KR20190125653A - Organic germanium assisted yeast fermentated material having skin whitening, skin antiwrinkle and antiinflammation effect - Google Patents

Abstract

The present invention relates to organic germanium-assisted yeast fermentation having improved skin whitening, skin anti-wrinkle and anti-inflammatory efficacy by adding organic germanium during yeast fermentation of rice bran extract and oat extract; and a manufacturing method thereof. The organic germanium-assisted yeast fermentation having improved skin whitening, skin anti-wrinkle and anti-inflammatory efficacy is obtained by adding organic germanium to an original mixture including rice bran extract, oat extract, sugar, aqueous solution of vitamin B complex and yeast extract, inoculating and incubating yeast bacteria.

Description

Organic germanium assisted yeast fermentated material having skin whitening, skin antiwrinkle and antiinflammation effect}

The present invention relates to an organic germanium supplementary yeast fermentation having a skin whitening, anti-wrinkle and anti-inflammatory effect and a method for preparing the same, and in particular, in yeast fermentation of rice bran extract and oat extract, it is improved by fermenting yeast by adding organic germanium. The present invention relates to an organic germanium supplementary yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory effects, and a method for preparing the same.

With the recent increase in living standards, modern people are paying more attention to maintaining healthy skin in addition to maintaining a healthy body. Therefore, interest in enhancing skin beauty and preventing skin aging is increasing.

As the largest organ of the human body, which occupies about 16% of the total body volume, the skin is in direct contact with the external environment and is important to protect the body from many deadly harmful factors such as temperature, humidity, and ultraviolet radiation. Act as a shield. However, as they age, skin cells are damaged due to various contaminants, strong ultraviolet rays, stress, and malnutrition, and cell proliferation is not performed properly, resulting in wrinkles, loss of elasticity, and keratinization.

Skin aging is largely divided into natural aging (or endogenous aging) and external aging (Doris EB, Cosmet. Toiletries, 1996, 111, 31), and natural aging (endogenous aging) is influenced by genetic factors, so artificial regulation On the other hand, external aging is relatively easy to artificially adjust because of environmental factors. Representative external aging factors include ultraviolet light, reactive oxygen species and stress, and the most prominent external aging phenomenon is wrinkle formation (Daniell HW, Ann. Intern. Med., 1971, 75 (6), 873; Grove GL et al., J. Am. Acad. Dermatol., 1989, 21 (3), 631; Griffiths CE et al., Arch. Dermatol., 1992, 128, 347). As a result, skin elasticity is significantly reduced.

Various studies have been conducted to investigate the mechanism of aging of the skin, revealing various causes of skin aging. When the skin is dried by a change in temperature, a decrease in humidity or wind, the skin physiological activity as a protective barrier against the outside is lowered to promote aging. In addition, exposure of the skin to harmful reactive oxygen species or free radicals causes oxidative action in the body to produce lipid peroxide and cause modification of the various proteins that make up the skin. One of the photoaging mechanisms caused by ultraviolet light is via free radical pathways (Harman D, J. Gerontol., 1956, 11 (3), 298). Free radicals are known to cause breakdown of connective tissue formation, such as cutaneous collagen, cell membrane dysfunction, facilitating DNA mutations, altering protein function, intercellular energy transfer, and metabolism related molecules (Lavker RM and Kligman AM, J. Invest.Drmatol., 1988, 90, 325). The fact that free radicals are involved in aging means that antioxidants or various chemical scavengers that inactivate the free radicals can be used to delay the aging process.

In addition, collagen and elastine related to the elasticity of the skin form a network structure in the dermal tissue of the skin. As the network structure is broken, that is, elastin is decomposed by elastin degrading enzyme (elastase). And wrinkling causes endogenous skin aging. Therefore, skin aging can be suppressed by lowering the activity of elastinase, one of the main causes of skin aging.

Conventionally, it has been relied on cosmetics that are almost applied to the skin surface to prevent skin aging. Korean Unexamined Patent Publication No. 2001-65755 discloses a beta-1,3-glucan and ginseng saponin-containing external preparation composition having skin wrinkle improvement and elasticity enhancing effects such as skin cell proliferation and collagen fiber synthesis. No. 423,521 discloses a cosmetic composition containing ginseng polysaccharides that exhibits an anti-wrinkle effect, a collagen synthesizing effect, a damaged skin recovery effect by optical contact, and a moisturizing effect. However, the external preparations and cosmetics applied directly to the skin surface are not absorbed up to the dermal layer, and act only on the epidermis, so that the skin aging prevention effect is temporary, and there is a problem that the skin is damaged as a side effect. In addition, the active oxygen in the body can not be removed, there was a limit to prevent the aging action by the active oxygen.

