KR20150078868A - Cosmetic composition containing ginsenoside Rd, Rb2 and Bambusae Caulis in Taeniam extracts - Google Patents

Abstract

The present invention relates to an anti-wrinkle and skin moisturizing cosmetic composition containing ginsenoside Rd and Rb2, and a Bambusae caulis in Taeniam extract as active ingredients. If a mixture of ginsenoside Rd and Rb2 is mixed with the Bambusae caulis in Taeniam extract and then used, a synergistic effect is created and accordingly, provided are effects of proliferating an excellent fibroblast cell, promoting biosynthesis of collagen, inhibiting MMP-1 generation, inhibiting activity against elastase, increasing a skin moisture content, thereby exhibiting complex effects of reducing and ameliorating wrinkles and improving skin moisturizing. Accordingly, the invention can be usefully used as an anti-wrinkle and skin moisturizing cosmetic composition.

Description

(Cosmetic composition containing ginsenoside Rd, Rb2 and Bambusae Caulis in Taeniam extracts containing an active ingredient of ginsenosides Rd, Rb2,

The present invention relates to a cosmetic composition for improving wrinkles and skin moisturizing comprising ginseng-derived component ginsenoside and kiss extract as an active ingredient, and more particularly to a cosmetic composition for ginsenoside Rd, Rb2 and kiss extract, The present invention also relates to a cosmetic composition having the above-mentioned effect.

Skin aging is divided into endogenous aging by physiological aging and exogenous aging by continuous UV exposure. Endogenous aging decreases fibroblast action and cell number with age, reduces the amount of synthesis of extracellular matrix protein fibers such as collagen, elastin and fibrillin, loosens the structure, decreases elasticity, The structure of the stratum corneum varies. Exogenous aging is caused by the activation of activator protein-1 (AP-1) by activation of various signal transduction systems by external reactive factors such as ultraviolet rays, causing reactive oxygen species (ROS) Lipids, proteins, nucleic acids, enzymes, etc. are damaged and aging occurs. Aging is caused by structural changes of the extracellular matrix in the dermal layer of the skin. The representative symptom of the component is the occurrence of fine lines and wrinkles caused by the reduction in elasticity, which can be attributed to a marked reduction in the collagen content of the skin elastic component protein in the collagen of the dermal tissue of the skin.

In addition, the horny layer of the skin contains a sebum component and a natural moisturizing factor to make the skin always moist. When the skin becomes old or dry, such natural moisturizing factors can not maintain sufficient moisture in the stratum corneum. You must. In particular, it prevents the transepidermal water loss of the damaged skin layer by moisturizing agent or moisturizes the rough skin by supplying moisture, and fills the irregular space created by the peeled corneocytes, thereby increasing the flexibility of the skin .

In recent years, cosmetic compositions using ginsenoside components of ginseng, which are excellent in moisturizing effect by restoring the skin's inherent functions from skin aging and activating skin cells to suppress skin wrinkle formation, have been developed. Korean Patent Laid-Open Publication No. 2013-0135222 entitled " Cosmetic Composition for Improving Wrinkles "discloses a cosmetic composition for improving wrinkles containing a fermented extract of red ginseng as an active ingredient. Korean Patent Publication No. 2013-0028456 entitled & A composition for preventing skin aging and improving skin wrinkles containing red ginseng extract and malt extract "includes" Red ginseng extract treated with koji mold-derived enzyme and malt extract, and a pharmaceutical composition for preventing skin aging and improving skin wrinkles " .

The present inventors continued research to achieve maximization of the efficacy by selecting and combining specific ginsenosides from various ginsenosides derived from ginseng. As a result, they found that combinations of ginsenosides Rd and Rb2 in a specific combination ratio When the extract is mixed with the extract, the wrinkle is improved and the moisturizing effect is excellent by the synergistic action. Thus, the present invention has been completed.

