KR100658296B1 - A cosmetic composition containing organic germanium for wrinkle reduction - Google Patents

Abstract

The present invention relates to an anti-wrinkle cosmetic composition containing organo germanium which promotes the synthesis of collagen in the dermis, thereby reducing wrinkles and having excellent stability and safety in the formulation.

The present invention can provide an organic germanium-containing anti-wrinkle cosmetic composition that can alleviate wrinkles already produced by internal or external factors without deterioration of skin side effects and wrinkles, and is a very useful invention in the cosmetic industry.

Organic Germanium, Wrinkle Relief, Collagen Synthesis, Cell Proliferation

Description

A cosmetic composition containing organic germanium for wrinkle reduction}

The present invention relates to a cosmetic composition containing organic germanium to relieve wrinkles. More specifically, the present invention relates to an anti-wrinkle cosmetic composition containing organic germanium, which promotes the synthesis of collagen in the dermis to reduce wrinkles and has excellent stability and safety in the formulation.

Human skin is constantly undergoing a change, the most representative of which is deterioration of skin function due to aging and reduction of visual beauty. Therefore, the area of greatest interest and research in cosmetics is how to delay skin aging.

In general, theories explaining skin aging include mutagenesis of somatic cells, error generation of gene replication, gene program theory, free radical theory, autoimmune response theory, toxic substance accumulation theory, and dermal component protein modification theory. Rieger, C & T, 110,94,1994) There are many types, but all of them are involved in skin aging, and free radical theory is known to be a major cause of skin aging. The final appearance of the skin as a result of skin aging is the formation of wrinkles, decreased skin elasticity, the formation of senile spots, the formation of wrinkles is the most typical phenomenon. Free radicals are chemically highly reactive chemicals that occur when the skin is irradiated with UV light or during the respiration of cells in the skin. Most of the free radicals related to aging of the skin are Reactive Oxygen Species (ROS), which include superoxide radical, hydrogen peroxide (H ₂ O ₂ ) and hydroxy radical (Hydroxy radical). ), Singlet oxygen, and the like. These reactive oxygen species cause peroxidation of lipids, generate various active species such as radicals, peroxides, aldehydes and epoxides, and damage DNA, RNA, proteins, cell membranes and cell structures, cancer and tissue damage. It is thought to be the main cause of aging, etc. (Black HS, Photochem photobiol, 46 (2), 213, 1987). However, in spite of many of these studies, it is not yet known how each change is related to the appearance of wrinkles in the skin's appearance, and thus research on the mechanism of wrinkle formation is still ongoing.

Recently, a lot of physiological studies have been conducted on the factors causing wrinkles on the skin. In the cosmetics, the prevention of wrinkles is first achieved by maintaining the moisture of the stratum corneum with a moisturizer, protecting the skin from ultraviolet rays or the environment, and promoting the activation of new cells. Prevention and the like are applied. In addition, materials used to suppress skin aging so far include enzymes such as superoxide dismutase and catalase, and α-tocopherol (vitamin E) and β- Fat-soluble and water-soluble antioxidants or radical scavengers, such as carotene (β-Carotene), ascorbic acid (Vitamin C), and glutathione, are mainly used to prevent the formation of wrinkles due to skin aging. In addition, vitamin A, alpha hydroxy acid and the like, which are recently spotlighted as anti-aging substances, have a healing effect to alleviate wrinkles generated through collagen synthesis promotion in the dermis. In particular, vitamin A and alpha hydroxy acids have a wrinkle-reducing effect faster than antioxidants and additional effects such as skin cell regeneration and whitening have been reported (R. Hermitte, C & T, 107, 63, 1992). However, the antioxidant or radical scavenger, vitamin A, alpha hydroxy acid and the like has a disadvantage in that the stability and safety, etc. when blended in cosmetics.

The present invention is to solve the problems of the prior art as described above, the inventors of the present invention while conducting research on the excellent stability and safety at the same time excellent wrinkle relief effect when formulated in cosmetics (Organic Germanium) It has been found that the anti-wrinkle effect through the dermal collagen promotion and the stability and safety in the formulation are very excellent, and the effect has been proved in human experiments to complete the present invention. Accordingly, an object of the present invention is to provide an anti-wrinkle cosmetic composition containing organo germanium excellent in cell proliferation effect and collagen synthesis enhancement effect.

Organic Germanium was first synthesized in 1968 by Dr. Asai, and is present in small amounts in ginseng, garlic, ganoderma lucidum, goji berries, and yulmu. Organic Germanium has shown some recognized medical effects in Japan, with oral doses of 2 g daily reported partial treatment or cure of viral infections, circulatory diseases, and malignant tumors. K. Asai Miracle cure: Organic Germanium, Japan Publications Inc ISBN 0-87040-1,1980).

