JPH11158054A - Skin lotion - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin lotion, exhibiting an excellent collagen production- stimulating effect and having excellent wrinkle-improving effect, skin roughness- preventing and improving effects, moisture-retaining effect and somberness- improving effect by including a yeast extract and the extract of a plant belonging to the genus Fagus. SOLUTION: This skin lotion contains (A) a yeast extract (yeast extract, yeast degradation product or yeast degradation product extract) obtained from a beer yeast, a wine yeast or the like, belonging to the genus Saccharomyces, and (B) the extract of a plant belonging to the genus Fagus, preferably the extract of the plumule sites of Fagus sylvaitca L. The components are preferably contained in amounts of 0.0001-5 wt.%, respectively, in the skin preparation for external use. The yeast degradation product of the component A may be a degradation product obtained by the self digestion, protease degradation or acidic hydrolysis of the yeast.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a skin comprising a yeast extract, a yeast digest, a yeast digest (hereinafter collectively referred to as yeast extract), and an extract of a Beech plant. More specifically, the external preparation has an effect of promoting the production of collagen in dermal fibroblasts, is effective in preventing or improving skin aging symptoms such as generation of wrinkles and rough skin, and has an effect of moisturizing and improving dullness. It is intended to provide a skin external preparation which can also exert the following.

[0002]

2. Description of the Related Art One of the aging phenomena of the skin is generation of wrinkles and rough skin. It is considered that such a phenomenon is caused by the progress of collagen cross-linking due to aging and the degradation of collagen by ultraviolet rays or the like. Accordingly, many cosmetics containing collagen and cosmetics containing a collagen production promoting substance have been developed.

[0003] Collagen in the dermis is produced by dermal fibroblasts and degraded by collagenase produced by dermal fibroblasts. Therefore, cosmetics have been proposed in which a collagenase inhibitor that inhibits the activity of collagenase is blended to suppress the degradation of collagen and to suppress wrinkles and rough skin.

[0004] However, conventional cosmetics containing collagen have problems in percutaneous absorption and stability, and have not fundamentally improved the amount of collagen in the dermis. In addition, a sufficient anti-aging effect has not been obtained in cosmetics containing a collagen production promoter or a collagenase inhibitor, and more effective anti-aging agents have been desired.

[0005]

DISCLOSURE OF THE INVENTION The present invention relates to an external preparation for skin which has a sufficient collagen production promoting action on dermal fibroblasts, suppresses wrinkles and rough skin, and is excellent in safety and stability. The purpose is to provide.

[0006]

Means for Solving the Problems The present inventors have conducted intensive studies in order to solve the above-mentioned problems, and as a result, a yeast extract,
When combined with a beech genus extract in an external preparation for skin, it not only exhibits an excellent collagen production promoting effect, but also has an effect of preventing skin roughness, improving skin roughness, moisturizing, and improving dullness. Have been found, and the present invention has been completed.

The yeast for obtaining the yeast extract to be incorporated in the present invention has been used for a long time in the food field such as alcohols, bread, miso, soy sauce, and pickles. In addition, it has been proposed to incorporate these yeast extracts into cosmetics, and a whitening agent using a yeast extract in combination with an enzyme hydrolyzate of placental tissue (Japanese Patent Application Laid-Open No.
No. 3-142515), a whitening effect of a yeast extraction solution containing ethanol and RNA (JP-A-61-26000).
No. 9), a heteropolysaccharide derived from a yeast fermented product (Japanese Unexamined Patent Publication No.
JP-A-128694), and the antioxidant action of a yeast culture product (JP-A-4-20590).

[0008] In addition, as an application of a beech plant to an external preparation for skin, an epithelial preparation containing an ethanol extract as an active ingredient (Japanese Patent Publication No. 4-33765) and a slimming agent containing an European beech extract as an active ingredient (French Patent 2662
No. 28), a composition for the oral cavity containing a beech family Virginia vulgaris extract as an active ingredient (JP-A-6-298633),
Cosmetic containing beech seed coat and gall extract as antioxidant (JP-A-7-126618), and extract from beech tree germ to promote keratinocyte protein synthesis (JP-A-9-126) No. 227397) has already been disclosed.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, yeast for obtaining a yeast extract is a yeast belonging to the genus Saccharomyces , such as brewer's yeast, sake yeast, wine yeast, and the like.
Baker's yeast and the like are used.

