B1 KR102070168 B1 KR 102070168B1 KR 1020180049721 A KR1020180049721 A KR 1020180049721A KR 20180049721 A KR20180049721 A KR 20180049721A KR 102070168 B1 KR102070168 B1 KR 102070168B1
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Abstract
본 발명은 미강 추출물과 귀리 추출물의 효모 발효 시, 유기게르마늄을 첨가하여 효모 발효시킴으로써 개선된 피부 미백, 피부 항주름 및 항염증 효능을 갖는 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물 및 그의 제조방법에 관한 것으로서, 본 발명에 따른 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물은 미강 추출물, 귀리 추출물, 당분, 비타민 B 복합 수용액 및 효모 추출물을 포함하는 원물 혼합물에 유기게르마늄을 첨가하고, 효모균을 접종하고, 배양시켜 수득된 것이다.The present invention is an organic germanium supplement having improved skin whitening, skin anti-wrinkle and anti-inflammatory effects by yeast fermentation by adding organic germanium at the time of yeast fermentation of rice bran extract and oat extract. The present invention relates to a yeast fermented product and a method for preparing the same, wherein the organic germanium auxiliary yeast fermented product having skin whitening, skin anti-wrinkle and anti-inflammatory effects according to the present invention comprises rice bran extract, oat extract, sugar, vitamin B complex aqueous solution and yeast extract. Organic germanium is added to the containing raw material mixture, inoculated with yeast, and cultured.
Description
본 발명은 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물 및 그의 제조방법에 관한 것으로 특히, 미강 추출물과 귀리 추출물의 효모 발효 시, 유기게르마늄을 첨가하여 효모 발효시킴으로써 개선된 피부 미백, 피부 항주름 및 항염증 효능을 갖는 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물 및 그의 제조방법에 관한 것이다. The present invention relates to an organic germanium supplementary yeast fermentation having a skin whitening, anti-wrinkle and anti-inflammatory effect and a method for preparing the same, and in particular, during yeast fermentation of rice bran extract and oat extract, it is improved by fermenting yeast by adding organic germanium. The present invention relates to an organic germanium supplemental yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory effects, and a method for preparing the same.
최근 생활수준이 향상됨에 따라 현대인들은 건강한 신체를 유지하는 것에 더하여 건강한 피부를 유지하는 데에도 많은 관심을 기울이고 있다. 따라서 피부미용 증진과 피부노화 방지에 대한 관심이 높아지고 있다.With the recent increase in living standards, modern people are paying more attention to maintaining healthy skin in addition to maintaining a healthy body. Therefore, interest in promoting skin beauty and preventing skin aging is increasing.
인체의 가장 큰 기관으로서 전체 인체 부피의 약 16%를 차지하고 있는 피부는 외부환경과 직접 접해 있으면서, 인체 안으로 침입하려는 치명적인 많은 유해인자, 예를 들면, 온도, 습도 및 자외선 등으로부터 인체를 보호하는 중요한 보호막 역할을 담당한다. 그러나, 나이가 들어감에 따라 각종 오염물질, 강한 자외선, 스트레스 및 영양결핍 등으로 인해 피부 세포들이 손상을 입게 되고, 세포 증식이 제대로 이루어지지 않게 되어 피부에 주름, 탄력 손실 및 각질화 등이 발생한다.As the largest organ of the human body, which occupies about 16% of the total body volume, the skin is in direct contact with the external environment and is important to protect the body from many deadly harmful factors such as temperature, humidity, and ultraviolet radiation. Act as a shield. However, with age, skin cells are damaged due to various contaminants, strong ultraviolet rays, stress, and malnutrition, and cell proliferation is not performed properly, resulting in wrinkles, loss of elasticity, and keratinization.
피부 노화는 크게 자연 노화 (혹은 내인성 노화)와 외적 노화로 구분되며 (Doris EB, Cosmet. Toiletries, 1996, 111, 31), 자연 노화 (내인성 노화)는 유전적인 요소에 영향을 받기 때문에 인위적인 조절이 어려운 반면 외적노화는 환경적인 요소에 영향을 받기 때문에 인위적인 조절이 비교적 용이하다. 대표적인 외적 노화 인자로는 자외선, 활성 산소종(reactive oxygen species) 및 스트레스 등으로, 가장 두드러진 외적인 노화 현상은 주름 형성이며(Daniell HW, Ann. Intern. Med., 1971, 75(6), 873; Grove GL 등, J. Am. Acad. Dermatol., 1989, 21(3), 631; Griffiths CE 등, Arch. Dermatol., 1992, 128, 347) 그 결과 피부 탄력이 현저히 감소하게 된다.Skin aging is largely divided into natural aging (or endogenous aging) and external aging (Doris EB, Cosmet. Toiletries, 1996, 111, 31), and because natural aging (endogenous aging) is affected by genetic factors, On the other hand, external aging is relatively easy to artificially adjust because of environmental factors. Representative external aging factors include ultraviolet light, reactive oxygen species and stress, and the most prominent external aging phenomenon is wrinkle formation (Daniell HW, Ann. Intern. Med., 1971, 75 (6), 873; Grove GL et al., J. Am. Acad. Dermatol., 1989, 21 (3), 631; Griffiths CE et al., Arch. Dermatol., 1992, 128, 347). As a result, skin elasticity is significantly reduced.
피부의 노화 기작을 규명하기 위한 여러 연구들이 진행되어 여러 가지 피부 노화의 원인들이 밝혀졌다. 피부가 온도의 변화, 습도의 저하 또는 바람 등에 의해 건조하게 되면 외부에 대한 방어벽으로서의 피부 생리 활성이 저하되어 노화가 촉진된다. 또한, 유해 활성 산소종 또는 자유 라디칼에 피부가 노출되면 체내의 산화 작용에 의해 과산화 지질이 생성되고 피부를 구성하는 여러 단백질들의 변형을 유발하게 된다. 자외선에 의해 야기되는 광노화 메커니즘 중의 하나는 자유 라디칼 경로를 경유하는 것이다 (Harman D,J. Gerontol., 1956, 11(3), 298). 자유 라디칼들은 피부 콜라겐 등의 결합 조직 형성 파괴, 세포막 기능저해, DNA 변이 촉진, 단백질 작용 변형, 세포간 에너지 전이, 신진 대사와 관련된 분자들의 변형 등을 유발하는 것으로 알려져 있다 (Lavker RM 및 Kligman AM, J. Invest. Dermatol., 1988, 90, 325). 노화에 자유 라디칼이 관여한다는 사실은 자유 라디칼을 불활성화시키는 항산화제 또는 여러가지 화학적 소거제들을 사용하여 노화 과정을 지연시킬 수 있다는 것을 의미한다.Various studies have been conducted to investigate the mechanism of aging of the skin, revealing various causes of skin aging. When the skin is dried by a change in temperature, a decrease in humidity or wind, the skin physiological activity as a protective barrier against the outside is lowered, thereby promoting aging. In addition, exposure of the skin to harmful reactive oxygen species or free radicals causes oxidative action in the body to produce lipid peroxide and cause modification of the various proteins that make up the skin. One of the photoaging mechanisms caused by ultraviolet light is via free radical pathways (Harman D, J. Gerontol., 1956, 11 (3), 298). Free radicals are known to cause breakdown of connective tissue formation such as skin collagen, cell membrane dysfunction, DNA mutation promotion, protein action alteration, intercellular energy transfer, metabolism related molecules (Lavker RM and Kligman AM, J. Invest.Drmatol., 1988, 90, 325). The fact that free radicals are involved in aging means that antioxidants or various chemical scavengers that inactivate the free radicals can be used to delay the aging process.
