JPH09227397A - Preparation for external use for skin - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a preparation for external use for skin, consisting essentially of an extract from a plumule of a tree of Fagus crenata Blume, Fagus sylvatica L., etc., and effective in preventing the skin from aging due to the ability to activate the protein synthesis in a keratinocyte, thereby rejuvenate the skin and improve the humectant properties. SOLUTION: This preparation for external use for skin consists essentially of an extract from a plumule of a tree of Fagus crenata Blume, Fagus sylvatica L., etc., preferably in an amount of 0.2-20wt.%, practically 0.4-10wt.% therein. A cream, an ointment, a milky lotion, a gel or a liquid form is preferred as the dosage form. A physiologically active substance such as a phospholipid, vitamins, amino acids, arbutin, kojic acid, a placental extract, a blood serum albumin, hyaluronic acid or collagen as a secondary ingredient can be blended in the objective preparation for external use for the skin.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an external preparation for skin,
In particular, it relates to a skin external preparation containing an extract from a tree bud as a main component.

[0002]

2. Description of the Related Art With the expectation of physiological activity on the skin, attempts have been made for a long time to mix an extract from a natural material into an external preparation for skin. Natural materials used for such purposes are mainly animal organs (brain, liver, spleen,
Blood) and plant tissues (leaf, stem, root, flower, seed, etc.).

The physiologically active components contained in the extracts from these natural materials include various phospholipids, vitamins, amino acids, proteins, arbutin, kojic acid, placenta extract, serum albumin (eg bovine serum albumin). ),
Glycosaminoglycans, hyaluronic acid, collagen, elastin, glycosides, polyphenols (tannin), flavonoids, etc. are known.

[0004]

However, these extracts elute certain components by treating the dry-preserved material with warm or hot water or various organic solvents.
Most of them were obtained by filtration and, if necessary, fractionation treatment, etc., and the physiologically active substance content was low except for some. In many cases, the essence of the active ingredient is not specified, and the fact that the effect is proven is limited.

[0005] In recent years, there has been a growing trend to convert animal-derived extracts to plant-derived extracts from the viewpoint of animal welfare and the like. However, it is not easy to find an extract from an animal organ or the like which has a high physiological activity and is therefore of plant origin and whose effect is comparable or superior to that of animal origin. Even if there is a plant resource containing a component exhibiting a useful physiological activity, the useful component is often altered by the above-mentioned general extraction method.

[0006] By the way, the tree has various metabolic precursors in winter.
(metabolic precursor) and powerful enzymes are accumulated, which prepares for the escape from the dormant state in winter, that is, germination.Therefore, there are various physiologically active substances useful for humans in the young shoots. , A medical science called "gemmotherapy" that started in Europe in the 1960s, based on the use of bioactive components hidden in the buds. It is also called. It is the Japanese beech among the trees
Fagus crenata is a deciduous tree that grows in the highlands of 600-1500 m, and plantations are rare, but Fagus silvati of the same genus
Ca is called European beech, and there are many afforestation sites in Central Europe. This European beech tree is called "sacrifice tree" in olden times, and is also called "eternal young tree" because of its balance of vitality and beauty of appearance. From the beech buds, a stabilized extract obtained by eluting a useful physiologically active component contained in the buds while suppressing the denaturation thereof as much as possible is, for example, Gattefosse (Gattefosse) company in France.
ttefosse SA) and is commercially available.

[0007]

The present inventor has noticed that the above extract contains various useful physiologically active components such as hormones, flavonoids and peptides, and the extract is used as a skin external preparation. In order to use it as an active ingredient, the inventors have made earnest studies.

That is, when the effect of the above extract on the skin was examined, it was found that the oxygen consumption of the cell itself was increased without affecting the cell proliferation and morphological changes of human fibroblasts and keratinocytes, In other words, it was found that the proliferation of skin cells was not toxic and that the protein synthesis of keratinocytes was increased, and the beginning of the present invention was obtained. Therefore, while preparing a cream containing the above extract, to create a skin replica targeting healthy volunteers, please have the above-mentioned cream applied daily to the site of the skin used to create the replica for a certain period of time, After that, we made another skin replica and compared the shapes of both replicas.As a result, it was found that the replica made in the second time showed less wrinkles in the skin than the replica made in the first time. Thus, the present invention has been completed.
In addition, if various nutrients and regulatory peptides (keratin, filaggrin, etc.) are generated by the activation of protein synthesis of keratinocytes and accumulate in the stratum corneum, this will retain water in the stratum corneum due to the osmotic effect. Which results in improved moisturization on the skin.

