JP2011207769A - Cosmetic and skin care preparation for external use - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cosmetic and a skin care preparation for external use that effectively prevent or ameliorate generation of wrinkles.SOLUTION: The cosmetic and the skin care preparation for external use each comprises at least one moisturizing component selected from the group consisting of Glycine max, Angelica acutiloba, Gynostemma pentaphyllum, Rabdosia japonica, Alnus firma and valine, and a synthetic peptide exhibiting an anti-wrinkle action. In the cosmetic and the skin care preparation for external use, the synthetic peptide exhibits activity of competitively inhibiting, for example, binding of agrin to muscle-specific kinase (MuSK).

Description

  The present invention relates to a cosmetic and an external preparation for skin. More specifically, the present invention relates to a cosmetic containing a moisturizing component and a synthetic peptide having an anti-wrinkle action.

  The stratum corneum, located on the outermost layer of the skin, protects the living body from the contact and penetration of foreign substances such as bacteria and harmful substances, and also prevents the moisture from escaping from the body, thereby achieving a barrier function that keeps the skin healthy. Yes. When the stratum corneum barrier function is lowered, the amount of transepidermal water evaporated from the skin surface is increased, and the amount of stratum corneum is decreased. When the stratum corneum moisture content decreases, the skin exhibits a bulky rough state, and when this rough skin continues chronically, it causes wrinkles on the skin. In addition, stress caused by contraction of muscles (facial muscles) for creating facial expressions by moving the eyes, mouth, nose, etc. of the face is also considered to cause wrinkles on the skin.

  In order to prevent or improve the generation of wrinkles, conventionally moisturizing ingredients such as glycerin, natural moisturizing factor (NMF), and collagen derivatives have been used to prevent deterioration of the keratinous skin barrier function and to suppress transepidermal water evaporation. There has been proposed a method for increasing the moisture retention of the skin by applying to the skin.

  Moreover, in order to prevent or improve the generation of wrinkles, botulinum toxin such as BOTOX (registered trademark) has been applied locally (see Non-Patent Document 1). Botulinum toxin inhibits the release of acetylcholine by cleaving the protein involved in the release of acetylcholine (SNARE protein) into the synaptic space at the neuromuscular junction, thereby suppressing the contraction of facial muscles and reducing wrinkles. Has the effect of preventing or improving.

  Furthermore, in recent years, compounds that mimic the action of botulinum toxin have also been used to prevent or improve the generation of wrinkles. For example, Patent Document 1 discloses a peptide derived from an N-terminal fragment of synaptosome-related protein 25 (SNAP-25), which is one of SNARE proteins. This peptide competitively inhibits the formation of SNAP-25 SNARE protein complex, thereby inhibiting the release of acetylcholine, and is sold under the registered trademark “Argireline”. Patent Document 2 discloses a peptide derived particularly from the C-terminal part of the amino acid sequence of SNAP-25.

European Patent Application No. 1185524 International Publication No. 97/34620

"Treatment of glabellar frown lines with C. botulinum-A exotoxin." J. Dermatol. Surg. Oncol., 1992, Vol.18, No.1, p.17-21

  Since wrinkles are one of the obvious signs of aging, wrinkles that appear in the forehead, between the eyebrows, around the mouth and eyes are of great interest in cosmetics and are used to reduce or eliminate facial wrinkles. Development of cosmetics and external preparations for skin is expected. Therefore, a main object of the present invention is to provide a cosmetic and a skin external preparation capable of effectively preventing or improving the generation of wrinkles.

  The present inventors verified the effect of preventing or improving the generation of wrinkles by combining various moisturizing ingredients with a synthetic peptide having anti-wrinkle action, and synergistically anti-wrinkle effects by combining with specific moisturizing ingredients. As a result, the present invention has been completed.

That is, the present invention provides a cosmetic and a skin external preparation containing one or more moisturizing ingredients selected from the group consisting of soybean, toki, achazal, toki koshi, yashabushi and valine, and a synthetic peptide having an anti-wrinkle action. provide.
The cosmetic and the external preparation for skin preferably contain at least soybean and one or more moisturizing ingredients.
In this cosmetic and external preparation for skin, the synthetic peptide can have, for example, an activity that competitively inhibits the binding of agrin to muscle-specific kinase (MuSK). As such a synthetic peptide, a peptide represented by INCI name ACETYL HEXAPEPTIDE-30 can be used. This synthetic peptide is preferably a hexapeptide containing only aspartic acid, tyrosine, glutamic acid, leucine and arginine in the amino acid sequence.