또한, 피부의 진피조직 속에는 콜라겐(collagen)과 피부의 탄력성에 관련된 엘라스틴(elastine)이 그물망 구조를 형성하고 있는데, 이러한 그물망 구조가 깨어지면서 즉, 엘라스틴이 엘라스틴 분해효소(elastase)에 의해 분해되어 피부가 처지고 주름이 생기므로 내인성 피부노화가 발생한다. 그러므로, 피부노화의 주원인 중의 하나인 엘라스틴 분해효소(elastase)의 활성을 저하시킴으로써 피부노화를 억제할 수 있다.In addition, collagen and elastine related to the elasticity of the skin form a mesh structure in the dermal tissue of the skin. As the mesh structure is broken, elastin is broken down by elastin degrading enzyme (elastase) And wrinkling causes endogenous skin aging. Therefore, skin aging can be suppressed by lowering the activity of elastinase, one of the main causes of skin aging.
종래에는 피부노화 방지를 위하여 거의 피부 표면에 바르는 화장품에 의존해왔다. 대한민국 공개특허공보 제2001-65755호에는 피부세포 증식, 콜라겐섬유 합성 촉진 등 피부 주름개선 및 탄력증진 효과가 있는 베타-1,3-글루칸과 인삼사포닌 함유 외용제 조성물이 개시되어 있으며, 대한민국 특허등록 제423,521호에는 주름개선 효과, 콜라겐 합성촉진효과, 광접촉에 의한 손상피부 회복 효과, 보습 효과를 나타내는 인삼다당체를 함유한 화장품 조성물이 개시되어 있다. 그러나 직접 피부 표면에 적용되는 외용제 및 화장품은 진피층까지 흡수되지 못하고, 표피에만 작용하므로 피부노화 방지효과가 일시적이고, 그 부작용으로 피부가 손상되기도 하는 문제점이 있다. 또한 체내에 있는 활성산소를 제거하지 못하여, 활성산소에 의한 노화작용을 막기에는 한계가 있었다.Conventionally, it has been relied on cosmetics that are almost applied to the skin surface to prevent skin aging. Korean Unexamined Patent Publication No. 2001-65755 discloses an external preparation composition containing beta-1,3-glucan and ginseng saponin which has an effect of improving skin wrinkles and promoting elasticity such as skin cell proliferation and collagen fiber synthesis. No. 423,521 discloses a cosmetic composition containing ginseng polysaccharides that exhibits an anti-wrinkle effect, a collagen synthesis promoting effect, a damaged skin recovery effect by optical contact, and a moisturizing effect. However, the external preparations and cosmetics directly applied to the skin surface are not absorbed up to the dermal layer and act only on the epidermis, so that the skin aging prevention effect is temporary and the skin may be damaged by the side effects. In addition, there is a limit to prevent the aging action by the active oxygen because it can not remove the active oxygen in the body.
또한, 화학물질로 이루어진 미용 식품 조성물들은 그 부작용이 있어 많은 폐단을 초래하는 이유로, 천연물질 등을 이용하여 장기간 사용 시에도 안정성이 높은 피부노화 방지 및 개선 물질을 개발하의 필요성이 절실히 요구되고 있는 실정이다.In addition, the cosmetic food compositions made of chemicals have a lot of side effects because of the side effects, the use of natural materials, such as the need for the development of highly stable skin aging prevention and improvement material even in the long-term use is urgently required. to be.
대한민국 등록특허 등록번호 제10-1187227호(발명의 명칭: 유산균과 효모의 복발효법을 이용한 미나리음료 및 그 제조방법)는 "본 발명은 젖산균과 효모를 이용한 복발효를 통해 건강에 도움이 되는 폴리페놀 성분을 포함하는 미나리를 이용하는 전혀 새로운 종류의 유산균과 효모의 복발효법을 이용한 미나리음료 및 그 제조방법에 관한 것으로서, 미나리를 분쇄하고, 젖산균과 효모를 준비한 다음, 분쇄된 미나리에 젖산균을 접종하여 일차 발효를 수행하고, 일차 발효가 끝난 배양액에 효모를 접종하여 이차 발효를 수행하여 복발효된 미나리 음료를 얻게 된다."고 기술하고 있다.Republic of Korea Patent Registration No. 10-1187227 (name of the invention: the buttercup beverage using lactic acid bacteria and yeast bifermentation method and its manufacturing method) "The present invention is a poly to help health through the double fermentation using lactic acid bacteria and yeast The present invention relates to a buttercup beverage using a novel fermentation method of lactic acid bacteria and yeast using a buttercup containing a phenol component, and to a method of manufacturing the same. The method is prepared by crushing buttercups, preparing lactic acid bacteria and yeast, and then inoculating the powdered buttercups with lactic acid bacteria. The primary fermentation is carried out, and the fermented broth is inoculated with yeast to obtain secondary fermented buttercup beverages. ”
대한민국 등록특허 등록번호 제10-1177915호(발명의 명칭: 시금치 추출물의 효모발효액을 함유하는 주름 예방 및 노화방지용 피부외용제 조성물)는 "본 발명은 시금치 추출물에 효모를 접종하고 배양시켜 얻은 시금치 추출물의 효모발효액을 활성성분으로 함유하는 주름 예방 및 노화 방지용 피부외용제 조성물에 관한 것이다. 상기 시금치 추출물의 효모발효액은 프로콜라겐(procollagen)의 합성을 촉진하고 콜라게네이즈(collagenase)의 생성을 억제하는 효과가 있다."고 기술하고 있다.Republic of Korea Patent Registration No. 10-1177915 (name of the invention: anti-wrinkle and anti-aging skin external composition comprising a yeast fermentation of spinach extract) "The present invention is a spinach extract obtained by inoculating and incubating The present invention relates to an anti-wrinkle and anti-aging skin external composition comprising a yeast fermentation solution as an active ingredient The yeast fermentation solution of the spinach extract has an effect of promoting the synthesis of procollagen and inhibiting the production of collagenase. It is. "