Therefore, it is an object of the present invention to provide a cosmetic composition which contains ginsenosides Rd and Rb2 and a killed extract as an active ingredient, thereby improving wrinkles and skin moisturizing effect.

In order to achieve the above object, the present invention provides a cosmetic composition for improving and moisturizing wrinkles containing a mixture of ginsenosides Rd and Rb2 and a killed extract as an active ingredient. In this case, it is preferable that the ginsenosides Rd and Rb2, which are effective components, are mixed in a weight ratio of 2: 1 each of ginsenosides Rd and Rb2. The mixture of the ginsenosides Rd and Rb2 and the killed extract are preferably contained in a weight ratio of 1: 1 to 10, more preferably 1: 5. It is preferable that the mixture of the ginsenosides Rd and Rb2 and the kiss extract as the active ingredient are each contained in an amount of 0.1 to 15% by weight based on the total weight of the cosmetic composition.

The cosmetic composition containing the ginsenosides Rd, Rb2, and Killer extract of the present invention in a specific combination ratio is more excellent in fibroblast proliferation effect, collagen production effect, elastase activity inhibition effect, Water-loss-inhibiting effect, and moisture content-increasing effect.

Hereinafter, the present invention will be described in detail.

The ginsenosides Rd and Rb2 of the present invention can be prepared as follows. For example, a ginseng extract is first prepared by adding a lower alcohol such as ethanol to dried ginseng, extracting it at an extraction temperature of 100 ° C for 2 hours, filtering it, and then evaporating the alcohol. Lactobacillus pectinase is added to ginseng extract and reacted at 20 to 60 ° C for 1 to 72 hours. Aglycon obtained by the reaction is dissolved in an organic solvent and then dried with a vacuum evaporator to obtain a powder corresponding to ginsenosides Rd and Rb2.

The ginsenosides Rd and Rb2 of the present invention were mixed and tested at various ratios. As a result, it was confirmed that the preferred mixing ratio of the cosmetic material for improving wrinkles and moisturizing of the present invention was 2: 1. On the other hand, in the preparation of the ginsenoside mixture of the present invention, a powder in which a high content of Rd and Rb2 are mixed can be obtained from the enzyme reaction of ginseng, or may be mixed after obtaining each ginsenoside.

Bambusae Caulis in Taeniam, which is used as one active ingredient of the present invention, is an intermediate layer obtained by removing the crust of Phylostachys nigra Munro var. Henosis Stapf or Phllostachys bambusoides Sieb. Et Zucc. It says. It is effective for vomiting, hiccups, hiccups, vomiting due to vaginal discharge and vomiting, and for relaxing the fetus. . A strong inhibitory action on E. coli was reported by pharmacological action.

The killing extract used as an active ingredient of the present invention can be obtained according to a conventional method in the art, and the production method thereof is not particularly limited. For example, it is preferable to use a solvent such as water, an aqueous solution of methanol or methanol, an aqueous solution of ethanol or ethanol, acetone, ethyl acetate, 1,3-butylene glycol or 1,3-butylene glycol aqueous solution, hexane, diethyl ether, -Propanol and n-butanol as an extraction solvent at 20 to 80 캜 and concentrating under reduced pressure to obtain a powder or a liquid.

The mixture of the ginsenosides Rd and Rb2, which are effective ingredients of the present invention, and the killed extract are contained in an amount of 0.1 to 15% by weight based on the total weight of the cosmetic composition. If the content is less than 0.1% by weight, the effect is insignificant. If the content is more than 15% by weight, the increase in the effect is not large compared with the content, which is rather inefficient.

The active ingredient of the present invention, ginsenoside Rd, Rb2 mixture and killed extract, are contained in a weight ratio of 1: 1 to 10, more preferably 1: 5, respectively. When the ratio is less than 1: 1 or exceeds 1:10, synergistic effects between the active ingredients are not exhibited.