In addition, the cosmetic food compositions made of chemicals have a lot of side effects that cause a lot of discontinuation, the situation is urgently required to develop a highly stable skin aging prevention and improvement material even when using for a long time using natural materials. to be.

Republic of Korea Patent Registration No. 10-1187227 (name of the invention: the buttercup beverage using lactic acid bacteria and yeast double fermentation method and its manufacturing method) is "the present invention is a poly to help health through the double fermentation using lactic acid bacteria and yeast The present invention relates to a buttercup beverage using a novel fermentation method of lactic acid bacteria and yeast using a buttercup containing a phenolic component, and to a method of manufacturing the same. The primary fermentation is carried out, and the fermentation of the finished fermentation broth is inoculated with yeast to obtain the fermented buttercup beverage. ”

Republic of Korea Patent Registration No. 10-1177915 (name of the invention: skin anti-aging composition for wrinkle prevention and anti-aging containing yeast fermentation of spinach extract) "The present invention is a spinach extract obtained by inoculating and incubating The present invention relates to an anti-wrinkle and anti-aging skin external composition comprising a yeast fermentation solution as an active ingredient The yeast fermentation solution of the spinach extract has an effect of promoting the synthesis of procollagen and inhibiting the production of collagenase. It is. "

Republic of Korea Patent Registration No. 10-1824179 (name of the invention: moisturizing and anti-inflammatory cosmetic composition comprising heat keeper yeast fermentation) "The present invention includes a heat keeper yeast fermentation having excellent moisturizing and anti-inflammatory efficacy The present invention relates to a cosmetic composition and a method of manufacturing the same, and more specifically, by including a fermented product obtained by fermenting a heat scavenger as an active ingredient in the cosmetic composition, skin irritation is reduced, and an increased moisturizing effect, an antioxidant effect, and an anti-fermentation effect than before fermentation. It has an inflammatory effect. "

The present invention is an organic germanium supplement having improved skin whitening, skin anti-wrinkle and anti-inflammatory effects by yeast fermentation by adding organic germanium at the time of yeast fermentation of rice bran extract and oat extract. It is an object to provide a yeast fermented product and a method for producing the same.

The organic germanium auxiliary yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory effects according to the present invention is added to the organic mixture of the source mixture comprising rice bran extract, oat extract, sugar, vitamin B complex aqueous solution and yeast extract, Obtained by inoculating and culturing yeast.

The yeast is Saccharomyces sp . ), Debaryomyces sp . And Aureobasidum sp . Yeast selected from the group consisting of

The yeast is Saccharomyces Saccharomyces pombe ) can be uniform.

The organic germanium may be lepagermanium, propagernium or a mixture thereof.

The amount of the organic germanium added to the raw extract may be in the range of 0.1 to 10% by weight based on the total weight of the raw extract.

The organic-germanium auxiliary yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory effects according to the present invention comprises the organic germanium auxiliary yeast fermentation having the skin whitening, skin anti-wrinkle and anti-inflammatory effects as an active ingredient. .

The health functional food containing organic germanium supplementary yeast fermentation having skin whitening, skin anti-wrinkle and anti-inflammatory effects according to the present invention is an organic germanium supplementary yeast fermentation having the skin whitening, skin anti-wrinkle and anti-inflammatory effect as an active ingredient Include.

According to the present invention, a method for preparing an organic germanium supplementary yeast fermentation product having skin whitening, anti-wrinkle and anti-inflammatory effects is prepared by (1) preparing a raw mixture including rice bran extract, oat extract, sugar, aqueous solution of vitamin B complex and yeast extract Mixing step; (2) an organic germanium addition step of adding organic germanium to the raw material mixture; And (3) incubating the inoculated yeast with the organic germanium-added raw material mixture and culturing.

According to the present invention, in yeast fermentation of rice bran extract and oat extract, organic whitening having skin whitening, skin anti-wrinkle and anti-inflammatory effects by adding organic germanium to yeast fermentation, and having organic anti-wrinkle and anti-inflammatory effects There is an effect of providing a germanium auxiliary yeast fermentation and a method for producing the same, which can be used as a raw material such as cosmetics.