  In the present invention, the term “moisturizing” refers to an effect that can be confirmed by measuring the amount of keratin moisture, as well as an effect that prevents or improves rough skin caused by dry skin, moisturization of the skin, a decrease in gloss and tension, etc. Including the broadest interpretation. The keratin water content can be measured by measuring electrical conductivity using a conventionally known measuring apparatus (for example, SKICON-200EX (manufactured by IBI S Co., Ltd.)).

  According to the present invention, a cosmetic and an external preparation for skin that can effectively prevent or improve the generation of wrinkles are provided.

  Hereinafter, preferred embodiments for carrying out the present invention will be described. In addition, embodiment described below shows an example of typical embodiment of this invention, and, thereby, the range of this invention is not interpreted narrowly.

1. Moisturizing component The cosmetic and the external preparation for skin according to the present invention contain one or more moisturizing components selected from the group consisting of soybean, toki, achacharu, tokikoshi, yashabushi and valine, and a synthetic peptide having an anti-wrinkle action. It is characterized by doing. One or more of these moisturizing ingredients can be blended in cosmetics or skin external preparations. Among these, it is particularly preferable to add soybean to enhance the anti-wrinkle effect. Below, each moisturizing component is demonstrated.

(1-1) Soybean Soybean is a leguminosae (Glycine) soybean (Glycine max), and seeds are particularly preferably used. Soybeans are pulverized soymilk, defatted soybeans, and soybean extracts extracted from these, hydrolyzed soybean protein obtained by treating these with acid, alkali, and enzyme, fermented soybean extract fermented with fungi and bacteria Things can be used. Of these, hydrolyzed soy protein is particularly preferred. Hydrolyzed soybean protein is, for example, a soybean protein solution obtained by dispersing defatted soybean in water, adjusting the pH, hydrolyzing it by adding a proteolytic enzyme, and then removing insoluble matter, or powdering it Are preferably used.

  The blending amount of the defatted soybean and other cosmetics and the external preparation for skin is preferably 0.00001 to 3.0% by mass, particularly preferably 0.00001 to 1.0% by mass as the dry weight in the total amount. If it is in this range, it can mix | blend a component stably and can exhibit the outstanding moisturizing effect and an anti-wrinkle effect. In addition, when mix | blending as a solution, if content of the dry solid content which is a solute is in the said range, solution concentration will not be limited.

(1-2) Touki, Achacharu, Hikiokoshi, Yashabushi Touki is a plant belonging to the family Umbelliferae, which is referred to as Toki under the name of herbal medicine. The place of origin is Japan. The main domestic production areas are Hokkaido, Nara, Wakayama, and Nagano, and others are from China and Korea. From China, Kara touki (Angelica sinensis (OLIV.)), From Japan, Toki (Yamazeri) (Angelica acutiloba KITAGAWA) (Daifuka Toki, Yamato Tokiwa) and Hokkaitouki (Hokkaido Toki) (Angelica acutiloba KITAGAWA var Sugiyamae HIKINO), South Korean products such as Angelica gigas NAKAI are used.

  Gynostemma pentaphyllum is a male and female heterogeneous vine that belongs to the genus Cucurbitaciae. It grows naturally in Japan, China and Southeast Asia, and leaves are used as medicinal herbs.

  Hikiokoshi is a plant of the genus Labiatae rabdosia, which is called a life-prolonging herb under the name of herbal medicine. In addition to raccoon (Rabdosia japonicus Hara), the same genera species, Rabdosia trichocarpa Hara, and turtle (Rabdosia umbrosa Hara var. Kameba Hara) are used.

  Yashabushi is a plant of the genus Betulaceae Alnus Mull. In addition to Alnus firma Sieb. Et Zucc., The same species species Alnus pendula Matsumura, Alnus sieboldana Matsumura, Alnus crispa (Aiton) Pursh. Maximowiczii Hara, Alnus pendula Matsumura Spach) Pupr.), Alnus japonica (Thunb.) Steud.), Etc. are used.