In the case of using a decomposition product of yeast by self-digestion, a 10- to 100-fold amount of purified water is added to the washed wet cells at 35-45 ° C. for about 24-72 hours. After digestion, if necessary, a yeast digest obtained by filtering, freeze-drying or concentrating under reduced pressure can be used.

In the case of using a degradation product of yeast with a protease, 10 to 100 times the amount of purified water is added to the washed wet cells, and the protease is added in an amount of 20 to 1 kg per 1 kg of wet cells. About 500,000 units are added, and the reaction is carried out at about the enzyme activity temperature for 12 to 48 hours. Next, after inactivating the enzyme, a yeast degradation product obtained by adding an appropriate amount of an aqueous solvent, if necessary, performing an operation such as centrifugation, filtering, freeze-drying or concentrating under reduced pressure can be used.

When an acid hydrolyzate of yeast is used, an acid such as hydrochloric acid is added to the wet cells after washing, and the acid is added to the cells.
Hydrolyze at 60 ° C. for 3-8 hours with occasional stirring. Thereafter, the product is neutralized with an alkali such as sodium hydroxide, filtered if necessary, and then lyophilized or concentrated under reduced pressure to obtain a yeast degradation product.

These yeast digests may be used as a yeast extract as they are, or a yeast digest extract obtained by extracting the yeast digests by the following method may be used as the yeast extract.

In order to obtain an extract from yeast, the cells after culturing are collected, washed with purified water or the like to obtain wet cells, and the wet cells are destroyed as they are or by high frequency. Used.

[0015] Water,
Ethanol, methanol, isopropanol, isobutanol, n-hexanol, methyl amyl alcohol, 2-
Monohydric alcohols such as ethylbutanol and n-octyl alcohol, glycerin, ethylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, propylene glycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, triethylene glycol, 1, 3-
One or two or more aqueous solvents such as polyhydric alcohols such as butylene glycol and hexylene glycol or derivatives thereof are selected and added as an extraction solvent for extraction. The extraction solvent is not particularly limited as long as it is an aqueous solvent as described above. However, from the viewpoint of safety and stability when blended in an external preparation for skin, purified water, ethanol, 1,3-butylene glycol, glycerin, propylene It is preferable to use glycol alone or in combination of two or more.

Examples of the extraction method include a method of immersion extraction at room temperature, in a cooled or heated state, and a method of extraction using a distillation method such as steam distillation. These methods may be used alone or in combination of two or more. Extraction is performed by combining the above.

The ratio of the wet cells to the solvent at the time of extraction is not particularly limited.
~ 1000 times by weight, especially 0.5 ~ in terms of extraction operation and efficiency
100 weight times is preferred. The extraction temperature is conveniently in the range from room temperature to the boiling point of the solvent under normal pressure, and the extraction time varies depending on the extraction temperature and the like, but is preferably in the range of 2 hours to 2 weeks.

The extract obtained from the yeast cells and the yeast hydrolyzate using an aqueous solvent can be used as it is, but it can be deodorized, decolorized and concentrated as long as the effects of the present invention are not lost. Or the like, or may be used as a fraction by using column chromatography or the like. These extracts, deodorized products, purified products, and fractionated products can be dried by removing the solvent from them, and can be further dissolved in a solvent such as alcohol.
Alternatively, it can be incorporated into an external preparation for skin in the form of an emulsion.

[0019] The plant of the Fagus used in the present invention (Fagus) is a kind of beech family (Fagaceae) plant. Beech plants (Shirobuna, buckwheat, Fagus cren)
ata Bl.), American beech ( Fagus grandifolia Ehrh .; F
agus ferruginea Ait .; Fagus americana Sweet.), Inubuna (Kurobuna, Fagus japonica Maxim .; Fagus long
ipetiolata Seem .; Fagus sylvatica var. longipes Oli
v .; Fagus sinensis Oliv.), European beech ( Fagus s
ylvatica L.). These beech ( Fagu
s ) Among plants, it is preferable to use European beech ( Fagus sylvatica L.) from the viewpoint of the effect of the present invention.

The extraction site for obtaining the extract of the Beech genus plant is not particularly limited, and the bark and its mucus, fruits, seeds,
Examples include flowers, branches, leaves, and the like, and among them, extracts of germ sites are most preferable from the viewpoint of the effects of the present invention. The extraction method is the same as the method for obtaining an extract of the yeast and the yeast degradation product.