Recently, as a result of scientifically exploring its efficacy under better conditions, very effective interferon-induced function, immunomodulatory action, and anti-tumor effect have been reported (M. Tsutui. Et al, J. Am Chem. Soc). 98,8278-8289 (1976), H Aso, et al Microbiol. Immunol. 29,65-74 (1985), F. Suzuki RR Brutkiewicz aud RB Pollard, Anticancer Res. 6,177-183 (1986). Therefore, to date, organic germanium has been researched by various researchers, and there are many patents and reports on its effects, and typical examples are acne alleviating and treating effects, antioxidant effect showing radical scavenging ability, and UV damage. Protective effects, softening skin, improving abnormal pigmentation, and treating atopic skin diseases have been reported. (JP 63183570, JP62111908, US4229468, US4889715, US4623667, K.Asai Miracle cure: Organic Germanium, Japan Publications Inc ISBN 0-87040-1,1980). However, there have been no patents or reports on the anti-wrinkle effect by promoting collagen synthesis, which is an effect claimed in the present invention, and thus the present inventors could finally complete the present invention through a demonstration experiment on the anti-wrinkle effect. .

The organic germanium added to the present invention used was commercially available under the trade name Arlamol GEO from ICI, UK, and the chemical name of the organic germanium is biscarboxyethyl germanium sesquioxide (Biscarboxyethyl germanium). sesquioxide) and its structural formula is (GeCH ₂ CH ₂ C00H) ₂ O ₃ , and has the following structure.

The present invention is to add an organic germanium to cosmetics, that is, soft cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, packs and the like. In more detail, the organic germanium is added to the aforementioned cosmetics in an amount of 0.001 to 2% by weight, preferably 0.01 to 1% by weight, based on the dry weight. However, it is difficult to expect a substantial effect when the organic germanium is added at 0.001% by weight or less, when it is added at 2% by weight or more, its dissolution and solubilization is difficult, and phase separation easily occurs, resulting in poor stability of the product over time. The effect was not increased.

Hereinafter, the specific configuration and operation of the present invention will be described in more detail through experimental examples, examples and comparative examples. However, the present invention is not limited to the following examples.

Experimental Example 1: Cell Proliferation Effect

The cell activity of the organic germanium was measured by the effect of cell proliferation. Cell proliferation was first measured by inoculating human fibroblasts into a 96 well plate (96 well plate, corning) three days later, by quantifying the total protein of each cell, cell activity was measured. Serum (10%) was added as a control and serum-free medium was used as a comparative group. The results are shown in Table 1 below.

Cell proliferation effect Type of sample % Cell proliferation after 3 days Control group (Serum 10%) 242 2% organic germanium 225 1% organic germanium 210 Organic Germanium 0.1% 170 Organic Germanium 0.01% 130 Organic Germanium 0.001% 110 Organic Germanium 0.0001% 102

As shown in Table 1, it can be seen that the organic germanium has a very large effect on increasing cell activity through cell proliferation.

Experimental Example 2: Collagen Synthesis Enhancement Effect

As described above, collagen synthesis enhancement effects were measured using cells cultured with organic germanium having excellent cell activity enhancing ability as follows. First, the human fibroblasts were incubated for 24 hours in a 24 well plate and then reacted with L-2,3- ³ H proline for 24 hours. At this time, organic germanium was added to the positive control group by concentration, and nothing was treated to the negative control group. After the reaction of L-2,3- ³ H proline, the amount of neonatal collagen synthesized by fibroblasts in the cell medium was measured and compared using collagen degrading enzyme and radioisotope activity analyzer. Serum (10%) was added as a control and serum-free medium was used as a comparison group. The results are shown in Table 2 below.

Collagen Synthesis Enhancement Effect  Type of sample Collagen Synthesis Enhancement Effect (%) Control group (Serum 10%) 360 2% organic germanium 325 1% organic germanium 320 Organic Germanium 0.1% 250 Organic Germanium 0.01% 190 Organic Germanium 0.001% 150 Organic Germanium 0.0001% 105

As shown in Table 2, it can be seen that the organic germanium has an excellent synergistic effect of collagen synthesis at the cellular level. At 1% or more, there was almost no synergistic effect of collagen synthesis, and it was difficult to recognize the effect at 0.0001% or less. As described above, an organic germanium having an excellent cell proliferation effect and collagen synthesis enhancement effect was performed as follows.