1 is a fibroblast CCD986-sk cell line (A) for experimenting with the cytotoxicity of the filtrate of the organic germanium auxiliary yeast fermentation obtained in Example 1 of the present invention (hereinafter referred to as "organic germanium- yeast fermentation") Macrophage Raw264.7 cell line (B) is a diagram showing the results of MTT assay by treating the organic germanium- yeast fermentation by concentration.
Figure 2 is an organic germanium- yeast fermentation product (A) of the present invention and the yeast fermentation filtrate (B: the filtrate of yeast fermentation without the accompanying organic germanium), progear of aqueous solution of lepagermanium (C) as organic germanium In order to compare the collagen synthesis ability, it is a figure showing the results of procollagen measurement by treating the organic germanium- yeast fermentation by concentration in the Fibroblast CCD986-sk cell line.
Figure 3 is an organic germanium- yeast fermentation product (A) of the present invention, yeast fermentation filtrate (B), organic germanium to melanoma B16F10 cell line to inhibit the melanin production inhibition of aqueous solution (C) as organic germanium Figure showing the results of quantifying the melanin produced by treating the yeast fermentation by concentration.
Figure 4 is an organic germanium- yeast fermentation product (A) of the present invention and the yeast fermentation filtrate (B), in order to compare the anti-inflammatory effect of the aqueous solution of lepagermanium (C) as organic germanium, organic to Macrophage Raw264.7 cell line It is a figure which shows the result of measuring the secretion amount of TNF- (alpha) by processing germanium yeast fermentation by concentration.
Figure 5 is in vitro tyrosinase activity to compare the tyrosinase inhibition rate of the organic germanium- yeast fermentation product (A) of the present invention and the yeast fermentation filtrate (B), the aqueous solution of lepagermanium (C) as organic germanium, A diagram showing the results of the inhibition test.

Hereinafter, the present invention will be described in detail with reference to specific examples.

The organic germanium supplemental yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory effects according to the present invention is added to the germanium extract to the raw material mixture including rice bran extract, oat extract, sugar, vitamin B complex aqueous solution and yeast extract, It is characterized in that obtained by inoculating and culturing yeast.

The yeast is Saccharomyces sp . ), Debaryomyces sp . And Aureobasidum sp . It may be a yeast selected from the group consisting of, preferably the yeast may be Saccharomyces pombe ( Saccharomyces pombe ) uniform.

The organic germanium may be lepagermanium, propagernium or a mixture thereof. The general efficacy known for organogerium is as follows.

1. Oxygen supply

Numerous nutrients and oxygen are necessary for the human cells to continue metabolism repeatedly, and when oxygen lacks, diseases such as chronic carbon monoxide poisoning, anemia, vascular disorders, heart disorders, hypotension, cell aging, and mental disorders occur. In recent years, medical experiments have shown that germanium acts as an oxygen catalyst to help the efficient use of oxygen. In other words, when the organic germanium is supplied to the human body, it is known that extra oxygen supplies vitality to the blood, thereby releasing chronic oxygen deficiency.

2. Semiconductor effect (regulation of current flow in body cells): Anion effect

The greatest feature of germanium is the semiconductor, which is an electrical property, which has the intermediate property between metal and nonmetal, and is called a miracle element because this property of germanium is the principle that heals all kinds of diseases of the human body. If a foreign body is created in the body, the outer electrons of the four electrons become (-) state and stick out, and the other three are positively charged state in harmony with the body. This role of germanium is the principle that heals diseases of the body, and the acupuncture, moxibustion, and acupressure of the meridians and acupuncture points in the same medicine is the same principle that treats diseases by smoothing the flow of semiconductor cells.

3. strengthening immunity

The human body has two kinds of immune system. First, it is caused by white blood cells, and it is a system that eats pathogens that enter the body by phagocytosis. Second, it is caused by antibodies. B-lymphocytes are formed by T-lymphocytes. It is a system that works by combining pathogens and killing them. Therefore, having cancer means that the number of T-lymphocytes has decreased, and germanium proliferates T-lymphocytes to protect the body from cancer cells, toxic substances, and viruses. Dr. Sato of the Tokyo Institute of Antibacterial and Bacterial Diseases at the University of Tokyo, Japan, also said that organic germanium is one of B.R.M (bioprotective device activating substances) in the clinical trials of organic germanium (Ge-132).

In other words, it is known that organic germanium has a positive effect on the living body, and in the present invention, it is believed that the use of such organic germanium promotes fermentation of yeast and the production of effective bioactive substances.