대한민국 등록특허 등록번호 제10-1824179호(발명의 명칭: 더위지기 효모 발효물을 포함하는 보습 및 항염증 화장료 조성물)는 "본 발명은 우수한 보습 및 항염증 효능을 갖는 더위지기 효모 발효물을 포함하는 화장료 조성물 및 그 제조방법에 관한 것으로, 보다 구체적으로는 더위지기를 효모발효시킨 발효물을 화장료 조성물 내 유효성분으로 포함함으로써, 발효 전보다 피부자극이 감소하며, 증가된 보습 효과, 항산화 효과 및 항염증 효과를 갖는 것이다."고 기술하고 있다.Republic of Korea Patent Registration No. 10-1824179 (name of the invention: moisturizing and anti-inflammatory cosmetic composition comprising heat keeper yeast fermentation) "The present invention includes a heat keeper yeast fermentation having excellent moisturizing and anti-inflammatory efficacy The present invention relates to a cosmetic composition and a method for preparing the same, and more specifically, to include a fermented product obtained by fermenting a heat-treated yeast as an active ingredient in the cosmetic composition, thereby reducing skin irritation than before fermentation, and increasing moisturizing effect, antioxidant effect and antibacterial effect. It has an inflammatory effect. "
본 발명은 미강 추출물과 귀리 추출물의 효모 발효 시, 유기게르마늄을 첨가하여 효모 발효시킴으로써 개선된 피부 미백, 피부 항주름 및 항염증 효능을 갖는 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물 및 그의 제조방법을 제공함을 목적으로 한다.The present invention is an organic germanium supplement having improved skin whitening, skin anti-wrinkle and anti-inflammatory effects by yeast fermentation by adding organic germanium at the time of yeast fermentation of rice bran extract and oat extract. It is an object to provide a yeast fermented product and a method for producing the same.
본 발명에 따른 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물은 미강 추출물, 귀리 추출물, 당분, 비타민 B 복합 수용액 및 효모 추출물을 포함하는 원물 혼합물에 유기게르마늄을 첨가하고, 효모균을 접종하고, 배양시켜 수득된 것이다.The organic germanium auxiliary yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory efficacy according to the present invention is added to the organic mixture of the raw material mixture, including rice bran extract, oat extract, sugar, vitamin B complex aqueous solution and yeast extract, Obtained by inoculating and culturing yeast.
상기 효모균은 사카로마이세스 속(Saccharomyces sp .), 데브리오마이세스 속(Debaryomyces sp .) 및 아우레오바시둠 속(Aureobasidum sp .)으로 이루어지는 군으로부터 선택되는 효모일 수 있다.The yeast is Saccharomyces sp . ), Debaryomyces sp . And Aureobasidum sp . Yeast selected from the group consisting of
상기 효모균은 사카로마이세스 폼베(Saccharomyces pombe) 균일 수 있다.The yeast is Saccharomyces pombe ) can be uniform.
상기 유기게르마늄은 레파게르마늄, 프로파게르마늄 또는 이들의 혼합물일 수 있다.The organic germanium may be lepagermanium, propagernium or a mixture thereof.
상기 원물 추출물에의 유기게르마늄의 첨가량은 원물 추출물 총 중량을 기준으로 0.1 내지 10중량%의 범위 이내일 수 있다.The amount of the organic germanium added to the raw extract may be in the range of 0.1 to 10% by weight based on the total weight of the raw extract.
본 발명에 따른 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물 함유 화장품은 상기 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물을 유효성분으로 포함한다.The organic-germanium auxiliary yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory effects according to the present invention comprises the organic germanium auxiliary yeast fermentation having the skin whitening, skin anti-wrinkle and anti-inflammatory effects as an active ingredient. .
본 발명에 따른 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물 함유 건강기능식품은 상기 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물을 유효성분으로 포함한다.The health functional food containing organic germanium supplementary yeast fermentation having skin whitening, skin anti-wrinkle and anti-inflammatory effects according to the present invention is an organic germanium supplementary yeast fermentation having the skin whitening, skin anti-wrinkle and anti-inflammatory effect as an active ingredient Include.
본 발명에 따른 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물의 제조방법은 (1) 미강 추출물, 귀리 추출물, 당분, 비타민 B 복합 수용액 및 효모 추출물을 포함하는 원물 혼합물을 준비하는 혼합단계; (2) 상기 원물 혼합물에 유기게르마늄을 첨가하는 유기게르마늄 첨가단계; 및 (3) 유기게르마늄이 첨가된 원물 혼합물에 효모균을 접종하고, 배양시키는 배양단계;를 포함한다.The method for preparing an organic germanium supplementary yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory effects according to the present invention comprises (1) preparing a raw mixture comprising rice bran extract, oat extract, sugar, aqueous solution of vitamin B complex and yeast extract Mixing step; (2) an organic germanium addition step of adding organic germanium to the raw material mixture; And (3) incubating the inoculated yeast with the original mixture to which the organic germanium is added and culturing.
본 발명에 따르면, 미강 추출물과 귀리 추출물의 효모 발효 시, 유기게르마늄을 첨가하여 효모 발효시킴으로써 개선된 피부 미백, 피부 항주름 및 항염증 효능을 갖는 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물 및 그의 제조방법을 제공하는 효과가 있으며, 이는 화장품 등의 원료로 사용될 수 있다.According to the present invention, in yeast fermentation of rice bran extract and oat extract, the organic whitening, skin anti-wrinkle and anti-inflammatory efficacy of organic whitening, skin anti-wrinkle and anti-inflammatory efficacy by adding organic germanium There is an effect of providing a germanium auxiliary yeast fermentation and a method for producing the same, which can be used as a raw material for cosmetics and the like.
도 1은 본 발명의 실시예 1에서 수득되는 유기게르마늄 보조 효모 발효물의 여과물(이하 "유기게르마늄-효모 발효물"이라 함)의 세포독성을 실험하기 위하여, Fibroblast CCD986-sk 세포주(A)와 Macrophage Raw264.7 세포주(B)에 유기게르마늄-효모 발효물을 농도별로 처리하여 MTT assay를 시행한 결과를 나타낸 도면이다.