  Toki, Achacharu, Hikiokoshi and Yashabushi can be used raw or dried. The part to be used is not particularly limited as long as the object of the present invention is not impaired, and the whole plant or any part such as stem, bark, fruit, seed, rhizome, root bark, root, stem, branch, leaf, flower, etc. is selected. And it can be used in combination as appropriate. Of these, rhizomes are used for Toki, leaves are used for Achacharu, the aerial part is used for Hikikoshi, and fruits, pericarps or spikes are used for Yashabushi. Each part is used as a dry powder or, preferably, a solvent extract for convenience in handling during preparation.

  The solvent extract can be prepared by a conventional extraction method using an appropriate solvent. For example, the whole plant, or one or more of stems, bark, fruits, seeds, rhizomes, root barks, roots, stems, branches, leaves, flowers, etc., in a solvent at low or warm temperature for a predetermined time. It can be prepared by dipping or heating under reflux. The solvent extract may be subjected to filtration as necessary, and may be further subjected to concentration, purification / separation by ion exchange resin or liquid chromatography, and freeze-drying.

In general, one or two or more solvents that can be used for plant extraction can be freely selected and used as the solvent. Examples thereof include water, alcohols, glycols, ketones, esters, ethers, and halogenated carbons. Examples of alcohols include ethanol, methanol, and propanol. Examples of glycols include ethylene glycol, diethylene glycol, butylene glycol, and propylene glycol. Examples of ketones include acetone and methyl ethyl ketone. Examples of the esters include ethyl acetate, propyl acetate, and ethyl formate. These solvents may be used singly or as an aqueous solution, and a distribution method using any two or three or more mixed solvents may be appropriately employed.
Among these, in the present invention, a hydrophilic solvent is preferable, and it is particularly preferable to use water, ethanol, or butylene glycol alone or in combination. This is because these solvents have high cytotoxicity because of low cytotoxicity, and the obtained extract can be lyophilized for use.

  The solvent extract can be used in any form such as liquid, paste or gel. A liquid extract or the like can be dried and solidified, or dried by freeze drying, spray drying or the like and used as a powder.

  The blending amount of Toki, Achacharu, Hikiokoshi and Yashabushi in cosmetics and skin external preparations is preferably 0.00001 to 3.0% by mass, particularly preferably 0.00001 to 1.0% by mass in terms of dry weight in the total amount. It is. If it is in this range, it can mix | blend a component stably and can exhibit the outstanding moisturizing effect and an anti-wrinkle effect. When the extract is used as a solution, the concentration of the extract solution is not limited as long as the content of the dry solid content as a solute is within the above range.

(1-3) Valine Valine, which is one of the essential amino acids, can be used as crystals or powder. The blending amount of valine in the cosmetic and the external preparation for skin is preferably 0.00001 to 3.0% by mass, particularly preferably 0.00001 to 1.0% by mass as a solid weight in the total amount. If it is in this range, it can mix | blend a component stably and can exhibit the outstanding moisturizing effect and an anti-wrinkle effect.

2. Synthetic peptide having anti-wrinkle action The synthetic peptide blended in cosmetics and skin external preparations together with the above moisturizing ingredients is not particularly limited as long as it has an anti-wrinkle action, but preferably employs the compounds exemplified below. be able to.

(2-1) Peptides that inhibit the binding of agrin to muscle-specific kinase Examples of synthetic peptides include those that have the activity of competitively inhibiting the binding of agrin to muscle-specific kinase (MuSK). Muscle contraction is caused by acetylcholine released into the synaptic cleft from the axon end of the neuromuscular junction binds to acetylcholine receptors on muscle cells and accumulates the receptors. This accumulation of acetylcholine receptors involves agrin, a kind of proteoglycan released from axons. Agrin binds to a muscle-specific kinase (MuSK) on muscle cells to activate MuSK and become a signal necessary for synaptogenesis. By inhibiting the binding of agrin to MuSK, muscle contraction due to accumulation of acetylcholine receptors can be suppressed to prevent or improve the generation of wrinkles.