Therefore, the external preparation for skin according to the present invention is characterized in that it is mainly composed of the extract from the shoots of beech trees.

The dosage form of the external skin preparation according to the present invention may be a cream, ointment, emulsion, gel or liquid.

In the external preparation for skin according to the present invention, the content of the extract from beech tree shoots depends on the dosage form,
Considering the effect and cost, about 0.1-50.0% by weight is appropriate, preferably 0.2-20.0% by weight,
Practically, the range of 0.4-10.0% by weight is sufficient.

In the external preparation for skin according to the present invention, "creamy" refers to an oil-in-water type or a water-in-oil type emulsified and having a relatively high viscosity, and this agent is safe. It can contain an inorganic or organic pigment guaranteed. "Ointment" refers to an oil-like petroleum jelly-like substance containing almost no water, and this agent may contain an inorganic or organic pigment whose safety is guaranteed. "Emulsion" refers to an oil-in-water type or water-in-oil type that is emulsified and has relatively low viscosity and fluidity.This agent is an inorganic or organic compound whose safety is guaranteed. It may contain a pigment. The term “gel-like” refers to a state of being clear and having low viscosity having fluidity to high viscosity having no fluidity. “Liquid” refers to a liquid that is clear and has almost no viscosity and is close to water.
The skin external preparations in each of the above states, and specific examples of the cosmetic formulation include skin creams, lotions, lotions, lotions, liquid foundations, pressed powders, lipsticks, lip glosses and the like. Examples of the hair wash type include shampoo, rinse, treatment and the like.

The skin external preparation according to the present invention can naturally contain other physiologically active substance as an accessory ingredient. As a physiologically active substance that is this accessory ingredient,
It is a component contained in the extract that has been conventionally used, and various phospholipids, vitamins, amino acids, proteins, arbutin, kojic acid, placenta extract, serum albumin, and glycos mentioned in the beginning of this specification. Saminoglycan, hyaluronic acid, collagen, elastin, glycoside,
Examples thereof include polyphenols and flavonoids.

[0014]

EXAMPLES Next, the present invention will be described in more detail and specifically with reference to test examples and formulation examples. The appearance and the like of the extract from the young shoots of the beech tree used in the following test examples and formulation examples were as follows. Appearance: Brown to amber liquid pH: 5.1 Dry residue: 10.3 g / l Inorganic salt: 0.86 g / l Total nitrogen: 0.24 g / l Peptide: 1.5 g / l Also described in the formulation example. "Carbopol" is a trade name of a surfactant marketed by BASF Japan Ltd.

Test Example 1 (Oxygen consumption of human keratinocytes) Fresh mouse hepatocytes were immersed in Ringer's solution for homogenization, and the oxygen consumption of the homogenate was measured by an oxigraphy method. 20 extracts from beech tree shoots
As a result of examining the homogenate added with wt% as a test group and the control group added with Ringer's solution alone, it was found that the test group increased the oxygen consumption of cells by 71% with respect to the control group.

Test Example 2 (Keratinocyte protein synthesis) (Operation) Using keratinocyte basal medium without fetal bovine serum until colonization on the plastic plate in the medium,
Human keratinocytes were cultured by the Boyce method. ¹³ C
Methionine labeled with and 0.4% by weight of extract from beech tree shoots were added to the culture. Controls were those in which only methionine labeled with ¹³ C was added to the culture solution, and those in which neither the above extract nor methionine was added. After culturing for 72 hours, keratinocyte proteins were extracted and analyzed for amino acids by mass spectrometry. As a result, the test group to which the extract from beech tree larvae was added showed about 10% less protein synthesis when compared with the control group.
It was found to increase by 40%.