도 2는 본 발명의 유기게르마늄-효모 발효물(A)과 효모발효 여과물(B: 유기게르마늄을 수반함이 없이 효모 발효된 발효물의 여과물), 유기게르마늄으로서 레파게르마늄 수용액(C)의 프로콜라겐 합성능을 비교 실험하기 위하여, Fibroblast CCD986-sk 세포주에 유기게르마늄-효모 발효물을 농도별로 처리하여 프로콜라겐을 측량한 결과를 나타낸 도면이다.
도 3은 본 발명의 유기게르마늄-효모 발효물(A)과 효모발효 여과물(B), 유기게르마늄으로서 레파게르마늄 수용액(C)의 멜라닌 생성 억제능을 비교 실험하기 위하여, melanoma B16F10 세포주에 유기게르마늄-효모 발효물을 농도별로 처리하여 생성된 멜라닌을 정량한 결과를 나타낸 도면이다.
도 4는 본 발명의 유기게르마늄-효모 발효물(A)과 효모발효 여과물(B), 유기게르마늄으로서 레파게르마늄 수용액(C)의 항염증능을 비교 실험하기 위하여, Macrophage Raw264.7 세포주에 유기게르마늄-효모 발효물을 농도별로 처리하여 TNF-α의 분비량을 측정한 결과를 나타낸 도면이다.1 is a fibroblast CCD986-sk cell line (A) for experimenting with the cytotoxicity of the filtrate of the organic germanium auxiliary yeast fermentation obtained in Example 1 of the present invention (hereinafter referred to as "organic germanium- yeast fermentation") Macrophage Raw264.7 cell line (B) is a diagram showing the results of MTT assay by treating the organic germanium- yeast fermentation by concentration.
Figure 2 is an organic germanium- yeast fermentation product (A) of the present invention and the yeast fermentation filtrate (B: the filtrate of yeast fermentation without the accompanying organic germanium), the proger of the aqueous solution of lepagermanium (C) as organic germanium In order to compare the collagen synthesis ability, it is a diagram showing the results of procollagen measurement by treating the organic germanium- yeast fermentation by concentration in the Fibroblast CCD986-sk cell line.
Figure 3 is an organic germanium- yeast fermentation product (A) of the present invention and the yeast fermentation filtrate (B), in order to compare the melanin production inhibitory ability of the aqueous solution of lepagermanium (C) as organic germanium, organic germanium-in the melanoma B16F10 cell line Figure showing the results of quantifying the melanin produced by treating the yeast fermentation by concentration.
Figure 4 is an organic germanium- yeast fermentation product (A) of the present invention, and the yeast fermentation filtrate (B), in order to compare the anti-inflammatory activity of the aqueous solution of lepagermanium (C) as organic germanium, organic to Macrophage Raw264.7 cell line It is a figure which shows the result of measuring the secretion amount of TNF- (alpha) by processing germanium yeast fermentation by concentration.
이하, 본 발명을 구체적인 실시예를 참조하여 상세히 설명한다.Hereinafter, the present invention will be described in detail with reference to specific examples.
본 발명에 따른 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물은 미강 추출물, 귀리 추출물, 당분, 비타민 B 복합 수용액 및 효모 추출물을 포함하는 원물 혼합물에 유기게르마늄을 첨가하고, 효모균을 접종하고, 배양시켜 수득된 것임을 특징으로 한다.The organic germanium auxiliary yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory efficacy according to the present invention is added to the organic mixture of the raw material mixture, including rice bran extract, oat extract, sugar, vitamin B complex aqueous solution and yeast extract, It is characterized in that obtained by inoculating and culturing yeast.
상기 효모균은 사카로마이세스 속(Saccharomyces sp .), 데브리오마이세스 속(Debaryomyces sp .) 및 아우레오바시둠 속(Aureobasidum sp .)으로 이루어지는 군으로부터 선택되는 효모일 수 있으며, 바람직하게는 상기 효모균은 사카로마이세스 폼베(Saccharomyces pombe) 균일 수 있다.The yeast is Saccharomyces sp . ), Debaryomyces sp . And Aureobasidum sp . It may be a yeast selected from the group consisting of, preferably the yeast may be Saccharomyces pombe ( Saccharomyces pombe ) uniform.
상기 유기게르마늄은 레파게르마늄, 프로파게르마늄 또는 이들의 혼합물일 수 있다. 유기게르마늄에 대하여 알려진 일반적인 효능은 다음과 같다. The organic germanium may be lepagermanium, propagernium or a mixture thereof. The general efficacy known for organogerium is as follows.
1. 산소 공급작용1. Oxygen supply
인체 세포들이 신진대사를 계속 반복적으로 행하려면 영양소와 산소가 절대 필요하며, 산소부족 시 만성 일산화탄소 중독증, 빈혈, 혈관장애, 심장장애, 저혈압, 세포노화, 정신장애 등의 질환이 발생한다. 최근에 와서 의학자들의 실험에 의해 게르마늄은 산소의 효율적인 활용을 돕는 산소촉매제의 역할을 한다는 사실이 밝혀졌다. 즉, 인체에 유기게르마늄을 공급하게 되면 여분의 산소는 혈액에 생명력을 공급하기 때문에 만성산소결핍 현상에서 벗어나게 된다고 알려지고 있다.Human cells need nutrients and oxygen in order to continue to metabolize repeatedly, and when oxygen lacks, diseases such as chronic carbon monoxide poisoning, anemia, vascular disorders, heart disorders, hypotension, cell aging, and mental disorders occur. In recent years, medical scientists have found that germanium acts as an oxygen catalyst to help the efficient use of oxygen. In other words, when the organic germanium is supplied to the human body, it is known that extra oxygen supplies vitality to the blood, thereby releasing chronic oxygen deficiency.
2. 반도체작용(체내세포의 전류 흐름조절작용): 음이온 효과2. Semiconductor action (regulation of current flow in body cells): Anion effect
게르마늄의 최대 특징은 전기적인 성질로 금속과 비금속의 중간적 성질을 갖고 있는 반도체인 것이며, 게르마늄의 이런 성질이 바로 인체의 온갖 질병을 치료해 주는 원리가 된다고 하여 기적의 원소라고 불린다. 만일 체내에 이물질이 생기게 되면 4개의 전자 중 바깥쪽 전자가(-)상태가 되어 밖으로 튀어나오고 나머지는 3개는 (+)의 하전상태가 되어 신체와 조화를 이루게 된다. 게르마늄의 이런 역할이 바로 신체의 질병을 치료해주는 원리가 되며, 한방에서 경락과 경혈에 행하는 침이나 뜸, 지압도 마찬가지로 세포의 반도체 흐름을 원활히 하여 질병을 치료하는 똑같은 원리인 것이다.The biggest feature of germanium is the semiconductor, which is an electrical property and has the intermediate property between metal and nonmetal. It is called a miracle element because this property of germanium is the principle that heals all kinds of diseases of the human body. If a foreign body is created in the body, the outer electrons of the four electrons become (-) state and pop out, and the other three are positively charged state and are in harmony with the body. This role of germanium is the principle that heals diseases of the body, and the acupuncture, moxibustion, and acupressure of the meridians and acupuncture points in the same medicine is the same principle of treating diseases by smoothing the semiconductor flow of cells.