If the ginsenosides Rd and Rb2 and the killed extract are in the form of a powder or a solution in the form of a nanocapsule, a microcapsule, a nanosome or the like, a person skilled in the art will understand that the concentration of the solution or powder You will be able to control the amount.

The present invention can be manufactured in the form of a general emulsified formulation and a solubilized formulation in the form of a cosmetic composition comprising the ginsenoside Rd, Rb2 mixture and kiss extract as an active ingredient. Cosmetics of emulsified form include nutrition lotion, cream, essence, etc., and cosmetics of solubilized form have softening longevity. In addition, the ginsenosides Rd and Rb2 of the present invention and the kiss extract can be prepared in the form of a topical or systemic adjuvant commonly used in the skin science field by containing a dermatologically acceptable medium or base in addition to cosmetics have.

In addition, the cosmetic composition of the present invention may further comprise, in addition to the mixture of ginsenoside Rd, Rb2 mixture and kiss extract, a lipid, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, an antioxidant, a suspending agent, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents , Lipid vesicles, or any other ingredient commonly used in cosmetics, as well as adjuvants commonly used in the cosmetics or dermatological sciences. And the above ingredients may be introduced in amounts commonly used in the dermatology field.

The cosmetic composition of the present invention can be used as a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, a nutritive cream, a moisturizing cream, a hand cream, , Cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder, eye shadow and the like.

[Example]

Hereinafter, the present invention will be described in more detail based on the following examples and test examples. However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

Manufacturing example  One : Jinse Nosad Rd  Produce

After washing the ginseng with purified water, the ginseng is crushed into small pieces, and ethanol is added to the ginseng 7-8 times its dry weight. The mixture was refluxed at 100 ° C for 2 hours, boiled, and then filtered to obtain an extract. Ethanol was evaporated to obtain ginseng extract. The extract of ginseng extract was dissolved in an aqueous solvent, lactobacillus pectinase derived from microorganism was added thereto, and reacted at 20 to 60 ° C for 1 to 72 hours to obtain aglycon containing ginsenoside Rd. This aglycon was dissolved in an organic solvent, and then the organic solvent was evaporated with a reduced pressure evaporator to prepare ginsenoside Rd.

Manufacturing example  2 : Jinse Nosad Rb2  Produce

After washing the ginseng with purified water, the ginseng is crushed into small pieces, and ethanol is added to the ginseng 7-8 times its dry weight. The mixture was refluxed at 100 ° C for 2 hours, boiled, and then filtered to obtain an extract. Ethanol was evaporated to obtain ginseng extract. The extract of ginseng extract was dissolved in an aqueous solvent, lactobacillus pectinase derived from microorganism was added thereto, and reacted at 20 to 60 ° C for 1 to 72 hours to obtain aglycon containing ginsenoside Rb2. The aglycon was dissolved in an organic solvent, and the organic solvent was evaporated using a vacuum evaporator to prepare ginsenoside Rb2.

Manufacturing example  3: Kill Extract  Produce

After washing with purified water, drying, killing, killing, killing, adding to water 10 times the weight, extracting at 20 ~ 80 ℃ for more than 3 hours and filtering. The extract was concentrated under reduced pressure at 60 DEG C in a distillation apparatus equipped with a cooling condenser to prepare powder or liquid.

1: 1, 2: 1, 3: 1, 5: 1 mixture of the ginsenosides Rd and Rb2 prepared in Preparation Examples 1 and 2 and the ginsenoside mixture and the killing extract were mixed at a ratio of 1: 1: 5, 1:10, and diluted solution prepared by diluting red ginseng extract with dimethylsulfoxide by concentration.

Test Example  One : Elastase  Control active test

10 μl of a solution prepared by mixing test sample and Elastase enzyme solution (1,000 units / mL, Sigma, USA) in 2.5% by weight agar containing 0.1% by weight of Elastin-Congo red (Sigma, USA) and reacting at room temperature for 10 minutes And then cultured at 37 ° C for 18 hours. After the end of the culture, when a zona tangle is formed around the Elastin-Congo red agar due to the activity of the Elastinase, the diameter of the halo can be measured to measure the inhibitory effect of the Elastinase activity.