Further, in the present invention, it is most preferable to extract the germ of a Beech plant with purified water. More specifically, after treating the collected beech germ with high frequency, the cell membrane is crushed, the contents of the cells are recovered by low-temperature extraction using purified water as a solvent, and the insoluble residue is filtered. It is preferable to use an extract obtained by removing organic acids by removing the organic acid by concentration and permeation treatment under reduced pressure, and then removing bacteria by a membrane filter.

The amounts of these yeast extract and beech plant extract to be added to the external preparation for skin are considered to be 0.0001 to 5% by weight, respectively, in view of their effects, flavor and color tone when added. It is desirable to do. 0.00%
If it is less than 01% by weight, a sufficient effect cannot be obtained,
There is no need to mix too much.

In the present invention, the above-mentioned yeast extract and beech plant extract may be blended to provide an external preparation for skin. The external preparation for skin may be in the form of a lotion, emulsion, cream, ointment or the like. Can be. Further, lotions such as flexible lotion, astringent lotion, lotion for washing, etc., creams such as emollient cream, moisture cream, massage cream, cleansing cream, makeup cream, emollient emulsion, moisture emulsion, nourishing It can also be provided as cosmetics in various preparation forms, such as emulsions such as emulsions and cleansing emulsions, packs such as jelly packs, peel-off packs, wash-off packs, powder packs, serums, and face wash.

In the present invention, other anti-aging components, whitening components, moisturizing components, anti-inflammatory agents, ultraviolet absorbers, etc.
Other active ingredients can also be used in combination, and can be provided as cosmetics such as whitening cosmetics, sunscreen cosmetics, skin protection cosmetics and the like, or quasi-drugs.

[0025]

EXAMPLES First, production examples of yeast extract and beech plant extract used in Examples of the present invention will be described.

[Production Example 1] Yeast extract 1 Beer yeast is suspended in a 30% by weight ethanol solution, and this suspension is sufficiently homogenized with a homomixer, followed by centrifugation and filtration.

[Production Example 2] Yeast extract 2 Sake yeast is suspended in purified water and autolyzed at 45 ° C for 48 hours. Next, this was freeze-dried, and a 30% by weight of 1,3-butylene glycol solution was added thereto and sufficiently stirred and extracted.
Centrifuge and filter.

[Production Example 3] Yeast extract 3 Wine yeast is suspended in purified water, a protease (Actinase A, manufactured by Kaken Pharmaceutical Co., Ltd.) is added, and the mixture is reacted at 37 ° C for 24 hours. Next, after inactivating the enzyme, propylene glycol is added to a concentration of 30% by weight, followed by centrifugation and filtration.

Production Example 4 Yeast Extract 4 Beer yeast is suspended in 3% hydrochloric acid and hydrolyzed at a temperature of 50 ° C. for 6 hours with occasional stirring. After disassembly,
Neutralize with sodium hydroxide, filter with a membrane filter, concentrate under reduced pressure and desalinate.

[Preparation Example 5] Beech extract 1 Fresh leaves of beech are pulverized, immersed in a 10 times volume of 50% by volume aqueous 1,3-butylene glycol solution for 10 days at room temperature, and filtered.

[Production Example 6] Beech extract 2 After treatment of beech germ with high frequency, the cell membrane is destroyed, and the contents of the cells are recovered by low-temperature extraction using purified water as a solvent and filtered.

The effect of promoting the collagen production when the yeast extract and the beech extract were used in combination was examined using cultured human dermal fibroblasts. Human dermal fibroblasts were cultured in Dulbecco's minimum essential medium supplemented with fetal calf serum, and after 24 hours, the medium was replaced with the above-mentioned medium supplemented with each of the production examples shown in Table 1, and the culture was continued for 7 days. Procollagen in the culture supernatant was converted to a commercially available kit (Takara Procollagen Type I C-
Enzyme-linked immunosorbent assay (ELIS) using Peptide EIA Kit
A). At that time, the culture was performed without adding a collagen production promoter as a control, and the procollagen production rate (%) was calculated, assuming that the amount of procollagen production in the control was 100%. Procollagen is a precursor of collagen, and procollagen is converted into collagen after being secreted from the cells.

[0033]

[Table 1]

The results of measuring the collagen production rate are shown in Table 1. As is clear from Table 1, a significant increase in procollagen production was observed by culturing fibroblasts by simultaneously adding yeast extract and beech extract, which are the components of the present invention. No cytotoxicity to fibroblasts was observed in the concentration range in which this experiment was performed.