  As a synthetic peptide having an activity of competitively inhibiting the binding of agrin to MuSK, the name (INCI name) according to the International Nomenclature of Cosmetic Ingredients is “ACETYL HEXAPEPTIDE-30”, “Inyline” There are peptides manufactured under the registered trademark. This synthetic peptide contains only aspartic acid, tyrosine, glutamic acid, leucine and arginine in the amino acid sequence, and is a peptide modified with an acetyl group or an alkyl group.

(2-2) Peptide that inhibits formation of SNARE protein complex of synaptosome-related protein 25 Further, as a synthetic peptide, it has an activity to competitively inhibit the formation of SNARE protein complex of synaptosome-related protein 25 (SNAP-25). What has is also mentioned. The release of acetylcholine from the axon end to the synaptic cleft at the neuromuscular junction is caused by membrane fusion (exocytosis) between the synaptic vesicle encapsulating acetylcholine in the nerve cell. This exocytosis involves synaptobrevin (VAMP / Synaptobrevin) on the surface of synaptic vesicles, syntaxin on the cell membrane side and SNAP-25 (SNARE protein), and these three SNARE proteins are complexed. Membrane fusion and neurotransmitter release are performed by forming the body. By inhibiting the formation of the SNAP-25 SNARE protein complex, muscle contraction due to the release of acetylcholine can be suppressed, and the generation of wrinkles can be prevented or improved.

(2-3) Peptide that inhibits binding of enkephalin to enkephalin receptor Furthermore, synthetic peptides include those that have an activity to competitively inhibit binding of enkephalin to the enkephalin receptor. The release of acetylcholine by exocytosis from the axon end requires the influx of calcium ions into the cytoplasm of neurons, in addition to the formation of SNARE protein complexes. Enkephalin binds to the receptor and activates the trimeric G protein complex, causing the release of calcium ions from the intracellular reservoir via the inositol phosphate receptor. By inhibiting the binding of enkephalin to the enkephalin receptor, muscle contraction due to the release of acetylcholine can be suppressed, and the generation of wrinkles can be prevented or improved.

  The blending amount of these synthetic peptides in cosmetics and external preparations for skin is preferably 0.000001 to 1.0% by mass, particularly preferably 0.00001 to 0.1% by mass, as a solid weight in the total amount. Within this range, the components can be blended stably and an excellent anti-wrinkle effect can be exhibited.

3. Additives In addition to the above moisturizing ingredients and synthetic peptides, various commonly used ingredients can be appropriately added to the cosmetics and skin external preparations according to the present invention as long as the effects of the present invention are not impaired. it can. Additives include bases, colorants, extender pigments, colored pigments, surfactants, UV absorbers, antioxidants, antiseptic / antifungal agents, alcohols, polyhydric alcohols, water, oils, thickeners, wetting agents , Powders, chelating agents, pH adjusters, enzymes, various medicinal agents, extracts derived from animals, plants and microorganisms, and fragrances.

  The cosmetics can be applied to all forms of cosmetics. For example, skin lotion, milky lotion, cream, eye cream, beauty essence, massage, pack, hand cream, body cream, face wash, skin care cosmetics, shampoo, hair treatment, hair styling agent, hair nourishing agent, hair restorer, etc. It can be applied to hair cosmetics, powder foundations, liquid foundations, makeup base cosmetics, white powder, concealers, lipsticks, blushers, eye shadows, eyeliner makeup cosmetics, sunscreens and the like.

  The external preparation for skin can be applied to external preparations of any dosage form. For example, apply to external preparations such as external preparations, external preparations, creams, ointments, sprays, nasal drops, liniments, lotions, haps, plasters, sprays, aerosols, bath preparations, etc. Can do.

  The present invention will be described more specifically with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

[Test Example 1: Evaluation of anti-wrinkle effect of cream]
A cream prepared according to the formulation shown in “Table 1” and “Table 2” was applied to the eyes, and a use test for comparing the wrinkle improvement effect before and after use was conducted for one month. In each example and comparative example, 5 panel members each 35 to 55 years old were used.

The cream was prepared by the following manufacturing method.
A: Components (1) to (8) are mixed and dissolved uniformly at 70 ° C.
B: Components (10) and (18) are mixed and heated to 70 ° C.
C: B is added to A and emulsified.
D: After cooling, components (9) and (11) to (17) are added.