Formulation Example 1 (Cream) Ingredient Group A: Polyoxyethylene (8) Beeswax 8.00 (wt%) Octyldodecyl myristate 10.00 Isostearyl isostearate 10.00 Diethylene glycol monoethyl ether 5.00 Beech tree bud extract 2.00 Preservative and Fragrance Suitable amount Ingredient Group B: Purified water 100.00 Carbopol 934 0.20 Sodium hydroxide (10% aqueous solution) 0.40 After adding Carbopol to purified water and dispersing, 75 ℃
Heat to and add aqueous sodium hydroxide to it.
The solution was cooled to about 30-35 ° C., the components of Group B were sequentially added while stirring the warm solution, and then the whole was sufficiently kneaded to obtain a cream.

Test Example 3 (Effect on human skin) 3 on the medial side of the forearm of 20 healthy volunteer volunteers
A skin replica was created for each site. After that, for the next 4 weeks, apply each of the following creams to the replica collection site twice daily.
Two hours later, a skin replica of the above-mentioned replica collection site was created again. Part 1: Cream according to Formulation Example 1 (external preparation for study) Part 2: As in Formulation Example 1, but without the extract from beech tree larvae (control external preparation) Part 3: Image analysis of the microrelief of the untreated skin replica showed that no distinction was observed between the first and second replicas without treatment, and in the case of the control group. It was found that there is a significant difference between the two, but there is no significant difference, and in the case of the test group using the cream according to Formulation Example 1, the wrinkle level on the skin is reduced by about 10% on average. Therefore, the cream according to the present invention is presumed to be effective in preventing skin aging.

Test Example 4 (Skin Moisture Retention) In the same manner as in Test Example 3, healthy volunteer subjects 20
The study was conducted for 4 weeks at 3 sites on the inside of the forearm of a person. That is, the keratin water content at the measurement site was measured before the start of the test and 12 hours after the last application of the cream by means of a conniometer. As a result, the keratin water content at the site to which the control external preparation was applied was only slightly improved (several%) compared to the case of no treatment, but in the case of the test external preparation according to the present invention, It was also found that the keratin water content was about 30% higher on average when compared with the agent, and the moisturizing property was improved.

Formulation Example 2 (Tonic body lotion) Ingredient Group A: Mixture of polyoxyethylene (6) stearate and polyoxyethylene (32) stearate 7.00 (wt%) Glycerin stearate 0.50 Stearic acid 0.50 Propylene diperargonate Glycol 10.00 Octyldodecyl myristate 8.00 Ingredient group B: Glycerin 5.00 Purified water 100.00 Carbopol 941 0.10 Sodium hydroxide (10% aqueous solution) 0.20 Ingredient group C: Beech tree larvae extract 2.00 Preservatives and perfumes qs carb After adding the pole to purified water and dispersing, 75 ℃
Glycerin and an aqueous solution of sodium hydroxide are added thereto while heating and stirring. Cool the solution to about 30
At -35 ° C, the components of Group B were added sequentially while stirring this warm solution, then the components of Group C were added and further stirred to obtain the desired body lotion.

Formulation Example 3 (Shampoo) Purified water 100.00 (% by weight) Disodium lauryl ether sulfosuccinate 15.00 Sodium polyoxyethylene (2) Lauryl ether sulphate 15.00 Cocoamidopropyl betaine 7.00 Extraction from beech tree buds Substance 2.00 Coconut fatty acid diethanolamide 3.00 Preservative and perfume Suitable amount Citric acid (50% aqueous solution) 0.20 Sodium chloride 1.20 A shampoo was obtained by adding and mixing the components in the order of the above formulation. For citric acid, adjust the pH (5.5-6.0)
, And sodium chloride was used for viscosity adjustment.

[0022]

EFFECTS OF THE INVENTION The extract from beech shoots incorporated in the skin external preparation according to the present invention activates keratinocyte protein synthesis, thereby rejuvenating the skin and improving moisturizing properties. Therefore, the agent according to the present invention is effective in preventing skin aging.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI Technical display area A61K 9/08 A61K 9/08 M 9/107 9/107 S

Claims (3)

[Claims] 1. An external preparation for skin, which is mainly composed of an extract from beech tree buds. 2. The external preparation for skin according to claim 1, wherein the dosage form is cream, ointment, emulsion, gel or liquid. 3. The external preparation for skin according to claim 1, wherein the content of the extract is 0.1-50.0% by weight.