Gin Senocide Rd , Rb2  Inhibitory activity of the mixture

In order to confirm the elastase inhibitory activity of the above-mentioned ginsenosides Rd and Rb2 and the mixture thereof, the concentration of the elastase was adjusted to 0.01, 0.05, 0.1 and 0.5% of the solution was added dropwise with the elastase to measure the diameter of the halo appearing on the medium to measure the inhibitory activity of the elastase. As a control, retinol crystal was mixed with the elastase Respectively. The results are shown in Table 1 below.

sample
density
(%) Halo diameter (cm) Red ginseng
extract Gins. Rd Gins.
Rb2 Gins. Rd + Rb2 Elastase Retinol
crystal 1: 1 2: 1 3: 1 5: 1 0.01 1.8 1.6 1.8 1.7 1.5 1.6 1.6 1.8 0.9 0.05 1.8 1.5 1.6 1.6 1.3 1.4 1.5 0.10 1.7 1.2 1.6 1.4 1.1 1.1 1.2 0.50 1.5 1.2 1.4 1.3 1.0 1.1 1.1

As shown in Table 1 above, the mixture of ginsenosides Rd and Rb2 exhibited excellent elastase inhibitory activity as compared with the whole extract of red ginseng.

It was found that the halosilane (Rx) and the Rb2 mixture, especially in the weight ratio of 2: 1, showed a remarkable decrease in the halo diameter and showed a superior elastase inhibitory effect. When each of the ginsenosides was treated , It was found that there was a synergistic effect when the mixture was treated with a mixture of ginsenoside Rd and Rb2.

Gin Senocide Rd , Rb2  Mixture Kill the extract of  activation

The 2: 1 mixture of ginsenosides Rd and Rb2, which has been confirmed to have the most excellent inhibitory activity, was killed and the inhibitory activity of elastase according to mixing with the extract was measured. The results are shown in Table 2 below. ≪ tb >< TABLE >

sample
density
(%) Halo diameter (cm) Red ginseng
extract Gins. Rd Gins.
Rb2 Gins. (Rd + Rb2 = 2: 1):
Kill Extract Elastase
Retinol
crystal 1: 1 1: 5 1:10 0.01 1.8 1.6 1.8 1.5 1.5 1.6 1.8 0.9 0.05 1.8 1.5 1.6 1.3 1.2 1.3 0.10 1.7 1.2 1.6 1.1 1.0 1.1 0.50 1.5 1.2 1.4 1.0 0.9 1.0

As shown in the above Table 2, when the mixture of ginsenoside Rd and Rb2 mixture was mixed with killing extract, the inhibition activity was excellent , Especially 1: 5 ratio.

Test Example  2: Collagen biosynthesis promoting effect test

Fibroblasts were inoculated into IMDM medium supplemented with 10% FBS at a cell concentration of 5 × 10 ⁵ cells and cultured in a 5% CO ₂ incubator at 37 ° C. for 24 hours. And cultured for 24 hours. As shown in the following Table 3, red ginseng extract, ginsenoside Rd and Rb2 prepared in Preparation Examples 1 and 2, a mixture thereof, and a mixture prepared by mixing the extract with the extract were mixed at final concentrations of 50, 100 and 500 , Diluted in dimethyl sulfoxide to give a concentration of 1000 μg / ml, and incubated for 18 hours under the same conditions. The cells were then exposed to UV light for 1 hour to stress the cells. After that, fibroblasts were recovered using Trizol reagent (Invitrogen, USA), and mRNA was extracted and cDNA was synthesized through a series of procedures. Gene amplification was carried out using 10x taq polymerase buffer, 10 mM dNTP, 10 mM Tris-HCl buffer (pH 7.0) using a thermal cycler (GenePro, Hangzhou Bioer tech., CHINA). The amplified product was amplified by electrophoresis The PCR reaction was carried out by adding 50 μL of distilled water to 95 ° C for 5 min and 1 cycle / 95 ° C for 1 min / 51 ° C. 2 minutes / 72 degrees 1 minute, 28 cycles amplification and 72 degrees for 5 minutes. The expression rate of the generated procollagen was calculated according to the following equation. As a control, normal fibroblasts without UV irradiation were used.