Each moisturizing component was prepared by the method described below.
(1) Soybean
[Production Example 1: Soybean extract]
5 kg of purified water is added to 1 kg of dried soybean Glycine max Merrill (Leguminosae) seeds, soaked for 12 hours, and then extracted at 80 ° C. for 8 hours. The filtrate obtained by filtering the extract was dried under reduced pressure. The obtained dried product was prepared by dissolving in a 30% by mass 1,3-butylene glycol aqueous solution so as to have a concentration of 3% by mass.
[Production Example 2: Hydrolyzed soybean extract]
1 kg of dried soybean Glycine max Merrill (Leguminosae) is pulverized, 5 L of purified water is added, and the mixture is extracted at 80 ° C. for 3 hours. The extract was hydrolyzed with protease at 40 ° C. for 3 hours, heated to 80 ° C. to inactivate the protease, and then filtered. The obtained filtrate was dried under reduced pressure, and the obtained dried product was prepared by dissolving in a 20% by mass dipropylene glycol aqueous solution so as to have a concentration of 2% by mass.
[Production Example 3: Fermented soybean extract]
1 kg of dried soybean Glycine max Merrill (Leguminosae) is pulverized, 5 L of purified water is added, and the mixture is extracted at 80 ° C. for 3 hours. Add lactic acid bacteria (Lactbacillus delbrueckii) to the extract, incubate for 3 days, and filter and sterilize the supernatant. It was prepared by adding an appropriate amount of paraben. When this product was measured separately, the solid content concentration was 8% by mass.
(2) Toki To 100 g of ground pulverized product of Angelica acutiloba (Sieb. Et Zucc.) Kitagawa, 100 ml of 50 mass% ethanol was added and extracted at room temperature for 3 days. The filtrate obtained by filtering the extract was dried under reduced pressure, and the resulting dried product was prepared as a 5% by mass solution using a solvent in which water: ethanol: propylene glycol = 1: 1: 1 was mixed. did.
(3) Amateur Gynostemma pentaphyllum Makino (Cucurubitaceae) 1 kg of leaves, add 10 L of purified water, heat and reflux, and filter under reduced pressure at 50 ° C. Used in addition.
(4) Hikiokoshi 1 kg of the above ground portion of Isodon japonicus Hara is shredded, 5 L of 50 mass% ethanol aqueous solution is added and heated to reflux for 5 hours, followed by filtration. The filtrate was allowed to stand in a cool dark place for 3 days, and after removing the precipitate, it was dried under reduced pressure. The obtained dried product was dissolved again in 50 mass ethanol aqueous solution so as to be a 2 mass% solution.
(5) Yashabushi 1 L of 70 volume% hydrous ethyl alcohol was added to 100 g of Alnus firma sieb.Et Zucc. And extracted for 3 days at room temperature. After the extract was filtered, the solvent was distilled off to obtain 0.7 g of a citrus fruit extract.
(6) Valine L-valine made by Ajinomoto Co. was used.
(7) Synthetic peptides
INYLINE (registered trademark) manufactured by LIPOTEC or the essence of INCI name ACETYL HEXAPEPTIDE-30 was used.

Based on the evaluation criteria shown below, the wrinkle improvement effect was evaluated by scoring the degree of improvement of the eye wrinkles before and after use on the basis of Comparative Example 1 and calculating the average score.
Evaluation score 6: Excellent 5: Excellent 4: Slightly excellent 3: No change 2: Slightly inferior 1: Inferior 0: Very inferior

  The results are shown in “Table 1” and “Table 2”. Compared to the cream according to Comparative Example 3 containing only the synthetic peptide and the cream according to Comparative Example 1 containing only the moisturizing component such as soybean extract, Examples 1 to 6 containing both the moisturizing component and the synthetic peptide were used. In such a cream, a significant wrinkle improving effect was recognized. Especially the cream which concerns on Example 1 which mix | blended the soybean extract and the synthetic peptide showed the remarkable wrinkle improvement effect compared with others.