The raw material mixture may further include a vitamin B complex aqueous solution containing 0.1 to 10% by weight of vitamin B1, 0.01 to 1% by weight of vitamin B2, and 0.1 to 10% by weight of vitamin B2 and vitamin B3 and the balance.

The rice bran extract and oat extract may be hot water extracted rice bran and oats. To prepare the rice bran extract and the oat extract, washing and drying the rice bran and oats, respectively, may be performed by hot water extraction for 2 to 6 hours at 25 to 100 ℃ with distilled water of 2 to 10 times the weight of the sample.

The sugar may be honey.

The raw material mixture contains 1 to 10% by weight of rice bran extract, 5 to 30% by weight of oat extract, 1 to 3% by weight of sugar, 1 to 1% by weight of a vitamin B complex aqueous solution, 0.05 to 0.2% by weight of yeast extract and the balance as water. It can be done by.

The amount of the organic germanium added to the raw extract may be in the range of 0.1 to 10% by weight based on the total weight of the raw extract.

Saccharomyces on the Raw Material Extracts pombe ) was inoculated with Saccharomyces pombe on YM medium and expanded until absorbance values of 600 nm and 1.0 were obtained. The culture may be in the range of 5 to 20% by weight.

The organic-germanium auxiliary yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory effects according to the present invention comprises the organic germanium auxiliary yeast fermentation having the skin whitening, skin anti-wrinkle and anti-inflammatory effects as an active ingredient. .

The health functional food containing organic germanium supplementary yeast fermentation having skin whitening, skin anti-wrinkle and anti-inflammatory effects according to the present invention is an organic germanium supplementary yeast fermentation having the skin whitening, skin anti-wrinkle and anti-inflammatory effect as an active ingredient Include.

According to the present invention, a method for preparing an organic germanium supplementary yeast fermentation product having skin whitening, anti-wrinkle and anti-inflammatory effects is prepared by (1) preparing a raw mixture including rice bran extract, oat extract, sugar, aqueous solution of vitamin B complex and yeast extract Mixing step; (2) an organic germanium addition step of adding organic germanium to the raw material mixture; And (3) incubating the inoculated yeast with the organic germanium-added raw material mixture and culturing.

Hereinafter, preferred embodiments and comparative examples of the present invention will be described.

The following examples are intended to illustrate the invention and should not be understood as limiting the scope of the invention.

Example 1 Preparation of Organic Germanium-Yeast Fermentation

To prepare rice bran extract and oat extract, rice bran and oats were washed and dried, respectively, and hot water extracted for 4 hours at room temperature (20 to 30 ° C.) with distilled water of 4 times the weight of the sample. Then, each extract was centrifuged at 10,000 rpm for 20 minutes to take a supernatant, which was used by filtration. The vitamin B complex aqueous solution was mixed with 5 wt% vitamin B 1, 1 wt% vitamin B 2, 5 wt% vitamin B 3 and water as a balance. Finally, 5% by weight of rice bran extract, 20% by weight oat extract, 5% by weight aqueous solution of vitamin B complex, 2% by weight honey, 0.1% by weight yeast extract, 5% by weight repagermanium, 10% by weight Saccharomyces pombe ) was combined with the culture medium, fermented for 4 days at 37 ℃, the supernatant obtained by centrifugation for 10 minutes at 10,000rpm was filtered to prepare an organic germanium- yeast fermentation.

Experimental Example 1. Cytotoxicity of Organic Germanium-Yeast Fermentation>

To determine the cytotoxicity of organo germanium-yeast fermentation on Fibroblast cells and macrophage cells, MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide) reagent was used. Fibroblast cells and macrophage cells (2X10 ⁴ cells) were added to the organic germanium- yeast fermentation to 0, 50, 100, 250ppm each and incubated at 37 ℃ for 24 hours. After adding the MTT solution to a final concentration of 1 mg / ml and incubating for 4 hours, the medium was removed, the pigment was dissolved in dimethyl sulfoxide (DMSO), and the absorbance was measured at 570 nm. Cell viability was calculated by substituting Equation 1 below.

[Equation 1]

% Cell viability = (absorbance of experimental group / absorbance of control group) X 100

As a result of the experiment, as shown in Figure 1, the organic germanium- yeast fermentation was found to be cytotoxic at the corresponding concentration.