3. 면역력 강화 작용3. strengthening immunity
인체는 크게 2가지의 면역체계를 가지고 있는데, 첫째는 백혈구에 의한 것으로 체내에 들어온 병원균을 식균작용에 의해 잡아먹는 체계이고, 둘째는 항체에 의한 작용인데 T-임파구에 의해 B-임파구가 형성되어 병원균과 결합하여 그 균을 죽이는 작용을 하는 체계이다. 따라서 암에 걸렸다고 하는 것은 T-임파구 수가 저하되었다는 것을 의미하여 게르마늄은 T-임파구를 증식시켜 암세포, 독성물질, 바이러스 등으로부터 신체를 보호한다. 또한 일본 동경대학교 항산 균병 연구소의 사토박사는 유기게르마늄(Ge-132)의 임상실험에서도 유기게르마늄은 B.R.M(생체방어기구 활성화 물질)의 하나로써 면역조절에 탁월한 효과가 있다고 밝혔다.Human body has two kinds of immune system. First, it is caused by leukocytes, and it is a system that eats pathogens that enter the body by phagocytosis. Second, it is caused by antibodies. B-lymphocytes are formed by T-lymphocytes. It is a system that works by combining pathogens and killing them. Therefore, having cancer means that the number of T-lymphocytes has decreased, and germanium proliferates T-lymphocytes to protect the body from cancer cells, toxic substances, and viruses. In addition, Dr. Sato of the Tokyo Institute of Antibacterial and Bacterial Research at the University of Tokyo said that organic germanium is one of B.R.M (bioprotective device activating substances) and has an excellent effect on immunomodulation in clinical trials of organic germanium (Ge-132).
즉, 유기게르마늄은 생체에 대하여 긍정적인 영향을 주는 것으로 알려져 있으며, 본 발명에서는 이러한 유기게르마늄을 이용하여 효모 발효를 촉진하고 유효 생리활성물질의 생성을 촉진하는 것으로 여겨진다.In other words, it is known that organic germanium has a positive effect on the living body, and in the present invention, it is believed that the use of such organic germanium promotes fermentation of yeast and the production of effective bioactive substances.
상기 원물 혼합물은 비타민B1 0.1 내지 10중량%, 비타민 B2 0.01 내지 1중량% 및 비타민B2, 비타민B3 0.1 내지 10중량% 및 잔량으로서 물을 포함하여 이루어지는 비타민 B 복합 수용액을 더 포함할 수 있다.The raw material mixture may further include a vitamin B complex aqueous solution containing 0.1 to 10% by weight of vitamin B1, 0.01 to 1% by weight of vitamin B2, and 0.1 to 10% by weight of vitamin B2 and vitamin B3 and the balance.
상기 미강 추출물 및 귀리 추출물은 미강과 귀리를 열수추출한 것일 수 있다. 미강 추출물과 귀리 추출물을 제조하기 위하여는, 미강과 귀리를 각각 세척, 건조하여 해당 시료 무게의 2 내지 10배의 증류수로 25 내지 100℃에서 2 내지 6시간 동안 열수 추출하는 것으로 수행될 수 있다.The rice bran extract and oat extract may be hot water extracted rice bran and oats. To prepare the rice bran extract and the oat extract, washing and drying the rice bran and oats, respectively, may be performed by hot water extraction at 25-100 ° C. for 2-6 hours with distilled water of 2-10 times the weight of the sample.
상기 당분은 꿀일 수 있다.The sugar may be honey.
상기 원물 혼합물은 미강 추출물 1 내지 10중량%, 귀리 추출물 5 내지 30중량%, 당분 1 내지 3중량%, 비타민 B 복합 수용액 1 내지 1중량%, 효모 추출물 0.05 내지 0.2중량% 및 잔량으로서 물을 포함하여 이루어질 수 있다.The raw material mixture contains 1 to 10% by weight of rice bran extract, 5 to 30% by weight of oat extract, 1 to 3% by weight of sugar, 1 to 1% by weight of a vitamin B complex aqueous solution, 0.05 to 0.2% by weight of yeast extract and the balance as water. It can be done by.
상기 원물 추출물에의 유기게르마늄의 첨가량은 원물 추출물 총 중량을 기준으로 0.1 내지 10중량%의 범위 이내일 수 있다.The amount of the organic germanium added to the raw extract may be in the range of 0.1 to 10% by weight based on the total weight of the raw extract.
상기 원물 추출물에의 사카로마이세스 폼베(Saccharomyces pombe) 균의 접종량은 사카로마이세스 폼베 균을 YM배지에 접종하여 600㎚에서 1.0의 흡광도 값을 나타낼 때까지 확대배양한 배양액 5 내지 20중량%의 범위 이내일 수 있다. Saccharomyces on the Raw Material Extracts pombe ) was inoculated with Saccharomyces pombe on YM medium and expanded until absorbance values of 600 nm and 1.0 were obtained. The culture may be in the range of 5 to 20% by weight.
본 발명에 따른 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물 함유 화장품은 상기 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물을 유효성분으로 포함한다.The organic-germanium auxiliary yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory effects according to the present invention comprises the organic germanium auxiliary yeast fermentation having the skin whitening, skin anti-wrinkle and anti-inflammatory effects as an active ingredient. .
본 발명에 따른 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물 함유 건강기능식품은 상기 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물을 유효성분으로 포함한다.The health functional food containing organic germanium supplementary yeast fermentation having skin whitening, skin anti-wrinkle and anti-inflammatory effects according to the present invention is an organic germanium supplementary yeast fermentation having the skin whitening, skin anti-wrinkle and anti-inflammatory effect as an active ingredient Include.
본 발명에 따른 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물의 제조방법은 (1) 미강 추출물, 귀리 추출물, 당분, 비타민 B 복합 수용액 및 효모 추출물을 포함하는 원물 혼합물을 준비하는 혼합단계; (2) 상기 원물 혼합물에 유기게르마늄을 첨가하는 유기게르마늄 첨가단계; 및 (3) 유기게르마늄이 첨가된 원물 혼합물에 효모균을 접종하고, 배양시키는 배양단계;를 포함한다.The method for preparing an organic germanium supplementary yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory effects according to the present invention comprises (1) preparing a raw mixture comprising rice bran extract, oat extract, sugar, aqueous solution of vitamin B complex and yeast extract Mixing step; (2) an organic germanium addition step of adding organic germanium to the raw material mixture; And (3) incubating the inoculated yeast with the original mixture to which the organic germanium is added and culturing.