[Test Example 2: Evaluation of anti-wrinkle effect of lotion]
A lotion prepared according to the formulations shown in “Table 3” and “Table 4” was applied to the eyes, and a use test was conducted for 1 month to compare the wrinkle improvement effect before and after use. In each example and comparative example, 5 panel members each 35 to 55 years old were used. The evaluation of the wrinkle improvement effect was performed by scoring on the basis of Comparative Example 9 as in Test Example 1.

The lotion was prepared by the following production method.
A: Components (1) to (5) are uniformly dissolved.
B: Components (6) to (16) are uniformly dissolved.
C: A is added to B.

  The results are shown in “Table 3” and “Table 4”. Example 7 where both moisturizing ingredients and synthetic peptides were blended, compared to lotions according to Comparative Examples 10 to 15 containing only the moisturizing ingredients such as the lotion and soy extract according to Comparative Example 9 containing only the synthetic peptides In the lotion according to ˜15, a significant wrinkle improvement effect was recognized. In particular, the lotion according to Example 7 in which the soybean extract and the synthetic peptide were blended showed a synergistic wrinkle improving effect as compared with the lotion in which the soybean extract or the synthetic peptide was blended alone.

[Test Example 3: Evaluation of anti-wrinkle effect of emulsion]
An emulsion prepared according to the formulation shown in “Table 5” and “Table 6” was applied to the eye and a use test was conducted for 1 month to compare the wrinkle improvement effect before and after use. In each example and comparative example, 5 panel members each 35 to 55 years old were used. The evaluation of the wrinkle improvement effect was performed by scoring on the basis of Comparative Example 16 as in Test Example 1.

The emulsion was prepared by the following production method.
A: Components (1) to (9) are heated to 70 ° C. and dissolved uniformly.
B: Components (12) to (14) and (22) are heated to 70 ° C. and dissolved uniformly.
C: B is added to A and emulsified.
D: After cooling, components (10), (11), (15) to (21) are added.

  The results are shown in “Table 5” and “Table 6”. Compared to the emulsion according to Comparative Example 16 in which only the synthetic peptide is blended and the emulsion according to Comparative Examples 17 and 18 in which only the soybean extract is blended, in the emulsions according to Examples 16 to 21 in which both the moisturizing component and the synthetic peptide are blended , A significant wrinkle improvement effect was observed. Furthermore, the wrinkle improvement effect was further enhanced in the emulsions according to Examples 22 to 26, in which the synthetic peptide was combined with a moisturizing component such as soybean extract and other Toki extract.

[Formulation Example 1: Pack]
(Ingredient) (%)
(1) Polyvinyl alcohol 15.0
(2) Silicic anhydride 0.5
(3) Polyoxypropylene (10) methyl glucoside 5.0
(4) Ethanol 20.0
(5) INYLINE 0.05
(6) Soybean extract of Production Example 1 0.1
(7) Soybean extract of Production Example 2 0.1
(8) Dipotassium glycyrrhizinate 0.1
(9) Glycerin 5.0
(10) Methylparaben 0.1
(11) Perfume 0.02
(12) Remaining amount of purified water

(Production method)
A: Components (1) to (4) and (11) are mixed and dissolved.
B: Components (5) to (10) and (12) are mixed and dissolved.
C: A and B were mixed and uniformed to obtain a pack.

[Formulation Example 2: Ointment]
(Ingredient) (%)
(1) Stearic acid 15.0
(2) Cetanol 7.0
(3) Triethanolamine 2.0
(4) Glycerin 7.0
(5) Synthetic peptide (ACETYL HEXAPEPTIDE-30) 0.1
(6) Soybean extract of Production Example 3 3.0
(7) Tocopherol acetate 1.0
(8) Purified water remaining

(Production method)
A: A part of the components (3), (4) and (8) is heated and mixed and kept at 75 ° C.
B: Components (1) and (2) are heated and mixed and kept at 75 ° C.
C: Gradually add A to B.
D: (5) to (7) dissolved in the remainder of (8) were added while cooling C to obtain an ointment.