Examples 1 to 8 and Comparative Examples 1 to 7
W-emulsion type serum A yeast extract and a beech extract are blended in the amounts shown in Table 2 and
/ W emulsified cosmetic liquid was prepared. (Prescription) (1) Squalane 5.0 (% by weight) (2) White petrolatum 2.0 (3) Beeswax 0.5 (4) Sorbitan sesquioleate 0.8 (5) Polyoxyethylene oleyl ether (20EO) 1.2 (6) Methyl parahydroxybenzoate 0.1 (7) Propylene glycol 5.0 (8) Purified water Amount based on the total amount of 100 (9) 1.0% by weight aqueous solution of carboxyvinyl polymer 20.0 (10) Hydroxidation Potassium 0.1 (11) Ethanol 5.0 (12) Yeast extract and beech extract Amount shown in Table 2 (13) Fragrance 0.2 Production method: Mix oil phase components (1) to (5) and heat to 75 ° C Dissolve and homogenize. On the other hand, the aqueous phase components of (6) to (8) are mixed,
Dissolve and heat to 75 ° C. and pre-emulsify by gradually adding the oil phase component. After adding (9), the mixture is emulsified uniformly with a homomixer, and (10) is added to adjust the pH. After cooling 4
At 0 ° C, add (11) to (13), mix and homogenize.

[0036]

[Table 2]

Using the above Examples 1 to 8 and Comparative Examples 1 to 7, use tests were performed. In the use test, wrinkle improvement effect, skin roughness improvement effect, dullness improvement effect, and moisturizing effect were examined. First, a method for evaluating each effect will be described.

[Wrinkle Improvement Effect] The wrinkle improvement effect was evaluated by an actual use test for 6 months. As panelists, women in their 40s to 60s who had skin symptoms such as remarkable wrinkles were used, and 20 people were included in each group. The use test was performed by using each of the examples and the comparative examples blindly for each group. The skin condition was observed before the use test and after the use test, and the wrinkle improvement was evaluated in three stages of “improvement”, “slight improvement”, and “no change”, and judged according to the following criteria.

(Judgment) ：: The proportion of the subject showing improvement and slight improvement is 80% or more ○: The proportion of the subject showing improvement and slight improvement is 50% or more and less than 80% △: The subject is improved and slightly improved : 30% or more and less than 50% ×: The rate of improvement of the subject and slightly improvement of less than 30%

[Effect of Improving Skin Roughness] A group of 20 male panels was coated with a 10% by weight aqueous solution of sodium lauryl sulfate as a surfactant for several days on several parts of the forearm.
Induced rough skin. Thereafter, the Example and the Comparative Example were applied twice a day to the rough skin area, and 5 days after the start of the application, the state of the skin surface was observed by a replica method. Then, the improvement of the rough skin was evaluated by the following score. In addition, the score before the application of Examples and Comparative Examples of the site that induced rough skin,
All were 2 or less.

(Skin condition score) Score 1: Elimination of skin sulcus and crusts and exfoliation of a wide range of stratum corneum are observed. : Skin ridges are recognized but flat and ridges are indistinct. 4: Skin ridges and ridges are clear. 5: Skin ridges and ridges are clear and regular. (Judgment) ◎: Score 3 80% or more of the subjects evaluated above ○: 50% or more of the subjects evaluated to score 3 or more 80
%: 30% or more of the subjects evaluated as score 3 or more.
Less than 30%: Less than 30% of subjects evaluated as score 3 or more

[Effect of Improving Dullness] Twenty subjects suffering from dullness of the skin were taken as a group, and Examples 1 to 8 and Comparative Example 1 were used.
Comparative Example 7 was applied to the face twice a day for 3 months, and the effect of improving dullness was evaluated according to the following criteria. The results are shown in Table 3.

(Evaluation criteria) Significant effect: almost no dullness was felt Effective: fairly no dullness was felt somewhat effective: little dullness was no longer felt invalid: no change was felt (judgment) ：: The percentage of subjects who show significant, effective, and slightly effective is 8
0% or more ：: The proportion of subjects who show significant, effective, and somewhat effective is 5
0% or more and less than 80% △: The proportion of subjects showing significant, effective, and somewhat effective is 3
0% or more and less than 50% ×: The ratio of subjects showing significant, effective, and slightly effective is 3
Less than 0%

[Moisturizing Effect] The moisturizing effect was measured by applying 0.01 ml / cm ^{2 of the} example to the inner part of the forearm in a thermo-hygrostat at a temperature of 20 ° C. and a relative humidity of 50%. High frequency impedance meter (Skico, manufactured by IBS)
n200). As a control, the water content of the epidermis was measured in a portion where the Examples and Comparative Examples were not applied, and the difference was determined based on the following criteria.