이하에서 본 발명의 바람직한 실시예 및 비교예들이 기술되어질 것이다.Hereinafter, preferred embodiments and comparative examples of the present invention will be described.
이하의 실시예들은 본 발명을 예증하기 위한 것으로서 본 발명의 범위를 국한시키는 것으로 이해되어져서는 안될 것이다.The following examples are intended to illustrate the invention and should not be understood as limiting the scope of the invention.
<실시예 1. 유기게르마늄-효모 발효물의 제조>Example 1 Preparation of Organic Germanium-Yeast Fermentation
미강 추출물과 귀리 추출물을 제조하기 위하여, 미강과 귀리를 각각 세척, 건조하여 해당 시료 무게의 4배량의 증류수로 실온(20 내지 30℃)에서 4시간 동안 열수추출하였다. 이후, 각 추출물을 10,000rpm에서 20분간 원심분리하여 상등액을 취하고, 이를 여과하여 사용하였다. 비타민B 복합 수용액은 5중량% 비타민B1, 1중량% 비타민B2, 5중량% 비타민B3 및 잔량으로서 물을 혼합하였다. 최종적으로, 5중량%의 미강 추출물, 20중량%의 귀리 추출물, 5중량%의 비타민B 복합 수용액, 2중량%의 꿀, 0.1중량%의 효모 추출물, 5중량%의 레파게르마늄, 10중량%의 사카로마이세스 폼베(Saccharomyces pombe) 균 배양액과 배합하여, 37℃에서 4일 동안 발효하고, 10,000rpm에서 10분 동안 원심분리하여 얻은 상등액을 여과하여 유기게르마늄-효모 발효물을 제조하였다.To prepare rice bran extract and oat extract, rice bran and oats were washed and dried, respectively, and hot water extracted for 4 hours at room temperature (20 to 30 ° C.) with distilled water of 4 times the weight of the sample. Then, each extract was centrifuged at 10,000 rpm for 20 minutes to take a supernatant, which was used by filtration. The vitamin B complex aqueous solution was mixed with 5 wt% vitamin B 1, 1 wt% vitamin B 2, 5 wt% vitamin B 3 and water as a balance. Finally, 5% by weight of rice bran extract, 20% by weight of oat extract, 5% by weight of vitamin B complex aqueous solution, 2% by weight of honey, 0.1% by weight of yeast extract, 5% by weight of repagermanium, 10% by weight of Saccharomyces pombe ) was combined with the culture medium, fermented for 4 days at 37 ℃, the supernatant obtained by centrifugation for 10 minutes at 10,000rpm was filtered to prepare an organic germanium- yeast fermentation.
<실험예 1. 유기게르마늄-효모 발효물의 세포독성>Experimental Example 1. Cytotoxicity of Organic Germanium-Yeast Fermentation>
Fibroblast 세포 및 macrophage 세포에 대한 유기게르마늄-효모 발효물의 세포독성을 알아보기 위하여 MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) 시약을 사용하여 측정하였다. Fibroblast 세포 및 macrophage 세포(2X104cells)에 유기게르마늄-효모 발효물을 각 0, 50, 100, 250ppm이 되도록 가하여 37℃에서 24시간 배양하였다. MTT 용액을 최종 농도 1㎎/㎖이 되도록 첨가하여 4시간 배양한 후, 배지를 제거하고 색소를 디메틸설폭사이드(DMSO)로 용해시킨 후, 570㎚에서 흡광도를 측정하였다. 세포생존율은 하기 수학식 1에 대입하여 계산하였다.To determine the cytotoxicity of organogermanium-yeast fermentation on fibroblast cells and macrophage cells, MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) reagent was used. Organic germanium-yeast fermentation was added to Fibroblast cells and macrophage cells (2X10 4 cells) to 0, 50, 100 and 250 ppm, respectively, and incubated at 37 ° C for 24 hours. After adding the MTT solution to a final concentration of 1 mg / ml and incubating for 4 hours, the medium was removed, the pigment was dissolved in dimethyl sulfoxide (DMSO), and the absorbance was measured at 570 nm. Cell viability was calculated by substituting Equation 1 below.
[수학식 1][Equation 1]
세포 생존율(%) = (실험군의 흡광도/대조군의 흡광도) X 100% Cell viability = (absorbance of experimental group / absorbance of control group)
상기 실험 결과, 도 1에 나타난 바와 같이, 유기게르마늄-효모 발효물은 해당 농도에서 세포독성이 없는 것으로 나타났다.As a result of the experiment, as shown in Figure 1, the organic germanium- yeast fermentation was found to be cytotoxic at the corresponding concentration.
<실험예 2. 유기게르마늄-효모발효 여과물의 프로콜라겐 합성능>Experimental Example 2. Procollagen Synthesis of Organic Germanium-Yeast Fermented Filtrate>
본 발명의 유기게르마늄-효모 발효물의 프로콜라겐 합성능을 실험하고자, procollagen Type I C-Peptide (PIP) EIA Kit (TAKARA)를 사용하여 프로콜라겐의 합성능을 측정하였다. Human fibroblast CCD986-sk 세포주를 48웰 배양접시에 5X104cells/웰로 24시간 동안 전 배양한 후, 0, 50, 100, 250ppm의 유기게르마늄-효모 발효물과 유기게르마늄이 포함되지 않은 효모발효 여과물, 0.5% 레파게르마늄 수용액을 각각 24시간 처리하였고, 이때 양성 대조군으로 100ng/㎖ TGFβ를 사용하였다. 이후, 0.5% Triton X-100이 함유된 PBS를 사용하여 세포를 용해하고, 프로콜라겐의 양을 procollagen Type I C-Peptide (PIP) EIA Kit로 측량하여 도 2에 나타내었다.To test the procollagen synthesis ability of the organic germanium-yeast fermentation of the present invention, the procollagen type I C-Peptide (PIP) EIA Kit (TAKARA) was used to measure the synthesis ability of procollagen. Human fibroblast CCD986-sk cell line was pre-incubated for 24 hours at 5X10 4 cells / well in a 48 well dish, followed by 0, 50, 100, 250 ppm of organic germanium-yeast fermentation and yeast fermentation-free yeast fermentation filtrate. , 0.5% aqueous solution of lepagermanium was treated for 24 hours, at which time 100ng / ml TGFβ was used as a positive control. Then, cells were lysed using PBS containing 0.5% Triton X-100, and the amount of procollagen was measured by procollagen Type I C-Peptide (PIP) EIA Kit, and is shown in FIG. 2.