Examples 1 to 3 and Comparative Example 1

According to the composition of Table 3, Examples 1 to 3 were prepared by combining the organic germanium in a general manner, Comparative Example 1 was prepared an oil-in-water emulsion according to the following method with a comparative sample that does not contain it. First, the aqueous phase and the oil phase were heated and dissolved at 70 to 75 degrees, respectively, and then the oil phase was added to the aqueous phase, emulsified under a homomixer under 6000 rpm for 10 minutes, and cooled to 30 ° C to prepare an oil-in-water emulsion.

Wrinkle Relief Cosmetic Composition Example 1 Example 2 Example 3 Comparative Example 1 Awards Purified water Remaining amount Remaining amount Remaining amount Remaining amount Triethanolamine 0.15 0.15 0.15 0.15 Carboxy vinyl polymer (1 wt% solution) 15 15 15 15 Dark glycerin 6 6 6 6 Propylene glycol 4 4 4 4 Paraoxybenzoic acid ester 0.2 0.2 0.2 0.2 Organic germanium 0.01 0.1 1.0 - Paid Cetostearyl alcohol One One One One Glyceryl Monostearate One One One One Sorbitan monosulfate One One One One POE (20) sorbitan monostearate 1.5 1.5 1.5 1.5 Paraoxybenzoic Acid Profiles 0.1 0.1 0.1 0.1 Isocetil Ministate 5 5 5 5 Isocetyloctanoate 5 5 5 5 Squalane 5 5 5 5 Spices Quantity Quantity Quantity Quantity

The unit of the numerical value used in Table 3 is weight%. In addition, as a carboxyvinyl polymer, carbopol 941 (B.F.Goodrich), USA, was used as a 1% by weight aqueous solution. In addition, monostearate sorbitan was used as a product sold under the trade name Alasel 60 (ICA), UK, and isocetyl octanoate was used as a product of Nihon kokyu alcohol (Nihon kokyu alcohol) ICEH was used.

Experimental Example 3 Relief of Fine Lines

In order to evaluate the anti-wrinkle effect of the products prepared according to Examples 1 to 3 and Comparative Example 1, the sample was uniformly applied twice a day to the eyes around 60 women from 40 to 60 years old. The site was defined as crow's feet for 3 months. The test subjects were divided into three groups, and each of 20 subjects applied Examples 1 to 3 containing organic germanium at the left eye, and Comparative Example 1 containing no organic germanium at the right eye.

After 3 months, the wrinkles were relieved and the depth of the wrinkles was measured by visual analysis of the eye wrinkle template (replica) before and after visual judgment and sample application. The visual judgment criteria were determined by four stages of no improvement, slight improvement, moderate improvement, and significant improvement compared to the respective base coating controls, and the results are shown as the number of people in Table 4 below. The results are shown in Table 5 below as the depth of wrinkles (um).

Reduction of fine wrinkles by visual judgment No improvement Minor improvements Moderate improvement A significant improvement commandment) Example 1 8 6 4 2 20 Example 2 4 4 8 4 20 Example 3 2 3 8 7 20

As can be seen in the results of the fine wrinkles by the visual determination of Table 4, the examples according to the present invention shows the effect of alleviating wrinkles in all cases containing organic germanium, in particular Example 3 is 80% It showed a significant wrinkle relief effect that can be recognized abnormal.

Wrinkle depth measurement result by image analysis Sample application (um) After sample application (um) Wrinkle depth reduction rate (%) Comparative Example 1 315 300 5.0 Example 1 308 279 9.4 Example 2 290 250 13.8 Example 3 295 240 18.6

As can be seen from the result of measuring the depth of the wrinkles by the image analysis of Table 5, the depth of the wrinkles is reduced by 5.0% in Comparative Example 1, while Examples 1 to 3 are 9.4 ~ 18.6 depending on the organic germanium concentration % Was reduced to show an effective effect of reducing the depth of wrinkles compared to Comparative Example 1.

In view of the above-mentioned enhancement of collagen synthesis at the skin cell level (Experimental Example 2) and the reduction of the depth of the eye wrinkles (Experimental Example 3), the organo germanium-containing cosmetics showed an effective relieving effect on the wrinkles already produced. No skin side effects and worsening of wrinkles were observed.

As described above and can be seen through the experimental examples and examples, the organic germanium-containing anti-wrinkle cosmetic composition of the present invention increases the cellular activity through cell proliferation and relieves wrinkles generated through the enhancement of collagen synthesis in the dermis. The effect is excellent. Therefore, the present invention has an effect that can alleviate the wrinkles already generated by internal or external factors without deterioration of skin side effects and wrinkles.                     

Claims (2)

Wrinkle relief cosmetic composition characterized in that it contains 0.001 to 2% by weight of organic germanium (Organic Germanium) having the following structural formula in dry weight.

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