[Formulation Example 3: Daytime serum]
(Ingredient) (%)
(1) Polyoxyalkylene-modified organopolysiloxane 2.0
(2) Dimethylpolysiloxane 5.0
(3) Isotridecyl isononanoate 5.0
(4) 2-Ethylhexyl paramethoxycinnamate 5.0
(5) Methylenebisbenzotriazolyltetramethyl butylphenol 1.0
(6) Silicone-treated fine particle zinc oxide 8.0
(7) Polystyrene powder 2.0
(8) Decamethylcyclopentasiloxane 10.0
(9) Methyltrimethicone 10.0
(10) Preservative appropriate amount (11) perfume appropriate amount (12) dipropylene glycol 3.0
(13) Ethanol 10.0
(14) INYLINE 0.01
(15) Toki extract 0.01
(16) Amateur extract 0.01
(17) Vitamin C glucoside 0.5
(18) Purified water remaining

(Production method)
A: Components (1) to (11) are uniformly dissolved.
B: Components (12) to (18) are uniformly dissolved.
C: B is added to A and emulsified.

[Formulation Example 4: Water-in-oil foundation]
(Ingredient) (%)
(1) Lecithin-treated titanium oxide 8.0
(2) Lecithin-treated iron oxide 2.0
(3) Lecithin-treated yellow iron oxide 1.3
(4) Lecithin-treated black iron oxide 0.1
(5) Silicone-treated talc 4.0
(6) Silicone-treated mica 2.0
(7) Polyoxyethylene-modified silicone 2.0
(8) Hydrogenated lecithin 0.2
(9) Organically modified ammonium hectorite 1.0
(10) Dextrin palmitate 0.5
(11) Inulin stearate 0.5
(12) Dipentaerythlit fatty acid ester 1.0
(13) Partially cross-linked methylpolysiloxane 2.0
(14) Neopentyl glycol dicaprate 4.0
(15) Hydrogenated polyisobutene 10.0
(16) Decamethylcyclopentasiloxane 10.0
(17) Perfume appropriate amount (18) 1,3-butylene glycol 3.0
(19) Ethanol 5.0
(20) Preservative appropriate amount (21) Sodium chloride 0.5
(22) INYLINE 0.02
(23) Toad extract 0.1
(24) Yashabushi extract 0.1
(25) Vitamin A palmitate 0.02
(26) Purified water remaining

(Production method)
A: Components (10) to (12) are heated to 90 ° C. and dissolved uniformly.
B: Components (1) to (9) are added to A and uniformly dispersed by a three-roller.
C: Components (13) to (16) are added to B and mixed uniformly.
D: Components (18) to (26) are mixed uniformly.
E: While stirring C with a disper mixer, D is added and emulsified.
F: Component (17) was added to E to obtain a foundation.

  The packs, ointments, daytime serums, and water-in-oil foundations prepared in each of the above formulation examples are stable without any discoloration / odor change and precipitation / separation. Excellent wrinkle improvement effect.

  The cosmetic and the external preparation for skin according to the present invention can effectively prevent or improve the generation of wrinkles, especially for reducing or removing wrinkles that appear around the forehead, eyebrows, mouth and eyes. It can be used effectively as a cosmetic.

Claims (6)

  A cosmetic comprising one or more moisturizing ingredients selected from the group consisting of soybean, toki, achazul, toki koshi, yashabushi and valine, and a synthetic peptide having an anti-wrinkle action.   A skin external preparation containing one or more moisturizing ingredients selected from the group consisting of soybean, touki, achazal, toki koshi, yashabushi and valine, and a synthetic peptide having an anti-wrinkle action.   The cosmetic according to claim 1 or the external preparation for skin according to claim 2, wherein the moisturizing component contains at least one selected from the group consisting of Toki, Achacharu, Hikikoshi, Yashabushi and Valine and soybean.   The cosmetic or skin external preparation according to any one of claims 1 to 3, wherein the synthetic peptide has an activity of competitively inhibiting the binding of agrin to muscle-specific kinase (MuSK).   The cosmetic or external preparation for skin according to any one of claims 1 to 3, wherein the synthetic peptide is represented by the INCI name ACETYL HEXAPEPTIDE-30.   The cosmetic or external preparation for skin according to any one of claims 1 to 3, wherein the synthetic peptide is a hexapeptide containing only aspartic acid, tyrosine, glutamic acid, leucine and arginine in the amino acid sequence.