상기 실험 결과, 도 2에 나타난 바와 같이, 유기게르마늄-효모 발효물의 제조물과 효모발효 여과물은 250ppm에서 최대 약 1.5배의 프로콜라겐 생합성을 촉진하였고, 0.5% 레파게르마늄 수용액은 프로콜라겐 합성능에 영향을 주지 않았다.As a result of the experiment, as shown in Figure 2, the preparation and the yeast fermentation product of the organic germanium- yeast fermentation promoted up to about 1.5-fold procollagen biosynthesis at 250ppm, 0.5% aqueous solution of lepagermanium affects the procollagen synthesis capacity Did not give.
<실험예 3. 레파게르마늄-효모발효 여과물의 멜라닌 합성 저해능>Experimental Example 3. Inhibition of Melanin Synthesis of Lepagermanium-Yeast Fermented Filtrate>
본 발명의 유기게르마늄-효모 발효물의 멜라닌 합성 저해능을 확인하고자 세포 내 멜라닌을 측량하였다. Melanoma B16F10 세포주를 2X105cell/웰이 들어가도록 6웰 플레이트에 분주하고 24시간 전 배양하였다. 배양 후, 배지를 제거하고, Phenol red free DMEM 배지에 α-MSH 100nM과 유기게르마늄-효모 발효물 및 효모발효 여과물, 0.5% 레파게르마늄 수용액을 농도별로 0, 50, 100, 250ppm을 처리하였다. 96시간 동안 배양한 후, 배지를 제거하고, PBS로 씻어준 다음 Trypsin-EDTA를 처리하고 2000rpm으로 2분동안 원심분리하여 세포를 얻었다. 이 세포에 10% DMSO가 들어간 1N NaOH를 200㎖ 넣고 교반한 후, 80℃에서 1시간 반응시키고, 96웰 플레이트에 100㎖씩 옮겨 405㎚에서 흡광도를 측정하였다. 위 용액을 단백질 정량하여 일정 단백질 당 멜라닌 값을 비교하여 도 3에 나타내었다.In order to determine the melanin synthesis inhibitory ability of the organic germanium- yeast fermentation of the present invention, the melanin in the cell was measured. Melanoma B16F10 cell line was aliquoted into 6-well plates containing 2 × 10 5 cells / well and incubated 24 hours ago. After incubation, the medium was removed, and 0, 50, 100, and 250 ppm of α-MSH 100nM, an organic germanium- yeast fermentation product, a yeast fermentation filtrate, and a 0.5% aqueous solution of lepagermanium were treated in Phenol red free DMEM medium. After incubation for 96 hours, the medium was removed, washed with PBS, treated with Trypsin-EDTA, and centrifuged at 2000 rpm for 2 minutes to obtain cells. 200 ml of 1N NaOH containing 10% DMSO was added to the cells, followed by stirring. The cells were reacted at 80 ° C for 1 hour, and then transferred to a 96-well plate in 100 ml portions, and the absorbance was measured at 405 nm. The above solution is shown in Figure 3 by comparing the melanin value per protein by protein quantification.
상기 실험 결과, 도 3에 나타난 바와 같이, 유기게르마늄-효모 발효물은 농도 의존적으로 100nM α-MSH에 의해 생성되는 멜라닌의 양을 감소 시켰으며, 0.5% 레파게르마늄 수용액은 250ppm에서 유의하게 α-MSH에 의해 생성되는 멜라닌의 양을 감소시켰다. 또한, 유기게르마늄-효모 발효물은 50ppm에서 α-MSH에 의해 생성되는 멜라닌의 양을 대조군 수준으로 감소시켰으며, 이는 효모발효 여과물(유기게르마늄으로 처리되지 않은)의 250ppm보다 우수하였다.As a result, as shown in Figure 3, the organic germanium- yeast fermentation reduced the amount of melanin produced by 100 nM α-MSH concentration-dependently, 0.5% aqueous solution of lepagermanium significantly α-MSH at 250ppm Reduced the amount of melanin produced by In addition, the organic germanium-yeast fermentation reduced the amount of melanin produced by α-MSH at 50 ppm to control levels, which was better than 250 ppm of yeast fermentation filtrate (not treated with organic germanium).
<실험예 4. 레파게르마늄-효모발효 여과물의 항염증능>Experimental Example 4. Anti-inflammatory Activity of Repagermanium-Yeast Fermented Filtrate>
본 발명의 유기게르마늄-효모 발효물의 항염증능 실험을 위해 TNF-α를 측량하였다. 마우스 대식세포 Raw264.7 세포주를 12 well cell culture plate에 1X106cells/웰로 분주하여 24 시간동안 전 배양 하였다. 24 시간 후 전 배양에 사용된 배지를 제거하고, 유기게르마늄-효모 발효물 및 효모발효 여과물, 0.5% 레파게르마늄 수용액을 0, 25, 50, 100ppm의 각 농도별로 첨가시킨 배지로 교체해 준 후 10분동안 배양하고, Normal 대조군을 제외한 나머지 시료 실험군에 LPS를 100ng/㎖ 농도로 처리하여서 20 시간 배양하여 염증을 유도하였다. 이후, 해당세포 배양액을 단백질 정량하여 실험에 사용하였다. TNF-α는 Mouse ELISA Ready Set GO kit (eBioscience)로 정량하여 도4에 나타내었다.TNF-α was measured for the anti-inflammatory test of the organic germanium-yeast fermentation of the present invention. Mouse macrophage Raw264.7 cell line was pre-incubated for 24 hours by dispensing 1 × 10 6 cells / well into 12 well cell culture plates. After 24 hours, the medium used for the preculture was removed, and the organic germanium- yeast fermentation product and the yeast fermentation filtrate, and 0.5% aqueous solution of lepagermanium were replaced with the medium added at each concentration of 0, 25, 50, and 100 ppm, followed by 10 Incubated for 1 minute, and incubated for 20 hours by treating LPS at a concentration of 100 ng / ml in the remaining experimental groups except for the normal control group to induce inflammation. Then, the cell culture was used for the experiment by quantifying the protein. TNF-α was quantified with a Mouse ELISA Ready Set GO kit (eBioscience) and is shown in FIG. 4.