procollagen expression rate (%) = B / A × 100 (%)

A: The amount of procollagen expression in the control group

B: The amount of procollagen expressed in the sample treated and UV irradiated fibroblasts

As a result of the test, the results shown in Table 3 were obtained.

sample
density
(占 퐂 / ml) procollagen expression rate (%) Red ginseng extract Gins. Rd Gins. Rb2 Gin Senocide Rd + R2 Gins. (Rd + Rb2 = 2: 1): Kill the extract TGF-beta 1: 1 2: 1 3: 1 5: 1 1: 1 1: 5 1:10 0 2.63 2.63 2.63 2.63 2.63 2.63 2.63 2.63 2.63 2.63 2.63 50 13.83 26.52 18.48 28.04 41.20 40.92 35.05 41.35 43.62 41.35 44.15 100 29.57 49.12 35.86 47.25 57.82 53.87 51.66 58.74 59.43 57.97 59.99 500 63.10 83.07 70.21 81.10 96.18 94.05 88.23 97.16 98.75 96.91 99.07 1000 90.99 118.47 102.67 119.03 140.36 138.76 124.14 141.98 143.61 140.96 143.91

As can be seen from the results of Table 3 above, the sample in which the ginsenosides Rd and Rb2 were mixed at a ratio of 2: 1, compared with the case where the ginsenosides Rd and Rb2 were applied alone, was more effective in promoting collagen biosynthesis , And showed synergistic effects. In addition, when the mixture of 2: 1 mixture of ginsenosides Rd and Rb2 was killed and the extracts were mixed in a ratio of 1: 1 to 10, the effect was more excellent and the effect was similar to that of the control TGF-β.

Test Example  3: MMP -1 production inhibitory effect

Human normal skin cells, fibroblasts (Korean Cell Line Bank, Korea), were inoculated into 48-well microplates (Nunc, Denmark) to be 10 < ⁶ > cells per well and cultured in DMEM medium (Sigma, After 24 hours of incubation, the red ginseng extract, the ginsenosides Rd and Rb2 prepared in Preparation Examples 1 and 2, and the mixture thereof, and the mixture of the extract and the mixture of the extracts, were mixed at final concentrations of 50, 100, / Ml, and the cells were cultured in serum-free DMEM medium for 48 hours. After the incubation, the supernatant of each well was collected and the amount (쨉 g / ml) of the newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA) And the results are shown in Table 4 below. At this time, TGF-beta (10 mg / ml, Roche, USA) was used as a positive control for the inhibition rate of MMP-1 production.

sample
density
(占 퐂 / ml) Inhibition rate of MMP-1 production (%) Red ginseng extract Gin Senocide Rd Gin Senocide
Rb2 Gin Senocide Rd + Rb2 Gins (Rd + Rb2 = 2: 1): Kill the extract TGF-beta 1: 1 2: 1 3: 1 5: 1 1: 1 1: 5 1:10 50 9.7 16.9 12.8 14.0 25.4 18.7 17.1 27.6 29.2 26.6 21.4 100 13.4 25.7 18.9 20.9 47.5 44.8 33.9 48.0 48.7 47.7 48.6 500 24.9 44.6 36.7 43.2 63.3 59.9 45.4 65.9 67.1 64.2 64.7 1000 37.6 56.8 44.5 57.3 82.1 81.3 58.5 83.4 85.5 82.6 83.2

As shown in the results of Table 4 above, the effect of inhibiting the MMP-1 production was higher in the case of mixing ginsenoside Rd and Rb2 than when using ginsenoside Rd and Rb2 alone. When the extract was mixed with 2: 1 mixture of ginsenoside Rd and Rb2, the effect of TGF-β was better than that of control TGF-β.