(Judgment) ：: The amount of water increased by 40 μS or more ：: The amount of water increased from 25 μS to less than 40 μS Δ: The amount of water increased from 10 μS to less than 25 μS ×: The amount of water did not increase or increased less than 10 μS

[0046]

[Table 3]

As shown in Table 3, by using the yeast extract and the beech extract in combination, an excellent serum having an effect of improving wrinkles, an effect of improving skin roughness, an effect of improving dullness and a moisturizing effect was obtained. In particular, in Example 2, Example 4, Example 6, and Example 8 containing a beech germ extract, excellent evaluations were obtained for all effects. On the contrary,
In Comparative Examples 1 to 4 containing only yeast extract, the effect of improving dullness and moisturizing effect was 50%.
Although evaluated by the above panelists as effective, wrinkle improving effects and skin roughness improving effects were not so much recognized. In Comparative Examples 5 and 6 containing only the beech extract, the effect of improving skin roughness was evaluated to be effective with 50% or more of panelists, but the effect of improving dullness was hardly recognized.

In the above use tests, none of the groups using any of the examples reported any skin irritation such as pain and itchiness or skin symptoms such as allergic reaction. In addition, deterioration of the emulsified state, sedimentation of the components,
No change in the state of the preparation, such as deterioration, was observed.

Next, the formulation of another embodiment of the present invention will be described. [Example 9] Liquid skin external preparation (1) Glycerin 5.0 (% by weight) (2) Propylene glycol 4.0 (3) Ethanol 10.0 (4) Yeast extract 10.5 (5) Beech extract 10 2 (6) Methyl paraoxybenzoate 0.1 (7) Purified water 80.2 Production method: Dissolve (6) in (3), add to (7), and add (1), (2),
(4) and (5) are sequentially added, mixed and homogenized.

Example 10 Lotion (1) 1,3-butylene glycol 3.0 (% by weight) (2) Sorbitol 2.0 (3) Ethanol 10.0 (4) 1% by weight aqueous solution of carboxyvinyl polymer 10.0 (5) Yeast extract 2 0.5 (6) Beech extract 2 0.2 (7) Methyl parahydroxybenzoate 0.1 (8) Fragrance 0.1 (9) Purified water 74.1 Production method: (7) , (8) is dissolved in (3) and added to (9), (1),
After (2), (5), and (6) are added and mixed sequentially, (4) is added, and mixed and homogenized.

Example 11 O / W Emulsion Ointment (1) White Vaseline 25.0 (wt%) (2) Stearyl Alcohol 15.0 (3) Sodium Lauryl Sulfate 1.0 (4) Paraoxybenzoic Acid Butyl 0.1 (5) Yeast extract 3 0.5 (6) Beech extract 2 0.15 (7) Purified water 58.25 Production method: Mix the oil phase components (1) to (4) and heat to 75 ° C. Dissolve and homogenize. The oil phase component is added to (7) heated to 75 ° C. to emulsify. After cooling, (5) and (6) are sequentially added, mixed and homogenized at 40 ° C.

[Example 12] W / O emulsified cream (1) Beeswax 3.0 (wt%) (2) Adsorbed and purified lanolin 10.0 (3) Squalane 30.0 (4) Solid paraffin 2.0 ( 5) Microcrystalline wax 5.0 (6) Hexyldecyl adipate 10.0 (7) Sorbitan sesquioleate 3.5 (8) Polyoxyethylene (50EO) hydrogenated castor oil 1.0 (9) 1,3- Butylene glycol 5.0 (10) Purified water 29.5 (11) Methyl parahydroxybenzoate 0.2 (12) Yeast extract 4 0.5 (13) Beech extract 10.3 Manufacturing method: (1) to (8) The oil phase components are mixed and heated to 75 ° C. to dissolve and homogenize. On the other hand, the aqueous phase components (9) to (11) are mixed,
Dissolve and heat to 75 ° C., add to the above oil phase components and emulsify uniformly with a homomixer. After cooling, at 40 ° C (1
2) and (13) are sequentially added and mixed.