상기 실험 결과, 도 4의 결과와 같이, 효모 발효 여과물은 100ng/㎖의 LPS에 의해 분비되는 TNF-α의 양을 50ppm부터 유의하게 감소시켰으며, 0.5% 레파게르마늄 수용액은 100ppm에서 유의하게 LPS에 의해 분비되는 TNF-α의 양을 감소 시켰다. 또한, 유기게르마늄-효모 발효물의 제조물은 25ppm에서도 LPS에 의해 분비되는 TNF-α의 양을 유의하게 감소시켰으며, 이는 50ppm의 효모 발효 여과물과 100ppm의 0.5% 레파게르마늄 수용액보다 향상된 값이다.As a result of the experiment, as shown in Figure 4, the yeast fermentation filtrate significantly reduced the amount of TNF-α secreted by 100ng / ㎖ LPS from 50ppm, 0.5% repagermanium solution was significantly LPS at 100ppm Reduced the amount of TNF-α secreted by. In addition, the preparation of organogermanium-yeast fermentation significantly reduced the amount of TNF-α secreted by LPS even at 25 ppm, which is an improvement over 50 ppm yeast fermentation filtrate and 100 ppm 0.5% repagermanium aqueous solution.
<실험예5. 레파게르마늄-효모발효 여과물의 티로시나아제 억제능>Experimental Example 5. Tyrosinase Inhibition of Lepagermanium-Yeast Fermentation Filtration>
본 발명의 유기게르마늄-효모 발효물의 티로시나아제 억제능을 확인하기 위해티로시나아제의 기질인 tyrosine을 반응시켜 그 억제율을 계산하였다. L-tyrosine을 0.3㎎/㎖의 농도로 인산칼륨완충용액(0.1M,pH6.)에 녹이고, 티로시나아제 역시 인산칼륨완충용액에 1250 U/㎖이 되도록 용해하였다. 유기게르마늄-효모 발효물 및 효모발효 여과물, 0.5% 레파게르마늄 수용액을 각각 50, 100, 500, 2500ppm으로 증류수(대조군)에 농도별로 희석하여 시료로 사용하였고, 150㎕의 시료에 50㎕의 티로시나아제 용액을 첨가하여 37℃에 10분간 방치하였다. 이후, 100㎕의 L-tyrosine 용액을 첨가하여 37℃에 10분 동안 반응시키고, 0℃에서 5분동안 효소반응을 정지시킨 후 475㎚에서 흡광도를 측정하였다. 티로시나아제의 억제율은 하기 수학식 2에 대입하여 계산하였다(표 1).In order to confirm the tyrosinase inhibitory ability of the organic germanium-yeast fermentation of the present invention, the inhibition rate of tyrosine, which is a substrate of tyrosinase, was reacted. L-tyrosine was dissolved in potassium phosphate buffer solution (0.1 M, pH 6.) at a concentration of 0.3 mg / ml, and tyrosinase was also dissolved in potassium phosphate buffer solution to 1250 U / ml. The organic germanium- yeast fermentation product, the yeast fermentation filtrate, and 0.5% aqueous solution of lepagermanium were diluted in distilled water (control) at 50, 100, 500, and 2500 ppm, respectively, and used as a sample. Cinase solution was added and left at 37 ° C for 10 minutes. Thereafter, 100 μl of L-tyrosine solution was added to react at 37 ° C. for 10 minutes, the enzyme reaction was stopped at 0 ° C. for 5 minutes, and the absorbance was measured at 475 nm. The inhibition rate of tyrosinase was calculated by substituting Equation 2 below (Table 1).
[수학식 2][Equation 2]
티로시나아제 억제율(%) = (대조군의 흡광도 - 실험군의 흡광도)/대조군의 흡광도 X 100% Tyrosinase inhibition = (absorbance of control-absorbance of experimental group) / absorbance of
상기 실험 결과, 유기게르마늄-효모 발효물의 IC50값은 467.4ppm으로, 효모발효 여과물의 IC50값 566.2ppm 대비 상향된 효능을 갖는 것으로 확인하였다.As a result of the experiment, the IC 50 value of the organic germanium- yeast fermentation was 467.4ppm, it was confirmed to have an increased potency compared to the IC 50 value 566.2ppm of the yeast fermentation filtrate.
이상에서 본 발명은 기재된 구체예에 대해서만 상세히 설명되었지만 본 발명의 기술사상 범위 내에서 다양한 변형 및 수정이 가능함은 당업자에게 있어서 명백한 것이며, 이러한 변형 및 수정이 첨부된 특허청구범위에 속함은 당연한 것이다. Although the present invention has been described in detail only with respect to the described embodiments, it will be apparent to those skilled in the art that various modifications and changes are possible within the technical scope of the present invention, and such modifications and modifications belong to the appended claims.
(도면 부호 없음)(Not drawing)
Claims (8)
상기 효모균이 사카로마이세스 폼베(Saccharomyces pombe)균이고,
상기 유기게르마늄이 레파게르마늄, 프로파게르마늄 또는 이들의 혼합물임을 특징으로 하는 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물.1 to 10% by weight of rice bran extract, 5 to 30% by weight oat extract, 1 to 3% by weight sugar, 1 to 1% by weight of vitamin B complex aqueous solution, 0.05 to 0.2% by weight yeast extract, and the balance Obtained by adding organogerium, inoculating yeast and incubating,
The yeast is Saccharomyces pombe bacteria,
The organic germanium supplemental yeast fermentation having skin whitening, skin anti-wrinkle and anti-inflammatory effects, characterized in that the organic germanium is lepagermanium, propagernium or a mixture thereof.
(2) 상기 원물 혼합물에 유기게르마늄을 첨가하는 유기게르마늄 첨가단계; 및
(3) 유기게르마늄이 첨가된 원물 혼합물에 효모균을 접종하고, 배양시키는 배양단계;를 포함하고
상기 유기게르마늄이 레파게르마늄, 프로파게르마늄 또는 이들의 혼합물이고,
상기 효모균이 사카로마이세스 폼베(Saccharomyces pombe)균임을 특징으로 하는 피부 미백, 피부 항주름 및 항염증 효능을 갖는 유기게르마늄 보조 효모 발효물의 제조방법.(1) 1 to 10% by weight of rice bran extract, 5 to 30% by weight of oat extract, 1 to 3% by weight of sugar, 1 to 1% by weight of a vitamin B complex aqueous solution, 0.05 to 0.2% by weight of yeast extract and the remaining water A mixing step of preparing a raw mixture;
(2) an organic germanium addition step of adding organic germanium to the raw material mixture; And
(3) inoculating and incubating yeast bacteria in the raw material mixture to which the organic germanium is added;
The organic germanium is lepagermanium, propagernium or mixtures thereof,
The yeast is Saccharomyces pombe ( Saccharomyces pombe ) characterized in that the organic germanium supplemental yeast fermentation product having a skin whitening, anti-wrinkle and anti-inflammatory effect.
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