Test Example  4: Cytotoxicity test

Fibroblasts were inoculated into IMDM medium supplemented with 10% FBS at a cell concentration of 5 × 10 ⁵ cells and cultured in a 5% CO ₂ incubator at 37 ° C. for 24 hours. After the culture, the culture medium was removed, and the mixture of the ginsenosides Rd and Rb2 prepared in Preparation Examples 1 and 2, and the mixture obtained by killing this mixture and the mixture of the extracts was added to a final concentration of 50, 100, 500 and 1000 μg / After incubation for 24 hours, 100 μl of MTT (3- [4,5-dimethylthiazol-2yl] -2,5-diphenyltetrazolium boromide, Sigma, USA) was added to each well (3 mg / ml) for 4 hours. After removing the supernatant, 150 μl of dimethylsulfoxide was added, and the resulting formazan was shaken by shaking for 30 minutes. Absorption was measured at 540 nm using a multimicroplate reader (Molecular device Spectra max 190). Cell viability was calculated according to the following equation and the results are shown in Table 5 below.

Cell survival rate (%) = absorbance of sample-added group / absorbance of control group 100

sample
density
(占 퐂 / ml) Cell survival rate (%) Red ginseng
extract Gin Senocide Rd Ginsenoside Rb2 Gin Senocide Rd + Rb2 Gins (Rd + Rb2 = 2: 1): Kill the extract 1: 1 2: 1 3: 1 5: 1 1: 1 1: 5 1:10 0 100 100 100 100 100 100 100 100 100 100 50 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 500 99.8 100 100 100 100 100 100 100 100 100 1000 98.6 100 99.4 99.7 100 100 100 100 100 100

As shown in the results of Table 5, it was confirmed that the samples which were mixed with the ginsenosides Rd, Rb2, and ginsenosides Rd and Rb2, and the extracts were not cytotoxic at the concentration of 1000 μg / ml.

Example  1: Cream production

Creams were prepared according to the composition of Table 6 below for the clinical test of skin wrinkle-reducing effect and skin safety of cosmetic compositions containing the mixture of ginsenosides Rd and Rb2 of the present invention and kiss extract.

Comparative Example  1 ~ 3: Cream production

A cream containing only a 2: 1 mixture of ginsenoside Rd and Rb2, containing only red ginseng extract or replaced with purified water was prepared as a comparative example as shown in the following Table 6.

ingredient Content (% by weight) Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Ginsenoside (Rd + Rb2) 2.0 2.0 - - Kill Extract 10.0 - - - Red ginseng extract - - 4.5 - Pro-type glycerin monostearate 2.0 2.0 2.0 2.0 Stearyl alcohol 2.2 2.2 2.2 2.2 Stearic acid 1.5 1.5 1.5 1.5 Wax 1.0 1.0 1.0 1.0 Polysorbate 60 1.5 1.5 1.5 1.5 Sorbitan stearate 0.6 0.6 0.6 0.6 Purified vegetable oil 1.0 1.0 1.0 1.0 Squalane 3.0 3.0 3.0 3.0 Mineral oil 5.0 5.0 5.0 5.0 Trioctanoin 5.0 5.0 5.0 5.0 Dimethicone 1.0 1.0 1.0 1.0 Sodium magnesium silicate 0.1 0.1 0.1 0.1 glycerin 5.0 5.0 5.0 5.0 Betaine 3.0 3.0 3.0 3.0 Triethanolamine 1.0 1.0 1.0 1.0 Sodium hyaruronate 4.0 4.0 4.0 4.0 Preservative, fragrance, pigment a very small amount a very small amount a very small amount a very small amount Purified water Balance Balance Balance Balance Sum 100 100 100 100

Test Example  5: Skin wrinkle improvement effect

The skin wrinkle improving effect of the cosmetic composition containing the ginsenosides Rd and Rb2 and the extract of the present invention was measured in 60 healthy women aged 35 to 50 years. First, Example 1 was applied to Group A, Comparative Example 1 was applied to Group B, Comparative Example 2 was applied to Group C, and Formulations of Comparative Example 3 were applied to the face portion twice a day (morning and evening) 3 months. After 3 months, the improvement of wrinkles was evaluated by image analysis of questionnaires and wrinkles.