Example 13 Makeup Base Cream (1) Stearic acid 12.0 (% by weight) (2) Cetanol 2.0 (3) Glyceryl tri-2-ethylhexanoate 2.5 (4) Self-emulsification Type glyceryl monostearate 2.0 (5) Propylene glycol 10.0 (6) Potassium hydroxide 0.3 (7) Purified water 68.6 (8) Titanium oxide 1.0 (9) Bengala 0.1 ( 10) Yellow iron oxide 0.4 (11) Fragrance 0.1 (12) Yeast extract 2 0.5 (13) Beech extract 1 0.5 Manufacturing method: The oil phase components of (1) to (4) are mixed and mixed. Heat to ° C to make uniform. On the other hand, the aqueous phase components (5) to (7)
The mixture is heated and dissolved at 5 ° C. to make the mixture uniform, and the pigments (8) to (10) are added thereto and uniformly dispersed by a homomixer. The oil phase component is added to the aqueous phase component, emulsified by a homomixer, cooled, and (11) to (13) are added and mixed at 40 ° C.

Example 14 Emulsion Foundation (1) Stearic acid 2.0 (% by weight) (2) Squalane 5.0 (3) Octyldodecyl myristate 5.0 (4) Cetanol 1.0 (5) Decaglyceryl monoisopalmitate 9.0 (6) 1,3-butylene glycol 6.0 (7) Potassium hydroxide 0.1 (8) Methyl parahydroxybenzoate 0.1 (9) Purified water 53.2 ( 10) Titanium oxide 9.0 (11) Talc 7.4 (12) Bengala 0.5 (13) Yellow iron oxide 1.1 (14) Black iron oxide 0.1 (15) Fragrance 0.1 (16) Yeast Extract 1 0.3 (17) Beech extract 2 0.1 Production method: The oil phase components of (1) to (5) are mixed and heated to 75 ° C. to make uniform. On the other hand, the aqueous phase components (6) to (9)
The mixture is heated to 5 ° C. and dissolved to make the mixture uniform, and the pigments (10) to (14) are added thereto and uniformly dispersed by a homomixer. The oil phase component is added to the aqueous phase component, uniformly emulsified by a homomixer, cooled, and (15) to (17) are sequentially added and mixed at 40 ° C.

Example 15 Hand cream (1) Cetanol 4.0 (% by weight) (2) Vaseline 2.0 (3) Liquid paraffin 10.0 (4) Glyceryl monostearate 1.5 (5) Polyoxyethylene (60EO) glyceryl isostearate 2.5 (6) Tocopherol acetate 0.2 (7) Glycerin 20.0 (8) Methyl parahydroxybenzoate 0.1 (9) Purified water 59.2 (10) Yeast Extract 4 0.4 (11) Beech extract 2 0.1 Production method: Mix and dissolve the oil phase components (1) to (6) and heat to 75 ° C. On the other hand, the aqueous phase components (7) to (9) are mixed and dissolved and heated to 75 ° C. Then, the oil phase component is added to the water phase component and pre-emulsified, then uniformly emulsified and cooled with a homomixer, and (10) and (11) are sequentially added and mixed at 40 ° C.

[0056]

As described in detail above, by incorporating the yeast extract and the beech genus plant extract in the present invention in combination, an excellent collagen production promoting effect is exhibited, wrinkle improving effect, skin roughness prevention and An external preparation for skin excellent in skin roughness improvement effect, moisturizing effect, and dullness improvement effect was obtained.

Claims (9)

[Claims] 1. An external preparation for skin comprising an extract obtained from an yeast using an aqueous solvent and an extract of a Beech plant. 2. An external preparation for skin, comprising a yeast decomposition product and a beech plant extract. 3. An external preparation for skin, comprising: an extract obtained by using an aqueous solvent from a yeast decomposition product; and an extract of a Beech plant. 4. The yeast digest is a yeast digest obtained by autolyzing yeast.
The external preparation for skin according to claim 2 or 3. 5. The external preparation for skin according to claim 2, wherein the yeast digest is a yeast digest obtained by digesting yeast with a protease. 6. The external preparation for skin according to claim 2, wherein the yeast digest is a digest obtained by hydrolyzing yeast with an acid. 7. The external preparation for skin according to claim 1, wherein the yeast is a yeast belonging to the genus Saccharomyces. 8. The external preparation for skin according to claim 1, wherein the extract of a Beech plant is an extract from a germ of a Beech plant. 9. The external preparation for skin according to claim 1, wherein the extract of a beech plant is a purified water extract of the germ of European beech ( Fagus sylvatica L.). .