Experimental Example 2. Procollagen Synthesis of Organic Germanium-Yeast Fermented Filtrate>

To test the procollagen synthesis ability of the organic germanium- yeast fermentation of the present invention, the procollagen type I C-Peptide (PIP) EIA Kit (TAKARA) was used to measure the synthesis ability of procollagen. Human fibroblast CCD986-sk cell line was pre-incubated for 24 hours at 5X10 ⁴ cells / well in a 48 well dish, followed by 0, 50, 100, 250 ppm of organic germanium-yeast fermentation and yeast fermentation-free yeast fermentation filtrate. , 0.5% aqueous solution of lepagermanium was treated for 24 hours, at which time 100ng / ml TGFβ was used as a positive control. Then, cells were lysed using PBS containing 0.5% Triton X-100, and the amount of procollagen was measured by procollagen Type I C-Peptide (PIP) EIA Kit, and is shown in FIG. 2.

As a result of the experiment, as shown in Figure 2, the production of the organic germanium- yeast fermentation product and yeast fermentation filtrate promoted procollagen biosynthesis up to about 1.5 times at 250ppm, 0.5% aqueous solution of lepagermanium affects procollagen synthesis capacity Did not give.

Experimental Example 3. Inhibition of Melanin Synthesis of Lepagermanium-Yeast Fermented Filtrate>

In order to determine the melanin synthesis inhibitory ability of the organic germanium- yeast fermentation of the present invention was measured melanin in the cell. Melanoma B16F10 cell line was aliquoted into 6-well plates containing 2 × 10 ⁵ cells / well and incubated 24 hours ago. After incubation, the medium was removed, and 0, 50, 100, and 250 ppm of α-MSH 100nM, an organic germanium- yeast fermentation product, a yeast fermentation filtrate, and a 0.5% aqueous solution of lepagermanium were treated in Phenol red free DMEM medium. After incubation for 96 hours, the medium was removed, washed with PBS, treated with Trypsin-EDTA, and centrifuged at 2000 rpm for 2 minutes to obtain cells. 200 ml of 1N NaOH containing 10% DMSO was added to the cells, followed by stirring. The cells were reacted at 80 ° C for 1 hour, and then transferred to a 96-well plate in 100 ml portions, and the absorbance was measured at 405 nm. The above solution is shown in Figure 3 by comparing the melanin value per protein by protein quantification.

As a result of the experiment, as shown in Figure 3, the organic germanium- yeast fermentation reduced the amount of melanin produced by 100nM α-MSH concentration-dependently, 0.5% aqueous solution of lepagermanium significantly at α-MSH at 250ppm Reduced the amount of melanin produced by In addition, organic germanium-yeast fermentation reduced the amount of melanin produced by α-MSH at 50 ppm to control levels, which was better than 250 ppm of yeast fermentation filtrate (not treated with organic germanium).

Experimental Example 4. Anti-inflammatory Activity of Repagermanium-Yeast Fermented Filtrate>

TNF-α was measured for the anti-inflammatory test of the organic germanium-yeast fermentation of the present invention. Mouse macrophage Raw264.7 cell line was pre-incubated for 24 hours by dispensing 1 × 10 ⁶ cells / well into 12 well cell culture plates. After 24 hours, the medium used for the preculture was removed, and the organic germanium- yeast fermentation product and the yeast fermentation filtrate, and 0.5% aqueous solution of lepagermanium were replaced with the medium added at each concentration of 0, 25, 50, and 100 ppm, followed by 10 Incubated for 1 minute, and incubated for 20 hours by treating LPS at a concentration of 100 ng / ml in the remaining test sample except the normal control group to induce inflammation. Subsequently, the cell cultures of the cells were quantified and used for experiments. TNF-α was quantified with a Mouse ELISA Ready Set GO kit (eBioscience) and is shown in FIG. 4.

As a result of the experiment, as shown in Figure 4, the yeast fermentation filtrate significantly reduced the amount of TNF-α secreted by 100ng / ㎖ LPS from 50ppm, 0.5% aqueous solution of lepagermanium significantly LPS at 100ppm Reduced the amount of TNF-α secreted by In addition, the preparation of organogermanium-yeast fermentation significantly reduced the amount of TNF-α secreted by LPS even at 25 ppm, which is an improvement over 50 ppm yeast fermentation filtrate and 100 ppm 0.5% repagermanium aqueous solution.