The subjects' questionnaires were judged to be four stages of no improvement, slight improvement, moderate improvement, and considerable improvement as compared with before use. The results are shown in Table 7 below.

The evaluation of the wrinkles by the image analysis was performed by taking replica under the eyes before the start of the experiment and collecting the replica immediately after the end of the experiment from the same area under the eyes and by analyzing the image by two dimensional analysis of the wrinkles, Were measured. The results of the measurement of the wrinkle density by image analysis are shown in Table 8 below as a reduction rate relative to the wrinkle density before use.

sample No improvement Slight improvement Moderate improvement A significant improvement Example 1 2 3 5 5 Comparative Example 1 4 5 4 2 Comparative Example 2 10 4 One - Comparative Example 3 11 4 - -

sample Wrinkle density reduction rate (%) Example 1 49 Comparative Example 1 36 Comparative Example 2 18 Comparative Example 3 11

From Tables 7 and 8, it can be seen that the effect of improving the skin wrinkles of Example 1 containing a mixture of ginsenoside Rd and Rb2 and killing extract was excellent.

Test Example  6: Skin moisturizing effect

The skin moisturizing effect of the cosmetic composition containing the ginsenosides Rd and Rb2 and the extract of Killenia rata according to the present invention was measured in 60 healthy women aged 35 to 50. Firstly divided into four groups and group A Example 1 was applied to Group B, Comparative Example 1 was applied to Group B, Comparative Example 2 was applied to Group C, and Formulations of Comparative Example 3 were applied to the face group for 4 weeks twice a day (morning and evening). Before the start of the experiment, the two sites to be measured were determined, and the moisture content of the skin was measured three times and then applied (2 times / day) for 4 weeks. Then, using the skin moisture content measuring device, Was measured. The measurement results are summarized in Table 9.

sample  The initial (D0) average 4 weeks (D28) Average Moisture Increase (%) Example 1 35 45 28.6 Comparative Example 1 36 44 22.2 Comparative Example 2 34 39 14.7 Comparative Example 3 36 39 8.3

From Table 9, it can be seen that the skin moisturizing effect of Example 1 containing ginsenoside Rd and Rb2 mixed extract and killed extract was excellent.

Test Example  7: Stability test of formulation

For the stability test of the formulation of the cosmetic composition of the present invention, the above Examples and Comparative Examples were stored in an opaque glass container in a thermostat kept constant at 45 캜 for 12 weeks, and kept at 4 캜 for a completely shielded After being stored for 12 weeks in an opaque glazed container in a refrigerator, the degree of discoloration, separation, and separation were measured, and the results are shown in Table 10 below. At this time, the degree of discoloration, separation and separation of the product was classified into the following six grades and evaluated.

0: No change 1: Very little separation (discoloration, change)

2: little separation (discoloration, discoloration) 3: slight separation (discoloration, discoloration)

4: Severely separated (discolored, detached) 5: Severely separated (discolored, detached)

division Stability of formulation Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 45 ° C 4 ℃ 45 ° C 4 ℃ 45 ° C 4 ℃ 45 ° C 4 ℃ discoloration 0 0 0 0 0 0 0 0 Fling 0 0 0 0 0 0 0 0 detach 0 0 0 0 0 0 0 0

As can be seen from Table 10, the examples and the comparative examples were stable at 45 ° C and 4 ° C with almost no discoloration, separation, and separation, and the ginsenosides Rd and Rb2 and the killed extract were stable in the formulation .