Experimental Example 5. Tyrosinase Inhibition of Lepagermanium-Yeast Fermentation Filtration>

In order to confirm the tyrosinase inhibitory ability of the organic germanium-yeast fermentation of the present invention, the inhibition rate of tyrosine, which is a substrate of tyrosinase, was reacted. L-tyrosine was dissolved in potassium phosphate buffer solution (0.1 M, pH 6.) at a concentration of 0.3 mg / ml, and tyrosinase was also dissolved in potassium phosphate buffer solution to 1250 U / ml. The organic germanium- yeast fermentation product, yeast fermentation filtrate, and 0.5% aqueous solution of lepagermanium were diluted in distilled water (control) at 50, 100, 500, and 2500 ppm, respectively, and used as samples. Cinase solution was added and left at 37 ° C for 10 minutes. Thereafter, 100 μl of L-tyrosine solution was added to react at 37 ° C. for 10 minutes, the enzyme reaction was stopped at 0 ° C. for 5 minutes, and the absorbance was measured at 475 nm. The inhibition rate of tyrosinase was calculated by substituting Equation 2 below (Table 1).

[Equation 2]

% Tyrosinase inhibition = (absorbance of control-absorbance of experimental group) / absorbance of control group X 100

As a result of the experiment, the IC ₅₀ value of the organic germanium- yeast fermentation was 467.4ppm, it was confirmed that the yeast fermentation filtrate has an increased efficacy compared to 566.2ppm IC ₅₀ value.

Lepagermanium-Yeast Fermentation Filtrate Yeast Fermentation Filtrate 0.5% germanium solution Concentration (ppm) Inhibition Rate (%) Concentration (ppm) Inhibition Rate (%) Concentration (ppm) Inhibition Rate (%) 50 19.6 ± 0.6 50 19.2 ± 0.3 50 2.7 ± 0.2 100 23.6 ± 0.3 100 21.1 ± 0.7 100 3.0 ± 0.3 500 52.4 ± 0.8 500 47.4 ± 0.5 500 7.4 ± 0.2 2500 97.8 ± 0.3 2500 98.2 ± 0.6 2500 12.2 ± 0.3 * IC ₅₀ = 467.4 * IC ₅₀ = 566.2

Although the present invention has been described in detail only with respect to the described embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible within the technical scope of the present invention, and such modifications and modifications are within the scope of the appended claims.

(Not drawing)

Claims (8)

Skin whitening, anti-wrinkle and anti-inflammatory effects, characterized in that obtained by adding organic germanium, inoculating and incubating yeast bacteria to an original mixture comprising rice bran extract, oat extract, sugar, aqueous vitamin B complex and yeast extract Organic Germanium Supplementary Yeast Fermentation. The method of claim 1, wherein the yeast is Saccharomyces sp . , Debaryomyces sp . ) And Aureobasidum sp . Organic germanium supplementary yeast fermentation having skin whitening, skin anti-wrinkle and anti-inflammatory effect, characterized in that it is a yeast selected from the group consisting of. The organic germanium supplemental yeast fermentation product according to claim 2, wherein the yeast is Saccharomyces pombe , which has a skin whitening, anti-wrinkle and anti-inflammatory effect. 2. The organic germanium supplemental yeast fermentation according to claim 1, wherein the organo germanium is lepagermanium, propagermanium or a mixture thereof. According to claim 1, Organic germanium supplement having skin whitening, skin anti-wrinkle and anti-inflammatory effect, characterized in that the addition amount of the organic germanium to the raw extract is within the range of 0.1 to 10% by weight based on the total weight of the raw extract. Yeast Fermentation. Skin whitening, skin anti-wrinkle and anti-inflammatory, characterized in that it comprises an organic germanium auxiliary yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory efficacy according to any one of claims 1 to 5 as an active ingredient. Cosmetic containing organic germanium supplementary yeast fermentation having efficacy. Skin whitening, skin anti-wrinkle and anti-inflammatory, characterized in that it comprises an organic germanium auxiliary yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory efficacy according to any one of claims 1 to 5 as an active ingredient. Health functional food containing organic germanium supplementary yeast fermented product having efficacy. (1) a mixing step of preparing a raw material mixture including rice bran extract, oat extract, sugar, aqueous vitamin B complex and yeast extract;
(2) an organic germanium addition step of adding organic germanium to the raw material mixture; And
(3) a culture step of inoculating and incubating the yeast bacteria in the raw material mixture to which the organic germanium is added;
Method for producing an organic germanium supplementary yeast fermentation product having skin whitening, skin anti-wrinkle and anti-inflammatory effect, comprising a.

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