The cosmetic composition containing the ginsenosides Rd and Rb2 of the present invention and the killed extract can be prepared in the following various formulations.

Example  2: Flexible lotion

Flexible lotions containing ginsenosides Rd and Rb2 and killing extracts in the compositions listed in Table 11 below were prepared in a conventional manner.

ingredient Content (% by weight) Ginsenoside Rd + Rb2 (2: 1) 1.5 Kill Extract 7.5 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl vinyl polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Example  3: Nourishing lotion ( Milk lotion )

Nutritional lotions containing ginsenosides Rd and Rb2 and killing extracts in the compositions listed in Table 12 below were prepared in a conventional manner.

ingredient Content (% by weight) Ginsenoside Rd + Rb2 (2: 1) 3.0 Kill Extract 15.0 glycerin 5.1 Propylene glycol 4.2 Tocopheryl acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia nut oil 2.5 Polysorbate 20 1.6 Sorbitan sesquiterate 1.6 Propylparaben 0.6 Carboxyl vinyl polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Example  4: emulsion  Base

Emulsion bases containing mixtures of ginsenosides Rd and Rb2 and killing extracts in the compositions listed in Table 13 below were prepared in a conventional manner.

ingredient Content (% by weight) glycerin 3.00 Disodium iodide 0.02 Polyglyceryl-3-methylglucoside distearate 1.50 Cetearyl alcohol 0.50 Caprylic / capric triglyceride 7.00 Polyacrylamide & C13-14 Isoparaffin & Laureth-7 0.60 Ginsenoside Rd + Rb2 (2: 1) 3.0 Kill Extract 15.0 Incense, preservative a very small amount Purified water Balance system 100

Example  5: Massage  cream

Massage creams containing mixtures of ginsenosides Rd and Rb2 and killing extracts in the compositions listed in Table 14 below were prepared in a conventional manner.

ingredient Content (% by weight) Ginsenoside Rd + Rb2 (2: 1) 3.0 Kill Extract 15.0 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Wax 2.1 Tocopheryl acetate 0.1 Polysorbate 60 2.4 Carboxyl vinyl polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Example  6: Pack

A pack containing ginsenosides Rd and Rb2 and killing extracts in the compositions described in Table 15 below was prepared in a conventional manner.

ingredient Content (% by weight) Ginsenoside Rd + Rb2 (2: 1) 4.5 Kill Extract 22.5 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 glycerin 4.5 Carbopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water Balance system 100

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the technical scope of the present invention is not limited to the disclosed exemplary embodiments. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (6)

A cosmetic composition for improving and moisturizing wrinkles containing a mixture of ginsenosides Rd and Rb2 and killed extract as an active ingredient. The cosmetic composition according to claim 1, wherein the mixture of ginsenosides Rd and Rb2 is a mixture of ginsenosides Rd and Rb2 in a weight ratio of 2: 1, respectively. The cosmetic composition for improving and improving wrinkles according to claim 1, wherein the mixture of ginsenosides Rd and Rb2 and the killed extract are contained in a weight ratio of 1: 1 to 10. [Claim 3] The cosmetic composition according to claim 1, wherein the ginsenosides Rd, Rb2 and kiss extract are nanocapsulated, microencapsulated or nano-sized. The cosmetic composition according to any one of claims 1 to 4, wherein the mixture of ginsenosides Rd and Rb2 and the killed extract are contained in an amount of 0.1 to 15% by weight based on the total weight of the cosmetic composition, Cosmetic composition. The cosmetic composition according to any one of claims 1 to 4, wherein the cosmetic composition is at least one selected from the group consisting of a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, Characterized in that it consists of any one of the formulations selected from the group consisting of creams, essences, packs, soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders and eye shadows (EN) Cosmetic composition for improvement and moisturizing.